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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/28927873/genome-sequence-of-streptomyces-mangrovisoli-musc-149-t-isolated-from-intertidal-sediments
#1
Hooi-Leng Ser, Wen-Si Tan, Nurul-Syakima Ab Mutalib, Wai-Fong Yin, Kok-Gan Chan, Bey-Hing Goh, Learn-Han Lee
As the largest genus in Actinobacteria family, Streptomyces species have the ability to synthesize numerous compounds of diverse structures with bioactivities. Streptomyces mangrovisoli MUSC 149(T) was previously isolated as a novel streptomycete from mangrove forest in east coast of Peninsular Malaysia. The high quality draft genome of MUSC 149(T) comprises 9,165,825bp with G+C content of 72.5%. Through bioinformatics analysis, 21 gene clusters identified in the genome were associated with the production of bioactive secondary metabolites...
September 6, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28888829/sensitivity-specificity-and-comparison-of-three-commercially-available-immunological-tests-in-the-diagnosis-of-cryptosporidium-species-in-animals
#2
Olga Danišová, Monika Halánová, Alexandra Valenčáková, Lenka Luptáková
The study was conducted to compare the specificity of immunological diagnostic methods used for the diagnosis of Cryptosporidium species capable of causing life-threatening infection in both immunosuppressed and immunocompetent patients. For the detection of Cryptosporidium species in 79 animals with diarrhoea, we used three Copro-antigen tests: RIDASCREEN(®)Cryptosporidium test, Cryptosporidium 2nd Generation (ELISA) and RIDA(®)QUICK Cryptosporidium. For immunoassays we used positive and negative samples detected by means of polymerase chain reaction and validated by sequencing and nested polymerase chain reaction to confirm the presence six different species of Cryptosporidium species...
September 6, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28887008/physiological-response-of-cucurbita-pepo-var-pepo-mycorrhized-by-sonoran-desert-native-arbuscular-fungi-to-drought-and-salinity-stresses
#3
Citlalli Harris-Valle, Martín Esqueda, Aldo Gutiérrez, Alejandro E Castellanos, Alfonso A Gardea, Ricardo Berbara
Plants response to symbiosis with arbuscular mycorrhizal fungi (AMF) under water stress is important to agriculture. Under abiotic stress conditions native fungi are more effective than exotics in improving plant growth and water status. Mycorrhization efficiency is related to soil fungi development and energy cost-benefit ratio. In this study, we assessed the effect on growth, water status and energy metabolism of Cucurbita pepo var. pepo when inoculated with native AMF from the Sonoran desert Mexico (mixed isolate and field consortium), and compared with an exotic species from a temperate region, under drought, low and high salinity conditions...
September 5, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28916389/removal-and-killing-of-multispecies-endodontic-biofilms-by-n-acetylcysteine
#4
Young-Suk Choi, Cheul Kim, Ji-Hoi Moon, Jin-Yong Lee
Removal of bacterial biofilm from the root canal system is essential for the management of endodontic disease. Here we evaluated the antibacterial effect of N-acetylcysteine (NAC), a potent antioxidant and mucolytic agent, against mature multispecies endodontic biofilms consisting of Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans and Enterococcus faecalis on sterile human dentin blocks. The biofilms were exposed to NAC (25, 50 and 100mg/mL), saturated calcium hydroxide or 2% chlorhexidine solution for 7 days, then examined by scanning electron microscopy...
September 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28927874/zika-detection-comparison-of-methodologies
#5
Tatiana Elias Colombo, Ana Carolina Bernardes Terzian, João Pessoa Araújo Júnior, Ricardo Parreira, Eliana Márcia Sotello Cabrera, Izalco Nuremberg Penha Dos Santos, Andréia Francesli Negri Reis, Fabiana Rodrigues Costa, Lilian Elisa Arão Antônio Cruz, Patrícia Lopes Rombola, Maurício Lacerda Nogueira
Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan(®) RT-qPCR assay. We used a SYBR(®) Green RT-qPCR and a conventional PCR methodologies to compare the results...
August 30, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28888830/the-isolation-of-pentose-assimilating-yeasts-and-their-xylose-fermentation-potential
#6
Gisele Marta Martins, Daniela Alonso Bocchini-Martins, Carolina Bezzerra-Bussoli, Fernando Carlos Pagnocca, Maurício Boscolo, Diego Alves Monteiro, Roberto da Silva, Eleni Gomes
For the implementation of cellulosic ethanol technology, the maximum use of lignocellulosic materials is important to increase efficiency and to reduce costs. In this context, appropriate use of the pentose released by hemicellulose hydrolysis could improve de economic viability of this process. Since the Saccharomyces cerevisiae is unable to ferment the pentose, the search for pentose-fermenting microorganisms could be an alternative. In this work, the isolation of yeast strains from decaying vegetal materials, flowers, fruits and insects and their application for assimilation and alcoholic fermentation of xylose were carried out...
August 26, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28887007/yersinia-pestis-detection-by-loop-mediated-isothermal-amplification-combined-with-magnetic-bead-capture-of-dna
#7
Na Feng, Yazhou Zhou, Yanxiao Fan, Yujing Bi, Ruifu Yang, Yusen Zhou, Xiaoyi Wang
We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23CFU for pure culture, whereas 2.3×10(4) or 2.3×10(6)CFU for simulated spleen and lung samples. For simulated liver samples, the sensitivity of LAMP was 2.3×10(6)CFU, but PCR was negative at the level of 2...
August 26, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28899604/clinical-and-epidemiological-use-of-nested-pcr-targeting-the-repetitive-element-is1111-associated-with-the-transposase-gene-from-coxiella-burnetii
#8
Maria Angélica M M Mares-Guia, Alexandro Guterres, Tatiana Rozental, Michelle Dos Santos Ferreira, Elba R S Lemos
Q fever is a worldwide zoonosis caused by Coxiella burnetii-a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of testing while offering a suitable amplicon size for sequencing. Serial dilutions were performed tenfold to test the limit of detection, and the result was 10× detection of C...
August 24, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28888828/contribution-of-dark-septate-fungi-to-the-nutrient-uptake-and-growth-of-rice-plants
#9
Carlos Vergara, Karla Emanuelle Campos Araujo, Luiziene Soares Alves, Sônia Regina de Souza, Leandro Azevedo Santos, Claudete Santa-Catarina, Krisle da Silva, Gilmara Maria Duarte Pereira, Gustavo Ribeiro Xavier, Jerri Édson Zilli
The use of dark septate fungi (DSE) to promote plant growth can be beneficial to agriculture, and these organisms are important allies in the search for sustainable agriculture practices. This study investigates the contribution of dark septate fungi to the absorption of nutrients by rice plants and their ensuing growth. Four dark septate fungi isolates that were identified by Internal transcribed spacer phylogeny were inoculated in rice seeds (Cv. Piauí). The resulting root colonization was estimated and the kinetic parameters Vmax and Km were calculated from the nitrate contents of the nutrient solution...
August 23, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28870433/antibacterial-activity-of-3-3-4-trihydroxyflavone-from-justicia-wynaadensis-against-diabetic-wound-and-urinary-tract-infection
#10
Dorin Dsouza, Lakshmidevi Nanjaiah
The present investigation was designed to study the effect of an active compound isolated from Justicia wynaadensis against multi drug resistant organisms (MDRO's) associated with diabetic patients. The drug resistant pathogens implicated in wound and urinary tract infection of diabetic patients were isolated and identified by molecular sequencing. Solvent-solvent fractionation of crude methanol extract produced hexane, chloroform, ethyl acetate and methanol-water fraction, among which chloroform fraction was found to be potent when compared with other three fractions...
August 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28847541/inactivation-of-marr-gene-homologs-increases-susceptibility-to-antimicrobials-in-bacteroides-fragilis
#11
Clara Maria Guimarães Silva, Déborah Nascimento Dos Santos Silva, Scarlathe Bezerra da Costa, Juliana Soares de Sá Almeida, Renata Ferreira Boente, Felipe Lopes Teixeira, Regina Maria Cavalcanti Pilotto Domingues, Leandro Araujo Lobo
Bacteroides fragilis is the strict anaerobic bacteria most commonly found in human infections, and has a high mortality rate. Among other virulence factors, the remarkable ability to acquire resistance to a variety of antimicrobial agents and to tolerate nanomolar concentrations of oxygen explains in part their success in causing infection and colonizing the mucosa. Much attention has been given to genes related to multiple drug resistance derived from plasmids, integrons or transposon, but such genes are also detected in chromosomal systems, like the mar (multiple antibiotic resistance) locus, that confer resistance to a range of drugs...
August 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28844884/effect-of-sulfonylurea-tribenuron-methyl-herbicide-on-soil-actinobacteria-growth-and-characterization-of-resistant-strains
#12
Kounouz Rachedi, Ferial Zermane, Radja Tir, Fatima Ayache, Robert Duran, Béatrice Lauga, Solange Karama, Maryse Simon, Abderrahmane Boulahrouf
Repeated application of pesticides disturbs microbial communities and cause dysfunctions on soil biological processes. Granstar(®) 75 DF is one of the most used sulfonylurea herbicides on cereal crops; it contains 75% of tribenuron-methyl. Assessing the changes on soil microbiota, particularly on the most abundant bacterial groups, will be a useful approach to determine the impact of Granstar(®) herbicide. For this purpose, we analyzed Actinobacteria, which are known for their diversity, abundance, and aptitude to resist to xenobiotic substances...
August 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28844883/heavy-metal-tolerance-traits-of-filamentous-fungi-isolated-from-gold-and-gemstone-mining-sites
#13
Oluwatosin Gbemisola Oladipo, Olusegun Olufemi Awotoye, Akinyemi Olayinka, Cornelius Carlos Bezuidenhout, Mark Steve Maboeta
Increased environmental pollution has necessitated the need for eco-friendly clean-up strategies. Filamentous fungal species from gold and gemstone mine site soils were isolated, identified and assessed for their tolerance to varied heavy metal concentrations of cadmium (Cd), copper (Cu), lead (Pb), arsenic (As) and iron (Fe). The identities of the fungal strains were determined based on the internal transcribed spacer 1 and 2 (ITS 1 and ITS 2) regions. Mycelia growth of the fungal strains were subjected to a range of (0-100 Cd), (0-1000 Cu), (0-400 Pb), (0-500 As) and (0-800 Fe) concentrations (mgkg(-1)) incorporated into malt extract agar (MEA) in triplicates...
August 8, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28843807/a-comparative-study-of-procedures-for-binding-of-aflatoxin-m1-to-lactobacillus-rhamnosus-gg
#14
Jean Claude Assaf, Ali Atoui, André El Khoury, Ali Chokr, Nicolas Louka
Several strains of lactic acid bacteria (LAB), frequently used in food fermentation and preservation, have been reported to bind different types of toxins in liquid media. This study was carried out to investigate the effect of different concentrations of Lactobacillus rhamnosus GG (ATCC 53103) to bind aflatoxin M1 (AFM1) in liquid media. AFM1 binding was tested following repetitive washes or filtration procedures in combination with additional treatments such as heating, pipetting, and centrifugation. The mixture of L...
August 6, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28838812/polyphasic-characterization-of-bacteria-obtained-from-upland-rice-cultivated-in-cerrado-soil
#15
Lívia Fabiana Braga, Fênix Araújo de Oliveira, Eva Aparecida Prado do Couto, Karina Freire d'Eça Nogueira Santos, Enderson Petrônio de Brito Ferreira, Claudia Cristina Garcia Martin-Didonet
This work aimed to characterize 20 isolates obtained from upland rice plants, based on phenotypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16S rRNA sequencing) and plant growth promotion. Results showed a great morphological, metabolic and genetic variability among bacterial isolates. All isolates showed positive activity for catalase and protease enzymes and, 90% of the isolates showed positive activity for amylase, catalase and, nitrogenase...
August 5, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28827029/temporal-assessment-of-microbial-communities-in-soils-of-two-contrasting-mangroves
#16
Janaina Rigonato, Angela D Kent, Thiago Gumiere, Luiz Henrique Zanini Branco, Fernando Dini Andreote, Marli Fátima Fiore
Variations in microbial communities promoted by alterations in environmental conditions are reflected in similarities/differences both at taxonomic and functional levels. Here we used a natural gradient within mangroves from seashore to upland, to contrast the natural variability in bacteria, cyanobacteria and diazotroph assemblages in a pristine area compared to an oil polluted area along a timespan of three years, based on ARISA (bacteria and cyanobacteria) and nifH T-RFLP (diazotrophs) fingerprinting. The data presented herein indicated that changes in all the communities evaluated were mainly driven by the temporal effect in the contaminated area, while local effects were dominant on the pristine mangrove...
August 1, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28818332/antimicrobial-activity-and-acetylcholinesterase-inhibition-by-extracts-from-chromatin-modulated-fungi
#17
Matheus Thomaz Nogueira Silva Lima, Larissa Batista Dos Santos, Rafael Wesley Bastos, Jacques Robert Nicoli, Jacqueline Aparecida Takahashi
Major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of Alzheimer's disease have led to searching new control drugs. The present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. For such finality, five filamentous fungal species (Talaromyces funiculosus, Talaromyces islandicus, Talaromyces minioluteus, Talaromyces pinophilus, Penicillium janthinellum) were grown or not with DNA methyltransferases inhibitors (procainamide or hydralazine) and/or a histone deacetylase inhibitor (suberohydroxamic acid)...
August 1, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28807610/genome-sequencing-of-two-bacillus-anthracis-strains-a-virulent-strain-and-a-vaccinal-strain
#18
Franciele Maboni Siqueira, Samuel Paulo Cibulski, Fabiana Quoos Mayer, David Driemeier, Saulo Petinatti Pavarini, Agueda Palmira Castagna de Vargas
Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn, the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Both genomes have an overall C+G content of 35.4%, and 11 genes encoding the ribosomal RNAs (rRNAs) 5S, 16S and 23S...
July 31, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28807609/cspb-of-an-arctic-bacterium-polaribacter-irgensii-kopri-22228-confers-extraordinary-freeze-tolerance
#19
Youn Hong Jung, Yoo Kyung Lee, Hong Kum Lee, Kyunghee Lee, Hana Im
Freezing temperatures are a major challenge for life at the poles. Decreased membrane fluidity, uninvited secondary structure formation in nucleic acids, and protein cold-denaturation all occur at cold temperatures. Organisms adapted to polar regions possess distinct mechanisms that enable them to survive in extremely cold environments. Among the cold-induced proteins, cold shock protein (Csp) family proteins are the most prominent. A gene coding for a Csp-family protein, cspB, was cloned from an arctic bacterium, Polaribacter irgensii KOPRI 22228, and overexpression of cspB greatly increased the freeze-survival rates of Escherichia coli hosts, to a greater level than any previously reported Csp...
July 31, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28778371/draft-genome-sequence-of-vitellibacter-aquimaris-d-24-t-isolated-from-seawater
#20
Suganthi Thevarajoo, Chitra Selvaratnam, Kok-Gan Chan, Kian Mau Goh, Chun Shiong Chong
Vitellibacter aquimaris D-24(T) (=KCTC 42708(T)=DSM 101732(T)), a halophilic marine bacterium, was isolated from seawater collected from Desaru beach, Malaysia. Here, we present the draft genome sequence of D-24(T) with a genome size of approximately 3.1Mbp and G+C content of 39.93%. The genome of D-24(T) contains genes involved in reducing a potent greenhouse gas (N2O) in the environment and the degradation of proteinaceous compounds. Genome availability will provide insights into potential biotechnological and environmental applications of this bacterium...
July 19, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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