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Cell Journal

Massoud Seifi, Ali Lotfi, Mohammad Reza Badiee, Zahra Abdolazimi, Parisa Amdjadi, Majid Bargrizan
OBJECTIVE: Orthodontically induced inflammatory root resorption (OIIRR) is an undesirable sequel of tooth movement after sterile necrosis that takes place in periodontal ligament due to blockage of blood vessels following exertion of orthodontic force. This study sought to assess the effect of an angiogenic cytokine on OIIRR in rat model. MATERIALS AND METHODS: In this experimental animal study, 50 rats were randomly divided into 5 groups of 10 each: E10, E100 and E1000 receiving an injection of 10, 100 and 1000 ng of basic fibroblast growth factor (bFGF), respectively, positive control group (CP) receiving an orthodontic appliance and injection of phosphate buffered saline (PBS) and the negative control group (CN) receiving only the anesthetic agent...
July 2016: Cell Journal
Zahra Nouri, Fatemeh Karami, Nadia Neyazi, Mohammad Hossein Modarressi, Roya Karimi, Mohammad Reza Khorramizadeh, Behrooz Taheri, Elahe Motevaseli
OBJECTIVE: Lactobacilli are a group of probiotics with beneficial effects on prevention of cancer. However, there is scant data in relation with the impacts of probiotics in late-stage cancer progration, especially metastasis. The present original work was aimed to evaluate the anti-metastatic and anti-proliferative activity of lactobacillus rhamnosus supernatant (LRS) and lactobacillus crispatus supernatant (LCS) on the human cervical and colon adenocarcinoma cell lines (HeLa and HT-29, respectively)...
July 2016: Cell Journal
Natasa Ristic, Darko Stevanovic, Dejan Nesic, Vladimir Ajdzanovic, Rastko Rakocevic, Ivana Jaric, Verica Milosevic
[This corrects the article on p. 711 in vol. 17, PMID: 26862530.].
July 2016: Cell Journal
Masoumeh Mousavinejad, Peter W Andrews, Elham Kargar Shoraki
Stem cells can be valuable model systems for drug discovery and modelling human diseases as well as to investigate cellular interactions and molecular events in the early stages of development. Controlling the differentiation of stem cells into specific germ layers provides a potential source of highly specialized cells for therapeutic applications. In recent years, finding individual properties of stem cells such as their ultimate self-renewal capacity and the generation of particular cell lines by differentiation under specific culture conditions underpins the development of regenerative therapies...
July 2016: Cell Journal
AliReza Alizadeh, Zeinab Taleb, Bita Ebrahimi, Vahid Esmaeili, Abdolhossein Shaverdi, Javad Nasr, Abolfazl Kheimeh, Reza Salman Yazdi
OBJECTIVE: Although key roles for dietary vitamin E (VITE) and fatty acid (FA) in fertility have been confirmed, limited data are available on the effects of VITE alone, or a constant level of VITE supplemented by dietary omega-6 and omega-3 FAs in combination on male reproduction. Consequently in this paper, the effects of VITE, sunflower oil, fish oil and their combination on rat sperm were investigated. MATERIALS AND METHODS: We divided 50 mature male Wistar rats into 5 groups (n=10) in a experimental completely randomized design for eight weeks: i...
July 2016: Cell Journal
Maryam Soleimani Farsani, Mandana Behbahani, Hamid Zarkesh Isfahani
OBJECTIVE: The genus Thymus L. is a cushion plant that was previously used for the treatment of bronchitis and rheumatism. The present investigation was carried out to study the effects of root, shoot, leaf and seed extracts of five Thymus species and subspecies on peripheral blood mononuclear cells (PBMCs) toxicity and HIV-1 replication. MATERIALS AND METHODS: In this experimental study, the activity of the Thymus extracts on HIV-1 replication and lymphocytes population were examined respectively using HIV-1 p24 Antigen kit and flow-cytometer...
July 2016: Cell Journal
Sedigheh Ganji-Harsini, Mozafar Khazaei, Zahra Rashidi, Ali Ghanbari
OBJECTIVE: Thymoquinone (TQ), as the main component of Nigella Sativa plant, shows anticancer properties. This study was aimed to evaluate the combined effect of TQ and Tamoxifen (TAM) on viability and apoptosis of human breast cancer cell lines. MATERIALS AND METHODS: In this experimental study, estrogen positive MCF-7 and estrogen negative MDA-MB-231 human breast cancer cell lines were induced by TAM (2 µM) or different doses of TQ (50, 75, 100, 150 µM), individually or in combination...
July 2016: Cell Journal
Ali Esfandiary, Zahra Taherian-Esfahani, Atieh Abedin-Do, Reza Mirfakhraie, Mahdieh Shirzad, Soudeh Ghafouri-Fard, Elahe Motevaseli
OBJECTIVE: Hypoxia-Inducible Factor (HIF)-1 plays an essential role in the body's response to low oxygen concentrations and regulates expression of several genes implicated in homeostasis, vascularization, anaerobic metabolism as well as immunological responses. Increased levels of HIF-1α are associated with increased proliferation and more aggressive breast tumor development. Lactobacilli have been shown to exert anti-cancer effects on several malignancies including breast cancer. However, the exact mechanism of such effect is not clear yet...
July 2016: Cell Journal
Saeed Talebi, Mona Entezam, Neda Mohajer, Golnaz-Ensieh Kazemi-Sefat, Masoumeh Razipour, Somayeh Ahmadloo, Aria Setoodeh, Mohammad Keramatipour
OBJECTIVE: The phenylalanine hydroxylase (PAH) locus has high linkage disequilibrium. Haplotypes related to this locus may thus be considered sufficiently informative for genetic diagnosis and carrier screening using multi-allelic markers. In this study, we present an efficient method for haplotype analysis of PAH locus using multiplexing dyes. In addition, we explain how to resolve the dye shift challenge in multiplex short tandem repeat (STR) genotyping. MATERIALS AND METHODS: One hundred family trios were included in this descriptive study...
July 2016: Cell Journal
Maryam Peymani, Kamran Ghaedi, Shiva Irani, Mohammad Hossein Nasr-Esfahani
OBJECTIVE: Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the PPAR nuclear receptor superfamily. Although PPARγ acts as a master transcription factor in adipocyte differentiation, it is also associated with a variety of cell functions including carbohydrate and lipid metabolism, glucose homeostasis, cell proliferation and cell differentiation. This study aimed to assess the expression level of PPARγ in order to address its role in cardiac cell differentiation of mouse embryonic stem cells (mESCs)...
July 2016: Cell Journal
Khadijeh Falahzadeh, Maryam Shahhoseini, Parvaneh Afsharian
OBJECTIVE: Nuclear actin is involved in transcription regulation by recruitment of histone modifiers and chromatin remodelers to the regulatory regions of active genes. In recent years, further attention has been focused on the role of actin as a nuclear protein in transcriptional processes. In the current study, the epigenetic role of nuclear actin on transcription regulation of two stemness (OCT4 and NANOG) and two differentiation) NESTIN and PAX6) marker genes was evaluated in a human embryonal carcinoma cell line (NT2) before and after differentiation induction...
July 2016: Cell Journal
Behnaz Sadeghzadeh Oskouei, Maryam Pashaiasl, Mohammad Hasan Heidari, Mohammad Salehi, Hadi Veladi, Firuz Ghaderi Pakdel, Parviz Shahabi, Marefat Ghaffari Novin
OBJECTIVE: In conventional assisted reproductive technology (ART), oocytes are cultured in static microdrops within Petri dishes that contain vast amounts of media. However, the in vivo environment is dynamic. This study assesses in vitro oocyte maturation through the use of a new microfluidic device. We evaluate oocyte fertilization to the blastocyct stage and their glutathione (GSH) contents in each experimental group. MATERIALS AND METHODS: In this experimental study, we established a dynamic culture condition...
July 2016: Cell Journal
Abbas Kiani-Esfahani, Sedigheh Kazemi Sheykhshabani, Maryam Peymani, Motahare-Sadat Hashemi, Kamran Ghaedi, Mohammad Hossein Nasr-Esfahani
OBJECTIVE: Annexin A1 (ANXA1) is suggested to have anti-inflammatory function. However, the precise function of ANXA1 has remained unclear. In this study, we therefore examined the potency of ANXA1 in regulating reactive oxygen species (ROS) production and suppressing pro-inflammatory responses in PC12 cells induced by 1-methyl-4-phenylpyridinium (MPP+). MATERIALS AND METHODS: In this experimental study, cDNA of ANXA1 was cloned and inserted to the PGL268 pEpi-FGM18F vector to produce a recombinant PGL/ANXA1 vector for transfection into the PC12 cells...
July 2016: Cell Journal
Yousef Mortazavi, Fatemeh Sheikhsaran, Gholamreza Khamisipour Khamisipour, Masoud Soleimani, Ali Teimuri, Somayeh Shokri
OBJECTIVE: Treatment and repair of neurodegenerative diseases such as brain tumors, spinal cord injuries, and functional disorders, including Alzheimer's disease, are challenging problems. A common treatment approach for such disorders involves the use of mesenchymal stem cells (MSCs) as an alternative cell source to replace injured cells. However, use of these cells in hosts may potentially cause adverse outcomes such as tumorigenesis and uncontrolled differentiation. In attempt to generate mesenchymal derived neural cells, we have infected MSCs with recombinant lentiviruses that expressed nerve growth factor (NGF) and assessed their neural lineage genes...
July 2016: Cell Journal
Abdolamir Allameh, Maryam Jazayeri, Maryam Adelipour
OBJECTIVE: In vivo and in vitro stem cell differentiation into endothelial cells is a promising area of research for tissue engineering and cell therapy. MATERIALS AND METHODS: We induced human mesenchymal stem cells (MSCs) to differentiate to endothelial cells that had the ability to form capillaries on an extracellular matrix (ECM) gel. Thereafter, the differentiated endothelial cells at early stage were characterized by expression of specific markers such as von Willebrand factor (vWF), vascular endothelial growth factor (VEGF) receptor 2, and CD31...
July 2016: Cell Journal
Narges Obeidi, Ali Akbar Pourfathollah, Masoud Soleimani, Mahin Nikougoftar Zarif, Fatemeh Kouhkan
OBJECTIVE: MicroRNAs (miRNAs) are small endogenous non-coding regulatory RNAs that control mRNAs post-transcriptionally. Several mouse stem cells miRNAs are cloned differentially regulated in different hematopoietic lineages, suggesting their possible role in hematopoietic lineage differentiation. Recent studies have shown that specific miRNAs such as Mir-451 have key roles in erythropoiesis. MATERIALS AND METHODS: In this experimental study, murine embryonic stem cells (mESCs) were infected with lentiviruses containing pCDH-Mir-451...
July 2016: Cell Journal
Mohammad Rafiee, Mohammad Reza Keramati, Hosein Ayatollahi, Mohammad Hadi Sadeghian, Mohieddin Barzegar, Ali Asgharzadeh, Mohsen Alinejad
OBJECTIVE: Signaling pathways such as extracellular regulated kinase/mitogen activated protein kinase (ERK/MAPK) have increased activity in leukemia. Ribosomal 6 kinase (RSK4) is a factor downstream of the MAPK/ERK pathway and an important tumor suppressor which inhibits ERK trafficking. Decrease in RSK4 expression has been reported in some malignancies, which leads to an increase in growth and proliferation and eventually poor prognosis. In this study we measured RSK4 expression rate in acute myeloid leukemia (AML)...
July 2016: Cell Journal
Shubing Zhang, Dewen Qiu, Jingjiang Liu, Zhijian Li
OBJECTIVE: Gastric cancer is a major health issue worldwide. Using a therapeutic approach, with minor side-effects, is very essential for the treatment of the gastric cancer. Shiraia bambusicola is a parasitic fungus which is widely used in China for curing several diseases with little side-effects. However, the mechanisms are not well understood yet. The aim of this study was to further understand the pharmacological mechanisms of Shiraia bambusicola and investigate whether it can be used for curing gastric cancer...
July 2016: Cell Journal
Mohammad Mahdi Ochi, Ghasem Amoabediny, Seyed Mahdi Rezayat, Azim Akbarzadeh, Bahman Ebrahimi
OBJECTIVE: This study aimed to evaluate a co-encapsulated pegylated nano-liposome system based on two herbal anti-tumor drugs, silibinin and glycyrrhizic acid, for delivery to a hepatocellular carcinoma (HCC) cell line (HepG2). MATERIALS AND METHODS: In this experimental study, co-encapsulated nano-liposomes by the thin layer film hydration method with HEPES buffer and sonication at 60% amplitude. Liposomes that co-encapsulated silibinin and glycyrrhizic acid were prepared with a specified molar ratio of dipalmitoylphosphatidylcholine (DPPC), cholesterol (CHOL), and methoxy-polyethylene glycol 2000 (PEG2000)-derived distearoyl phosphatidylethanolamine (mPEG2000-DSPE)...
July 2016: Cell Journal
Saeid Saeidimehr, Ammar Ebrahimi, Najmaldin Saki, Parisa Goodarzi, Fakher Rahim
Ageing is a complex process and a broad spectrum of physical, psychological, and social changes over time. Accompanying diseases and disabilities, which can interfere with cancer treatment and recovery, occur in old ages. MicroRNAs (miRNAs) are a set of small non-coding RNAs, which have considerable roles in post-transcriptional regulation at gene expression level. In this review, we attempted to summarize the current knowledge of miRNAs functions in ageing, with mainly focuses on malignancies and all underlying genetic, molecular and epigenetics mechanisms...
July 2016: Cell Journal
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