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Iranian Journal of Basic Medical Sciences

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https://www.readbyqxmd.com/read/28293400/gestational-diabetes-influences-retinal-muller-cells-in-rat-s-offspring
#1
Akramsadat Tabasi, Soraya Ghafari, Mehdi Mehdizadeh, Majid Asadi Shekari, Mohammad Jafar Golalipour
OBJECTIVES: The Muller cell is the principal glial cell of the vertebrate retina. The expression of Glial fibrillary acidic protein (GFAP) in the Muller cells was used as a cellular marker for retinal damage. This study was done to evaluate the effect of gestational diabetes on retinal Muller cells in rat's offspring. MATERIALS AND METHODS: In this experimental study, 12 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293399/up-regulation-of-tlr2-and-tlr4-in-high-mobility-group-box1-stimulated-macrophages-in-pulpitis-patients
#2
Javad Mahmoudi, Babak Sabermarouf, Behzad Baradaran, Leila Sadat-Hatamnezhad, Siamak Sandoghchian Shotorbani
OBJECTIVES: High Mobility Group Box1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in a variety of proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can up-regulate the expression of Toll-like Receptor 2 (TLR2) and Toll-like Receptor 4 (TLR4) on macrophages from pulpitis and to clarify the subsequent events involving Th17 cells and Th17 cell-associated cytokine changes...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293398/nicotine-induced-damages-in-testicular-tissue-of-rats-evidences-for-bcl-2-p53-and-caspase-3-expression
#3
Maryam Mosadegh, Shapour Hasanzadeh, Mazdak Razi
OBJECTIVES: Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. MATERIALS AND METHODS: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW(-1), IP) nicotine-received and high dose (0.4 mg/kg BW(-1), IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293397/in-vitro-lymphoproliferative-response-and-cytokine-production-in-mice-with-experimental-disseminated-candidiasis
#4
Ali Reza Khosravi, Hojjatollah Shokri, Shahin Eshghi
OBJECTIVES: Systemic candidiasis is an infection of Candida albicans (C. albicans) causing disseminated disease and sepsis, invariably when host defenses are compromised. We investigated the histopathological changes as well as the lymphoproliferative responses and cytokine production of splenic cells after stimulation with Concanavalin A (Con A) and Pokeweed mitogen (PWM) in mice with disseminated candidiasis. MATERIALS AND METHODS: Lymphoproliferative responses were stimulated in vitro with Con A (1 µg/ml) and PWM (1 µg/ml) mitogens in Roswell Park Memorial Institute (RPMI) 1640 media, and the production of interferon (IFN)-γ and interleukin-4 (IL-4) in the supernatants was measured by enzyme-linked immunosorbent assay (ELISA)...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293396/the-mir-383-ldha-axis-regulates-cell-proliferation-invasion-and-glycolysis-in-hepatocellular-cancer
#5
Zhixiong Fang, Langqiu He, Hui Jia, Qiusheng Huang, Dan Chen, Zhiwei Zhang
OBJECTIVES: To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). MATERIALS AND METHODS: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate production assay to explore the function of miR-383 in cell proliferation, invasion and glycolysis in HCC cell lines...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293395/expression-pattern-of-neurotrophins-and-their-receptors-during-neuronal-differentiation-of-adipose-derived-stem-cells-in-simulated-microgravity-condition
#6
Vajiheh Zarrinpour, Zahra Hajebrahimi, Mojtaba Jafarinia
OBJECTIVES: Studies have confirmed that microgravity, as a mechanical factor, influences both differentiation and function of mesenchymal stem cells. Here we investigated the effects of simulated microgravity on neural differentiation of human adipose-derived stem cells (ADSCs). MATERIALS AND METHODS: We have used a fast rotating clinostat (clinorotation) to simulate microgravity condition. Real-time PCR and flow cytometry analysis were used to evaluate the regulation of neurotrophins, their receptors, and neural markers by simulated microgravity and their impact on neural differentiation of cells...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293394/evaluation-of-the-expression-of-viii-factor-and-vegf-in-the-regeneration-of-non-vital-teeth-in-dogs-using-propolis
#7
Mina Zarei, Amir Hossein Jafarian, Azadeh Harandi, Maryam Javidi, Maryam Gharechahi
OBJECTIVES: The purpose of the present study was the immunohistochemical evaluation of VEGF and VII factors in dog's teeth pulp revascularized with MTA and propolis. MATERIALS AND METHODS: 144 mature and immature two rooted dog's premolar canals were selected. Pulp necrosis and infection were established after 2 weeks and the disinfection of the canals was done with copious NaOCl irrigation and triantibiotic mixture (ciprofloxacin, metronidazole, and minocycline) for 3 weeks...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293393/cytotoxic-and-apoptotic-effects-of-different-extracts-of-artemisia-biennis-willd-on-k562-and-hl-60-cell-lines
#8
Zahra Tayarani-Najaran, Farideh-Sadat Makki, Nafiseh-Sadat Alamolhodaei, Mahdi Mojarrab, Seyed Ahmad Emami
OBJECTIVES: Artemisia is a genus of herbs and small shrubs forms an important part of natural vegetation in Iran. It has been reported that several Artemisia species possess anti-proliferative effects. Considering the value of this genus in anti-cancer researches we have chosen Artemisia biennis for cytotoxic and mechanistic studies. MATERIALS AND METHODS: In this study we have investigated the cytotoxic and apoptotic effects of petroleum ether, dichloromethane, ethyl acetate, ethanol, and ethanol: water (1:1 v/v) extracts of A...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293392/the-effect-of-hydro-ethanolic-extract-of-curcuma-longa-rhizome-and-curcumin-on-total-and-differential-wbc-and-serum-oxidant-antioxidant-biomarkers-in-rat-model-of-asthma
#9
Farzaneh Shakeri, Mohammad Soukhtanloo, Mohammad Hossein Boskabady
OBJECTIVES: The effects of Curcuma longa (C. longa) and curcumin on total and differential WBC count and oxidant, antioxidant biomarkers, in rat model of asthma were evaluated. MATERIALS AND METHODS: Total and differential WBC count in the blood, NO2, NO3, MDA, SOD, CAT and thiol levels in serum were examined in control, asthma, Asthmatic rats treated with C. longa (0.75, 1.50, and 3.00 mg/ml), curcumin (0.15, 0.30, and 0.60 mg/ml), and dexamethasone (1.25 μg/ml) rats...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293391/gestational-diabetes-leads-to-down-regulation-of-cdk4-prb-e2f1-pathway-genes-in-pancreatic-islets-of-rat-offspring
#10
Zahra Nazari, Mohammad Nabiuni, Mohsen Saeidi, Mohammad Jafar Golalipour
OBJECTIVES: The link between a hyperglycemic intrauterine environment and the development of diabetes later in life has been observed in offspring exposed to gestational diabetes mellitus (GDM), but the underlying mechanisms for this phenomenon are still not clear. Reduced β-cells mass is a determinant in the development of diabetes (type 1 and type 2 diabetes). Some recent studies have provided evidence that the CDK4-pRB-E2F1 regulatory pathway is involved in β-cells proliferation...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293390/effect-of-sodium-hydrosulfide-on-mrna-expression-of-prostaglandin-e2-receptors-in-response-to-mucosal-acidification-and-distention-induced-gastric-acid-secretion-in-rats
#11
Seyyed Ali Mard, Simin Mahini, Mahin Dianat, Yaghoob Farbood
OBJECTIVES: Prostaglandins have been shown to mediate the gastro-protective effect of sodium hydrosulfide (NaHS) but effect of NaHS on mRNA expression of prostaglandin E2 receptors (EP1, 3-4; EPs) has not been investigated. Therefore, this study designed to evaluate the effect of NaHS on mRNA expression of EPs receptors in response to mucosal acidification and distention-induced gastric acid secretion in rats. MATERIALS AND METHODS: Fasted rats were randomly assigned into 4 groups (n=6/group)...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293389/exploring-the-effect-of-intravenous-lipid-emulsion-in-acute-methamphetamine-toxicity
#12
Ameneh Ghadiri, Leila Etemad, Mohammad Moshiri, Seyed Adel Moallem, Amir Hossein Jafarian, Farzin Hadizadeh, Mahmoud Seifi
OBJECTIVES: The increasing use of methamphetamine (METH) in the last decades has made it the second most abused drug. Advancs in the area of intravenous lipid emulsion (ILE) have led to its potential application in the treatment of poisoning. The present study aims to investigate the potential role of ILE as an antidote for acute METH poisoning. MATERIALS AND METHODS: Two groups of six male rats were treated by METH (45 mg/kg), intraperitoneally. Five to seven min later, they received an infusion of 18...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293388/protective-role-of-licochalcone-b-against-ethanol-induced-hepatotoxicity-through-regulation-of-erk-signaling
#13
Xiao-Peng Gao, Dong-Wei Qian, Zhen Xie, Hao Hui
OBJECTIVES: Oxidative stress has been established as a key cause of alcohol-induced hepatotoxicity. Licochalcone B, an extract of licorice root, has shown antioxidative properties. This study was to investigate the effects and mechanisms of licochalcone B in ethanol-induced hepatic injury in an in vitro study. MATERIALS AND METHODS: An in vitro model of Ethanol-induced cytotoxicity in BRL cells was used in this study. Cell injury was assessed using WST-1 assay and lactate dehydrogenase, alanine transaminase, and aspartate aminotransferase release assay...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293387/cfp10-mfc%C3%AE-2-as-a-novel-tuberculosis-vaccine-candidate-increases-immune-response-in-mouse
#14
Ali Asghar Baghani, Saman Soleimanpour, Hadi Farsiani, Arman Mosavat, Masoud Yousefi, Zahra Meshkat, Seyed Abdolrahim Rezaee, Saeid Amel Jamehdar, Mohammad Reza Akbari Eydgahi, Hamid Sadeghian, Kiarash Ghazvini
OBJECTIVES: Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-domain of mouse IgG2a was evaluated as a novel subunit vaccine candidate against TB. MATERIALS AND METHODS: The genetic constructs were cloned in pPICZαA expression vector and recombinant vectors (pPICZαA-CFP-10: Fcγ2a and pPICZαA-CFP-10:His) were transformed into Pichia pastoris...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293386/toxicology-effects-of-saffron-and-its-constituents-a-review
#15
REVIEW
Hasan Badie Bostan, Soghra Mehri, Hossein Hosseinzadeh
Saffron (Crocus sativus L.) has been considered as a medicinal plant since ancient times and also widely used as food additive for its color, taste and odor. The pharmacological properties of saffron and its main constituents, crocin and safranal have been evaluated using different in vivo and in vitro models. Additionally, other lines of studies have found toxicological effects of saffron. However, a comprehensive review that covers all aspects of its toxicity has not been published yet. The current study provides classified information about the toxic effects of saffron and its constituents in various exposure conditions including acute, sub-acute, sub-chronic and chronic studies...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293385/adiporon-may-be-benefit-for-atherosclerosis-prevention
#16
EDITORIAL
Maryam Esfahani, Nooshin Shabab, Massoud Saidijam
Atherosclerosis has serious role in coronary arteries disease, so it is important to establish effective strategies for prevention or even treatment of atherosclerosis. Adiponectin, as one of the most abundant adipokines, has insulin sensitivity, anti-inflammatory and anti-atherogenic properties. Disturbed adiponectin actions through its receptor, (AdipoR1 and AdipoR2) may be involved in atherosclerosis development. Some adiponectin effects are mediated by AMPK and PPAR-α signaling. AdipoRon is an orally active synthetic molecule which can bind to AdipoR1, Adipo R2 and activate them...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28133531/in-vitro-assesment-of-anti-inflammatory-activities-of-coumarin-and-indonesian-cassia-extract-in-raw264-7-murine-macrophage-cell-line
#17
Ni Made Dwi Sandhiutami, Moordiani Moordiani, Dian Ratih Laksmitawati, Nurul Fauziah, Maesaroh Maesaroh, Wahyu Widowati
OBJECTIVES: Inflammation is an immune response toward injuries. Although inflammation is healing response, but in some condition it will lead to chronic disease such as rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, Alzheimer's and various cancer. Indonesian cassia (Cinnamomum burmannil C. Nees & T. Ness) known to contain coumarin, is widely used for alternative medicine especially as an anti-inflammatory. This study was conducted to determine the anti-inflammatory properties of coumarin and Indonesian cassia extract (ICE) in LPS-induced RAW264...
January 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28133530/endurance-training-induces-fiber-type-specific-revascularization-in-hindlimb-skeletal-muscles-of-rats-with-chronic-heart-failure
#18
Kamal Ranjbar, Malihe Ardakanizade, Farzad Nazem
OBJECTIVES: Previous studies showed that skeletal muscle microcirculation was reduced in chronic heart failure. The aim of this study was to investigate the effects of endurance training on capillary and arteriolar density of fast and slow twitch muscles in rats with chronic heart failure. MATERIALS AND METHODS: Four weeks after surgeries (left anterior descending (LAD) artery occlusion), chronic heart failure rats were divided into 3 groups: Sham (Sham, n=10); Sedentary (Sed, n=10); Exercise training (Ex, n=10)...
January 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28133529/cisplatin-cytotoxicity-is-dependent-on-mitochondrial-respiration-in-saccharomyces-cerevisiae
#19
Santhi Priya Inapurapu, Karunakar Rao Kudle, Sreedhar Bodiga, Vijaya Lakshmi Bodiga
OBJECTIVES: To understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial DNA depleted Rho0 yeast cells. MATERIALS AND METHODS: Wild type and Rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, pH changes in cytosol/mitochondrial compartments, reactive oxygen species production and respiratory control ratio...
January 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28133528/effects-of-ellagic-acid-pretreatment-on-renal-functions-disturbances-induced-by-global-cerebral-ischemic-reperfusion-in-rat
#20
Khojasteh Hoseiny Nejad, Mohammad Kazem Gharib-Naseri, Alireza Sarkaki, Mahin Dianat, Mohammad Badavi, Yaghoub Farbood
OBJECTIVES: Global cerebral ischemia-reperfusion (GCIR) causes disturbances in brain functions as well as other organs such as kidney. Our aim was to evaluate the protective effects of ellagic acid (EA) on certain renal disfunction after GCIR. MATERIALS AND METHODS: Adult male Wistar rats (n=32, 250-300 g) were used. GCIR was induced by bilateral vertebral and common carotid arteries occlusion (4-VO). Animal groups were: 1) received DMSO/saline (10%) as solvent of EA, 2) solvent + GCIR, 3) EA + GCIR, and 4) EA...
January 2017: Iranian Journal of Basic Medical Sciences
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