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Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]

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https://www.readbyqxmd.com/read/28720432/adhesion-and-invasion-of-clostridium-perfringens-type-a-into-epithelial-cells
#1
Luis A Llanco, Viviane Nakano, Claudia T P de Moraes, Roxane M F Piazza, Mario J Avila-Campos
Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated...
July 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28689814/isolation-and-characterization-of-a-novel-endo-%C3%AE-1-4-glucanase-from-a-metagenomic-library-of-the-black-goat-rumen
#2
Yun-Hee Song, Kyung-Tai Lee, Jin-Young Baek, Min-Ju Kim, Mi-Ra Kwon, Young-Joo Kim, Mi-Rim Park, Haesu Ko, Jin-Sung Lee, Keun-Sung Kim
The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized...
June 26, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28716388/identification-of-co-infection-by-rotavirus-and-parvovirus-in-dogs-with-gastroenteritis-in-mexico
#3
Ariadna Flores Ortega, José Simón Martínez-Castañeda, Linda G Bautista-Gómez, Raúl Fajardo Muñoz, Israel Quijano Hernández
This is the first report on circulating canine rotavirus in Mexico. Fifty samples from dogs with gastroenteritis were analyzed used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were infected with rotavirus exclusively, while 14% were co-infected with both rotavirus and parvovirus; clinical signs in co-infected dogs were more severe.
June 24, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28689813/combination-of-commercially-available-molecular-assays-and-culture-based-methods-in-diagnosis-of-tuberculosis-and-drug-resistant-tuberculosis
#4
Lamprini Gkaravela, Matthaios Papadimitriou-Olivgeris, Antigoni Foka, Fevronia Kolonitsiou, Anastasia Spiliopoulou, Nikolaos Charokopos, Apostolos Voulgaridis, Maria Tsiamita, Markos Marangos, Evangelos D Anastassiou, Iris Spiliopoulou
Early diagnosis of tuberculosis is of major clinical importance. Among 4733 clinical specimens collected from 3363 patients and subjected to Ziehl-Neelsen microscopy, 4109 were inoculated onto Löwenstein-Jensen slants and 3139 in Bactec/9000MB. Polymerase Chain Reaction (PCR) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 Mycobacterium tuberculosis complex PCR-positive for isoniazid and rifampicin resistance detection specimens (GenoType MTBDRplus). Recovered M. tuberculosis isolates (64) as well as, 21 more sent from Regional Hospitals were tested for antimycobacterial resistance with a phenotypic (manual MGIT-SIRE) and a genotypic assay (GenoType MTBDRplus)...
June 24, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28651890/isolation-and-molecular-characterization-of-thraustochytrium-strain-isolated-from-antarctic-peninsula-and-its-biotechnological-potential-in-the-production-of-fatty-acids
#5
Esteban Caamaño, Lyliam Loperena, Ivonne Hinzpeter, Paulina Pradel, Felipe Gordillo, Gino Corsini, Mario Tello, Paris Lavín, Alex R González
Thraustochytrids are unicellular protists belonging to the Labyrinthulomycetes class, which are characterized by the presence of a high lipid content that could replace conventional fatty acids. They show a wide geographic distribution, however their diversity in the Antarctic Region is rather scarce. The analysis based on the complete sequence of 18S rRNA gene showed that strain 34-2 belongs to the species Thraustochytrium kinnei, with 99% identity. The total lipid profile shows a wide range of saturated fatty acids with abundance of palmitic acid (16:0), showing a range of 16...
June 10, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28645649/evaluation-of-a-selective-chromogenic-medium-for-detecting-vancomycin-resistant-enterococci
#6
Renata Oliveira Soares, Adriana Medianeira Rossato, Gustavo Enck Sambrano, Neidimar Cezar Corrêa Tolfo, Juliana Caierão, Thiago Galvão da Silva Paim, Pedro Alves d'Azevedo
Rapid identification of vancomycin-resistant enterococci (VRE) can assist in choosing the appropriate treatment and preventing VRE spread. The performance of chromID™ VRE agar was evaluated using 184 clinical isolates of Enterococcus spp. and reference strains. The test had a sensitivity of 95.52% but a low specificity of 30%.
June 8, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28648636/contribution-of-flagella-and-motility-to-gut-colonisation-and-pathogenicity-of-salmonella-enteritidis-in-the-chicken
#7
Fernanda de Oliveira Barbosa, Oliveiro Caetano de Freitas Neto, Diego Felipe Alves Batista, Adriana Maria de Almeida, Marcela da Silva Rubio, Lucas Bocchini Rodrigues Alves, Rosemeire de Oliveira Vasconcelos, Paul Andrew Barrow, Angelo Berchieri Junior
Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes...
June 7, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28645648/promotion-of-iron-nutrition-and-growth-on-peanut-by-paenibacillus-illinoisensis-and-bacillus-sp-strains-in-calcareous-soil
#8
Di Liu, Qianqian Yang, Ke Ge, Xiuna Hu, Guozhen Qi, Binghai Du, Kai Liu, Yanqin Ding
This study aimed to explore the effects of two siderophore-producing bacterial strains on iron absorption and plant growth of peanut in calcareous soil. Two siderophore-producing bacterial strains, namely, YZ29 and DZ13, isolated from the rhizosphere soil of peanut, were identified as Paenibacillus illinoisensis and Bacillus sp., respectively. In potted experiments, YZ29 and DZ13 enhanced root activity, chlorophyll and active iron content in leaves, total nitrogen, phosphorus and potassium accumulation of plants and increased the quality of peanut kernels and plant biomass over control...
June 4, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28651889/a-novel-expression-vector-for-the-secretion-of-abaecin-in-bacillus-subtilis
#9
Li Li, Lan Mu, Xiaojuan Wang, Jingfeng Yu, Ruiping Hu, Zhen Li
This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28647306/complete-genome-sequence-of-a-phthalic-acid-esters-degrading-mycobacterium-sp-yc-rl4
#10
Lei Ren, Shuanghu Fan, Junhuan Wang, Nahurira Ruth, Cheng Qiao, Yang Jia, Yanchun Yan
Mycobacterium sp. YC-RL4 is capable of utilizing a broad range of phthalic acid esters (PAEs) as sole source of carbon and energy for growth. The preliminary studies demonstrated its high degrading efficiency and good performance during the bioprocess with environmental samples. Here, we present the complete genome of Mycobacterium sp. YC-RL4, which consists of one circular chromosome (5,801,417bp) and one plasmid (252,568bp). The genomic analysis and gene annotation were performed and many potential genes responsible for the biodegradation of PAEs were identified from the genome...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28645650/isolation-identification-and-biocontrol-of-antagonistic-bacterium-against-botrytis-cinerea-after-tomato-harvest
#11
Jun-Feng Shi, Chang-Qing Sun
Tomato is one of the most important vegetables in the world. Decay after harvest is a major issue in the development of tomato industry. Currently, the most effective method for controlling decay after harvest is storage of tomato at low temperature combined with usage of chemical bactericide; however, long-term usage of chemical bactericide not only causes pathogen resistance but also is harmful for human health and environment. Biocontrol method for the management of disease after tomato harvest has great practical significance...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28641956/antibacterial-efficacy-of-nisin-bacteriophage-p100-and-sodium-lactate-against-listeria-monocytogenes-in-ready-to-eat-sliced-pork-ham
#12
Ana Cláudia L Figueiredo, Rogeria C C Almeida
The effectiveness of bacteriophage P100, nisin and sodium lactate, individually and in combination, in inhibiting Listeria monocytogenes in ready-to-eat pork ham slices was assessed. The antimicrobials were applied to the surfaces of ready-to-eat pork ham slices, which were inoculated with a mixture of L. monocytogenes. Among the individual antimicrobial treatments, bacteriophage P100 was the most effective, decreasing L. monocytogenes to undetectable levels at zero and 72h post-infection. Sodium lactate was the least effective treatment...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28633981/development-of-a-propidium-monoazide-polymerase-chain-reaction-assay-for-detection-of-viable-lactobacillus-brevis-in-beer
#13
Yanlin Ma, Yang Deng, Zhenbo Xu, Junyan Liu, Jianjun Dong, Hua Yin, Junhong Yu, Zongming Chang, Dongfeng Wang
The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this paper, the combination of propidium monoazide (PMA) pretreatment and conventional PCR had been described. The established PMA-PCR identified beer spoilage Lactobacillus brevis based not on their identity, but on the presence of horA gene which we show to be highly correlated with the ability of beer spoilage LAB to grow in beer...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629972/characterization-of-dioxygenases-and-biosurfactants-produced-by-crude-oil-degrading-soil-bacteria
#14
Santhakumar Muthukamalam, Sivalingam Sivagangavathi, Dharmapal Dhrishya, Sadras Sudha Rani
Role of microbes in bioremediation of oil spills has become inevitable owing to their eco friendly nature. This study focused on the isolation and characterization of bacterial strains with superior oil degrading potential from crude-oil contaminated soil. Three such bacterial strains were selected and subsequently identified by 16S rRNA gene sequence analysis as Corynebacterium aurimucosum, Acinetobacter baumannii and Microbacterium hydrocarbonoxydans respectively. The specific activity of catechol 1,2 dioxygenase (C12O) and catechol 2,3 dioxygenase (C23O) was determined in these three strains wherein the activity of C12O was more than that of C23O...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629971/survey-of-pathogens-in-threatened-wild-red-tailed-amazon-parrot-amazona-brasiliensis-nestlings-in-rasa-island-brazil
#15
Frederico Fontanelli Vaz, Patrícia Pereira Serafini, Rosangela Locatelli-Dittrich, Rafael Meurer, Edison Luiz Durigon, Jansen de Araújo, Luciano Matsumiya Thomazelli, Tatiana Ometto, Elenise Angelotti Bastos Sipinski, Rafael Meirelles Sezerban, Maria Cecília Abbud, Tânia Freitas Raso
The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629969/prevalence-and-serotype-distribution-of-listeria-monocytogenes-isolated-from-foods-in-montevideo-uruguay
#16
Valeria Braga, Sylvia Vázquez, Victoria Vico, Valeria Pastorino, María Inés Mota, Marcela Legnani, Felipe Schelotto, Gustavo Lancibidad, Gustavo Varela
The aim of this work was to study the prevalence of Listeria monocytogenes in foods obtained in retail shops and food industries located in Montevideo-Uruguay, and to identify the serogroups of the obtained isolates. Three-thousand one-hundred and seventy-five food samples (frozen, deli meats, ready-to-eat and cheese) were analyzed. The obtained isolates were serogrouped by multiplex PCR and serotyped by conventional procedure. Genetic comparisons were performed using pulsed-field gel electrophoresis on a sub-set of isolates belonging to the same serotype successively recovered from the same establishment...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629968/inhibitor-tolerance-of-a-recombinant-flocculating-industrial-saccharomyces-cerevisiae-strain-during-glucose-and-xylose-co-fermentation
#17
Yun-Cheng Li, Zi-Xi Gou, Ying Zhang, Zi-Yuan Xia, Yue-Qin Tang, Kenji Kida
Lignocellulose-derived inhibitors have negative effects on the ethanol fermentation capacity of Saccharomyces cerevisiae. In this study, the effects of eight typical inhibitors, including weak acids, furans, and phenols, on glucose and xylose co-fermentation of the recombinant xylose-fermenting flocculating industrial S. cerevisiae strain NAPX37 were evaluated by batch fermentation. Inhibition on glucose fermentation, not that on xylose fermentation, correlated with delayed cell growth. The weak acids and the phenols showed additive effects...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28629967/isolation-of-fungi-from-dung-of-wild-herbivores-for-application-in-bioethanol-production
#18
Rhulani Makhuvele, Ignatious Ncube, Elbert Lukas Jansen van Rensburg, Daniël Coenrad La Grange
Producing biofuels such as ethanol from non-food plant material has the potential to meet transportation fuel requirements in many African countries without impacting directly on food security. The current shortcomings in biomass processing are inefficient fermentation of plant sugars, such as xylose, especially at high temperatures, lack of fermenting microbes that are able to resist inhibitors associated with pre-treated plant material and lack of effective lignocellulolytic enzymes for complete hydrolysis of plant polysaccharides...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28625768/screening-and-characterization-of-lactic-acid-bacterial-strains-that-produce-fermented-milk-and-reduce-cholesterol-levels
#19
Xuefang Guan, Qingxian Xu, Yi Zheng, Lei Qian, Bin Lin
OBJECTIVE: To screen for and characterize lactic acid bacteria strains with the ability to produce fermented milk and reduce cholesterol levels. METHODS: The strains were isolated from traditional fermented milk in China. In vitro and in vivo evaluation of cholesterol-reduction were used to identify and verify strains of interest. Characteristics were analyzed using spectrophotometry and plate counting assays. RESULTS: The isolate HLX37 consistently produced fermented milk with strong cholesterol-reducing properties was identified as Lactobacillus plantarum (accession number: KR105940) and was thus selected for further study...
June 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28619663/captive-wild-birds-as-reservoirs-of-enteropathogenic-e-coli-epec-and-shiga-toxin-producing-e-coli-stec
#20
Lilian Aparecida Sanches, Marcelo da Silva Gomes, Rodrigo Hidalgo Friciello Teixeira, Marcos Paulo Vieira Cunha, Maria Gabriela Xavier de Oliveira, Mônica Aparecida Midolli Vieira, Tânia Aparecida Tardelli Gomes, Terezinha Knobl
Psittacine birds have been identified as reservoirs of diarrheagenic Escherichia coli, a subset of pathogens associated with mortality of children in tropical countries. The role of other orders of birds as source of infection is unclear. The aim of this study was to perform the molecular diagnosis of infection with diarrheagenic E. coli in 10 different orders of captive wild birds in the state of São Paulo, Brazil. Fecal samples were analyzed from 516 birds belonging to 10 orders: Accipitriformes, Anseriformes, Columbiformes, Falconiformes, Galliformes, Passeriformes, Pelecaniformes, Piciformes, Psittaciformes and Strigiformes...
June 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
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