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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/27899238/goat-umbilical-cord-cells-are-permissive-to-small-ruminant-lentivirus-infection-in-vitro
#1
Gabrielle R Martins, Rebeca C Marinho, Rosivaldo Q Bezerra Junior, Antoniel de O Alves, Lilia M C Câmara, Luiz C Albuquerque-Pinto, Maria F da S Teixeira
Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture...
November 19, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27889420/correlation-of-phenotypic-tests-with-the-presence-of-the-blaz-gene-for-detection-of-beta-lactamase
#2
Adriano Martison Ferreira, Katheryne Benini Martins, Vanessa Rocha da Silva, Alessandro Lia Mondelli, Maria de Lourdes Ribeiro de Souza da Cunha
Staphylococcus aureus and Staphylococcus saprophyticus are the most common and most important staphylococcal species associated with urinary tract infections. The objective of the present study was to compare and to evaluate the accuracy of four phenotypic methods for the detection of beta-lactamase production in Staphylococcus spp. Seventy-three strains produced a halo with a diameter ≤28mm (penicillin resistant) and all of them were positive for the blaZ gene. Among the 28 susceptible strain (halo ≥29mm), 23 carried the blaZ gene and five did not...
November 11, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27889421/production-of-native-arbuscular-mycorrhizal-fungi-inoculum-under-different-environmental-conditions
#3
Yamir Torres-Arias, Rosalba Ortega Fors, Camila Nobre, Eduardo Furrazola Gómez, Ricardo Luis Louro Berbara
In order to obtain an arbuscular mycorrhizal fungi (AMF) native inoculum from Sierra de Moa and determine the most appropriate conditions for its big scale production, four light and temperature combinations were tested in three plant species (Calophyllum antillanum, Talipariti elatum and Paspalum notatum). Growth and development parameters, as well as the mycorrhizal functioning of the seedlings were evaluated. The natural light treatment under high temperatures (L-H) was the most suitable for the growth and development of the three plant species, showing the highest total biomass values, mainly of root, and a positive root-shoot ratio balance...
November 8, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27876549/nitrogen-fixing-bacteria-and-arbuscular-mycorrhizal-fungi-in-piptadenia-gonoacantha-mart-macbr
#4
Joel Quintino de Oliveira Júnior, Ederson da Conceição Jesus, Francy Junio Lisboa, Ricardo Luis Louro Berbara, Sergio Miana de Faria
The family Leguminosae comprises approximately 20,000 species that mostly form symbioses with arbuscular mycorrhizal fungi (AMF) and nitrogen-fixing bacteria (NFB). This study is aimed at investigating and confirming the dependence on nodulation and biological nitrogen fixation in the specie Piptadenia gonoacantha (Mart.) Macbr., which belongs to the Piptadenia group. Two consecutive experiments were performed in a greenhouse. The experiments were fully randomized with six replicates and a factorial scheme...
November 8, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27865632/detection-of-mycobacterium-avium-subsp-paratuberculosis-in-bovine-milk-from-the-state-of-pernambuco-brazil
#5
Pedro Paulo Feitosa de Albuquerque, André de Souza Santos, Orestes Luiz de Souza Neto, Pomy de Cássia Peixoto Kim, Erika Fernanda Torres Samico Fernandes Cavalcanti, Júnior Mário Baltazar de Oliveira, Rinaldo Aparecido Mota, José Wilton Pinheiro Júnior
The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied...
November 8, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27866935/diarrheagenic-escherichia-coli
#6
REVIEW
Tânia A T Gomes, Waldir P Elias, Isabel C A Scaletsky, Beatriz E C Guth, Juliana F Rodrigues, Roxane M F Piazza, Luís C S Ferreira, Marina B Martinez
Most Escherichia coli strains live harmlessly in the intestines and rarely cause disease in healthy individuals. Nonetheless, a number of pathogenic strains can cause diarrhea or extraintestinal diseases both in healthy and immunocompromised individuals. Diarrheal illnesses are a severe public health problem and a major cause of morbidity and mortality in infants and young children, especially in developing countries. E. coli strains that cause diarrhea have evolved by acquiring, through horizontal gene transfer, a particular set of characteristics that have successfully persisted in the host...
November 5, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27818093/genome-sequence-of-bradyrhizobium-embrapense-strain-cnpso-2833-t-isolated-from-a-root-nodule-of-desmodium-heterocarpon
#7
Jakeline Renata Marçon Delamuta, Renan Augusto Ribeiro, Douglas Fabiano Gomes, Renata Carolini Souza, Ligia Maria Oliveira Chueire, Mariangela Hungria
Bradyrhizobium embrapense CNPSo 2833(T) is a nitrogen-fixing symbiont of the legume pasture Desmodium. Its draft genome contains 8,267,832bp and 7876 CDSs. The symbiotic island includes nodulation and nitrogen fixation genes resembling the operon organization of B. japonicum. Several CDSs related to secretion proteins and stress tolerance were also identified.
November 3, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27865633/celebration-of-60th-anniversary-of-brazilian-society-for-microbiology
#8
EDITORIAL
Marina B Martinez, Carlos P Taborda
No abstract text is available yet for this article.
November 2, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27866936/current-applications-and-different-approaches-for-microbial-l-asparaginase-production
#9
REVIEW
Jorge Javier Muso Cachumba, Felipe Antonio Fernandes Antunes, Guilherme Fernando Dias Peres, Larissa Pereira Brumano, Júlio César Dos Santos, Silvio Silvério Da Silva
l-asparaginase (EC 3.5.1.1) is an enzyme that catalysis mainly the asparagine hydrolysis in l-aspartic acid and ammonium. This enzyme is presented in different organisms, such as microorganisms, vegetal, and some animals, including certain rodent's serum, but not unveiled in humans. It can be used as important chemotherapeutic agent for the treatment of a variety of lymphoproliferative disorders and lymphomas (particularly acute lymphoblastic leukemia (ALL) and Hodgkin's lymphoma), and has been a pivotal agent in chemotherapy protocols from around 30 years...
October 27, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27825766/microbiology-of-organic-and-conventionally-grown-fresh-produce
#10
REVIEW
Daniele F Maffei, Erika Y Batalha, Mariza Landgraf, Donald W Schaffner, Bernadette D G M Franco
Fresh produce is a generalized term for a group of farm-produced crops, including fruits and vegetables. Organic agriculture has been on the rise and attracting the attention of the food production sector, since it uses eco-agricultural principles that are ostensibly environmentally-friendly and provides products potentially free from the residues of agrochemicals. Organic farming practices such as the use of animal manure can however increase the risk of contamination by enteric pathogenic microorganisms and may consequently pose health risks...
October 27, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27818091/mosquito-transmitted-viruses-the-great-brazilian-challenge
#11
REVIEW
Mânlio Tasso de Oliveira Mota, Ana Carolina Terzian, Maria Luana Cristiny Rodrigues Silva, Cássia Estofolete, Maurício Lacerda Nogueira
Arboviruses pose a serious threat to public health worldwide, overloading the healthcare system and causing economic losses. These viruses form a very diverse group, and in Brazil, arboviruses belonging to the families Flaviviridae and Togaviridae are predominant. Unfortunately, the number of arboviruses increases in proportion with factors such as deforestation, poor sanitation, climate changes, and introduction of new viruses like Chikungunya virus and Zika virus. In Brazil, dengue is endemic, along with the presence of other arboviruses...
October 27, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27838289/biopharmaceuticals-from-microorganisms-from-production-to-purification
#12
REVIEW
Angela Faustino Jozala, Danilo Costa Geraldes, Louise Lacalendola Tundisi, Valker de Araújo Feitosa, Carlos Alexandre Breyer, Samuel Leite Cardoso, Priscila Gava Mazzola, Laura de Oliveira-Nascimento, Carlota de Oliveira Rangel-Yagui, Pérola de Oliveira Magalhães, Marcos Antonio de Oliveira, Adalberto Pessoa
The use of biopharmaceuticals dates from the 19th century and within 5-10 years, up to 50% of all drugs in development will be biopharmaceuticals. In the 1980s, the biopharmaceutical industry experienced a significant growth in the production and approval of recombinant proteins such as interferons (IFN α, β, and γ) and growth hormones. The production of biopharmaceuticals, known as bioprocess, involves a wide range of techniques. In this review, we discuss the technology involved in the bioprocess and describe the available strategies and main advances in microbial fermentation and purification process to obtain biopharmaceuticals...
October 26, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27825606/microbial-interactions-ecology-in-a-molecular-perspective
#13
REVIEW
Raíssa Mesquita Braga, Manuella Nóbrega Dourado, Welington Luiz Araújo
The microorganism-microorganism or microorganism-host interactions are the key strategy to colonize and establish in a variety of different environments. These interactions involve all ecological aspects, including physiochemical changes, metabolite exchange, metabolite conversion, signaling, chemotaxis and genetic exchange resulting in genotype selection. In addition, the establishment in the environment depends on the species diversity, since high functional redundancy in the microbial community increases the competitive ability of the community, decreasing the possibility of an invader to establish in this environment...
October 26, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27825605/antimicrobial-resistance-in-enterobacteriaceae-in-brazil-focus-on-%C3%AE-lactams-and-polymyxins
#14
REVIEW
Jorge Luiz Mello Sampaio, Ana Cristina Gales
During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96...
October 25, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27818090/ethanol-production-in-brazil-a-bridge-between-science-and-industry
#15
REVIEW
Mario Lucio Lopes, Silene Cristina de Lima Paulillo, Alexandre Godoy, Rudimar Antonio Cherubin, Marcel Salmeron Lorenzi, Fernando Henrique Carvalho Giometti, Claudemir Domingues Bernardino, Henrique Berbert de Amorim Neto, Henrique Vianna de Amorim
In the last 40 years, several scientific and technological advances in microbiology of the fermentation have greatly contributed to evolution of the ethanol industry in Brazil. These contributions have increased our view and comprehension about fermentations in the first and, more recently, second-generation ethanol. Nowadays, new technologies are available to produce ethanol from sugarcane, corn and other feedstocks, reducing the off-season period. Better control of fermentation conditions can reduce the stress conditions for yeast cells and contamination by bacteria and wild yeasts...
October 25, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27913076/biofilm-production-and-beta-lactamic-resistance-in-brazilian-staphylococcus-aureus-isolates-from-bovine-mastitis
#16
Viviane Figueira Marques, Cássia Couto da Motta, Bianca da Silva Soares, Dayanne Araújo de Melo, Shana de Mattos de Oliveira Coelho, Irene da Silva Coelho, Helene Santos Barbosa, Miliane Moreira Soares de Souza
Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation...
October 18, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27789202/use-of-green-fluorescent-protein-labeled-non-tuberculous-mycobacteria-to-evaluate-the-activity-quaternary-ammonium-compound-disinfectants-and-antibiotics
#17
Claudia Cortesia, Teresita Bello, Gustavo Lopez, Scott Franzblau, Jacobus de Waard, Howard Takiff
Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recently been many non-tuberculous mycobacteria soft tissue infections after minor surgical procedures, some apparently related to the use of a commercial disinfectant based on a Quaternary Ammonium Compound. We studied the activity of this and other quaternary ammonium compounds on different non-tuberculous mycobacteria by transforming the mycobacteria with a dnaA-gfp fusion and then monitoring fluorescence to gauge the capacity of different quaternary ammonium compounds to inhibit bacterial growth...
October 11, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27777013/potential-for-biocontrol-of-melanized-fungi-by-actinobacteria-isolated-from-intertidal-region-of-ilha-do-mel-paran%C3%A3-brazil
#18
Camila de Araújo Dalitz, Mariana Vieira Porsani, Izabel Cristina Figel, Ida C Pimentel, Patrícia R Dalzoto
Actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. Identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16S region of ribosomal DNA (rDNA), which is conserved and present in all bacteria, is an important method of identification. Melanized fungi are free-living organisms, which can also be pathogens of clinical importance. This work aimed to evaluate growth inhibition of melanized fungi by actinobacteria and to identify the latter to the species level...
October 11, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27780663/characterization-of-vibrio-cholerae-isolates-from-1976-to-2013-in-shandong-province-china
#19
Hui Lü, Yuqi Yuan, Na Sun, Zhenwang Bi, Bing Guan, Kun Shao, Tongzhan Wang, Zhenqiang Bi
Cholera continues to be a serious public health issue in developing countries. We analyzed the epidemiological data of cholera from 1976 to 2013 in Shandong Province, an eastern coastal area of China. A total of 250 Vibrio cholerae isolates were selected for PCR analysis of virulence genes and pulsed-field gel electrophoresis (PFGE). The analysis of the virulence genes showed that the positive rates for tcpA and tcpI were the highest among strains from the southwest region, which had the highest incidence rate of cholera...
October 10, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27793541/comparison-of-culture-and-pcr-methods-in-the-diagnosis-of-bacterial-meningitis
#20
Emel Ödemiş Başpınar, Saim Dayan, Muhammed Bekçibaşı, Recep Tekin, Celal Ayaz, Özcan Deveci, Salih Hoşoğlu
Our aim in this study is to compare the standard culture method with the multiplex PCR and the Speed-Oligo(®) Bacterial Meningitis Test (SO-BMT) - a hybridization-based molecular test method - during the CSF examination of the patients with the pre-diagnosis of acute bacterial meningitis. For the purposes of this study, patients with acute bacterial meningitis treated at the Dicle University Medical Faculty Hospital, Infectious Diseases and Clinical Microbiology Clinic between December 2009 and April 2012 were retrospectively evaluated...
October 6, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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