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Iranian Journal of Basic Medical Sciences

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https://www.readbyqxmd.com/read/28392907/protective-effect-of-crocus-sativus-l-saffron-extract-on-spinal-cord-ischemia-reperfusion-injury-in-rats
#1
Gholam Hossein Farjah, Shadi Salehi, Mohammad Hasan Ansari, Bagher Pourheidar
OBJECTIVES: Ischemia/reperfusion (I/R) injury of spinal cord is leading to the paraplegia observed. In this study, we investigated the protective effect of the saffron extract on spinal cord I/R injury. MATERIALS AND METHODS: Thirty five male Sprague-Dawley rats were divided into 5 groups: intact, sham surgery, normal saline (NS), low dose saffron aqua extract, high dose saffron aqua extract. RESULTS: The mean motor deficit index (MDI) scores were significantly lower in the saffron extract groups than in the NS group at 48 hr after spinal cord ischemia (P<0...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392906/selection-of-single-chain-variable-fragments-specific-for-mycobacterium-tuberculosis-esat-6-antigen-using-ribosome-display
#2
Shahrzad Ahangarzadeh, Mojgan Bandehpour, Bahram Kazemi
OBJECTIVES: Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. MATERIALS AND METHODS: The gene encoding ESAT-6 was cloned into pET22b(+) plasmid and expressed in Escherichia coli BL21 (DE3)...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392905/cinnamaldehyde-and-eugenol-change-the-expression-folds-of-akt1-and-dkc1-genes-and-decrease-the-telomere-length-of-human-adipose-derived-stem-cells-hascs-an-experimental-and-in-silico-study
#3
Abdorrahim Absalan, Seyed Alireza Mesbah-Namin, Taki Tiraihi, Taher Taheri
OBJECTIVES: To investigate the effect of cinnamaldehyde and eugenol on the telomere-dependent senescence of stem cells. In addition, to search the probable targets of mentioned phytochemicals between human telomere interacting proteins (TIPs) using in silico studies. MATERIALS AND METHODS: Human adipose derived stem cells (hASCs) were studied under treatments with 2.5 µM/ml cinnamaldehyde, 0.1 µg/ml eugenol, 0.01% DMSO or any additive. The expression of TERT, AKT1 and DKC1 genes and the telomere length were assessed over 48-hr treatment...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392904/protective-effects-of-tanshinone-iia-sodium-sulfonate-on-ischemia-reperfusion-induced-myocardial-injury-in-rats
#4
Yun Pan, Jin-Xian Qian, Shi-Qi Lu, Jing-Wei Chen, Xiao-Dong Zhao, Yan Jiang, Lin-Hui Wang, Guo-Xing Zhang
OBJECTIVES: This study investigated the protective effect of tanshinone IIA sodium sulfonate (TSS) on ischemia-reperfusion (I/R) induced cardiac injury, and the underlying mechanism of action. MATERIALS AND METHODS: Male Sprague-Dawley rats were subjected to a 30-min coronary arterial occlusion followed by 24 hours' reperfusion. Half an hour before the left coronary artery ligation, rats were pretreated with TSS in three different dosages (15, 30, 70 mg/kg, IP)...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392903/emergence-of-signs-of-neural-cells-after-exposure-of-bone-marrow-derived-mesenchymal-stem-cells-to-fetal-brain-extract
#5
Iman Razeghian Jahromi, Davood Mehrabani, Ali Mohammadi, Mohammad Mahdi Ghahramani Seno, Mehdi Dianatpour, Shahrokh Zare, Amin Tamadon
OBJECTIVES: Nowadays much effort is being invested in order to diagnose the mechanisms involved in neural differentiation. By clarifying this, making desired neural cells in vitro and applying them into diverse neurological disorders suffered from neural cell malfunctions could be a feasible choice. Thus, the present study assessed the capability of fetal brain extract (FBE) to induce rat bone marrow-derived mesenchymal stem cells (BM-MSCs) toward neural cells. MATERIALS AND METHODS: For this purpose, BM-MSCs were collected from rats and cultured and their mesenchymal properties were confirmed...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392902/detection-of-th17-treg-cells-and-related-factors-in-gingival-tissues-and-peripheral-blood-of-rats-with-experimental-periodontitis
#6
Li Gao, Yajing Zhao, Panpan Wang, Liping Zhang, Chi Zhang, Qianying Chen, Chuanjiang Zhao
OBJECTIVES: This study aimed to investigate the role and the possible mechanisms involved in the immunoregulation of experimental periodontitis by Th17/Treg. MATERIALS AND METHODS: Experimental periodontitis was established by silk thread ligation with Porphyromonas gingivalis daubing in the bilateral maxillary second molar of Male Sprague-Dawley (SD) rats. Alveolar bones were scanned by Micro-CT. Histological examination was stained with H&E. The proportions of Th17 and Treg cells in peripheral blood were detected by flow cytometry...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392901/mutation-analysis-of-connexin-50-gene-among-iranian-families-with-autosomal-dominant-cataracts
#7
Masoumeh Mohebi, Saeed Chenari, Abolfazl Akbari, Fariba Ghassemi, Mehran Zarei-Ghanavati, Ghasem Fakhraie, Nahid Babaie, Mansour Heidari
OBJECTIVES: Childhood cataract is a genetically heterogeneous eye disorder that results in visual impairment. The aim of this study was to identify the genetic mutations of connexin 50 gene among Iranian families suffered from autosomal dominant congenital cataracts (ADCC). MATERIALS AND METHODS: Families, having at least two members with bilateral familial congenital cataract, were selected for the study. Probands were evaluated by detailed ophthalmologist's examination, and the pedigree analysis was performed...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392900/cadmium-chloride-treatment-of-rats-significantly-impairs-membrane-integrity-of-mesenchymal-stem-cells-via-electrolyte-imbalance-and-lipid-peroxidation-a-possible-explanation-of-cd-related-osteoporosis
#8
Mohammad Husein Abnosi, Someyeh Golami
OBJECTIVES: Bone marrow mesenchymal stem cells (MSCs) play an important role in bone health. Cadmium causes osteoporosis, but the exact mechanisms of its effect on MSCs are not known. MATERIALS AND METHODS: Rats were treated with cadmium chloride (40 mg/l) in drinking water for six weeks, and then the biochemical and morphological studies on MSCs were carried out as a cellular backup for osteoblasts. Viability and proliferation properties of the cells were evaluated using MTT assay, trypan blue, population doubling number, and colony forming assay...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392899/scutellarin-may-alleviate-cognitive-deficits-in-a-mouse-model-of-hypoxia-by-promoting-proliferation-and-neuronal-differentiation-of-neural-stem-cells
#9
Wei-Wei Wang, Jian-Hong Han, Lin Wang, Tian-Hao Bao
OBJECTIVES: Scutellarin, a flavonoid extracted from the medicinal herb Erigeron breviscapus Hand-Mazz, protects neurons from damage and inhibits glial activation. Here we examined whether scutellarin may also protect neurons from hypoxia-induced damage. MATERIALS AND METHODS: Mice were exposed to hypoxia for 7 days and then administered scutellarin (50 mg/kg/d) or vehicle for 30 days Cognitive impairment in the two groups was assessed using the Morris water maze test, cell proliferation in the hippocampus was compared using 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry, and hippocampal levels of nestin and neuronal class III β-tubulin (Tuj-1) were measured using Western blotting...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392898/comparative-proteome-analysis-of-human-esophageal-cancer-and-adjacent-normal-tissues
#10
Rezvan Yazdian-Robati, Homa Ahmadi, Maryam Matbou Riahi, Parisa Lari, Seyed Amir Aledavood, Marzieh Rashedinia, Khalil Abnous, Mohammad Ramezani
OBJECTIVES: Ranking as the sixth commonest cancer, esophageal squamous cell carcinoma (ESCC) represents one of the leading causes of cancer death worldwide. One of the main reasons for the low survival of patients with esophageal cancer is its late diagnosis. MATERIALS AND METHODS: We used proteomics approach to analyze ESCC tissues with the aim of a better understanding of the malignant mechanism and searching candidate protein biomarkers for early diagnosis of esophageal cancer...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392897/the-effect-of-caffeine-on-orthodontic-tooth-movement-in-rats
#11
Mohsen Shirazi, Hamed Vaziri, Behzad Salari, Pouria Motahhari, Shahroo Etemad-Moghadam, Ahmad Reza Dehpour
OBJECTIVES: to determine the effect of different doses of caffeine on orthodontic tooth movement (OTM) in rats. MATERIALS AND METHODS: Forty male 250-300 g Sprague-Dawley rats were randomly divided into four groups of ten animals each and received 0 (control), 1 g/l, 2 g/l and 3 g/l caffeine in tap water for 3 days. Orthodontic appliances were ligated between the maxillary first molars and incisors on the 4th day of the study period. All rats were sacrificed after 2 weeks of treatment after which OTM was measured...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392896/injury-to-skeletal-muscle-of-mice-following-acute-and-sub-acute-pregabalin-exposure
#12
Mohammad Moshiri, Seyed Adel Moallem, Armin Attaranzadeh, Zahra Saberi, Leila Etemad
OBJECTIVES: Pregabalin (PGB) is a new antiepileptic drug that has received FDA approval for patient who suffers from central neuropathic pain, partial seizures, generalized anxiety disorder, fibromyalgia and sleep disorders. This study was undertaken to evaluate the possible adverse effects of PGB on the muscular system of mice. MATERIALS AND METHODS: To evaluate the effect of PGB on skeletal muscle, the animals were exposed to a single dose of 1, 2 or 5 g /kg or daily doses of 20, 40 or 80 mg/kg for 21 days, intraperitoneally (IP)...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392895/association-between-the-synonymous-variant-organic-cation-transporter-3-oct3-1233g-a-and-the-glycemic-response-following-metformin-therapy-in-patients-with-type-2-diabetes
#13
Seyyedeh Raheleh Hosseyni-Talei, Abdolkarim Mahrooz, Mohammad Bagher Hashemi-Soteh, Maryam Ghaffari-Cherati, Ahad Alizadeh
OBJECTIVES: Organic cation transporter 3 (OCT3) as a high-capacity transporter contribute to the metabolism of metformin. The present study was conducted to determine the genotype frequencies of the variant OCT3-1233G>A (rs2292334) in patients with newly diagnosed type 2 diabetes (T2D) and its relationship with response to metformin. MATERIALS AND METHODS: This study included 150 patients with T2D who were classified into two groups following three months of metformin therapy: responders (by more than 1% reduction in HbA1c from baseline) and nonresponders (less than 1% reduction in HbA1c from baseline)...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392894/anti-melanogenic-activity-of-viola-odorata-different-extracts-on-b16f10-murine-melanoma-cells
#14
Vafa Baradaran Rahimi, Vahid Reza Askari, Seyed Ahmad Emami, Zahra Tayarani-Najaran
OBJECTIVES: In previous studies, antioxidant activity of Viola odorata L. has been demonstrated. In this study, we have investigated the anti-melanogenic effect of extract and fractions of the plant in B16F10 cell line. MATERIALS AND METHODS: Impact of different increasing concentrations of extract and fractions of V. odorata was evaluated on cell viability, cellular tyrosinase, melanin content and mushroom tyrosinase as well as ROS production in B16F10 murine melanoma cell line...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28392893/traditional-persian-topical-medications-for-gastrointestinal-diseases
#15
REVIEW
Laleh Dehghani Tafti, Seyyed Mahyar Shariatpanahi, Mahmoud Mahdavi Damghani, Behjat Javadi
Drug delivery across the skin is used for several millennia to ease gastrointestinal (GI) ailments in Traditional Persian Medicine (TPM). TPM topical remedies are generally being applied on the stomach, lower abdomen, lower back and liver to alleviate GI illnesses such as dyspepsia, gastritis, GI ulcers, inflammatory bowel disease, intestinal worms and infections. The aim of the present study is to survey the topical GI remedies and plant species used as ingredients for these remedies in TPM. In addition, pharmacological activities of the mentioned plants have been discussed...
March 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293400/gestational-diabetes-influences-retinal-muller-cells-in-rat-s-offspring
#16
Akramsadat Tabasi, Soraya Ghafari, Mehdi Mehdizadeh, Majid Asadi Shekari, Mohammad Jafar Golalipour
OBJECTIVES: The Muller cell is the principal glial cell of the vertebrate retina. The expression of Glial fibrillary acidic protein (GFAP) in the Muller cells was used as a cellular marker for retinal damage. This study was done to evaluate the effect of gestational diabetes on retinal Muller cells in rat's offspring. MATERIALS AND METHODS: In this experimental study, 12 Wistar rat dams were randomly allocated in control and diabetic groups. Gestational diabetes was induced by 40 mg/kg/body weight of streptozotocin at the first day of gestation, intraperitoneally...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293399/up-regulation-of-tlr2-and-tlr4-in-high-mobility-group-box1-stimulated-macrophages-in-pulpitis-patients
#17
Javad Mahmoudi, Babak Sabermarouf, Behzad Baradaran, Leila Sadat-Hatamnezhad, Siamak Sandoghchian Shotorbani
OBJECTIVES: High Mobility Group Box1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in a variety of proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can up-regulate the expression of Toll-like Receptor 2 (TLR2) and Toll-like Receptor 4 (TLR4) on macrophages from pulpitis and to clarify the subsequent events involving Th17 cells and Th17 cell-associated cytokine changes...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293398/nicotine-induced-damages-in-testicular-tissue-of-rats-evidences-for-bcl-2-p53-and-caspase-3-expression
#18
Maryam Mosadegh, Shapour Hasanzadeh, Mazdak Razi
OBJECTIVES: Present study was performed in order to uncover new aspects for nicotine-induced damages on spermatogenesis cell lineage. MATERIALS AND METHODS: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0.2 ml, saline normal, IP), low dose (0.2 mg/kg BW(-1), IP) nicotine-received and high dose (0.4 mg/kg BW(-1), IP) nicotine-received groups. Following 7 weeks, the expression of bcl-2, p53 and caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293397/in-vitro-lymphoproliferative-response-and-cytokine-production-in-mice-with-experimental-disseminated-candidiasis
#19
Ali Reza Khosravi, Hojjatollah Shokri, Shahin Eshghi
OBJECTIVES: Systemic candidiasis is an infection of Candida albicans (C. albicans) causing disseminated disease and sepsis, invariably when host defenses are compromised. We investigated the histopathological changes as well as the lymphoproliferative responses and cytokine production of splenic cells after stimulation with Concanavalin A (Con A) and Pokeweed mitogen (PWM) in mice with disseminated candidiasis. MATERIALS AND METHODS: Lymphoproliferative responses were stimulated in vitro with Con A (1 µg/ml) and PWM (1 µg/ml) mitogens in Roswell Park Memorial Institute (RPMI) 1640 media, and the production of interferon (IFN)-γ and interleukin-4 (IL-4) in the supernatants was measured by enzyme-linked immunosorbent assay (ELISA)...
February 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28293396/the-mir-383-ldha-axis-regulates-cell-proliferation-invasion-and-glycolysis-in-hepatocellular-cancer
#20
Zhixiong Fang, Langqiu He, Hui Jia, Qiusheng Huang, Dan Chen, Zhiwei Zhang
OBJECTIVES: To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). MATERIALS AND METHODS: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate production assay to explore the function of miR-383 in cell proliferation, invasion and glycolysis in HCC cell lines...
February 2017: Iranian Journal of Basic Medical Sciences
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