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Artificial Cells, Nanomedicine, and Biotechnology

Hamidreza Kheiri Manjili, Ali Sharafi, Elahe Attari, Hossein Danafar
A reliable and efficient drug delivery system using PCL-PEG-PCL copolymers was established for the anti-cancer compound sulforaphane (SF) in this study. Encapsulated SF by PCL-PEG-PCL nanoparticles led to formation of SF-loaded PCL-PEG-PCL micelles. Micelles characterization and stability, the particle size and their morphology were determined by DLS and AFM. The loading efficiency of SF was 19.33 ± 1.28%. The results of AFM showed that the micelles had spherical shapes with the size of 107 nm. In vitro release of SF from SF-entrapped micelles was remarkably sustained...
February 1, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Khawla S Khashan, Ghassan M Sulaiman, Rafal Mahdi
The antimicrobial activity was investigated for iron oxide IO nanoparticles (NPs)-decorated carbon nanotubes CNT prepared successfully by Nd:YAG-pulsed laser ablation in the liquid process. TEM reveals the composite NP and exhibits semispherical of iron oxide NPs, which aggregate around rolled and unrolled graphene sheet. XRD pattern proved the presence of carbon and different phases of IO NPs. Then, the antibacterial activity of the NPs was examined against different bacteria using nutrient broth and nutrient agar methods, which was enhanced using IO...
February 1, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Vratika C Sonawane, Mahesh P More, Abhijeet P Pandey, Pravin O Patil, Prashant K Deshmukh
Present investigation deals with, tacrolimus eluting, self-expandable, biodegradable stent fabricated by solvent casting method. The design was based on shape memory polymers, which possess the ability to memorize temporary shape that can substantially differ from their initial permanent shape. A set of biodegradable polymers blend was used such as poly-lactic acid (PLA) and poly-l-glycolic acid (PLGA) to study the shape memory effect of polymer. The prepared stent was assessed for various parameters like Scanning Electron Microscopy (SEM), In-vitro and Ex vivo expansion, Drug content, In-vitro drug release, Haemocompatibility, Differential Scanning Calorimetry (DSC), Fourier Transform Infrared spectroscopy (FTIR), and Textural Characterization...
January 31, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Hemali Padalia, Sumitra Chanda
Zinc oxide nanoparticles (ZnO NPs) were synthesized using Ziziphus nummularia leaf extract. The characterization was done by various spectral analysis and antifungal (anti-candidal) activity against multidrug resistant clinical isolates and their cytotoxic potential was evaluated. The ZnO NPs were 17.33 nm in size and were spherical/irregular in shape. The antifungal activity of ZnO NPs was better than four standard azole antibiotics and they also showed potent cytotoxic effect against HeLa cancer cell line...
January 31, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Gobinath Chandrakasan, PrabuKumar Seetharaman, Sathishkumar Gnanasekar, Murugan Kadarkarai, Sivaramakrishnan Sivaperumal
In this study, extracellular metabolites of symbiotic bacteria Xenorhabdus stockiae (KT835471) was employed for the synthesis of silver (XsAgNPs) and gold nanoparticles (XsAuNPs). Synthesized NPs were characterized using high throughput instrumentation which confirms the generation of stable, crystalline XsAgNPs and XsAuNPs with the mean size of 14 ± 6 and 14 ± 5, respectively. Further, the NPs exhibits an excellent bactericidal effect against six different pathogens. On the other hand, NPs displayed an outstanding anticancer activity against human lung adenocarcinoma epithelial cells (A549)...
January 31, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Jing Zhao, Chengbin Yan, Lijuan Xu, Kunping Yan, Bao Feng, Mengye Zhao, Geng Niu, Mengdi Wu, Chao Chen, Hongli Zhu
The effects of polymerized porcine hemoglobin (pPolyHb) on hemodynamic stability and maintenance of mesenteric microvascular function were explored in a rat model of hemorrhagic shock (HS). Following controlled hemorrhage, rats were infused with equal volumes of either pPolyHb, hetastarch (HES), or red blood cell (RBC). The results showed that pPolyHb was superior to HES and RBC in restoring hemodynamic stability and reversing anaerobic metabolism. We observed a reduction in the diameter of mesenteric microvasculature after HS...
January 27, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Xiufan Du, Fangli Huang, Shujiang Zhang, Yongchang Yao, Yi Chen, Yushu Chen, Hongxuan Huang, Bo Bai
OBJECTIVES: The present study aimed to develop microparticles of phenolic hydroxyl derivative of carboxymethylcellulose (CMC-Ph) via Co-flow microfluidics technology and encapsulated gene-modified rat bone mesenchymal stem cells (BMSCs) for the detection of the growth factor release was controlled by Tet-on system. Meanwhile, we investigated the effect of the CMC-Ph microcapsules and Lentiviral transduction on osteogenesis of BMP2-BMSCs. METHODS: The middle size of CMC-Ph microcapsules was prepared by optimized co-flow microfluidics through ejecting fluid CMC-Ph suspension (mixed with HRP) into co-flowing liquid paraffin which blends H2O2 at priority...
January 27, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Alireza Valizadeh, Nasrin Sohrabi, Fariba Badrzadeh
AIDS is one of the global pandemic diseases that results from infection by HIV and was estimated 34.2 million people infected in 2011 by this virus. The investigators had previously shown that by early antiretroviral treatment, the risk of AIDS/HIV-related illness and transmission reduced significantly. Nanomaterials could be applied to improving the ability and sensitivity of sensors to detect serum biomarkers with low-sample volume. Moreover, results can be obtained faster. In this paper, we present a review of several experimental studies for HIV electrochemical detection based on nanomaterials...
January 27, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Dheeraj Sharma, Manpreet Singh, Punnet Kumar, Vir Vikram, Neeraj Mishra
The aim of this study is to prepare and characterize intranasal delivery of morin hydrate loaded microemulsion for the management of Alzheimer's diseases. After intranasal delivery, brain and blood drug concentrations were found to be higher for optimized morin hydrate loaded microemulsion as compared to plain morin hydrate. Significant (P < 0.05) reduction in assessed pharmacodynamic parameters was observed after intranasal administration of morin hydrate loaded microemulsion as compared to sham control group...
January 19, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Adem Sahin, Gunes Esendagli, Firat Yerlikaya, Secil Caban-Toktas, Digdem Yoyen-Ermis, Utku Horzum, Yesim Aktas, Mansoor Khan, Patrick Couvreur, Yilmaz Capan
In this study, it was aimed to investigate characteristics and intracellular delivery of two different-sized PLGA nanoparticles in ouzo region by considering number of nanoparticles. To determine the effect of formulation parameters on average particle size, Dil labeled nanoparticles were prepared using a three-factor, two-level full factorial statistical experimental design. PLGA230 (230.8 ± 4.32 nm) and PLGA160 (157.9 ± 6.16 nm) nanoparticles were obtained by altering polymer amount based on experimental design results and characterized...
January 13, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Elaine S Teh, Franck Zal, Valérie Polard, Philippe Menasché, David J Chambers
BACKGROUND: Prior to heart transplantation, static storage of donor hearts is currently limited to 4-5 h, despite profound hypothermia (4-8 °C). Because heart transplantation is an emergency procedure, improved protection to extend safe storage duration would be advantageous. We investigated whether the naturally respiratory pigment HEMO2life(®), which is effective at hypothermia for the passive release of oxygen via oxygen gradient, could improve long-term preservation. METHODS: Isolated Langendorff-perfused rat hearts (n = 12/group) were equilibrated (20 min) and function (left ventricular developed pressure: LVDP) measured by intraventricular balloon before arrest with cold (7...
January 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Serap Durkut, Yaşar Murat Elçin
In this study, we synthesized poly(N-vinylcaprolactam)-g-collagen (PNVCL-g-Col) by grafting collagen with carboxyl group-terminated thermosensitive PNVCL-COOH. The resulting biopolymer was evaluated for its structural, thermal, and rheological properties. Aqueous solutions of PNVCL-g-Col exhibited a temperature-dependent phase transition around human physiological temperature (at ∼38.5 °C), and temperature-dependent tunability. PNVCL-g-Col exhibited temperature-dependent release of the model drugs, lidocaine hydrochloride and bovine serum albumin...
January 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Soumaye Amirsaadat, Younes Pilehvar-Soltanahmadi, Faraz Zarghami, Shahriar Alipour, Zohreh Ebrahimnezhad, Nosratollah Zarghami
Nanoparticle-based targeted drug delivery has the potential for rendering silibinin specifically at the favorite site using an external magnetic field. Also, it can circumvent the pitfalls of poor solubility. For this purpose, silibinin-loaded magnetic nanoparticles are fabricated, characterized and evaluated cytotoxicity and hTERT gene expression in A549 lung cancer cell line. silibinin-loaded PLGA-PEG-Fe3O4 had dose- and time-dependent cytotoxicity than pure silibinin. Additionally, hTERT expression is more efficiently reduced with increasing concentrations of nanosilibinin than pure silibinin...
January 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Armaghan Ghiaee, Faeze Pournaqi, Saeid Vakilian, Abdolah Mohammadi-Sangcheshmeh, Abdolreza Ardeshirylajimi
Issues associated with tissue transplantation and shortage of donors has always been a concern, whereas tissue engineering has provided the hopeful opportunities. The aim of this study was to investigate the chondrogenic differentiation potential of mesenchymal stem cells (MSCs) in the presence of embedded Dexamethasone into electrospun Poly(ethyleneoxide) nanofibers composited with Poly(ɛ-caprolactone) nanofibers. Electrospun-fabricated scaffolds were characterized by SEM, tensile, contact angle, release profile, MTT assay, and chonderogenic differentiation of stem cells...
January 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Supandeep Singh Hallan, Veerpal Kaur, Vikas Jain, Neeraj Mishra
The present study aimed to develop an improved oral delivery system for low-molecular-weight heparin (LMWH), novel polymer lipid hybrid nanoparticles were developed. LMWH loaded chitosan polymer lipid hybrid nanoparticles (LMWH-CS-PLNs) were developed using double emulsification and solvent evaporation method. The performance of developed formulations was evaluated by using in vitro and in vivo behavior, such as drug release studies, in vitro permeation study, in vivo venous thrombolytic study, in vitro uptake studies by using intestinal epithelium resembling Caco-2 cell lines...
January 10, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Rajendiran Ramkumar, Govindasamy Balasubramani, Ramalingam Karthik Raja, Manickam Raja, Raji Govindan, Easwaradas Kreedapathy Girija, Pachiappan Perumal
The Lantana camara Linn root extract derived gold nanoparticles (Au NPs) were characterized by Ultraviolet-Visible spectroscopy, X-ray diffraction, fourier transform-infrared, high resolution transmission electron microscopy, selected area electron diffraction pattern and energy dispersive X-ray analyses. In DPPH assay, the inhibitory concentration (IC50) of Au NPs and gallic acid was 24.17 and 5.39 μg/ml, whereas, for cytotoxicity assay, the IC50 of Au NPs was 17.72 and 32.98 μg/ml on MBA-MB-231 and Vero cells, respectively...
January 9, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Amir Mehdizadeh, Mohammad Hossein Somi, Masoud Darabi, Safar Farajnia, Abolfazl Akbarzadeh, Soheila Montazersaheb, Mehdi Yousefi, Mortaza Bonyadi
BACKGROUND: Extracellular signal-regulated kinase (Erk)1 and Erk2 are central mediators of mitogen-activated protein kinase signaling pathway, which plays a key role in proliferation and chemoresistance of cancer cells. However, the effect of Erk1 and Erk2 in these processes may not be the same. The aim of this study was to investigate differential effect of Erk1 and Erk2 down-regulation on chemoresistance in human hepatocellular carcinoma (HCC) cells. Expression level and relative expression analysis in HepG2 cells were performed using RT-PCR and qRT-PCR, respectively...
January 6, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Manmohan Singh Jangdey, Anshita Gupta, Shailendra Saraf, Swarnlata Saraf
The aim of this work is to apply Box-Behnken design to optimize the transfersomes were formulated by modified rotary evaporation sonication technique using surfactant Tween 80. The response surface methodology was used having three-factored with three levels. The prepared formulations were characterized for vesicle shape, size, entrapment efficiency (%), stability, and in vitro permeation. The result showed that drug entrapment of 84.24% with average vesicle size of 35.41 nm and drug loading of 8.042%. Thus, optimized formulation was found good stability and is a promising approach to improve the permeability of apigenin in sustained release for prolonged period of time...
January 4, 2017: Artificial Cells, Nanomedicine, and Biotechnology
Ruo-Wang Duan, Jiong Song, Yu-Ping Li, Chun-Gen Xing
The objective of this work was to develop a simple, selective, and sensitive LC-MS/MS method for the quantitation of the mepivacaine in Chinese biological matrix. The calibration curve of mepivacaine ranged from 0.5 to 2000 ng/mL with the lower limit of quantitation being 0.5 ng/mL. This sensitivity was high enough to describe the profile of blood mepivacaine level versus time. Thereby it was very desirable for the pharmacokinetic study because of its high sensitivity and accuracy. The study used a single-dose two-period crossover design principle...
December 15, 2016: Artificial Cells, Nanomedicine, and Biotechnology
Sepideh Hamedi, Masumeh Ghaseminezhad, Soheila Shokrollahzadeh, Seyed Abbas Shojaosadati
The controlled synthesis of silver nanoparticles (AgNPs) using cell-free filtrate of Fusarium oxysporum fungus was investigated. The effect of fungal incubation period on nanoparticle formation and nitrate reductase enzyme activity was studied using UV-visible spectroscopy and Harley assay, respectively. The highest AgNP formation was observed in the cell-free filtrate of biomass harvested at the early stationary phase where the NR enzyme activity is the maximum. Mixing of the cell-free filtrates of fungal cultures obtained at 23, 28, and 33 °C with silver nitrate solution confirms the higher productivity of AgNP biosynthesis using the cell-free filtrate of fungus incubated at 28 °C...
December 14, 2016: Artificial Cells, Nanomedicine, and Biotechnology
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