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Cell Journal

Farah Talebi, Farideh Ghanbari Mardasi, Javad Mohammadi Asl, Saeed Tizno, Marziye Najafvand Zadeh
Autosomal recessive non-syndromic hearing loss (ARNSHL) is defined as a genetically heterogeneous disorder. The aim of the present study was to screen for pathogenic variants in an Iranian pedigree with ARNSHL. Next-generation targeted sequencing of 127 deafness genes in the proband detected two novel variants, a homozygous missense variant in PTPRQ (c.2599 T>C, p.Ser867Pro and a heterozygous missense variant in MYO1A (c.2804 T>C, p.Ile935Thr), both of which were absent in unaffected sibs and two hundred unaffected controls...
April 2018: Cell Journal
Morteza Yaftian, Fatemeh Yari, Mehran Ghasemzadeh, Vahid Fallah Azad, Mansoureh Haghighi
OBJECTIVES: The in vitro treatment of tumor cells with platelet (Plt) causes inhibition of tumor cell growth, although mechanism of this effect is not clear yet. Induction of apoptosis has been proposed as a mechanism of Plt effects on tumor cells. The purpose of this study was to clarify the role of Plts and Plt-derived components in the induction of apoptosis in the blood mononuclear cells of patients with leukemia. MATERIALS AND METHODS: In this experimental study, peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of five patients with childhood B-precursor acute lymphoblastic leukemia (pre-B ALL) and encountered with Plts, Plt-derived microparticles (Plt-MPs) as well as purified soluble CD40L (sCD40L)...
April 2018: Cell Journal
Mahsa Rezaeepoor, Mazdak Ganjalikhani-Hakemi, Shima Shapoori, Nahid Eskandari, Mohammadreza Sharifi, Masoud Etemadifar, Marjan Mansuorian
OBJECTIVES: Semaphorin-3A (SEMA3A) and its receptors are found on some immune cells and act as suppressors of immune cells over-activation. Considering the role of SEMA3A and its down-regulation in some autoimmune diseases, as well as our bioinformatics predictions, we assumed that miR-145-5p might affect SEMA3A expression. So, we aimed to determine the effect of miR-145-5p on SEMA3A gene expression level. MATERIALS AND METHODS: In this experimental study, we evaluated the effect of miR-145-5p transfection on SEMA3A expression in peripheral blood mononuclear cells (PBMCs) using ELISA and quantitative real-time polymerase chain reaction (PCR) methods...
April 2018: Cell Journal
Maryam Ezzati, Leila Roshangar, Jafar Soleimani Rad, Nahid Karimian
OBJECTIVES: Infertility is a worldwide health problem which affects approximately 15% of sexually active couples. One of the factors influencing the fertility is melatonin. Also, protection of oocytes and embryos from oxidative stress inducing chemicals in the culture medium is important. The aim of the present study was to investigate if melatonin could regulate hyaluronan synthase-2 (HAS2) and Progesterone receptor (PGR) expressions in the cumulus cells of mice oocytes and provide an in vitro fertilization (IVF) approach...
April 2018: Cell Journal
Mohammad Hadi Sekhavati, Sayed Morteza Hosseini, Mojtaba Tahmoorespur, Kamran Ghaedi, Farnoosh Jafarpour, Mehdi Hajian, Kyanoosh Dormiani, Mohammad Hossain Nasr-Esfahani
OBJECTIVES: The Streptomyces phage phiC31 integrase offers a sequence-specific method of transgenesis with a robust long-term gene expression. PhiC31 has been successfully developed in a variety of tissues and organs for purpose of in vivo gene therapy. The objective of the present experiment was to evaluate PhiC31-based site-specific transgenesis system for production of transgenic bovine embryos by somatic cell nuclear transfer and intracytoplasmic sperm injection. MATERIALS AND METHODS: In this experimental study, the application of phiC31 integrase system was evaluated for generating transgenic bovine embryos by somatic cell nuclear transfer (SCNT) and sperm mediated gene transfer (SMGT) approaches...
April 2018: Cell Journal
Parvin Dorfeshan, Marefat Ghaffari Novin, Mohammad Salehi, Reza Masteri Farahani, Fatemeh Fadaei-Fathabadi, Ronak Sehatti
OBJECTIVES: In vitro maturation technique (IVM) is shown to have an effect on full maturation of immature oocytes and the subsequent embryo development. Embryonic genome activation (EGA) is considered as a crucial and the first process after fertilization. EGA failure leads to embryo arrest and possible implantation failure. This study aimed to determine the role of IVM in EGA-related genes expression in human embryo originated from immature oocytes and recovered from women receiving gonadotrophin treatment for assisted reproduction...
April 2018: Cell Journal
Oranous Bashti, Mehrdad Noruzinia, Masoud Garshasbi, Morteza Abtahi
OBJECTIVES: Endometriosis is a prevalent gynecologic disease affecting 10% of women in reproductive age. Endometriosis is diagnosed by laparoscopy that was followed by histologic confirmation. Early diagnosis will lead to a more effective treatment with much less morbidity. As miR-31 and miR-145 are shown to be directly or indirectly correlated to biological processes involved in endometriosis, the aim of this study was to examine the association of miR-31 and miR-145 expression in plasma with the presence of endometriosis...
April 2018: Cell Journal
Hossein Eslami, Ali Eslami, Raha Favaedi, Ummolbanin Asadpour, Shabnam Zari Moradi, Poopak Eftekhari-Yazdi, Tahereh Madani, Maryam Shahhoseini, Anahita Mohseni Meybodi
OBJECTIVES: The diminished ovarian reserve (DOR) is a condition characterized by a reduction in the number and/or quality of oocytes. This primary infertility disorder is usually accompanied with an increase in the follicle-stimulating hormone (FSH) levels and regular menses. Although there are many factors contributing to the DOR situation, it is likely that many of idiopathic cases have genetic/epigenetic bases. The association between the FMR1 premutation (50-200 CGG repeats) and the premature ovarian failure (POF) suggests that epigenetic disorders of FMR1 can act as a risk factor for the DOR as well...
April 2018: Cell Journal
Fahimeh Mollaahmadi, Ashraf Moini, Reza Salman Yazdi, Mehrdad Behmanesh
OBJECTIVES: Infertility is a common human disorder which is defined as the failure to conceive for a period of 12 months without contraception. Many studies have shown that the outcome of fertility could be affected by DNA damage. We attempted to examine the association of two SNPs (rs1127354 and rs7270101) in ITPA, a gene encoding a key factor in the repair system, with susceptibility to infertility. MATERIALS AND METHODS: This was a case-control study of individuals with established infertility...
April 2018: Cell Journal
Sharif Moradi, Thomas Braun, Hossein Baharvand
OBJECTIVES: Embryonic stem cells (ESCs) are regulated by a gene regulatory circuitry composed of transcription factors, signaling pathways, metabolic mediators, and non-coding RNAs (ncRNAs). MicroRNAs (miRNAs) are short ncRNAs which play crucial roles in ESCs. Here, we explored the impact of miR-302b-3p on ESC self-renewal in the absence of leukemia inhibitory factor (LIF). MATERIALS AND METHODS: In this experimental study, ESCs were cultured in the presence of 15% fetal bovine serum (FBS) and induced to differentiate by LIF removal...
April 2018: Cell Journal
Tae Woo Kim, Myung Chul Lee, Hyun Cheol Bae, Hyuk-Soo Han
OBJECTIVES: Coculture of chondrocytes and mesenchymal stem cells (MSCs) has been developed as a strategy to overcome the dedifferentiation of chondrocytes during in vitro expansion in autologous chondrocyte transplantation. Synovium-derived stem cells (SDSCs) can be a promising cell source for coculture due to their superior chondrogenic potential compared to other MSCs and easy accessibility without donor site morbidity. However, studies on coculture of chondrocytes and SDSCs are very limited...
April 2018: Cell Journal
Shahnaz Razavi, Nazem Ghasemi, Mohammad Mardani, Hossein Salehi
OBJECTIVES: The presence of neurotrophic factors is critical for regeneration of neural lesions. Here, we transplanted combination of neurotrophic factor secreting cells (NTF-SCs) and human adipose derived stem cells (hADSCs) into a lysolecithin model of multiple sclerosis (MS) and determined the myelinization efficiency of these cells. MATERIALS AND METHODS: In this experimental study, 50 adult rats were randomly divided into five groups: control, lysolecithin, vehicle, hADSCs transplantation and NTF-SCs/ hADSCs co-transplantation group...
April 2018: Cell Journal
Esmaeil Rahimi, Amirhossein Ahmadi, Mohammad Ali Boroumand, Bahram Mohammad Soltani, Mehrdad Behmanesh
OBJECTIVES: ANRIL is an important antisense noncoding RNA gene in the INK4 locus (9p21.3), a hot spot region associated with multiple disorders including coronary artery disease (CAD), type 2 diabetes mellitus (T2DM) and many different types of cancer. It has been shown that its expression is dysregulated in a variety of immune-mediated diseases. CAD is a major problem in T2DM patients and the cause of almost 60% of deaths in these patients worldwide. The aim of the present study was to compare the expression level of ANRIL between T2DM patients with and without CAD...
April 2018: Cell Journal
Naeem Erfani Majd, Mohammad Reza Tabandeh, Ali Shahriari, Zahra Soleimani
OBJECTIVES: Okra (Abelmoschus esculentus) is a tropical vegetable that is rich in carbohydrates, fibers, proteins and natural antioxidants. The aim of the present study was to evaluate the effects of Okra powder on pancreatic islets and its action on the expression of PPAR-γ and PPAR-α genes in pancreas of high-fat diet (HFD) and streptozotocininduced diabetic rats. MATERIALS AND METHODS: In this experimental study, diabetes was induced by feeding HFD (60% fat) for 30 days followed by an injection of streptozotocin (STZ, 35 mg/kg)...
April 2018: Cell Journal
Ahad Khoshzaban, Peyman Keyhanvar, Elham Delrish, Farhood Najafi, Saeed Heidari Keshel, Ikuya Watanabe, Alireza Valanezhad, Tahereh Jafarzadeh Kashi
OBJECTIVES: Alginate, known as a group of anionic polysaccharides extracted from seaweeds, has attracted the attention of researchers because of its biocompatibility and degradability properties. Alginate has shown beneficial effects on wound healing as it has similar function as extracellular matrix. Alginate microcapsules (AM) that are used in tissue engineering as well as Dulbecco's modified Eagle's medium (DMEM) contain nutrients required for cell viability. The purpose of this research was introducing AM in medium and nutrient reagent cells and making a comparison with control group cells that have been normally cultured in long term...
April 2018: Cell Journal
Maryam Mohammadi Najafabadi, Karim Shamsasenjan, Parvin Akbarzadehlaleh
OBJECTIVES: Angiogenesis, the process of formation of new blood vessels, is essential for development of solid tumors. At first, it was first assumed that angiogenesis is not implicated in the development of acute myeloid leukemia (AML) as a liquid tumor. One of the most important elements in bone marrow microenvironment is mesenchymal stem cells (MSCs). These cells possess an intrinsic tropism for sites of tumor in various types of cancers and have an impact on solid tumors growth by affecting the angiogenic process...
April 2018: Cell Journal
Nasrin Khanmohammadi, Hamid Reza Sameni, Moslem Mohammadi, Abbas Pakdel, Majid Mirmohammadkhani, Houman Parsaie, Sam Zarbakhsh
OBJECTIVES: Although stem cell transplantation has beneficial effects on tissue regeneration, but there are still problems such as high cost and safety issues. Since stem cell therapy is largely dependent on paracrine activity, in this study, utilization of transplantation of bone marrow stromal cells (BMSCs)-secretome instead of the cells, into damaged ovaries was evaluated to overcome the limitations of stem cell transplantation. MATERIALS AND METHODS: In this experimental study, BMSCs were cultured and 25-fold concentrated conditioned medium (CM) from BMSCs was prepared...
April 2018: Cell Journal
Hajar Estiri, Ali Fallah, Masoud Soleimani, Abbas Aliaghaei, Fariba Karimzadeh, Shahnaz Babaei Abraki, Mohammad Hossein Ghahremani
OBJECTIVES: In this study, we describe an efficient approach for stable knockdown of adenosine kinase (ADK) using lentiviral system, in an astrocytoma cell line and in human Wharton's jelly mesenchymal stem cells (hWJMSCs). These sources of stem cells besides having multilineage differentiation potential and immunomodulatory activities, are easily available in unlimited numbers, do not raise ethical concerns and are attractive for gene manipulation and cell-based gene therapy. MATERIALS AND METHODS: In this experimental study, we targeted adenosine kinase mRNA at 3' and performed coding sequences using eight miR-based expressing cassettes of anti-ADK short hairpin RNA (shRNAs)...
April 2018: Cell Journal
Zahra Eelaminejad, Raha Favaedi, Tahereh Modarresi, Marjan Sabbaghian, Mohammad Ali Sadighi Gilani, Maryam Shahhoseini
Identification of molecular markers which can predict the outcome of sperm retrieval non-invasively in patients with non-obstructive azoospermia (NOA) are valuable in clinical andrology. Jumonji domain-containing 1a (JMJD1A) is a significant epigenetic regulator during spermatogenesis, which plays an important role in the differentiation of post-meiotic germ cells into mature spermatozoa. We therefore aimed to examine the potential association between JMJD1A expression and the outcome of sperm retrieval in patients with NOA...
January 2018: Cell Journal
Tahereh Foroutan, Aisan Farhadi, Saeed Abroun, Bahram Mohammad Soltani
OBJECTIVES: Umbilical cord blood is used for transplantation purposes in regenerative medicine of hematological disorders. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as cancer development and incidence. There is a new relation between p53 (tumor suppressor gene) and miR-145 (suppressor of cell growth) upregulation. In this study, we have assessed how adipose-derived stem cells (ADSCs) affect the expansion of hematopoietic stem cells (HSCs), as well as miR-145 and p53 expressions...
January 2018: Cell Journal
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