Read by QxMD icon Read

Avicenna Journal of Medical Biotechnology

Sebahat Turgut, Senay Topsakal, Melek Tunç Ata, Duygu Herek, Fulya Akın, Şeyma Özkan, Günfer Turgut
BACKGROUND: Acromegaly is associated with increased morbidity and mortality related to cardiovascular diseases. Leptin (LEP) and Leptin Receptor (LEPR) gene polymorphisms can increase cardiovascular risks. The aim of this study was to investigate association between the frequencies of LEP and LEPR gene polymorphisms and subclinical atherosclerosis in acromegalic patients. METHODS: Forty-four acromegalic patients and 30 controls were admitted to study. The polymorphisms were identified by using polymerase chain reaction from peripheral blood samples...
July 2016: Avicenna Journal of Medical Biotechnology
Mohammad Mehdi Naderi, Sara Borjian Boroujeni, Ali Sarvari, Banafsheh Heidari, Mohammad Mehdi Akhondi, Amir-Hassan Zarnani, Abolfazl Shirazi
BACKGROUND: This study was aimed to assess the effects of angiotensin II (Ang II) supplementation to the In Vitro Maturation (IVM) and In Vitro Culture (IVC) media of vitrified-warmed ovine oocytes on their developmental competence and expression of Na(+)/K(+)/ATPase in resulting embryos. METHODS: The slaughterhouse-derived immature oocytes (n=1069) were randomly distributed into four experimental groups: groups I and II) IVM/IVF and IVC of fresh and vitrified oocytes without angiotensin supplementation (Control-Fresh and Control-Vit groups, respectively); group III) IVM of vitrified oocytes in the presence of Ang II followed by IVF/IVC (Vit-IVM group); and group IV) IVM/IVF of vitrified oocytes followed by IVC wherein the embryos were exposed to Ang II on day 4 of IVC (Vit-D4 group)...
July 2016: Avicenna Journal of Medical Biotechnology
Vahid Akbarinejad, Parviz Tajik, Mansoureh Movahedin, Reza Youssefi
BACKGROUND: Niche cells, regulating Spermatogonial Stem Cells (SSCs) fate are believed to have a reciprocal communication with SSCs. The present study was conducted to evaluate the effect of SSC elimination on the gene expression of Glial cell line-Derived Neurotrophic Factor (GDNF), Fibroblast Growth Factor 2 (FGF2) and Kit Ligand (KITLG), which are the main growth factors regulating SSCs development and secreted by niche cells, primarily Sertoli cells. METHODS: Following isolation, bovine testicular cells were cultured for 12 days on extracellular matrix-coated plates...
July 2016: Avicenna Journal of Medical Biotechnology
Ali Akbar Moosavi-Movahedi, Naghmeh Sattarahmady, Esmaeil Sharifi, Hossein Heli
BACKGROUND: Protein aggregation is one of the important, common and troubling problems in biotechnology, pharmaceutical industries and amyloid-re-lated disorders. METHODS: In the present study, the inhibitory effects of some carbohydrates (alginate, β-cyclodextrin and trehalose) on the formation of nano-globular aggregates from normal (HSA) and glycated (GHSA) human serum albumin were studied; when the formation of aggregates was induced by the simultaneous heating and addition of dithiotheritol...
July 2016: Avicenna Journal of Medical Biotechnology
Parvaneh Daneshmand, Kioomars Saliminejad, Marzieh Dehghan Shasaltaneh, Koorosh Kamali, Gholam Hossein Riazi, Reza Nazari, Pedram Azimzadeh, Hamid Reza Khorram Khorshid
BACKGROUND: Sporadic Alzheimer's Disease (SAD) is caused by genetic risk factors, aging and oxidative stresses. The herbal extract of Rosa canina (R. canina), Tanacetum vulgare (T. vulgare) and Urtica dioica (U. dioica) has a beneficial role in aging, as an anti-inflammatory and anti-oxidative agent. In this study, the neuroprotective effects of this herbal extract in the rat model of SAD was investigated. METHODS: The rats were divided into control, sham, model, herbal extract -treated and ethanol-treated groups...
July 2016: Avicenna Journal of Medical Biotechnology
Javad Baharara, Elaheh Amini, Najme Nikdel, Farzaneh Salek-Abdollahi
BACKGROUND: Malignant melanoma is a highly aggressive malignant melanocytic neoplasm which resists against the most conventional therapies. Sea cucumber as one of marine organisms contains bioactive compounds such as polysaccharide, terpenoid and other metabolites which have anti-cancer, anti-tumor, anti-inflammatory and antioxidant properties. The present study was designed to investigate the anticancer potential of saponin extracted from sea cucumber Holothuria leucospilata alone and in combination with dacarbazine on B16F10 melanoma cell line...
July 2016: Avicenna Journal of Medical Biotechnology
Faezeh Almasi, Seyed Latif Mousavi Gargari, Fatemeh Bitaraf, Samaneh Rasoulinejad
BACKGROUND: Nowadays, highly specific aptamers generated by cell SELEX technology (systematic evolution of ligands by exponential enrichment) are being applied for early detection of cancer cells. Prostate Specific Membrane Antigen (PSMA), over expressed in prostate cancer, is a highly specific marker and therefore can be used for diagnosis of the prostate cancer cells. The aim of the present study was to select single-stranded DNA aptamers against LNCap cells highly expressing PSMA, using cell-SELEX method which can be used as a diagnostic tool for the detection of prostate cancer cells...
July 2016: Avicenna Journal of Medical Biotechnology
Somaieh Kazemnejad
No abstract text is available yet for this article.
July 2016: Avicenna Journal of Medical Biotechnology
Milad Shahini Shams Abadi, Seyed Davar Siadat, Farzam Vaziri, Mehdi Davari, Abolfazl Fateh, Shahin Pourazar, Farid Abdolrahimi, Morteza Ghazanfari
BACKGROUND: The pathogenesis of nontypeable Haemophilus influenzae (NTHi) begins with adhesion to the rhinopharyngeal mucosa. Almost 38-80% of NTHi clinical isolates produce proteins that belong to the High Molecular Weight (HMW) family of adhesins, which are believed to facilitate colonization. METHODS: In the present study, the prevalence of hmwA, which encodes the HMW adhesin, was determined for a collection of 32 NTHi isolates. Restriction Fragment Length Polymorphism (RFLP) was performed to advance our understanding of hmwA binding sequence diversity...
April 2016: Avicenna Journal of Medical Biotechnology
Hossein Danafar, Mehrdad Hamidi
BACKGROUND: A simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. METHODS: The mobile phase composed of acetonitrile-water 60:40 (v/v) and 0.2% formic acid 20 μl of sample was chromatographically analyzed using a repacked ZORBAX-XDB-ODS C18 column (2.1 mm×30 mm, 3.5 μ). Detection of analytes was achieved by tandem mass spectrometry with Electrospray Ionization (ESI) interface in positive ion mode operated under the multiple-reaction monitoring mode (m/z 230...
April 2016: Avicenna Journal of Medical Biotechnology
Maryam Eini, Abbas Behzad-Behbahani, Mohammad Ali Takhshid, Amin Ramezani, Gholam Reza Rafiei Dehbidi, Mohammad Ali Okhovat, Ali Farhadi, Parniyan Alavi
BACKGROUND: DNA isolation procedure can significantly influence the quantification of DNA by real time PCR specially when cell free DNA (cfDNA) is the subject. To assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfDNA, development of appropriate External DNA Control (EDC) is challenging. Using non-human chimeric nucleotide sequences, an EDC was developed for standardization of qPCR for monitoring stability of cfDNA concentration in blood samples over time...
April 2016: Avicenna Journal of Medical Biotechnology
Javad Baharara, Tayebe Ramezani, Adeleh Divsalar, Marzieh Mousavi, Arefeh Seyedarabi
BACKGROUND: Gold Nanoparticles (GNPs) are used in imaging and molecular diagnostic applications. As the development of a novel approach in the green synthesis of metal nanoparticles is of great importance and a necessity, a simple and safe method for the synthesis of GNPs using plant extracts of Zataria multiflora leaves was applied in this study and the results on GNPs' anticancer activity against HeLa cells were reported. METHODS: The GNPs were characterized by UV-visible spectroscopy, FTIR, TEM, DLS and Zeta-potential measurements...
April 2016: Avicenna Journal of Medical Biotechnology
Raheleh Assaei, Pooneh Mokarram, Sanaz Dastghaib, Sara Darbandi, Mahsa Darbandi, Fatemeh Zal, Masoumeh Akmali, Gholam Hossein Ranjbar Omrani
BACKGROUND: Traditional medicines with anti-diabetic effects are considered suitable supplements to treat diabetes. Among medicinal herbs, Stevia Rebaudiana Bertoni is famous for its sweet taste and beneficial effect in regulation of glucose. However, little is known about the exact mechanism of stevia in pancreatic tissue. Therefore, this study investigated the possible effects of stevia on pancreas in managing hyperglycemia seen in streptozotocin-induced Sprague-Dawley rats. METHODS: Sprague-Dawley rats were divided into four groups including normoglycemic, diabetic and two more diabetic groups in which, one was treated with aquatic extract of stevia (400 mg/kg) and the other with pioglitazone (10 mg/kg) for the period of 28 days...
April 2016: Avicenna Journal of Medical Biotechnology
Neda Mousavi Niri, Arash Memarnejadian, Jamshid Hadjati, Mohammad Reza Aghasadeghi, Mehdi Shokri, Yones Pilehvar-Soltanahmadi, Abolfazl Akbarzadeh, Nosratollah Zarghami
BACKGROUND: It seems that the success of vaccination for cancer immunotherapy such as Dendritic Cell (DC) based cancer vaccine is hindered through a powerful network of immune system suppressive elements in which regulatory T cell is the common factor. Foxp3 transcription factor is the most specific marker of regulatory T cells. In different studies, targeting an immune response against regulatory cells expressing Foxp3 and their removal have been assessed. As these previous studies could not efficiently conquer the suppressive effect of regulatory cells by their partial elimination, an attempt was made to search for constructing more effective vaccines against regulatory T cells by which to improve the effect of combined means of immunotherapy in cancer...
April 2016: Avicenna Journal of Medical Biotechnology
Katayoun Pourvali, Mehrnaz Abbasi, Azadeh Mottaghi
Diabetes Mellitus (DM) is a chronic heterogeneous disorder and oxidative stress is a key participant in the development and progression of it and its complications. Anti-oxidant status can affect vulnerability to oxidative damage, onset and progression of diabetes and diabetes complications. Superoxide dismutase 2 (SOD2) is one of the major antioxidant defense systems against free radicals. SOD2 is encoded by the nuclear SOD2 gene located on the human chromosome 6q25 and the Ala16Val polymorphism has been identified in exon 2 of the human SOD2 gene...
April 2016: Avicenna Journal of Medical Biotechnology
Shahin Akhondzadeh
No abstract text is available yet for this article.
April 2016: Avicenna Journal of Medical Biotechnology
Alaa Abdel-Kadhim Jawad, Alaa H Al-Charrakh
BACKGROUND: The use of selective and differential plating media is a simple method for the isolation of Salmonella spp. Recently, there has been a general move toward molecular methods of Salmonella detection and typing. METHODS: A total of 1200 different specimens collected from human and animal sources were involved in his study. 600 stool specimens from patients suffering from diarrhea and 600 specimens from gall bladder (bile) of cattle from Al-Diwaniya slaughter house, Iraq were used...
January 2016: Avicenna Journal of Medical Biotechnology
Atefeh Seyeddokht, Ali Asghar Aslaminejad, Ali Masoudi-Nejad, Mohammadreza Nassiri, Javad Zahiri, Balal Sadeghi
BACKGROUND: Piwi-interacting RNAs (piRNAs) are small non-coding RNAs (ncRNAs), with a length of about 24-32 nucleotides, which have been discovered recently. These ncRNAs play an important role in germline development, transposon silencing, epigenetic regulation, protecting the genome from invasive transposable elements, and the pathophysiology of diseases such as cancer. piRNA identification is challenging due to the lack of conserved piRNA sequences and structural elements. METHODS: To detect piRNAs, an appropriate feature set, including 8 diverse feature groups to encode each RNA was applied...
January 2016: Avicenna Journal of Medical Biotechnology
Javad Baharara, Elaheh Amini, Farideh Namvar
BACKGROUND: Marine organisms provide appreciable source of novel bioactive compounds with pharmacological potential. There is little information in correlation with anti-cancer activities of brittle star. In the present study, anti-neoplastic efficacy of Ophiocoma erinaceus methanol extract against human cervical cancer cells was investigated. METHODS: The HeLa cells were cultured and exposed to brittle star methanol extract for 24 and 48 hr. The anti-proliferative properties were examined by MTT assay and the type of cell death induced was evaluated through morphological changes, flow cytometry, Annexin kit and caspase assay...
January 2016: Avicenna Journal of Medical Biotechnology
Ali Salimi, Mohammad Babashamsi
BACKGROUND: Vascular Endothelial Growth Factor (VEGF) is a coordinate regulator of physiological angiogenesis during embryogenesis, skeletal growth and reproductive functions. There are several types of VEGF, including VEGF165. VEGFs stimulate endothelial cell growth, angiogenesis, and capillary permeability. Low induction temperature is a major factor for expression of the recombinant VEGF165 in soluble form. The purpose of this study was cloning and optimization of soluble vascular endothelial growth factor165 expression in Escherichia coli (E...
January 2016: Avicenna Journal of Medical Biotechnology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"