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Journal of Extracellular Vesicles

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https://www.readbyqxmd.com/read/28473886/unique-molecular-profile-of-exosomes-derived-from-primary-human-proximal-tubular-epithelial-cells-under-diseased-conditions
#1
Xiangju Wang, Ray Wilkinson, Katrina Kildey, Jeremy Potriquet, Jason Mulvenna, Richard J Lobb, Andreas Möller, Nicole Cloonan, Pamela Mukhopadhyay, Andrew J Kassianos, Helen Healy
Human proximal tubular epithelial cells (PTEC) of the kidney are known to respond to and mediate the disease process in a wide range of kidney diseases, yet their exosomal production and exosome molecular cargo remain a mystery. Here we investigate, for the first time, the production and molecular content of exosomes derived from primary human PTEC cultured under normal and diseased conditions representing a spectrum of in vivo disease severity from early inflammation, experienced in multiple initial kidney disease states, through to hypoxia, frequently seen in late stage chronic kidney disease (CKD) due to fibrosis and vascular compromise...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28473885/prospects-and-limitations-of-antibody-mediated-clearing-of-lipoproteins-from-blood-plasma-prior-to-nanoparticle-tracking-analysis-of-extracellular-vesicles
#2
Morten Mørk, Aase Handberg, Shona Pedersen, Malene M Jørgensen, Rikke Bæk, Morten K Nielsen, Søren R Kristensen
Introduction: Nanoparticle tracking analysis (NTA) enables measurement of extracellular vesicles (EVs) but lacks the ability to distinct between EVs and lipoproteins which are abundantly present in blood plasma. Limitations in ultracentrifugation and size exclusion chromatography applied for EV isolation may result in inadequate EV purification and preservation. In this proof of concept study, we aimed to evaluate the potential of antibody-mediated removal of lipoproteins from plasma prior to extracellular vesicle (EV) analysis by nanoparticle tracking analysis (NTA)...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28473884/characterisation-of-adipocyte-derived-extracellular-vesicle-subtypes-identifies-distinct-protein-and-lipid-signatures-for-large-and-small-extracellular-vesicles
#3
Maëva Durcin, Audrey Fleury, Emiliane Taillebois, Grégory Hilairet, Zuzana Krupova, Céline Henry, Sandrine Truchet, Martin Trötzmüller, Harald Köfeler, Guillaume Mabilleau, Olivier Hue, Ramaroson Andriantsitohaina, Patrice Martin, Soazig Le Lay
Extracellular vesicles (EVs) are biological vectors that can modulate the metabolism of target cells by conveying signalling proteins and genomic material. The level of EVs in plasma is significantly increased in cardiometabolic diseases associated with obesity, suggesting their possible participation in the development of metabolic dysfunction. With regard to the poor definition of adipocyte-derived EVs, the purpose of this study was to characterise both qualitatively and quantitatively EVs subpopulations secreted by fat cells...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28473883/characterisation-of-extracellular-vesicle-subsets-derived-from-brain-endothelial-cells-and-analysis-of-their-protein-cargo-modulation-after-tnf-exposure
#4
Vito Dozio, Jean-Charles Sanchez
Little is known about the composition and functional differences between extracellular vesicle (EV) subsets, such as microvesicles (MVs) and exosomes (EXOs), nor to what extent their cargo reflects the phenotypic state of the cell of origin. Brain endothelial cells are the constitutive part of the blood-brain barrier (BBB), a selective barrier that maintains brain homeostasis. BBB impairment is associated with several neuroinflammatory diseases with the pro-inflammatory cytokine tumour necrosis factor (TNF) often playing a key role...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28386391/pellet-free-isolation-of-human-and-bovine-milk-extracellular-vesicles-by-size-exclusion-chromatography
#5
Kristine Blans, Maria S Hansen, Laila V Sørensen, Michael L Hvam, Kenneth A Howard, Arne Möller, Lars Wiking, Lotte B Larsen, Jan T Rasmussen
Studies have suggested that nanoscale extracellular vesicles (EV) in human and bovine milk carry immune modulatory properties which could provide beneficial health effects to infants. In order to assess the possible health effects of milk EV, it is essential to use isolates of high purity from other more abundant milk structures with well-documented bioactive properties. Furthermore, gentle isolation procedures are important for reducing the risk of generating vesicle artefacts, particularly when EV subpopulations are investigated...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28386390/acute-lymphoblastic-leukaemia-cells-produce-large-extracellular-vesicles-containing-organelles-and-an-active-cytoskeleton
#6
Suzanne M Johnson, Clare Dempsey, Catriona Parker, Aleksandr Mironov, Helen Bradley, Vaskar Saha
Extracellular vesicles have been described in non-paracrine cellular interactions in cancer. We report a similar phenomenon in B-cell precursor (BCP) acute lymphoblastic leukaemia (ALL). Using advanced microscopy and high throughput screening, we further characterise a subset of large vesicles (LEVs) identified in cell lines, murine models of human BCP-ALL and clinical samples. Primary ALL blasts and cell lines released heterogeneous anucleate vesicles <6 micron into extracellular fluids. Larger LEVs were enclosed in continuous membranes, contained intact organelles and demonstrated an organised cytoskeleton...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28326171/large-scale-isolation-and-cytotoxicity-of-extracellular-vesicles-derived-from-activated-human-natural-killer-cells
#7
Ambrose Y Jong, Chun-Hua Wu, Jingbo Li, Jianping Sun, Muller Fabbri, Alan S Wayne, Robert C Seeger
Extracellular vesicles (EVs) have been the focus of great interest, as they appear to be involved in numerous important cellular processes. They deliver bioactive macromolecules such as proteins, lipids, and nucleic acids, allowing intercellular communication in multicellular organisms. EVs are secreted by all cell types, including immune cells such as natural killer cells (NK), and they may play important roles in the immune system. Currently, a large-scale procedure to obtain functional NK EVs is lacking, limiting their use clinically...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28326170/obstacles-and-opportunities-in-the-functional-analysis-of-extracellular-vesicle-rna-an-isev-position-paper
#8
Bogdan Mateescu, Emma J K Kowal, Bas W M van Balkom, Sabine Bartel, Suvendra N Bhattacharyya, Edit I Buzás, Amy H Buck, Paola de Candia, Franklin W N Chow, Saumya Das, Tom A P Driedonks, Lola Fernández-Messina, Franziska Haderk, Andrew F Hill, Jennifer C Jones, Kendall R Van Keuren-Jensen, Charles P Lai, Cecilia Lässer, Italia di Liegro, Taral R Lunavat, Magdalena J Lorenowicz, Sybren L N Maas, Imre Mäger, Maria Mittelbrunn, Stefan Momma, Kamalika Mukherjee, Muhammed Nawaz, D Michiel Pegtel, Michael W Pfaffl, Raymond M Schiffelers, Hidetoshi Tahara, Clotilde Théry, Juan Pablo Tosar, Marca H M Wauben, Kenneth W Witwer, Esther N M Nolte-'t Hoen
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28326169/circulating-ll37-targets-plasma-extracellular-vesicles-to-immune-cells-and-intensifies-beh%C3%A3-et-s-disease-severity
#9
Tamer Kahraman, Gozde Gucluler, Ismail Simsek, Fuat Cem Yagci, Muzaffer Yildirim, Can Ozen, Ayhan Dinc, Mayda Gursel, Lolai Ikromzoda, Tolga Sutlu, Stephen Gay, Ihsan Gursel
Behçet's disease (BD) activity is characterised by sustained, over-exuberant immune activation, yet the underlying mechanisms leading to active BD state are poorly defined. Herein, we show that the human cathelicidin derived antimicrobial peptide LL37 associates with and directs plasma extracellular vesicles (EV) to immune cells, thereby leading to enhanced immune activation aggravating BD pathology. Notably, disease activity was correlated with elevated levels of circulating LL37 and EV plasma concentration...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28326168/ribonucleic-artefacts-are-some-extracellular-rna-discoveries-driven-by-cell-culture-medium-components
#10
REVIEW
Juan Pablo Tosar, Alfonso Cayota, Erez Eitan, Marc K Halushka, Kenneth W Witwer
In a recently published study, Anna Krichevsky and colleagues raise the important question of whether results of in vitro extracellular RNA (exRNA) studies, including extracellular vesicle (EV) investigations, are confounded by the presence of RNA in cell culture medium components such as foetal bovine serum (FBS). The answer, according to their data, is a resounding "yes". Even after lengthy ultracentrifugation to remove bovine EVs from FBS, the majority of exRNA in FBS remained. Although technical factors may affect the degree of depletion, residual EVs and exRNA in FBS could influence the conclusions of in vitro studies: certainly, for secreted RNA, and possibly also for cell-associated RNA...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28326167/residual-urinary-extracellular-vesicles-in-ultracentrifugation-supernatants-after-hydrostatic-filtration-dialysis-enrichment
#11
Luca Musante, Dorota Tataruch-Weinert, Dontscho Kerjaschki, Michael Henry, Paula Meleady, Harry Holthofer
Urinary extracellular vesicles (UEVs) appear an ideal source of biomarkers for kidney and urogenital diseases. The majority of protocols designed for their isolation are based on differential centrifugation steps. However, little is still known of the type and amount of vesicles left in the supernatant. Here we used an isolation protocol for UEVs which uses hydrostatic filtration dialysis as first pre-enrichment step, followed by differential centrifugation. Transmission electron microscopy (TEM), mass spectrometry (MS), western blot, ELISA assays and tuneable resistive pulse sensing (TRPS) were used to characterise and quantify UEVs in the ultracentrifugation supernatant...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28326166/trail-delivery-by-msc-derived-extracellular-vesicles-is-an-effective-anticancer-therapy
#12
ZhengQiang Yuan, Krishna K Kolluri, Kate H C Gowers, Sam M Janes
Extracellular vesicles (EVs) are lipid membrane-enclosed nanoparticles released by cells. They mediate intercellular communication by transferring biological molecules and therefore have potential as innovative drug delivery vehicles. TNF-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis of cancer cells. Unfortunately, the clinical application of recombinant rTRAIL has been hampered by its low bioavailability and resistance of cancer cells. EV-mediated TRAIL delivery may circumvent these problems...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/27989272/the-international-society-for-extracellular-vesicles-launches-the-first-massive-open-online-course-on-extracellular-vesicles
#13
EDITORIAL
Cecilia Lässer, Clotilde Théry, Edit I Buzás, Suresh Mathivanan, Weian Zhao, Yong Song Gho, Jan Lötvall
The International Society for Extracellular Vesicles (ISEV) has organised its first educational online course for students and beginners in the field of extracellular vesicles (EVs). This course, "Basics of Extracellular Vesicles," uses recorded lectures from experts in the field and will be open for an unlimited number of participants. The course is divided into 5 modules and can be accessed at www.coursera.org/learn/extracellular-vesicles. The first module is an introduction to the field covering the nomenclature and history of EVs...
2016: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/27863537/high-resolution-proteomic-and-lipidomic-analysis-of-exosomes-and-microvesicles-from-different-cell-sources
#14
Reka A Haraszti, Marie-Cecile Didiot, Ellen Sapp, John Leszyk, Scott A Shaffer, Hannah E Rockwell, Fei Gao, Niven R Narain, Marian DiFiglia, Michael A Kiebish, Neil Aronin, Anastasia Khvorova
Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for use in diagnostics, therapeutics and drug delivery. However, little is known about the relationship of protein and lipid composition of EVs and their source cells. Here, we report high-resolution lipidomic and proteomic analyses of exosomes and MVs derived by differential ultracentrifugation from 3 different cell types: U87 glioblastoma cells, Huh7 hepatocellular carcinoma cells and human bone marrow-derived mesenchymal stem cells (MSCs)...
2016: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/27802845/techniques-used-for-the-isolation-and-characterization-of-extracellular-vesicles-results-of-a-worldwide-survey
#15
Chris Gardiner, Dolores Di Vizio, Susmita Sahoo, Clotilde Théry, Kenneth W Witwer, Marca Wauben, Andrew F Hill
Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype between different populations of EVs. Little is known about current practices for the isolation, purification and characterization of EVs...
2016: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/27702427/presence-of-cx43-in-extracellular-vesicles-reduces-the-cardiotoxicity-of-the-anti-tumour-therapeutic-approach-with-doxorubicin
#16
Tania Martins-Marques, Maria Joao Pinho, Monica Zuzarte, Carla Oliveira, Paulo Pereira, Joost P G Sluijter, Celia Gomes, Henrique Girao
Extracellular vesicles (EVs) are major conveyors of biological information, mediating local and systemic cell-to-cell communication under physiological and pathological conditions. These endogenous vesicles have been recognized as prominent drug delivery vehicles of several therapeutic cargoes, including doxorubicin (dox), presenting major advantages over the classical approaches. Although dox is one of the most effective anti-tumour agents in the clinical practice, its use is very often hindered by its consequent dramatic cardiotoxicity...
2016: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/27680302/oncosomes-large-and-small-what-are-they-where-they-came-from
#17
LETTER
Brian Meehan, Janusz Rak, Dolores Di Vizio
No abstract text is available yet for this article.
2016: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/27680301/a-standardized-method-to-determine-the-concentration-of-extracellular-vesicles-using-tunable-resistive-pulse-sensing
#18
Robert Vogel, Frank A W Coumans, Raluca G Maltesen, Anita N Böing, Katherine E Bonnington, Marike L Broekman, Murray F Broom, Edit I Buzás, Gunna Christiansen, Najat Hajji, Søren R Kristensen, Meta J Kuehn, Sigrid M Lund, Sybren L N Maas, Rienk Nieuwland, Xabier Osteikoetxea, Rosalie Schnoor, Benjamin J Scicluna, Mitch Shambrook, Jeroen de Vrij, Stephen I Mann, Andrew F Hill, Shona Pedersen
BACKGROUND: Understanding the pathogenic role of extracellular vesicles (EVs) in disease and their potential diagnostic and therapeutic utility is extremely reliant on in-depth quantification, measurement and identification of EV sub-populations. Quantification of EVs has presented several challenges, predominantly due to the small size of vesicles such as exosomes and the availability of various technologies to measure nanosized particles, each technology having its own limitations. MATERIALS AND METHODS: A standardized methodology to measure the concentration of extracellular vesicles (EVs) has been developed and tested...
2016: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/27664155/extracellular-vesicles-are-present-in-mouse-lymph-and-their-level-differs-in-atherosclerosis
#19
Andreea Milasan, Nicolas Tessandier, Sisareuth Tan, Alain Brisson, Eric Boilard, Catherine Martel
The lymphatic system works in close collaboration with the cardiovascular system to preserve fluid balance throughout the body and is essential for the trafficking of antigen-presenting cells and lymphocytes to lymphoid organs. Recent findings have associated lymphatic dysfunction with the pathogenesis of cardiovascular-related diseases such as atherosclerosis, inflammation and obesity. Whether lymphatic dysfunction is a cause or a consequence of these diseases, as well as how, is under intensive investigation...
2016: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/27576678/mirna-profiling-of-circulating-epcam-extracellular-vesicles-promising-biomarkers-of-colorectal-cancer
#20
Marie Stampe Ostenfeld, Steffen Grann Jensen, Dennis Kjølhede Jeppesen, Lise-Lotte Christensen, Stine Buch Thorsen, Jan Stenvang, Michael Lykke Hvam, Anni Thomsen, Peter Mouritzen, Mads Heilskov Rasmussen, Hans Jørgen Nielsen, Torben Falck Ørntoft, Claus Lindbjerg Andersen
Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. These contain biomolecules, including proteins and microRNAs (miRNAs). Both circulating EVs and miRNAs have received much attention as biomarker candidates for non-invasive diagnostics. Here we describe a sensitive analytical method for isolation and subsequent miRNA profiling of epithelial-derived EVs from blood samples of patients with colorectal cancer (CRC). The epithelial-derived EVs were isolated by immunoaffinity-capture using the epithelial cell adhesion molecule (EpCAM) as marker...
2016: Journal of Extracellular Vesicles
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