Bahare Niknam, Arezou Azizsoltani, Neda Heidari, Samaneh Tokhanbigli, Helia Alavifard, Mahsa Haji Valili, Davar Amani, Hamid Asadzadeh Aghdaei, Seyed Mahmoud Hashemi, Kaveh Baghaei
BACKGROUND: The isolation of Mesenchymal Stem Cells (MSCs) from various tissues is possible, with the umbilical cord emerging as a competitive alternative to bone marrow. In order to fulfill the demands of cell therapy, it is essential to generate stem cells on a clinical scale while minimizing time, cost, and contamination. Here is a simple and effective protocol for isolating MSC from Wharton's Jelly (WJ-MSC) using the explant method with various supplements. METHODS: Utilizing the explant method, small fragments of Wharton's jelly from the human umbilical cord were cultured in a flask...
2024: Avicenna Journal of Medical Biotechnology