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African Journal of Infectious Diseases

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https://www.readbyqxmd.com/read/29619446/isolation-and-identification-of-brucella-suis-in-pigs-as-zoonotic-disease-in-endemic-areas-of-east-java-indonesia
#1
Emy Koestanti S, Wiwik Misaco, Sri Chusniati, Lilik Maslachah
Background: Brucellosis in pigs at East Java Indonesia has not only cause great economic losses due to a decrease in productivity of livestock but also are zoonotic. Infection on free brucelosis pigs were initially begun with the infected pigs both male and female, or the use of superior male pigs together. The elimination of the disease either on a group or population is considered as the most effective way to prevent the spread of the disease in pigs. Prevention efforts mainly addressed to vaccination, sanitary maintenace and government policy...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619445/in-silico-screening-and-biological-evaluation-of-the-compounds-of-justicia-gendarussa-leaves-extract-as-interferon-gamma-inducer-a-study-of-anti-human-immunodeficiency-virus-hiv-development
#2
Restry Sinansari, Bambang Prajogo Ew, Prihartini Widiyanti
Background: Justicia gendarussa Burm f. (Achanthaceae) has been known as traditional medicine in Indonesia. It contains of flavonoids and alkaloids. This study was conducted to evaluate the effect of J. gendarussa on the profile of IFN-γ on mice (Mus musculus ). Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on the J. gendarussa leaves against IFN-γ receptor. It is expected that this research will provide scientific information on the development of J...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619444/effect-of-varying-incubation-periods-on-cytotoxicity-and-virucidal-activities-of-justicia-gendarussa-burm-f-leaf-extract-on-hiv-infected-molt-4-cells
#3
Prihartini Widiyanti, Bambang Prajogo, Agustinus Widodo
Backgrounds: Justicia gendarussa Burm.f. has an anti-HIV activity. This study was conducted to evaluate the effects of incubation periods on the cytotoxicity and virucidal activities of the J. gendarussa leaves extract on MOLT-4 cells. Materials and Methods: The cytotoxicity assay was evaluated by using the WST-1 test with incubation periods of 3 days and 5 days. The virucidal activity test was determined by measuring the inhibitory activities on the syncytium formation...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619443/cloning-and-expression-of-mce1a-gene-from-mycobacterium-tuberculosis-beijing-and-h37rv-strain-for-vaccine-candidate-development
#4
Desi Indriarini, Andriansjah Rukmana, Andi Yasmon
Background: Tuberculosis remains the leading cause of death in the world, especially wherever poverty, malnutrition and poor housing prevail. Mycobacterium tuberculosis Beijing strain is the most common strain that causes tuberculosis in Indonesia. The wide spread of tuberculosis has been further aggravated by HIV-AIDS and drug resistance. Unfortunately, Bacille Calmette-Guerin (BCG) as the current vaccine has different protection function and efficacy. According to function analysis, mce1A gene was predicted to have a role in host invasion and survival of Mycobacterium tuberculosis in human macrophages...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619442/comparison-of-multiplex-single-round-pcr-and-microscopy-in-diagnosis-of-amoebiasis
#5
Sri-Hidajati Bs, Sukmawati Basuki, Suhintam Pusarawati, Kusmartisnawati, Lynda Rossyanti, Sri Wijayanti Sulistyowati, Dwi Peni Kartikasari, Heny Arwati, Indah Tantular, Alpha Fardah, Andy Darma, Retno Handajani, Subijanto Marto Soedarmo
Background: Amoebiasis, the cause of dysentery and extra-intestinal abscesses, now becomes second fatal parasitic disease in the world. As routine microscopic diagnosis cannot differentiate causative Entamoeba histolytica from non-pathogenic E. dispar and E. moshkovskii , better diagnosis has to be searched. Materials and Methods: Multiplex single round PCR was tested and compared with results of microscopy of wet preparation on 30 samples of diarrheic stools and extra intestinal lesions from amoebiasis suspected patients...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619441/a-new-copper-ii-imidazole-derivative-effectively-inhibits-replication-of-denv-2-in-vero-cell
#6
Teguh Hari Sucipto, Siti Churrotin, Harsasi Setyawati, Fahimah Martak, Kris Cahyo Mulyatno, Ilham Harlan Amarullah, Tomohiro Kotaki, Masanori Kameoka, Subagyo Yotopranoto, And Soegeng Soegijanto
Background: Dengue is a kind of infectious disease that was distributed in the tropical and sub-tropical areas. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Therefore, finding the effective compound against dengue virus (DENV) replication is very important. Among the complex compounds, copper(II)-imidazole derivatives are of interest because of their biological and medicinal benefits. Materials and Methods: In the present study, antiviral activity of [Cu(2,4,5-triphenylimidazole)2 ]n , was evaluated against different stages of dengue virus type 2 (DENV-2) replication in Vero cell using focus forming unit reduction assay and quantitative ELISA...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619440/determination-of-effective-dose-of-antimalarial-from-cassia-spectabilis-leaf-ethanol-extract-in-plasmodium-berghei-infected-mice
#7
Wiwied Ekasari, Tutik Sri Wahyuni, Heny Arwaty, Nindya T Putri
Background: The preliminary study on antimalarial activity of the ethanol extract of Cassia spectabilis leaves against Plasmodium berghei has been carried out by in vivo experiment. It was demonstrated that ethanol extract of C. spectabilis leaves could inhibit growth of rodent malaria parasite P. berghei by 59.29 % (at a dose of 100 mg/kg bodyweight). However, further investigation is required to determine an effective dose of the administered extract for a higher inhibitory effect and increasing effectiveness of the extract...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619439/evaluation-of-the-antigenicity-and-immunogenicity-of-eimeria-tenella-by-reproductive-index-and-histopathological-changes-of-cecal-coccidiosis-virulent-live-vaccine-in-broiler-chickens
#8
Endang Suprihati, Muchammad Yunus
Background: The development of vaccine to control coccidiosis caused by Eimeria tenella ( E. tenella ) in chickens is intensifying because of the increasing threat of drug resistance to anticoccidial agents. It is important, therefore, to develop a reliable standard method for the assessment of vaccine afficacy particularly antigenicity and immunogenicity become crucial. Evaluation of E. tenella antigenicity and immunogenicity to some low doses can be reflected by reproductive index and histopathological changes...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619438/comparison-of-anti-bacterial-efficacy-of-photodynamic-therapy-and-doxycycline-on-aggregatibacter-actinomycetemcomitans
#9
Ernie Maduratna Setiawatie, Vina Puji Lestari, Suryani Dyah Astuti
Background: Aggregatibacter actinomycetemcomitans ( A.actinomycetemcomitans ) is an anaerobic bacterium has been frequently associated with aggressive periodontitis. Photodynamic therapy (PDT) is a medical treatment to prevent infection progression that utilizes light to activate a photosensitizing agent. Doxycycline is an antibacterial having photosensitivity. This study aimed to evaluate potential doxycycline as an antibacterial and photosensitizer combine PDT against A.actinomycetemcomitans bacteria...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619437/risk-factors-of-vulvovaginal-candidiasis-in-dermato-venereology-outpatients-clinic-of-soetomo-general-hospital-surabaya-indonesia
#10
Dharin Serebrina Arfiputri, Afif Nurul Hidayati, Samsriyaningsih Handayani, Evy Ervianti
Background: Vulvovaginal Candidiasis (VVC) is one-third of vaginitis case. About 75% of women will have at least one episode of VVC. Complication of VVC can be unfavorable to impact the patient's quality of life. Knowing its risk factors can prevent someone suffering from pathological VVC and its sequelae. The purpose of this study was to identify risk factors of VVC. Materials and Methods: A descriptive retrospective study was conducted by total sampling to 213 medical records of VVC patients from 869 fluor albus patients in Sexually Transmitted Infection (STI) Division, Dermatology and Venereology Outpatient Clinic in Dr...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619436/concomitant-sexually-transmitted-diseases-in-patients-with-diagnosed-hiv-aids-a-retrospective-study
#11
Densy Violina Harnanti, Afif Nurul Hidayati, Muhammad Miftahussurur
Background: Human immunodeficiency virus (HIV) is a virus that causes Acquired Immunodeficiency Syndrome (AIDS) which weaken the human immune system and thus increasing the incidence of sexually transmitted infections (STIs) and vice versa. Materials and Methods: A retrospective study of STIs in HIV/AIDS patients in Unit Perawatan Intermediate Penyakit Infeksi (UPIPI) Dr. Soetomo General Hospital Surabaya was conducted from January 1st, 2013 to December 31st, 2014...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619435/seroprevalence-and-risk-factor-of-toxoplasmosis-in-schizophrenia-patients-referred-to-grhasia-psychiatric-hospital-yogyakarta-indonesia
#12
Nina Difla Muflikhah, Supargiyono, Wayan Tunas Artama
Background: Toxoplasmosis is an infectious disease caused by protozoan parasite called Toxoplasma gondii . Toxoplasma gondii is an intracellular protozoan parasite belong to phylum Apicomplexa, is an obligate parasite in mammals. The active proliferating trophozoites or tachyzoites are usually seen in the acute stage of infection, while the resting bradyzoites formed tissue cysts are primary found in muscle and brain. Human infection occurs mainly by ingesting food or water contaminated with oocyst or eating an undercook meat containing tissue cyst...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619434/addition-of-anti-toxoplasma-gondii-membrane-immunoglobulin-y-to-reduce-necrotic-index-in-mice-s-liver
#13
Heni Puspitasari, Lucia T Suwanti, Mufasirin
Background: The study aimes to determine the effect of administering anti- T. gondii membrane IgY against liver damage (Necrotic index) and the effectiveness of the antibody's delivery time. Materials and Methods: This research was a laboratory experiment with five treatments and five replicantions. Each treatment used female mice ( Mus musculus ) as animal models. The treatment groups consisted of a P0 group (not infected), P1 group (infected), P2 group (anti- T...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619433/increased-apoptosis-skull-of-pups-born-to-toxoplasma-gondii-infected-mice-associated-with-increased-expression-of-interferon-gamma-but-not-tumor-necrosis-factor-alfa
#14
Lucia Tri Suwanti, Mufasirin
Bacground: Toxoplasma gondii is an intracellular obligate protozoan parasite that infects most warm-blooded animals including humans. It can cause congenital infection with clinical symptoms ranging from mild to severe including microcephaly. At the cellular level, infection T. gondii causes apoptosis in some tissues and it is induced by proinflammatory cytokines, such as IFN-γ and TNF-α. The purpose of this study is to determine the role of proinflammatory cytokines (TNF-α and IFN-γ) to apoptosis skull of newborn from T...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619432/antibacterial-activity-of-dracontomelon-dao-extracts-on-methicillin-resistant-s-aureus-mrsa-and-e-coli-multiple-drug-resistance-mdr
#15
Yuniati Yuniati, Nurul Hasanah, Sjarif Ismail, Silvia Anitasari, Swandari Paramita
Background: Staphylococcus aureus , methicillin-resistant and Escherichia coli , multidrug-resistant included in the list of antibiotic-resistant priority pathogens from WHO. As multidrug-resistant bacteria problem is increasing, it is necessary to probe new sources for identifying antimicrobial compounds. Medicinal plants represent a rich source of antimicrobial agents. One of the potential plants for further examined as antibacterial is Dracontomelon dao (Blanco) Merr. & Rolfe. The present study designed to find the antibacterial activity of D...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619431/the-role-of-psychological-well-being-in-boosting-immune-response-an-optimal-effort-for-tackling-infection
#16
Abdurachman, Netty Herawati
Background: Many attempts have been made to improve human body resistance towards infection. These attempts were exemplified by promotion of hygiene and the use of antiseptic ingredients, pharmaceutical compounds, and vaccines. To prove an aspect which intentionally less explored was attempt to boosting the host immune responses by creating a psychological balance, which was important aspect of psychological well-being. Material and Method: These article reviews searched and compiled using database Google Scholar, from 1995 to latest...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619430/cd4-and-cd8-t-cells-expressing-interferon-gamma-in-active-pulmonary-tuberculosis-patients
#17
Betty Agustina Tambunan, Hery Priyanto, Jusak Nugraha, Soedarsono
Background: Tuberculosis (TB) is a global health problem. Immune response through CD4+ and CD8+ T cells is needed to produce Interferon gamma (IFN-γ), a cytokine eliminate Mycobacterium tuberculosis . We aimed to compare the cellular immune response based on the percentage of CD4+ and CD8+ T cells expressing interferon gamma in active pulmonary tuberculosis patients before and after 2 months of tuberculosis treatment. Methods: It is a longitudinal cohort study included 12 patients with new active pulmonary TB of the Pulmonary Hospital, Surabaya...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619429/-acanthamoeba-sp-s-11-phagocytotic-activity-on-mycobacterium-leprae-in-different-nutrient-conditions
#18
Sepling Paling, Ratna Wahyuni, Ni'matuzahroh, Dwi Winarni, Iswahyudi, Linda Astari, Dinar Adriaty, Indropo Agusni, Shinzo Izumi
Background: Mycobacterium leprae ( M. leprae ) is a pathogenic bacterium that causes leprosy. The presence of M. leprae in the environment is supported by microorganisms that act as the new host for M. leprae . Acanthamoeba 's potential to be a host of M. leprae in the environment. Acanthamoeba sp. is Free Living Amoeba (FLA) that classified as holozoic, saprophytic, and saprozoic. The existence of nutrients in the environment influence Acanthamoeba ability to phagocytosis or pinocytosis...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619428/antiviral-activity-of-justicia-gendarussa-burm-f-leaves-against-hiv-infected-mt-4-cells
#19
Agustinus Widodo, Prihartini Widiyanti, Bambang Prajogo
Backgrounds: Justicia gendarussa Burm.f. has been known to have anti-HIV activity. This study was conducted to evaluate the effect of incubation time on the antiviral activity of the J. gendarussa leaves extract on HIV-infected MT-4 cells in vitro . Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on the J. gendarussa leaves against HIV-1 reverse transcriptase receptor. It is expected that this research will provide scientific information on the development of J...
2018: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29619427/determination-of-environmental-factors-affecting-dengue-incidence-in-sleman-district-yogyakarta-indonesia
#20
Tri Wulandari Kesetyaningsih, Sri Andarini, Sudarto, Henny Pramoedyo
Background: Dengue is a disease related to the environment that spreads rapidly. Prevention movement is considered ineffective; therefore, a more efficient early warning system is required. It is required strongly correlated variables to as predictor in early warning system. This study aims to identify the environmental conditions associated with dengue. Materials and methods: This ecological study was conducted on five sub-districts selected based on the trend of the incidence...
2018: African Journal of Infectious Diseases
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