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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/28410799/genomic-identification-and-characterization-of-the-elite-strains-bradyrhizobium-yuanmingense-br-3267-and-bradyrhizobium-pachyrhizi-br-3262-recommended-for-cowpea-inoculation-in-brazil
#1
Jakson Leite, Samuel Ribeiro Passos, Jean Luiz Simões-Araújo, Norma Gouvêa Rumjanek, Gustavo Ribeiro Xavier, Jerri Édson Zilli
The leguminous inoculation with nodule-inducing bacteria that perform biological nitrogen fixation is a good example of an "eco-friendly agricultural practice". Bradyrhizobium strains BR 3267 and BR 3262 are recommended for cowpea (Vigna unguiculata) inoculation in Brazil and showed remarkable responses; nevertheless neither strain was characterized at species level, which is our goal in the present work using a polyphasic approach. The strains presented the typical phenotype of Bradyrhizobium with a slow growth and a white colony on yeast extract-mannitol medium...
March 31, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28365094/high-temperature-ethanol-production-using-thermotolerant-yeast-newly-isolated-from-greater-mekong-subregion
#2
Atiya Techaparin, Pornthap Thanonkeo, Preekamol Klanrit
The application of high-potential thermotolerant yeasts is a key factor for successful ethanol production at high temperatures. Two hundred and thirty-four yeast isolates from Greater Mekong Subregion (GMS) countries, i.e., Thailand, The Lao People's Democratic Republic (Lao PDR) and Vietnam were obtained. Five thermotolerant yeasts, designated Saccharomyces cerevisiae KKU-VN8, KKU-VN20, and KKU-VN27, Pichia kudriavzevii KKU-TH33 and P. kudriavzevii KKU-TH43, demonstrated high temperature and ethanol tolerance levels up to 45°C and 13% (v/v), respectively...
March 18, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28389190/detection-of-vancomycin-resistant-enterococci-vre-in-stool-specimens-submitted-for-clostridium-difficile-toxin-testing
#3
Sevim Özsoy, Arzu İlki
The aim of this study was to determine the association between Clostridium difficile (C. difficile) and vancomycin-resistant Enterococcus (VRE) and efficacy of screening stools submitted for C. difficile toxin assay for prevalence of VRE. Between April 2012 and February 2014, 158 stool samples submitted for C. difficile toxin to the Marmara University Microbiology Laboratory, were included in the study. Stool samples were analyzed by enzyme immuno assay test; VIDAS (bioMerieux, France) for Toxin A&B. Samples were inoculated on chromID VRE (bioMerieux, France) and incubated 24h at 37°C...
March 17, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28359856/evaluation-of-short-chain-length-polyhydroxyalkanoate-accumulation-in-bacillus-aryabhattai
#4
Aneesh Balakrishna Pillai, Arjun Jaya Kumar, Kavitha Thulasi, Harikrishnan Kumarapillai
This study was focused on the polyhydroxybutyrate (PHB) accumulation property of Bacillus aryabhattai isolated from environment. Twenty-four polyhydroxyalkanoate (PHA) producers were screened out from sixty-two environmental bacterial isolates based on Sudan Black B colony staining. Based on their PHA accumulation property, six promising isolates were further screened out. The most productive isolate PHB10 was identified as B. aryabhattai PHB10. The polymer production maxima were 3.264g/L, 2.181g/L, 1.47g/L, 1...
March 11, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28347590/draft-genome-analysis-of-dietzia-sp-111n12-1-isolated-from-the-south-china-sea-with-bioremediation-activity
#5
Shanjun Yang, Mingjia Yu, Jianming Chen
Dietzia sp. 111N12-1, isolated from the seawater of South China Sea, shows strong petroleum hydrocarbons degradation activity. Here, we report the draft sequence of approximately 3.7-Mbp genome of this strain. To the best of our knowledge, this is the first genome sequence of Dietzia strain isolated from the sea. The genome sequence may provide fundamental molecular information on elucidating the metabolic pathway of hydrocarbons degradation in this strain.
March 11, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28283415/fermentation-process-for-production-of-apple-based-kefir-vinegar-microbiological-chemical-and-sensory-analysis
#6
Roberta Oliveira Viana, Karina Teixeira Magalhães-Guedes, Roberto Alves Braga, Disney Ribeiro Dias, Rosane Freitas Schwan
The aim of this study was to develop a kefir apple-based vinegar and evaluate this fermentation process using new methodology with Biospeckle Laser. Brazilian kefir grains were inoculated in apple must for vinegar production. In this study, the microbial community present in kefir, and correspondent vinegar, was investigated using Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) technique. Saccharomyces cerevisiae, Lactobacillus paracasei, Lactobacillus plantarum, Acetobacter pasteurianus and Acetobacter syzygii were the microbial species identified...
March 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28292630/a-plate-method-for-rapid-screening-of-ketogulonicigenium-vulgare-mutants-for-enhanced-2-keto-l-gulonic-acid-production
#7
Weichao Yang, Litao Han, Mandlaa Mandlaa, Haihong Zhang, Zhongze Zhang, Hui Xu
A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94...
February 21, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28279600/development-of-a-novel-compound-microbial-agent-for-degradation-of-kitchen-waste
#8
Kaining Zhao, Rui Xu, Ying Zhang, Hao Tang, Chuanbin Zhou, Aixin Cao, Guozhu Zhao, Hui Guo
Large quantities of kitchen waste are produced in modern society and its disposal poses serious environmental and social problems. The aim of this study was to isolate degradative strains from kitchen waste and to develop a novel and effective microbial agent. One hundred and four strains were isolated from kitchen waste and the 84 dominant strains were used to inoculate protein-, starch-, fat- and cellulose-containing media for detecting their degradability. Twelve dominant strains of various species with high degradability (eight bacteria, one actinomycetes and three fungi) were selected to develop a compound microbial agent "YH" and five strains of these species including H7 (Brevibacterium epidermidis), A3 (Paenibacillus polymyxa), E3 (Aspergillus japonicus), F9 (Aspergillus versicolor) and A5 (Penicillium digitatum), were new for kitchen waste degradation...
February 21, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28320598/molecular-characterization-of-ureaplasmas-isolated-from-reproductive-tract-of-goats-and-sheep-from-brazil
#9
Rosângela C Oliveira, Maurício Yamaguti, Lucas M Marques, Melissa Buzinhani, Huber Rizzo, Enoch Brandão de Souza Meira-Junior, Ana Márcia S Guimarães, Lilian Gregory, Jorge Timenetsky
Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility...
February 20, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28279601/enhanced-ethanol-production-at-commercial-scale-from-molasses-using-high-gravity-technology-by-mutant-s-cerevisiae
#10
Muhammad Arshad, Tariq Hussain, Munawar Iqbal, Mazhar Abbas
Very high gravity (VHG) technology was employed on industrial scale to produce ethanol from molasses (fermented) as well as by-products formation estimation. The effect of different Brix° (32, 36 and 40) air-flow rates (0.00, 0.20, 0.40, and 0.60vvm) was studied on ethanol production. The maximum ethanol production was recorded to be 12.2% (v/v) at 40 Brix° with 0.2vvm air-flow rate. At optimum level aeration and 40 Brix° VHG, the residual sugar level was recorded in the range of 12.5-18.5g/L, whereas the viable cell count remained constant up to 50h of fermentation and dry matter production increased with fermentation time...
February 16, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28262388/enzymatic-comparison-and-mortality-of-beauveria-bassiana-against-cabbage-caterpillar-pieris-brassicae-linn
#11
Manish Dhawan, Neelam Joshi
Beauveria bassiana, an entomopathogenic fungus, is the alternative biocontrol agent exploited against major economic crop pests. Pieris brassicae L. is an emerging pest of the Brassicaceae family. Therefore, in the present study, fungal isolates of Beauveria bassiana, viz. MTCC 2028, MTCC 4495, MTCC 6291, and NBAII-11, were evaluated for their virulence against third instar larvae of P. brassicae. Among all these fungal isolates, maximum mortality (86.66%) was recorded in B. bassiana MTCC 4495 at higher concentration of spores (10(9)conidia/ml), and the minimum mortality (30...
February 16, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28256391/molecular-epidemiology-of-streptococcus-agalactiae-isolated-from-mastitis-in-brazilian-dairy-herds
#12
Glei A Carvalho-Castro, Juliana R Silva, Luciano V Paiva, Dircéia A C Custódio, Rafael O Moreira, Glaucia F Mian, Ingrid A Prado, Antônio Chalfun-Junior, Geraldo M Costa
Streptococcus agalactiae is one of the most common pathogens leading to mastitis in dairy herds worldwide; consequently, the pathogen causes major economic losses for affected farmers. In this study, multilocus sequence typing (MLST), genotypic capsular typing by multiplex polymerase chain reaction (PCR), and virulence gene detection were performed to address the molecular epidemiology of 59 bovine (mastitis) S. agalactiae isolates from 36 dairy farms located in the largest milk-producing mesoregions in Brazil (Minas Gerais, São Paulo, Paraná, and Pernambuco)...
February 16, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28245966/immobilization-of-ammonia-oxidizing-bacteria-by-polyvinyl-alcohol-and-sodium-alginate
#13
Yuwei Dong, Yanqiu Zhang, Baojun Tu
Ammonia-oxidizing bacteria were immobilized by polyvinyl alcohol (PVA) and sodium alginate. The immobilization conditions and ammonia oxidation ability of the immobilized bacteria were investigated. The following immobilization conditions were observed to be optimal: PVA, 12%; sodium alginate, 1.1%; calcium chloride, 1.0%; inoculum concentration, 1.3 immobilized balls/mL of immobilized medium; pH, 10; and temperature, 30°C. The immobilized ammonia-oxidizing bacteria exhibited strong ammonia oxidation ability even after being recycled four times...
February 15, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28245965/prevalence-serotyping-and-antimicrobials-resistance-mechanism-of-salmonella-enterica-isolated-from-clinical-and-environmental-samples-in-saudi-arabia
#14
Mohamed A El-Tayeb, Abdelnasser S S Ibrahim, Ali A Al-Salamah, Khalid S Almaary, Yahya B Elbadawi
Salmonella is recognized as a common foodborne pathogen, causing major health problems in Saudi Arabia. Herein, we report epidemiology, antimicrobial susceptibility and the genetic basis of resistance among S. enterica strains isolated in Saudi Arabia. Isolation of Salmonella spp. from clinical and environmental samples resulted in isolation of 33 strains identified as S. enterica based on their biochemical characteristics and 16S-rDNA sequences. S. enterica serovar Enteritidis showed highest prevalence (39...
February 14, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28365095/human-leptospirosis-occurrence-of-serovars-of-leptospira-spp-in-the-state-of-minas-gerais-brazil-from-2008-to-2012
#15
Marluce Aparecida Assunção Oliveira, Élida Aparecida Leal, Max Assunção Correia, José Carlos Serufo Filho, Ricardo Souza Dias, José Carlos Serufo
BACKGROUND: Leptospirosis is an infectious and acute disease caused by Leptospira spp. that have high epidemic potential. This study verified the main Leptospira spp. serovars detected by MAT from serum of patients with suspicion of leptospirosis from 2008 to 2012 in Minas Gerais State. METHODS: The laboratory received sera from 4654 patients. All serum were screened by IgM-ELISA according to the manufacturer's instructions. Each sample reactive or indeterminate were tested against twenty-four serovars of Leptospira by MAT...
February 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28359855/draft-genome-sequence-of-streptomyces-sp-strain-f1-a-potential-source-for-glycoside-hydrolases-isolated-from-brazilian-soil
#16
Ricardo Rodrigues de Melo, Gabriela Felix Persinoti, Douglas Antonio Alvaredo Paixão, Fábio Márcio Squina, Roberto Ruller, Helia Harumi Sato
Here, we show the draft genome sequence of Streptomyces sp. F1, a strain isolated from soil with great potential for secretion of hydrolytic enzymes used to deconstruct cellulosic biomass. The draft genome assembly of Streptomyces sp. strain F1 has 69 contigs with a total genome size of 8,142,296bp and G+C 72.65%. Preliminary genome analysis identified 175 proteins as Carbohydrate-Active Enzymes, being 85 glycoside hydrolases organized in 33 distinct families. This draft genome information provides new insights on the key genes encoding hydrolytic enzymes involved in biomass deconstruction employed by soil bacteria...
February 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28359854/optimizing-culture-conditions-for-production-of-intra-and-extracellular-inulinase-and-invertase-from-aspergillus-niger-atcc-20611-by-response-surface-methodology-rsm
#17
Mojdeh Dinarvand, Malahat Rezaee, Majid Foroughi
The aim of this study was obtain a model that maximizes growth and production of inulinase and invertase by Aspergillus niger ATCC 20611, employing response surface methodology (RSM). The RSM with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R(2)) more than 0.90 for all responses. This model adequately explained the data variation and represented the actual relationships between the parameters and responses...
February 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28291698/detection-and-analysis-of-different-interactions-between-resistance-mechanisms-and-carbapenems-in-clinical-isolates-of-klebsiella-pneumoniae
#18
Tanise Vendruscolo Dalmolin, Bianca Vendruscolo Bianchini, Grazielle Guidolin Rossi, Ana Carolina Ramos, Ana Cristina Gales, Priscila de Arruda Trindade, Marli Matiko Anraku de Campos
Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28237679/pectin-lyase-overproduction-by-penicillium-griseoroseum-mutants-resistant-to-catabolite-repression
#19
Juliana Oliveira Lima, Jorge Fernando Pereira, Elza Fernandes de Araújo, Marisa Vieira de Queiroz
Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants were obtained. Some mutants produced sectors (segments morphologically different) that were also studied. The mutants were analyzed for pectinases production on pectinase-agar plates and five mutants and two sectors showing larger clearing zones than the wild type were selected for quantitative assay...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28237678/cultivation-of-pichia-pastoris-carrying-the-scfv-anti-ldl-antibody-fragment-effect-of-preculture-carbon-source
#20
Cesar Andres Diaz Arias, Daniela de Araujo Viana Marques, Luciana Pellegrini Malpiedi, Andrea Queiroz Maranhão, Dulcineia Abdalla Saes Parra, Attilio Converti, Adalberto Pessoa Junior
Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically modified to produce the single-chain variable fragment anti low density lipoprotein (-) under the control of the alcohol oxidase promoter have been investigated in this study. In particular, it was evaluated if, and eventually how, the carbon source (glucose or glycerol) used in the preculture preceding cryopreservation in 20% glycerol influences both cell and antibody fragment productions either in flasks or in bioreactor...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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