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GMS Hygiene and Infection Control

Johanna Stranzinger, Jutta Kindel, Melanie Henning, Dana Wendeler, Albert Nienhaus
Background: Staff in children's hospitals may run an increased risk of cytomegalovirus (CMV) contact infection leading to a congenital CMV fetopathy during pregnancy. The main risk factor is close contact with inapparent carriers of CMV among infants (<3 years). We therefore examined CMV seroprevalence (SP) and possible risk factors for CMV infection among staff at a children's hospital. Method: In 2014, staff at a metropolitan children's hospital were offered a CMV antibody test in the context of occupational health screening...
2016: GMS Hygiene and Infection Control
Hamed Ebrahimzadeh Leylabadlo, Elham Zeinalzadeh, Najibeh Asl Rahnemaii Akbari, Hossein Samadi Kafil
Infection is a serious complication after implantation of total knee-prostheses. However, fungal infection is rarely found in periprosthetic joints, and in most reports, the infecting organism is a Candida species. This is a case report of infection after left knee total arthroplasty caused by Malassezia species. The patient is still undergoing antifungal therapy with voriconazole and is still being followed-up. To the authors' knowledge, the present case is the first report of Malassezia species in a patient after total knee arthroplasty...
2016: GMS Hygiene and Infection Control
Seyed Masoud Mousavi, Mona Nasaj, Seyed Mostafa Hosseini, Mohammad Reza Arabestani
Aim: The aims of the present study were to determine the antibiotic susceptibility profils with particular emphasis on susceptible or resistant strains to macrolides and lincosamids antibiotics and to determine possible antibiotic resistance mechanisms occurring in group B streptococci (GBS) strains using PCR assay and disk diffusion method. Methods: A total of 62 clinical GBS strains were investigated. Antibacterial susceptibility testing was performed using the disk diffusion method and inducible resistance test for clindamycin by standard double disk diffusion or D-zone test for all isolates to differentiate macrolide resistance phenotype (M), constitutive macrolide-lincosamide-streptogramin B phenotype (cMLSB) and induced macrolide-lincosamide-streptogramin B phenotype (iMLSB)...
2016: GMS Hygiene and Infection Control
Anne Lührmann, Rutger Matthes, Axel Kramer
AIM: The antimicrobial activity of cold atmospheric pressure plasma (CAP), also called tissue tolerable plasma (TTP), could be a promising option to eradicate methicillin-sensitive as well as methicillin-resistant Staphylococcus aureus strains, which often colonize chronic wounds. Currently, the influence of CAP on the susceptibility of S. aureus to antibiotics is scarcely known, but could be important for treatment of wounds. Therefore, the aim of this study was to investigate whether CAP has an impact on the susceptibility of different S...
2016: GMS Hygiene and Infection Control
Ursel Heudorf, Sabine Albert-Braun, Klaus-Peter Hunfeld, Franz-Ulrich Birne, Jörg Schulze, Klaus Strobel, Knut Petscheleit, Volkhard A J Kempf, Christian Brandt
INTRODUCTION: The refugee crisis is a great challenge to the social and healthcare system in European countries, especially in Germany. An abundance of data has been published on the refugees' health problems (infections as well as physical diseases and psychiatric problems) and their prevention (i.e., sanitary and vaccination programs). However, data on prevalences of multidrug-resistant organisms (MDRO) in refugees are scarce, although it is known that most refugees are from or travelled through countries with high prevalences of MDRO...
2016: GMS Hygiene and Infection Control
Ursel Heudorf, Stefanie Gasteyer, Maria Müller, Yvonne Samoiski, Nicole Serra, Tim Westphal
INTRODUCTION: Urinary tract infections range among the most frequent infections not only in hospital patients but also in residents of long-term care facilities for the elderly. Urinary catheters are the greatest risk factor for urinary tract infections. In the guidance paper on the "prevention of infections in nursing homes" (2005) as well as in the updated recommendations for the "prevention and control of catheter-associated urinary tract infections" (2015), the Commission for Hospital Hygiene and Infection Prevention (KRINKO) has recommended adequate preventive measures...
2016: GMS Hygiene and Infection Control
Ursel Heudorf, Miriam Grünewald, Ulla Otto
AIM: The Commission for Hospital Hygiene and Infection Prevention (KRINKO) updated the recommendations for the prevention of catheter-associated urinary tract infections in 2015. This article will describe the implementation of these recommendations in Frankfurt's hospitals in autumn, 2015. MATERIAL AND METHODS: In two non-ICU wards of each of Frankfurt's 17 hospitals, inspections were performed using a checklist based on the new KRINKO recommendations. In one large hospital, a total of 5 wards were inspected...
2016: GMS Hygiene and Infection Control
Gabriella Eva Cristina Gall, Stephan Lautenschlager, Homayoun C Bagheri
Syphilis is considered as one of the most devastating sexually transmitted diseases in human history. Based on historical records, the "Böse Blattern" (German for "Evil Pocks") spread through Europe after 1495 and shared symptoms with what we know today as syphilis. Many cities took measures to protect their population. Here, transliterations of archival documents from the 15(th) and 16(th) century (provided in the appendix) are used to trace the steps taken by the governing authorities in Zurich to deal with this emerging infectious disease...
2016: GMS Hygiene and Infection Control
Fahimeh Nourbakhsh, Amirmorteza Ebrahimzadeh Namvar
Staphylococcus aureus is one of the Gram-positive pathogens causing a wide range of nosocomial infections. The present study investigates genotypic and phenotypic aspects involved in biofilm formation in methicillin-resistant Staphylococcus aureus strains isolated from nosocomial infections in Isfahan. A total of 110 S. aureus strains were collected from three major hospitals in Isfahan, the center of Iran. The antibiotic resistance pattern, phenotypes, and biofilm formation genes were studied using Congo red agar (CRA) and multiplex PCR (M-PCR)...
2016: GMS Hygiene and Infection Control
Annick Lefebvre, Catherine Quantin, Philippe Vanhems, Jean-Christophe Lucet, Xavier Bertrand, Karine Astruc, Pascal Chavanet, Ludwig S Aho-Glélé
AIM: We aimed to study the impact of new water systems, which were less contaminated with P. aeruginosa, on the incidence of healthcare-associated P. aeruginosa cases (colonizations or infections) in care units that moved to a different building between 2005 and 2014. METHODS: Generalized Estimated Equations were used to compare the incidence of P. aeruginosa healthcare-associated cases according to the building. RESULTS: Twenty-nine units moved during the study period and 2,759 cases occurred in these units...
2016: GMS Hygiene and Infection Control
Arne Simon, Rhoikos Furtwängler, Norbert Graf, Hans Jürgen Laws, Sebastian Voigt, Brar Piening, Christine Geffers, Philipp Agyeman, Roland A Ammann
Pediatric patients receiving conventional chemotherapy for malignant disease face an increased risk of bloodstream infection (BSI). Since BSI may represent an acute life-threatening event in patients with profound immunosuppression, and show further negative impact on quality of life and anticancer treatment, the prevention of BSI is of paramount importance to improve and guarantee patients' safety during intensive treatment. The great majority of all pediatric cancer patients (about 85%) have a long-term central venous access catheter in use (type Broviac or Port; CVAD)...
2016: GMS Hygiene and Infection Control
Ursel Heudorf, Barbara Büttner, Anja M Hauri, Petra Heinmüller, Klaus-Peter Hunfeld, Martin Kaase, Niels Kleinkauf, Sabine Albert-Braun, Rolf Tessmann, Volkhard A J Kempf
BACKGROUND: Multidrug-resistant Gram-negative bacteria (MRGN) and the infections they cause are a serious threat and a challenge to the healthcare system. This particularly applies to carbapenem-resistant Gram-negative bacteria (CRGN). Currently, the introduction of a nationwide mandatory notification system for CRGN in Germany is under consideration. Against this background, this paper presents an analysis of the mandatory reporting system for CRGN in effect since November 2011 in the federal state of Hesse (Germany)...
2016: GMS Hygiene and Infection Control
Johanna Stranzinger, Agnessa Kozak, Benjamin Schilgen, Diana Paris, Thomas Nießen, Lutz Schmidt, Andreas Wille, Norbert L Wagner, Albert Nienhaus
BACKGROUND: Close contact with asymptomatic children younger than three years is a risk factor for a primary cytomegalovirus (CMV) infection. In pregnant women, such primary infection increases the risk of CMV-induced feto- or embryopathy. Daycare providers have therefore implemented working restrictions for pregnant daycare workers (DCWs) in accordance with legislation and guidelines for maternity protection. However, little is known about the infection risk for DCWs. We therefore compared the prevalence of CMV antibodies of pregnant DCWs to that of female blood donors (BDs)...
2016: GMS Hygiene and Infection Control
Reyad R Shawish, Naser A Al-Humam
Food-borne pathogens are of high concern for public health and food safety. Staphylococcus aureus food poisoning is one of the most economically devastating types of food poisoning globally. The purpose of this study was to detect staphylococcal classical enterotoxins (SEs) in processed beef from Kingdom of Saudi Arabia (KSA) and Egypt. In the present investigation a total of 250 random processed meat samples (50 each of minced meat, beef burger, beef sausage, beef kofta and beef luncheon) were collected from different super markets in the study area...
2016: GMS Hygiene and Infection Control
Birgul Kacmaz, Serdar Gul
AIM: Paper is used for various purposes in hospitals. Generally, there are two different types of paper, which are commonly used in our facility: wood-free paper, and paper containing wood. We compared the recoverable proportion of methicillin-resistant Staphylococcus aureus (MRSA; ATCC 43300) from the surface of such papers. METHOD: The papers were divided into two groups: Group 1: wood-free paper; Group 2: paper containing wood. The papers were contaminated in a standardized procedure with 0...
2016: GMS Hygiene and Infection Control
Ursel Heudorf, Maria Karathana, Bernhard Krackhardt, Meike Huber, Peter Raupp, Christian Zinn
In 2015, most of the refugees arriving in Germany originated from countries with poor hygienic and sanitary conditions. Stool samples of 1,230 minor refugees unaccompanied by adults were investigated for possible parasites. Giardia lamblia was by far the most frequently detected parasite (n=165); all other parasites were considerably less frequent and encountered in the following order: Hymenolepis nana (n=23), Entamoeba histolytica (n=17), Trichuris trichiura (n=8), and Blastocystis hominis (n=1). Ascaris lumbricoides was not detected among any of the screened refugees...
2016: GMS Hygiene and Infection Control
Somayeh Azimi, Hossein Samadi Kafil, Hossein Bannazadeh Baghi, Saeed Shokrian, Khadijeh Najaf, Mohammad Asgharzadeh, Mehdi Yousefi, Firooz Shahrivar, Mohammad Aghazadeh
BACKGROUND: Pseudomonas aeruginosa, as Gram-negative rod bacilli, has an important role in human infection. In the present study we aimed to investigate the presence of exo genes and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran. MATERIAL AND METHODS: 160 isolates of P. aeruginosa were collected and identified by biochemical tests and were characterized for antibiotic resistance. Biofilm production was evaluated by microtiter plate assay and the presence of exo genes was evaluated by allele-specific PCR (polymerase chain reaction)...
2016: GMS Hygiene and Infection Control
Rüdiger Külpmann, Bärbel Christiansen, Axel Kramer, Peter Lüderitz, Frank-Albert Pitten, Frank Wille, Klaus-Dieter Zastrow, Friederike Lemm, Regina Sommer, Milo Halabi
Since the publication of the first "Hospital Hygiene Guideline for the implementation and operation of air conditioning systems (HVAC systems) in hospitals" ( in 2002, it was necessary due to the increase in knowledge, new regulations, improved air-conditioning systems and advanced test methods to revise the guideline. Based on the description of the basic features of ventilation concepts, its hygienic test and the usage-based requirements for ventilation, the DGKH section "Ventilation and air conditioning technology" attempts to provide answers for the major air quality issues in the planning, design and the hygienically safe operation of HVAC systems in rooms of health care...
2016: GMS Hygiene and Infection Control
Matthias Leonhard, Ojan Assadian, Michaela Zumtobel, Berit Schneider-Stickler
BACKGROUND: Manufacturers' recommendations on cleaning of tracheostomy tubes focus on general warning information and non-specific manual cleaning procedures. The aim of this experimental study was to evaluate different reprocessing methods and to determine the mechanical integrity and functionality of tracheostomy tubes following reprocessing. METHODS: Sixteen cuffed or un-cuffed tracheostomy tubes obtained from hospital in-patients were reprocessed using one of the following reprocessing methods: a) manual brushing and rinsing with tap water, b) manual brushing followed by disinfection with a glutaraldehyde solution, c) manual brushing followed machine-based cleaning in a dishwasher, and d) manual brushing followed by ultrasound cleaning in a commercially available ultrasound device...
2016: GMS Hygiene and Infection Control
Axel Kramer, Ojan Assadian
While the requirements for single-use gloves for staff protection are clearly defined, the conventional medical differentiation between "sterile surgical gloves" used during surgical procedures and "single-use medical gloves" used in non-sterile medical areas does not adequately define the different requirements in these two areas of use. Sterilization of single-use medical gloves is not performed if sterility is not required; thus, another terminology must be found to identify the safety quality of non-sterile single-use medical gloves...
2016: GMS Hygiene and Infection Control
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