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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/28807610/genome-sequencing-of-two-bacillus-anthracis-strains-a-virulent-strain-and-a-vaccinal-strain
#1
Franciele Maboni Siqueira, Samuel Paulo Cibulski, Fabiana Quoos Mayer, David Driemeier, Saulo Petinatti Pavarini, Agueda Palmira Castagna de Vargas
Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn, the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Both genomes have an overall C+G content of 35.4%, and 11 genes encoding the ribosomal RNAs (rRNAs) 5S, 16S and 23S...
July 31, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28807609/cspb-of-an-arctic-bacterium-polaribacter-irgensii-kopri-22228-confers-extraordinary-freeze-tolerance
#2
Youn Hong Jung, Yoo Kyung Lee, Hong Kum Lee, Kyunghee Lee, Hana Im
Freezing temperatures are a major challenge for life at the poles. Decreased membrane fluidity, uninvited secondary structure formation in nucleic acids, and protein cold-denaturation all occur at cold temperatures. Organisms adapted to polar regions possess distinct mechanisms that enable them to survive in extremely cold environments. Among the cold-induced proteins, cold shock protein (Csp) family proteins are the most prominent. A gene coding for a Csp-family protein, cspB, was cloned from an arctic bacterium, Polaribacter irgensii KOPRI 22228, and overexpression of cspB greatly increased the freeze-survival rates of Escherichia coli hosts, to a greater level than any previously reported Csp...
July 31, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28778371/draft-genome-sequence-of-vitellibacter-aquimaris-d-24-t-isolated-from-seawater
#3
Suganthi Thevarajoo, Chitra Selvaratnam, Kok-Gan Chan, Kian Mau Goh, Chun Shiong Chong
Vitellibacter aquimaris D-24(T) (=KCTC 42708(T)=DSM 101732(T)), a halophilic marine bacterium, was isolated from seawater collected from Desaru beach, Malaysia. Here, we present the draft genome sequence of D-24(T) with a genome size of approximately 3.1Mbp and G+C content of 39.93%. The genome of D-24(T) contains genes involved in reducing a potent greenhouse gas (N2O) in the environment and the degradation of proteinaceous compounds. Genome availability will provide insights into potential biotechnological and environmental applications of this bacterium...
July 19, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28774638/mangrove-endophyte-promotes-reforestation-tree-acacia-polyphylla-growth
#4
Renata Assis Castro, Manuella Nóbrega Dourado, Jaqueline Raquel de Almeida, Paulo Teixeira Lacava, André Nave, Itamar Soares de Melo, João Lucio de Azevedo, Maria Carolina Quecine
Mangroves are ecosystems located in the transition zone between land and sea that serve as a potential source of biotechnological resources. Brazil's extensive coast contains one of the largest mangrove forests in the world (encompassing an area of 25,000km(2) along all the coast). Endophytic bacteria were isolated from the following three plant species: Rhizophora mangle, Laguncularia racemosa and Avicennia nitida. A large number of these isolates, 115 in total, were evaluated for their ability to fix nitrogen and solubilize phosphorous...
July 19, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28774637/complete-genome-sequence-of-human-pathogen-kosakonia-cowanii-type-strain-888-76-t
#5
Xue-Jing Yang, Sai Wang, Jun-Min Cao, Jia-Hui Hou
Kosakonia cowanii type strain 888-76(T) is a human pathogen which was originally isolated from blood as NIH group 42. In this study, we report the complete genome sequence of K. cowanii 888-76(T). 888-76(T) has 1 chromosome and 2 plasmids with a total genome size of 4,857,567bp and C+G 56.15%. This genome sequence will not only help us to understand the virulence features of K. cowanii 888-76(T) but also provide us the useful information for the study of evolution of Kosakonia genus.
July 19, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28774639/draft-genome-sequence-of-chryseobacterium-limigenitum-sur2-t-lmg-28734-t-isolated-from-dehydrated-sludge
#6
Jure Škraban, Nikos C Kyrpides, Nicole Shapiro, William B Whitman, Janja Trček
The type strain SUR2 of the novel species Chryseobacterium limigenitum was isolated from a dehydrated sludge of the municipal sewage treatment plant in Dogoše near Maribor in Slovenia. The draft genome, with 60 contigs, 4,697,725bp, 34.4% of G+C content, was obtained using the Illumina HiSeq 2500-1 platform. Joint Genome Institute Microbial Genome Annotation Pipeline (MGAP v.4) has identified 4322 protein-coding sequences including resistance genes against arsenic and other heavy metals. In addition, a subclass B3 metallo-β-lactamase, which confers resistance to penicillins, cephalosporins and carbapenems, was also present in the genome...
July 18, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28757098/draft-genome-sequence-of-exiguobacterium-aurantiacum-strain-pn47-isolate-from-saline-ponds-known-as-salar-del-huasco-located-in-the-altiplano-in-the-north-of-chile
#7
Erwin Strahsburger, Felipe Zapata, Inti Pedroso, Derie Fuentes, Paz Tapia, Raul Ponce, Jorge Valdes
In this report, we present a draft genome of 2,886,173bp of an Exiguobacterium aurantiacum strain PN47 isolate from the sediment of a saline pond named "Salar del Huasco" in the Altiplano in the North of Chile. Strain PN47 encodes adaptive characteristics enabling survival in extreme environmental conditions of high heavy metal and salt concentrations and high alkalinity.
July 18, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28754299/antimicrobial-resistance-in-neisseria-gonorrhoeae-history-molecular-mechanisms-and-epidemiological-aspects-of-an-emerging-global-threat
#8
REVIEW
Ana Paula Ramalho da Costa Lourenço, Késia Thaís Barros Dos Santos, Beatriz Meurer Moreira, Sergio Eduardo Longo Fracalanzza, Raquel Regina Bonelli
Neisseria gonorrhoeae is the agent of gonorrhea, a sexually transmitted infection with an estimate from The World Health Organization of 78 million new cases in people aged 15-49 worldwide during 2012. If left untreated, complications may include pelvic inflammatory disease and infertility. Antimicrobial treatment is usually effective; however, resistance has emerged successively through various molecular mechanisms for all the regularly used therapeutic agents throughout decades. Detection of antimicrobial susceptibility is currently the most critical aspect for N...
July 12, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28735852/roles-of-quorum-sensing-molecules-from-rhizobium-etli-rt1-in-bacterial-motility-and-biofilm-formation
#9
Swarnita Dixit, Ramesh Chand Dubey, Dinesh Kumar Maheshwari, Prahlad Kishore Seth, Vivek K Bajpai
Strain RT1 was isolated from root nodules of Lens culinaris (a lentil) and characterized as Rhizobium etli (a Gram-negative soil-borne bacterium) by 16S rDNA sequencing and phylogenetic analysis. The signaling molecules produced by R. etli (RT1) were detected and identified by high-performance liquid chromatography coupled with mass spectrometry. The most abundant and biologically active N-acyl homoserine lactone molecules (3-oxo-C8-HSL and 3-OH-C14-HSL) were detected in the ethyl acetate extract of RT1. The biological role of 3-oxo-C8-HSL was evaluated in RT1...
July 8, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28739413/production-of-recombinant-flagellin-to-develop-elisa-based-detection-of-salmonella-enteritidis
#10
Seyed Ali Mirhosseini, Abbas Ali Imani Fooladi, Jafar Amani, Hamid Sedighian
Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen...
July 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28720432/adhesion-and-invasion-of-clostridium-perfringens-type-a-into-epithelial-cells
#11
Luis A Llanco, Viviane Nakano, Claudia T P de Moraes, Roxane M F Piazza, Mario J Avila-Campos
Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated...
July 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28756029/tripartite-symbiosis-of-sophora-tomentosa-rhizobia-and-arbuscular-mycorhizal-fungi
#12
Maíra Akemi Toma, Teotonio Soares de Carvalho, Amanda Azarias Guimarães, Elaine Martins da Costa, Jacqueline Savana da Silva, Fatima Maria de Souza Moreira
Sophora tomentosa is a pantropical legume species with potential for recovery of areas degraded by salinization, and for stabilization of sand dunes. However, few studies on this species have been carried out, and none regarding its symbiotic relationship with beneficial soil microorganisms. Therefore, this study aimed to evaluate the diversity of nitrogen-fixing bacteria isolated from nodules of Sophora tomentosa, and to analyze the occurrence of colonization of arbuscular mycorrhizal fungi on the roots of this legume in seafront soil...
June 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28689814/isolation-and-characterization-of-a-novel-endo-%C3%AE-1-4-glucanase-from-a-metagenomic-library-of-the-black-goat-rumen
#13
Yun-Hee Song, Kyung-Tai Lee, Jin-Young Baek, Min-Ju Kim, Mi-Ra Kwon, Young-Joo Kim, Mi-Rim Park, Haesu Ko, Jin-Sung Lee, Keun-Sung Kim
The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized...
June 26, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28716388/identification-of-co-infection-by-rotavirus-and-parvovirus-in-dogs-with-gastroenteritis-in-mexico
#14
Ariadna Flores Ortega, José Simón Martínez-Castañeda, Linda G Bautista-Gómez, Raúl Fajardo Muñoz, Israel Quijano Hernández
This is the first report on circulating canine rotavirus in Mexico. Fifty samples from dogs with gastroenteritis were analyzed used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were infected with rotavirus exclusively, while 14% were co-infected with both rotavirus and parvovirus; clinical signs in co-infected dogs were more severe.
June 24, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28689813/combination-of-commercially-available-molecular-assays-and-culture-based-methods-in-diagnosis-of-tuberculosis-and-drug-resistant-tuberculosis
#15
Lamprini Gkaravela, Matthaios Papadimitriou-Olivgeris, Antigoni Foka, Fevronia Kolonitsiou, Anastasia Spiliopoulou, Nikolaos Charokopos, Apostolos Voulgaridis, Maria Tsiamita, Markos Marangos, Evangelos D Anastassiou, Iris Spiliopoulou
Early diagnosis of tuberculosis is of major clinical importance. Among 4733 clinical specimens collected from 3363 patients and subjected to Ziehl-Neelsen microscopy, 4109 were inoculated onto Löwenstein-Jensen slants and 3139 in Bactec/9000MB. Polymerase Chain Reaction (PCR) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 Mycobacterium tuberculosis complex PCR-positive for isoniazid and rifampicin resistance detection specimens (GenoType MTBDRplus). Recovered M. tuberculosis isolates (64) as well as, 21 more sent from Regional Hospitals were tested for antimycobacterial resistance with a phenotypic (manual MGIT-SIRE) and a genotypic assay (GenoType MTBDRplus)...
June 24, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28651890/isolation-and-molecular-characterization-of-thraustochytrium-strain-isolated-from-antarctic-peninsula-and-its-biotechnological-potential-in-the-production-of-fatty-acids
#16
Esteban Caamaño, Lyliam Loperena, Ivonne Hinzpeter, Paulina Pradel, Felipe Gordillo, Gino Corsini, Mario Tello, Paris Lavín, Alex R González
Thraustochytrids are unicellular protists belonging to the Labyrinthulomycetes class, which are characterized by the presence of a high lipid content that could replace conventional fatty acids. They show a wide geographic distribution, however their diversity in the Antarctic Region is rather scarce. The analysis based on the complete sequence of 18S rRNA gene showed that strain 34-2 belongs to the species Thraustochytrium kinnei, with 99% identity. The total lipid profile shows a wide range of saturated fatty acids with abundance of palmitic acid (16:0), showing a range of 16...
June 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28645649/evaluation-of-a-selective-chromogenic-medium-for-detecting-vancomycin-resistant-enterococci
#17
Renata Oliveira Soares, Adriana Medianeira Rossato, Gustavo Enck Sambrano, Neidimar Cezar Corrêa Tolfo, Juliana Caierão, Thiago Galvão da Silva Paim, Pedro Alves d'Azevedo
Rapid identification of vancomycin-resistant enterococci (VRE) can assist in choosing the appropriate treatment and preventing VRE spread. The performance of chromID™ VRE agar was evaluated using 184 clinical isolates of Enterococcus spp. and reference strains. The test had a sensitivity of 95.52% but a low specificity of 30%.
June 8, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28648636/contribution-of-flagella-and-motility-to-gut-colonisation-and-pathogenicity-of-salmonella-enteritidis-in-the-chicken
#18
Fernanda de Oliveira Barbosa, Oliveiro Caetano de Freitas Neto, Diego Felipe Alves Batista, Adriana Maria de Almeida, Marcela da Silva Rubio, Lucas Bocchini Rodrigues Alves, Rosemeire de Oliveira Vasconcelos, Paul Andrew Barrow, Angelo Berchieri Junior
Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes...
June 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28645648/promotion-of-iron-nutrition-and-growth-on-peanut-by-paenibacillus-illinoisensis-and-bacillus-sp-strains-in-calcareous-soil
#19
Di Liu, Qianqian Yang, Ke Ge, Xiuna Hu, Guozhen Qi, Binghai Du, Kai Liu, Yanqin Ding
This study aimed to explore the effects of two siderophore-producing bacterial strains on iron absorption and plant growth of peanut in calcareous soil. Two siderophore-producing bacterial strains, namely, YZ29 and DZ13, isolated from the rhizosphere soil of peanut, were identified as Paenibacillus illinoisensis and Bacillus sp., respectively. In potted experiments, YZ29 and DZ13 enhanced root activity, chlorophyll and active iron content in leaves, total nitrogen, phosphorus and potassium accumulation of plants and increased the quality of peanut kernels and plant biomass over control...
June 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28651889/a-novel-expression-vector-for-the-secretion-of-abaecin-in-bacillus-subtilis
#20
Li Li, Lan Mu, Xiaojuan Wang, Jingfeng Yu, Ruiping Hu, Zhen Li
This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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