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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/30177270/direct-identification-of-bovine-mastitis-pathogens-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-in-pre-incubated-milk
#1
Juliana R Barreiro, Juliano L Gonçalves, Rafaella Grenfell, Renata F Leite, Luiz Juliano, Marcos V Santos
The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30174202/the-occurrence-of-aflatoxigenic-aspergillus-spp-in-dairy-cattle-feed-in-southern-brazil
#2
Augusto Carlos Favaretto Variane, Fabiane Cristina Dos Santos, Fausto Fernandes de Castro, Ione Parra Barbosa-Tessmann, Geraldo Tadeu Dos Santos, Magali Soares Dos Santos Pozza
The presence of mycotoxins or related fungi in animal feed is a major problem for animal and human health. Silage and concentrated feed samples were collected from 21 dairy farms in the Western part of Paraná state in Southern Brazil. Water activity and pH of all samples were measured, and each sample was analyzed to check for the presence of aflatoxigenic Aspergillus. Water activity was observed to be lower in the concentrated feed samples. The pH was lower in the silage samples, indicating fermentation processes...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30150084/multi-enzyme-complex-of-white-rot-fungi-in-saccharification-of-lignocellulosic-material
#3
Wilton Soares Cardoso, Paula Viana Queiroz, Gabriella Peterlini Tavares, Fernando Almeida Santos, Filippe Elias de Freitas Soares, Maria Catarina Megumi Kasuya, José Humberto de Queiroz
The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30145265/antifungal-activity-of-nanoemulsions-encapsulating-oregano-origanum-vulgare-essential-oil-in-vitro-study-and-application-in-minas-padr%C3%A3-o-cheese
#4
Carolina M Bedoya-Serna, Gustavo C Dacanal, Andrezza M Fernandes, Samantha C Pinho
The objective of this study was to evaluate the antifungal activity of nanoemulsions encapsulating essential oil of oregano (Origanum vulgare), both in vitro and after application on Minas Padrão cheese. Nanodispersions were obtained by the phase inversion temperature method. Cladosporium sp., Fusarium sp., and Penicillium sp. genera were isolated from cheese samples and used to evaluate antifungal activity. Minimal inhibitory concentrations of non-encapsulated and encapsulated oregano essential oil were determined, and they were influenced by the encapsulation of the essential oil depending on the type of fungus...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30145262/performance-of-rapid-tests-for-carbapenemase-detection-among-brazilian-enterobacteriaceae-isolates
#5
Lisiane Rech Pancotto, Carolina Silva Nodari, Franciéli Pedrotti Rozales, Tatiane Soldi, Carolina Gomes Siqueira, Ana Lúcia Freitas, Afonso Luís Barth
The global emergence of carbapenemases led to the need of developing new methods for their rapid detection. The aim of this study was to evaluate the performance of the rapid tests for carbapenemase-producing and non-producing Enterobacteriaceae. Carbapenem non-susceptible Enterobacteriaceae from a surveillance study submitted to a multiplex real time PCR for carbapenemase detection were included in this study. The isolates were subjected to the rapid phenotypic tests Carba NP, Blue-Carba and Carbapenem Inactivation Method (CIM)...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29937264/ammonia-determines-transcriptional-profile-of-microorganisms-in-anaerobic-digestion
#6
Nan Zhang, Huijuan Peng, Yong Li, Wenxiu Yang, Yuneng Zou, Huiguo Duan
Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscriptomic analysis, the transcriptional profile of microbial community in anaerobic digestion under low (1500mgL-1 ) and high NH4 + (5000mgL-1 ) concentrations, respectively, were revealed. The results showed that high NH4 + concentrations significantly inhibited methane production but facilitated the accumulations of volatile fatty acids...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29891415/shigatoxigenic-and-atypical-enteropathogenic-escherichia-coli-in-fish-for-human-consumption
#7
Marita Vedovelli Cardozo, Clarissa Araújo Borges, Lívia Gerbasi Beraldo, Renato Pariz Maluta, Andressa Souza Pollo, Mariana Monezi Borzi, Luis Fernando Dos Santos, Subhashinie Kariyawasam, Fernando Antônio de Ávila
Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29866608/petroleum-contamination-and-bioaugmentation-in-bacterial-rhizosphere-communities-from-avicennia-schaueriana
#8
Simone Dealtry, Angela Michelato Ghizelini, Leda C S Mendonça-Hagler, Ricardo Moreira Chaloub, Fernanda Reinert, Tácio M P de Campos, Newton C M Gomes, Kornelia Smalla
Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29805073/polycyclic-aromatic-hydrocarbons-degradation-by-marine-derived-basidiomycetes-optimization-of-the-degradation-process
#9
Gabriela A L Vieira, Mariana Juventina Magrini, Rafaella C Bonugli-Santos, Marili V N Rodrigues, Lara D Sette
Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Therefore, this study aimed to select one PAHs degrader among three marine-derived basidiomycete fungi and to study its pyrene detoxification/degradation. Marasmiellus sp. CBMAI 1062 showed higher levels of pyrene and BaP degradation and was subjected to studies related to pyrene degradation optimization using experimental design, acute toxicity, organic carbon removal (TOC), and metabolite evaluation...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29752010/comparison-of-genexpert-mtb-rif-assay-and-led-fm-microscopy-for-the-diagnosis-of-extra-pulmonary-tuberculosis-in-khyber-pakhtunkhwa-pakistan
#10
Anwar Sheed Khan, Sajid Ali, Muhammad Tahir Khan, Sajjad Ahmed, Yasir Khattak, Abduljabbar, Muhammad Irfan, Wasim Sajjad
GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n=390) to 47% (n=347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29728342/statistical-optimization-of-kojic-acid-production-by-a-uv-induced-mutant-strain-of-aspergillus-terreus
#11
Mojtaba Shakibaie, Atefeh Ameri, Roya Ghazanfarian, Mahboubeh Adeli-Sardou, Sahar Amirpour-Rostami, Masoud Torkzadeh-Mahani, Mehdi Imani, Hamid Forootanfar
The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5-40min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10 , 7.63g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KH2 PO4 (NH4 )2 SO4 , and pH) influences on kojic acid production by the C5-10 mutant...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29728341/adopt-a-bacterium-an-active-and-collaborative-learning-experience-in-microbiology-based-on-social-media
#12
Marco Aurélio Floriano Piantola, Ana Carolina Ramos Moreno, Heloísa Alonso Matielo, Natalia Pasternak Taschner, Rafael Ciro Marques Cavalcante, Samia Khan, Rita de Cássia Café Ferreira
The "Adopt a Bacterium" project is based on the use of social network as a tool in Microbiology undergraduate education, improving student learning and encouraging students to participate in collaborative learning. The approach involves active participation of both students and teachers, emphasizing knowledge exchange, based on widely used social media. Students were organized in groups and asked to adopt a specific bacterial genus and, subsequently, submit posts about "adopted genus". The formative assessment is based on posting information on Facebook®, and the summative assessment involves presentation of seminars about the adopted theme...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29728338/carotenoid-production-in-sugarcane-juice-and-synthetic-media-supplemented-with-nutrients-by-rhodotorula-rubra-l02
#13
Mariana de Paula Bonadio, Lidyane Aline de Freita, Márcia Justino Rossini Mutton
In order for the use of biological carotenoids to become feasible, it is necessary to have adequate low cost sources and improved methods of cultivation. The aim of this study was to evaluate the effect of supplementation with nitrogen, phosphorus, zinc, and magnesium, on the biomass and carotenoid volumetric production by yeast Rhodotorula rubra L02 using a complex medium (sugarcane juice) and synthetic media (sucrose and maltose) as substrates. The experimental design used for each substrate was randomized in blocks with 16 treatments and 3 repetitions...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29706575/first-identification-of-clinical-isolate-of-a-novel-ndm-4-producing-escherichia-coli-st405-from-urine-sample-in-pakistan
#14
LETTER
Muhammad Usman Qamar, Timothy R Walsh, Mark A Toleman, Sidrah Saleem, Shah Jahan
No abstract text is available yet for this article.
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29705164/saccharomyces-cerevisiae-populations-and-other-yeasts-associated-with-indigenous-beers-chicha-of-ecuador
#15
Fernanda Barbosa Piló, Enrique Javier Carvajal-Barriga, Maria Cristina Guamán-Burneo, Patricia Portero-Barahona, Arthur Matoso Morato Dias, Larissa Falabella Daher de Freitas, Fátima de Cássia Oliveira Gomes, Carlos Augusto Rosa
Chicha, a type of beer made mainly with maize or cassava, is a traditional fermented beverage of the Andean region. There have only been a few studies on yeasts associated with chicha fermentation, and the species diversity occurring during the production of this beverage is not known. The objective of this study was to determine the biodiversity of yeasts in chicha, and to characterize the Saccharomyces cerevisiae populations associated with the production of chicha de jora, seven-grain chicha, chicha de yuca, and chicha de morocho in Ecuador...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29705163/free-fatty-acids-reduce-metabolic-stress-and-favor-a-stable-production-of-heterologous-proteins-in-pichia-pastoris
#16
Andrea B Zepeda, Carolina A Figueroa, Adalberto Pessoa, Jorge G Farías
The growth of yeasts in culture media can be affected by many factors. For example, methanol can be metabolized by other pathways to produce ethanol, which acts as an inhibitor of the heterologous protein production pathway; oxygen concentration can generate aerobic or anaerobic environments and affects the fermentation rate; and temperature affects the central carbon metabolism and stress response protein folding. The main goal of this study was determine the implication of free fatty acids on the production of heterologous proteins in different culture conditions in cultures of Pichia pastoris...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29705162/streptomyces-globosus-dk15-and-streptomyces-ederensis-st13-as-new-producers-of-factumycin-and-tetrangomycin-antibiotics
#17
Ivana Charousová, Juraj Medo, Lukáš Hleba, Soňa Javoreková
Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29703526/changes-induced-by-co-inoculation-in-nitrogen-carbon-metabolism-in-cowpea-under-salinity-stress
#18
Alexandra de Andrade Santos, Joaquim Albenísio Gomes da Silveira, Eliezer de Araujo Guilherme, Aurenivia Bonifacio, Artenisa Cerqueira Rodrigues, Márcia do Vale Barreto Figueiredo
To mitigate the deleterious effects of abiotic stress, the use of plant growth-promoting bacteria along with diazotrophic bacteria has been increasing. The objectives of this study were to investigate the key enzymes related to nitrogen and carbon metabolism in the biological nitrogen fixation process and to elucidate the activities of these enzymes by the synergistic interaction between Bradyrhizobium and plant growth-promoting bacteria in the absence and presence of salt stress. Cowpea plants were cultivated under axenic conditions, inoculated with Bradyrhizobium and co-inoculated with Bradyrhizobium sp...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29691193/cloning-and-high-level-expression-of-thermus-thermophilus-reca-in-e-coli-purification-and-novel-use-in-hbv-diagnostics
#19
Sudarson Sundarrajan, Sneha Rao, Sriram Padmanabhan
We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was purified using a single heat treatment step without the use of any chromatography steps, and the purified protein (>95%) was found to be active. The purified RecA could enhance the polymerase chain reaction (PCR) signals of HBV and improve the detection limit of the HBV diagnosis by real time PCR...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29691192/identification-of-pathogenic-and-nonpathogenic-leptospira-species-of-brazilian-isolates-by-matrix-assisted-laser-desorption-ionization-and-time-flight-mass-spectrometry
#20
Daniel Karcher, Rafaella C Grenfell, Andrea Micke Moreno, Luisa Zanolli Moreno, Silvio Arruda Vasconcellos, Marcos B Heinemann, Joao N de Almeida Junior, Luiz Juliano, Maria A Juliano
Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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