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Bioengineered

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https://www.readbyqxmd.com/read/27893301/optimizing-the-expression-of-a-heterologous-chitinase-a-study-of-different-promoters
#1
Abigail F da Silva, Belén García-Fraga, Jacobo López-Seijas, Carmen Sieiro
Many relevant applications have been demonstrated for chitinolytic enzymes. However, their successful exploitation depends upon the availability of strains and expression conditions that allow the production of active forms and large quantities of these enzymes. Escherichia coli has been commonly used to express and overproduce different proteins, among them chitinases. Improving the functional gene expression of chitinases is key to exploiting their potential. In a recent study, we described the effect of various parameters on the functional expression of 2 chitinases from different families, demonstrating that the effect of each of these parameters on the activity of both chitinases was specific to each enzyme...
November 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27849134/developing-and-characterization-of-single-chain-variable-fragment-scfv-antibody-against-frizzled-7-fzd7-receptor
#2
Hamid Nickho, Vahid Younesi, Leili Aghebati-Maleki, Morteza Motallebnezhad, Jafar Majidi Zolbanin, Aliakbar Movassagh Pour, Mehdi Yousefi
Wnt/β-catenin signaling pathway through Frizzled receptors has been shown to play a key role in both normal development and tumorigenesis. Overexpression of Wnt pathway genes, such as Fzd7 in several malignancies is well-documented. Therefore, targeting of Fzd7 and its ligand inhibits cancer cells proliferation metastasis. In the present study we isolated single chain variable fragments (scFvs) against Fzd7 receptor using phage display method. Semi-synthetic human naive antibody libraries (Tomlinson I + J) was employed in panning procedure to isolate specific scFv against specific peptide from extracellular domain of Fzd7 receptor...
November 16, 2016: Bioengineered
https://www.readbyqxmd.com/read/27813700/current-challenges-for-the-targeted-delivery-and-molecular-imaging-of-stem-cells-in-animal-models
#3
Arezoo Momeni, Sriram Neelamegham, Natesh Parashurama
In contrast to conventional, molecular medicine that focuses on targeting specific pathways, stem cell therapy aims to perturb many related mechanisms in order to derive therapeutic benefit. This emerging modality is inherently complex due to the variety of cell types that can be used, delivery approaches that need to be optimized in order to target the cellular therapeutic to specific sites in vivo, and non-invasive imaging methods that are needed to monitor cell fate. This review highlights advancements in the field, with focus on recent publications that use preclinical animal models for cardiovascular stem cell therapy...
November 4, 2016: Bioengineered
https://www.readbyqxmd.com/read/27791465/production-of-encapsulated-creatinase-using-yeast-spores
#4
Jun Kong, Zijie Li, Huijie Zhang, Xiao-Dong Gao, Hideki Nakanishi
Yeast spores can be used as a carrier to produce enzyme capsules. In the present study, this technique was applied to a diagnostic enzyme named creatinase. We found that a secretory form of Pseudomonas putida creatinase could be entrapped in the spore wall, and such spores were used as creatinase capsules. The activity of the encapsulated creatinase was largely improved by mild spore wall defective mutations, such as DIT1 or OSW2 deletions. The advantages of this method include the following: encapsulated and freeze-dried creatinase is produced without preparing the purified enzyme, and it exhibits resistance to environmental stresses, such as high temperature and SDS treatments...
October 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27791460/effect-of-testosterone-on-the-proliferation-and-collagen-synthesis-of-cardiac-fibroblasts-induced-by-angiotensin-ii-in-neonatal-rat
#5
Xiaocun Yang, Ying Wang, Shuxun Yan, Lina Sun, Guojie Yang, Yuan Li, Chaonan Yu
The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone group, and testosterone + Ang II group in vitro. Cell cycle distribution, collagen counts, and phosphorylated extracellular signal-regulated kinase (ERK1/2) (p - ERK1/2) expression were assessed by flow cytometry, VG staining, and immunocytochemistry, respectively...
October 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27791456/ex-vivo-comparative-study-on-three-sinus-lift-tools-for-transcrestal-detaching-maxillary-sinus-mucosa
#6
Yanfeng Li, Pin Hu, Yishi Han, Jiadong Fan, Xinming Dong, Huan Ren, Chunhao Yang, Tingting Shi, Dong Xia
OBJECTIVE: The objective of this study was to comparatively evaluate 3 different sinus lift tools, namely umbrella-shaped sinus lift curette YSL-04, our recently designed probe-improved sinus lift curettes, and our newly invented elevator 014, using our previous developed goat ex vivo models for direct visualizing the effectiveness of detaching sinus mucosa in real time. METHODS: Goat ex vivo models for direct visualizing the effectiveness of detaching sinus mucosa in real time were generated according to our previously developed protocol...
October 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27791455/in-vitro-and-in-silico-interaction-of-porcine-%C3%AE-amylase-with-vicia-faba-crude-seed-extract-and-evaluation-of-antidiabetic-activity
#7
Dhiraj Kumar Choudhary, Abha Mishra
Diabetes mellitus is a group of metabolic disorder of glucose metabolism. The proper management of blood glucose level is an indicator in the treatment of this complex pathology. The aim of the present study was to evaluate the inhibitory potential of Vicia faba crude seed extracts on the activities of α-amylase. Phytochemical screening, FTIR and HPLC analysis confirmed the presence of a phenolic compound with percentage yield of 9.87% in the acetone extract. Acetone extract of seed had highest inhibitory potential against porcine α-amylase (IC50 value of 2...
October 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27791453/new-biotechnological-applications-for-ashbya-gossypii-challenges-and-perspectives
#8
Tatiana Q Aguiar, Rui Silva, Lucília Domingues
The filamentous fungus Ashbya gossypii has long been considered a paradigm of the White Biotechnology in what concerns riboflavin production. Its industrial relevance led to the development of a significant molecular and in silico modeling toolbox for its manipulation. This, together with the increasing knowledge of its genome and metabolism has helped designing effective metabolic engineering strategies for optimizing riboflavin production, but also for developing new A. gossypii strains for novel biotechnological applications, such as production of recombinant proteins, single cell oils (SCOs), and flavour compounds...
October 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27791452/the-growing-impact-of-lyophilized-cell-free-protein-expression-systems
#9
J Porter Hunt, Seung Ook Yang, Kristen M Wilding, Bradley C Bundy
Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not only overcome cold-storage limitations, but also enable stockpiling for on-demand synthesis and completely sterilize the protein synthesis platform. Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis...
October 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27786573/effect-and-mechanism-of-calpains-on-pediatric-lobar-pneumonia
#10
Genquan Yin, Qiang Zeng, Haijin Zhao, Peiqiong Wu, Shaoxi Cai, Li Deng, Wenhui Jiang
BACKGROUND: Lobar pneumonia, one of the community-acquired pneumonia (CAP), is a common pediatric low respiratory tract infection. Calpains are Ca(2+)-activated cysteine proteases whose activation mechanism is elusive. The present study was undertaken to detect the role and mechanism of calpains in pediatric lobar pneumonia. MATERIAL AND METHODS: The human acute lung infection model (ALIM) was constructed and infected by Streptococcus. Enzyme-linked immunosorbent assay (ELISA) was used to measure interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α...
October 27, 2016: Bioengineered
https://www.readbyqxmd.com/read/27780405/optimization-of-physical-and-morphological-regime-for-improved-cellulase-free-xylanase-production-by-fed-batch-fermentation-using-aspergillus-niger-kp874102-1-and-its-application-in-bio-bleaching
#11
Uma Shankar Prasad Uday, Tarun Kanti Bandyopadhyay, Saswata Goswami, Biswanath Bhunia
The physiological and morphological changes were extensively studied during fed batch fermentation using newly isolated Aspergillus niger (KP874102.1). Significantly higher xylanase production was possible through optimization of environmental stresses by fed batch process. The fed batch fermentation was carried out for improved xylanase production (2524 U) where initial xylan was kept 1.5 g/L in the production medium. However, 3 g/L of xylan with 50 mM K2HPO4 having pH-7 was consecutively fed at 72 and 120 h of fermentation...
October 26, 2016: Bioengineered
https://www.readbyqxmd.com/read/27780398/microbial-cooperation-in-the-rhizosphere-improves-liquorice-growth-under-salt-stress
#12
Dilfuza Egamberdieva, Stephan Wirth, Li Li, Elsayed Fathi Abd-Allah, Kristina Lindström
Liquorice (Glycyrrhiza uralensis Fisch.) is one of the most widely used plants in food production, and it can also be used as an herbal medicine or for reclamation of salt-affected soils. Under salt stress, inhibition of plant growth, nutrient acquisition and symbiotic interactions between the medicinal legume liquorice and rhizobia have been observed. We recently evaluated the interactions between rhizobia and root-colonizing Pseudomonas in liquorice grown in potting soil and observed increased plant biomass, nodule numbers and nitrogen content after combined inoculation compared to plants inoculated with Mesorhizobium alone...
October 26, 2016: Bioengineered
https://www.readbyqxmd.com/read/27775502/a-study-on-detection-of-glucose-concentration-using-changes-in-color-coordinates
#13
Ji-Sun Kim, Han-Byeol Oh, A-Hee Kim, Jun-Sik Kim, Eun-Suk Lee, Jin-Young Baek, Ki Sung Lee, Soon-Cheol Chung, Jae-Hoon Jun
Glucose concentration is closely related to the metabolic activity of cells and it is the most important substance as the energy source of a living body which plays an important role in the human body. This paper proposes an optical method that can measure the concentration of glucose. The change in glucose concentration was observed by using CIE diagram, and wavelength and purity values were detected. Also, even small changes in glucose concentration can be evaluated through mathematical modeling. This system is simple, economical, and capable of quantifying optical signals with numerical values for glucose sensing...
October 24, 2016: Bioengineered
https://www.readbyqxmd.com/read/27764542/optic-cup-segmentation-from-fundus-images-for-glaucoma-diagnosis
#14
Man Hu, Chenghao Zhu, Xiaoxing Li, Yongli Xu
Glaucoma is a serious disease that can cause complete, permanent blindness, and its early diagnosis is very difficult. In recent years, computer-aided screening and diagnosis of glaucoma has made considerable progress. The optic cup segmentation from fundus images is an extremely important part for the computer-aided screening and diagnosis of glaucoma. This paper presented an automatic optic cup segmentation method that used both color difference information and vessel bends information from fundus images to determine the optic cup boundary...
October 20, 2016: Bioengineered
https://www.readbyqxmd.com/read/27759485/synthesis-and-biological-activity-of-the-novel-indanedione-anticoagulant-rodenticides-containing-fluorine
#15
Feng Chen, Liping Liu, Zengguo Bai, Tianhua Zhang, Keke Zhao
Here, 3 fluorinated intermediates of drug were synthesized: (M1), (M2), (M3). Three new anticoagulant rodenticides were designed which were based on 4-hydroxycoumarin or 1,3-indandione, added acute toxicity groups containing fluorine. The structures of synthesized compounds were analyzed and proved by FT-IR spectroscopy and (1)H nuclear magnetic resonance ((1)H-NMR). The compounds were also evaluated for their anticoagulant and acute biologic activity. In addition, both the acute orally toxicity and the feeding indexes of R1 and R2 were tested...
October 19, 2016: Bioengineered
https://www.readbyqxmd.com/read/27754792/n-terminal-engineering-of-glutamyl-trna-reductase-with-positive-charge-arginine-to-increase-5-aminolevulinic-acid-biosynthesis
#16
Junli Zhang, Huanjiao Weng, Wenwen Ding, Zhen Kang
Five-Aminolevulinic acid (ALA), the universal precursor of all tetrapyrroles, has various applications in medicine and agriculture industries. Glutamyl-tRNA reductase (GluTR) as the first key enzyme of C5 pathway is feedback regulated by heme, and its N-terminus plays a critical role on its stability control. Here, the GluTR N-terminus was engineered by inserting different numbers of positively charged lysine and arginine residues. The results confirmed that insertion of lysine or arginine residues (especially one arginine residue) behind Thr2 significantly increased the stability of GluTR...
October 18, 2016: Bioengineered
https://www.readbyqxmd.com/read/27754760/combining-mpdl3280a-with-adoptive-cell-immunotherapy-exerts-better-antitumor-effects-against-cervical-cancer
#17
Yi Zheng, Yicheng Yang, Shu Wu, Yongqiang Zhu, Xiaolong Tang, Xiaopeng Liu
As the second most common gynecologic malignant tumors with a high mortality rate, cervical cancer jeopardizes women's life worldwide. The low cure rate in cervical cancer patients is mainly attributed to the lack of effective therapies. One feasible novel strategy is to develop immune-based approaches such as adoptive cell immunotherapy of DCCIKs which represents a promising nontoxic antineoplastic immunotherapy preferred in clinic practice. However, the therapeutic effect is not as efficient as anticipated...
October 18, 2016: Bioengineered
https://www.readbyqxmd.com/read/27753534/new-member-of-the-hormone-sensitive-lipase-family-from-the-permafrost-microbial-community
#18
Lada E Petrovskaya, Ksenia A Novototskaya-Vlasova, Sultan Sh Gapizov, Elena V Spirina, Ekaterina V Durdenko, Elizaveta M Rivkina
Siberian permafrost is a unique environment inhabited with diverse groups of microorganisms. Among them, there are numerous producers of biotechnologically relevant enzymes including lipases and esterases. Recently, we have constructed a metagenomic library from a permafrost sample and identified in it several genes coding for potential lipolytic enzymes. In the current work, properties of the recombinant esterases obtained from this library are compared with the previously characterized lipase from Psychrobacter cryohalolentis and other representatives of the hormone-sensitive lipase family...
October 18, 2016: Bioengineered
https://www.readbyqxmd.com/read/27753530/friction-properties-of-biological-functional-materials-pvdf-membranes
#19
Long Chen, Changan Di, Xuguang Chen, Zhengzhi Li, Jia Luo
Touch is produced by sensations that include approaching, sliding, pressing, and temperature. This concept has become a target of research in biotechnology, especially in the field of bionic biology. This study measured sliding and pressing with traditional tactile sensors in order to improve a machine operator's judgment of surface roughness. Based on the theory of acoustic emission, this study combined polyvinylidene fluoride (PVDF) with a sonic transducer to produce tactile sensors that can detect surface roughness...
October 18, 2016: Bioengineered
https://www.readbyqxmd.com/read/27753513/inhibition-of-the-ubiquitin-ligase-activity-improves-the-production-of-biologically-active-fusion-protein-hsa-hgf-in-chinese-hamster-ovary-cells
#20
Dongsheng Xu, Aini Wan, Jingjing Zhang, Lin Peng, Yun Chen, Yang He, Jianfeng Yang, Jian Jin
Hepatocyte growth factor (HGF) is a potent multi-functional protein that stimulates proliferation, survival, motility, scattering and differentiation during growth and development, and has been considered to be a potential therapeutic agent for the treatment of a number of intractable diseases. The aim of this study was to enhance the expression of recombinant fusion protein HSA-HGF (R494E) in CHO cells by inhibiting the intracellular ubiquitin ligase activity. The high stable expression sub-clones with different signal peptides were selected by western blot (WB) analysis and used for suspension culture...
October 18, 2016: Bioengineered
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