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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/30181051/the-use-of-prevotella-bryantii-3c5-for-modulation-of-the-ruminal-environment-in-an-ovine-model
#1
Martín Fraga, Sofía Fernández, Karen Perelmuter, Nicolle Pomiés, Cecilia Cajarville, Pablo Zunino
In the Southern Hemisphere, ruminants are mostly raised in grazing systems where animals consume forage and are supplemented with low amounts of concentrates. Concentrates are usually given separately and are rapidly ingested. This practice leads to changing rumen environment conditions during the day, may alter the rumen microbial metabolism and could affect host performance. The native ruminal Prevotella bryantii strain 3C5 was administered every 48h to wethers under experimental conditions simulating Southern-Hemisphere feeding to evaluate its potential as a rumen fermentation modulator...
August 25, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30181050/antimicrobial-effect-of-copper-surfaces-on-bacteria-isolated-from-poultry-meat
#2
Angel Parra, Magaly Toro, Ricardo Jacob, Paola Navarrete, Miriam Troncoso, Guillermo Figueroa, Angélica Reyes-Jara
Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens - Salmonella enterica and Listeria monocytogenes - and a poultry native microbiota bacterial species - Enterobacter cloacae...
August 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30170962/survey-on-pathogenic-escherichia-coli-and-salmonella-spp-in-captive-cockatiels-nymphicus-hollandicus
#3
Patricia Silveira de Pontes, Selene Dall' Acqua Coutinho, Renata de Oliveira Iovine, Marcos Paulo Vieira Cunha, Terezinha Knöbl, Vania Maria de Carvalho
We surveyed healthy captive cockatiels (Nymphicus hollandicus) for Escherichia coli and Salmonella spp. Cloacal swabs were collected from 94 cockatiels kept in commercial breeders, private residencies and pet shops in the cities of São Paulo/SP and Niterói/RJ (Brazil). Three strains of E. coli from each individual were tested for the presence of ExPEC-, APEC- and DEC-related genes. We evaluated the blaTEM , blaSHV , blaOXA , blaCMY , blaCTX-M , tetA, tetB, aadA, aphA, strAB, sul1, sul2, sul3, qnrA, qnrD, qnrB, qnrS, oqxAB, aac (6)'-Ib-cr, qepA resistance genes and markers for plasmid incompatibility groups...
August 20, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30166270/bioconversion-of-waste-cooking-oil-glycerol-from-cabbage-extract-to-lactic-acid-by-rhizopus-microsporus
#4
Thalisa Yuwa-Amornpitak, Kannika Chookietwatana
Glycerol from spent oil was processed by transesterification for biodiesel production. Although glycerol contains many types of impurities, it can be used as a C-source for lactic acid production by fungi, such as Rhizopus microsporus. In this study, we found that wild type R. microsporus (LTH23) produced more lactic acid than the mutant strains on cabbage glycerol media (CG media). More lactic acid was produced on CG media than on cabbage extract media (C media) by about two-fold in batch fermentation conditions...
August 18, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30166269/rio-negro-virophage-sequencing-of-the-near-complete-genome-and-transmission-electron-microscopy-of-viral-factories-and-particles
#5
Iara Apolinário Borges, Felipe Lopes de Assis, Ludmila Karen Dos Santos Silva, Jônatas Abrahão
Rio Negro virophage (RNV) was co-isolated with a strain of mimivirus named sambavirus, from Brazilian Amazon. We report the near complete genome sequence of RNV, the first virophage isolated in Brazil. We also present new microscopical data demonstrating that RNV particles have similar dimensions to that described to sputnik virophages.
August 17, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30166267/accuracy-of-heparin-binding-protein-as-a-new-marker-in-prediction-of-acute-bacterial-meningitis
#6
Mona Kandil, Gihane Khalil, Eman El-Attar, Gihan Shehata, Salwa Hassan
BACKGROUND: Cerebrospinal fluid bacterial culture is the gold-standard for confirmation of acute bacterial meningitis, but many cases are not culture confirmed. Antibiotics reduce the chance of a microbiological diagnosis. Objective to evaluate efficacy of Heparin-binding protein in diagnosis of bacterial meningitis. PATIENTS: 30 patients diagnosed with acute bacterial meningitis, 30 viral meningitis, and 30 subjects with normal CSF findings. DESIGN: Diagnosis was based on history, clinical criteria, CSF examination, latex agglutination & culture, and sensitivities and response to therapy...
August 17, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30166266/diversity-and-communities-of-culturable-endophytic-fungi-from-different-tree-peonies-geoherbs-and-non-geoherbs-and-their-biosynthetic-potential-analysis
#7
Guodong Yang, Peng Li, Lifen Meng, Keyi Xv, Faming Dong, Yan Qiu, Lei He, Lin Lin
To know more about the potential roles of endophytic fungi in the formation mechanism of Daodi medicinal material, diversity and communities of culturable endophytic fungi in three types of tree peonies were investigated. Endophytic fungi of three types of tree peonies were isolated and identified. The diversity was analyzed. Bayesian trees constructed by MrBayes 3.2.6 after phylogenetic analysis of the ITS sequences. The endophytic fungi potential for synthesis of natural products was assessed by means of detecting NRPS and PKS gene sequences...
August 17, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30166268/evaluation-of-skimmed-milk-flocculation-method-for-virus-recovery-from-tomatoes
#8
Fabiana Gil Melgaço, Adriana Abreu Corrêa, Ana Carolina Ganime, Marcelo Luiz Lima Brandão, Valéria de Mello Medeiros, Carla de Oliveira Rosas, Silvia Maria Dos Reis Lopes, Marize Pereira Miagostovich
This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV)...
August 16, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30150085/characterization-of-klebsiella-pneumoniae-associated-with-cattle-infections-in-southwest-china-using-multi-locus-sequence-typing-mlst-antibiotic-resistance-and-virulence-associated-gene-profile-analysis
#9
Fangjun Cheng, Zhangcheng Li, Shimei Lan, Wei Liu, Xiaoyan Li, Zuoyong Zhou, Zhenhui Song, Juan Wu, Manli Zhang, Wenjie Shan
Klebsiella pneumoniae is important human and animal pathogen that causes a wide spectrum of infections. In this study, isolates from cattle nasal swabs samples were identified by 16S rRNA, and to evaluate the antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing of K. pneumoniae isolates. 33 isolates of K. pneumoniae were isolated and identified in 213 nasal swabs samples, of which 12 were hypervirulent K. pneumoniae strains. Extended Spectrum Beta-Lactamases genes were found in 93...
August 16, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30150087/endophytic-bacillus-strains-enhance-pearl-millet-growth-and-nutrient-uptake-under-low-p
#10
Vitória Palhares Ribeiro, Ivanildo Evódio Marriel, Sylvia Morais de Sousa, Ubiraci Gomes de Paula Lana, Bianca Braz Mattos, Christiane Abreu de Oliveira, Eliane Aparecida Gomes
Bacterial endophytes are considered to have a beneficial effect on host plants, improving their growth by different mechanisms. The objective of this study was to investigate the capacity of four endophytic Bacillus strains to solubilize iron phosphate (Fe-P), produce siderophores and indole-acetic acid (IAA) in vitro, and to evaluate their plant growth promotion ability in greenhouse conditions by inoculation into pearl millet cultivated in a P-deficient soils without P fertilization, with Araxá rock phosphate or soluble triple superphosphate...
August 14, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30150086/antimicrobial-potential-of-pyroligneous-extracts-a-systematic-review-and-technological-prospecting
#11
REVIEW
Juliana Leitzke Santos de Souza, Victoria Burmann da Silva Guimarães, Angela Diniz Campos, Rafael Guerra Lund
Pyroligneous extract is applied in diverse areas as an antioxidant, an antimicrobial, and an anti-inflammatory agent. The discovery of new cost-effective antimicrobial agents of natural origin remains a challenge for the scientific community. This study aimed to conduct a systematic review and a technological forecasting of the existent evidence regarding the use of pyroligneous extract as a potential antimicrobial agent. Studies were identified through an investigation of various electronic databases: PubMed, SciFinder, Web of Science, Scopus, Scielo, Google scholar, and ProQuest...
August 14, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30146181/non-viral-microbial-keratitis-in-adults-clinical-and-laboratory-aspects
#12
Eunice Stella Jardim Cury, Marilene Rodrigues Chang, Elenir Rose Jardim Cury Pontes
This study compares patients with and without non-viral microbial keratitis in relation to sociodemographic variables, clinical aspects, and involved causative agent. Clinical aspects, etiology and therapeutic procedures were assessed in patients with and without keratitis that were diagnosed in an Eye Care Center in Campo Grande, MS, Brazil. Patients were divided into two groups: (a) cases: 64 patients with non-viral microbial keratitis diagnosed at biomicroscopy; and (b) controls: 47 patients with other eye disorders that were not keratitis...
August 14, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30249525/duplex-nested-pcr-for-detection-of-small-ruminant-lentiviruses
#13
Rebeca C Marinho, Gabrielle R Martins, Kelma C Souza, Ana Lídia M Sousa, Sabrina Tainah C Silva, Juliana A Nobre, Maria F S Teixeira
Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosis of SRLV. The technique was designed and tested with the control strains CAEV Co and MVV 1514. Then, field strains were submitted to the same protocol of duplex nested-PCR. Blood samples of sheep and goats were tested with AGID and nested PCR with specific primers for pol, gag and LTR...
August 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30145264/pleurotus-spp-cultivation-on-brachiaria-sp-straw-treatment-with-alkaline-water-oyster-mushroom-and-substrate-treatment
#14
Matheus Rodrigo Iossi, Juan Diego Valenzuela Cobos, Francisco J Gea Alegria, Diego Cunha Zied
The aim of this research was to evaluate the efficiency of aqueous alkali-treated Brachiaria straw for the cultivation of appropriate species of oyster mushroom. The substrate used in the cultivation of various Pleurotus spp. was soaked for 20min by using two different procedures: (i) 0.5-2.0% Ca(OH)2 in 100L water, and (ii) 50-250L water. As a result, 1% Ca(OH)2 dissolved in 100L water and 3.5kg of Brachiaria straw presented the best production. The most suitable species for the application of the present method were P...
August 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30145263/influence-of-iron-and-copper-on-the-activity-of-laccases-in-fusarium-oxysporum-f-sp-lycopersici
#15
Wendy Susana Hernández-Monjaraz, César Caudillo-Pérez, Pedro Ulises Salazar-Sánchez, Karla Lizbeth Macías-Sánchez
Fusarium oxysporum f. sp. lycopersici is a phytopathogenic fungus that causes vascular wilt in tomato plants. In this work we analyze the influence of metal salts such as iron and copper sulphate, as well as that of bathophenanthrolinedisulfonic acid (iron chelator) and bathocuproinedisulfonic acid (copper chelator) on the activity of laccases in the intra (IF) and extracellular fractions (EF) of the wild-type and the non-pathogenic mutant strain (rho1::hyg) of F. oxysporum. The results show that laccase activity in the IF fraction of the wild and mutant strain increased with the addition of iron chelator (53...
August 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30174203/screening-of-endophytic-fungi-stored-in-a-culture-collection-for-taxol-production
#16
Hortência Farias de Andrade, Lívia Caroline Alexandre de Araújo, Bruno Souza Dos Santos, Patrícia Maria Guedes Paiva, Thiago Henrique Napoleão, Maria Tereza Dos Santos Correia, Maria Betânia Melo de Oliveira, Gláucia Manoella de Souza Lima, Rafael Matos Ximenes, Túlio Diego da Silva, Girliane Regina da Silva, Márcia Vanusa da Silva
In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection 'University Recife Mycologia' (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.
August 11, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30170963/characterization-of-avian-pathogenic-escherichia-coli-isolated-from-free-range-helmeted-guineafowl
#17
Mariana Monezi Borzi, Marita Vedovelli Cardozo, Elisabete Schirato de Oliveira, Andressa de Souza Pollo, Elisabete Aparecida Lopes Guastalli, Luis Fernando Dos Santos, Fernando Antonio de Ávila
Avian pathogenic Escherichia coli (APEC) isolates from apparently healthy free range helmeted guineafowl were characterized. Most of them had a high frequency of virulence associated genes, multi drug resistance and high pathogenicity. We demonstrated that helmeted guineafowl have potential to transmit antibiotic resistant APEC to other species including humans.
August 11, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30177270/direct-identification-of-bovine-mastitis-pathogens-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-in-pre-incubated-milk
#18
Juliana R Barreiro, Juliano L Gonçalves, Rafaella Grenfell, Renata F Leite, Luiz Juliano, Marcos V Santos
The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were screened based on culture-positive results obtained by microbiological culture (reference method) with the following numbers of quarters positive per cow: 4 cows with 1, 8 cows with 2, 12 cows with 3 and 16 cows with 4 infected quarters per cow...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30174202/the-occurrence-of-aflatoxigenic-aspergillus-spp-in-dairy-cattle-feed-in-southern-brazil
#19
Augusto Carlos Favaretto Variane, Fabiane Cristina Dos Santos, Fausto Fernandes de Castro, Ione Parra Barbosa-Tessmann, Geraldo Tadeu Dos Santos, Magali Soares Dos Santos Pozza
The presence of mycotoxins or related fungi in animal feed is a major problem for animal and human health. Silage and concentrated feed samples were collected from 21 dairy farms in the Western part of Paraná state in Southern Brazil. Water activity and pH of all samples were measured, and each sample was analyzed to check for the presence of aflatoxigenic Aspergillus. Water activity was observed to be lower in the concentrated feed samples. The pH was lower in the silage samples, indicating fermentation processes...
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/30150084/multi-enzyme-complex-of-white-rot-fungi-in-saccharification-of-lignocellulosic-material
#20
Wilton Soares Cardoso, Paula Viana Queiroz, Gabriella Peterlini Tavares, Fernando Almeida Santos, Filippe Elias de Freitas Soares, Maria Catarina Megumi Kasuya, José Humberto de Queiroz
The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.
October 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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