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Avicenna Journal of Medical Biotechnology

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https://www.readbyqxmd.com/read/28706613/major-components-of-metabolic-parameters-and-nutritional-intakes-in-different-genotypes-of-adiponectin-276-g-t-gene-polymorphism-in-non-diabetes-and-non-alcoholic-iranian-fatty-liver-patients
#1
Fatemeh Mohseni, Sahar Moghbelinejad, Reza Najafipour
BACKGROUND: Genetic and environmental factors are both involved in the etiology of Non-Alcoholic Fatty Liver Disease (NAFLD). Among the genetic factors, certain polymorphisms of adiponectin gene are associated with NAFLD. In the current study, we investigated the association between metabolic parameters with different genotypes of adiponectin +276 G>T polymorphism among the Iranian NAFLD patients, and the effect of nutritional intake with development of NAFLD. METHODS: In this study, 75 patients with NAFLD and 76 healthy individuals were enrolled...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706612/polymorphisms-in-the-estrogen-receptor-beta-gene-and-the-risk-of-unexplained-recurrent-spontaneous-abortion
#2
Marzieh Mahdavipour, Saeed Zarei, Ramina Fatemi, Haleh Edalatkhah, Hamed Heidari-Vala, Mahmood Jeddi-Tehrani, Farah Idali
BACKGROUND: Recurrent Spontaneous Abortion (RSA) is caused by multiple genetic and non-genetic factors. Around 50% of the RSA cases have no known etiology and are considered as Unexplained RSA (URSA). Estrogens, via binding to their receptors, play an important role in female reproduction. This study aimed to investigate whether single nucleotide polymorphisms (SNPs; +1082G/A, +1730G/A and rs1256030 C/T) in the estrogen receptor beta (ESR2) gene are associated with susceptibility to URSA in a population of Iranian women...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706611/a-novel-variant-in-the-pah-gene-causing-phenylketonuria-in-an-iranian-pedigree
#3
Elaheh Alavinejad, Seyede Zahra Sajedi, Masoumeh Razipour, Mona Entezam, Neda Mohajer, Aria Setoodeh, Saeed Talebi, Mohammad Keramatipour
BACKGROUND: Phenylalanine hydroxylase (PAH) gene is the well-known causative gene for classic Phenylketonuria (PKU) (OMIM#261600) disease, with more than 500 reported mutations. Through this study, a novel mutation in the PAH gene in an Iranian pedigree with phenylketonuria was introduced. METHODS: A consanguineous family with a 10-year old affected girl was referred for genetic analysis. Mutation screening of all exons and exon-intron boundaries was performed by Sanger sequencing, and mini haplotype analysis was carried out by genotyping of Short Tandem Repeat (STR) and Variable Number Tandem Repeat (VNTR) alleles...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706610/a-novel-variant-of-oct4-entitled-oct4b3-is-expressed-in-human-bladder-cancer-and-astrocytoma-cell-lines
#4
Ensieh M Poursani, Majid Mehravar, Bahram Mohammad Soltani, Seyed Javad Mowla, James E Trosko
BACKGROUND: Alternative splicing is an important mechanism that regulates gene expression and function in human cells. OCT4, a crucial pluripotency marker in embryonic stem/carcinoma cells generates several spliced variants in different cell types and cancers. The expression of OCT4 in cancers has been challenged in many studies. The existence of several OCT4 spliced variants and absence of specific discriminating primers is the main reason of this controversy. Therefore, using specific primers and discriminating OCT4 variants from each other might help to reduce these discrepancies in carcinogenesis and stem cell researches...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706609/distribution-of-class-i-integron-and-smqnr-resistance-gene-among-stenotrophomonas-maltophilia-isolated-from-clinical-samples-in-iran
#5
Mohammadali Malekan, Bahman Tabaraie, Ladan Akhoundtabar, Parviz Afrough, Ava Behrouzi
BACKGROUND: Stenotrophomonas maltophilia (S. maltophilia) is a multiple-antibiotic-resistant opportunistic pathogen that is being isolated with increasing frequency from patients with health-care-associated infections. S. maltophilia is inherently resistant to most of the available antimicrobial agents. Spread of resistant strains has been attributed, in part, to class I integrons. In vitro susceptibility studies have shown trimethoprim-sulfamethoxazole and new floroquinolones as two important agents with activity against these organisms...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706608/the-effects-of-melilotus-officinalis-extract-on-expression-of-daxx-nfkb-and-vegf-genes-in-the-streptozotocin-induced-rat-model-of-sporadic-alzheimer-s-disease
#6
Niloofar Bazazzadegan, Marzieh Dehghan Shasaltaneh, Kioomars Saliminejad, Koorosh Kamali, Mehdi Banan, Hamid Reza Khorram Khorshid
BACKGROUND: Possible mechanisms of Alzheimer Disease (AD) such as inflammation and oxidative stresses in the brain led us to investigate potential AD therapeutics of Melilotus officinalis, an herbal extract, with possible role as an anti-inflammatory and anti-oxidant agent. Among different genes which had important role in Sporadic AD (SAD), three genes including DAXX, NFkB and VEGF have shown significant statistical diversity in the brains of Alzheimer patients. METHODS: These genes were chosen to be investigated for neuroprotective effects of the extract by comparing the expression level in the hippocampus of Sporadic AD (SAD) rat model using quantitative polymerase chain reaction (qPCR) in the treated and untreated groups...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706607/evaluation-of-factors-affecting-size-and-size-distribution-of-chitosan-electrosprayed-nanoparticles
#7
Morteza Abyadeh, Ali Akbar Karimi Zarchi, Mohammad Ali Faramarzi, Amir Amani
BACKGROUND: Size and size distribution of polymeric nanoparticles have important effect on their properties for pharmaceutical application. In this study, Chitosan nanoparticles were prepared by electrospray method (electrohydrodynamic atomization) and parameters that simultaneously affect size and/or size distribution of chitosan nanoparticles were optimized. METHODS: Effect of formulation/processing three independent formulation/processing parameters, namely concentration, flow rate and applied voltage was investigated on particle size and size distribution of generated nanoparticles using a Box-Behnken experimental design...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706606/biosynthesis-of-silver-nanoparticles-using-chlorella-vulgaris-and-evaluation-of-the-antibacterial-efficacy-against-staphylococcus-aureus
#8
Mohammad Soleimani, Maziar Habibi-Pirkoohi
BACKGROUND: It is well documented that Silver Nanoparticles (SNPs) are potent antimicrobial agents. However, little is known about antimicrobial effects of biologically synthesized SNPs at molecular level. In the present study, efficacy of the green microalgae Chlorella vulgaris in biosynthesis of silver nanoparticles and inhibitory effect of the biosynthesized SNPs on growth and virulence of Staphylococcus aureus (S. aureus) were investigated. METHODS: Algal suspension was incubated in the presence of silver nitrate to induce formation of nanoparticles...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706605/embryonic-stem-cell-conditioned-medium-supports-in-vitro-maturation-of-mouse-oocytes
#9
Saber Miraki, Aram Mokarizadeh, Omid Banafshi, Vahideh Assadollahi, Mahdad Abdi, Daem Roshani, Fardin Fathi
BACKGROUND: This study aimed to investigate the maturation and fertilization rates of immature mouse oocytes using Embryonic Stem Cell Conditioned Medium (ESCM). METHODS: Germinal Vesicle (GV) stage oocytes were observed in 120 NMRI mice, aged 4-6 weeks. GV oocytes with or without cumulus cells were subjected to IVM in either ESCM, Embryonic Stem Cell Growth Medium (ESGM), or α-minimum essential medium (α-MEM). After recording the Metaphase II (MII) oocyte maturation rate, the oocytes were fertilized in vitro...
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28706604/innovation-and-technology-in-iran
#10
EDITORIAL
Shahin Akhondzadeh
No abstract text is available yet for this article.
July 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496951/can-aptameric-ligands-specific-to-plasma-coagulation-factor-vii-bind-the-recombinant-form-with-high-affinity-affinity-measurement-by-fluorescence-method
#11
Maryam Tabarzad, Marzieh Jafari, Nastaran Nafissi-Varcheh
BACKGROUND: Among diverse protein purification systems, affinity chromatography is the most attractive one in the purification process of coagulation factors. Coagulation factor VII is a plasma serine protease that has a significant role in natural human hemostasis and its recombinant form such as AryoSeven(™), has been applied in clinical treatment of bleeding disorders. Immunoaffinity chromatography is the purification method of choice that is currently applied in the development of coagulation factor VIIa products...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496950/development-of-flow-cytometry-fluorescent-in-situ-hybridization-flow-fish-method-for-detection-of-pml-rara-chromosomal-translocation-in-acute-promyelocytic-leukemia-cell-line
#12
Fatemeh Zahedipour, Reza Ranjbaran, Abbas Behzad Behbahani, Khalil Tavakol Afshari, Mohammad Ali Okhovat, Gholamhossein Tamadon, Sedigheh Sharifzadeh
BACKGROUND: Acute Promyelocytic Leukemia (APL) is a subclass of acute myeloid leukemia. The chromosomal aberration in 95% of APL cases is t(15; 17) (q22; q21), which prevents cell differentiation. Characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. The goal of this study was to develop a new and powerful Flow- FISH technique to detect the long isoform (L) of PML-RARa fusion transcript in NB4 cell line. METHODS: To achieve the best condition for fixation, two different fixatives including 2% paraformaldehyde and 75% ethanol were used...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496949/the-effect-of-interactions-of-single-nucleotide-polymorphisms-of-apoa1-apoc3-with-food-group-intakes-on-the-risk-of-metabolic-syndrome
#13
Firoozeh Hosseini-Esfahani, Parvin Mirmiran, Maryam S Daneshpour, Azadeh Mottaghi, Fereidoun Azizi
BACKGROUND: The aim of this study was to examine the interaction of dietary food groups and genetic variants of APOA1/APOC3, relative to Metabolic Syndrome (MetS) risk in adults. METHODS: In this matched nested case-control study, 414 MetS subjects and 414 controls were selected from among participants of Tehran Lipid and Glucose Study. Dietary intake was assessed with the use of a valid and reliable semi-quantitative food frequency questionnaire. Single Nucleotide Polymorphisms (SNPs), APOA1 (rs670, -75G>A and rs5069, +83C>T/APOC3 rs5128 C3238>G) were genotyped by the conventional polymerase chain reaction and restriction fragment length polymorphism...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496948/evaluation-of-igy-antibody-as-a-polyspecific-coombs-reagent
#14
Esteban Justo Gutiérrez Calzado, Marlene Toledano Heredia, Jeorge Fernadez Duharte, Amir-Hassan Zarnani
BACKGROUND: During the last twenty years, the extraction of specific egg yolk (IgY) antibodies from the immunized chickens has been accepted as a useful alternative to the immunization of mammals. The aim of the present study was immunizing the chickens with Human Umbilical Cord Serum (HUCS) and the extraction of specific anti-human globulins (IgG, C3b, and C3d) antibodies from egg yolk in order to obtain polyspecific Coombs reagent. METHODS: The novelty of this work was the achievement of a polyclonal reagent through a very cheap alternative method in accordance with all ethical regulations required for obtaining it...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496947/efficient-media-for-high-lipase-production-one-variable-at-a-time-approach
#15
Safoura Soleymani, Houri Alizadeh, Hossein Mohammadian, Emad Rabbani, Fatemeh Moazen, Hamid MirMohammad Sadeghi, Ziaedin Samsam Shariat, Zahra Etemadifar, Mohammed Rabbani
BACKGROUND: Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media composition that is constantly under review by researchers. In this work, for maximum lipase production by Bacillus sp. ZR-5, culture media compositions were optimized using ″one variable at a time″ strategy. METHODS: For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496946/alpha-alumina-nanoparticle-conjugation-to-cysteine-peptidase-a-and-b-an-efficient-method-for-autophagy-induction
#16
Fatemeh Beyzay, Ahmad Zavaran Hosseini, Sara Soudi
BACKGROUND: Autophagy as a cellular pathway facilitates several immune responses against infection. It also eliminates invading pathogens through transferring content between the cytosol and the lysosomal vesicles and contributes to the cross-presentation of exogenous antigens to T lymphocytes via MHC class I pathway. Autophagy induction is one of the main targets for new drugs and future vaccine formulations. Nanoparticles are one of the candidates for autophagy induction. Cysteine Peptidase A (CPA) and Cysteine Peptidase B (CPB) are two members of papain family (Clan CA, family C1) enzyme that have been considered as a virulence factor of Leishmania (L...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496945/gestational-exposure-to-silymarin-increases-susceptibility-of-balb-c-mice-fetuses-to-apoptosis
#17
Mahbobe Gholami, Seyed Adel Moallem, Mohammad Afshar, Leila Etemad, Gholamreza Karimi
BACKGROUND: Silymarin is a flavonolignan that has been the subject of research to evaluate the beneficial properties for decades. Silymarin has been known for its potent cytoprotective, hepatoprotective and antioxidant activities. The goal of the present study was to gain a deeper understanding of possible molecular mechanisms of apoptosis of the injuries induced by silymarin on BALB/c mice fetuses. METHODS: The present experimental study was carried out in virgin female BALB/c mice...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496944/current-state-of-cartilage-tissue-engineering-using-nanofibrous-scaffolds-and-stem-cells
#18
REVIEW
Somaieh Kazemnejad, Manijeh Khanmohammadi, Nafiseh Baheiraei, Shaghayegh Arasteh
Cartilage is an avascular, aneural, and alymphatic connective tissue with a limited capacity caused by low mitotic activity of its resident cells, chondrocytes. Natural repair of full thickness cartilage defects usually leads to the formation of fibrocartilage with lower function and mechanical force compared with the original hyaline cartilage and further deterioration can occur. Tissue engineering and regenerative medicine is a promising strategy to repair bone and articular cartilage defects and rehabilitate joint functions by focusing on the optimal combination of cells, material scaffolds, and signaling molecules...
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28496943/research-in-iran-an-overview
#19
EDITORIAL
Shahin Akhondzadeh
No abstract text is available yet for this article.
April 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28090280/in-vitro-activity-of-linezolid-in-combination-with-photodynamic-inactivation-against-staphylococcus-aureus-biofilms
#20
Nasim Kashef, Mahboobeh Akbarizare, Mohammad Reza Razzaghi
BACKGROUND: Biofilm infections are a major challenge in medical practice. Bacteria that live in a biofilm phenotype are more resistant to both antimicrobial therapy and host immune responses compared to their planktonic counterparts. So, there is need for new therapeutic strategies to combat these infections. A promising approach [known as Photodynamic Inactivation (PDI)] to kill bacteria growing as biofilms uses light in combination with a photosensitizer to induce a phototoxic reaction which produces reactive oxygen species that can destroy lipids and proteins causing cell death...
January 2017: Avicenna Journal of Medical Biotechnology
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