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Cell Journal

Mohammad Hassan Naseri, Hoda Madani, Seyed Hossein Ahmadi Tafti, Maryam Moshkani Farahani, Davood Kazemi Saleh, Hossein Hosseinnejad, Saeid Hosseini, Sepideh Hekmat, Zargham Hossein Ahmadi, Majid Dehghani, Alireza Saadat, Soura Mardpour, Seyedeh Esmat Hosseini, Maryam Esmaeilzadeh, Hakimeh Sadeghian, Gholamreza Bahoush, Ali Bassi, Ahmad Amin, Roghayeh Fazeli, Yaser Sharafi, Leila Arab, Mansour Movahhed, Saeid Davaran, Narges Ramezanzadeh, Azam Kouhkan, Ali Hezavehei, Mehrnaz Namiri, Fahimeh Kashfi, Ali Akhlaghi, Fattah Sotoodehnejadnematalahi, Ahmad Vosough Dizaji, Hamid Gourabi, Naeema Syedi, Abdol Hosein Shahverdi, Hossein Baharvand, Nasser Aghdami
This article published in Cell J (Yakhteh), Vol 20, No 2, Jul-Sep 2018, on pages 267-277, four affiliations (1, 4, 5, and 10) were changed based on authors request.
October 2018: Cell Journal
Mahdi Lotfipanah, Fereydoon Azadeh, Mehdi Totonchi, Reza Omani-Samani
Objective: Stem cells that have unlimited proliferation potential as well as differentiation potency are considered to be a promising future treatment method for incurable diseases. The aim of the present study is to evaluate the future trend of stem cell researches from researchers' viewpoints. Materials and Methods: This was a cross-sectional descriptive study on researchers involved in stem cell research at Royan Institute. We designed a questionnaire using a qualitative study based on expert opinion and a literature review...
October 2018: Cell Journal
Yushuang Yang, Jie Yang, Fenghua Sui, Pengfei Huo, Hailing Yang
Objective: This study used bioinformatics to determine genetic factors involved in progression of acute myocardial infarction (MI). Materials and Methods: In this prospective study, gene expression profile GSE59867 was downloaded from the Gene Expression Omnibus database, which contained 46 normal samples obtained from stable coronary artery disease patients (n=46) who were without history of MI (control) and 390 samples from patients (n=111) who had evolving ST-segment elevation myocardial infarction (STEMI) as the MI group...
October 2018: Cell Journal
Neda Ranjbar Kohan, Saeed Nazifi, Mohammad Reza Tabandeh, Maryam Ansari Lari
Objective: L-carnitine (LC) has been shown to protect cardiac metabolism in diabetes patients with cardiovascular diseases (CVDs). Apelin, a newly discovered adipocytokines, is an important regulator of cardiac muscle function; however, the role of the level of expression of Apelin axis in improvement of cardiac function by LC in diabetic patients, is not clear. In the present study, obese insulin-resistant rats were used to determine the effect of LC, when given orally with a high-calorie diet, on Apelin and Apelin receptor (Apj) expression in cardiac muscle...
October 2018: Cell Journal
Elham Farjad, Hamid Reza Momeni
Objective: Cadmium is an environmental pollutant which induces oxidative stress while silymarin as an antioxidant is able to scavenge free radicals. The aim of the present study was to investigate the effect of silymarin on oxidative stress markers and antioxidant defense system capacity in mice treated with cadmium chloride. Materials and Methods: In this experimental study, adult mice were divided into four groups as follow: i. Control, ii. Cadmium chloride (5 mg/kg b...
October 2018: Cell Journal
Mozafar Khazaei, Mona Pazhouhi, Saber Khazaei
Objective: Glioblastoma multiforme is the most malignant form of brain tumors. Trifolium pratense L. has been suggested for cancer treatment in traditional medicine. Here we have investigated the effects of T. pratense extract on glioblastoma multiforme cell line (U87MG). Materials and Methods: In this experimental study, the effect of T. pratense extract on cell viability was investigated using trypan blue staining, MTT assay, and lactate dehydrogenase activity measurement...
October 2018: Cell Journal
Neda Abedpour, Mojdeh Salehnia, Nassim Ghorbanmehr
Objective: The aim of the present study was to examine whether lysophosphatidic acid (LPA) could decrease cell death and improve in vitro culture (IVC) conditions in cultured vitrified mouse ovarian tissue. Materials and Methods: In this experimental study, we collected and randomly divided 7-day-old mouse ovarian tissues into vitrified and non-vitrified groups. The ovaries were cultured in the presence and absence of LPA for one week. Morphology and follicular development were evaluated by hematoxylin and eosin (H&E) and Masson's trichrome (MTC) staining...
October 2018: Cell Journal
Nassim Ghorbanmehr, Mojdeh Salehnia, Mahboobeh Amooshahi
Objective: The aim of present study is to determine the effects of supplementation of oocyte maturation medium with sodium selenite (SS) on oocyte mitochondrial DNA (mtDNA) copy number and reactive oxygen species (ROS) levels. Materials and Methods: In this experimental study, germinal vesicle (GV), metaphase I (MI), and metaphase II (MII) stage oocytes were recovered from 6-8 week old female mice after superovulation. Some of the GV oocytes were cultured and matured in the presence and absence of SS...
October 2018: Cell Journal
Sara Taleahmad, Seyedeh Nafiseh Hassani, Ghasem Hosseini Salekdeh, Hossein Baharvand
Objective: Pluripotent stem cells (PSCs), with the capacity to self-renew and differentiate into all other cell types, are of benefit in regenerative medicine applications. Tightly controlled gene expression networks and epigenetic factors regulate these properties. In this study, we aim to evaluate the metabolic signature of pluripotency under 2i and R2i culture conditions versus serum condition. Materials and Methods: In this experimental study, we investigated bioinformatics analysis of the shotgun proteomics data for cells grown under 2i, R2i, and serum culture conditions...
October 2018: Cell Journal
Zahra Azhdari Tafti, Mehdi Mahmoodi, Mohamad Reza Hajizadeh, Vahid Ezzatizadeh, Hossein Baharvand, Massoud Vosough, Abbas Piryaei
Objective: Recent advances in cell therapy have encouraged researchers to provide an alternative for treatment and restoration of damaged liver through using hepatocytes. However, these cells quickly lose their functional capabilities in vitro. Here, we aim to use the secretome of mesenchymal stromal cells (MSCs) to improve in vitro maintenance conditions for hepatocytes. Materials and Methods: In this experimental study, following serum deprivation, human adipose tissue-derived MSCs (hAT-MSCs) were cultured for 24 hours under normoxic (N) and hypoxic (H) conditions...
October 2018: Cell Journal
Marzieh Rahimipour, Mojdeh Salehnia, Mina Jafarabadi
Objective: This experimental study aimed to evaluate the effects of 17β-estradiol (E2) and progesterone (P4) on the interaction between mouse embryo and human endometrial mesenchymal stromal cells, and gene expressions related to implantation [αV and β3 integrins, interleukin-1 receptor (IL-1R), and leukemia inhibitory factor receptor (LIFR)] using an in vitro twodimensional model. Materials and Methods: In this experimental study, the endometrial stromal cells were isolated enzymatically and mechanically, and cultured to the fourth passage...
October 2018: Cell Journal
Azam Samadian, Mahdi Hesaraki, Sepideh Mollamohammadi, Behrouz Asgari, Mehdi Totonchi, Hossein Baharvand
Objective: Dual inhibition of mitogen-activated protein kinase (MAPK) kinase (also known as MEK) and transforming growth factor β (TGFβ) type I receptors by PD0325901 and SB431542, known as R2i has been introduced as a highly efficient approach to the generation of mouse embryonic stem cells (ESC). In the present study, we investigated the molecular mechanisms underlying ESC derivation in the R2i condition. Materials and Methods: In this experimental study, zona-free whole E3...
October 2018: Cell Journal
Zahra Ghasemi, Nima Naderi, Amir Shojaei, Nooshin Ahmadirad, Mohammad Reza Raoufy, Javad Mirnajafi-Zadeh
Objective: Electrical low frequency stimulation (LFS) is a new therapeutic method that moderates hyperexcitability during epileptic states. Seizure occurrence is accompanied by some changes in action potential (AP) features. In this study, we investigated the inhibitory action of LFS on epileptiform activity (EA) induced-changes in AP features in hippocampal CA1 pyramidal neurons. Materials and Methods: In this experimental study, we induced EA in hippocampal slices by increasing the extracellular potassium (K+) concentration to 12 mM...
October 2018: Cell Journal
Malihe Nakhaeifard, Maryam Haji Ghasem Kashani, Iran Goudarzi, Arezou Rezaei
Objective: Adipose derived stem cells (ASCs) secrete numerous neurotrophic factors and cytokines in conditioned medium (CM), which protect neurons by its antioxidative and trophic effects. This research assesses the neuroprotective effect of ASCCM on neurotrophins genes expressions and tyrosine hydroxylase positive (TH+ ) cell density in male Wistar rats lesioned by 6-hydroxydopamine (6-OHDA). Materials and Methods: In this experimental study, the groups consisted of lesioned and sham rats with unilateral injections of 20 μg of 6-OHDA neurotoxin and phosphate buffered saline (PBS) into the striatum, respectively...
October 2018: Cell Journal
Anna Minaidou, Paschalis Nicolaou, Kyproula Christodoulou
Objective: Deleterious variants in LRSAM1, a RING finger ubiquitin ligase which is also known as TSG101-associated ligase (TAL), have recently been associated with Charcot-Marie-Tooth disease type 2P (CMT2P). The mechanism by which mutant LRSAM1 contributes to the development of neuropathy is currently unclear. The aim of this study was to induce LRSAM1 deficiency in a neuronal cell model, observe its effect on cell growth and morphology and attempt to rescue the phenotype with ancestral and mutant LRSAM1 transfections...
October 2018: Cell Journal
Fatemeh Bahreini, Massoud Houshmand, Mohammad Hossein Modarressi, Seyed Mohammad Akrami
Objective: Pompe disease (PD) is a progressive neuromuscular disorder that is caused by glucosidase acid alpha (GAA) deleterious mutations. Mitochondrial involvement is an important contributor to neuromuscular diseases. In this study the sequence of MT-ATP 6/8 and Cytochrome C oxidase I/II genes along with the expression levels of the former genes were compared in classic and non-classic patients. Materials and Methods: In this case-control study, the sequence of MT-ATP 6/8 and Cytochrome C oxidase was analyzed by polymerase chain reaction (PCR)-Sanger sequencing and expression of MT-ATP genes were quantified by real time-PCR (RT-PCR) in 28 Pompe patients...
October 2018: Cell Journal
Jingming Zhao, Wei Cheng, Xigang He, Yanli Liu, Ji Li, Jiaxing Sun, Jinfeng Li, Fangfang Wang, Yufang Gao
Objective: The aim of this study was to identify the molecular subtypes of chronic obstructive pulmonary disease (COPD) and to prioritize COPD candidate genes using bioinformatics methods. Materials and Methods: In this bioinformatics study, the gene expression dataset GSE76705 (including 229 COPD samples) and known COPD-related genes (candidate genes) were downloaded from the Gene Expression Omnibus (GEO) and the Online Mendelian Inheritance in Man (OMIM) databases respectively...
October 2018: Cell Journal
Reza Ranjbaran, Mahin Nikogoftar Zarif, Sedigheh Sharifzadeh, Habibollah Golafshan, Ali Akbar Pourfathollah
Objective: Hemoglobin F (HbF) augmentation is considered a clinically beneficial phenomenon in β-hemoglobinopathies. Prevention of γ-globin gene silencing, inspired by the hereditary persistence of fetal hemoglobin, may be a suitable strategy to upregulate HbF expression in these patients. Therefore, our objective was to assess the potential feasibility of induced -117 G→A substitution in HBG promoter in prevention of transcriptional silencing of the γ-globin. Materials and Methods: In this experimental study, human peripheral blood-derived hematopoietic stem cells (HSCs) and the K562 cell line were differentiated to erythroid cells...
October 2018: Cell Journal
Gökhan Terzioğlu, Özlem Türksoy, Ömer Faruk Bayrak
Objective: Cancer stem cells (CSCs) have important roles in survival and chemoresistance. These cells are commonly recognized with CD44 and CD24 markers. In this study, we aimed to analyze the effects of mtDNA content on cell surface positivity for anti-CD24 and anti-CD44 antibodies and chemoresistance level in AGS, HGC-27 and MKN-45 gastric cancer (GC) cell lines and to determine a setpoint for mtDNA copy for each cell line. Materials and Methods: In this experimental study, we initially decreased mtDNA levels in AGS, HGC-27 and MKN-45 by EtBr treatment...
October 2018: Cell Journal
Palamadai Krishnan Suresh
Breast cancer (BC) is a widely prevalent form of neoplasia in women with fairly alarming mortality statistics. This aspect may be attributed, in part, to the current spatial and temporal heterogeneity-based limitations in therapies with possible recurrence of this tumour at primary and/or secondary sites. Such an extensive phenotypic heterogeneity in breast cancer is unlikely to be adequately or completely comprehended by an immuno-histopathology-based classification alone. This finding has warranted research and development in the area of microarray-based methods (i...
October 2018: Cell Journal
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