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Iranian Journal of Basic Medical Sciences

Mushtaq Ahmed, Aden Razaq, Abdul Razaq, Nadia Mushtaq, Rahmat Ali Khan
Objectives: The objective of this study was to evaluate pharmacological effect of Calligonum polygonoides against Krait snake's venom acetylcholinesterase (AChE) and to extent it for the treatment of Alzheimer's disease. Materials and Methods: Acetylcholinesterase activity was measured using Ellman method with some modification. The kinetic studies of methanolic extract of C. polygonoides against krait ( Bungarus Sindanus ) snake venom AChE was measured with the help of the Lineweaver Burk double reciprocal plot...
August 2018: Iranian Journal of Basic Medical Sciences
Changlai Zhu, Yang Liu, Zongyu Guan, Yi Zhou, Fang Liu, Tianyi Zhang
Objectives: The aim of this study is to explore the potential role of hypoxia/reoxygenation in necroptosis in cultured rat renal tubular epithelial cell line NRK-52E, and further to investigate its possible mechanisms. Materials and Methods: Cells were cultured under different hypoxia-reoxygenation conditions in vitro . MTT assay was used to measure the cell proliferation of cells that were exposed to hypoxia-reoxygenation conditions at different time points. Receptor-interacting protein 1,3 (RIP1 and RIP3) and NF-κB were detected by Western-blot analysis...
August 2018: Iranian Journal of Basic Medical Sciences
Hoda Ranjbar, Iraj Aghaei, Mahmood Moosazadeh, Mohammad Shabani
Objectives: Stress alters sensory and cognitive function in humans and animals. Angiotensin (AT) receptors have demonstrated well-established interactions in sets of physiological phenomena. AT1 receptors can play a part in stress-induced activation of hypothalamic-pituitary-adrenal (HPA) axis; besides angiotensinergic neurotransmission plays a pivotal role in stress-evoked physiological responses. AT1 receptors are also involved in nociception and memory. The objective of the current study was to evaluate the effects of losartan as an AT1R antagonist in locomotor activity, nociception and memory impairments induced by sub-chronic swim stress...
August 2018: Iranian Journal of Basic Medical Sciences
Te Liu, Yuzhuo Wang, Modi Yang, Pu Shao, Lian Duan, Meng Li, Mingji Zhu, Jie Yang, Jinlan Jiang
Objectives: Nowadays, Di-(2-ethylhexyl) phthalate (DEHP) is widely used in different kinds of commercial products as a plasticizer. Previous studies have revealed that exposures to DEHP could be associated with precocious puberty in teenagers, but the exact mechanism is yet to be known. Materials and Methods: In this study, 48 prepubertal Wistar female rats were randomly apportioned into 4 groups and orally treated with 0, 250, 500, and 1000 mg/kg/d DEHP from postnatal day 21 up to 4 weeks...
August 2018: Iranian Journal of Basic Medical Sciences
Mojtaba Karimipour, Morteza Hassanzadeh, Masoumeh Zirak Javanmard, Gholamhossein Farjah
Objectives: Skin flap necrosis is the most common postoperative side effect in reconstructive surgeries. Glutamine (GLN) has been shown to accelerate wound healing process. The purpose of this study was to evaluate the effects of GLN either in free form or in the dipeptide form along with L- alanyl (Ala-GLN) on random skin flaps survival in rats. Materials and Methods: Dorsal skin flaps with caudal bases (8 ×2 cm) were established in 24 adult male Wistar rats. Then, the animals were randomly assigned into 3 groups (n=8)...
August 2018: Iranian Journal of Basic Medical Sciences
Gui-Dong Shi, Xin Cheng, Xian-Hu Zhou, Bao-You Fan, Yi-Ming Ren, Wei Lin, Xiao-Lei Zhang, Shen Liu, Yan Hao, Zhi-Jian Wei, Shi-Qing Feng
Objectives: Schwann cells (SCs) have a wide range of applications as seed cells in the treatment of nerve injury during transplantation. However, there has been no report yet on kinds of proteomics changes that occur in Schwann cells before and after peripheral nerve injury. Materials and Methods: Activated Schwann cells (ASCs) and normal Schwann cells (NSCs) were obtained from adult Wistar rat sciatic nerves. After immunofluorescence identification, we identified differentially expressed proteins in the ASCs and NSCs using isobaric tags for relative and absolute quantitation (iTRAQ) combined with high-resolution Orbitrap liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS)...
August 2018: Iranian Journal of Basic Medical Sciences
Shahrzad Havakhah, Mojtaba Sankian, Gholam Hosein Kazemzadeh, Keyvan Sadri, Hamid Reza Bidkhori, Hojjat Naderi-Meshkin, Alireza Ebrahimzadeh Bideskan, Saeed Niazmand, Ahmad Reza Bahrami, Abolfazl Khajavi Rad
Objectives: Renal ischemia-reperfusion injury (IRI) as a severe condition of acute kidney injury (AKI) is the most common clinical problem with high mortality rates of 35-60% deaths in hospital. Mesenchymal stem cells (MSC) due to unique regenerative characteristics are ideal candidates for the treatment of the ischemic injuries. This work is focused on the administration of MSC to IRI-induced AKI Wistar rats and evaluating their significance in AKI treatment. Material and Methods: Animals underwent surgical procedure and AKI was induced by 40 min bilateral renal pedicle clamping...
August 2018: Iranian Journal of Basic Medical Sciences
Li Wang, Kai Gao, Dan Wang
Objectives: Exercise training is a well-known accelerator for the treatment of chronic heart failure (CHF). The current study aimed to investigate the restorative effects of aerobic interval training (AIT) intervention on myocardial energy metabolism in CHF rats. Materials and Methods: Post-myocardial infarction (MI) heart failure animal model was established. The Sprague-Dawley rats were randomly divided into sham operation group (Sham group), CHF model group, and CHF exercise group (Exercise-CHF group)...
August 2018: Iranian Journal of Basic Medical Sciences
Shirin Farjadian, Mozhgan Moghtaderi, Bent-Alhoda Hoseini-Pouya, Azin Ebrahimpour, Mahboubeh Nasiri
Objectives: Asthma, the most frequent chronic respiratory disease, results from a complex interaction between multiple genes and environmental factors. To date, more than 100 candidate genes and single nucleotide polymorphisms (SNPs) have been reported to be associated with asthma. One of the discovered genes related to asthma is ADAM33 . However, the relationship between ADAM33 gene polymorphisms and asthma is controversial. The aim of this study was to investigate the association between four ADAM33 gene SNPs and susceptibility to asthma in patients from southwestern Iran...
August 2018: Iranian Journal of Basic Medical Sciences
Baohua Su, Deng Li, Jie Xu, Yingbin Zhang, Zhiqing Cai, Max Daniel Kauther, Ruofan Ma
Objectives: The study aimed to uncover the underlying mechanism linking wear particles to osteoclast differentiation, and we explored the effect of titanium particles of different sizes on CD147 expression and autophagy in macrophages. Materials and Methods: Effects of titanium particles on CD147 and RANKL mRNA were detected by QPCR; protein level of CD147 and Beclin-1 were detected by Western blot; soluble RANKL were detected by ELISA. To determine the effect of CD147 and autophagy, KG-1a cells were transfected with siRNA-CD147 or treated with autophagy inhibitor CQ (chloroquine), and then co-cultured with different sizes of titanium particles...
August 2018: Iranian Journal of Basic Medical Sciences
Malihe Ardakanizade
Objectives: Long-term, irregular endurance exercise may result in disturbance to the angiogenesis of heart muscles and blood supply. The aim of the present study is to evaluate the effects of mid- and long-term endurance exercise on the process of angiogenesis. Materials and Methods: Eighteen male Wister rats of 220±10 g, were randomly assigned to three groups of 6 rats including: Control, Mid, and Long Group. After the training sessions, the rats were weighed and sacrificed...
August 2018: Iranian Journal of Basic Medical Sciences
Aylin Moayedi, Jamileh Nowroozi, Abbas Akhavan Sepahy
Objectives: Pyocyanin, a blue-green pigment produced by Pseudomonas aeruginosa , interferes with host redox cycles, which can lead to generation of reactive oxygen species and progressive cellular oxidative damage. The aim of this study was to assess the influence of pyocyanin on human pancreatic cancer cell line. Materials and Methods: Polymerase Chain Reaction (PCR) was applied to confirm the existence of a specific pyocyanin producing gene (phzM). The pigment was then characterized by UV-visible, FT-IR, and HPLC analysis...
August 2018: Iranian Journal of Basic Medical Sciences
Halil Yılmaz, Tolga Ertekin, Emre Atay, Mehtap Nisari, Hatice Susar Güler, Özge Al, Ahmet Payas, Seher Yılmaz
Objectives: This study investigated the possible effects of low (3 mg/kg) and high (6 mg/kg) doses of nicotine on the skeletal development of rat fetuses by the double staining method and the protective role of melatonin (10 mg/kg) against these effects. Materials and Methods: Eighteen adult female Wistar-Albino rats were divided into six groups (n=3, each) as control, low-dose nicotine, high-dose nicotine, low-dose nicotine+melatonin, high-dose nicotine + melatonin and melatonin...
August 2018: Iranian Journal of Basic Medical Sciences
Maryam Azarfarin, Fereshteh Farajdokht, Shirin Babri, Farzad Salehpour, Milad Taghizadeh, Gisou Mohaddes
Objectives: Chronic stress has been linked to the pathophysiology of mood disorders including anxiety and depression. In this study, we aimed to investigate the effect of troxerutin (TRX), as a flavonol, on stress-induced anxiety and depression. Materials and Methods: 56 animals were randomly divided into seven groups (n=8 per group) as follows: control, saline, TRX 50, TRX 150, TRX 300, Diazepam, and Imipramine. Chronic mild stress (CMS) was induced by restraining animals in Plexiglas cylinders for 1 hr each day for 25 consecutive days...
August 2018: Iranian Journal of Basic Medical Sciences
Abed Zahedi Bialvaei, Vajihe Sheikhalizadeh, Ali Mojtahedi, Gholamreza Irajian
Objectives: Listeria monocytogenes is a foodborne pathogenic bacteria causing the infection listeriosis, which possibly affects all people, particularly immunocompromised persons and pregnant women. This microorganism can be found in several processed foods, dairy products, raw milk, meat and fish products, seafoods, eggs, fruits, and vegetables. This review discusses about the epidemiological significance, incidence, contamination routes of L. monocytogenes in different products and current data about listeriosis in the Iran...
August 2018: Iranian Journal of Basic Medical Sciences
Zulfiqar Ali Mirani, Aiman Fatima, Shaista Urooj, Mubashir Aziz, Mohammad Naseem Khan, Tanveer Abbas
Objectives: This study was designed to determine the relationship of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli isolates in multispecies biofilms and their individual phenotypic characters in biofilm consortia. Materials and Methods: The subject isolates were recovered from different food samples and identified on the basis of growth on differential and selective media. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study biofilms phenotypes...
July 2018: Iranian Journal of Basic Medical Sciences
Ali Torkashvand, Fariborz Bahrami, Minoo Adib, Soheila Ajdary
Objectives: After decades of containment, pertussis disease, caused by Bordetella pertussis seems to be re-emerging and still remains a major cause of reported vaccine-preventable deaths worldwide. The current licensed whole-cell vaccines display reactogenicity while acellular vaccines are expensive and do not induce Th1-type immune responses that are required for optimum protection against the disease. Thus, there is an urgent need to develop new vaccines and the recombinant technology seems to be the method of choice for this purpose...
July 2018: Iranian Journal of Basic Medical Sciences
Wahyu Widowati, Ervi Afifah, Tjandrawati Mozef, Ferry Sandra, Rizal Rizal, Annisa Amalia, Yukko Arinta, Indra Bachtiar, Harry Murti
Objectives: This study aimed to determine the collagen type II (COL2) and SOX9 expression in interleukin growth factor (IGF-1)-induced Wharton's Jelly mesenchymal stem cells (WJMSCs) and the level of chondrogenic markers in co-culture IGF1-WJMSCs and IL1β-CHON002 as osteoarthritis (OA) cells model. Materials and Methods: WJMSCs were induced with IGF1 (75, 150, and 300 ng/ml) to enhance their chondrogenesis capability. The gene expression of SOX9 and COL2 was evaluated with quantitative RT-PCR...
July 2018: Iranian Journal of Basic Medical Sciences
Hamid Sadeghian, Seyed Mohammad Seyedi, Zeinab Jafari
Objectives: 15-Lipoxygenases are one of the iron-containing proteins capable of performing peroxidation of unsaturated fatty acids in animals and plants. The critical role of enzymes in the formation of inflammations, sensitivities, and some cancers has been demonstrated in mammals. The importance of enzymes has led to the development of mechanistic studies, product analysis, and synthesis of inhibitors. Materials and Methods: The inhibitory activity of all synthetic compounds against SLO (soybean 15-lipoxygenase: L1; EC 1,13,11,12) was determined using the peroxide formation method...
July 2018: Iranian Journal of Basic Medical Sciences
Hong-Liang Kong, Ai-Jie Hou, Ning-Ning Liu, Bo-Han Chen, Sheng-Nan Dai, Hua-Ting Huang
Objectives: This study intended to investigate the effects of Ginsenoside-Rbl (Gs-Rbl) on fatty acid β-oxidation (FAO) in rat failing heart and to identify potential mechanisms of Gs-Rbl improving heart failure (HF) by FAO pathway dependent on AMP-activated protein kinase (AMPK). Materials and Methods: Rats with chronic HF, induced by adriamycin ( Adr ), were randomly grouped into 7 groups. Gs-Rb1, adenine 9-β-D-arabinofuranoside ( Ara A , specific AMPK inhibitor), and 5'-aminoimidazole-4-carboxamide riboside ( Aicar , specific AMPK activator) were administered to rats with HF, singly and/or combinedly...
July 2018: Iranian Journal of Basic Medical Sciences
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