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Journal of Extracellular Vesicles

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https://www.readbyqxmd.com/read/29887978/evaluation-of-serum-extracellular-vesicle-isolation-methods-for-profiling-mirnas-by-next-generation-sequencing
#1
Dominik Buschmann, Benedikt Kirchner, Stefanie Hermann, Melanie Märte, Christine Wurmser, Florian Brandes, Stefan Kotschote, Michael Bonin, Ortrud K Steinlein, Michael W Pfaffl, Gustav Schelling, Marlene Reithmair
Extracellular vesicles (EVs) are intercellular communicators with key functions in physiological and pathological processes and have recently garnered interest because of their diagnostic and therapeutic potential. The past decade has brought about the development and commercialization of a wide array of methods to isolate EVs from serum. Which subpopulations of EVs are captured strongly depends on the isolation method, which in turn determines how suitable resulting samples are for various downstream applications...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29760869/metabolic-alterations-in-urine-extracellular-vesicles-are-associated-to-prostate-cancer-pathogenesis-and-progression
#2
Marc Clos-Garcia, Ana Loizaga-Iriarte, Patricia Zuñiga-Garcia, Pilar Sánchez-Mosquera, Ana Rosa Cortazar, Esperanza González, Verónica Torrano, Cristina Alonso, Miriam Pérez-Cormenzana, Aitziber Ugalde-Olano, Isabel Lacasa-Viscasillas, Azucena Castro, Felix Royo, Miguel Unda, Arkaitz Carracedo, Juan M Falcón-Pérez
Urine contains extracellular vesicles (EVs) that concentrate molecules and protect them from degradation. Thus, isolation and characterisation of urinary EVs could increase the efficiency of biomarker discovery. We have previously identified proteins and RNAs with differential abundance in urinary EVs from prostate cancer (PCa) patients compared to benign prostate hyperplasia (BPH). Here, we focused on the analysis of the metabolites contained in urinary EVs collected from patients with PCa and BPH. Targeted metabolomics analysis of EVs was performed by ultra-high-performance liquid chromatography-mass spectrometry...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29713426/extracellular-vesicles-from-bone-marrow-derived-mesenchymal-stromal-cells-support-ex-vivo-survival-of-human-antibody-secreting-cells
#3
Doan C Nguyen, Holly C Lewis, Chester Joyner, Vivien Warren, Haopeng Xiao, Haydn T Kissick, Ronghu Wu, Jacques Galipeau, F Eun-Hyung Lee
Extracellular vesicles (EVs) from bone marrow (BM)-derived mesenchymal stromal cells (BM-MSC) are novel mechanisms of cell-cell communication over short and long distances. BM-MSC have been shown to support human antibody secreting cells (ASC) survival ex vivo , but whether the crosstalk between the MSC-ASC interaction can occur via EVs is not known. Thus, we evaluated the role of EVs in ASC survival and IgG secretion. EVs were isolated from irradiated and non-irradiated primary BM-MSC and were quantified. They were further characterized by electron microscopy (EM) and CD63 and CD81 immuno-gold EM staining...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29696081/proteomic-analysis-reveals-different-composition-of-extracellular-vesicles-released-by-two-trypanosoma-cruzi-strains-associated-with-their-distinct-interaction-with-host-cells
#4
Kleber Silva Ribeiro, Camilla Ioshida Vasconcellos, Rodrigo Pedro Soares, Maria Tays Mendes, Cameron C Ellis, Marcela Aguilera-Flores, Igor Correia de Almeida, Sergio Schenkman, Leo Kei Iwai, Ana Claudia Torrecilhas
Trypanosoma cruzi , the aetiologic agent of Chagas disease, releases vesicles containing a wide range of surface molecules known to affect the host immunological responses and the cellular infectivity. Here, we compared the secretome of two distinct strains (Y and YuYu) of T. cruzi , which were previously shown to differentially modulate host innate and acquired immune responses. Tissue culture-derived trypomastigotes of both strains secreted extracellular vesicles (EVs), as demonstrated by electron scanning microscopy...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29696080/therapeutic-effects-of-serum-extracellular-vesicles-in-liver-fibrosis
#5
Li Chen, Ruju Chen, Sherri Kemper, Min Cong, Hong You, David R Brigstock
The lack of approved therapies for hepatic fibrosis seriously limits medical management of patients with chronic liver disease. Since extracellular vesicles (EVs) function as conduits for intercellular molecular transfer, we investigated if EVs from healthy individuals have anti-fibrotic properties. Hepatic fibrogenesis or fibrosis in carbon tetrachloride (CCl4 )- or thioacetic acid-induced liver injury models in male or female mice were suppressed by serum EVs from normal mice (EVN) but not from fibrotic mice (EVF)...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29696079/profiling-plasma-extracellular-vesicle-by-pluronic-block-copolymer-based-enrichment-method-unveils-features-associated-with-breast-cancer-aggression-metastasis-and-invasion
#6
Zhenyu Zhong, Matthew Rosenow, Nick Xiao, David Spetzler
Extracellular vesicle (EV)-based liquid biopsies have been proposed to be a readily obtainable biological substrate recently for both profiling and diagnostics purposes. Development of a fast and reliable preparation protocol to enrich such small particles could accelerate the discovery of informative, disease-related biomarkers. Though multiple EV enrichment protocols are available, in terms of efficiency, reproducibility and simplicity, precipitation-based methods are most amenable to studies with large numbers of subjects...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29696078/newt-cells-secrete-extracellular-vesicles-with-therapeutic-bioactivity-in-mammalian-cardiomyocytes
#7
Ryan C Middleton, Russell G Rogers, Geoffrey De Couto, Eleni Tseliou, Kristin Luther, Ronald Holewinski, Daniel Soetkamp, Jennifer E Van Eyk, Travis J Antes, Eduardo Marbán
Newts can regenerate amputated limbs and cardiac tissue, unlike mammals which lack broad regenerative capacity. Several signaling pathways involved in cell proliferation, differentiation and survival during newt tissue regeneration have been elucidated, however the factors that coordinate signaling between cells, as well as the conservation of these factors in other animals, are not well defined. Here we report that media conditioned by newt limb explant cells (A1 cells) protect mammalian cardiomyocytes from oxidative stress-induced apoptosis...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29696077/investigation-of-procoagulant-activity-in-extracellular-vesicles-isolated-by-differential-ultracentrifugation
#8
Thøger Nielsen, Anne Flou Kristensen, Shona Pedersen, Gunna Christiansen, Søren Risom Kristensen
Tissue factor (TF) is the main initiator of coagulation and procoagulant phospholipids (PPL) are key components in promoting coagulation activity in blood. Both TF and PPL may be presented on the surface of extracellular vesicles (EVs), thus contributing to their procoagulant activity. These EVs may constitute a substantial part of pathological hypercoagulability that is responsible for triggering a higher risk of thrombosis in certain patients. The aim of this study was to describe a model system for the isolation of EVs required for investigating their effect on coagulation...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29696076/improved-resolution-in-extracellular-vesicle-populations-using-405-instead-of-488-nm-side-scatter
#9
Mark J McVey, Christopher M Spring, Wolfgang M Kuebler
Improvements in identification and assessment of extracellular vesicles (EVs) have fuelled a recent surge in EV publications investigating their roles as biomarkers and mediators of disease. Meaningful scientific comparisons are, however, hampered by difficulties in accurate, reproducible enumeration and characterization of EVs in biological fluids. High-sensitivity flow cytometry (FCM) is presently the most commonly applied strategy to assess EVs, yet its utility is limited by variant ability to resolve smaller EVs...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29696075/circulating-extracellular-vesicles-as-potential-biomarkers-in-chronic-fatigue-syndrome-myalgic-encephalomyelitis-an-exploratory-pilot-study
#10
Jesús Castro-Marrero, Esther Serrano-Pertierra, Myriam Oliveira-Rodríguez, Maria Cleofé Zaragozá, Alba Martínez-Martínez, María Del Carmen Blanco-López, José Alegre
Chronic Fatigue Syndrome (CFS), also known as Myalgic Encephalomyelitis (ME) is an acquired, complex and multisystem condition of unknown etiology, no established diagnostic lab tests and no universally FDA-approved drugs for treatment. CFS/ME is characterised by unexplicable disabling fatigue and is often also associated with numerous core symptoms. A growing body of evidence suggests that extracellular vesicles (EVs) play a role in cell-to-cell communication, and are involved in both physiological and pathological processes...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29696074/glycosylated-extracellular-vesicles-released-by-glioblastoma-cells-are-decorated-by-ccl18-allowing-for-cellular-uptake-via-chemokine-receptor-ccr8
#11
Jordi Berenguer, Tonny Lagerweij, Xi Wen Zhao, Sophie Dusoswa, Petra van der Stoop, Bart Westerman, Mark C de Gooijer, Marloes Zoetemelk, Anoek Zomer, Matheus H W Crommentuijn, Laurine E Wedekind, Àlan López-López, Alberta Giovanazzi, Marina Bruch-Oms, Ida H van der Meulen-Muileman, Rogier M Reijmers, Toin H van Kuppevelt, Juan-Jesús García-Vallejo, Yvette van Kooyk, Bakhos A Tannous, Pieter Wesseling, Danijela Koppers-Lalic, W Peter Vandertop, David P Noske, Victor W van Beusechem, Jacco van Rheenen, D Michiel Pegtel, Olaf van Tellingen, Thomas Wurdinger
Cancer cells release extracellular vesicles (EVs) that contain functional biomolecules such as RNA and proteins. EVs are transferred to recipient cancer cells and can promote tumour progression and therapy resistance. Through RNAi screening, we identified a novel EV uptake mechanism involving a triple interaction between the chemokine receptor CCR8 on the cells, glycans exposed on EVs and the soluble ligand CCL18. This ligand acts as bridging molecule, connecting EVs to cancer cells. We show that glioblastoma EVs promote cell proliferation and resistance to the alkylating agent temozolomide (TMZ)...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29535851/glycosylation-of-extracellular-vesicles-current-knowledge-tools-and-clinical-perspectives
#12
REVIEW
Charles Williams, Felix Royo, Oier Aizpurua-Olaizola, Raquel Pazos, Geert-Jan Boons, Niels-Christian Reichardt, Juan M Falcon-Perez
It is now acknowledged that extracellular vesicles (EVs) are important effectors in a vast number of biological processes through intercellular transfer of biomolecules. Increasing research efforts in the EV field have yielded an appreciation for the potential role of glycans in EV function. Indeed, recent reports show that the presence of glycoconjugates is involved in EV biogenesis, in cellular recognition and in the efficient uptake of EVs by recipient cells. It is clear that a full understanding of EV biology will require researchers to focus also on EV glycosylation through glycomics approaches...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29535850/scalable-cgmp-compatible-purification-of-extracellular-vesicles-carrying-bioactive-human-heterodimeric-il-15-lactadherin-complexes
#13
Dionysios C Watson, Bryant C Yung, Cristina Bergamaschi, Bhabadeb Chowdhury, Jenifer Bear, Dimitris Stellas, Aizea Morales-Kastresana, Jennifer C Jones, Barbara K Felber, Xiaoyuan Chen, George N Pavlakis
The development of extracellular vesicles (EV) for therapeutic applications is contingent upon the establishment of reproducible, scalable, and high-throughput methods for the production and purification of clinical grade EV. Methods including ultracentrifugation (U/C), ultrafiltration, immunoprecipitation, and size-exclusion chromatography (SEC) have been employed to isolate EV, each facing limitations such as efficiency, particle purity, lengthy processing time, and/or sample volume. We developed a cGMP-compatible method for the scalable production, concentration, and isolation of EV through a strategy involving bioreactor culture, tangential flow filtration (TFF), and preparative SEC...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29535849/extracellular-vesicles-as-a-platform-for-membrane-associated-therapeutic-protein-delivery
#14
REVIEW
Yoosoo Yang, Yeonsun Hong, Eunji Cho, Gi Beom Kim, In-San Kim
Membrane proteins are of great research interest, particularly because they are rich in targets for therapeutic application. The suitability of various membrane proteins as targets for therapeutic formulations, such as drugs or antibodies, has been studied in preclinical and clinical studies. For therapeutic application, however, a protein must be expressed and purified in as close to its native conformation as possible. This has proven difficult for membrane proteins, as their native conformation requires the association with an appropriate cellular membrane...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29511463/biocompatibility-of-highly-purified-bovine-milk-derived-extracellular-vesicles
#15
Masaharu Somiya, Yusuke Yoshioka, Takahiro Ochiya
Extracellular vesicles (EVs) deliver biologically active cargos from donor cells to recipient cells for intercellular communication. Since the existence of RNA cargo was discovered, EVs have been considered to be useful drug-delivery systems. Specifically, EVs from bovine milk (mEV) are one of the most promising platforms, since bovine milk is a scalable source of EVs for mass production. However, it is still difficult to isolate pure EVs from bovine milk owing to the complexity of raw materials. Furthermore, the biocompatibility and immunotoxicity of mEVs are still unclear...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29511462/proteome-profiling-of-extracellular-vesicles-captured-with-the-affinity-peptide-vn96-comparison-of-laemmli-and-trizol%C3%A2-protein-extraction-methods
#16
Andrew P Joy, D Craig Ayre, Ian C Chute, Annie-Pier Beauregard, Gabriel Wajnberg, Anirban Ghosh, Stephen M Lewis, Rodney J Ouellette, David A Barnett
Sample amount is often a limiting factor for multi-parametric analyses that encompass at least three areas of '-omics' research: genomics, transcriptomics and proteomics. Limited sample amounts are also an important consideration when these multi-parametric analyses are performed on extracellular vesicles (EVs), as the amount of EVs (and EV cargo) that can be isolated is often very low. It is well understood that a monophasic solution of phenol and guanidine isothiocyanate (i.e. TRIzol©) can simultaneously isolate DNA, RNA and proteins from biological samples; however, it is most commonly used for the extraction of RNA...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29511461/extracellular-vesicles-the-growth-as-diagnostics-and-therapeutics-a-survey
#17
REVIEW
Sabrina Roy, Fred H Hochberg, Pamela S Jones
This article aims to document the growth in extracellular vesicle (EV) research. Here, we report the growth in EV-related studies, patents, and grants as well as emerging companies with major intent on exosomes. Four different databases were utilized for electronic searches of published literature: two general databases - Scopus/Elsevier and Web of Science (WoS), as well as two specialized US government databases - the USA Patent and Trademark Office and National Institutes of Health (NIH) of the Department of Health and Human Services...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29511460/immunoaffinity-based-isolation-of-melanoma-cell-derived-exosomes-from-plasma-of-patients-with-melanoma
#18
Priyanka Sharma, Sonja Ludwig, Laurent Muller, Chang Sook Hong, John M Kirkwood, Soldano Ferrone, Theresa L Whiteside
Tumour-derived exosomes (TEX) are a subset of extracellular vesicles (EVs) present in body fluids of patients with cancer. The role of this exosome subset in melanoma progression has been of interest ever since ex vivo studies of exosomes produced by melanoma cell lines were shown to suppress anti-melanoma immune responses. To study the impact of melanoma-derived exosomes (MTEX) present in patients' plasma on melanoma progression, isolation of MTEX from total plasma exosomes is necessary. We have developed an immunoaffinity-based method for MTEX capture from plasma of melanoma patients...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29435202/podocyte-derived-microparticles-promote-proximal-tubule-fibrotic-signaling-via-p38-mapk-and-cd36
#19
Mercedes N Munkonda, Shareef Akbari, Chloe Landry, Suzy Sun, Fengxia Xiao, Maddison Turner, Chet E Holterman, Rania Nasrallah, Richard L Hébert, Christopher R J Kennedy, Dylan Burger
Tubulointerstitial fibrosis is a hallmark of advanced diabetic kidney disease that is linked to a decline in renal function, however the pathogenic mechanisms are poorly understood. Microparticles (MPs) are 100-1000 nm vesicles shed from injured cells that are implicated in intercellular signalling. Our lab recently observed the formation of MPs from podocytes and their release into urine of animal models of type 1 and 2 diabetes and in humans with type 1 diabetes. The purpose of the present study was to examine the role of podocyte MPs in tubular epithelial cell fibrotic responses...
2018: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/29410780/characterization-of-trichuris-muris-secreted-proteins-and-extracellular-vesicles-provides-new-insights-into-host-parasite-communication
#20
Ramon M Eichenberger, Md Hasanuzzaman Talukder, Matthew A Field, Phurpa Wangchuk, Paul Giacomin, Alex Loukas, Javier Sotillo
Whipworms are parasitic nematodes that live in the gut of more than 500 million people worldwide. Owing to the difficulty in obtaining parasite material, the mouse whipworm Trichuris muris has been extensively used as a model to study human whipworm infections. These nematodes secrete a multitude of compounds that interact with host tissues where they orchestrate a parasitic existence. Herein we provide the first comprehensive characterization of the excretory/secretory products of T. muris . We identify 148 proteins secreted by T...
2018: Journal of Extracellular Vesicles
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