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Avicenna Journal of Medical Biotechnology

Rouhollah Gazor, Mozhgan Eskandari, Alireza Sharafshah, Mohammad Hadi Bahadori, Mohammad Ghasem Golmohammadi, Parvaneh Keshavarz
Background: Exact mechanisms of fetal harm following vitrification are still unknown. This study was conducted to evaluate the cryopreservation impact on the expression of Epidermal Growth Factor Receptor (EGFR) gene in mouse 2-cell and blastocysts. Methods: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-cells and blastocysts after 44-46 and 88-89 hr , respectively. These embryos were divided into two case and control groups. The fresh case group was cryopreserved using cryotop and warmed after 4 mounts...
April 2018: Avicenna Journal of Medical Biotechnology
Hesam Mokhtari, Gilda Eslami, Hengameh Zandi, Amin Dehghan-Banadkouki, Mahmood Vakili
Background: Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen that could be resistant to many antimicrobial agents. Resistance genes can be carried among gram-negative bacteria by integrons. Enzymatic inactivation is the most important mechanism of resistance to aminoglycosides. In this study, the frequencies of two important resistance gene aac(6')-II a and ant(2″)-I, and genes coding integrase I and II, in K. pneumoniae isolates resistant to aminoglycosides were evaluated...
April 2018: Avicenna Journal of Medical Biotechnology
Tahereh Sadeghian-Rizi, Fereshteh Alsahebfosoul, Mohammad Kazemi, Hossein Khanahmad, Ali Jahanian-Najafabadi
Background: Multiple Sclerosis (MS) is the most common cause of neurologic disability in young adults. Recently, the AIRE gene was identified as a genetic risk factor for several autoimmune diseases in genome wide association studies. The aim of this study was to further investigate the possible role of the AIRE gene in susceptibility to MS in Iranian population. Methods: A total of 112 MS patients and 94 ethnically matched controls were included in the study. The Single-Nucleotide Polymorphism (SNP) (rs1800520, C>G) with a global MAF=0...
April 2018: Avicenna Journal of Medical Biotechnology
Mona Hedayat, Mohammad Jafar Mahmoudi, Mohammad Taghvaei, Ebrahim Nematipour, Elham Farhadi, Nilufar Esfahanian, Maryam Mahmoudi, Maryam Sadr, Keramat Nourijelyani, Ali Akbar Amirzargar, Nima Rezaei
Background: Proinflammatory cytokines have been known to be elevated in patients with Chronic Heart Failure (CHF). Given the importance of proinflammatory cytokines in the context of the failing heart, the prevalence of Tumor Necrosis Factor-α (TNF-α), Interleukin (IL)-6 polymorphisms in patients with CHF was studied due to ischemic heart disease. Methods: Forty three patients with ischemic heart failure were enrolled in this study and compared with 140 healthy individuals...
April 2018: Avicenna Journal of Medical Biotechnology
Sayed Mostafa Hosseini, Bahram Mohammad Soltani, Mahmoud Tavallaei, Seyed Javad Mowla, Elham Tafsiri, Abouzar Bagheri, Hamid Reza Khorram Khorshid
Background: The cyclin E2 (CYCE2) is an important regulator in the progression and development of NSCLC, and its ectopic expression promoted the proliferation, invasion, and migration in several tumors, including Non-Small Cell Lung Cancer (NSCLC). However, the upregulation of CYCE2 in NSCLC cells suggested that it has a key role in tumorigenicity. In addition, the RAS family proteins as oncoproteins were activated in many major tumor types and its suitability as the therapeutic target in NSCLC was proposed...
April 2018: Avicenna Journal of Medical Biotechnology
Babak Qasemi-Panahi, Mansoureh Movahedin, Gholamali Moghaddam, Parviz Tajik, Mortaza Koruji, Javad Ashrafi-Helan, Seyed Abbas Rafat
Background: Sheep industry has taken steps toward transforming itself into a more efficient and competitive field. There are many varieties of sheep breeds in the world that each of them serves a useful purpose in the economies of different civilizations. Ghezel sheep is one of the Iranian important breeds that are raised for meat, milk and wool. Field of spermatogonial cell technologies provides tools for genetic improvement of sheep herd and multiple opportunities for research. Spermatogonial cells are the only stem cells capable of transmitting genetic information to future generations...
April 2018: Avicenna Journal of Medical Biotechnology
Afsaneh Arefi Oskouie, Reyhaneh Farrokhi Yekta, Mostafa Rezaei Tavirani, Masoud Soheili Kashani, Fatemeh Goshadrou
Background: Alzheimer's Disease (AD) is the most prevalent cause of memory impairment in the elderly population, but the diagnosis and treatment of the disease is still challenging. Lavender aqueous extract has recently been shown to have the potential in clearing Amyloid-beta plaques from AD rat hippocampus. To elucidate the therapeutic mechanisms of lavender, serum metabolic fingerprint of Aβ-induced rat Alzheimer's models was investigated through nuclear magnetic resonance spectrometry...
April 2018: Avicenna Journal of Medical Biotechnology
Maryam Khaleghizadeh, Mohammad Mahdi Forghanifard, Abolfazl Rad, Moein Farshchian, Zahra Hejazi, Mehran Gholamin, Bahram Memar, Mohammad Reza Abbaszadegan
Background: Cancer/Testis Antigens (CTAs) are a sub-group of tumor-associated antigens which are expressed normally in germ line cells and trophoblast, and aberrantly in a variety of malignancies. One of the most important CTAs is Developmental Pluripotency Associated-2(DPPA2) with unknown biological function. Considering the importance of DPPA2 in developmental events and cancer, preparing a suitable platform to analyze DPPA2 roles in the cells seems to be necessary. Methods: In this study, the coding sequence of DPPA2 gene was amplified and cloned into the retroviral expression vector to produce recombinant retrovirus...
April 2018: Avicenna Journal of Medical Biotechnology
Nazem Ghasemi
Background: Multiple Sclerosis (MS) has been explained as an autoimmune mediated disorder in central nerve system. Since conventional therapies for MS are not able to stop or reverse the destruction of nerve tissue, stem cell-based therapy has been proposed for the treatment of MS. Astaxanthin (AST) is a red fat-soluble xanthophyll with neuroprotection activity. The aim of this study was evaluation of pre-inducer function of AST on differentiation of human Adipose-Derived Stem Cells (hADSCs) into oligodendrocyte precursor cells...
April 2018: Avicenna Journal of Medical Biotechnology
Sina Baharaghdam, Mehdi Yousefi, Aliakbar Movasaghpour, Saeed Solali, Mehdi Talebi, Milad Ahani-Nahayati, Hamid Lotfimehr, Karim Shamsasanjan
Background: One of the most significant problems in the treatment of leukemia is the expansion of resistance to chemotherapeutic agents. Therefore, assessing the drug resistance and especially the drug resistance genes of leukemic cells is important in any treatment. The impact of Mesenchymal Stem Cells (MSCs) and hypoxic condition have been observed in the biological performance of majority of leukemic cells. Methods: MOLT-4 cells were co-cultured with MSCs in the hypoxic condition induced by Cobalt Chloride (CoCl2 ) for 6 and 24 hr ...
April 2018: Avicenna Journal of Medical Biotechnology
Mahmood Jeddi-Tehrani
No abstract text is available yet for this article.
April 2018: Avicenna Journal of Medical Biotechnology
Azam Moosavi, Ali M Ardekani
[This retracts the article on p. 196 in vol. 9, PMID: 29090069.].
April 2018: Avicenna Journal of Medical Biotechnology
Shamsolmolouk Najafi, Mahsa Mohammadzadeh, Amirabbas Zahedi, Mansour Heidari, Nima Rezaei
Background: Recurrent Aphthous Stomatitis (RAS) is one of the most common diseases of the oral cavity all over the world (5-66%). RAS has a multifactorial etiology, while psychological factors such as stress and anger play a role in its manifestation. The serotonergic mechanisms particularly the serotonin-transporter gene ( 5-HTT ) may affect the risk of psychological alterations and stress response. The aim of the present study was to evaluate the polymorphism of the promoter region of 5-HTT ( 5-HTTLPR ) in the patients with RAS, compared to that in the control subjects...
January 2018: Avicenna Journal of Medical Biotechnology
Marjan Oraei, Seyed Hadi Razavi, Faramarz Khodaiyan
Background: Riboflavin (vitamin B2 ) is an essential component of the basic metabolism, and an important nutritional and growth factor in humans, animals, plants and micro-organisms. It has been widely used in the fields of pharmaceuticals, feed and food additives. The industrial production of riboflavin mostly relies on the microbial fermentation. Designing an appropriate fermentation medium is of crucial importance to improve the riboflavin production. Methods: In this study, sequential methodology combining a screening test of minerals by Plackett-Burman (PB) and an optimization test by Central Composite Design (CCD) was applied to enhance riboflavin production by Bacillus subtilis ATCC 6051 in shake flasks...
January 2018: Avicenna Journal of Medical Biotechnology
Abolfazl Shirazi, Arefeh Golestanfar, Masomeh Bashiri, Ebrahim Ahmadi, Naser Shams-Esfandabadi
Background: Failure of Male Pronucleus (MPN) formation is a major concern in the success of Intracytoplasmic Sperm Injection (ICSI) in some species. Despite the conducted unsuccessful efforts to improve ICSI efficiency in ovine, the present study was aimed to improve MPN formation and embryo development in ovine ICSI procedure through accompaniment of sperm pretreatment with co-injection of some compounds. Methods: In experiment 1, sperm were treated with either 2-mercaptoethanol (2ME), glutathione (GSH), or DTT (dithiothreitol) in combination with Heparin (Hep)...
January 2018: Avicenna Journal of Medical Biotechnology
Sobhan Faezi, Iraj Nikokar, Ali Elmi, Yusuf Ghasemi, Mojtaba Farahbakhsh, Alireza Salimi Chirani, Mehdi Mahdavi
Background: Type 4 pili (T4P) is an important virulence factor of Pseudomonas aeruginosa (P. aeruginosa) . T4P pass the outer membrane through a large oligomeric channel made of a single PilQ protein that is most highly conserved at their C-termini. To develop a functional vaccine that can be used in clinical application, the secretin domain of the PilQ (PilQ380-706 ) was produced as a recombinant protein. Methods: A 981 bp fragment of C-terminal of the pilQ secretin ( pilQ 1138-2118 ) from was designed into the prokaryotic expression vector pET28a...
January 2018: Avicenna Journal of Medical Biotechnology
Hamid Kabiri Rad, Mahta Mazaheri, Ali Dehghani Firozabadi
Background: MicroRNAs (miRNAs) are implicated in various biological processes including anticoagulation. However, the modulation of miRNA by pharmacological intervention such as warfarin treatment in patients receiving warfarin has not been disclosed yet. The aim of this study work was to assess the effect of warfarin drug on expression level of mir-133a-3p in patients with mechanical heart valve replacement. Methods: In this research, the expression level of miRNA-133a-3p was analyzed in Peripheral Blood Mononuclear Cells (PBMCs) from mechanical valve replacement patients who had received warfarin for at least 3 months continuously...
January 2018: Avicenna Journal of Medical Biotechnology
Sara Moradi, Alireza Fazlali, Hamid Hamedi
Background: Hydro-distillation (HD) method is a traditional technique which is used in most industrial companies. Microwave-assisted Hydro-distillation (MAHD) is an advanced HD technique utilizing a microwave oven in the extraction process. Methods: In this research, MAHD of essential oils from the aerial parts (leaves) of rosemary ( Rosmarinus officinalis L. ) was studied and the results were compared with those of the conventional HD in terms of extraction time, extraction efficiency, chemical composition, quality of the essential oils and cost of the operation...
January 2018: Avicenna Journal of Medical Biotechnology
Sorina Hirbod, Shohreh Nafisi, Howard I Maibach
Background: β-Cyclodextrin functionalized hyper-branched polyglycerol (HBCD: β-CD-g-PG), a biocompatible polymer, has recently been proposed for delivery of poorly water soluble compounds. Methods: The present study examines the interaction of HBCD with RNA, utilizing a constant concentration of RNA and different HBCD/RNA ratios of 1/16 to 1/1, at physiological condition in an aqueous solution. Circular Dichroism (CD), UV-visible, FTIR spectroscopic methods, zeta potential and Dynamic Light Scattering (DLS) were used to analyze the particle formation, particle charge, particle size, aggregation, RNA conformation, binding constant and mode, and the effect of polymer complexation on RNA stability...
January 2018: Avicenna Journal of Medical Biotechnology
Bahram Baghban Kohnehrouz, Afsaneh Talischian, Alireza Dehnad, Shahnoush Nayeri
Background: Avimers are originally types of artificial proteins with multiple binding sites for specific binding to certain antigens. Various radioisotopes and nanoparticles link these molecules, which are widely used in early detection in tissue imaging, treatment and study on carcinogenesis. Among these, c-Met antagonist avimer (C426 avimer), with ability to bind the c-Met receptor of tyrosine kinase (RTK) is an attractive candidate for targeted cancer therapy. In this study, a novel traceable C426 avimer gene was designed and introduced by adding the 12nt tracer binding site encoded four specific amino acid residues at the C-terminal region of C426 avimer coding sequence...
January 2018: Avicenna Journal of Medical Biotechnology
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