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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/29631893/illumina-sequencing-and-assessment-of-new-cost-efficient-protocol-for-metagenomic-dna-extraction-from-environmental-water-samples
#1
Mariam Hassan, Tamer Essam, Salwa Megahed
In this study, the development and assessment of a modified, efficient, and cost-efficient protocol for mDNA (metagenomic DNA) extraction from contaminated water samples was attempted. The efficiency of the developed protocol was investigated in comparison to a well-established commercial kit (Epicentre, Metagenomic DNA Isolation Kit for Water). The comparison was in terms of degree of shearing, yield, purity, duration, suitability for polymerase chain reaction and next-generation sequencing in addition to the quality of next-generation sequencing data...
April 3, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29661568/cultivated-bacterial-diversity-associated-with-the-carnivorous-plant-utricularia-breviscapa-lentibulariaceae-from-floodplains-in-brazil
#2
Felipe Rezende Lima, Almir José Ferreira, Cristine Gobbo Menezes, Vitor Fernandes Oliveira Miranda, Manuella Nóbrega Dourado, Welington Luiz Araújo
Carnivorous plant species, such as Utricularia spp., capture and digest prey. This digestion can occur through the secretion of plant digestive enzymes and/or by bacterial digestive enzymes. To comprehend the physiological mechanisms of carnivorous plants, it is essential to understand the microbial diversity related to these plants. Therefore, in the present study, we isolated and classified bacteria from different organs of Utricularia breviscapa (stolons and utricles) and from different geographic locations (São Paulo and Mato Grosso)...
March 31, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29636299/outstanding-impact-of-soil-tillage-on-the-abundance-of-soil-hydrolases-revealed-by-a-metagenomic-approach
#3
Renata Carolini Souza, Maurício Egídio Cantão, Marco Antonio Nogueira, Ana Tereza Ribeiro Vasconcelos, Mariangela Hungria
The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database...
March 31, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29631892/the-potential-of-compounds-isolated-from-xylaria-spp-as-antifungal-agents-against-anthracnose
#4
Luciana M Elias, Diana Fortkamp, Sérgio B Sartori, Marília C Ferreira, Luiz H Gomes, João L Azevedo, Quimi V Montoya, André Rodrigues, Antonio G Ferreira, Simone P Lira
Anthracnose is a crop disease usually caused by fungi in the genus Colletotrichum or Gloeosporium. These are considered one of the main pathogens, causing significant economic losses, such as in peppers and guarana. The current forms of control include the use of resistant cultivars, sanitary pruning and fungicides. However, even with the use of some methods of controlling these cultures, the crops are not free of anthracnose. Additionally, excessive application of fungicides increases the resistance of pathogens to agrochemicals and cause harm to human health and the environment...
March 31, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29609849/occurrence-of-serological-reactions-for-serogroup-sejroe-ctg-and-prajtino-in-female-buffalo-in-the-state-of-pernambuco-brazil
#5
Pollyanne Raysa Fernandes de Oliveira, Larice Bruna Ferreira Soares, Jonas de Melo Borges, Noelle de Castro Barrosa, Hélio Langoni, Daniel Friguglietti Brandespim, José Wilton Pinheiro Junior, Rinaldo Aparecido Mota
The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.7-37.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results...
March 20, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29598976/phylogenetic-mlsa-and-phenotypic-analysis-identification-of-three-probable-novel-pseudomonas-species-isolated-on-king-george-island-south-shetland-antarctica
#6
Felipe Vásquez-Ponce, Sebastián Higuera-Llantén, María S Pavlov, Sergio H Marshall, Jorge Olivares-Pacheco
Antarctica harbors a great diversity of microorganisms, including bacteria, archaea, microalgae and yeasts. The Pseudomonas genus is one of the most diverse and successful bacterial groups described to date, but only eight species isolated from Antarctica have been characterized. Here, we present three potentially novel species isolated on King George Island. The most abundant isolates from four different environments, were genotypically and phenotypically characterized. Multilocus sequence analysis and 16S rRNA gene analysis of a sequence concatenate for six genes (16S, aroE, glnS, gyrB, ileS and rpoD), determined one of the isolates to be a new Pseudomonas mandelii strain, while the other three are good candidates for new Pseudomonas species...
March 15, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29588198/identification-of-enteric-viruses-circulating-in-a-dog-population-with-low-vaccine-coverage
#7
Christian D B T Alves, Oscar F O Granados, Renata da F Budaszewski, André F Streck, Matheus N Weber, Samuel P Cibulski, Luciane D Pinto, Nilo Ikuta, Cláudio W Canal
Although the use of vaccines has controlled enteric diseases in dogs in many developed countries, vaccine coverage is still under optimal situation in Brazil. There is a large population of nonimmunized dogs and few studies about the identification of the viruses associated with diarrhea. To address this situation, stool samples from 325 dogs were analyzed by polymerase chain reaction for the detection of common enteric viruses such as Canine adenovirus (CAdV), Canine coronavirus (CCoV), Canine distemper virus (CDV), Canine rotavirus (CRV) and Carnivorous protoparvovirus 1 (canine parvovirus 2; CPV-2)...
March 15, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29588197/screening-of-medium-constituents-for-clavulanic-acid-production-by-streptomyces-clavuligerus
#8
Kaio César da Silva Rodrigues, Arianne Tairyne de Souza, Alberto Colli Badino, Danielle Biscaro Pedrolli, Marcel Otavio Cerri
Clavulanic acid is a β-lactam compound with potent inhibitory activity against β-lactamases. Studies have shown that certain amino acids play essential roles in CA biosynthesis. However, quantitative evaluations of the effects of these amino acids are still needed in order to improve CA production. Here, we report a study of the nutritional requirements of Streptomyces clavuligerus for CA production. Firstly, the influence of the primary nitrogen source and the salts composition was investigated. Subsequently, soybean protein isolate was supplemented with arginine (0...
March 15, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29551641/histophilus-somni-associated-syndromes-in-sheep-from-southern-brazil
#9
Selwyn A Headley, Alfredo H T Pereira, Luciana C Balbo, Giovana W Di Santia, Ana P F R L Bracarense, Luiz F C Cunha Filho, Jackson Schade, Werner Okano, Priscilla F V Pereira, Fábio Morotti, Lucienne G Preto-Giordano, Rogério A Marcasso, Alice F Alfieri, Júlio A N Lisbôa, Amauri A Alfieri
Histophilus somni is a Gram-negative bacterium that is associated with a disease complex (termed histophilosis) that can produce several clinical syndromes predominantly in cattle, but also in sheep. Histophilosis is well described in North America, Canada, and in some European countries. In Brazil, histophilosis has been described in cattle with respiratory, reproductive, and systemic disease, with only one case described in sheep. This report describes the occurrence of Histophilus somni-associated disease in sheep from Southern Brazil...
March 15, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29606509/antimicrobial-susceptibility-and-fluctuations-in-clonal-complexes-of-serogroup-6-streptococcus-pneumoniae-isolates-collected-from-children-in-beijing-china-between-1997-and-2016
#10
Wei Shi, Ying Liu, Qinghong Meng, Lin Yuan, Wei Gao, Kaihu Yao
This study examined the antimicrobial susceptibility patterns and clonal complex (CC) characteristics of serogroup 6 Streptococcus pneumoniae isolates collected from children in Beijing, China, between 1997 and 2016. Serotypes were determined using the Quellung reaction, and the antimicrobial susceptibility profiles of the isolates were determined using the disc-diffusion method or by E-test. Sequence types (STs) were assigned based on multilocus sequence typing. A total of 250 isolates were examined, with 55...
March 14, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29588199/is-a-dose-of-17d-vaccine-in-the-current-context-of-yellow-fever-enough
#11
LETTER
Cassia Fernanda Estofolete, Mauricio Lacerda Nogueira
No abstract text is available yet for this article.
March 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29588196/ethanol-production-from-sweet-sorghum-by-saccharomyces-cerevisiae-dbkkuy-53-immobilized-on-alginate-loofah-matrices
#12
Sunan Nuanpeng, Sudarat Thanonkeo, Preekamol Klanrit, Pornthap Thanonkeo
Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed. As found in this study, an ALM with dimensions of 20×20×5mm3 is effective for cell immobilization due to its compact structure and long-term stability. The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system. By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined...
March 13, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29598975/use-of-modified-richards-model-to-predict-isothermal-and-non-isothermal-microbial-growth
#13
Jhony Tiago Teleken, Alessandro Cazonatto Galvão, Weber da Silva Robazza
Mathematical models are often used to predict microbial growth in food products. An important class of these models involves the adaptation of classical sigmoid functions, such as the Gompertz and logistic functions. This study aimed to validate the use of the modified Richards model in various situations, which have not previously been tested. The model was obtained through solving a system of two differential equations and could be applied to both isothermal and non-isothermal environments. To test and validate this model, we used published datasets containing data for the growth of Pseudomonas spp...
March 7, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29606508/isolation-of-salmonella-spp-in-cattle-egrets-bubulcus-ibis-from-fernando-de-noronha-archipelago-brazil
#14
Marcio A Silva, Érika F S T Fernandes, Sandra C Santana, Maria Fernanda V Marvulo, Mércia R Barros, Sineide M O Vilela, Eliane M F Reis, Rinaldo A Mota, Jean C R Silva
The growth of the population of cattle egrets (Bubulcus ibis) in the archipelago of Fernando de Noronha constitutes a threat to public health and biological diversity because of their competition with and predation on native species and the possibility of transmission of pathogens to human beings, livestock and native wildlife. The aim here was to search for, isolate and identify serovars of Salmonella in clinically healthy local cattle egrets. Cloacal swabs were obtained from 456 clinically healthy cattle egrets of both sexes and a variety of ages...
March 3, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29551640/composition-of-bacterial-community-in-enrichment-cultures-of-shale-by-products-from-irati-formation-brazil
#15
Kelly Campos Guerra Pinheiro de Goes, Gisele Milani Lovato, Diva S Andrade
We examined microbial communities from enriched fine and retorted shale particles using sequencing of V4 variable region of 16S rRNA. High number of microbial genera was found in both enriched shale by-products that were dominate by Actinobacteria, Firmicutes and Proteobacteria, showing differences due to microbial colonization after the pyrolysis process.
March 3, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29548716/performance-of-resist-3-o-k-n-k-set-immunochromatographic-assay-for-the-detection-of-oxa-48-like-kpc-and-ndm-carbapenemases-in-klebsiella-pneumoniae-in-turkey
#16
Pınar Sağıroğlu, Ufuk Hasdemir, Gülşen Altınkanat Gelmez, Burak Aksu, Onur Karatuna, Güner Söyletir
In this study, the performance of the "RESIST-3 O.K.N. K-SeT" (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n=22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for blaKPC , blaIMP , blaVIM , blaNDM , and blaOXA-48 ...
March 1, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29548715/phenotype-driven-strategies-for-screening-candida-parapsilosis-complex-for-molecular-identification
#17
Rossana A Cordeiro, Jamille A Sales, Yago B de Ponte, Patrícia B L Mendes, Rosana Serpa, Antônio J Evangelista, Lucas P de Alencar, Waldemiro de A Pereira-Neto, Raimunda S N Brilhante, José J C Sidrim, Débora S C M Castelo-Branco, Marcos F G Rocha
In this study, phenotypic methods presented >80% agreement with the molecular identification of 59 Candida parapsilosis complex. Growth at 15% NaCl or pH 7.0 significantly reduced cfu-counts of Candida orthopsilosis, suggesting these conditions may support the development of phenotypic methods for the differentiation of the cryptic species of C. parapsilosis complex.
March 1, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29544983/prostaglandin-a-1-inhibits-the-replication-of-bovine-viral-diarrhea-virus
#18
Lúcio Ayres Caldas, Tânia Rosária Pereira Freitas, Renata Campos Azevedo, Wanderley de Souza
Bovine viral diarrhea virus can cause acute disease in livestock, leading to economic losses. We show that Prostaglandin A1 inhibits bovine viral diarrhea virus replication in Madin-Darby bovine kidney cells (94% inhibition using 5μg/mL). Light and electron microscopy of infected cells shows that Prostaglandin A1 also prevents virus-induced vacuolization, but at higher concentrations (10μg/mL).
March 1, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29548718/molecular-characterization-of-feline-calicivirus-variants-from-multicat-household-and-public-animal-shelter-in-rio-de-janeiro-brazil
#19
Joylson de Jesus Pereira, Natasha Baumworcel, Júlia Monassa Fioretti, Cinthya Fonseca Domingues, Laís Fernandes de Moraes, Robson Dos Santos Souza Marinho, Maria Clara Rodrigues Vieira, Ana Maria Viana Pinto, Tatiana Xavier de Castro
The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic...
February 28, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29548717/selection-of-starter-cultures-for-the-production-of-sour-cassava-starch-in-a-pilot-scale-fermentation-process
#20
Fernanda Corrêa Leal Penido, Fernanda Barbosa Piló, Sávio Henrique de Cicco Sandes, Álvaro Cantini Nunes, Gecernir Colen, Evelyn de Souza Oliveira, Carlos Augusto Rosa, Inayara Cristina Alves Lacerda
Sour cassava starch (Polvilho azedo) is obtained from a spontaneous fermentation conducted by microorganisms from raw materials and fermentation tanks. This product is traditionally used in the baking industry for the manufacture of biscuits and Brazilian cheese breads. However, the end of fermentation is evaluated empirically, and the process occurs without standardization, which results in products of inconsistent quality. Predominant microbiota from a cassava flour manufacturer was isolated in order to select starter cultures for the production of sour cassava starch in a pilot-scale fermentation process...
February 28, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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