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Cell Journal

Oranous Bashti, Mehrdad Noruzinia, Masoud Garshasbi, Morteza Abtahi
This article published in Cell J (Yakhteh), Vol 20, No 1, Apr-Jun 2018, on pages 84-89, the labels of columns in "Figure 3B" was changed. The correct one is presented below.
July 2018: Cell Journal
Farah Talebi, Farideh Ghanbari Mardasi, Javad Mohammadi Asl, Ali Lashgari, Freidoon Farhadi
Norrie disease (ND) is a rare X-linked recessive disorder, which is characterized by congenital blindness and, in several cases, accompanied with mental retardation and deafness. ND is caused by mutations in NDP, located on the proximal short arm of the X chromosome (Xp11.3). The disease has been observed in many ethnic groups worldwide, however, no such case has been reported from Iran. In this study, we present the molecular analysis of two patients with ND and the subsequent prenatal diagnosis. Screening of NDP identified a hemizygous missense mutation (p...
July 2018: Cell Journal
Emad Nikkhah, Reza Safaralizadeh, Javad Mohammadiasl, Maryam Tahmasebi Birgani, Mohammad Ali Hosseinpour Feizi, Neda Golchin
Bardet-Biedl syndrome (BBS) is a pleiotropic and multisystemic disorder characterized by rod-cone dystrophy, polydactyly, learning difficulties, renal abnormalities, obesity and hypogonadism. This disorder is genetically heterogeneous. Until now, a total of nineteen genes have been identified for BBS whose mutations explain more than 80% of diagnosed cases. Recently, the development of next generation sequencing (NGS) technology has accelerated mutation screening of target genes, resulting in lower cost and less time consumption...
July 2018: Cell Journal
Mohammad Amin Behmanesh, Hosein Najafzadehvarzi, Seyedeh Mahsa Poormoosavi
OBJECTIVES: Bisphenol A (BPA), an endocrine-disrupting chemical, has been considered as a possible risk factor for fertility because it induces testicular toxicity. Thus, we sought to analyze the effect of Aloe vera as plant with antioxidant properties on tissues and oxidative stress parameters in male rats. MATERIALS AND METHODS: In this experimental study, 50 adult male Wistar rats (200 ± 20 g) have been used in this 56 day study. Animals were completely randomized and divided into five groups: A1 (control), A2 (vehicle control), A3 (Aloe vera gel 300 mg/kg), B1 (BPA 20 μg/kg bw) and B2 (Aloe vera gel+ BPA)...
July 2018: Cell Journal
Mohammad Hassan Naseri, Hoda Madani, Seyed Hossein Ahmadi Tafti, Maryam Moshkani Farahani, Davood Kazemi Saleh, Hossein Hosseinnejad, Saeid Hosseini, Sepideh Hekmat, Zargham Hossein Ahmadi, Majid Dehghani, Alireza Saadat, Soura Mardpour, Seyedeh Esmat Hosseini, Maryam Esmaeilzadeh, Hakimeh Sadeghian, Gholamreza Bahoush, Ali Bassi, Ahmad Amin, Roghayeh Fazeli, Yaser Sharafi, Leila Arab, Mansour Movahhed, Saeid Davaran, Narges Ramezanzadeh, Azam Kouhkan, Ali Hezavehei, Mehrnaz Namiri, Fahimeh Kashfi, Ali Akhlaghi, Fattah Sotoodehnejadnematalahi, Ahmad Vosough Dizaji, Hamid Gourabi, Naeema Syedi, Abdol Hosein Shahverdi, Hossein Baharvand, Nasser Aghdami
OBJECTIVES: The regenerative potential of bone marrow-derived mononuclear cells (MNCs) and CD133+ stem cells in the heart varies in terms of their pro-angiogenic effects. This phase II/III, multicenter and double-blind trial is designed to compare the functional effects of intramyocardial autologous transplantation of both cell types and placebo in patients with recent myocardial infarction (RMI) post-coronary artery bypass graft. MATERIALS AND METHODS: This was a phase II/III, randomized, double-blind, placebo-controlled trial COMPARE CPM-RMI (CD133, Placebo, MNCs - recent myocardial infarction) conducted in accordance with the Declaration of Helsinki that assessed the safety and efficacy of CD133 and MNCs compared to placebo in patients with RMI...
July 2018: Cell Journal
Abdolkhaleg Deezagi, Samira Shomali
OBJECTIVES: Tissue engineering today uses factors that can induce differentiation of mesenchymal stem cells (MSCs) into other cell types. However, the problem of angiogenesis in this differentiated tissue remains an unresolved area of research interest. The aim of this study was to investigate the effects of prostaglandin F-2α (PGF-2α) on the expression of vascular endothelial growth factor (VEGF) in human adipose tissue derived MSCs. MATERIALS AND METHODS: In this experimental research, human adipose tissue was digested using collagenase...
July 2018: Cell Journal
Mehdi Najar, Mohammad Fayyad-Kazan, Gordana Raicevic, Hussein Fayyad-Kazan, Nathalie Meuleman, Dominique Bron, Laurence Lagneaux
OBJECTIVES: We aimed at characterizing the transcription profiles of immunological receptors associated with the biology of mesenchymal stromal cells (MSCs). MATERIALS AND METHODS: In this experimental study, quantitative real time-polymerase chain reaction (qRTPCR) was performed to establish the transcription profiles of advanced glycation end-products (RAGE) receptor, C-type lectin receptors (CLRs, including DECTIN-1, DECTIN-2 and MINCLE), leukotriene B4 (LTB4) receptors (BLT1 and BLT2) and cysteinyl leukotrienes (CysLTs) receptors (CYSLTR1 and CYSLTR2) in distinct populations of MSCs grown under basic or inflammatory conditions...
July 2018: Cell Journal
Somayeh Keshavarzi, Mohammad Salehi, Fattaneh Farifteh-Nobijari, Taher Hosseini, Sara Hosseini, Alaleh Ghazifard, Marefat Ghaffari Novin, Vahid Fallah-Omrani, Mohsen Nourozian, Ahmad Hosseini
OBJECTIVES: We evaluated the effect of melatonin, as a potent antioxidant agent, on glutathione (GSH) and reactive oxygen species (ROS) levels, as well as histone H3 lysine 9 (H3K9), and H4 lysine 12 (H4K12) acetylation when added to oocytes culture medium. MATERIALS AND METHODS: In this experimental study, two in vitro and in vivo groups were used. In the in vitro group, cumulus oocyte complexes (COCs) from the ovaries of B6D2F1 mice were cultured in maturation medium containing two doses of melatonin (10-9 and 10-6 M) and without melatonin [control group treated with dimethyl sulfoxide (DMSO)] for 22-24 hour...
July 2018: Cell Journal
Rohiyeh Souldouzi, Mazdak Razi, Ali Shalizar Jalali, Ghader Jalilzadeh-Amin, Saeedeh Amani
OBJECTIVES: Pulegone (PGN) is a monoterpene ketone, whose metabolites exert several cytotoxic effects in various tissues. The present study was conducted in order to evaluate the (R)-(+) PGN-induced alterations in ovarian aromatization, proto-oncogenes and estrogen receptorα ( ERα) and ERβ receptors expressions. MATERIALS AND METHODS: In this experimental study, mature albino mice were divided into experimental (received 25 mg/kg, 50 mg/kg and 100 mg/kg PGN, orally for 35 days) and control (received 2% solution of Tween 80 as a PGN solvent, orally) groups...
July 2018: Cell Journal
Sara Eslamizadeh, Mansour Heidari, Shahram Agah, Ebrahim Faghihloo, Hossein Ghazi, Alireza Mirzaei, Abolfazl Akbari
OBJECTIVES: Colorectal cancer (CRC) is one of the most common cancers and a major cause of cancer-related death worldwide. The early diagnosis of colorectal tumors is one of the most important challenges in cancer management. MicroRNAs (miRNAs) have provided new insight into CRC development and have been suggested as reliable and stable biomarkers for diagnosis and prognosis. This study's objective was to analyze the differential expression of miRNAs at differentstages of CRC searching for possible correlation with clinicopathological features to examine their potential value as diagnostic biomarkers...
July 2018: Cell Journal
Mugdha Kunte, Krutika Desai
OBJECTIVES: Considering the bioactivities exhibited by microalgae, the effect of protein extract of Chlorella minutissimma (CP extract) was investigated on the expression of human matrix metalloproteinases-1 (MMP-1) in the breast cancer cell line MDA-MB231, and that of MMP-2 and -9 in hepatocellular cancer cell line HepG2 at different expression levels. The study aimed identification and analysis of inhibitory activity of microalgal components extracted from Chlorella minutissima against human MMPs...
July 2018: Cell Journal
Hossein Pashaiefar, Marjan Yaghmaie, Javad Tavakkoly-Bazzaz, Seyed Hamidollah Ghaffari, Kamran Alimoghaddam, Pantea Izadi, Ardeshir Ghavamzadeh
OBJECTIVES: Chromosomal translocations are among the most common mutational events in cancer development, especially in hematologic malignancies. However, the precise molecular mechanism of these events is still not clear. It has been recently shown that alternative non-homologous end-joining (alt-NHEJ), a newly described pathway for double-stranded DNA break repair, mediates the formation of chromosomal translocations. Here, we examined the expression levels of the main components of alt-NHEJ (PARP1 and LIG3) in acute myeloid leukemia (AML) patients and assessed their potential correlation with the formation of chromosomal translocations...
July 2018: Cell Journal
Mohammad Houshmand, Mozhde Nakhlestani Hagh, Masoud Soleimani, Amir Ali Hamidieh, Saeed Abroun, Mahin Nikougoftar Zarif
OBJECTIVES: In order to clarify the role of microRNAs (miRNA) in megakaryocyte differentiation, we ran a microRNA microarray experiment to measure the expression level of 961 human miRNA in megakaryocytes differentiated from human umbilical cord blood CD133+ cells. MATERIALS AND METHODS: In this experimental study, human CD133+ hematopoietic stem cells were collected from three human umbilical cord blood (UCB) samples, and then differentiated to the megakaryocytic lineage and characterized by flow cytometry, CFU-assay and ploidy analysis...
July 2018: Cell Journal
Milad Ahani-Nahayati, Saeed Solali, Karim Shams Asenjan, Ali Akbar Movassaghpour Akbari, Mehdi Talebi, Milad Zadi Heydarabad, Sina Baharaghdam, Majid Farshdousti Hagh
OBJECTIVES: DNA methylation is a well-studied epigenetic mechanism that is a potent arm of the gene expression controlling machinery. Since the hypoxic situation and the various cells of bone marrow microenvironment, e.g. mesenchymal stem cells, play a role in the in vivo and in vitro biology of leukemic cells, we decided to study the effects of hypoxia and mesenchymal stem cells (MSCs) on the promoter methylation pattern of BAX and BCL2 genes. MATERIALS AND METHODS: In this experimental study, the co-culture of MOLT-4 cells with MSCs and treatment with CoCl2 was done during 6, 12, and 24 hour periods...
July 2018: Cell Journal
Alireza Abdanipour, Behnaz Shahsavandi, Mohsen Alipour, Hadi Feizi
OBJECTIVES: Ghrelin is a peptide which has a proliferative and antiapoptotic effect in many cells including bone marrow stromal cells (BMSCs). Homeobox protein B4 (HOXB4) is a transcription factor involved in stem cell regeneration and survival. The aim of the study was to find out the efect of ghrelin on Hoxb4 expression in BMSCs. MATERIALS AND METHODS: In this experimental study, rat BMSCs were cultivated in Dulbecco's Modified Eagle Medium (DMEM). Passage three BMSCs were treated with ghrelin 100 μM for 48 hours...
July 2018: Cell Journal
Azadeh Seyedjoodaki, Fereshteh Alsahebfosoul, Nahid Eskandari, Vahid Shaygannejad, Mansour Salehi, Mohammad Kazemi, Mostafa Manian, Omid Mirmosayyeb, Mohammad Taghi Kardi
OBJECTIVES: Inflammation of the immune system and the central nervous system has been known as an important predisposing factor for Parkinson's disease (PD). Increased expression of OX40 protein (CD134) is a known factor for increased inflammation and initiation of NF-kappa-B signaling pathway in different diseases. We aimed to investigate the expression of OX40 at the transcript and serum protein levels. MATERIALS AND METHODS: Twenty individuals with PD and 20 healthy individuals, as controls, were enrolled in this casecontrol study...
July 2018: Cell Journal
Mehrafarin Fesharaki, Shahnaz Razavi, Laleh Ghasemi-Mobarakeh, Mohaddeseh Behjati, Reyhaneh Yarahmadian, Mohammad Kazemi, Hossein Hejazi
OBJECTIVES: This study aimed to isolate and culture SADS cells, investigate their neurogenic capacity and evaluate their application for nerve tissue engineering. MATERIALS AND METHODS: In this experimental study, SADS cells were isolated from human adipose tissue. After 7-day treatment of SADS cells with insulin, indomethacin and isobutylmethylxanthine, neurogenic differentiation of SADS cells was investigated. During this study, Poly (ε-caprolactone) (PCL) and PCL/gelatin nanofibrous scaffolds were fabricated using electrospinning and subsequently nanofibrous scaffolds were coated with platelet-rich plasma (PRP)...
July 2018: Cell Journal
Mohammad Reza Afarinesh, Gila Behzadi
OBJECTIVES: The aim of the present study is to investigate the effects of chronic whisker deprivation on possible alterations to the development of nitrergic neurons in the whisker part of the somatosensory (wS1) and motor (wM1) cortices in offspring with congenital hypothyroidism (CH). MATERIALS AND METHODS: In the experimental study, CH was induced by adding propylthiouracil to the rats drinking water from embryonic day 16 to postnatal day (PND) 60. In whisker-deprived (WD) pups, all the whiskers were trimmed from PND 1 to 60...
July 2018: Cell Journal
Maedeh Rouigari, Moein Dehbashi, Kamran Ghaedi, Meraj Pourhossein
OBJECTIVES: For the first time, we used molecular signaling pathway enrichment analysis to determine possible involvement of miR-126 and IRS-1 in neurotrophin pathway. MATERIALS AND METHODS: In this prospective study, Validated and predicted targets (targetome) of miR-126 were collected following searching miRtarbase ( and miRWalk 2.0 databases, respectively. Then, approximate expression of miR-126 targeting in Glioma tissue was examined using UniGene database (http://www...
July 2018: Cell Journal
Hamid Choobineh, Mahsa Kazemi, Mohammad Ali Sadighi Gilani, Tahereh Heydari, Saeed Shokri, Mahshid Bazrafkan, Gholamreza Hassanzadeh
OBJECTIVES: This study evaluated the effects of exogenous testosterone molecule-1 (CADM1) pathological defect during early and chronic periods of spinal cord injury (SCI). MATERIALS AND METHODS: In this experimental study, Testosterone was administered immediately or after one week of SCI induction. Along with quantification of CADM1 gene expression and its immunoreactivity, we evaluated sperm parameters and serum testosterone level post-SCI. RESULTS: Different grades of abnormalities in sperm parameters and testis architecture were observed along with significant reductions in the level of CADM1 expression and its immunoreactivity in the seminiferous tubules of both acute and chronic SCI groups...
July 2018: Cell Journal
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