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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/28283415/fermentation-process-for-production-of-apple-based-kefir-vinegar-microbiological-chemical-and-sensory-analysis
#1
Roberta Oliveira Viana, Karina Teixeira Magalhães-Guedes, Roberto Alves Braga, Disney Ribeiro Dias, Rosane Freitas Schwan
The aim of this study was to develop a kefir apple-based vinegar and evaluate this fermentation process using new methodology with Biospeckle Laser. Brazilian kefir grains were inoculated in apple must for vinegar production. In this study, the microbial community present in kefir, and correspondent vinegar, was investigated using Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) technique. Saccharomyces cerevisiae, Lactobacillus paracasei, Lactobacillus plantarum, Acetobacter pasteurianus and Acetobacter syzygii were the microbial species identified...
March 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28292630/a-plate-method-for-rapid-screening-of-ketogulonicigenium-vulgare-mutants-for-enhanced-2-keto-l-gulonic-acid-production
#2
Weichao Yang, Litao Han, Mandlaa Mandlaa, Haihong Zhang, Zhongze Zhang, Hui Xu
A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94...
February 21, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28279600/development-of-a-novel-compound-microbial-agent-for-degradation-of-kitchen-waste
#3
Kaining Zhao, Rui Xu, Ying Zhang, Hao Tang, Chuanbin Zhou, Aixin Cao, Guozhu Zhao, Hui Guo
Large quantities of kitchen waste are produced in modern society and its disposal poses serious environmental and social problems. The aim of this study was to isolate degradative strains from kitchen waste and to develop a novel and effective microbial agent. One hundred and four strains were isolated from kitchen waste and the 84 dominant strains were used to inoculate protein-, starch-, fat- and cellulose-containing media for detecting their degradability. Twelve dominant strains of various species with high degradability (eight bacteria, one actinomycetes and three fungi) were selected to develop a compound microbial agent "YH" and five strains of these species including H7 (Brevibacterium epidermidis), A3 (Paenibacillus polymyxa), E3 (Aspergillus japonicus), F9 (Aspergillus versicolor) and A5 (Penicillium digitatum), were new for kitchen waste degradation...
February 21, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28320598/molecular-characterization-of-ureaplasmas-isolated-from-reproductive-tract-of-goats-and-sheep-from-brazil
#4
Rosângela C Oliveira, Maurício Yamaguti, Lucas M Marques, Melissa Buzinhani, Huber Rizzo, Enoch Brandão de Souza Meira-Junior, Ana Márcia S Guimarães, Lilian Gregory, Jorge Timenetsky
Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility...
February 20, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28279601/enhanced-ethanol-production-at-commercial-scale-from-molasses-using-high-gravity-technology-by-mutant-s-cerevisiae
#5
Muhammad Arshad, Tariq Hussain, Munawar Iqbal, Mazhar Abbas
Very high gravity (VHG) technology was employed on industrial scale to produce ethanol from molasses (fermented) as well as by-products formation estimation. The effect of different Brix° (32, 36 and 40) air-flow rates (0.00, 0.20, 0.40, and 0.60vvm) was studied on ethanol production. The maximum ethanol production was recorded to be 12.2% (v/v) at 40 Brix° with 0.2vvm air-flow rate. At optimum level aeration and 40 Brix° VHG, the residual sugar level was recorded in the range of 12.5-18.5g/L, whereas the viable cell count remained constant up to 50h of fermentation and dry matter production increased with fermentation time...
February 16, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28262388/enzymatic-comparison-and-mortality-of-beauveria-bassiana-against-cabbage-caterpillar-pieris-brassicae-linn
#6
Manish Dhawan, Neelam Joshi
Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (10(9)conidia/ml), and the minimum mortality (30...
February 16, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28256391/molecular-epidemiology-of-streptococcus-agalactiae-isolated-from-mastitis-in-brazilian-dairy-herds
#7
Glei A Carvalho-Castro, Juliana R Silva, Luciano V Paiva, Dircéia A C Custódio, Rafael O Moreira, Glaucia F Mian, Ingrid A Prado, Antônio Chalfun-Junior, Geraldo M Costa
Streptococcus agalactiae is one of the most common pathogens leading to mastitis in dairy herds worldwide; consequently, the pathogen causes major economic losses for affected farmers. In this study, multilocus sequence typing (MLST), genotypic capsular typing by multiplex polymerase chain reaction (PCR), and virulence gene detection were performed to address the molecular epidemiology of 59 bovine (mastitis) S. agalactiae isolates from 36 dairy farms located in the largest milk-producing mesoregions in Brazil (Minas Gerais, São Paulo, Paraná, and Pernambuco)...
February 16, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28245966/immobilization-of-ammonia-oxidizing-bacteria-by-polyvinyl-alcohol-and-sodium-alginate
#8
Yuwei Dong, Yanqiu Zhang, Baojun Tu
Ammonia-oxidizing bacteria were immobilized by polyvinyl alcohol (PVA) and sodium alginate. The immobilization conditions and ammonia oxidation ability of the immobilized bacteria were investigated. The following immobilization conditions were observed to be optimal: PVA, 12%; sodium alginate, 1.1%; calcium chloride, 1.0%; inoculum concentration, 1.3 immobilized balls/mL of immobilized medium; pH, 10; and temperature, 30°C. The immobilized ammonia-oxidizing bacteria exhibited strong ammonia oxidation ability even after being recycled four times...
February 15, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28245965/prevalence-serotyping-and-antimicrobials-resistance-mechanism-of-salmonella-enterica-isolated-from-clinical-and-environmental-samples-in-saudi-arabia
#9
Mohamed A El-Tayeb, Abdelnasser S S Ibrahim, Ali A Al-Salamah, Khalid S Almaary, Yahya B Elbadawi
Salmonella is recognized as a common foodborne pathogen, causing major health problems in Saudi Arabia. Herein, we report epidemiology, antimicrobial susceptibility and the genetic basis of resistance among S. enterica strains isolated in Saudi Arabia. Isolation of Salmonella spp. from clinical and environmental samples resulted in isolation of 33 strains identified as S. enterica based on their biochemical characteristics and 16S-rDNA sequences. S. enterica serovar Enteritidis showed highest prevalence (39...
February 14, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28291698/detection-and-analysis-of-different-interactions-between-resistance-mechanisms-and-carbapenems-in-clinical-isolates-of-klebsiella-pneumoniae
#10
Tanise Vendruscolo Dalmolin, Bianca Vendruscolo Bianchini, Grazielle Guidolin Rossi, Ana Carolina Ramos, Ana Cristina Gales, Priscila de Arruda Trindade, Marli Matiko Anraku de Campos
Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28237679/pectin-lyase-overproduction-by-penicillium-griseoroseum-mutants-resistant-to-catabolite-repression
#11
Juliana Oliveira Lima, Jorge Fernando Pereira, Elza Fernandes de Araújo, Marisa Vieira de Queiroz
Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants were obtained. Some mutants produced sectors (segments morphologically different) that were also studied. The mutants were analyzed for pectinases production on pectinase-agar plates and five mutants and two sectors showing larger clearing zones than the wild type were selected for quantitative assay...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28237678/cultivation-of-pichia-pastoris-carrying-the-scfv-anti-ldl-antibody-fragment-effect-of-preculture-carbon-source
#12
Cesar Andres Diaz Arias, Daniela de Araujo Viana Marques, Luciana Pellegrini Malpiedi, Andrea Queiroz Maranhão, Dulcineia Abdalla Saes Parra, Attilio Converti, Adalberto Pessoa Junior
Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment anti low density lipoprotein (-) under the control of the alcohol oxidase promoter have been investigated in this study. In particular, it was evaluated if, and eventually how, the carbon source (glucose or glycerol) used in the preculture preceding cryopreservation in 20% glycerol influences both cell and antibody fragment productions either in flasks or in bioreactor...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28237677/growth-viability-and-architecture-of-biofilms-of-listeria-monocytogenes-formed-on-abiotic-surfaces
#13
Fernanda Barbosa Dos Reis-Teixeira, Virgínia Farias Alves, Elaine Cristina Pereira de Martinis
The pathogenic bacterium Listeria monocytogenes can persist in food processing plants for many years, even when appropriate hygienic measures are in place, with potential for contaminating ready-to-eat products and, its ability to form biofilms on abiotic surfaces certainly contributes for the environmental persistence. In this research, L. monocytogenes was grown in biofilms up 8 days attached to stainless steel and glass surfaces, contributing for advancing the knowledge on architecture of mature biofilms, since many literature studies carried out on this topic considered only early stages of cell adhesion...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28237676/in-vitro-antifungal-activity-of-organic-compounds-derived-from-amino-alcohols-against-onychomycosis
#14
César Augusto Caneschi, Angelina Maria de Almeida, Francislene Juliana Martins, Mireille Le Hyaric, Manoel Marques Evangelista Oliveira, Gilson Costa Macedo, Mauro Vieira de Almeida, Nádia Rezende Barbosa Raposo
Onychomycosis is a fungal infection of the nail caused by high densities of filamentous fungi and yeasts. Treatment for this illness is long-term, and recurrences are frequently detected. This study evaluated in vitro antifungal activities of 12 organic compounds derived from amino alcohols against standard fungal strains, such as Trichophyton rubrum CCT 5507 URM 1666, Trichophyton mentagrophytes ATCC 11481, and Candida albicans ATCC 10231. The antifungal compounds were synthesized from p-hydroxybenzaldehyde (4a-4f) and p-hydroxybenzoic acid (9a-9f)...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28237675/draft-genome-sequence-of-bradyrhizobium-manausense-strain-br-3351-t-an-effective-symbiont-isolated-from-amazon-rainforest
#15
Jean Luiz Simões-Araújo, Norma Gouvêa Rumjanek, Gustavo Ribeiro Xavier, Jerri Édson Zilli
The strain BR 3351(T) (Bradyrhizobium manausense) was obtained from nodules of cowpea (Vigna unguiculata L. Walp) growing in soil collected from Amazon rainforest. Furthermore, it was observed that the strain has high capacity to fix nitrogen symbiotically in symbioses with cowpea. We report here the draft genome sequence of strain BR 3351(T). The information presented will be important for comparative analysis of nodulation and nitrogen fixation for diazotrophic bacteria. A draft genome with 9,145,311bp and 62...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28237674/endophytic-fungus-purpureocillium-sp-a5-protect-mangrove-plant-kandelia-candel-under-copper-stress
#16
Bin Gong, Guixiang Liu, Rquan Liao, Jingjing Song, Hong Zhang
Mangrove is an important ecosystem in the world. Mangrove ecosystems have a large capacity in retaining heavy metals, and now they are usually considered as sinks for heavy metals. However, the mechanism of why the soil of mangrove ecosystems can retain heavy metal is not certain. In this research, endophytic fungus Purpureocillium sp. A5 was isolated and identified from the roots of Kandelia candel. When this fungus was added, it protected the growth of K. candel under Cu stress. This can be illustrated by analyzing chlorophyll A and B, RWC and WSD to leaves of K...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28223028/short-interfering-rnas-targeting-a-vampire-bat-related-rabies-virus-phosphoprotein-mrna
#17
Ekaterina Alexandrovna Durymanova Ono, Sueli Akemi Taniwaki, Paulo Brandão
The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1log decrease in virus titter and 5...
February 5, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28256390/whole-genome-sequence-of-lactic-acid-bacterium-pediococcus-acidilactici-strain-s1
#18
Gun-Seok Park, Sung-Jun Hong, Byung Kwon Jung, Seulki Park, Hyewon Jin, Sang-Jae Lee, Jae-Ho Shin, Han-Seung Lee
Pediococcus acidilactici strain S1, a lactic acid-fermenting bacterium, was isolated from makgeolli-a Korean traditional fermented alcoholic beverage. Here we report the 1,980,172bp (G+C content, 42%) genome sequence of Pediococcus acidilactici strain S1 with 1,525 protein-coding sequences (CDS), of which 47% could be assigned to recognized functional genes. The genome sequence of the strain S1 might provide insights into the genetic basis of the lactic acid bacterium with alcohol-tolerant.
February 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28223030/heterologous-expression-of-a-rice-metallothionein-isoform-osmti-1b-in-saccharomyces-cerevisiae-enhances-cadmium-hydrogen-peroxide-and-ethanol-tolerance
#19
Zahra Ansarypour, Azar Shahpiri
Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd(2+), H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S...
February 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28223029/new-production-process-of-the-antifungal-chaetoglobosin-a-using-cornstalks
#20
Cheng Jiang, Jinzhu Song, Junzheng Zhang, Qian Yang
Chaetoglobosin A is an antibacterial compound produced by Chaetomium globosum, with potential application as a biopesticide and cancer treatment drug. The aim of this study was to evaluate the feasibility of utilizing cornstalks to produce chaetoglobosin A by C. globosum W7 in solid-batch fermentation and to determine an optimal method for purification of the products. The output of chaetoglobosin A from the cornstalks was 0.34mg/g, and its content in the crude extract was 4.80%. Purification conditions were optimized to increase the content of chaetoglobosin A in the crude extract, including the extract solvent, temperature, and pH value...
February 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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