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Bioengineered

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https://www.readbyqxmd.com/read/28140759/expression-of-thermostable-%C3%AE-xylosidase-in-escherichia-coli-for-use-in-saccharification-of-plant-biomass
#1
Muhammad N Aftab, Asma Zafar, Ali R Awan
The present work is aimed to evaluate the saccharification potential of a thermostable β-xylosidase cloned from Bacillus licheniformis into Escherichia coli for production of bioethanol from plant biomass. Recombinant β-xylosidase enzyme possesses the ability of bioconversion of plant biomass like wheat straw, rice straw and sugarcane bagass. By using this approach, plant biomass that mainly constitute cellulose can be converted to reducing sugars that could then be easily converted to bioethanol by simple fermentation process...
January 31, 2017: Bioengineered
https://www.readbyqxmd.com/read/28140758/diversity-of-culturable-bacteria-in-deep-sea-water-from-the-south-atlantic-ocean
#2
Wang Kai, Yan Peisheng, Ma Rui, Jia Wenwen, Shao Zongze
The variability of culturable bacterial diversity and distribution was studied by phylogenetic analysis of 16S rRNA sequences. Seventeen water samples were examined and were collected, from different depths in the range of 5 m to 2700 m at three sampling sites (CTD06, CTD10 and CTD11) in the South Atlantic Ocean. Phylogenetic analysis of 16S rRNA gene sequences revealed a significant diversity of culturable bacteria. A total of 247 strains clustered into eight classes: γ-Proteobacteria, α-Proteobacteria, Actinobacteria, Actinomycetales, Bacilli, Flavobacteria, Opitutae and Sphingobacteria...
January 31, 2017: Bioengineered
https://www.readbyqxmd.com/read/28140757/structure-and-bioactivities-of-a-polysaccharide-isolated-from-ganoderma-lucidum-in-submerged-fermentation
#3
Aisikaer Ai-Lati, Shuangping Liu, Zhongwei Ji, Hao Zhang, Jian Mao
In this study, a Ganoderma lucidum polysaccharide GLP-1-1 was isolated from a culture broth with Mw of 22014 Da. Monosaccharide contained glucose, mannose, and galactose with mole percentages of 92.33%, 7.55%, and 0.22%, respectively. Moreover, FTIR and methylation analysis were conducted to characterize the structural properties of GLP-1-1. The results of antioxidant activity analysis showed that GLP-1-1 had a great DPPH and ABTS radical scavenging activity. Meanwhile, GLP-1-1 also exhibited anti-tumor activity to A431 and MDA-MB-231 cells, and inhibitory rates were dose-dependent...
January 31, 2017: Bioengineered
https://www.readbyqxmd.com/read/28140749/aberrant-methylated-ednrb-can-act-as-a-potential-diagnostic-biomarker-in-sporadic-colorectal-cancer-while-kiss1-is-controversial
#4
Reza Mousavi Ardehaie, Shahriar Hashemzadeh, Shahin Behrouz Sharif, Morteza Ghojazadeh, Ladan Teimoori-Toolabi, Ebrahim Sakhinia
Cancers are among the most serious threats of human health worldwide. Survival and mortality rates of colorectal cancer (CRC) strongly depend on the early diagnosis. The aberrant methylation pattern of genes as a diagnostic biomarker can serve as a practical option for timely detection and contribute subsequently to the enhancement of survival rate in CRC patients, since methylation changes are not only frequent but also can occur in initial tumorogenesis stages. It has been indicated that EDNRB and KISS1 genes are hypermethylated through progression and development of CRC...
January 31, 2017: Bioengineered
https://www.readbyqxmd.com/read/28140746/cas9-cpf1-and-c2c1-2-3-what-s-next
#5
Shota Nakade, Takashi Yamamoto, Tetsushi Sakuma
Since the rapid emergence of clustered regulatory interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system, developed as a genome engineering tool in 2012-2013, most researchers in the life science field have had a fixated interest in this fascinating technology. CRISPR-Cas9 is an RNA-guided DNA endonuclease system, which consists of Cas9 nuclease defining a few targeting base via protospacer adjacent motif complexed with easily customizable single guide RNA targeting around 20-bp genomic sequence...
January 31, 2017: Bioengineered
https://www.readbyqxmd.com/read/28121482/functional-display-of-ice-nucleation-protein-inaz-on-the-surface-of-bacterial-ghosts
#6
Johannes Kassmannhuber, Mascha Rauscher, Lea Schöner, Angela Witte, Werner Lubitz
In a concept study the ability to induce heterogeneous ice formation by Bacterial Ghosts (BGs) from Escherichia coli carrying ice nucleation protein InaZ from Pseudomonas syringae in their outer membrane was investigated by a droplet-freezing assay of ultra-pure water. As determined by the median freezing temperature and cumulative ice nucleation spectra it could be demonstrated that both the living recombinant E. coli and their corresponding BGs functionally display InaZ on their surface. Under the production conditions chosen both samples belong to type II ice-nucleation particles inducing ice formation at a temperature range of between -5...
January 25, 2017: Bioengineered
https://www.readbyqxmd.com/read/28102738/optimization-and-partial-characterization-of-intracellular-anticandidal-protein-from-aspergillus-giganteus-mtcc-8408-using-taguchi-doe
#7
Debashis Dutta, Mira Debnath Das
A new intracellular antifungal protein (afp) production with average molecular weight 24.3 kDa and yield of 0.65 ± 0.1 mg/gram dry cell weight (gdcw) of mycelia in submerged fermentation of Aspergillus giganteus MTCC 8408 was optimized. Taguchi's DOE (design of experiment) L27 orthogonal array (OA) was constructed using Qualitek-4 software with 8 most influensive factors namely, culture pH, temperature, slant age, inoculum volume, agitation and KH2PO4. Scanning electron microscopy (SEM) was used to correlate the effect of selected factors on fungal cell morphology and afp production...
January 19, 2017: Bioengineered
https://www.readbyqxmd.com/read/28095107/bioremediation-of-petroleum-wastewater-by-hyper-phenol-tolerant-bacillus-cereus-preliminary-studies-with-laboratory-scale-batch-process
#8
Aditi Banerjee, Aloke K Ghoshal
Petroleum wastewater samples from oil refinery and oil exploration site were treated by hyper phenol-tolerant Bacillus cereus (AKG1 and AKG2) in laboratory-scale batch process to assess their bioremediation efficacy. Quality of the treated wastewater samples were analyzed in terms of removal of chemical oxygen demand (COD), total organic carbon (TOC) and ammonium nitrogen content, and improvement of biological oxygen demand (BOD). Adaptation of these bacteria to the toxic environment through structural changes in their cell membranes was also highlighted...
January 17, 2017: Bioengineered
https://www.readbyqxmd.com/read/28075663/comparative-study-of-her-2-p53-ki-67-expression-and-clinicopathological-characteristics-of-breast-cancer-in-a-cohort-of-northern-china-female-patients
#9
Li Ding, Zijin Zhang, Yan Xu, Yongqiang Zhang
OBJECTIVE: To study the relationship among Her-2, Ki-67, p53 expression and the clinicopathologic characteristics of breast cancer in the patients of northern China. METHODS: Expression of Her-2, Ki-67, p53 and clinical characteristics of 260 breast cancer patients were retrospectively studied. RESULTS: Her-2 overexpression led to higher incidence rates of infiltrating ductal carcinoma and axillary lymph node metastasis, bigger diameters of the primary tumors, later pTNM staging, and a lower incidence rate of ductal carcinoma in situ (p < 0...
January 11, 2017: Bioengineered
https://www.readbyqxmd.com/read/28071976/precise-flexible-and-affordable-gene-stacking-for-crop-improvement
#10
Weiqiang Chen, David W Ow
Addendum to: Hou L, Yau YY, Wei J, Han Z, Dong Z, Ow DW. An open-source system for in planta gene stacking by Bxb1 and Cre recombinases. Mol Plant, 2014; 7(12):1756-1765. doi:10.1093/mp/ssu107; Li R, Han Z, Hou L, Kaur G, Yin Q, Ow DW. Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase. In: Murata M, editor. Chromosome and Genomic Engineering in Plants: Methods and Protocols, Methods in Molecular Biology. 2016; p.15-30; and Chen W, Ow DW. Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase-Mediated Gene Stacking...
January 10, 2017: Bioengineered
https://www.readbyqxmd.com/read/28051906/clinicopathological-significance-of-survivin-expression-in-patients-with-cervical-cancer-a-systematic-meta-analysis
#11
Yibing Fan, Juan Chen
BACKGROUND: Survivin has been shown to play an important role in cancer pathogenesis. However, its role in cervical cancer development is still controversial. This study was performed to evaluate the clinical significance of survivin expression in cervical cancer. METHODS: Search of some online electronic databases was conducted to identify available studies. The pooled odds ratios (ORs) with its 95% confidence intervals (CIs) were calculated and analyzed. RESULTS: Finally, 18 eligible studies with 791 cervical cancer patients, 1,013 cervical intraepithelial neoplasia (CIN) lesions, 199 normal cervical tissues, and 95 samples with chronic cervicitis were identified in this analysis...
January 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/28045587/conjugation-of-%C3%AF-bt1-derived-integrative-plasmid-pdzl802-in-amycolatopsis-mediterranei-u32
#12
Chen Li, Li Zhou, Ying Wang, Guoping Zhao, Xiaoming Ding
The genus Amycolatopsis is well known for its ability to produce antibiotics, and an increasing number of valuable biotechnological applications, such as bioremediation, biodegradation, bioconversion, and potentially biofuel, that use this genus have been developed. Amycolatopsis mediterranei is an industrial-scale producer of the important antibiotic rifamycin, which plays a vital role in antimycobacterial therapy. Genetic studies of Amycolatopsis species have progressed slowly due to the lack of efficient transformation methods and stable plasmid vectors...
January 3, 2017: Bioengineered
https://www.readbyqxmd.com/read/27937123/metabolic-engineering-of-saccharomyces-cerevisiae-for-improvement-in-stresses-tolerance
#13
Nileema R Divate, Gen-Hung Chen, Rupesh D Divate, Bor-Rung Ou, Yun-Chin Chung
Lignocellulosic biomass is an attractive low-cost feedstock for bioethanol production. During bioethanol production, Saccharomyces cerevisiae, the common used starter, faces several environmental stresses such as aldehydes, glucose, ethanol, high temperature, acid, alkaline and osmotic pressure. The aim of this study was to construct a genetic recombinant S. cerevisiae starter with high tolerance against various environmental stresses. Trehalose-6-phosphate synthase gene (tps1) and aldehyde reductase gene (ari1) were co-overexpressed in nth1 (coded for neutral trehalase gene, trehalose degrading enzyme) deleted S...
December 12, 2016: Bioengineered
https://www.readbyqxmd.com/read/27893301/optimizing-the-expression-of-a-heterologous-chitinase-a-study-of-different-promoters
#14
Abigail F da Silva, Belén García-Fraga, Jacobo López-Seijas, Carmen Sieiro
Many relevant applications have been demonstrated for chitinolytic enzymes. However, their successful exploitation depends upon the availability of strains and expression conditions that allow the production of active forms and large quantities of these enzymes. Escherichia coli has been commonly used to express and overproduce different proteins, among them chitinases. Improving the functional gene expression of chitinases is key to exploiting their potential. In a recent study, we described the effect of various parameters on the functional expression of 2 chitinases from different families, demonstrating that the effect of each of these parameters on the activity of both chitinases was specific to each enzyme...
November 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27791460/effect-of-testosterone-on-the-proliferation-and-collagen-synthesis-of-cardiac-fibroblasts-induced-by-angiotensin-ii-in-neonatal-rat
#15
Xiaocun Yang, Ying Wang, Shuxun Yan, Lina Sun, Guojie Yang, Yuan Li, Chaonan Yu
The objective is to explore the effect of testosterone on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts (CF) induced by Angiotensin II (Ang II) and the underlying mechanisms. Derived from neonatal rats, the CFs were divided into 4 groups: the control group, Ang II group, testosterone group, and testosterone + Ang II group in vitro. Cell cycle distribution, collagen counts, and phosphorylated extracellular signal-regulated kinase (ERK1/2) (p - ERK1/2) expression were assessed by flow cytometry, VG staining, and immunocytochemistry, respectively...
January 2, 2017: Bioengineered
https://www.readbyqxmd.com/read/27775502/a-study-on-detection-of-glucose-concentration-using-changes-in-color-coordinates
#16
Ji-Sun Kim, Han-Byeol Oh, A-Hee Kim, Jun-Sik Kim, Eun-Suk Lee, Jin-Young Baek, Ki Sung Lee, Soon-Cheol Chung, Jae-Hoon Jun
Glucose concentration is closely related to the metabolic activity of cells and it is the most important substance as the energy source of a living body which plays an important role in the human body. This paper proposes an optical method that can measure the concentration of glucose. The change in glucose concentration was observed by using CIE diagram, and wavelength and purity values were detected. Also, even small changes in glucose concentration can be evaluated through mathematical modeling. This system is simple, economical, and capable of quantifying optical signals with numerical values for glucose sensing...
January 2, 2017: Bioengineered
https://www.readbyqxmd.com/read/27764542/optic-cup-segmentation-from-fundus-images-for-glaucoma-diagnosis
#17
Man Hu, Chenghao Zhu, Xiaoxing Li, Yongli Xu
Glaucoma is a serious disease that can cause complete, permanent blindness, and its early diagnosis is very difficult. In recent years, computer-aided screening and diagnosis of glaucoma has made considerable progress. The optic cup segmentation from fundus images is an extremely important part for the computer-aided screening and diagnosis of glaucoma. This paper presented an automatic optic cup segmentation method that used both color difference information and vessel bends information from fundus images to determine the optic cup boundary...
January 2, 2017: Bioengineered
https://www.readbyqxmd.com/read/27759485/synthesis-and-biological-activity-of-the-novel-indanedione-anticoagulant-rodenticides-containing-fluorine
#18
Feng Chen, Liping Liu, Zengguo Bai, Tianhua Zhang, Keke Zhao
Here, 3 fluorinated intermediates of drug were synthesized: (M1), (M2), (M3). Three new anticoagulant rodenticides were designed which were based on 4-hydroxycoumarin or 1,3-indandione, added acute toxicity groups containing fluorine. The structures of synthesized compounds were analyzed and proved by FT-IR spectroscopy and (1)H nuclear magnetic resonance ((1)H-NMR). The compounds were also evaluated for their anticoagulant and acute biologic activity. In addition, both the acute orally toxicity and the feeding indexes of R1 and R2 were tested...
January 2, 2017: Bioengineered
https://www.readbyqxmd.com/read/27753530/friction-properties-of-biological-functional-materials-pvdf-membranes
#19
Long Chen, Changan Di, Xuguang Chen, Zhengzhi Li, Jia Luo
Touch is produced by sensations that include approaching, sliding, pressing, and temperature. This concept has become a target of research in biotechnology, especially in the field of bionic biology. This study measured sliding and pressing with traditional tactile sensors in order to improve a machine operator's judgment of surface roughness. Based on the theory of acoustic emission, this study combined polyvinylidene fluoride (PVDF) with a sonic transducer to produce tactile sensors that can detect surface roughness...
January 2, 2017: Bioengineered
https://www.readbyqxmd.com/read/27696938/a-novel-modelling-and-simulation-method-of-hip-joint-surface-contact-stress
#20
Monan Wang, Lei Wang, Pengcheng Li, Yili Fu
Understanding the hip joint surface contact stress distribution characteristics is helpful to determine hip joint biomechanical features and abnormal pathological behavior. Firstly, a 3-dimensional static hip joint biomechanical model is built using analytical method of model in order to study biomechanical properties including bearing area, stress distribution and the peak value of the contact stress of the femoral head, which reveals the relationship between the biomechanical properties and its geometric parameters...
January 2, 2017: Bioengineered
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