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Bioengineered

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https://www.readbyqxmd.com/read/28708015/relationship-between-ppar%C3%AE-mrna-expression-and-mitochondrial-respiratory-function-and-ultrastructure-of-the-skeletal-muscle-of-patients-with-copd
#1
Jian-Qing Zhang, Xiang-Yu Long, Yu Xie, Zhi-Huan Zhao, Li-Zhou Fang, Ling Liu, Wei-Ping Fu, Jing-Kui Shu, Jiang-Hai Wu, Lu-Ming Dai
Peripheral muscle dysfunction is an important complication in patients with chronic obstructive pulmonary disease (COPD). The objective of this study was to explore the relationship between the levels of peroxisome proliferator-activated receptor α (PPARα) mRNA expression and the respiratory function and ultrastructure of mitochondria in the vastus lateralis of patients with COPD. Vastus lateralis biopsies were performed on 14 patients with COPD and 6 control subjects with normal lung function. PPARα mRNA levels in the muscle tissue were detected by real-time PCR...
July 14, 2017: Bioengineered
https://www.readbyqxmd.com/read/28696140/regulatory-roles-of-24-epibrassinolide-in-tolerance-of-acacia-gerrardii-benth-to-salt-stress
#2
Elsayed Fathi Abd Allah, A A Alqarawi, Abeer Hashem, Stephan Wirth, Dilfuza Egamberdieva
This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the negative effect considerably. NaCl reduced chlorophyll content but this was significantly ameliorated by the application of EBL. EBL reduced significantly NaCl-induced oxidative stress hence protect membranes and also improved the relative water content significantly by 6...
July 11, 2017: Bioengineered
https://www.readbyqxmd.com/read/28692329/surrogate-strains-of-human-pathogens-for-field-release
#3
Sangjin Park, Chang-Hwan Kim, Seong Tae Jeong, Sang Yup Lee
Surrogate microorganisms, in short surrogates, are an essential part of pathogen research. Compared to surrogates used in controlled laboratory environments, surrogates for field release are restricted by concerns about human and environmental safety. For field research of food-borne pathogens, strains of an attenuated pathogen or strains of genetically close non-pathogenic species have been used as surrogates. Genetic modification is usually performed to attenuate virulence, through for examples deletion of genes of virulence and transcriptional regulators and removal of virulence plasmids, and to facilitate detection and monitoring through observing antibiotic resistance, fluorescence, and bioluminescence...
July 10, 2017: Bioengineered
https://www.readbyqxmd.com/read/28662363/polyvinylsulfonic-acid-a-low-cost-rnase-inhibitor-for-enhanced-rna-preservation-and-cell-free-protein-translation
#4
Conner C Earl, Mark T Smith, Richard A Lease, Bradley C Bundy
The effectiveness and economics of polyvinyl sulfonic acid (PVSA) as a ribonuclease inhibitor for in vitro systems is reported. PVSA was shown to inhibit RNA cleavage in the presence of RNase A as well as in the presence of Escherichia coli lysate, suggesting that PVSA can act as a broader ribonuclease inhibitor. In addition, PVSA was shown to improve the integrity of mRNA transcripts by up to 5-fold in vitro as measured by their translational viability. Improved preservation of mRNA transcripts in the presence of PVSA under common RNA storage conditions is also reported...
June 29, 2017: Bioengineered
https://www.readbyqxmd.com/read/28644714/genome-mining-in-silico-validation-and-phase-selection-of-a-novel-aldo-keto-reductase-from-candida-glabratea-for-biotransformation
#5
Souvik Basak, Nanda Gopal Sahoo, Angayar K Pavanasam
Previously, we published cloning, over-expression, characterization and subsequent exploitation of a carbonyl reductase (cr) gene, belonging to general family aldo-keto reductase from Candida glabrata CBS138 to convert keto ester (COBE) to a chiral alcohol (ethyl-4-chloro-3-hydroxybutanoate or CHBE). Exploiting global transcription factor CRP, rDNA and transporter engineering, we have improved batch production of CHBE by trinomial bioengineering. Herein, we present the exploration of cr gene in Candida glabrata CBS138 through genome miningapproach, in silico validation of its activity and selection of its biocatalytic phase...
June 23, 2017: Bioengineered
https://www.readbyqxmd.com/read/28632991/uncovering-the-repertoire-of-fungal-secondary-metabolites-from-fleming-s-laboratory-to-the-international-space-station
#6
Tomasz Boruta
Fungi produce a variety of secondary metabolites (SMs), low-molecular weight compounds associated with many potentially useful biologic activities. The examples of biotechnologically relevant fungal metabolites include penicillin, a β-lactam antibiotic, and lovastatin, a cholesterol-lowering drug. The discovery of pharmaceutical lead compounds within the microbial metabolic pools relies on the selection and biochemical characterization of promising strains. Not all SMs are produced under standard cultivation conditions, hence the uncovering of chemical potential of investigated strains often requires the use of induction strategies to awake the associated biosynthetic genes...
June 20, 2017: Bioengineered
https://www.readbyqxmd.com/read/28594266/simple-fluorescence-based-detection-of-protein-kinase-a-activity-using-a-molecular-beacon-probe
#7
Changbei Ma, Xiaoyuan Lv, Kemin Wang, Shunxin Jin, Haisheng Liu, Kefeng Wu, Weimin Zeng
Protein kinase A was detected by quantifying the amount of ATP utilized after a protein kinase reaction. The ATP assay was performed using the T4 DNA ligase and a molecular beacon (MB). In the presence of ATP, DNA ligase catalyzed the ligation of short DNA. The ligation product then hybridized to MB, resulting in a fluorescence enhancement of the MB. This assay was capable of determining protein kinase A in the range of 12.5∼150 nM, with a detection limit of 1.25 nM. Furthermore, this assay could also be used to investigate the effect of genistein on protein kinase A...
June 8, 2017: Bioengineered
https://www.readbyqxmd.com/read/28574746/microbial-production-of-mevalonate-by-recombinant-escherichia-coli-using-acetic-acid-as-a-carbon-source
#8
Xin Xu, Meng Xie, Qian Zhao, Mo Xian, Huizhou Liu
We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06 g L(-1) mevalonate with a productivity of 0.03 g L(-1) h(-1) in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7...
June 2, 2017: Bioengineered
https://www.readbyqxmd.com/read/28509615/mining-of-a-phospholipase-d-and-its-application-in-enzymatic-preparation-of-phosphatidylserine
#9
Wen-Bin Zhou, Jin-Song Gong, Hai-Juan Hou, Heng Li, Zhen-Ming Lu, Hong-Yu Xu, Zheng-Hong Xu, Jin-Song Shi
Phosphatidylserine (PS) is useful as the additive in industries for memory improvement, mood enhancement and drug delivery. Conventionally, PS was extracted from soybeans, vegetable oils, egg yolk, and biomass; however, their low availability and high extraction cost were limiting factors. Phospholipase D (PLD) is a promising tool for enzymatic synthesis of PS due to its transphosphatidylation activity. In this contribution, a new and uncharacterized PLD was first obtained from GenBank database via genome mining strategy...
May 16, 2017: Bioengineered
https://www.readbyqxmd.com/read/28494182/fabg-can-function-as-phab-for-poly-3-hydroxybutyrate-biosynthesis-in-photosynthetic-cyanobacteria-synechocystis-sp-pcc-6803
#10
Haowei Zhang, Yinghui Liu, Changhong Yao, Xupeng Cao, Jing Tian, Song Xue
The production of poly-3-hydroxybutyrate (PHB) by photosynthetic cyanobacteria is a potentially sustainable production method for the biodegradable plastics industry. β-Ketoacyl-ACP reductase (FabG), from the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 (SpFabG), is the first NADPH-dependent reductase in the fatty acid biosynthesis pathway. Its structure is similar to that of acetoacetyl-CoA reductase (SpPhaB), which is critical for PHB synthesis and can replace SpPhaB for acetoacetyl-CoA reduction in vitro...
May 11, 2017: Bioengineered
https://www.readbyqxmd.com/read/28471319/enhanced-microalgal-lipid-production-with-media-engineering-of-potassium-nitrate-as-a-nitrogen-source
#11
Rakesh Singh Gour, Madhusudan Bairagi, Vijay Kumar Garlapati, Anil Kant
Algal biofuels are far from a commercial reality due to the technical challenges associated with their growth and lipid extraction procedures. In this study, we investigated the effect of 4 different media and 5 different nitrogen sources at 5 levels on the growth, biomass and lipid productivity of Scenedesmus sp and Chlorella sp The hypothesis was that a nitrogen source can be identified that provides enough stress to accumulate lipids without compromising significantly on biomass and lipid productivity. A maximum specific growth rate and doubling per day have been observed with algal species using modified BG-11 medium...
May 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/28471311/a-synthetic-hybrid-promoter-for-d-xylonate-production-at-low-ph-in-the-tolerant-yeast-candida-glycerinogenes
#12
Hao Ji, Xinyao Lu, Hong Zong, Bin Zhuge
The tolerant yeast Candida glycerinogenes, with high D-xylonate and low-pH tolerances, was used as the host for D-xylonate production at low pH in this study. A low-pH inducible promoter, pGUKd, was engineered using the core promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (pGAP) combined with the upstream activating sequence of the promoter of the guanylate kinase gene (pGUK1) that had substituted pH-responsive TF binding sites. The recombinant cells that expressed GFP from the hybrid promoter pGUKd displayed dramatically increased fluorescence intensity at pH 2...
May 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/28471292/statistical-optimization-for-the-production-of-recombinant-cold-adapted-superoxide-dismutase-in-e-coli-using-response-surface-methodology
#13
Yatong Wang, Quanfu Wang, Yifan Wang, Han Han, Yanhua Hou, Yonglei Shi
Cold-adapted superoxide dismutase (SOD) with higher catalytic activity at lower temperature has great amount of applications in many aspects as an industrial enzyme. The application of recombinant enzyme in gene engineering and microbial fermentation technology is an effective way to obtain high-yield product. In this study, to obtain the recombinant SOD in E. coli (rPsSOD) with the highest activity, the Box-Behnken design was first applied to optimize the important parameters (lactose, tryptone and Tween-80) affecting the activity of rPsSOD...
May 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/28463573/bioengineering-toward-direct-production-of-immobilized-enzymes-a-paradigm-shift-in-biocatalyst-design
#14
Fabian B H Rehm, Shuxiong Chen, Bernd H A Rehm
The need for cost-effectively produced and improved biocatalysts for industrial, pharmaceutical and environmental processes is steadily increasing. While enzyme properties themselves can be improved via protein engineering, immobilization by attachment to carrier materials remains a critical step for stabilization and process implementation. A new emerging immobilization approach, the in situ immobilization, enables simultaneous production of highly active enzymes and carrier materials using bioengineering/synthetic biology of microbial cells...
May 2, 2017: Bioengineered
https://www.readbyqxmd.com/read/28453429/anti-microbial-coating-innovations-to-prevent-infectious-diseases-amici-cost-action-ca15114
#15
Colum P Dunne, Minna M Keinänen-Toivola, Anne Kahru, Birgit Teunissen, Hulya Olmez, Isabel Gouveia, Luis Melo, Kazimierz Murzyn, Martina Modic, Merja Ahonen, Pete Askew, Theofilos Papadopoulos, Christian Adlhart, Francy R L Crijns
Worldwide, millions of patients are affected annually by healthcare-associated infection (HCAI), impacting up to 80,000 patients in European Hospitals on any given day. This represents not only public health risk, but also an economic burden. Complementing routine hand hygiene practices, cleaning and disinfection, antimicrobial coatings hold promise based, in essence, on the application of materials and chemicals with persistent bactericidal or -static properties onto surfaces or in textiles used in healthcare environments...
April 28, 2017: Bioengineered
https://www.readbyqxmd.com/read/28453385/performance-improvement-of-microbial-fuel-cell-mfc-using-suitable-electrode-and-bioengineered-organisms-a-review
#16
Payel Choudhury, Uma Shankar Prasad Uday, Tarun Kanti Bandyopadhyay, Rup Narayan Ray, Biswanath Bhunia
There is an urgent need to find an environment friendly and sustainable technology for alternative energy due to rapid depletion of fossil fuel and industrialization. Microbial Fuel Cells (MFCs) have operational and functional advantages over the current technologies for energy generation from organic matter as it directly converts electricity from substrate at ambient temperature. However, MFCs are still unsuitable for high energy demands due to practical limitations. The overall performance of an MFC depends on microorganism, appropriate electrode materials, suitable MFC designs, and optimizing process parameters which would accelerate commercialization of this technology in near future...
April 28, 2017: Bioengineered
https://www.readbyqxmd.com/read/28453384/isolation-and-characterization-of-ulva-prolifera-actin1-gene-and-function-verification-of-the-5-flanking-region-as-a-strong-promoter
#17
Chunhui Wu, Peng Jiang, Yang Guo, Jianguo Liu, Jin Zhao, Huihui Fu
Ulva prolifera is a green macroalgae with an extremely high growth rate that can accumulate biomass with considerable protein content. To set up an available seaweed expression system, a prior step is to isolate endogenous and strong constitutive promoters. For this reason, the full-length genomic actin1 gene from U. prolifera (Upactin1) was cloned and its 5' flanking sequence was obtained by genome walking. The Upactin1 open reading frame consisted of 1134 nucleotides encoding 377 amino acid residues. Besides 4 exons and 3 introns in the coding region, an extra leader intron was identified in the 5' untranslated region...
April 28, 2017: Bioengineered
https://www.readbyqxmd.com/read/28448741/chromophore-attachment-to-fusion-protein-of-streptavidin-and-recombinant-allophycocyanin-%C3%AE-subunit
#18
Jing Wu, Huaxin Chen, Peng Jiang
The fusion protein (SLA) of streptavidin and allophycocyanin α subunit (holo-ApcA) was biosynthesized in Escherichia coli by a dual plasmid system. The recombinant SLA, purified by affinity chromatography, showed spectral properties similar to natural allophycocyanin α subunit (ApcA). Spectral and Zinc staining analysis indicated that the recombinant SLA covalently bound phycocyanobilin (PCB). To improve chromophorylation rate of recombinant SLA, an in vitro chromophore attachment reaction system was established, which contained partially chromophylated SLA, PCB and lyase CpcS...
April 27, 2017: Bioengineered
https://www.readbyqxmd.com/read/28581909/development-of-crispr-cas9-mediated-virus-resistance-in-agriculturally-important-crops
#19
Surender Khatodia, Kirti Bhatotia, Narendra Tuteja
Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) system of targeted genome editing has already revolutionized the plant science research. This is a RNA guided programmable endonuclease based system composed of 2 components, the Cas9 nuclease and an engineered guide RNA targeting any DNA sequence of the form N20-NGG for novel genome editing applications. The CRISPR/Cas9 technology of targeted genome editing has been recently applied for imparting virus resistance in plants...
May 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/28453368/establishment-of-expanded-and-streamlined-pipeline-of-pitch-knock-in-a-web-based-design-tool-for-mmej-mediated-gene-knock-in-pitch-designer-and-the-variations-of-pitch-pitch-tg-and-pitch-kiko
#20
Kazuki Nakamae, Yuki Nishimura, Mitsumasa Takenaga, Shota Nakade, Naoaki Sakamoto, Hiroshi Ide, Tetsushi Sakuma, Takashi Yamamoto
The emerging genome editing technology has enabled the creation of gene knock-in cells easily, efficiently, and rapidly, which has dramatically accelerated research in the field of mammalian functional genomics, including in humans. We recently developed a microhomology-mediated end-joining-based gene knock-in method, termed the PITCh system, and presented various examples of its application. Since the PITCh system only requires very short microhomologies (up to 40 bp) and single-guide RNA target sites on the donor vector, the targeting construct can be rapidly prepared compared with the conventional targeting vector for homologous recombination-based knock-in...
May 4, 2017: Bioengineered
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