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Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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https://www.readbyqxmd.com/read/30362932/establishment-of-monoclonal-antibody-pmab-202-against-horse-podoplanin
#1
Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Masato Sano, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Takuya Mizuno, Ken Maeda, Masato Fukui, Hiroyuki Harada, Mika K Kaneko, Yukinari Kato
Podoplanin (PDPN), a type I transmembrane glycoprotein, is expressed in several body tissues, including podocytes of renal glomerulus, type I alveolar cells of lung, and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) presented on platelets. Monoclonal antibodies (mAbs) against human-, mouse-, rat-, rabbit-, dog-, bovine-, and cat-PDPN have already been established. However, anti-horse PDPN mAbs have not yet been developed. In this study, we immunized mice with synthetic horse PDPN peptides and developed anti-horse PDPN mAbs...
November 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30362931/dgmab-6-antihuman-dgk%C3%AE-monoclonal-antibody-for-immunocytochemistry
#2
Tomoyuki Nakano, Satoshi Ogasawara, Toshiaki Tanaka, Yasukazu Hozumi, Atsumi Yamaki, Fumio Sakane, Yasuhito Shirai, Takuro Nakamura, Miyuki Yanaka, Shinji Yamada, Mika K Kaneko, Yukinari Kato, Kaoru Goto
Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). Since both DG and PA serve as lipidic second messengers, DGK plays a pivotal role in regulating the balance of two signaling pathways mediated by DG and PA in cellular functions. Reportedly, DGKγ, one of the 10 mammalian DGK isozymes, is involved in leukemic cell differentiation, mast cell function, and membrane traffic. Transfection studies using tagged expression vectors and immunohistochemistry on rat tissues revealed that DGKγ localizes to the cytoplasm, plasma membrane, and Golgi apparatus...
November 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30362930/the-augmenting-effects-of-the-tdna-insulator-on-stable-expression-of-monoclonal-antibody-in-chinese-hamster-ovary-cells
#3
Fatemeh Naderi, Mehrdad Hashemi, Hadi Bayat, Omid Mohammadian, Es'hagh Pourmaleki, Mohammad Hossein Etemadzadeh, Azam Rahimpour
Cell line development is one of the most critical steps in the production of complex recombinant therapeutic proteins such as monoclonal antibodies in mammalian cells. Generation of industrial cell lines is mainly based on the time-consuming and laborious process of selection and screening of a large number of clones. With the increasing demand for therapeutic proteins during the past years, more effort is invested to improve the efficiency of cell line development. In line with this premise, several studies employed expression vector engineering strategies based on incorporation of epigenetic regulatory elements, which can enhance the expression level and stability of the transgenes...
November 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30362929/generation-of-a-monoclonal-antibody-against-staphylococcal-superantigen-like-protein-5-ssl5-that-discriminates-ssl5-from-other-ssl-proteins
#4
Teruaki Oku, Hikari Soma, Chisato Kurisaka, Tsutomu Tsuji
Staphylococcus aureus secretes a family of exoproteins structurally homologous to bacterial superantigens, such as toxic shock syndrome toxin-1 (TSST-1), and those exoproteins are thus called staphylococcal superantigen-like proteins (SSLs). Recent studies have revealed that SSLs play roles in evasion of the host defense by disturbing host immune responses. We previously reported that staphylococcal superantigen-like protein 5 (SSL5; a member of the SSL family) inhibited matrix metalloproteinase-9 (MMP-9), which is crucial for leukocyte recruitment to sites of infection...
November 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30362928/detection-of-tiger-podoplanin-using-the-anti-cat-podoplanin-monoclonal-antibody-pmab-52
#5
Shinji Yamada, Shunsuke Itai, Yoshikazu Furusawa, Masato Sano, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Kayo Hisamatsu, Yoshimi Nakamura, Masato Fukui, Hiroyuki Harada, Takuya Mizuno, Yusuke Sakai, Satoshi Ogasawara, Takeshi Murata, Hiroaki Uchida, Hideaki Tahara, Mika K Kaneko, Yukinari Kato
Podoplanin (PDPN) is expressed in type I alveolar cells of lung but not in type II alveolar cells. PDPN is also known as a specific lymphatic endothelial cell marker because PDPN is not expressed in vascular endothelial cells. PDPNs of several animals have been characterized using specific anti-PDPN monoclonal antibodies (mAbs): PMab-1, PMab-2, PMab-32, PMab-38, PMab-44, and PMab-52 for mouse, rat, rabbit, dog, bovine, and cat PDPNs, respectively. In this study, we investigated the possible crossreaction between these anti-PDPN mAbs and tiger PDPN...
November 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30362927/novel-monoclonal-antibodies-against-native-human-collectin-l1-using-the-heteromeric-complex-cl-lk-as-immunogen
#6
Josephine B Aagaard, Karen B Bjerrum, Jonas H Graversen, Maiken L Henriksen, Soren W K Hansen
Collectin LK (CL-LK) is a recently described collectin complex, which upon binding to microbial glycoconjugates, activates the lectin pathway of the complement system, and thereby contributes to the removal of invading microorganisms. The complex consists of the two collectins; Collectin K1 (kidney 1 alias CL-11) and Collectin L1 (liver 1 alias CL-10). At present, most efforts have been made on the characterization of CL-K1, and little is known about the function of CL-L1 and its association with diseases. Deficiency of either of the two collectins is associated with the developmental syndrome 3MC, whereas increased plasma levels of CL-K1 are associated with disseminated intravascular coagulation...
November 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30362926/establishment-of-p38bf-a-core-fucose-deficient-mouse-canine-chimeric-antibody-against-dog-podoplanin
#7
Yukinari Kato, Takuya Mizuno, Shinji Yamada, Takuro Nakamura, Shunsuke Itai, Miyuki Yanaka, Masato Sano, Mika K Kaneko
Podoplanin (PDPN), a type I transmembrane sialoglycoprotein, is expressed in normal tissues, including lymphatic endothelial cells, pulmonary type I alveolar cells, and renal podocytes. The overexpression of PDPN in cancers is associated with hematogenous metastasis by interactions with the C-type lectin-like receptor 2 (CLEC-2). We have previously reported the development of a mouse monoclonal antibody (mAb) clone, PMab-38 (IgG1 , kappa), against dog PDPN (dPDPN). PMab-38 reacted strongly with canine squamous cell carcinomas and melanomas, but not with lymphatic endothelial cells, indicating its cancer specificity...
November 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30281392/advancing-freund-s-and-addavax-adjuvant-regimens-using-cpg-oligodeoxynucleotides
#8
David Hawksworth
Adjuvant can play an important role in vaccine formulation by aiding in the development of a robust immune response. In our hybridoma development work, we typically use both Freund's and AddaVax™ adjuvant regimens for mouse immunizations. While we have repeatedly shown success with our protocols, we continually seek to improve upon the titer and affinity of the serum antibody response. To that end, we evaluated the use of CpG oligodeoxynucleotides (CpG-DNA), a B cell stimulant, in our adjuvant regimens. Mice were immunized using our standard Freund's protocol (Adjulite Complete Freund's Adjuvant for the primary immunization followed by Adjulite Incomplete Freund's Adjuvant (AIFA) for all additional immunizations) or a test protocol using AIFA supplemented with CpG-DNA for all immunizations...
November 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30161019/monoclonal-antibody-specific-for-ovarian-tumor-domain-containing-protein-1
#9
(no author information available yet)
No abstract text is available yet for this article.
August 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30133349/evidence-for-multiple-applications-of-monoclonal-antibody-5g10
#10
Lilan Xie, Wangsheng Chen, Jianjun Li, Yi Li, Yaoming Li
We had earlier obtained a murine monoclonal antibody (mAb), termed 5G10, that bound to Salmonella flagellin (SF) and subsequently impaired the latter property of Toll-like receptor 5 (TLR5) signaling activation. Besides interrupting SF-mediated TLR5 activation, mAb 5G10 probably had other potential applications. In this study, we explored multiple functions of 5G10. A short peptide QRVRELAV (designated T5) derived from SF in either terminal of proteins was specifically recognized by 5G10. T5 tag expressed in eukaryotic cell was also detected by 5G10 when analyzed by Western blot, immunofluorescence assay (IFA), and fluorescent-activated cell sorting (FACS)...
August 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30132720/development-and-characterization-of-monoclonal-antibodies-against-nitro-166-tyrosine-of-high-density-lipoprotein-apolipoprotein-a1
#11
Guntur Divya, N S Jayaprakash, Krishnan Venkataraman
Apolipoprotein A1 (ApoA1) of the high-density lipoprotein (HDL) plays a cardinal role in alleviating atherosclerosis in various ways. Its role in reverse cholesterol transport is preeminent. However, the ApoA1 undergoes oxidation under chronic inflammatory conditions and these oxidations are mediated by myeloperoxidase. It has been reported that the oxidation of the amino acids such as methionine, tyrosine, and tryptophan residues at specific sites of ApoA1 renders it not only dysfunctional but also proinflammatory and proatherogenic...
August 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30130141/generation-of-rat-monoclonal-antibodies-against-a-deubiquitinase-ovarian-tumor-domain-containing-protein-1
#12
Daisuke Oikawa, Masayuki Shiota, Eiji Goto, Keidai Komakura, Hideki Wanibuchi, Fuminori Tokunaga
Ovarian tumor domain-containing protein 1 (OTUD1), an OTU-family deubiquitinating enzyme, has been reported to be involved in cancer progression through the regulation of p53 and SMAD7. However, the precise pathophysiological functions of OTUD1 remain elusive. Here, we report the establishment of OTUD1-specific monoclonal antibodies (mAbs), using the rat medial iliac lymph node method. The generated antibodies recognize the N-terminal portion (aa. 1-290) of human and mouse OTUD1 proteins. In addition, immunofluorescent staining and subcellular fractionation analyses using these antibodies indicated that OTUD1 is predominantly localized in the cytosol...
August 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30088964/elucidation-of-critical-epitope-of-anti-rat-podoplanin-monoclonal-antibody-pmab-2
#13
Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Masato Fukui, Hiroyuki Harada, Mika K Kaneko, Yukinari Kato
Rat podoplanin (rPDPN) is a recognized lymphatic endothelial cell marker and is expressed on the podocytes of kidney and type I lung alveolar cells. rPDPN is a type I transmembrane sialoglycoprotein and induces platelet aggregation via the C-type lectin-like receptor-2 of platelets. It comprises four platelet aggregation-stimulating (PLAG) domains: PLAG1-3, present in the N-terminus, and PLAG4, in the center of the PDPN protein. Previously, we developed a mouse anti-rPDPN monoclonal antibody clone, PMab-2, by immunizing the PLAG2 and PLAG3 domains of rPDPN...
August 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/30004263/epitope-mapping-of-anti-telomerase-reverse-transcriptase-monoclonal-antibodies
#14
Yoshikazu Furusawa, Shunsuke Itai, Shinji Yamada, Mika K Kaneko, Yukinari Kato
Telomerase reverse transcriptase (TERT) is the main catalytic subunit of telomerase-associated protein machinery. Upregulation of TERT at the transcriptional level results in immortal cell phenotype associated with cancer. To date we have developed eight anti-TERT monoclonal antibodies (mAbs) (TMab-4, TMab-5, TMab-6, TMab-7, TMab-9, TMab-10, TMab-11, and TMab-12) by immunizing mice with synthetic peptides (302 -QHHAGPPSTSRPPRPWDTPC-321 ) of TERT. We further characterized those epitopes using enzyme-linked immunosorbent assay, and here we discuss the critical epitope of an anti-TERT mAb, which is applicable for immunohistochemical analysis...
August 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29939836/thinking-cancer
#15
Anastas Pashov, Cinthia Violeta Hernandez Puente, Saddam Mohammed Ibrahim, Behjatolah Monzavi-Karbassi, Issam Makhoul, Thomas Kieber-Emmons
Evolutionary theories are necessarily invoked for understanding cancer development at the level of species, at the level of cells and tissues, and for developing effective therapies. It is crucial to view cancer in a Darwinian light, where the differential survival of individual cells is based on heritable variations. In the process of this somatic evolution, multicellularity controls are overridden by cancer cells, which become increasingly autonomous. Ecological epigenetics also helps understand how rogue cells that have basically the same DNA as their normal cell counterpart overcome the tissue homeostasis...
June 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29939835/monoclonal-antibody-mab-sz-189-against-recombinant-human-clec-2-protein
#16
(no author information available yet)
No abstract text is available yet for this article.
June 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29901420/a-bioassay-for-optimization-of-macrophage-conditioned-medium-as-a-culture-supplement-to-promote-hybridoma-cell-survival-and-growth
#17
Robert Hnasko, Alice V Lin, Larry Stanker, Jeffery McGarvey
Macrophage-conditioned medium (MCM) is an important cell culture supplement used to support the survival and growth of newly fused hybridoma cells. The use of macrophage cells, as a part of hybridoma technology, has proven to be an effective and inexpensive source of growth factors that promote the early survival and growth of hybridoma cells. Despite the widespread use of MCM as a hybridoma culture supplement, there is limited guidance and standardization for MCM production to achieve optimal hybridoma support...
June 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29901419/association-with-monoclonal-antibody-promotes-intracellular-delivery-of-lycopene
#18
Ivan M Petyaev, Naylia A Zigangirova, Valeriy V Tsibezov, Elena Y Morgunova, Natalia E Bondareva, Nigel H Kyle, Yuriy K Bashmakov
Incubation of B10.MLM cells, a cell line of alveolar macrophages, with lycopene, a carotenoid, leads to an increase of lycopene content in their microsomal fraction. That increase was higher and developed faster when the cells were incubated with immune complexes formed by lycopene and mAb 6B9 (L-6B9 mAb), a monoclonal hapten-specific antibody raised against lycopene, as compared with dimethyl sulfoxide (DMSO)-dissolved lycopene (DMSO-L). Moreover, incubation of B10.MLM cells with L-6B9 mAb complexes was accompanied by more efficient accumulation of lipid droplets in the cultured cells and more significant inhibition of mRNA for 3-hydroxy-3-methylglutaryl-coenzyme (HMG-CoA) reductase, a rate-limiting enzyme of cholesterol biosynthesis known to be targeted by lycopene...
June 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29901405/non-invasive-immunofluorescence-assessment-of-lycopene-supplementation-status-in-skin-smears
#19
Ivan M Petyaev, Naylia A Zigangirova, Dmitry Pristensky, Marina Chernyshova, Valeriy V Tsibezov, Natalya E Chalyk, Elena Y Morgunova, Nigel H Kyle, Yuriy K Bashmakov
Circulating lycopene level is negatively associated with the prevalence of cardiovascular disease, cancers (prostate and breast), type 2 diabetes mellitus, and aging. Traditionally, lycopene is measured in biological specimens by a combination of high-performance liquid chromatography (HPLC) and mass spectrometry methods. Moreover, as we recently reported, tissue/cell lycopene depositions can be observed by the immunohistochemistry method with a newly developed monoclonal antibody (mAb) against lycopene. A main objective of this study is to evaluate the performance of a new noninvasive immunofluorescence (IF) lycopene quantification skin test with mAbs against lycopene versus HPLC lycopene assay of serum lycopene in volunteers subjected to lycopene supplementation which represents a novel approach to lycopene measurement methodology...
June 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/29813003/preparation-and-application-of-a-monoclonal-antibody-against-chicken-trim62
#20
Xiaoman Wang, Yongzhen Zhao, Ling Li, Ziqiang Cheng, Guihua Wang
TRIM62 is a member of the tripartite interaction motif (TRIM) family and exerts crucial roles in innate immune response and cancer. To investigate the relationship between its distribution and avian retrovirus replication, in the present study, a monoclonal antibody (mAb) against chicken TRIM62 was generated. The open reading frame of chicken TRIM62 was amplified by polymerase chain reaction (PCR) and inserted into the expression vector pET-28a. The recombinant expression vectors were transformed into Escherichia coli BL21 (DE3)...
June 2018: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
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