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Cell Journal

Mohieddin Jafari, Naser Ansari-Pour
Currently, numerous papers are published reporting analysis of biological data at different omics levels by making statistical inferences. Of note, many studies, as those published in this Journal, report association of gene(s) at the genomic and transcriptomic levels by undertaking appropriate statistical tests. For instance, genotype, allele or haplotype frequencies at the genomic level or normalized expression levels at the transcriptomic level are compared between the case and control groups using the Chi-square/Fisher's exact test or independent (i...
January 2019: Cell Journal
Seyedeh Parisa Chavoshi Tarzjani, Seyed Abol Hassan Shahzadeh Fazeli, Mohammad Hossein Sanati, Seyed Massood Nabavi
Multiple sclerosis (MS) is a chronic disease of the central nervous system and one of the most common causes of neurological disability among those aged 20-40 years, particularly in women. Major histocompatibility complex (MHC) Class II genes are known to be involved in the development of MS. One of the important groups of this complex is the HSP gene family, especially HSP70, which is induced under stress conditions. The aim of the present case-control study was to determine the association between the heat shock protein 70 (HSP70) and risk of MS in Iranian patients by genotyping the rs1061581 gene polymorphism...
January 2019: Cell Journal
Seyed Massood Nabavi, Leila Arab, Neda Jarooghi, Tina Bolurieh, Fatemeh Abbasi, Soura Mardpour, Vajihe Azimyian, Fatemeh Moeininia, Saman Maroufizadeh, Leila Sanjari, Seyedeh Esmat Hosseini, Nasser Aghdami
Objective: Amyotrophic lateral sclerosis (ALS) is the most severe disorder within the spectrum of motor neuron diseases (MND) that has no effective treatment and a progressively fatal outcome. We have conducted two clinical trials to assess the safety and feasibility of intravenous (IV) and intrathecal (IT) injections of bone marrow derived mesenchymal stromal cells (BM-MSCs) in patients with ALS. Materials and Methods: This is an interventional/experimental study...
January 2019: Cell Journal
Fereshteh Rahimi, Somayeh Fardindoost, Naser Ansari-Pour, Fatemeh Sepehri, Farideh Makiyan, Azizollah Shafiekhani, Ali Hossein Rezayan
Objective: Substantial effort has been put into designing DNA-based biosensors, which are commonly used to detect presence of known sequences including the quantification of gene expression. Porous silicon (PSi), as a nanostructured base, has been commonly used in the fabrication of optimally transducing biosensors. Given that the function of any PSi-based biosensor is highly dependent on its nanomorphology, we systematically optimized a PSi biosensor based on reflectometric interference spectroscopy (RIS) detecting the high penetrance breast cancer susceptibility gene, BRCA1...
January 2019: Cell Journal
Hussain Habibi, Amir Atashi, Saeid Abroun, Mehrdad Noruzinia
Objective: Hemoglobinopathies such as beta-thalassemia and sickle cell disease (SCD) are inherited disorders that are caused by mutations in beta-globin chain. Gamma-globin gene reactivation can ameliorate clinical manifestations of betathalassemia and SCD. Drugs that induce fetal hemoglobin (HbF) can be promising tools for treatment of beta-thalassemia and SCD patients. Recently, it has been shown that Simvastatin (SIM) and Romidepsin (ROM) induce HbF. SIM is a BCL11a inhibitor and ROM is a HDAC inhibitor and both of these drugs are Food and Drug Administration (FDA)-approved for hypercholesterolemia and cutaneous T-cell lymphoma respectively...
January 2019: Cell Journal
Fatemeh Bahreini, Ali Reza Soltanian
Objective: We sought to apply Shannon's entropy to determine colorectal cancer genes in a microarray dataset. Materials and Methods: In the retrospective study, 36 samples were analysed, 18 colorectal carcinoma and 18 paired normal tissue samples. After identification of the gene fold-changes, we used the entropy theory to identify an effective gene set. These genes were subsequently categorised into homogenous clusters. Results: We assessed 36 tissue samples...
January 2019: Cell Journal
Sara Bitarafan, Mohsen Yari, Mohammad Ali Broumand, Sayyed Mohammad Hossein Ghaderian, Mahnoosh Rahimi, Reza Mirfakhraie, Faezeh Azizi, Mir Davood Omrani
Objective: Considerable research shows that long non-coding RNAs, those longer than 200 nucleotides, are involved in several human diseases such as various cancers and cardiovascular diseases. Their significant role in regulating the function of endothelial cells, smooth muscle cells, macrophages, vascular inflammation, and metabolism indicates the possible effects of lncRNAs on the progression of atherosclerosis which is the most common underlying pathological process responsible for coronary artery disease (CAD)...
January 2019: Cell Journal
Zeynep Ermiş Karaali, Gonca Candan, Mehmet Burak Aktuğlu, Mustafa Velet, Arzu Ergen
Objective: Innate immunity factors are associated with type 2 diabetes (T2DM) and its complications. Therefore, T2DM has been suggested to be an immune-dependent disease. Elevated fasting glucose level and higher concentrations of innate immunity soluble molecules are not only related with insulin resistance, but inflammation is also an important factor in beta cell dysfunction in T2DM. Toll-like receptor 2 (TLR-2), which has an important role in inducing innate immune cells, is thought to have suppressive roles on immune responses in T2DM...
January 2019: Cell Journal
Alaleh Ghazifard, Mohammad Salehi, Marefat Ghaffari Novin, Mojgan Bandehpour, Somayeh Keshavarzi, Vahid Fallah Omrani, Maryam Dehghani-Mohammadabadi, Reza Masteri Farahani, Ahmad Hosseini
Objective: Over the last years, vitrification has been widely used for oocyte cryopreservation, in animals and humans; however, it frequently causes minor and major epigenetic modifications. The effect of oocyte vitrification on levels of acetylation of histone H4 at lysine 12 (AcH4K12), and histone acetyltransferase (Hat) expression, was previously assessed; however, little is known about the inhibition of Hat expression during oocyte vitrification. This study evaluated the effect of anacardic acid (AA) as a Hat inhibitor on vitrified mouse oocytes...
January 2019: Cell Journal
Husamaldeen Alsalim, Farnoosh Jafarpour, Faezeh Ghazvini Zadegan, Mohammad Hossein Nasr-Esfahani, Amir Niasari-Naslaji
Objective: In the present study, we investigated the possible epigenotoxic effect of dimethyl sulfoxide (DMSO) on buffalo fibroblast cells and on reconstructed oocytes during buffalo-bovine interspecies somatic cell nuclear transfer (iSCNT) procedure and its effect on rate and quality of blastocyst which derived from these reconstructed oocytes. Materials and Methods: In this experimental study, cell viability of buffalo fibroblasts was assessed after exposure to various concentration (0...
January 2019: Cell Journal
Maryam Hezavehei, Homa Mohseni Kouchesfahani, Abdol Hossein Shahverdi, Mohsen Sharafi, G Hasem Hosseini Salekdeh, Poopak Eftekhari-Yazdi
Objective: A recent innovative approach, based on induction of sublethal oxidative stress to enhance sperm cryosurvival, has been applied before sperm cryopreservation. The purpose of this study was to investigate the effects of different induction times of sublethal oxidative stress before cryopreservation on human post-thawed sperm quality. Materials and Methods: In this experimental study, we selected semen samples (n=20) from normozoospermic men according to 2010 World Health Organization (WHO) guidelines...
January 2019: Cell Journal
Azam Dalman, Mehdi Totonchi, Mojtaba Rezazadeh Valojerdi
Objective: In this study, we have examined human theca stem cells (hTSCs) in vitro differentiation capacity into human oocyte like cells (hOLCs). Materials and Methods: In this interventional experiment study, hTSCs were isolated from the theca layer of small antral follicles (3-5 mm in size). Isolated hTSCs were expanded and cultured in differentiation medium, containing 5% human follicular fluid, for 50 days. Gene expressions of PRDM1, PRDM14, VASA, DAZL, OCT4, ZP1, 2, 3 GDF9, SCP3 and DMC1 were evaluated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) on days 0, 18, and 25 after monoculture as well as one week after co-culture with human granulosa cells (hGCs)...
January 2019: Cell Journal
Shahnaz Razavi, Farnaz Khadivi, Fatemeh Hashemi, Abbas Bakhtiari
Objective: The incidence rate of testicular cancer among young males is high. Co-administration of bleomycin, etoposide and cisplatin (BEP) has increased survival rate of patients with testicular cancer. Although BEP is one of the most effective treatment for testicular cancer, but it severely affects the reproductive system that ultimately leads to infertility. In addition to its antioxidant activity, zinc has an important role in progression of spermiogenesis. This study aimed to evaluate the effect of zinc on sperm parameters, chromatin condensation and testicular structure after BEP treatment...
January 2019: Cell Journal
Mahdi Mohaqiq, Mansoureh Movahedin, Zohreh Mazaheri, Naser Amirjannati
Objective: In vitro transplantation (IVT) of spermatogonial stem cells (SSCs) is one of the most recent methods in transplantation in recent decades. In this study, IVT and SSCs homing on seminiferous tubules of host testis in organ culture have been studied. Materials and Methods: In this experimental study, human SSCs were isolated and their identities were confirmed by tracking their promyelocytic leukemia zinc finger (PLZF) protein. These cells were transplanted to adult azoospermia mouse testes using two methods, namely, IVT and in vivo transplantation as transplantation groups, and testes without transplantation of cells were assigned in the control group...
January 2019: Cell Journal
Mehrdad Ghorbanlou, Alireza Abdanipour, Reza Shirazi, Nasim Malekmohammadi, Saeed Shokri, Reza Nejatbakhsh
Objective: Non-obstructive azoospermia is mostly irreversible. Efforts to cure this type of infertility have led to the application of stem cells in the reproduction field. In the present study, testicular cell-mediated differentiation of male germ-like cells from bone marrow-derived mesenchymal stem cells (BM-MSCs) in an in vitro indirect co-culture system is investigated. Materials and Methods: In this experimental study, mouse BM-MSCs were isolated and cultured up to passage three...
January 2019: Cell Journal
Sadaf Vahdat, Sara Pahlavan, Nasser Aghdami, Behnaz Bakhshandeh, Hossein Baharvand
Objective: Cardiovascular progenitor cells (CPCs) are introduced as one of the promising cell sources for preclinical studies and regenerative medicine. One of the earliest type of CPCs is cardiogenic mesoderm cells (CMCs), which have the capability to generate all types of cardiac lineage derivatives. In order to benefit from CMCs, development of an efficient culture strategy is required. We aim to explore an optimized culture condition that uses human embryonic stem cell (hESC)-derived CMCs...
January 2019: Cell Journal
Sudabeh Alatab, Soroosh Shekarchian, Iraj Najafi, Reza Moghadasali, Naser Ahmadbeigi, Mohammad Reza Pourmand, Tina Bolurieh, Neda Jaroughi, Gholamreza Pourmand, Nasser Aghdami
Objective: Using mesenchymal stem cells (MSCs) is regarded as a new therapeutic approach for improving fibrotic diseases. the aim of this study to evaluate the feasibility and safety of systemic infusion of autologous adipose tissue-derived MSCs (AD-MSCs) in peritoneal dialysis (PD) patients with expected peritoneal fibrosis. Materials and Methods: This study was a prospective, open-label, non-randomized, placebo-free, phase I clinical trial. Case group consisted of nine eligible renal failure patients with more than two years of history of being on PD...
January 2019: Cell Journal
Samaneh Karimi, Jafar Ai, Layasadat Khorsandi, Darioush Bijan Nejad, Ghasem Saki
Objective: Type 1 diabetes is caused by destruction of beta cells of pancreas. Vildagliptin (VG), a dipeptidyl peptidase IV (DPP IV) inhibitor, is an anti-diabetic drug, which increases beta cell mass. In the present study, the effects of VG on generation of insulin-producing cells (IPCs) from adipose-derived mesenchymal stem cells (ASCs) is investigated. Materials and Methods: In this experimental study, ASCs were isolated and after characterization were exposed to differentiation media with or without VG...
January 2019: Cell Journal
Mohammad Reza Mokhber Dezfouli, Sirous Sadeghian Chaleshtori, Azadeh Moradmand, Mohsen Basiri, Hossein Baharvand, Yaser Tahamtani
Objective: The ability to generate lung alveolar epithelial type II (ATII) cells from pluripotent stem cells (PSCs) enables the study of lung development, regenerative medicine, and modeling of lung diseases. The establishment of defined, scalable differentiation methods is a step toward this goal. This study intends to investigate the competency of small molecule induced mouse embryonic stem cell-derived definitive endoderm (mESC-DE) cells towards ATII cells. Materials and Methods: In this experimental study, we designed a two-step differentiation protocol...
January 2019: Cell Journal
Niloufar Shayan Asl, Farhad Nejat, Parvaneh Mohammadi, Abdolhossein Nekoukar, Saeed Hesam, Marzieh Ebrahimi, Khosrow Jadidi
Objective: Human amniotic membrane (HAM) is used as a supporter for limbal stem cell (LSC) expansion and corneal surgery. The aim of study is to use HAM extracts from healthy donors to enhance proliferation of LSCs in vitro and in vivo. Materials and Methods: In this interventional experimental study, the effective and cytotoxic doses of the amniotic membrane extract eye drops (AMEED) was assessed by adding different concentrations of AMEED (0-2.0 mg/ml) to LSC cultures for 14 days...
January 2019: Cell Journal
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