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Avicenna Journal of Medical Biotechnology

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https://www.readbyqxmd.com/read/28496951/can-aptameric-ligands-specific-to-plasma-coagulation-factor-vii-bind-the-recombinant-form-with-high-affinity-affinity-measurement-by-fluorescence-method
#1
Maryam Tabarzad, Marzieh Jafari, Nastaran Nafissi-Varcheh
BACKGROUND: Among diverse protein purification systems, affinity chromatography is the most attractive one in the purification process of coagulation factors. Coagulation factor VII is a plasma serine protease that has a significant role in natural human hemostasis and its recombinant form such as AryoSeven(™), has been applied in clinical treatment of bleeding disorders. Immunoaffinity chromatography is the purification method of choice that is currently applied in the development of coagulation factor VIIa products...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496950/development-of-flow-cytometry-fluorescent-in-situ-hybridization-flow-fish-method-for-detection-of-pml-rara-chromosomal-translocation-in-acute-promyelocytic-leukemia-cell-line
#2
Fatemeh Zahedipour, Reza Ranjbaran, Abbas Behzad Behbahani, Khalil Tavakol Afshari, Mohammad Ali Okhovat, Gholamhossein Tamadon, Sedigheh Sharifzadeh
BACKGROUND: Acute Promyelocytic Leukemia (APL) is a subclass of acute myeloid leukemia. The chromosomal aberration in 95% of APL cases is t(15; 17) (q22; q21), which prevents cell differentiation. Characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. The goal of this study was to develop a new and powerful Flow- FISH technique to detect the long isoform (L) of PML-RARa fusion transcript in NB4 cell line. METHODS: To achieve the best condition for fixation, two different fixatives including 2% paraformaldehyde and 75% ethanol were used...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496949/the-effect-of-interactions-of-single-nucleotide-polymorphisms-of-apoa1-apoc3-with-food-group-intakes-on-the-risk-of-metabolic-syndrome
#3
Firoozeh Hosseini-Esfahani, Parvin Mirmiran, Maryam S Daneshpour, Azadeh Mottaghi, Fereidoun Azizi
BACKGROUND: The aim of this study was to examine the interaction of dietary food groups and genetic variants of APOA1/APOC3, relative to Metabolic Syndrome (MetS) risk in adults. METHODS: In this matched nested case-control study, 414 MetS subjects and 414 controls were selected from among participants of Tehran Lipid and Glucose Study. Dietary intake was assessed with the use of a valid and reliable semi-quantitative food frequency questionnaire. Single Nucleotide Polymorphisms (SNPs), APOA1 (rs670, -75G>A and rs5069, +83C>T/APOC3 rs5128 C3238>G) were genotyped by the conventional polymerase chain reaction and restriction fragment length polymorphism...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496948/evaluation-of-igy-antibody-as-a-polyspecific-coombs-reagent
#4
Esteban Justo Gutiérrez Calzado, Marlene Toledano Heredia, Jeorge Fernadez Duharte, Amir-Hassan Zarnani
BACKGROUND: During the last twenty years, the extraction of specific egg yolk (IgY) antibodies from the immunized chickens has been accepted as a useful alternative to the immunization of mammals. The aim of the present study was immunizing the chickens with Human Umbilical Cord Serum (HUCS) and the extraction of specific anti-human globulins (IgG, C3b, and C3d) antibodies from egg yolk in order to obtain polyspecific Coombs reagent. METHODS: The novelty of this work was the achievement of a polyclonal reagent through a very cheap alternative method in accordance with all ethical regulations required for obtaining it...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496947/efficient-media-for-high-lipase-production-one-variable-at-a-time-approach
#5
Safoura Soleymani, Houri Alizadeh, Hossein Mohammadian, Emad Rabbani, Fatemeh Moazen, Hamid MirMohammad Sadeghi, Ziaedin Samsam Shariat, Zahra Etemadifar, Mohammed Rabbani
BACKGROUND: Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media composition that is constantly under review by researchers. In this work, for maximum lipase production by Bacillus sp. ZR-5, culture media compositions were optimized using ″one variable at a time″ strategy. METHODS: For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496946/alpha-alumina-nanoparticle-conjugation-to-cysteine-peptidase-a-and-b-an-efficient-method-for-autophagy-induction
#6
Fatemeh Beyzay, Ahmad Zavaran Hosseini, Sara Soudi
BACKGROUND: Autophagy as a cellular pathway facilitates several immune responses against infection. It also eliminates invading pathogens through transferring content between the cytosol and the lysosomal vesicles and contributes to the cross-presentation of exogenous antigens to T lymphocytes via MHC class I pathway. Autophagy induction is one of the main targets for new drugs and future vaccine formulations. Nanoparticles are one of the candidates for autophagy induction. Cysteine Peptidase A (CPA) and Cysteine Peptidase B (CPB) are two members of papain family (Clan CA, family C1) enzyme that have been considered as a virulence factor of Leishmania (L...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496945/gestational-exposure-to-silymarin-increases-susceptibility-of-balb-c-mice-fetuses-to-apoptosis
#7
Mahbobe Gholami, Seyed Adel Moallem, Mohammad Afshar, Leila Etemad, Gholamreza Karimi
BACKGROUND: Silymarin is a flavonolignan that has been the subject of research to evaluate the beneficial properties for decades. Silymarin has been known for its potent cytoprotective, hepatoprotective and antioxidant activities. The goal of the present study was to gain a deeper understanding of possible molecular mechanisms of apoptosis of the injuries induced by silymarin on BALB/c mice fetuses. METHODS: The present experimental study was carried out in virgin female BALB/c mice...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496944/current-state-of-cartilage-tissue-engineering-using-nanofibrous-scaffolds-and-stem-cells
#8
REVIEW
Somaieh Kazemnejad, Manijeh Khanmohammadi, Nafiseh Baheiraei, Shaghayegh Arasteh
Cartilage is an avascular, aneural, and alymphatic connective tissue with a limited capacity caused by low mitotic activity of its resident cells, chondrocytes. Natural repair of full thickness cartilage defects usually leads to the formation of fibrocartilage with lower function and mechanical force compared with the original hyaline cartilage and further deterioration can occur. Tissue engineering and regenerative medicine is a promising strategy to repair bone and articular cartilage defects and rehabilitate joint functions by focusing on the optimal combination of cells, material scaffolds, and signaling molecules...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496943/research-in-iran-an-overview
#9
EDITORIAL
Shahin Akhondzadeh
No abstract text is available yet for this article.
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090280/in-vitro-activity-of-linezolid-in-combination-with-photodynamic-inactivation-against-staphylococcus-aureus-biofilms
#10
Nasim Kashef, Mahboobeh Akbarizare, Mohammad Reza Razzaghi
BACKGROUND: Biofilm infections are a major challenge in medical practice. Bacteria that live in a biofilm phenotype are more resistant to both antimicrobial therapy and host immune responses compared to their planktonic counterparts. So, there is need for new therapeutic strategies to combat these infections. A promising approach [known as Photodynamic Inactivation (PDI)] to kill bacteria growing as biofilms uses light in combination with a photosensitizer to induce a phototoxic reaction which produces reactive oxygen species that can destroy lipids and proteins causing cell death...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090279/association-of-atp-binding-cassette-transporter-a1-abca1-565-c-t-gene-polymorphism-with-hypoalphalipoproteinemia-and-serum-lipids-il-6-and-crp-levels
#11
Mohammad Mahdi Babashamsi, Sohrab Halalkhor, Hamid Moradi Firouzjah, Hadi Parsian, Seyed Farzad Jalali, Mohammad Babashamsi
BACKGROUND: ATP-binding cassette transporter A1 (ABCA1) is a membrane integral protein which plays a vital role in High Density Lipoprotein (HDL) metabolism and exerts a protective effect against Hypoalphalipoproteinemia (HA) by mediation of rate-limiting step in HDL biogenesis. In addition, this protein possesses anti-inflammatory effects by inhibiting the production of some inflammatory cytokines in macrophages. This study investigated the association of ABCA1-565 C/T gene polymorphism with HA and serum lipids, IL-6 and CRP levels...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090278/isolation-and-identification-of-phyllospheric-bacteria-possessing-antimicrobial-activity-from-astragalus-obtusifolius-prosopis-juliflora-xanthium-strumarium-and-hippocrepis-unisiliqousa
#12
Zohreh Mazinani, Marzieh Zamani, Soroush Sardari
BACKGROUND: The widespread utilization of antimicrobial compounds has caused emergence of resistant microorganisms in the world. Hence, the research to probe the products with antimicrobial features has led to finding natural habitats and discovering new pharmaceutical products. METHODS: In this study, an attempt was made to explore the niche of novel habitat to isolate pyllospheric bacteria from the above ground parts (stems and leaves) of Astragalus obtusifolius, Prosopis juliflora, Xanthium strumarium, and Hippocrepis unisiliqousa to evaluate their antimicrobial features...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090277/anti-quorum-sensing-activity-of-substances-isolated-from-wild-berry-associated-bacteria
#13
Suha M Abudoleh, Adel M Mahasneh
BACKGROUND: Quorum Sensing (QS) is a mechanism used by bacteria to determine their physiological activities and coordinate gene expression based on cell to cell signaling. Many bacterial physiological functions are under the regulation of quorum sensing such as virulence, luminescence, motility, sporulation and biofilm formation. The aim of the present study was to isolate and characterize Quorum Sensing Inhibitory (QSI) substances from epiphytic bacteria residing on wild berries surfaces...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090276/overexpression-of-recombinant-human-teriparatide-rhpth-1-34-in-escherichia-coli-an-innovative-gene-fusion-approach
#14
Nahid Bakhtiari, Zahra Amini Bayat, Sepideh Sagharidouz, Mohsen Vaez
BACKGROUND: Parathyroid hormone is an 84-amino acid peptide secreted by the parathyroid glands. Its physiological role is maintenance of normal serum calcium level and bone remodeling. Biological activity of this hormone is related to N-terminal 1-34 amino acids. The recombinant form of hormone (1-34) has been approved for treatment of osteoporosis from 2002. In this study, a novel fusion partner has been developed for preparation of high yield recombinant 1-34 amino acids of hPTH. METHODS: Novel nucleotide cassette designed encoding a chimeric fusion protein comprising of a fusion partner consisting of a His-tag in N-terminal, 53 amino acids belong to Escherichia coli (E...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090275/induction-of-epigenetic-alteration-by-cpuk02-an-ent-kaurenoid-derivative-of-stevioside
#15
Pooneh Mokarram, Zeinab Mohammadi, Saeid Khazayel, Zhang Dayong
BACKGROUND: Dietary polyphenols, such as those found in green tea and red wine, are linked to antitumor activity. They are known to influence many signaling pathways epigenetically within the human body. In this regard, CPUK02 (15-Oxosteviol benzyl ester) is a new ent-kaurenoid derivative of stevioside and exhibits strong anti-cancer activity in vitro and in vivo. Nowadays, the role of epigenetics in cancer has been the subject of intensive study and DNA methylation targeting represents a relevant strategy for cancer treatment...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090274/the-role-of-m2000-as-an-anti-inflammatory-agent-in-toll-like-receptor-2-microrna-155-pathway
#16
Fatemeh Pourgholi, Mahsa Hajivalili, Rasoul Razavi, Shadi Esmaeili, Behzad Baradaran, Ali Akbar Movasaghpour, Sanam Sadreddini, Hamidreza Goodarzynejad, Abbas Mirshafiey, Mehdi Yousefi
BACKGROUND: M2000 is a newly designed and safe Non-Steroidal Anti-Inflammatory Drug (NSAID). The aim of this study was to assess the effects of M2000 on expression levels of Suppressor of Cytokine Signaling-1 (SOCS-1) and Src Homology-2 domain-containing inositol-5'-phosphatase 1 (SHIP1) proteins via Toll-Like Receptor (TLR) 2/microRNA-155 pathway. METHODS: HEK293 TLR2 cell line and Peripheral Blood Mononuclear Cells (PBMCs) were treated by different concentrations of M2000 in MTT assay...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090273/the-effects-of-berberine-and-palmatine-on-efflux-pumps-inhibition-with-different-gene-patterns-in-pseudomonas-aeruginosa-isolated-from-burn-infections
#17
Seyed Sajjad Aghayan, Hamidreza Kalalian Mogadam, Mozhgan Fazli, Davood Darban-Sarokhalil, Seyed Sajjad Khoramrooz, Fereshteh Jabalameli, Somayeh Yaslianifard, Mehdi Mirzaii
BACKGROUND: Related Multidrug Resistance (MDR) to efflux pumps is a significant problem in treating infections caused by Pseudomonas aeruginosa (P. aeruginosa). Plant compounds have been identified as Pump Inhibitors (EPIs). In the current study, the potential effect of Berberine and Palmatine as EPIs were investigated on efflux pump inhibition through focusing on different gene patterns in P. aeruginosa isolated from burn infections. METHODS: All isolates were collected and identified using the standard biochemical tests...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090272/research-in-iran-hopes-and-disappointments
#18
EDITORIAL
Shahin Akhondzadeh
No abstract text is available yet for this article.
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27920889/lack-of-association-between-interleukin-12-gene-polymorphisms-and-recurrent-aphthous-stomatitis
#19
Isaac Firouze Moqadam, Shamsolmoulouk Najafi, Mahsa Mohammadzadeh, Alireza Zare Bidoki, Hila Yousefi, Elham Farhadi, Arghavan Tonekaboni, Ghasem Meighani, Mohsen Mohammadzadeh, Ali Akbar Amirzargar, Nima Rezaei
No abstract text is available yet for this article.
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27920888/development-of-a-high-resolution-melting-analysis-method-for-cyp2c19-17-genotyping-in-healthy-volunteers
#20
Zahra Ghasemi, Mehrdad Hashemi, Mahsa Ejabati, Seyyed Meisam Ebrahimi, Hamidreza Kheiri Manjili, Ali Sharafi, Ali Ramazani
BACKGROUND: Genetic polymorphisms of drug metabolisms by cytochrome P450 (P450s) could affect drug response, attracting particular interest in the pharmacogenetics. Due to the importance of CYP2C19* 17 allele and its capability of super- fast metabolism and also lack of information about distribution of the alleles in Iranian population, this research aimed to use High Resolution Melting (HRM) method compared to PCR-RFLP for genotyping healthy Iranian population. METHODS: Blood samples were collected from 100 healthy Iranian volunteers...
October 2016: Avicenna Journal of Medical Biotechnology
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