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RSC Advances

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https://www.readbyqxmd.com/read/28670448/a-novel-application-of-the-staudinger-ligation-to-access-neutral-cyclic-di-nucleotide-analog-precursors-via-a-divergent-method
#1
M H Fletcher, C E Burns-Lynch, K W Knouse, L T Abraham, C W DeBrosse, W M Wuest
Our efforts to develop a scalable and divergent synthesis of cyclic di-nucleotide analog precursors have resulted in (1) an orthogonally protected di-amino carbohydrate as well as (2) the novel application of the Staudinger ligation to provide medium-sized macrocycles featuring carbamate or urea linkages.
June 7, 2017: RSC Advances
https://www.readbyqxmd.com/read/28670447/short-term-hypoxic-preconditioning-promotes-prevascularization-in-3d-bioprinted-bone-constructs-with-stromal-vascular-fraction-derived-cells
#2
Mitchell A Kuss, Robert Harms, Shaohua Wu, Ying Wang, Jason B Untrauer, Mark A Carlson, Bin Duan
Reconstruction of complex, craniofacial bone defects often requires autogenous vascularized bone grafts, and still remains a challenge today. In order to address this issue, we isolated the stromal vascular fraction (SVF) from adipose tissues and maintained the phenotypes and the growth of endothelial lineage cells within SVF derived cells (SVFC) by incorporating an endothelial cell medium. We 3D bioprinted SVFC within our hydrogel bioinks and conditioned the constructs in either normoxia or hypoxia. We found that short-term hypoxic conditioning promoted vascularization-related gene expression, whereas long-term hypoxia impaired cell viability and vascularization...
June 5, 2017: RSC Advances
https://www.readbyqxmd.com/read/28580138/plasma-membrane-association-facilitates-conformational-changes-in-the-marburg-virus-protein-vp40-dimer
#3
Nisha Bhattarai, Jeevan B Gc, Bernard S Gerstman, Robert V Stahelin, Prem P Chapagain
Filovirus infections cause hemorrhagic fever in humans and non-human primates that often results in high fatality rates. The Marburg virus is a lipid-enveloped virus from the Filoviridae family and is closely related to the Ebola virus. The viral matrix layer underneath the lipid envelope is formed by the matrix protein VP40 (VP40), which is also involved in other functions during the viral life-cycle. As in the Ebola virus VP40 (eVP40), the recently determined X-ray crystal structure of the Marburg virus VP40 (mVP40) features loops containing cationic residues that form a lipid binding basic patch...
April 26, 2017: RSC Advances
https://www.readbyqxmd.com/read/28603608/carbonic-acid-monoethyl-ester-as-a-pure-solid-and-its-conformational-isomerism-in-the-gas-phase
#4
Jürgen Bernard, Eva-Maria Köck, Roland G Huber, Klaus R Liedl, Ludwig Call, Robert Schlögl, Hinrich Grothe, Thomas Loerting
The monoesters of carbonic acid are deemed to be unstable and decompose to alcohol and carbon dioxide. In spite of this, we here report the isolation of the elusive carbonic acid monoethyl ester (CAEE) as a pure solid from ethanolic solutions of potassium bicarbonate. The hemiester is surprisingly stable in acidic solution and does not experience hydrolysis to carbonic acid. Furthermore, it is also stable in the gas phase, which we demonstrate by subliming the hemiester without decomposition. This could not be achieved in the past for any hemiester of carbonic acid...
April 21, 2017: RSC Advances
https://www.readbyqxmd.com/read/28603607/co-delivery-of-carboplatin-and-paclitaxel-via-cross-linked-multilamellar-liposomes-for-ovarian-cancer-treatment
#5
Xiaoyang Zhang, Yarong Liu, Yu Jeong Kim, John Mac, Rachel Zhuang, Pin Wang
Carboplatin (CPT) and paclitaxel (PTX) used in combination is one of the most effective treatments for ovarian cancer. However, the traditional combination methods used to co-administrate CPT and PTX showed limited clinical efficacy due to their distinct pharmacokinetics. Although much effort has been devoted to developing nanoparticles capable of encapsulating drugs with different lipophilicites, co-delivery of carboplatin with paclitaxel by a single nanoparticle has rarely been reported. Here, we encapsulated and delivered this drug combination to ovarian cancer cells at a controlled ratio by a previously reported crosslinked multilamellar liposome vesicle (cMLV)...
April 3, 2017: RSC Advances
https://www.readbyqxmd.com/read/28603606/synthesis-and-properties-of-magnetic-optical-core-shell-nanoparticles
#6
Elyahb Allie Kwizera, Elise Chaffin, Yongmei Wang, Xiaohua Huang
Due to their high integrity, facile surface chemistry, excellent stability, and dual properties from the core and shell materials, magnetic-plasmonic core-shell nanoparticles are of great interest across a number of science, engineering and biomedical disciplines. They are promising for applications in a broad range of areas including catalysis, energy conversion, biological separation, medical imaging, disease detection and treatment. The technological applications have driven the need for high quality nanoparticles with well controlled magnetic and optical properties...
March 19, 2017: RSC Advances
https://www.readbyqxmd.com/read/28515901/experimental-identification-and-computational-characterization-of-a-novel-extracellular-metalloproteinase-produced-by-clostridium-sordellii
#7
Michael J Aldape, Aoxiang Tao, Dustin D Heeney, Eric R McIndoo, John M French, Dong Xu
Clostridium sordellii is a lethal pathogen for both animals and humans. Severe capillary leakage, toxic shock syndrome, and an extreme leukemoid reaction (LR), are hallmark features of C. sordellii infections and contribute to its high mortality rate. Here we report the discovery of a previously unknown and uncharacterized metalloproteinase of C. sordellii (referred as Mcs1) that cleaves human vascular cell adhesion molecule (VCAM)-1 in vitro, an adhesion molecule critical to hematopoietic precursor retention and leukocyte diapedesis...
March 2, 2017: RSC Advances
https://www.readbyqxmd.com/read/28496973/8-styryl-substituted-coralyne-derivatives-as-dna-binding-fluorescent-probes
#8
P M Pithan, D Decker, S I Druzhinin, H Ihmels, H Schönherr, Y Voß
Six new 8-styryl-substituted coralyne derivatives 4a-f were synthesized from coralyne (2) by a base catalysed Knoevenagel type reaction. It was shown by photometric and fluorimetric titrations of double stranded and quadruplex DNA to 4b-d as well as by fluorimetric DNA denaturation experiments that these ligands bind to DNA with different binding modes at varying ligand-DNA ratios (LDR). Specifically, the addition of DNA caused initially a hypochromic effect in absorbance and, at a particular LDR, the development of a new red shifted absorption band with a hyperchromic effect...
February 8, 2017: RSC Advances
https://www.readbyqxmd.com/read/28496972/vuv-uv-light-inducing-accelerated-phenol-degradation-with-a-low-electric-input
#9
Mengkai Li, Dong Wen, Zhimin Qiang, John Kiwi
This study presents the first evidence for the accelerated degradation of phenol by Fenton's reagent in a mini-fluidic VUV/UV photoreaction system (MVPS). A low-pressure mercury lamp used in the MVPS led to a complete degradation of phenol within 4-6 min. The HO˙ and HO2˙ originating from both Fenton's reagent and VUV photolysis of water were identified with suitable radical scavengers. The effects of initial concentrations of phenol, H2O2 and Fe(3+) as well as solution pH on phenol degradation kinetics were examined...
January 23, 2017: RSC Advances
https://www.readbyqxmd.com/read/28496976/membrane-properties-and-anti-bacterial-anti-biofouling-activity-of-polysulfone-graphene-oxide-composite-membranes-phase-inversed-in-graphene-oxide-non-solvent
#10
V R S S Mokkapati, Derya Yuksel Koseoglu-Imer, Nurmiray Yilmaz-Deveci, Ivan Mijakovic, Ismail Koyuncu
A new and facile method for the fabrication of polysulfone-graphene oxide composite membranes is reported, where after casting, phase inversion is carried out with graphene oxide flakes (GO) in a coagulation bath. The membranes were characterized and the morphology was analysed using scanning electron microscopy. A bacterial inhibition ratio of 74.5% was observed with membranes fabricated from a very low concentration of di-water-GO non-solvent (0.048% of GO). The membranes were successfully tested for permeate flux and fouling resistance using activated sludge filtration from an MBR system...
January 16, 2017: RSC Advances
https://www.readbyqxmd.com/read/28496975/convergent-synthesis-and-optical-properties-of-near-infrared-emitting-bioluminescent-infra-luciferins
#11
James C Anderson, Helen Grounds, Amit P Jathoul, James A H Murray, Steven J Pacman, Laurence Tisi
Infra-luciferin, an alkene linked analogue of luciferin, gives bioluminescence emission >700 nm and has the potential to be used for multiparametric in vivo imaging. We report here a high yielding, scalable and convergent synthesis of infra-luciferin which will allow the synthesis of other conjugated luciferins for investigation in near-infrared bioluminescence imaging. We demonstrated this potential by using the new route to synthesise a diene linked analogue of luciferin, the fluorescent and bioluminescent properties of which were compared to those of d-luciferin and infra-luciferin...
January 16, 2017: RSC Advances
https://www.readbyqxmd.com/read/28496974/computational-analysis-of-the-solvation-of-coffee-ingredients-in-aqueous-ionic-liquid-mixtures
#12
Veronika Zeindlhofer, Diana Khlan, Katharina Bica, Christian Schröder
In this paper, we investigate the solvation of coffee ingredients including caffeine, gallic acid as representative for phenolic compounds and quercetin as representative for flavonoids in aqueous mixtures of the ionic liquid 1-ethyl-3-methylimidazolium acetate [C2mim][OAc] at various concentrations. Due to the anisotropy of the solutes we show that classical Kirkwood-Buff theory is not appropriate to study solvation effects with increasing ionic liquid content. However, excess coordination numbers as well as the mean residence time of solvent molecules at the surface of the solutes can be determined by Voronoi tessellation...
January 13, 2017: RSC Advances
https://www.readbyqxmd.com/read/29104751/conformation-dynamics-of-the-intrinsically-disordered-protein-c-myb-with-the-ff99idps-force-field
#13
Xiang Guo, Jincheng Han, Ray Luo, Hai-Feng Chen
The intrinsically disordered protein c-Myb plays a critical role in cellular proliferation and differentiation. Loss of c-myb function results in embryonic lethality due to failure of fetal hepatic hematopoiesis. The conformation dynamics of the intrinsically disordered c-Myb are still unknown. Here, molecular dynamics (MD) simulations with the intrinsically disordered protein force field ff99IDPs were used to study the conformation dynamics. In comparison with ff99SBildn, ff99IDPs can reproduce more diverse disordered conformers of c-Myb...
2017: RSC Advances
https://www.readbyqxmd.com/read/28989703/an-amphipathic-trans-acting-phosphorothioate-rna-element-delivers-an-uncharged-phosphorodiamidate-morpholino-sequence-in-mdx-mouse-myotubes
#14
H V Jain, J F Boehler, D Verthelyi, K Nagaraju, S L Beaucage
An efficient method for the delivery of uncharged polyA-tailed phosphorodiamidate morpholino sequences (PMO) in mammalian cells consists of employing a synthetic 8-mer amphipathic trans-acting poly-2'-O-methyluridylic thiophosphate triester element (2'-OMeUtaPS) as a transfection reagent. Unlike the dTtaPS DNA-based element, this RNA element is potent at delivering polyA-tailed PMO sequences to HeLa pLuc 705 cells or to myotube muscle cells. However, much like dTtaPS, the 2'-OMeUtaPS-mediated internalization of PMO sequences occurs through an energy-dependent mechanism; macropinocytosis appears to be the predominant endocytic pathway used for cellular uptake...
2017: RSC Advances
https://www.readbyqxmd.com/read/28989702/selective-adhesive-cell-capture-without-molecular-specificity-new-surfaces-exploiting-nanoscopic-polycationic-features-as-discrete-adhesive-units
#15
S Kalasin, E P Browne, K F Arcaro, M M Santore
This work explored how molecularly non-specific polycationic nanoscale features on a collecting surface control kinetic and selectivity aspects of mammalian cell capture. Key principles for selective collector design were demonstrated by comparing the capture of two closely related breast cancer cell lines: MCF-7 and TMX2-28. TMX2-28 is a tamoxifen-selected clone of MCF-7. The collector was a silica surface, negatively-charged at pH 7.4, containing isolated molecules (~ 8 nm diameter) of the cationic polymer, poly(dimethyl-aminoethylmethacrylate), pDMAEMA...
2017: RSC Advances
https://www.readbyqxmd.com/read/28966788/identification-of-a-fragmented-small-gtpase-capable-of-conditional-effector-binding
#16
Jia Zhao, Cliff I Stains
A fragmented small GTPase capable of conditional effector binding is described. The effector binding function of this split-GTPase can be modulated using a small molecule input, thus allowing for the potential design of cellular signaling pathways.
2017: RSC Advances
https://www.readbyqxmd.com/read/28944051/expanding-analytical-tools-for-characterizing-ultrasmall-silica-based-nanoparticles
#17
B Yoo, K Ma, U Wiesner, M Bradbury
C' dots are fluorescent inorganic-organic hybrid nanoparticles synthesized in water comprised of a silica core with a covalently embedded near infrared dye, and a polyethylene glycol (PEG) outer layer. C' dots containing the integrin specific ligand, cycloRGDyC, are the first of their kind particles approved for human clinical trials. In the continued clinical development of these nanoparticles, high-resolution analytical approaches are needed. Here we investigate the use of reversed phase high performance liquid chromatography (RP-HPLC) to analyze cycloRGDyC-Cy5-C' dots...
2017: RSC Advances
https://www.readbyqxmd.com/read/28944050/carbohydrates-as-potentially-versatile-core-subcarriers-for-multivalent-immunogens
#18
Divya Kushwaha, Peng Xu, Pavol Kováč
Synthetic multivalent glycoclusters that carry carbohydrate antigen epitopes have been recognized as promising candidates for the development of carbohydrate based vaccines. Here we describe a convergent strategy for the synthesis of conjugation-ready multivalent glycoclusters using sugars as versatile core subcarriers. d-Glucose and gentiobiose were converted into poly-alkyne functionalized cores which were then decorated with an azide bearing model ligand d-glucose using click chemistry, to form structurally well-defined tetra- and heptavalent glycoclusters...
2017: RSC Advances
https://www.readbyqxmd.com/read/28936355/theoretical-and-experimental-study-of-the-antifreeze-protein-afp752-trehalose-and-dimethyl-sulfoxide-cryoprotection-mechanism-correlation-with-cryopreserved-cell-viability
#19
Irena Kratochvílová, Martin Golan, Karel Pomeisl, Jan Richter, Silvia Sedláková, Jakub Šebera, Júlia Mičová, Martin Falk, Iva Falková, David Řeha, K Wade Elliott, Krisztina Varga, Shelby E Follett, Daniel Šimek
In this work the physico-chemical properties of selected cryoprotectants (antifreeze protein TrxA-AFP752, trehalose and dimethyl sulfoxide) were correlated with their impact on the constitution of ice and influence on frozen/thawed cell viability. The freezing processes and states of investigated materials solutions were described and explained from a fundamental point of view using ab-initio modelling (molecular dynamics, DFT), Raman spectroscopy, Differential Scanning Calorimetry and X-Ray Diffraction. For the first time, in this work we correlated the microscopic view (modelling) with the description of the frozen solution states and put these results in the context of human skin fibroblast viability after freezing and thawing...
2017: RSC Advances
https://www.readbyqxmd.com/read/28603605/phase-behavior-of-mixed-lipid-monolayers-on-perfluorocarbon-nanoemulsions-and-its-effect-on-acoustic-contrast
#20
Rajarshi Chattaraj, Galen M Goldscheitter, Adem Yildirim, Andrew P Goodwin
Lipid-stabilized nanoemulsions containing a volatile liquid perfluorocarbon core have been studied as ultrasound contrast agents owing to their ability to transform into high-contrast microbubbles when subjected to high intensity focused ultrasound (HIFU). However, while there have been several studies on the effect of acoustic parameters on contrast, the effect of the droplet's stabilizing shell has not been studied as extensively. Inspired by previous studies showing lateral phase separation in microbubbles and vesicles, nanodroplets were formulated with a perfluorohexane core and a shell composed of varying amounts of saturated (DPPC) phospholipids, unsaturated (DOPC) phospholipids, and cholesterol, which were fractionated to obtain nanodroplets of mean diameter 300-400 nm and were stable over one week...
December 4, 2016: RSC Advances
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