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Artificial Cells, Nanomedicine, and Biotechnology

Xiaofeng Yin, Xiaomeng Zhang, Yuhui Kou, Yanhua Wang, Lijia Zhang, Baoguo Jiang, Dianying Zhang
Using portion of a nearby nerve trunk to reconstruct a severe nerve lesion by artificial biodegradable chitin conduit is the core practicable method based on peripheral nerve amplification regeneration. However, the quantitative influences on skeletal muscle function corresponding to the injury of the donated nerve fibres were not previously reported. Here, we aimed to explore the compensative capacity in tibialis anterior muscles of rats with the models of acute tibialis anterior nerve branch injuries. The tibialis anterior branch of deep peroneal nerve was transected in various levels each time...
July 16, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Krushna C Hembram, Rahul Kumar, Laxman Kandha, Pankaj K Parhi, Chanakya N Kundu, Birendra K Bindhani
Green synthesis approach for nanoparticle is environmental friendly, non-hazardous and the nanoparticles have shown enhanced biocompatibility for application in healthcare. Previous reviews have mentioned about green synthesis methods for nanoparticles and their biological activities. This review not only covers the general information about green synthesis of silver nanoparticles and characterization, but also focused on recent uses of various medicinal and nonmedical plants based AgNPs synthesis and their broad-spectrum antimicrobial and anticancer activities...
July 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Chunyan Yue, Manyi Yang, Qinggang Tian, Fongming Mo, Jian Peng, Yan Ma, Yanning Huang, Dongcui Wang, Yuehua Wang, Zhiyuan Hu
Insulin-like growth factor-binding protein 7 (IGFBP7) is a secreted protein and its expression was restrained in varied solid tumours, but there was no report about biological role of IGFBP7 in cholangiocarcinoma (CCA). Here, we found that high expression of IGFBP7 correlated to a better overall survival in CCA patients. To investigate the hypothetic antineoplastic activity of IGFBP7 in CCA, we induced overexpression of IGFBP7 in QBC939 and RBE cells, as well as knockdown in HCCC9810 cells. And the biological functions triggered by level changes of IGFBP7 were assessed, including proliferation, cell cycle distribution, apoptosis and invasion evaluation...
July 10, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Md Habban Akhter, Nv Sateesh Madhav, Javed Ahmad
The epidermal growth factor receptor (EGFR) is a cell surface receptor belonging to erythroblastic leukemia viral oncogene homologue (ErbB) family of tyrosine kinase. It plays critical role in the regulation of cell proliferation, survival and differentiation. The EGFR receptor is crucial in a variety of tumor development due to unlikely triggered by receptor overexpression, chromosomal mutation and or ligand-dependent receptor dimerization. The EGFR inhibition established a major therapeutic target in cancer therapy...
July 10, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Parisa Nasrollahi, Khosro Khajeh, Elnaz Tamjid, Mohammad Taleb, Masoud Soleimani, Guangjun Nie
Delivery of the drugs to the target tissue and reducing their side effects on surrounding tissues is still a significant challenge for pharmaceutical scientists. The aim of this study was to investigate the suitability of PLGA-PEG-PLGA triblock copolymer as a matrix material for a sustained-release system of sodium deoxycholate (NaDC). The copolymer was synthesized by ring-opening polymerization reaction, using microwave irradiation and characterized by different techniques. It was shown that the introduction of NaDC to the PLGA-PEG-PLGA copolymer did not influence its inherent sol-gel transition behaviour, but increased the sol-gel transition...
July 10, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Rui Yu, Sai Cao, Yanhong Liu, Xinxi Si, Ting Fang, Xu Sun, Hongmei Dai, Junjie Xu, Hongqing Fang, Wei Chen
Thymosin β4 (Tβ4) is a multifunctional N-acetylated peptide with distinct activities important at various stages. Due to its potential multiple therapeutic uses in many fields, there is an increasing need of Tβ4 at lower costs than with the use of chemical synthesis. In this research, we developed a method to produce rhTβ4 with N-acetylation in E. coli. Firstly, the E. coli strain whose chromosome being integrated by the specific N-terminal acetyltransferase ssArd1 was constructed. Secondly, the rhTβ4-Intein was constructed, in which rhTβ4 was fused to the N-terminus of the smallest mini-intein Spl DnaX...
July 10, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Yan Zhang, Shuang Ren, Fengfeng Yuan, Kaishang Zhang, Yingying Fan, Shaozhong Zheng, Zhen Gao, Jia Zhao, Teng Mu, Song Zhao, AnQuan Shang, Xiangnan Li, Ying Jie
MicroRNA (miRNA) plays an important role in tumourigenesis and cancer development by regulating oncogenes or tumour suppressor that are implicated in cell cycle, cell mobility and even cell senescence. Due to the resistance to enzymes that could degrade nucleotides, miRNAs have been considered proper for diagnosis and prognosis evaluation of cancer. The present study was designed to investigate miRNA associated with ESCC and identified effective miRNAs, which could serve as biomarker or targets. We first performed miRNA profiling to identify a subset of dysregulated miRNAs in ESCC...
July 2, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Gang Chen, T M S Chang
The oxidation reactions have become the main obstacle of development of bovine hemoglobin-derivates products. Herein, the effects of vitamin C (Vc), a easily available natural antioxidant reagent, on the redox reaction of bovine hemoglobin were systematically investigated through methemoglobin (MetHb) formation and spectrophotometric analysis and oxygen affinity monitoring of hemoglobin. The results showed that Vc presented antioxidant effects in the initial stage of reaction and then could accelerated the MetHb content increasing by production of hydrogen peroxide, which can be indirectly characterized by the formation of choleglobin in the following side reactions...
July 2, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Rajeev Sharma, Surabhi Dubey, Nishi Mody, Gajanand Sharma, Varun Kushwah, Sanyog Jain, Om Prakash Katare, Suresh P Vyas
The aim of present approach was to design and develop mannose functionalized reverse polymeric nanocomposite(s) system based on release promoter (MRPRPNs). Thus, the composition of the present formulation was optimized by employing the systematic design of experiments (DoE) for screening and optimization using L8-Array Taguchi and 3-level-3-factor Box-Behnken Design (BBD). Further, the developed formulation was observed for its preferential internalization by professional antigen presenting cells (macrophages/dendritic cells) and prompt release of loaded antigen in a pH-dependent manner...
July 2, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Ricardo Duque, Yiming Shan, Monica Joya, Nandini Ravichandran, Berok Asi, Maryam Mobed-Miremadi, Scott Mulrooney, Melanie McNeil, Satya Prakash
Second generation E. coli DH5α (pKAU17) was successfully encapsulated by means of atomization (MA), inkjet printing (MI) and double-encapsulation (DDMI) for the purpose of urea degradation in a simulated uremic medium at 37 °C. Experimentally determined values of the effectiveness factor are 0.83, 0.28 and 0.34 for the MI, MA and DDMI capsules, respectively, suggesting that the catalytic activity of the E. coli DH5α (pKAU17) immobilized in MI capsule (d = 52 μm ± 2.7 μm) is significantly less diffusion-limited than in the case of the MA (d = 1558 μm ± 125 μm) and DDMI (d = 1370 μm ± 60 μm) bio-encapsulation schemes at the 98...
July 2, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Junyi Shen, Zhiming Zhao, Wei Shang, Chunli Liu, Beibei Zhang, Zihan Xu, Hui Cai
Cerebral infarction is with poorer prognosis and high rates of mortality. After cerebral infarction, the promoting angiogenesis can accelerate the recovery of neurological function. Long non-coding RNA (LncRNA) maternally expressed gene 3 (Meg3) was overexpressed in cerebral infarction area and the knockdown of Meg3 promotes neovascularization and improves nerve function. In this study, we fabricated a nano-polymer wrapped Meg3 short hairpin RNA (ShRNA) plasmid to knockdown Meg3 and conjugated with OX26 antibody (MPO) to realize the brain targeting for the treatment of cerebral infarction...
June 29, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Eun-Young Ahn, You Jeong Lee, Seo Young Choi, A-Rang Im, Yeong Shik Kim, Youmie Park
Skate (Dipturus chilensis) cartilage extract was utilized as a green reducing agent for the synthesis of spherical gold nanoparticles with an average size of 16.7 ± 0.2 nm. The gold nanoparticle solution showed a surface plasmon resonance at 543 nm with a wine-red colour. A strong X-ray diffraction pattern and clear lattice structure in high-resolution transmission electron microscopy indicated a face-centred cubic structure of the gold nanoparticles. The gold nanoparticles retained excellent colloidal stability...
June 29, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Eleonore Fröhlich
Humans are exposed to a wide variety of nanoparticles (NPs) present in the environment, in consumer, health and medical products, and in food. Conventional cytotoxicity testing compared to animal testing is less expensive, faster and avoids ethical problems at the expense of a lower predictive value. New cellular models and exposure conditions have been developed to overcome the limitations of conventional cell culture and obtain more predictive data. The use of three-dimensional culture, co-culture and inclusion of mechanical stimulation can provide physiologically more relevant culture conditions...
June 29, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Xiaojie Chu, Yang Li, Weiwei Huang, Xuejun Feng, Pengyan Sun, Yufeng Yao, Xu Yang, Wenjia Sun, Hongmei Bai, Cunbao Liu, Yanbing Ma
Therapeutic vaccine appears to be a potential approach for the treatment of human papillomavirus (HPV)-associated tumours, but its efficacy can be dampened by immunosuppressive factors such as transforming growth factor (TGF)-β1. We sought to investigate whether active immunity against TGF-β1 enhances the anti-tumour immunity elicited by an HPV16 E7-specific vaccine that we developed previously. In this study, virus-like particles of hepatitis B virus core antigen were used as vaccine carriers to deliver either TGF-β1 B cell epitopes or E7 cytotoxic T-lymphocyte epitope...
June 22, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Saeed Jahandideh, Shohreh Khatami, Ali Eslami Far, Mehdi Kadivar
Systemic inflammatory response syndrome is a complex pathophysiologic and immunologic response to an insult. Sepsis is a life-threatening condition happening when the body's response to infection causes injury to its own tissues and organs. Stem cell therapy is a new approach to modulate immune responses. Mesenchymal stem cells (MSCs) establish a regenerative niche by secreting secretome and modulating immune responses. MSC secretome can be leveraged for therapeutic applications if production of secretary molecules were optimized...
June 22, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Zhihua Chen, Mengzhen Zhang, Yuhuan Qiao, Junjuan Yang, Qinan Yin
BACKGROUND: The human cervical carcinoma oncogenic mechanisms still remain elusive. Thus, we proposed to understand the biological role of a newly discovered therapeutic miRNA. METHODS: MiR-1297 related to human cervical carcinoma was selected for this study. TaqMan qRT- PCR assay was used to profile miRNA, phosphatase and tensin homolog (PTEN) expression in randomly chosen tumour with non-tumour tissues, and the apoptosis factors expression. Cell proliferation was monitored by CCK-8 assay and colony formation assay...
June 19, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Muhammed Anwary, Pradeep Kumar, Lisa C du Toit, Yahya E Choonara, Viness Pillay
Posterior segment diseases are commonly implicated in the occurrence of curable as well as incurable diseases which could lead to blindness. An example of one such disease is age-related macular degeneration, which would require chronic treatment over an extended period and as such the development of an intravitreal drug delivery system that would optimize patient outcomes is essential. The major contributor of the difficulty in treating diseases in the posterior segment is the structure of the eye, particularly the blood-ocular barriers...
June 19, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Kyle Spivack, Matthew Tucker, Devon Zimmerman, Matthew Nicholas, Osheiza Abdulmalik, Noelle Comolli, Jacob Elmer
Since donated red blood cells must be constantly refrigerated, they are often unavailable in remote areas and battlefields. The goal of this study was to synthesize a highly stable blood substitute that does not require refrigeration. Specifically, the extracellular haemoglobin (a.k.a. erythrocruorin, Ec) of the earthworm Lumbricus terrestris erythrocruororin (LtEc) was cross-linked with poly(acrylic acid) (PAA) and ethylene diamine (EDA). PAGE analysis of the LtEc nanoparticles reveals cross-linking between subunits, while dynamic light scattering and scanning electron microscopy show that cross-linking significantly increases the size of the LtEc nanoparticles (164 ± 13...
June 19, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Hao Dong, Guoyu Wu, Hong Xu, Chenghong Zhang, Jiao Wang, Meng Gao, Yue Pang, Houli Zhang, Baojing Zhang, Yan Tian, Qingwei Li
Primary liver cancer (PLC) is one of the most common malignant tumours and has the third highest mortality rate worldwide. An active liver-targeting drug delivery system via the asialoglycoprotein receptors expressed in the hepatic parenchyma cells of mammals has become a research focus for the treatment of PLC. N-acetylaminogalactosyl-poly(lactide-co-glycolide)-succinyl-D-α-tocopherol polyethylene glycol 1000 succinate (GalNAc-PLGA-sTPGS) was synthesized to achieve active liver-targeting properties. Emodin (EMO)-loaded GalNAc-PLGA-sTPGS nanoparticles (EGPTN) were prepared with EMO which was selected for its potential antitumour efficacy...
June 19, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Lemei Dong, Huisuo Hong, Xiaowei Chen, Zhiming Huang, Wei Wu, Fang Wu
Recently, long non-coding RNAs (lncRNAs) were involved in promoting gastric cancer (GC) initiation and progression. In the current study, we revealed that the expression level of LINC02163 was elevated in GC cell lines and tissues. Knockdown of LINC02163 inhibited GC cells growth and invasion both in vitro and in vivo. Mechanismly, LINC02163 exerted as a ceRNA and negatively regulated miR-593-3p expression. In addition, FOXK1 was identified as a down-stream target of miR-593-3p. The miR-593-3p/FOXK1 axis mediated LINC02163's effect on GC...
June 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
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