journal
MENU ▼
Read by QxMD icon Read
search

Biomolecules & Therapeutics

journal
https://www.readbyqxmd.com/read/27956717/molecular-signature-that-determines-the-acute-tolerance-of-g-protein-coupled-receptors
#1
Chengchun Min, Xiaohan Zhang, Mei Zheng, Ningning Sun, Sirijan Archarya, Xiaowei Zhang, Kyeong-Man Kim
Desensitization and acute tolerance are terms used to describe the attenuation of receptor responsiveness by prolonged or intermittent exposure to an agonist. Unlike desensitization of G protein-coupled receptors (GPCRs), which is commonly explained by steric hindrance caused by the β-arrestins that are translocated to the activated receptors, molecular mechanisms involved in the acute tolerance of GPCRs remain unclear. Our studies with several GPCRs and related mutants showed that the acute tolerance of GPCRs could occur independently of agonist-induced β-arrestin translocation...
December 16, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27956716/therapeutic-potential-of-medicinal-plants-and-their-constituents-on-lung-inflammatory-disorders
#2
REVIEW
Hyun Pyo Kim, Hyun Lim, Yong Soo Kwon
Acute bronchitis and chronic obstructive pulmonary diseases (COPD) are essentially lung inflammatory disorders. Various plant extracts and their constituents showed therapeutic effects on several animal models of lung inflammation. These include coumarins, flavonoids, phenolics, iridoids, monoterpenes, diterpenes and triterpenoids. Some of them exerted inhibitory action mainly by inhibiting the mitogen-activated protein kinase pathway and nuclear transcription factor-κB activation. Especially, many flavonoid derivatives distinctly showed effectiveness on lung inflammation...
December 16, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27956715/intravenous-administration-of-substance-p-attenuates-mechanical-allodynia-following-nerve-injury-by-regulating-neuropathic-pain-related-factors
#3
Eunkyung Chung, Tae Gyoon Yoon, Sumin Kim, Moonkyu Kang, Hyun Jeong Kim, Youngsook Son
This study aimed to investigate the analgesic effect of substance P (SP) in an animal model of neuropathic pain. An experimental model of neuropathic pain, the chronic constriction injury (CCI) model, was established using ICR mice. An intravenous (i.v.) injection of SP (1 nmole/kg) was administered to the mice to examine the analgesic effects of systemic SP on neuropathic pain. Behavioral testing and immunostaining was performed following treatment of the CCI model with SP. SP attenuated mechanical allodynia in a time-dependent manner, beginning at 1 h following administration, peaking at 1 day post-injection, and decaying by 3 days post-injection...
December 16, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27956714/auranofin-suppresses-plasminogen-activator-inhibitor-2-expression-through-annexin-a5-induction-in-human-prostate-cancer-cells
#4
Dong-Won Shin, Yeo-Jung Kwon, Dong-Jin Ye, Hyoung-Seok Baek, Joo-Eun Lee, Young-Jin Chun
Auranofin has been developed as antirheumatic drugs, which is currently under clinical development for the treatment of chronic lymphocytic leukemia. Previous report showed that auranofin induced apoptosis by enhancement of annexin A5 expression in PC-3 cells. To understand the role of annexin A5 in auranofin-mediated apoptosis, we performed microarray data analysis to study annexin A5-controlled gene expression in annexin A5 knockdown PC-3 cells. Of differentially expressed genes, plasminogen activator inhibitor (PAI)-2 was increased by annexin A5 siRNA confirmed by qRT-PCR and western blot...
December 13, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27956713/characterization-of-a-biflaviolin-synthase-cyp158a3-from-streptomyces-avermitilis-and-its-role-in-the-biosynthesis-of-secondary-metabolites
#5
Young-Ran Lim, Songhee Han, Joo-Hwan Kim, Hyoung-Goo Park, Ga-Young Lee, Thien-Kim Le, Chul-Ho Yun, Donghak Kim
Streptomyces avermitilis produces clinically useful drugs such as avermectins and oligomycins. Its genome contains approximately 33 cytochrome P450 genes and they seem to play important roles in the biosynthesis of many secondary metabolites. The SAV_7130 gene from S. avermitilis encodes CYP158A3; the amino acid sequence of this enzyme has high similarity with that of CYP158A2, a biflaviolin synthase from S. coelicolor A3(2). Recombinant S. avermitilis CYP158A3 was heterologously expressed and purified. It exhibited the typical P450 Soret peak at 447 nm in the reduced CO-bound form...
December 13, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27956712/induction-of-integrin-signaling-by-steroid-sulfatase-in-human-cervical-cancer-cells
#6
Dong-Jin Ye, Yeo-Jung Kwon, Sangyun Shin, Hyoung-Seok Baek, Dong-Won Shin, Young-Jin Chun
Steroid sulfatase (STS) is an enzyme responsible for the hydrolysis of aryl and alkyl sulfates. STS plays a pivotal role in the regulation of estrogens and androgens that promote the growth of hormone-dependent tumors, such as those of breast or prostate cancer. However, the molecular function of STS in tumor growth is still not clear. To elucidate the role of STS in cancer cell proliferation, we investigated whether STS is able to regulate the integrin signaling pathway. We found that overexpression of STS in HeLa cells increases the protein and mRNA levels of integrin β1 and fibronectin, a ligand of integrin α5β1...
December 13, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27871158/platelet-shape-changes-and-cytoskeleton-dynamics-as-novel-therapeutic-targets-for-anti-thrombotic-drugs
#7
REVIEW
Eun-Kyung Shin, Hanseul Park, Ji-Yoon Noh, Kyung-Min Lim, Jin-Ho Chung
Platelets play an essential role in hemostasis through aggregation and adhesion to vascular injury sites but their unnecessary activation can often lead to thrombotic diseases. Upon exposure to physical or biochemical stimuli, remarkable platelet shape changes precede aggregation or adhesion. Platelets shape changes facilitate the formation and adhesion of platelet aggregates, but are readily reversible in contrast to the irrevocable characteristics of aggregation and adhesion. In this dynamic phenomenon, complex molecular signaling pathways and a host of diverse cytoskeleton proteins are involved...
November 25, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27871157/synthetic-cannabinoid-induced-immunosuppression-augments-cerebellar-dysfunction-in-tetanus-toxin-treated-mice
#8
Jaesuk Yun, Sun Mi Gu, Tac-Hyung Lee, Yun Jeong Song, Seonhwa Seong, Young-Hoon Kim, Hye Jin Cha, Kyoung Moon Han, Jisoon Shin, Hokyung Oh, Kikyung Jung, Chiyoung Ahn, Hye-Kyung Park, Hyung Soo Kim
Synthetic cannabinoids are one of most abused new psychoactive substances. The recreational use of abused drug has aroused serious concerns about the consequences of these drugs on infection. However, the effects of synthetic cannabinoid on resistance to tetanus toxin are not fully understood yet. In the present study, we aimed to determine if the administration of synthetic cannabinoids increase the susceptibility to tetanus toxin-induced motor behavioral deficit and functional changes in cerebellar neurons in mice...
November 25, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27871156/kahweol-from-coffee-induces-apoptosis-by-upregulating-activating-transcription-factor-3-in-human-colorectal-cancer-cells
#9
Gwang Hun Park, Hun Min Song, Jin Boo Jeong
Kahweol as a coffee-specific diterpene has been reported to induce apoptosis in human cancer cells. Although some molecular targets for kahweol-mediated apoptosis have been elucidated, the further mechanism for apoptotic effect of kahweol is not known. Activating transcription factor 3 (ATF3) has been reported to be associated with apoptosis in colorectal cancer. The present study was performed to investigate the molecular mechanism by which kahweol stimulates ATF3 expression and apoptosis in human colorectal cancer cells...
November 25, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27871155/protective-role-of-fucoidan-in-cerebral-ischemia-reperfusion-injury-through-inhibition-of-mapk-signaling-pathway
#10
Nan Che, Yijie Ma, Yinhu Xin
Fucoidan has been reported to exhibit various beneficial activities ranging from to antivirus and anticancer properties. However, little information is available about the effects of fucoidan on cerebral ischemia-reperfusion injury (IRI). Our study aimed to explore the effects of fucoidan on cerebral IRI, as well as the underlying mechanisms. Sprague-Dawley (SD) rats were randomly subjected to four groups: Sham, IRI+saline (IRI+S), IRI+80 mg/kg fucoidan (IRI+F80), and IRI+160 mg/kg fucoidan (IRI+F160). Fucoidan (80 mg/kg or 160 mg/kg) was intraperitoneally injected from 7 days before the rats were induced to cerebral IRI model with middle cerebral artery occlusion (MCAO) method...
November 25, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27871154/neuroprotection-of-dexmedetomidine-against-cerebral-ischemia-reperfusion-injury-in-rats-involved-in-inhibition-of-nf-%C3%AE%C2%BAb-and-inflammation-response
#11
Lijun Wang, Haiyan Liu, Ligong Zhang, Gongming Wang, Mengyuan Zhang, Yonghui Yu
Dexmedetomidine is an α2-adrenergic receptor agonist that exhibits a protective effect on ischemia-reperfusion injury of the heart, kidney, and other organs. In the present study, we examined the neuroprotective action and potential mechanisms of dexmedetomidine against ischemia-reperfusion induced cerebral injury. Transient focal cerebral ischemia-reperfusion injury was induced in Sprague-Dawley rats by middle cerebral artery occlusion. After the ischemic insult, animals then received intravenous dexmedetomidine of 1 µg/kg load dose, followed by 0...
November 25, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27829272/galangin-activates-the-erk-akt-driven-nrf2-signaling-pathway-to-increase-the-level-of-reduced-glutathione-in-human-keratinocytes
#12
Susara Ruwan Kumara Madduma Hewage, Mei Jing Piao, Kyoung Ah Kang, Yea Seong Ryu, Pattage Madushan Dilhara Jayatissa Fernando, Min Chang Oh, Jeong Eon Park, Kristina Shilnikova, Yu Jin Moon, Dae O Shin, Jin Won Hyun
Previously, we demonstrated that galangin (3,5,7-trihydroxyflavone) protects human keratinocytes against ultraviolet B (UVB)-induced oxidative damage. In this study, we investigated the effect of galangin on induction of antioxidant enzymes involved in synthesis of reduced glutathione (GSH), and investigated the associated upstream signaling cascades. By activating nuclear factor-erythroid 2-related factor (Nrf2), galangin treatment significantly increased expression of glutamate-cysteine ligase catalytic subunit (GCLC) and glutathione synthetase (GSS)...
November 8, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27829271/ginsenoside-rb1-inhibits-doxorubicin-triggered-h9c2-cell-apoptosis-via-aryl-hydrocarbon-receptor
#13
Yaxin Zhang, Yuguang Wang, Zengchun Ma, Qiande Liang, Xianglin Tang, Hongling Tan, Chengrong Xiao, Yue Gao
Doxorubicin (DOX) is a highly effective chemotherapeutic agent; however, the dose-dependent cardiotoxicity associated with DOX significantly limits its clinical application. In the present study, we investigated whether Rb1 could prevent DOX-induced apoptosis in H9C2 cells via aryl hydrocarbon receptor (AhR). H9C2 cells were treated with various concentrations (- μM) of Rb1. AhR, CYP1A protein and mRNA expression were quantified with Western blot and real-time PCR analyses. We also evaluated the expression levels of caspase-3 to assess the anti-apoptotic effects of Rb1...
November 8, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27829270/biflorin-ameliorates-memory-impairments-induced-by-cholinergic-blockade-in-mice
#14
Se Jin Jeon, Boseong Kim, Byeol Ryu, Eunji Kim, Sunhee Lee, Dae Sik Jang, Jong Hoon Ryu
To examine the effect of biflorin, a component of Syzygium aromaticum, on memory deficit, we introduced a scopolamine-induced cognitive deficit mouse model. A single administration of biflorin increased latency time in the passive avoidance task, ameliorated alternation behavior in the Y-maze, and increased exploration time in the Morris water maze task, indicating the improvement of cognitive behaviors against cholinergic dysfunction. The biflorin-induced reverse of latency in the scopolamine-treated group was attenuated by MK-801, an NMDA receptor antagonist...
November 8, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27829269/tetrandrine-exerts-a-radiosensitization-effect-on-human-glioma-through-inhibiting-proliferation-by-attenuating-erk-phosphorylation
#15
Ji-Wei Ma, Yong Zhang, Ji-Cheng Ye, Ru Li, Yu-Lin Wen, Jian-Xian Huang, Xue-Yun Zhong
Tetrandrine (Tet), a bisbenzylisoquinoline alkaloid, has been reported to have a radiosensitization effect on tumors. However, its effects on human glioma and the specific molecular mechanisms of these effects remain unknown. In this study, we demonstrated that Tet has a radiosensitization effect on human glioma cells. It has been hypothesized that Tet has a radiosensitization effect on glioma cells by affecting the glioma cell cycle and DNA repair mechanism and that ERK mediates these activities. Therefore, we conducted detailed analyses of the effects of Tet on the cell cycle by performing flow cytometric analysis and on DNA repair by detecting the expression of phosphorylated H2AX by immunofluorescence...
November 8, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/27829268/altered-translational-control-of-fmrp-on-myelin-proteins-in-neuropsychiatric-disorders
#16
REVIEW
Se Jin Jeon, Jong Hoon Ryu, Geon Ho Bahn
Myelin is a specialized structure of the nervous system that both enhances electrical conductance and insulates neurons from external risk factors. In the central nervous system, polarized oligodendrocytes form myelin by wrapping processes in a spiral pattern around neuronal axons through myelin-related gene regulation. Since these events occur at a distance from the cell body, post-transcriptional control of gene expression has strategic advantage to fine-tune the overall regulation of protein contents in situ...
November 8, 2016: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28035084/sphingosine-1-phosphate-receptor-modulators-and-drug-discovery
#17
REVIEW
Soo-Jin Park, Dong-Soon Im
Initial discovery on sphingosine 1-phosphate (S1P) as an intracellular second messenger was faced unexpectedly with roles of S1P as a first messenger, which subsequently resulted in cloning of its G protein-coupled receptors, S1P₁₋₅. The molecular identification of S1P receptors opened up a new avenue for pathophysiological research on this lipid mediator. Cellular and molecular in vitro studies and in vivo studies on gene deficient mice have elucidated cellular signaling pathways and the pathophysiological meanings of S1P receptors...
January 1, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28035083/us28-a-virally-encoded-gpcr-as-an-antiviral-target-for-human-cytomegalovirus-infection
#18
REVIEW
Sungjin Lee, Yoon Hee Chung, Choongho Lee
Viruses continue to evolve a new strategy to take advantage of every aspect of host cells in order to maximize their survival. Due to their central roles in transducing a variety of transmembrane signals, GPCRs seem to be a prime target for viruses to pirate for their own use. Incorporation of GPCR functionality into the genome of herpesviruses has been demonstrated to be essential for pathogenesis of many herpesviruses-induced diseases. Here, we introduce US28 of human cytomegalovirus (HCMV) as the beststudied example of virally-encoded GPCRs to manipulate host GPCR signaling...
January 1, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28035082/evolutionary-and-comparative-genomics-to-drive-rational-drug-design-with-particular-focus-on-neuropeptide-seven-transmembrane-receptors
#19
REVIEW
Michael Furlong, Jae Young Seong
Seven transmembrane receptors (7TMRs), also known as G protein-coupled receptors, are popular targets of drug development, particularly 7TMR systems that are activated by peptide ligands. Although many pharmaceutical drugs have been discovered via conventional bulk analysis techniques the increasing availability of structural and evolutionary data are facilitating change to rational, targeted drug design. This article discusses the appeal of neuropeptide-7TMR systems as drug targets and provides an overview of concepts in the evolution of vertebrate genomes and gene families...
January 1, 2017: Biomolecules & Therapeutics
https://www.readbyqxmd.com/read/28035081/%C3%AE-adrenergic-receptor-and-insulin-resistance-in-the-heart
#20
REVIEW
Supachoke Mangmool, Tananat Denkaew, Warisara Parichatikanond, Hitoshi Kurose
Insulin resistance is characterized by the reduced ability of insulin to stimulate tissue uptake and disposal of glucose including cardiac muscle. These conditions accelerate the progression of heart failure and increase cardiovascular morbidity and mortality in patients with cardiovascular diseases. It is noteworthy that some conditions of insulin resistance are characterized by up-regulation of the sympathetic nervous system, resulting in enhanced stimulation of β-adrenergic receptor (βAR). Overstimulation of βARs leads to the development of heart failure and is associated with the pathogenesis of insulin resistance in the heart...
January 1, 2017: Biomolecules & Therapeutics
journal
journal
46750
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"