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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/29866608/petroleum-contamination-and-bioaugmentation-in-bacterial-rhizosphere-communities-from-avicennia-schaueriana
#1
Simone Dealtry, Angela Michelato Ghizelini, Leda C S Mendonça-Hagler, Ricardo Moreira Chaloub, Fernanda Reinert, Tácio M P de Campos, Newton C M Gomes, Kornelia Smalla
Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition...
June 1, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29866609/development-of-an-indirect-elisa-based-on-recombinant-capsid-protein-to-detect-antibodies-to-bovine-leukemia-virus
#2
Ana Paula Andreolla, Luana Marina Scheer Erpen, Rafael Frandoloso, Luiz Carlos Kreutz
Serological testing and culling infected animals are key management practices aiming eradication of bovine leukemia virus infection. Here, we report the development of an indirect ELISA based on BLV recombinant capsid protein (BLVp24r) to detect anti-BLV antibodies in cattle serum. The BLVp24r was expressed in Escherichia coli and purified by affinity chromatography, and then used to set up the ELISA parameters. The Polysorp® plate coated with 50ng of antigen/well and bovine serum diluted 1:100 gave the best results during standardization...
May 22, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29937264/ammonia-determines-transcriptional-profile-of-microorganisms-in-anaerobic-digestion
#3
Nan Zhang, Huijuan Peng, Yong Li, Wenxiu Yang, Yuneng Zou, Huiguo Duan
Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscriptomic analysis, the transcriptional profile of microbial community in anaerobic digestion under low (1500mgL-1 ) and high NH4 + (5000mgL-1 ) concentrations, respectively, were revealed. The results showed that high NH4 + concentrations significantly inhibited methane production but facilitated the accumulations of volatile fatty acids...
May 21, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29891415/shigatoxigenic-and-atypical-enteropathogenic-escherichia-coli-in-fish-for-human-consumption
#4
Marita Vedovelli Cardozo, Clarissa Araújo Borges, Lívia Gerbasi Beraldo, Renato Pariz Maluta, Andressa Souza Pollo, Mariana Monezi Borzi, Luis Fernando Dos Santos, Subhashinie Kariyawasam, Fernando Antônio de Ávila
Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.
May 21, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29858139/detection-of-virulence-and-%C3%AE-lactamase-encoding-genes-in-enterobacter-aerogenes-and-enterobacter-cloacae-clinical-isolates-from-brazil
#5
Paola Aparecida Alves Azevedo, João Pedro Rueda Furlan, Mariana Oliveira-Silva, Rafael Nakamura-Silva, Carolina Nogueira Gomes, Karen Regina Carim Costa, Eliana Guedes Stehling, André Pitondo-Silva
Enterobacter cloacae and E. aerogenes have been increasingly reported as important opportunistic pathogens. In this study, a high prevalence of multi-drug resistant isolates from Brazil, harboring several β-lactamase encoding genes was found. Several virulence genes were observed in E. aerogenes, contrasting with the E. cloacae isolates which presented none.
May 21, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29898867/differential-proteomics-research-of-bacillus-amyloliquefaciens-and-its-genome-shuffled-saltant-for-improving-fengycin-production
#6
Junfeng Zhao, Chong Zhang, Zhaoxin Lu
In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-2-4. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this study, comparative proteomic analysis of the parental ES-2-4 and genome-shuffled FMB72 strains was conducted to examine the differentially expressed proteins. In the shuffled strain FMB72, 50 differently expressed spots (p<0...
May 19, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29858140/carbon-metabolism-influenced-for-promoters-and-temperature-used-in-the-heterologous-protein-production-using-pichia-pastoris-yeast
#7
REVIEW
Andrea B Zepeda, Adalberto Pessoa, Jorge G Farías
Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole carbon and energy source. Pichia pastoris is a methylotrophic yeast characterized as being an economical, fast and effective system for heterologous protein expression. Many factors can affect both the product and the production, including the promoter, carbon source, pH, production volume, temperature, and many others; but to control all of them most of the time is difficult and this depends on the initial selection of each variable...
May 19, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29805073/polycyclic-aromatic-hydrocarbons-degradation-by-marine-derived-basidiomycetes-optimization-of-the-degradation-process
#8
Gabriela A L Vieira, Mariana Juventina Magrini, Rafaella C Bonugli-Santos, Marili V N Rodrigues, Lara D Sette
Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Therefore, this study aimed to select one PAHs degrader among three marine-derived basidiomycete fungi and to study its pyrene detoxification/degradation. Marasmiellus sp. CBMAI 1062 showed higher levels of pyrene and BaP degradation and was subjected to studies related to pyrene degradation optimization using experimental design, acute toxicity, organic carbon removal (TOC), and metabolite evaluation...
May 3, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29784602/molecular-methods-for-diversity-assessment-among-xanthomonads-of-bulgarian-and-macedonian-pepper
#9
Taca Vancheva, Mariya Stoyanova, Elena Tasheva-Terzieva, Nevena Bogatzevska, Penka Moncheva
Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization of Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 strains were identified as Xanthomonas euvesicatoria and 30 as Xanthomonas vesicatoria using species-specific primers and polymerase chain reaction followed by restriction fragment length polymorphism analysis. To assess the genetic diversity of the strains, two methods (Random Amplified Polymorphic DNA and Repetitive Element Palindromic-Polymerase Chain Reaction) were applied...
May 3, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29747929/dynamics-and-diversity-of-the-bacterial-community-during-the-spontaneous-decay-of-a%C3%A3-ai-euterpe-oleracea-fruits
#10
Fábio Gomes Moura, Diego Assis das Graças, Agenor Valadares Santos, Artur Luiz da Costa da Silva, Hervé Rogez
The biodiversity and evolution of the microbial community in açai fruits (AF) between three geographical origins and two spontaneous decay conditions were examined by applying culture-independent methods. Culture-independent methods based on 16S rRNA from fifteen samples revealed that Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Acidobacteria were the most abundant phyla. At the genus level, Massilia (taxon with more than 50% of the sequences remaining constant during the 30h of decay), Pantoea, Naxibacter, Enterobacter, Raoultella and Klebsiella were identified, forming the carposphere bacterial microbiota of AF...
April 30, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29752010/comparison-of-genexpert-mtb-rif-assay-and-led-fm-microscopy-for-the-diagnosis-of-extra-pulmonary-tuberculosis-in-khyber-pakhtunkhwa-pakistan
#11
Anwar Sheed Khan, Sajid Ali, Muhammad Tahir Khan, Sajjad Ahmed, Yasir Khattak, Abduljabbar, Muhammad Irfan, Wasim Sajjad
GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n=390) to 47% (n=347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy...
April 27, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29728338/carotenoid-production-in-sugarcane-juice-and-synthetic-media-supplemented-with-nutrients-by-rhodotorula-rubra-l02
#12
Mariana de Paula Bonadio, Lidyane Aline de Freita, Márcia Justino Rossini Mutton
In order for the use of biological carotenoids to become feasible, it is necessary to have adequate low cost sources and improved methods of cultivation. The aim of this study was to evaluate the effect of supplementation with nitrogen, phosphorus, zinc, and magnesium, on the biomass and carotenoid volumetric production by yeast Rhodotorula rubra L02 using a complex medium (sugarcane juice) and synthetic media (sucrose and maltose) as substrates. The experimental design used for each substrate was randomized in blocks with 16 treatments and 3 repetitions...
April 25, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29773508/comparative-proteomic-analyses-of-hyphozyma-roseonigra-atcc-20624-in-response-to-sclareol
#13
Xiuwen Wang, Xiaohua Zhang, Qingshou Yao, Dongliang Hua, Jiayang Qin
Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29739645/plant-growth-promoting-rhizobacteria-reduce-aphid-population-and-enhance-the-productivity-of-bread-wheat
#14
Muhammad Naeem, Zubair Aslam, Abdul Khaliq, Jam Nazir Ahmed, Ahmad Nawaz, Mubshar Hussain
Plant growth promoting rhizobacteria increase plant growth and give protection against insect pests and pathogens. Due to the negative impact of chemical pesticides on environment, alternatives to these chemicals are needed. In this scenario, the biological methods of pest control offer an eco-friendly and an attractive option. In this study, the effect of two plant growth promoting rhizobacterial strains (Bacillus sp. strain 6 and Pseudomonas sp. strain 6K) on aphid population and wheat productivity was evaluated in an aphid susceptible (Pasban-90) and resistant (Inqlab-91) wheat cultivar...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29728342/statistical-optimization-of-kojic-acid-production-by-a-uv-induced-mutant-strain-of-aspergillus-terreus
#15
Mojtaba Shakibaie, Atefeh Ameri, Roya Ghazanfarian, Mahboubeh Adeli-Sardou, Sahar Amirpour-Rostami, Masoud Torkzadeh-Mahani, Mehdi Imani, Hamid Forootanfar
The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5-40min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10 , 7.63g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KH2 PO4 (NH4 )2 SO4 , and pH) influences on kojic acid production by the C5-10 mutant...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29728341/adopt-a-bacterium-an-active-and-collaborative-learning-experience-in-microbiology-based-on-social-media
#16
Marco Aurélio Floriano Piantola, Ana Carolina Ramos Moreno, Heloísa Alonso Matielo, Natalia Pasternak Taschner, Rafael Ciro Marques Cavalcante, Samia Khan, Rita de Cássia Café Ferreira
The "Adopt a Bacterium" project is based on the use of social network as a tool in Microbiology undergraduate education, improving student learning and encouraging students to participate in collaborative learning. The approach involves active participation of both students and teachers, emphasizing knowledge exchange, based on widely used social media. Students were organized in groups and asked to adopt a specific bacterial genus and, subsequently, submit posts about "adopted genus". The formative assessment is based on posting information on Facebook®, and the summative assessment involves presentation of seminars about the adopted theme...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29728340/combined-application-of-bio-organic-phosphate-and-phosphorus-solubilizing-bacteria-bacillus-strain-mwt-14-improve-the-performance-of-bread-wheat-with-low-fertilizer-input-under-an-arid-climate
#17
Muhammad Tahir, Umaira Khalid, Muhammad Ijaz, Ghulam Mustafa Shah, Muhammad Asif Naeem, Muhammad Shahid, Khalid Mahmood, Naveed Ahmad, Fazal Kareem
This study was aimed to investigate the effect of bio-organic phosphate either alone or in combination with phosphorus solubilizing bacteria strain (Bacillus MWT-14) on the growth and productivity of two wheat cultivars (Galaxy-2013 and Punjab-2011) along with recommended (150-100NPkgha-1 ) and half dose (75-50NPkgha-1 ) of fertilizers. The combined application of bio-organic phosphate and the phosphorous solubilizing bacteria strain at either fertilizer level significantly improved the growth, yield parameters and productivity of both wheat cultivars compared to non-inoculated control treatments...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29728339/first-report-of-the-production-of-a-potent-biosurfactant-with-%C3%AE-%C3%AE-trehalose-by-fusarium-fujikuroi-under-optimized-conditions-of-submerged-fermentation
#18
Cristiane Bianchi Loureiro Dos Reis, Liziane Maria Barassuol Morandini, Caroline Borges Bevilacqua, Fabricio Bublitz, Gustavo Ugalde, Marcio Antonio Mazutti, Rodrigo Josemar Seminoti Jacques
Biosurfactants have many advantages over synthetic surfactants but have higher production costs. Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost. The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule. The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29720352/bacteremia-and-meningitis-caused-by-oxa-23-producing-acinetobacter-baumannii-molecular-characterization-and-susceptibility-testing-for-alternative-antibiotics
#19
Kamile Francine Schuertz, Felipe Francisco Tuon, Jussara Kasuko Palmeiro, Danieli Conte, João Paulo Marochi Telles, Lucas Eduardo Trevisoli, Libera Maria Dalla-Costa
BACKGROUND: Carbapenem-resistant Acinetobacter baumannii infection is a concern in developing countries due to high incidence, few therapeutic options, and increasing costs. OBJECTIVE: Characterize and analyze the antibiotic susceptibility patterns of carbapenem-resistant A. baumannii isolates and evaluate clinical data of meningitis and bacteremia caused by this microorganism. METHODS: Twenty-six A. baumannii isolates from 23 patients were identified by MALDI-TOF and automated methods and genotyped using pulsed field genotyping electrophoresis...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29720351/a-high-prevalence-of-human-papillomavirus-16-and-18-co-infections-in-cervical-biopsies-from-southern-brazil
#20
Sheile Pinheiro de Jesus, Ana Carla Marques da Costa, Regina Bones Barcellos, Rubia Marília de Medeiros, Cláudia Maria Dornelles da Silva, Maria Lucia Rossetti
HPV types 16 and 18 were studied in paraffin-fixed cervical biopsy collected in southern Brazil. HPV 16, HPV 18 and co-infection HPV 16/18 were identified in 10/57 (17.5%), 4/57 (7%) and in 43/57 (75.4%) samples, respectively. Southern Brazil has one of the highest prevalence rates of HPV 16/18 reported.
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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