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Avicenna Journal of Medical Biotechnology

Lim Ya Chee, Alaistair Cumming
Background: Disruption of the cholinergic neurotransmitter pathway which is important for cognition, memory and learning abilities has been reported in Alzheimer's Disease (AD) patients. The receptors involved include the Cholinergic Receptors Muscarinic ( CHRM ). CHRM2 gene has been associated with intelligence, personality traits, substance dependence and depression. CHRM3 has been found to heterodimerize with CHRM2 . Methods: DNA samples from 240 AD patients with SNPs rs6962027 of CHRM2 gene and rs7511970 of CHRM3 gene were amplified using PCR and genotyped using Restriction Fragment Length Polymorphism (RFLP)...
July 2018: Avicenna Journal of Medical Biotechnology
Neslihan Edeer Karaca, Ezgi Ulusoy Severcan, Burcu Guven, Elif Azarsiz, Guzide Aksu, Necil Kutukculer
Background: The Transmembrane Activator and Calcium modulator ligand Interactor (TACI), encoded by TNFRSF13B/TACI gene, is mutated in some patients with Common Variable Immunodeficiency (CVID) and IgA Deficiency (IgAD). The purpose of the study was to investigate for the first time in Turkish patients the prevalence of TNFRSF13B alterations in CVID, selective and partial IgAD patients. Methods: Forty two CVID, 36 selective IgAD, 34 partial IgAD and 25 healthy controls were included...
July 2018: Avicenna Journal of Medical Biotechnology
Mehdi Aleahmad, Alireza Ghanavatinejad, Mahmood Bozorgmehr, Mohammad-Reza Shokri, Shohreh Nikoo, Maryam Tavakoli, Somaieh Kazemnejad, Fazel Shokri, Amir-Hassan Zarnani
Background: It is more than sixty years that the concept of the fetal allograft and immunological paradox of pregnancy was proposed and in this context, several regulatory networks and mechanisms have been introduced so far. It is now generally recognized that mesenchymal stem cells exert potent immunoregulatory activity. In this study, for the first time, the potential impact of Menstrual blood Stem Cells (MenSCs), as surrogate for endometrial stem cells, on proliferative capacity of CD4+ T cells was tested...
July 2018: Avicenna Journal of Medical Biotechnology
Mahdi Mahmoudi, Masoud Garshasbi, Amir Ashraf-Ganjouei, Ali Javinani, Mahdi Vojdanian, Masoumeh Saafi, Nooshin Ahmadzadeh, Ahmadreza Jamshidi
Background: Ankylosing Spondylitis (AS) is a chronic autoinflammatory Spondyloarthropathy (SpA) which is characterized by sacroiliitis, which progresses to the axial skeleton. It seems that non-Human Leukocyte Antigen (HLA) and also HLA-B27 are associated with the susceptibility and pathogenesis of the disease. The recent Ge-nome-Wide Association Studies (GWASs) have reported intergenic rs6759298 to be associated with AS etiology. The aim of this study was investigation of the rs6759298 polymorphism in Iranian AS patients...
July 2018: Avicenna Journal of Medical Biotechnology
Mohammad Jafar Mahmoudi, Sara Harsini, Elham Farhadi, Mona Hedayat, Mohammad Taghvaei, Maryam Mahmoudi, Maryam Sadr, Nilufar Esfahanian, Ebrahim Nematipour, Keramat Nourijelyani, Ali Akbar Amirzargar, Nima Rezaei
Background: Inflammatory cytokines have been known to be associated with Chronic Heart Failure (CHF). Given the importance of cytokines in the context of the failing heart, the prevalence of Interleukin-2 (IL-2) and Interferon-gamma (IFN-γ) polymorphisms was studied in patients with CHF due to ischemic heart disease in a case-control study. Methods: Fifty-six Iranian patients with CHF were enrolled in this study as the case group and compared with 139 healthy subjects, using polymerase chain reaction with sequence-specific primers method, so as to determine the frequency of alleles, genotypes and haplotypes of IFN-γ (+874 A/T) and IL-2 (-330 G/T, +166 G/T) SNPs...
July 2018: Avicenna Journal of Medical Biotechnology
Homa Farrokhi Karibozorg, Nahid Masoudian, Kioomars Saliminejad, Asghar Ebadifar, Koorosh Kamali, Hamid Reza Khorram Khorshid
Background: Nonsyndromic cleft lip and/or palate (NSCL/P) is the most common orofacial birth defect, often attributed to ethnic and environmental differences. Up to now, linkage analyses and genome-wide association studies have identified several genomic susceptibility regions for NSCL/P. The WNT genes including WNT3 are strong candidates for NSCL/P, since they are involved in regulating mid-face development and upper lip fusion. This study tested association of the WNT3 polymorphisms, rs-3809857 G/T and rs9890413 G/A, with the risk of NSCL/P in a population of Iranian infants...
July 2018: Avicenna Journal of Medical Biotechnology
Diman Fayez, Kioomars Saliminejad, Shiva Irani, Koorosh Kamali, Toktam Memariani, Hamid Reza Khorram Khorshid
Background: Human arylamine N-acetyltransferase 2 (NAT2) gene has a key role in xenobiotic metabolism through the conjugation of acetyl group to xenobiotic substances. NAT2 has been suggested as a susceptibility factor in endometriosis; however, the results of studies have been controversial. In this study, the association of NAT2 polymorphisms with susceptibility to endometriosis was evaluated in an Iranian population. Methods: This is an association study and totally 141 women with diagnosis of endometriosis and 158 healthy women as control group were analyzed for NAT2 gene polymorphisms (C481T, A803G, G857A and G590A) by PCR-RFLP methods...
July 2018: Avicenna Journal of Medical Biotechnology
A S Ragi, P P Leena, K J P Prashob, S M Nair
Background: The purpose of this study was to investigate the sterol profiling and predict the pharmacological potential of marine gastropod Telescopium telescopium (T. telescopium) , collected from mangrove ecosystem in the South west coast of India. Methods: Sterol fractions were separated from the crude lipids using 15% ethyl acetate. Ethyl acetate fractions were dried under ultrahigh purity N2 and analyzed using GC-MS. The biological activity was predicted using the software CLC-Pred; In silico predictions of cytotoxicity for tumor and non-tumor cell lines and PASS...
July 2018: Avicenna Journal of Medical Biotechnology
Marzieh Basirnejad, Azam Bolhassani, Seyed Mehdi Sadat
Background: Hepatitis C (HCV) is known as a serious blood-borne disease that infects millions of people globally. NS3 is a conserved non-structural sequence of hepatitis C virus which has a major role in activating specific CTL responses. As known, there is no effective vaccine against HCV infection, thus it is required to design a specific regimen of vaccination. Recently, the strong immunological properties of Heat shock proteins (Hsps) led to their use as immunomodulators and an antigen carrier for subunit vaccine candidates...
July 2018: Avicenna Journal of Medical Biotechnology
Mahdi Karimi, Farida Behzadian, Hamideh Rouhaninejad, Sanaz Yari
Background: Biosynthetic teriparatide (1-34) (TPD) is a N-terminally truncated version of human parathyroid hormone (hPTH). The recombinant form of this polypeptide has been expressed in Escherichia coli (E. coli) and approved as the first anabolic treatment of osteoporosis in the EU and the USA. Feasibility of expression and secretion of a tag- fused form of TPD into Bacillus subtilis (B. subtilis) was examined due to several advantages of B. subtilis over E. coli in production of recombinant proteins with pharmacological activities...
July 2018: Avicenna Journal of Medical Biotechnology
Mohammadreza Yousefi, Safar Farajnia, Ahad Mokhtarzadeh, Bahman Akbari, Shiva Ahdi Khosroshahi, Mina Mamipour, Hassan Dariushnejad, Vahideh Ahmadzadeh
Background: CD20 is an important cell surface receptor that is used for target therapy of B cell lymphoma and some related blood diseases due to vital function of CD20. In previous studies, a Rituximab based humanized single chain variable fragment (scFv) antibody showed good reactivity against B cell related cancer cells. But this recombinant protein produced Inclusion Bodies (IBs) in Escherichia coli (E. coli ) cytoplasm. The aim of this study was to investigate the effect of coexpression with cytoplasmic chaperones on expression and solubility of humanized anti-CD20 scFv in E...
July 2018: Avicenna Journal of Medical Biotechnology
Oghlniaz Jorjani, Fatemeh Ghaffarifar, Zohreh Sharifi, Abdolhossein Dalimi, Hajar Ziaei-Hezarjaribi, Benyamin Talebi
Background: Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which is an obligate intracellular parasite in the infected host. Individuals who have been recovered from clinical leishmaniasis develop strong immunity against reinfection. DNA vaccines are the new type of vaccines that induce expression of protein eukaryotic cells. DNA vaccines can be stimulated by the cellular and humoral immune responses using one or several genes. Methods: A DNA vaccine containing plasmids encoding the pcLACK+pcTSA genes of Leishmania major (L...
July 2018: Avicenna Journal of Medical Biotechnology
Reza Y Purwoko, Iis Rosliana, Siti Sobariah, Nabila Hermana, Silvani Permatasari, Dewi Wulandari, Puji Sari, Ernie H Purwaningsih, Chaidir, Hans-Joachim Freisleben, Jeanne A Pawitan, Kusmarinah Bramono
Background: Recently, Phosphatidylcholine (PC) has been used as an off-label treatment for lipolysis injection, which is associated with inflammatory reaction due to sodium deoxycholate, an emulsifier, so that inflammation as side effect occurs in those patients. Liposome formulation from soybean lipid was thought to be a better and safer alternative. This study aimed to analyze the mechanism of Liposomal Soybean Phosphatidylcholine (LSPC) extract from Indonesian soybeans (containing 26% PC) to induce Adipose-derived Stem Cells (ASCs) death in vitro ...
July 2018: Avicenna Journal of Medical Biotechnology
Shahin Akhondzadeh
No abstract text is available yet for this article.
July 2018: Avicenna Journal of Medical Biotechnology
Rouhollah Gazor, Mozhgan Eskandari, Alireza Sharafshah, Mohammad Hadi Bahadori, Mohammad Ghasem Golmohammadi, Parvaneh Keshavarz
Background: Exact mechanisms of fetal harm following vitrification are still unknown. This study was conducted to evaluate the cryopreservation impact on the expression of Epidermal Growth Factor Receptor (EGFR) gene in mouse 2-cell and blastocysts. Methods: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-cells and blastocysts after 44-46 and 88-89 hr , respectively. These embryos were divided into two case and control groups. The fresh case group was cryopreserved using cryotop and warmed after 4 mounts...
April 2018: Avicenna Journal of Medical Biotechnology
Hesam Mokhtari, Gilda Eslami, Hengameh Zandi, Amin Dehghan-Banadkouki, Mahmood Vakili
Background: Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen that could be resistant to many antimicrobial agents. Resistance genes can be carried among gram-negative bacteria by integrons. Enzymatic inactivation is the most important mechanism of resistance to aminoglycosides. In this study, the frequencies of two important resistance gene aac(6')-II a and ant(2″)-I, and genes coding integrase I and II, in K. pneumoniae isolates resistant to aminoglycosides were evaluated...
April 2018: Avicenna Journal of Medical Biotechnology
Tahereh Sadeghian-Rizi, Fereshteh Alsahebfosoul, Mohammad Kazemi, Hossein Khanahmad, Ali Jahanian-Najafabadi
Background: Multiple Sclerosis (MS) is the most common cause of neurologic disability in young adults. Recently, the AIRE gene was identified as a genetic risk factor for several autoimmune diseases in genome wide association studies. The aim of this study was to further investigate the possible role of the AIRE gene in susceptibility to MS in Iranian population. Methods: A total of 112 MS patients and 94 ethnically matched controls were included in the study. The Single-Nucleotide Polymorphism (SNP) (rs1800520, C>G) with a global MAF=0...
April 2018: Avicenna Journal of Medical Biotechnology
Mona Hedayat, Mohammad Jafar Mahmoudi, Mohammad Taghvaei, Ebrahim Nematipour, Elham Farhadi, Nilufar Esfahanian, Maryam Mahmoudi, Maryam Sadr, Keramat Nourijelyani, Ali Akbar Amirzargar, Nima Rezaei
Background: Proinflammatory cytokines have been known to be elevated in patients with Chronic Heart Failure (CHF). Given the importance of proinflammatory cytokines in the context of the failing heart, the prevalence of Tumor Necrosis Factor-α (TNF-α), Interleukin (IL)-6 polymorphisms in patients with CHF was studied due to ischemic heart disease. Methods: Forty three patients with ischemic heart failure were enrolled in this study and compared with 140 healthy individuals...
April 2018: Avicenna Journal of Medical Biotechnology
Sayed Mostafa Hosseini, Bahram Mohammad Soltani, Mahmoud Tavallaei, Seyed Javad Mowla, Elham Tafsiri, Abouzar Bagheri, Hamid Reza Khorram Khorshid
Background: The cyclin E2 (CYCE2) is an important regulator in the progression and development of NSCLC, and its ectopic expression promoted the proliferation, invasion, and migration in several tumors, including Non-Small Cell Lung Cancer (NSCLC). However, the upregulation of CYCE2 in NSCLC cells suggested that it has a key role in tumorigenicity. In addition, the RAS family proteins as oncoproteins were activated in many major tumor types and its suitability as the therapeutic target in NSCLC was proposed...
April 2018: Avicenna Journal of Medical Biotechnology
Babak Qasemi-Panahi, Mansoureh Movahedin, Gholamali Moghaddam, Parviz Tajik, Mortaza Koruji, Javad Ashrafi-Helan, Seyed Abbas Rafat
Background: Sheep industry has taken steps toward transforming itself into a more efficient and competitive field. There are many varieties of sheep breeds in the world that each of them serves a useful purpose in the economies of different civilizations. Ghezel sheep is one of the Iranian important breeds that are raised for meat, milk and wool. Field of spermatogonial cell technologies provides tools for genetic improvement of sheep herd and multiple opportunities for research. Spermatogonial cells are the only stem cells capable of transmitting genetic information to future generations...
April 2018: Avicenna Journal of Medical Biotechnology
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