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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/29249565/comparative-study-of-rflp-is6110-and-miru-vntr-from-mycobacterium-tuberculosis-isolated-in-the-state-of-minas-gerais-brazil
#1
Cláudio José Augusto, Wânia da Silva Carvalho, Isabela Neves de Almeida, Lida Jouca de Assis Figueiredo, Nayanne Gama Teixeira Dantas, Philip Noel Suffys, Silvana Spíndola de Miranda
DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compare the similarity, discriminatory power, and agreement of the clusters between the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci Variable Number Tandem Repeat - Mycobacterial Interspersed Repetitive Units (MIRU-VNTR) techniques...
December 11, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29246664/phenolic-content-and-antibacterial-activity-of-extracts-of-hamelia-patens-obtained-by-different-extraction-methods
#2
Jorge Enrique Wong Paz, Carolina Rubio Contreras, Abigail Reyes Munguía, Cristóbal Noé Aguilar, María Luisa Carrillo Inungaray
Hamelia patens, is a plant traditionally used to treat a variety of conditions among the Huastec people of Mexico. The objective of this study is to characterize the phenolic content and critically examine the antimicrobial activity of leaf extracts H. patens, obtained by maceration, Soxhlet and percolation, using ethanol as 70% solvent. Phenolic compounds are characterized by liquid chromatography, coupled to a High Resolution Mass Spectrometry, and the antimicrobial activity was studied from the inhibitory effect of each extract for Escherichia coli, Staphylococcus aureus, Salmonella typhi and S...
December 6, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29233483/development-and-validation-of-a-modified-taqman-based-real-time-pcr-assay-targeting-the-lipl32-gene-for-detection-of-pathogenic-leptospira-in-canine-urine-samples
#3
Bruno Alonso Miotto, Aline Santana da Hora, Sueli Akemi Taniwaki, Paulo Eduardo Brandão, Marcos Bryan Heinemann, Mitika Kuribayashi Hagiwara
A modified TaqMan real-time polymerase chain reaction targeting a 138bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA using the TaqMan-based assay. The results were compared with those from a well-established conventional PCR targeting the 16S RNA encoding gene associated with nucleotide sequencing analysis...
December 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29229530/trichoderma-harzianum-mtcc-5179-impacts-the-population-and-functional-dynamics-of-microbial-community-in-the-rhizosphere-of-black-pepper-piper-nigrum-l
#4
Palaniyandi Umadevi, Muthuswamy Anandaraj, Vivek Srivastav, Sailas Benjamin
Employing Illumina Hiseq whole genome metagenome sequencing approach, we studied the impact of Trichoderma harzianum on altering the microbial community and its functional dynamics in the rhizhosphere soil of black pepper (Piper nigrum L.). The metagenomic datasets from the rhizosphere with (treatment) and without (control) T. harzianum inoculation were annotated using dual approach, i.e., stand alone and MG-RAST. The probiotic application of T. harzianum in the rhizhosphere soil of black pepper impacted the population dynamics of rhizosphere bacteria, archae, eukaryote as reflected through the selective recruitment of bacteria [Acidobacteriaceae bacterium (p=1...
November 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29254630/arbuscular-mycorrhizal-fungal-communities-in-the-rhizosphere-of-a-continuous-cropping-soybean-system-at-the-seedling-stage
#5
Jiaqi Cui, Li Bai, Xiaorui Liu, Weiguang Jie, Baiyan Cai
Arbuscular mycorrhizae (AM) fungi play a crucial role in the growth of soybean; however, the planting system employed is thought to have an effect on AM fungal communities in the rhizosphere. This study was performed to explore the influence of continuous soybean cropping on the diversity of Arbuscular mycorrhizal (AM) fungi, and to identify the dominant AM fungus during the seedling stage. Three soybean cultivars were planted under two and three years continuous cropping, respectively. The diversity of AM fungi in the rhizosphere soil at the seedling stage was subsequently analyzed using polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE)...
November 14, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29229529/evaluation-of-root-knot-nematode-disease-control-and-plant-growth-promotion-potential-of-biofertilizer-ning-shield-on-trichosanthes-kirilowii-in-the-field
#6
Chun-Hao Jiang, Ping Xie, Ke Li, Yue-Sheng Xie, Liu-Jun Chen, Jin-Suo Wang, Quan Xu, Jian-Hua Guo
Biofertilizer Ning shield was composed of different strains of plant growth promotion bacteria. In this study, the plant growth promotion and root-knot nematode disease control potential on Trichosanthes kirilowii in the field were evaluated. The application of Ning shield significantly reduced the diseases severity caused by Meloidogyne incognita, the biocontrol efficacy could reached up to 51.08%. Ning shield could also promote the growth of T. kirilowii in the field by increasing seedling emergence, height and the root weight...
November 11, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29132828/lactoferrin-chitosan-and-melaleuca-alternifolia-natural-products-that-show-promise-in-candidiasis-treatment
#7
REVIEW
Lorena de Oliveira Felipe, Willer Ferreira da Silva Júnior, Katialaine Corrêa de Araújo, Daniela Leite Fabrino
The evolution of microorganisms resistant to many medicines has become a major challenge for the scientific community around the world. Motivated by the gravity of such a situation, the World Health Organization released a report in 2014 with the aim of providing updated information on this critical scenario. Among the most worrying microorganisms, species from the genus Candida have exhibited a high rate of resistance to antifungal drugs. Therefore, the objective of this review is to show that the use of natural products (extracts or isolated biomolecules), along with conventional antifungal therapy, can be a very promising strategy to overcome microbial multiresistance...
November 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29129408/efficiency-of-decolorization-of-different-dyes-using-fungal-biomass-immobilized-on-different-solid-supports
#8
Wioletta Przyśtas, Ewa Zabłocka-Godlewska, Elżbieta Grabińska-Sota
Different technologies may be used for decolorization of wastewater containing dyes. Among them, biological processes are the most promising because they seem to be environmentally safe. The aim of this study was to determine the efficiency of decolorization of two dyes belonging to different classes (azo and triphenylmethane dyes) by immobilized biomass of strains of fungi (Pleurotus ostreatus - BWPH, Gleophyllum odoratum - DCa and Polyporus picipes - RWP17). Different solid supports were tested for biomass immobilization...
November 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29128395/structure-analysis-of-capsid-protein-of-porcine-circovirus-type-2-from-pigs-with-systemic-disease
#9
Danielle Gava, Vitor Hugo Balasco Serrão, Lana Teixeira Fernandes, Mauricio Egídio Cantão, Janice Reis Ciacci-Zanella, Nelson Morés, Rejane Schaefer
Economic losses with high mortality rate associated with Porcine circovirus type 2 (PCV2) is reported worldwide. PCV2 commercial vaccine was introduced in 2006 in U.S. and in 2008 in Brazil. Although PCV2 vaccines have been widely used, cases of PCV2 systemic disease have been reported in the last years. Eleven nursery or fattening pigs suffering from PCV2 systemic disease were selected from eight PCV2-vaccinated farms with historical records of PCV2 systemic disease in Southern Brazil. PCV2 genomes were amplified and sequenced from lymph node samples of selected pigs...
November 8, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29157901/inducible-cellulase-production-from-an-organic-solvent-tolerant-bacillus-sp-sv1-and-evolutionary-divergence-of-endoglucanase-in-different-species-of-the-genus-bacillus
#10
Lebin Thomas, Hari Ram, Ved Pal Singh
Bacteria are important sources of cellulases with various industrial and biotechnological applications. In view of this, a non-hemolytic bacterial strain, tolerant to various environmental pollutants (heavy metals and organic solvents), showing high cellulolytic index (7.89) was isolated from cattle shed soil and identified as Bacillus sp. SV1 (99.27% pairwise similarity with Bacillus korlensis). Extracellular cellulases showed the presence of endoglucanase, total cellulase and β-glucosidase activities. Cellulase production was induced in presence of cellulose (3...
November 6, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29254631/the-improvement-of-bioactive-secondary-metabolites-accumulation-in-rumex-gmelini-turcz-through-co-culture-with-endophytic-fungi
#11
Chang-Hong Ding, Qian-Bo Wang, Shenglei Guo, Zhen-Yue Wang
Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer...
November 5, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29157900/a-practical-molecular-identification-of-nonfermenting-gram-negative-bacteria-from-cystic-fibrosis
#12
Carolina Paulino da Costa Capizzani, Natália Candido Caçador, Elizabeth Andrade Marques, Carlos Emílio Levy, Ludmilla Tonani, Lidia Alice Gomes Monteiro Marin Torres, Ana Lúcia da Costa Darini
Identification of nonfermenting Gram-negative bacteria (NFGNB) of cystic fibrosis patients is hard and misidentification could affect clinical outcome. This study aimed to propose a scheme using polymerase chain reaction to identify NFGNB. This scheme leads to reliable identification within 3 days in an economically viable manner when compared to other methods.
November 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29154013/the-potential-of-the-newly-isolated-thermotolerant-yeast-pichia-kudriavzevii-rz8-1-for-high-temperature-ethanol-production
#13
Nuttaporn Chamnipa, Sudarat Thanonkeo, Preekamol Klanrit, Pornthap Thanonkeo
High potential, thermotolerant, ethanol-producing yeasts were successfully isolated in this study. Based on molecular identification and phylogenetic analysis, the isolated thermotolerant yeasts were clustered in the genera of Pichia kudriavzevii, Candida tropicalis, Candida orthopsilosis, Candida glabrata and Kodamea ohmeri. A comparative study of ethanol production using 160g/L glucose as a substrate revealed several yeast strains that could produce high ethanol concentrations at high temperatures. When sugarcane bagasse (SCB) hydrolysate containing 85g/L glucose was used as a substrate, the yeast strain designated P...
November 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29157899/epidemiology-of-diabetic-foot-infections-in-a-reference-tertiary-hospital-in-india
#14
Sanjith Saseedharan, Manisa Sahu, Roonam Chaddha, Edwin Pathrose, Arun Bal, Pallavi Bhalekar, Priyadharshini Sekar, Padma Krishnan
INTRODUCTION: The present study attempts to examine the microbial profile and antibiotic susceptibility of diabetic foot infections in the intensive care unit of a tertiary referral centre for diabetic foot. As part of the study, we also attempted to find the prevalence of blaNDM-like gene among carbapenem-resistant gram negative infections. METHODOLOGY: A prospective study of 261 patients with diabetic foot infections was performed during the period between January 2014 and June 2014...
November 1, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29154014/evaluation-of-sample-extracting-methods-of-fcsm-by-lactobacillus-acidophilus-based-on-a-uplc-q-tof-ms-global-metabolomics-analysis
#15
Yongqiang Wang, Wenju Zhang, Cunxi Nie, Cheng Chen, Xiaoyang Zhang, Jianhe Hu
The study of metabolomics requires extracting as many metabolites as possible from a biological sample. This study aimed to determine the optimal method for the extraction of metabolites from solid-state fermented cottonseed meal (FCSM). The UPLC-Q-TOF-MS global metabolomics technology was used to detect the metabolites in FCSM, and the extraction quantity and extraction efficiency of seven different extraction methods, specifically the WA, 50MeOH, 50MeOHB, 50MeCNB, 80MeOHB, 80MeOH and AMF methods were evaluated...
October 31, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29146152/improved-antifungal-activity-of-barley-derived-chitinase-i-gene-that-overexpress-a-32kda-recombinant-chitinase-in-escherichia-coli-host
#16
Nida Toufiq, Bushra Tabassum, Muhammad Umar Bhatti, Anwar Khan, Muhammad Tariq, Naila Shahid, Idrees Ahmad Nasir, Tayyab Husnain
Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I gene was isolated from the genomic DNA of Barley (Hordeum vulgare L.) cultivar, Haider-93. The isolated DNA was used as template for the amplification of the ∼935bp full-length chitinase I gene...
October 31, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29153452/diagnostics-of-tuberculosis-and-differentiation-of-nonspecific-tuberculin-reactions-in-animals
#17
Sovetzhan Z Basybekov, Marat B Bazarbayev, Bolat A Yespembetov, Assiya Mussaeva, Serik G Kanatbayev, Kanapya M Romashev, Aigul K Dossanova, Tokseiit A Yelekeyev, Elmira K Akmatova, Nazym S Syrym
Tuberculosis is a serious disease of humans and animals, caused by bacteria of the Mycobacterium genus. This leads to complications in the life of the sick person, and subsequently to death. The cattle, who have been diagnosed with this bacterium, are usually sent to the slaughter, with the result that their livestock is reduced. Mycobacteriosis is also a disease, after determining which cattle are most often sent to slaughter. Such a reduction in livestock numbers has a negative effect on the economy. Of the 300 samples from the animals, 25 cultures of atypical bacteria responding to tuberculin were isolated...
October 25, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29122479/draft-genome-sequence-of-paraburkholderia-tropica-ppe8-strain-a-sugarcane-endophytic-diazotrophic-bacterium
#18
Paula Renata Alves da Silva, Jean Luiz Simões-Araújo, Márcia Soares Vidal, Leonardo Magalhães Cruz, Emanuel Maltempi de Souza, José Ivo Baldani
Paraburkholderia tropica (syn Burkholderia tropica) are nitrogen-fixing bacteria commonly found in sugarcane. The Paraburkholderia tropica strain Ppe8 is part of the sugarcane inoculant consortium that has a beneficial effect on yield. Here, we report a draft genome sequence of this strain elucidating the mechanisms involved in its interaction mainly with Poaceae. A genome size of approximately 8.75Mb containing 7844 protein coding genes distributed in 526 subsystems was de novo assembled with ABySS and annotated by RAST...
October 18, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29122478/long-term-cryopreservation-of-basidiomycetes
#19
REVIEW
Giani Andrea Linde, Alana Luciani, Ana Daniela Lopes, Juliana Silveira do Valle, Nelson Barros Colauto
Basidiomycetes have several biotechnological and industrial applications such as enzyme production, bioremediation, pharmaceutical and functional food production. Due to climatic features, the preservation of several basidiomycetes is threatened, and to guarantee the preservation of this genetic resource, the development of long-term preservation techniques is necessary once there is no universal protocol for the cryopreservation of basidiomycetes. Cryopreservation is a technique in which microorganisms are submitted to ultralow temperatures...
October 18, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29122477/development-and-characterization-of-an-innovative-synbiotic-fermented-beverage-based-on-vegetable-soybean
#20
Carolina Battistini, Beatriz Gullón, Erica Sayuri Ichimura, Ana Maria Pereira Gomes, Eliana Paula Ribeiro, Leo Kunigk, José Ubirajara Vieira Moreira, Cynthia Jurkiewicz
Soymilk was produced from vegetable soybean and fermented by probiotics (Lactobacillus acidophilus La-5, Bifidobacterium animalis Bb-12) in co-culture with Streptococcus thermophilus. The composition of the fermented beverage and oligosaccharides content were determined. The effect of fructooligosaccharides and inulin on the fermentation time and viability of probiotic microorganisms throughout 28 days of storage at 5°C were evaluated. The soymilk from vegetable soybeans was fermented in just 3.2h, when pH reached 4...
October 18, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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