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Yoshifumi Oshima, Soyeon Lee, Kunio Takayanagi
The detection of lithium ions is required for characterization of lithium ion batteries, since the movement of lithium ions in the battery is one of the key ways to improve the performance. Annular bright field (ABF) imaging enables us to visualize individual lithium atomic columns simultaneously with heavy elements. Furthermore, it has been found that the number of lithium ions at the column is countable when the specimen is thin. These results suggest that movement of lithium ions in the material can be observed by taking consecutive ABF images during operation or in situ ABF observation...
October 14, 2016: Microscopy
Kazuo Yamamoto, Yasutoshi Iriyama, Tsukasa Hirayama
All-solid-state Li-ion batteries having incombustible solid electrolytes are promising energy storage devices because they have significant advantages in terms of safety, lifetime and energy density. Electrochemical reactions, namely, Li-ion insertion/extraction reactions, commonly occur around the nanometer-scale interfaces between the electrodes and solid electrolytes. Thus, transmission electron microscopy (TEM) is an appropriate technique to directly observe such reactions, providing important information for understanding the fundamental solid-state electrochemistry and improving battery performance...
October 12, 2016: Microscopy
Shunsuke Muto, Kazuyoshi Tatsumi
Advancements in the field of renewable energy resources have led to a growing demand for the analysis of light elements at the nanometer scale. Detection of lithium is one of the key issues to be resolved for providing guiding principles for the synthesis of cathode active materials, and degradation analysis after repeated use of those materials. We have reviewed the different techniques currently used for the characterization of light elements such as high-resolution transmission electron microscopy, scanning transmission electron microscopy (STEM) and electron energy-loss spectroscopy (EELS)...
September 21, 2016: Microscopy
Shuji Kitahara, Junzo Desaki, Asuka Yoshii, Aya Matsui, Shunichi Morikawa, Taichi Ezaki
Capillary networks demonstrate structural changes during maturation, aging, vascular disease, and cancer. Their morphological structure and function have an important influence on each other. Understanding the process of morphological vascular changes in the capillary network with advancing age may help overcome fatal vascular diseases. Aging-related structural changes of the capillary segments may accompany degeneration and regeneration of muscle fibers and serve to remodel the capillary network as a means of adapting to the changing environment...
September 21, 2016: Microscopy
Scott David Findlay, Rong Huang, Ryo Ishikawa, Naoya Shibata, Yuichi Ikuhara
Annular bright field (ABF) scanning transmission electron microscopy has proven able to directly image lithium columns within crystalline environments, offering much insight into the structure and properties of lithium-ion battery materials. We summarize the image formation mechanisms underpinning ABF imaging, review the experimental application of this technique to imaging lithium in materials and overview the conditions that help maximize the visibility of lithium columns.
September 21, 2016: Microscopy
Van Vuong Hoang, Youngji Cho, Jung Ho Yoo, Soon-Ku Hong, Yong Ho Choi, Sungha Choi, Wooduck Jung, Chang Kyu Jeong, Jun-Mo Yang
Recent years have seen a great deal of progress in the development of transmission electron microscopy-based techniques for strain measurement. Dark-field electron holography (DFEH) is a new technique offering configuration of the off-axis principle. Using this technique with medium magnification (Holo-M), we carried out strain measurements in nanoscale-triangular SiGe/(001) Si with (004), (2(-)20) and ((-)111) diffraction spots. The reconstruction of holograms and interpretation of strain maps in term of strain precision were discussed and the strain distributions in the SiGe/(001) Si patterns were visualized...
September 8, 2016: Microscopy
Hiroshi Nakajima, Atsuhiro Kotani, Ken Harada, Yui Ishii, Shigeo Mori
We report a method for acquiring Foucault images and small-angle electron diffraction patterns in external magnetic fields using a conventional transmission electron microscope without any modification. In the electron optical system that we have constructed, external magnetic fields parallel to the optical axis can be controlled using the objective lens pole piece under weak excitation conditions in the Foucault mode and the diffraction mode. We observe two ferromagnetic perovskite-type manganese oxides, La0...
September 2, 2016: Microscopy
Ron Benyair, Gerardo Z Lederkremer
We found that a localization artifact can arise from common immunofluorescence methods. Specifically, cell fixation and permeabilization can cause mislocalization of a type II membrane-bound protein, ER mannosidase I, from its native localization in vesicles to the Golgi complex. Live cell microscopy and interestingly also mild cell fixation with paraformaldehyde without membrane permeabilization do not present this artifact.
September 1, 2016: Microscopy
Masaki Makihara, Takashi Watanabe, Eiji Usukura, Kozo Kaibuchi, Akihiro Narita, Nobuo Tanaka, Jiro Usukura
An improved unroofing method consisting of tearing off the cell membrane using an adhesive electron microscopy (EM) grid instead of vitreous ice sectioning (cryo-sectioning) has enabled us to panoramically view the membrane cytoskeleton in its native state with extremely high contrast. Grids pre-treated with Alcian blue were placed on cells, and a portion of the dorsal plasma membrane was transferred onto the grid, which was then floated in buffer solution. These membrane fragments contained sufficient cytoskeleton and were of suitable thickness for observation by cryo-EM...
September 1, 2016: Microscopy
Kazutoshi Inoue, Mitsuhiro Saito, Chunlin Chen, Motoko Kotani, Yuichi Ikuhara
A collaborative work between mathematics and atom-resolved scanning transmission electron microscopy (STEM) has been conducted. The grain boundary in a bicrystal of a simple rock-salt oxide can show a complicated arrangement of structural units, which can be well predicted by an algorithm utilizing the Farey sequence. The estimated arrangements had a nice agreement with those observed by STEM in atomic-scale up to several tens of nanometers.
September 1, 2016: Microscopy
Yunjie Chang, Yumei Wang, Yanxiang Cui, Binghui Ge
Transmission cross-coefficient theory and pseudo-weak-phase object approximation theory were combined to investigate the non-linear imaging in high-resolution transmission electron microscopy (HRTEM). The analytical expressions of linear and non-linear imaging components in diffractogram were obtained and changes of linear and non-linear components over sample thickness were analyzed. Moreover, the linear and non-linear components are found to be an odd and even-function of the defocus and Cs, respectively...
September 1, 2016: Microscopy
(no author information available yet)
No abstract text is available yet for this article.
October 2016: Microscopy
Katsumi Ura
I investigated the tilting and movement of the objective lens of a 3D electron microscope electrically as an extension of the moving-objective lens concept. The electric or magnetic potential along the tilted optical axis is analytically expressed by a multipole potential expansion about the fixed central axis. The field distributions for axially symmetric dipole and quadrupole components are numerically shown, where the optical axis of a bell-shaped magnetic lens is tilted around the lens center by up to 60°...
October 2016: Microscopy
Toshihiro Kogure, Noriko Yamaguchi, Hiroyo Segawa, Hiroki Mukai, Satoko Motai, Kotone Akiyama-Hasegawa, Masanori Mitome, Toru Hara, Tsuyoshi Yaita
Microparticles of radioactive cesium (Cs)-bearing silicate glass emitted from the Fukushima Daiichi nuclear power plant were investigated mainly using state-of-the-art energy-dispersive X-ray spectroscopy in scanning transmission electron microscopes. Precise elemental maps of the particles were obtained using double silicon drift detectors with a large collection angle of X-rays, and qualitative elemental analysis was performed using high-resolution X-ray spectroscopy with a microcalorimetry detector. Beside the substantial elements (O, Si, Cl, K, Fe, Zn, Rb, Sn and Cs) as previously reported, Mn and Ba were also common, though their amounts were small...
October 2016: Microscopy
Masashi Yamaguchi, Toshihiro Aoyama, Norihiro Yamada, Hiroji Chibana
Hydrophilicity/hydrophobicity of the surfaces of plasma-polymerized naphthalene film (Super Support Film, Nisshin EM Co. Ltd., Tokyo), carbon and formvar support films, and copper and nickel grids were quantitatively estimated by contact angles measured from diameters and heights of water droplets placed on various specimens. With treatment of glow discharge, the surfaces of plasma-polymerized naphthalene and carbon support films became fully hydrophilic in 20 s. They remained hydrophilic for 6 h. The surfaces of copper and nickel grids became fully hydrophilic with 60 s of glow discharge treatment...
October 2016: Microscopy
Tomas Tamulevičius, Linas Šimatonis, Orestas Ulčinas, Sigitas Tamulevičius, Egidijus Žukauskas, Regina Rekuvienė, Liudas Mažeika
Scanning acoustic microscopy (SAM) is used as a routine non-destructive test tool for different diagnostic examinations: detection of defects such as microcracks, delamination, disbonding, inclusions, subsurface features in materials such as pores and cracks. SAM can be operated in a wide frequency range from Megahertz to Gigahertz. SAM measurement spatial resolution is diffraction limited by the wavelength of the acoustic wave in particular medium and also depends on individual transducers geometry. Actual SAM spatial resolution can be determined by measuring calibrated lithographically formed microstructures in high acoustic impedance materials...
October 2016: Microscopy
Yuchuan Li, Nina P Hessvik, Niels C Danbolt, Torgeir Holen
Little is known about olfactory glands' regulation despite their presumed importance for normal functioning of the cilia of olfactory neurons. The aim of this study was to establish an assay for olfactory gland activation by using large-scale quantitative electron microscopy (EM). In addition we wanted to test the hypothesis that cholinergic drugs activate the olfactory glands, by using our newly established EM assay. In total, over 70 000 secretory gland vesicles were quantified in over 3000 cells. Olfactory gland cell size (40...
October 2016: Microscopy
(no author information available yet)
No abstract text is available yet for this article.
August 2016: Microscopy
(no author information available yet)
No abstract text is available yet for this article.
August 2016: Microscopy
(no author information available yet)
No abstract text is available yet for this article.
August 2016: Microscopy
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