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Iranian Journal of Basic Medical Sciences

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https://www.readbyqxmd.com/read/29147495/standardized-cannabis-sativa-extract-attenuates-tau-and-stathmin-gene-expression-in-the-melanoma-cell-line
#1
Golnaz Vaseghi, Mohamad Javad Taki, Shaghayegh Haghjooy Javanmard
Objectives: Metastasis is the main cause of death in patients with melanoma. Cannabis-based medicines are effective adjunctive drugs in cancer patients. Tau and Stathmin proteins are the key proteins in cancer metastasis. Here we have investigated the effect of a standardized Cannabis sativa extract on cell migration and Tau and Stathmin gene expression in the melanoma cell line. Materials and Methods: In the treatment group, melanoma (B1617) was treated 48 hr with various concentrations of standardized C...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147494/renoprotective-effect-of-crocin-following-liver-ischemia-reperfusion-injury-in-wistar-rats
#2
Seyyed Ali Mard, Ghaidafeh Akbari, Esrafil Mansouri, Mahdi Parsanahad
Objectives: The objectives of the current study were to evaluate the effects of hepatic ischemia/reperfusion (IR) injury on the activity of antioxidant enzymes, biochemical factors, and histopathological changes in rat kidney, and to investigate the effect of crocin on IR-related changes. Materials and Methods: Thirty-two male Wistar rats were randomly allocated into four groups (n=8). They were sham-operated, IR, crocin pre-treatment, and crocin pretreatment+IR groups...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147493/the-effect-of-the-ovarian-varicose-vein-on-the-dna-methylation-in-the-rat-s-oocyte
#3
Amirhossein Mohammadi, Bagher Minaei Zangi, Mahshid Delfan Azari, Rafieh Alizadeh, Mohammad Salehi, Erfan Daneshi, Mohammad Jafar Rezaei, Mehdi Abbasi
Objectives: We intended to determine whether the ovarian varicose which is one of the common etiologies of the pelvic congestion syndrome, has the ability to interfere with the DNA methylation reprogramming in the oocyte and thereby affect the oocyte quality or not. Materials and Methods: Varicose model was induced according to the Turner's method in the rats. Briefly, a 20-gauge needle was placed on the left renal vein and a thread was tied over both the needle and the renal vein medial to the insertion of the ovarian vein, and then the needle was removed...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147492/alterations-in-adult-hippocampal-neurogenesis-aberrant-protein-s-nitrosylation-and-associated-spatial-memory-loss-in-streptozotocin-induced-diabetes-mellitus-type-2-mice
#4
Aneeqa Noor, Saadia Zahid
Objectives: Epidemiological and biochemical studies conducted over the past two decades have established a strong link between type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD). However, the exact mechanisms through which aberrations in insulin signaling associated with T2DM contribute to cognitive decline are not yet known. Materials and Methods: In an effort to explore possible molecular links between T2DM and AD, the present study investigated the status of neurodegeneration, adult hippocampal neurogenesis, and nitrosative stress induced protein S-nitrosylation in streptozotocin (STZ) induced mice models of T2DM...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147491/augmented-expression-levels-of-lncrnas-eccebpa-and-uca1-in-gastric-cancer-tissues-and-their-clinical-significance
#5
Mojdeh Nasrollahzadeh-Khakiani, Modjtaba Emadi-Baygi, Parvaneh Nikpour
Objectives: As the second cause of cancer death, gastric cancer (GC) is one of the eminent dilemmas all over the world, therefore investigating the molecular mechanisms involved in this cancer is pivotal. Unrestricted proliferation is one of the characteristics of cancerous cells, which is due to deficiency in cell regulatory systems. Long non-coding RNAs (lncRNAs) have emerged as critical regulators of the epigenome. lncRNA extra coding CEBPA (ecCEBPA) is involved in DNA methylation...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147490/hypolipidemic-effects-of-total-flavonoide-extracted-from-the-leaves-of-actinidiakolomikta-in-rats-fed-a-high-fat-diet
#6
Zhenxiang Yu, Huali Xu, Xiaofeng Yu, Dayun Sui, Guangzhu Lin
Objectives: This study was to investigate the antihyperlipidemic and antioxidant effect of total flavonoid extract from Actinidiakolomikta (TFAK) in hyperlipidemia induced by a high-fat diet. Materials and Methods: Male SD rats were randomly divided into 6 groups: normal group, model (hyperlipidemic diet) group, hyperlipedemic diet supplemented with TFAK (50, 100 and 200 mg/kg) and simvastatin (30 mg/kg) groups. The rats were administrated TFAK by oral for 28 days...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147489/the-effect-of-platelet-rich-plasma-on-human-mesenchymal-stem-cell-induced-bone-regeneration-of-canine-alveolar-defects-with-calcium-phosphate-based-scaffolds
#7
Reihaneh Shafieian, Maryam Moghaddam Matin, Amin Rahpeyma, Alireza Fazel, Hamideh Salari Sedigh, Ariane Sadr-Nabavi, Halimeh Hassanzadeh, Alireza Ebrahimzadeh-Bideskan
Objectives: Autologous bone transplantation known as the "gold standard" to reconstruction of osseous defects has known disadvantages. This study was designed to explore the effects of hydroxy-apatite/tricalcium-phosphate (HA/TCP) and platelet-rich plasma (PRP) on the osteogenesis ability of human adipose-derived mesenchymal stem cells (hAdMSCs) in vitro and in vivo. Materials and Methods: hAdMSCs were incubated with HA/TCP granules and/or PRP in vitro and then, cell proliferation and differentiation was assessed by MTT assay, AZR S staining and SEM examination...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147488/the-downregulation-of-atg4b-mediated-by-microrna-34a-34c-5p-suppresses-rapamycin-induced-autophagy
#8
Yaran Wu, Xufang Dai, Zhenhong Ni, Xiaojing Yan, Fengtian He, Jiqin Lian
Objectives: Autophagy-related 4B (ATG4B) plays an important role in the process of autophagy induction. However, the molecular events that govern the expression of ATG4B in this process are not well known. Materials and Methods: Human ATG4B 3'-UTR region (1377 nt) containing miR-34a/miR-34c-5p binding site was amplified by PCR. Luciferase assay was used to assess the activity of reporter genes. Real-time PCR was used to detect the levels of miR-34a and miR-34c-5p...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147487/a-study-on-the-immune-response-induced-by-a-dna-vaccine-encoding-mtb32c-hbha-antigen-of-mycobacterium-tuberculosis
#9
Roghayeh Teimourpour, Hadi Peeridogaheh, Amir Teimourpour, Mohsen Arzanlou, Zahra Meshkat
Objectives: Tuberculosis (TB) has still remained a global health issue. One third of the world's population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses against Mycobacterium tuberculosis. Materials and Methods: A DNA plasmid vaccine expressing Mtb32C-HBHA fusion protein was constructed and its ability in protein expression was examined by RT-PCR and Western blot methods...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147486/a-novel-medium-throughput-biological-assay-system-for-htlv-1-infectivity-and-drug-discovery
#10
Mojtaba Fattahi Abdizadeh, Manoochehr Makvandi, Alireza Samarbafzadeh, Kayhan Azadmanesh
Objectives: Here, a reporter cell line containing two reporter vectors were developed, in order to monitor the Human T-Lymphotropic Virus type1(HTLV-1) infectivity and the cell viability simultaneously. Materials and Methods: The reporter cell line was constructed by stably transfected baby hamster's kidney cell line (BHK-21), with the genomes expressing two different reporters in separate plasmids. The first reporter gene is transactivated by the HTLV-1 tax protein, while the second reporter is continuously expressed when introduced into a mammalian cell...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147485/the-effects-of-anti-fas-ribozyme-on-t-lymphocyte-apoptosis-in-mice-model-with-chronic-obstructive-pulmonary-disease
#11
Song-Ming Zhuo, Si-Cong Li, Yong-Qun Lin, Hai-Bin Yu, Na Li
Objectives: In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD). Materials and Methods: Male 6-week-old C57BL/6 mice were used to establish the COPD model by exposure to cigarette smoke. The COPD mice were sacrificed for spleen dissection and T cell isolation. T cells were randomly divided into four groups (n=10 per group). Group A was used as the control...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147484/berberis-integerrima-ameliorates-insulin-resistance-in-high-fructose-fed-insulin-resistant-rats
#12
Hossein Fallah, Hamed Akbari, Moslem Abolhassani, Abbas Mohammadi, Ahmad Gholamhosseinian
Objectives: This study was aimed to investigate the effect of Berberis integerrima (B. integerrima) extract on insulin sensitivity in high-fructose-fed insulin-resistant rats. Materials and Methods: Experimental rats were randomly divided into two groups: the control group was fed a regular chow diet while other group fed with a high-fructose diet for 8 weeks. After the first six weeks, the animals were treated with B. integerrima extract or pioglitazone for two weeks...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147483/sonophotodynamic-therapy-mediated-by-liposomal-zinc-phthalocyanine-in-a-colon-carcinoma-tumor-model-role-of-irradiating-arrangement
#13
Maryam Bakhshizadeh, Toktam Moshirian, Habibollah Esmaily, Omid Rajabi, Hooriyeh Nassirli, Ameneh Sazgarnia
Objectives: Low penetration depth of light is the main defect of photodynamic therapy (PDT), which could be improved by sonodynamic therapy (SDT). In this study, a combination of PDT and SDT known as sonophotodynamic therapy (SPDT) was investigated using two reverse arrangements in CT26 tumor model. Materials and Methods: The liposomal zinc phthalocyanine was synthesized and characterized. It was then administered to CT26 tumor models as a sensitizer. The animal models were subjected to PDT, SDT, and the combined treatment in different groups...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147482/combined-postconditioning-with-ischemia-and-cyclosporine-a-restore-oxidative-stress-and-histopathological-changes-in-reperfusion-injury-of-diabetic-myocardium
#14
Reza Badalzadeh, Seyed Mahmoud Tabatabaei, Mustafa Mohammadi, Arash Khaki, Dariush Mohammadnezhad
Objectives: Chronic diabetes impedes cardioprotection in reperfusion injury and hence protecting the diabetic heart would have important outcomes. In this study, we evaluated whether combined postconditioning with ischemia and cyclosporine-A can restore oxidative stress and histopathological changes in reperfusion injury of the diabetic myocardium. Materials and Methods: Streptozocin-induced diabetic hearts and nondiabetic controls in eight subgroups (with or without receiving ischemic-postconditioning (IPostC), cyclosporine-A, an inhibitor of mitochondrial permeability transition, or both of them) suffered from 30 min regional ischemia followed by 45 min reperfusion on an isolated-heart Langendorff system...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29147481/is-precocious-puberty-linked-to-hypothalamic-expression-of-arginine-phenylalanine-amide-related-peptide
#15
REVIEW
Yuanyuan He, Wen Sun, Jian Yu
The up-regulation and down-regulation of gonadotropin-releasing hormone (GnRH) in central precocious puberty is not yet known. However, recent advances in neuroendocrinology have shown the controlling role of arginine-phenylalanine RF-amide-related peptides (RFRPs) on GnRH secretion in different phenomenon of reproduction such as estrus cycle and pregnancy, but the exact role of RFRPs in puberty and its related pathologic condition, precocious puberty, is not clear yet. This paper hypothesizes that RFRP is a regulatory peptide of puberty and might prevent the precocious puberty...
October 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29085603/hydroethanolic-extract-of-carthamus-tinctorius-induces-antidepressant-like-effects-modulation-by-dopaminergic-and-serotonergic-systems-in-tail-suspension-test-in-mice
#16
Saeid Abbasi-Maleki, Zahra Mousavi
OBJECTIVES: Studies indicate that major deficiency in the levels of monoaminergic transmitters is a reason for severe depression. On the other hand, it is shown that Carthamus tinctorius L. (CT) may improve neuropsychological injuries by regulation of the monoamine transporter action. Hence, the present study was undertaken to evaluate the involvement of monoaminergic systems in antidepressant-like effect of CT extract in the tail suspension test (TST) in mice. MATERIALS AND METHODS: The mice were intraperitoneally (IP) treated with CT extract (100-400 mg/kg) 1 hr before the TST...
September 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29085602/calycosin-attenuates-dextran-sulfate-sodium-dss-induced-experimental-colitis
#17
Liu Chao, Pengyuan Zheng, Liu Xia, Yu Yong, Gaofeng Lu, Fuai Tang, Zhiguo Zhao
OBJECTIVES: Inflammatory bowel disease (IBD) results from dysregulation of intestinal mucosal immunity. It is an incurable disease that affects millions of people worldwide. Developing new strategies for the treatment of colitis has been a major challenge. Here, we report the effect of calycosin, a plant-derived flavonoid, in successfully managing colitis in murine model. MATERIAL AND METHODS: In vivo model of colitis was induced using 2.5% (w/v) dextran sodium sulfate (DSS, 36,000 to 50,000 Mw)...
September 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29085601/presenting-a-rapid-method-for-detection-of-bacillus-cereus-listeria-monocytogenes-and-campylobacter-jejuni-in-food-samples
#18
Ali Razei, Rahim Sorouri, Seyed Latif Mousavi, Shahram Nazarian, Jafar Amani, Hosien Aghamollaei
OBJECTIVES: Listeria monocytogens, Bacillus cereus and Campylobacter jejuni are three toxin producing bacteria over the world, especially in Iran, and it is essential to find a certain, rapid procedure to identify these microorganisms. In this research, these bacteria were simultaneously detected by multiplex PCR technique in foods. MATERIALS AND METHODS: The primary approval of bacterial strains was performed by biochemical tests. PCR primers were designed based on the nucleotide sequences of the NHEB/NHEC gene of B...
September 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29085600/mechanism-underlying-the-effects-of-doxepin-on-%C3%AE-amyloid-induced-memory-impairment-in-rats
#19
Jimei Bu, Hengbing Zu
OBJECTIVES: In previous studies, researchers observed that doxepin could improve cognitive processes and has protective effects on the central nervous system. Thus, this study was designed to analyze the effects of doxepin on β-amyloid (Aβ)-induced memory impairment and neuronal toxicity in rat and to explore the underlying mechanism. MATERIALS AND METHODS: Rats were treated with Aβ1-42 and doxepin was injected to validate its effects on cognitive function. The Morris water maze test was performed to detect memory function...
September 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29085599/evaluation-of-bax-bcl-2-p21-and-p53-genes-expression-variations-on-cerebellum-of-balb-c-mice-before-and-after-birth-under-mobile-phone-radiation-exposure
#20
Najmeh Ghatei, Ariane Sadr Nabavi, Mohammad Hossein Bahreyni Toosi, Hosein Azimian, Mansour Homayoun, Reza Ghasemnezhad Targhi, Hossein Haghir
OBJECTIVES: The increasing rate of over using cell phones has been considerable in youths and pregnant women. We examined the effect of mobile phones radiation on genes expression variation on cerebellum of BALB/c mice before and after of the birth. MATERIALS AND METHODS: In this study, a mobile phone jammer, which is an instrument to prevent receiving signals between cellular phones and base transceiver stations (two frequencies 900 and 1800 MHz) for exposure was used and twelve pregnant mice (BALB/c) divided into two groups (n=6), first group irradiated in pregnancy period (19th day), the second group did not irradiate in pregnancy period...
September 2017: Iranian Journal of Basic Medical Sciences
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