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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/28633981/development-of-a-propidium-monoazide-polymerase-chain-reaction-assay-for-detection-of-viable-lactobacillus-brevis-in-beer
#1
Yanlin Ma, Yang Deng, Zhenbo Xu, Junyan Liu, Jianjun Dong, Hua Yin, Junhong Yu, Zongming Chang, Dongfeng Wang
The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this paper, the combination of propidium monoazide (PMA) pretreatment and conventional PCR had been described. The established PMA-PCR identified beer spoilage Lactobacillus brevis based not on their identity, but on the presence of horA gene which we show to be highly correlated with the ability of beer spoilage LAB to grow in beer...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629972/characterization-of-dioxygenases-and-biosurfactants-produced-by-crude-oil-degrading-soil-bacteria
#2
Santhakumar Muthukamalam, Sivalingam Sivagangavathi, Dharmapal Dhrishya, Sadras Sudha Rani
Role of microbes in bioremediation of oil spills has become inevitable owing to their eco friendly nature. This study focused on the isolation and characterization of bacterial strains with superior oil degrading potential from crude-oil contaminated soil. Three such bacterial strains were selected and subsequently identified by 16S rRNA gene sequence analysis as Corynebacterium aurimucosum, Acinetobacter baumannii and Microbacterium hydrocarbonoxydans respectively. The specific activity of catechol 1,2 dioxygenase (C12O) and catechol 2,3 dioxygenase (C23O) was determined in these three strains wherein the activity of C12O was more than that of C23O...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629971/survey-of-pathogens-in-threatened-wild-red-tailed-amazon-parrot-amazona-brasiliensis-nestlings-in-rasa-island-brazil
#3
Frederico Fontanelli Vaz, Patrícia Pereira Serafini, Rosangela Locatelli-Dittrich, Rafael Meurer, Edison Luiz Durigon, Jansen de Araújo, Luciano Matsumiya Thomazelli, Tatiana Ometto, Elenise Angelotti Bastos Sipinski, Rafael Meirelles Sezerban, Maria Cecília Abbud, Tânia Freitas Raso
The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629969/prevalence-and-serotype-distribution-of-listeria-monocytogenes-isolated-from-foods-in-montevideo-uruguay
#4
Valeria Braga, Sylvia Vázquez, Victoria Vico, Valeria Pastorino, María Inés Mota, Marcela Legnani, Felipe Schelotto, Gustavo Lancibidad, Gustavo Varela
The aim of this work was to study the prevalence of Listeria monocytogenes in foods obtained in retail shops and food industries located in Montevideo-Uruguay, and to identify the serogroups of the obtained isolates. Three-thousand one-hundred and seventy-five food samples (frozen, deli meats, ready-to-eat and cheese) were analyzed. The obtained isolates were serogrouped by multiplex PCR and serotyped by conventional procedure. Genetic comparisons were performed using pulsed-field gel electrophoresis on a sub-set of isolates belonging to the same serotype successively recovered from the same establishment...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629968/inhibitor-tolerance-of-a-recombinant-flocculating-industrial-saccharomyces-cerevisiae-strain-during-glucose-and-xylose-co-fermentation
#5
Yun-Cheng Li, Zi-Xi Gou, Ying Zhang, Zi-Yuan Xia, Yue-Qin Tang, Kenji Kida
Lignocellulose-derived inhibitors have negative effects on the ethanol fermentation capacity of Saccharomyces cerevisiae. In this study, the effects of eight typical inhibitors, including weak acids, furans, and phenols, on glucose and xylose co-fermentation of the recombinant xylose-fermenting flocculating industrial S. cerevisiae strain NAPX37 were evaluated by batch fermentation. Inhibition on glucose fermentation, not that on xylose fermentation, correlated with delayed cell growth. The weak acids and the phenols showed additive effects...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629967/isolation-of-fungi-from-dung-of-wild-herbivores-for-application-in-bioethanol-production
#6
Rhulani Makhuvele, Ignatious Ncube, Elbert Lukas Jansen van Rensburg, Daniël Coenrad La Grange
Producing biofuels such as ethanol from non-food plant material has the potential to meet transportation fuel requirements in many African countries without impacting directly on food security. The current shortcomings in biomass processing are inefficient fermentation of plant sugars, such as xylose, especially at high temperatures, lack of fermenting microbes that are able to resist inhibitors associated with pre-treated plant material and lack of effective lignocellulolytic enzymes for complete hydrolysis of plant polysaccharides...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28625768/screening-and-characterization-of-lactic-acid-bacterial-strains-that-produce-fermented-milk-and-reduce-cholesterol-levels
#7
Xuefang Guan, Qingxian Xu, Yi Zheng, Lei Qian, Bin Lin
OBJECTIVE: To screen for and characterize lactic acid bacteria strains with the ability to produce fermented milk and reduce cholesterol levels. METHODS: The strains were isolated from traditional fermented milk in China. In vitro and in vivo evaluation of cholesterol-reduction were used to identify and verify strains of interest. Characteristics were analyzed using spectrophotometry and plate counting assays. RESULTS: The isolate HLX37 consistently produced fermented milk with strong cholesterol-reducing properties was identified as Lactobacillus plantarum (accession number: KR105940) and was thus selected for further study...
June 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28619663/captive-wild-birds-as-reservoirs-of-enteropathogenic-e-coli-epec-and-shiga-toxin-producing-e-coli-stec
#8
Lilian Aparecida Sanches, Marcelo da Silva Gomes, Rodrigo Hidalgo Friciello Teixeira, Marcos Paulo Vieira Cunha, Maria Gabriela Xavier de Oliveira, Mônica Aparecida Midolli Vieira, Tânia Aparecida Tardelli Gomes, Terezinha Knobl
Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of São Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes...
June 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28610831/complete-genome-sequence-of-the-aerobically-denitrifying-thermophilic-bacterium-chelatococcus-daeguensis-tad1
#9
Yunlong Yang, Ershu Lin, Shaobin Huang
Chelatococcus daeguensis TAD1 is a themophilic bacterium isolated from a biotrickling filter used to treat NOx in Ruiming Power Plant, located in Guangzhou, China, which shows an excellent aerobic denitrification activity at high temperature. The complete genome sequence of this strain was reported in the present study. Genes related to the aerobic denitrification were identified through whole genome analysis. This work will facilitate the mechanism of aerobic denitrification and provide evidence for its potential application in the nitrogen removal...
June 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28606427/illumina-based-analysis-of-endophytic-bacterial-diversity-of-tree-peony-paeonia-sect-moutan-roots-and-leaves
#10
Ruixian Yang, Ping Liu, Wenyu Ye
Diverse communities of bacteria inhabit plant tissues and those bacteria play a crucial role for plant health and growth. Tree peony (Paeonia Sect. Moutan) is known for its excellent ornamental and medicinal values as Chinese traditional plant, but little is known about its associated bacterial community under natural conditions. To examine how endophytic bacteria in tree peony vary across tissues and cultivars, PCR-based Illumina was applied to reveal the diversity of endophytic bacteria in tree peony. A total of 149,842 sequences and 21,463 operational taxonomic units (OTUs) were obtained...
June 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629970/variation-analysis-of-bacterial-polyhydroxyalkanoates-production-using-saturated-and-unsaturated-hydrocarbons
#11
Saiqa Tufail, Sajida Munir, Nazia Jamil
Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping...
May 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28623104/zearalenone-adsorption-capacity-of-lactic-acid-bacteria-isolated-from-pigs
#12
María F Vega, Susana N Dieguez, Belén Riccio, Sandra Aranguren, Antonio Giordano, Laura Denzoin, Alejandro L Soraci, María O Tapia, Romina Ross, Ana Apás, Silvia N González
The ability to adsorb zearalenone by five strain of lactic acid bacteria was evaluated: four strains of Lactobacillus spp. isolated from pig rectal swabs and one commercial strain (Lactobacillus rhamnosus). Several factors affecting the adsorption capacity were evaluated in order to improve the adsorption of the mycotoxin by bacteria. The stability of the zearalenone-bacteria complex was analyzed. In every case, bacterial adsorption capacity was higher than 40.0%. The strain showing the highest adsorption (68...
May 27, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28552660/technology-and-safety-assessment-for-lactic-acid-bacteria-isolated-from-traditional-bulgarian-fermented-meat-product-lukanka
#13
Svetoslav Dimitrov Todorov, Saso Stojanovski, Ilia Iliev, Penka Moncheva, Luis Augusto Nero, Iskra Vitanova Ivanova
The present work discusses the technological and new selection criteria that should be included for selecting lactic acid bacteria for production of fermented meat. Lactic acid bacteria isolated from Bulgarian traditional fermented "lulanka" salami was studied regarding some positive technological parameters (growth at different temperature, pH, and proteolytic activity). The presence of genes related to the virulence factors, production of biogenic amines, and vancomycin resistance were presented in low frequency in the studied lactic acid bacteria...
May 11, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28552659/antimicrobial-activity-evaluation-and-comparison-of-methods-of-susceptibility-for-klebsiella-pneumoniae-carbapenemase-kpc-producing-enterobacter-spp-isolates
#14
Daniele Zendrini Rechenchoski, Angélica Marim Lopes Dambrozio, Ana Carolina Polano Vivan, Paulo Alfonso Schuroff, Tatiane das Neves Burgos, Marsileni Pelisson, Marcia Regina Eches Perugini, Eliana Carolina Vespero
The production of KPC (Klebsiella pneumoniae carbapenemase) is the major mechanism of resistance to carbapenem agents in enterobacterias. In this context, forty KPC-producing Enterobacter spp. clinical isolates were studied. It was evaluated the activity of antimicrobial agents: polymyxin B, tigecycline, ertapenem, imipenem and meropenem, and was performed a comparison of the methodologies used to determine the susceptibility: broth microdilution, Etest(®) (bioMérieux), Vitek 2(®) automated system (bioMérieux) and disc diffusion...
May 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28410799/genomic-identification-and-characterization-of-the-elite-strains-bradyrhizobium-yuanmingense-br-3267-and-bradyrhizobium-pachyrhizi-br-3262-recommended-for-cowpea-inoculation-in-brazil
#15
Jakson Leite, Samuel Ribeiro Passos, Jean Luiz Simões-Araújo, Norma Gouvêa Rumjanek, Gustavo Ribeiro Xavier, Jerri Édson Zilli
The leguminous inoculation with nodule-inducing bacteria that perform biological nitrogen fixation is a good example of an "eco-friendly agricultural practice". Bradyrhizobium strains BR 3267 and BR 3262 are recommended for cowpea (Vigna unguiculata) inoculation in Brazil and showed remarkable responses; nevertheless neither strain was characterized at species level, which is our goal in the present work using a polyphasic approach. The strains presented the typical phenotype of Bradyrhizobium with a slow growth and a white colony on yeast extract-mannitol medium...
March 31, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28256389/analysis-of-microbial-diversity-in-shenqu-with-different-fermentation-times-by-pcr-dgge
#16
Tengfei Liu, Tianzhu Jia, Jiangning Chen, Xiaoyu Liu, Minjie Zhao, Pengpeng Liu
Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods. DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of Pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and that the predominant fungi were uncultured Rhizopus, Aspergillus oryzae, and Rhizopus oryzae...
April 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28162954/production-of-docosahexaenoic-acid-by-aurantiochytrium-sp-atcc-pra-276
#17
Valcenir Júnior Mendes Furlan, Victor Maus, Irineu Batista, Narcisa Maria Bandarra
The high costs and environmental concerns associated with using marine resources as sources of oils rich in polyunsaturated fatty acids have prompted searches for alternative sources of such oils. Some microorganisms, among them members of the genus Aurantiochytrium, can synthesize large amounts of these biocompounds. However, various parameters that affect the polyunsaturated fatty acids production of these organisms, such as the carbon and nitrogen sources supplied during their cultivation, require further elucidation...
April 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28130020/helicobacter-pylori-with-east-asian-type-cagpai-genes-is-more-virulent-than-strains-with-western-type-in-some-cagpai-genes
#18
Xiao-Yan Yuan, Jin-Jun Yan, Ya-Chao Yang, Chun-Mei Wu, Yan Hu, Jian-Li Geng
The severity of Helicobacter pylori-related disease is correlated with the presence and integrity of a cag pathogenicity island (cagPAI). cagPAI genotype may have a modifying effect on the pathogenic potential of the infecting strain. After analyzing the sequences of cagPAI genes, some strains with the East Asian-type cagPAI genes were selected for further analysis to examine the association between the diversity of the cagPAI genes and the virulence of H. pylori. The results showed that gastric mucosal inflammatory cell infiltration was significantly higher in patients with East Asian-type cagPAI genes H...
April 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28110805/predictive-modeling-of-pseudomonas-fluorescens-growth-under-different-temperature-and-ph-values
#19
Letícia Dias Dos Anjos Gonçalves, Roberta Hilsdorf Piccoli, Alexandre de Paula Peres, André Vital Saúde
Meat is one of the most perishable foods owing to its nutrient availability, high water activity, and pH around 5.6. These properties are highly conducive for microbial growth. Fresh meat, when exposed to oxygen, is subjected to the action of aerobic psychrotrophic, proteolytic, and lipolytic spoilage microorganisms, such as Pseudomonas spp. The spoilage results in the appearance of slime and off-flavor in food. In order to predict the growth of Pseudomonas fluorescens in fresh meat at different pH values, stored under refrigeration, and temperature abuse, microbial mathematical modeling was applied...
April 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28108350/molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-blood-in-rio-de-janeiro-displaying-susceptibility-profiles-to-non-%C3%AE-lactam-antibiotics
#20
Alexandra Vidal Pedinotti Zuma, Danielle Ferreira Lima, Ana Paula D'Alincourt Carvalho Assef, Elizabeth Andrade Marques, Robson Souza Leão
The distinction between healthcare-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA) infections has become increasingly blurred. We assessed the molecular characterization and antimicrobial resistance profile for MRSA isolates from blood. Most of all (81.9%) isolates are related to known HA-MRSA and CA-MRSA epidemic lineages, such as, USA300, USA400, USA600, USA800 and USA1100. This is the first multicenter study in Rio de Janeiro.
April 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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