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Bioengineered

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https://www.readbyqxmd.com/read/28820295/corrigendum
#1
(no author information available yet)
No abstract text is available yet for this article.
August 18, 2017: Bioengineered
https://www.readbyqxmd.com/read/28708015/relationship-between-ppar%C3%AE-mrna-expression-and-mitochondrial-respiratory-function-and-ultrastructure-of-the-skeletal-muscle-of-patients-with-copd
#2
Jian-Qing Zhang, Xiang-Yu Long, Yu Xie, Zhi-Huan Zhao, Li-Zhou Fang, Ling Liu, Wei-Ping Fu, Jing-Kui Shu, Jiang-Hai Wu, Lu-Ming Dai
Peripheral muscle dysfunction is an important complication in patients with chronic obstructive pulmonary disease (COPD). The objective of this study was to explore the relationship between the levels of peroxisome proliferator-activated receptor α (PPARα) mRNA expression and the respiratory function and ultrastructure of mitochondria in the vastus lateralis of patients with COPD. Vastus lateralis biopsies were performed on 14 patients with COPD and 6 control subjects with normal lung function. PPARα mRNA levels in the muscle tissue were detected by real-time PCR...
July 14, 2017: Bioengineered
https://www.readbyqxmd.com/read/28696140/regulatory-roles-of-24-epibrassinolide-in-tolerance-of-acacia-gerrardii-benth-to-salt-stress
#3
Elsayed Fathi Abd Allah, A A Alqarawi, Abeer Hashem, Stephan Wirth, Dilfuza Egamberdieva
This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaCl-induced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1.0 mg/l; each plant received 2.5 ml) mitigated the negative effect considerably. NaCl reduced chlorophyll content but this was significantly ameliorated by the application of EBL. EBL reduced significantly NaCl-induced oxidative stress hence protect membranes and also improved the relative water content significantly by 6...
July 11, 2017: Bioengineered
https://www.readbyqxmd.com/read/28692329/surrogate-strains-of-human-pathogens-for-field-release
#4
Sangjin Park, Chang-Hwan Kim, Seong Tae Jeong, Sang Yup Lee
Surrogate microorganisms, in short surrogates, are an essential part of pathogen research. Compared to surrogates used in controlled laboratory environments, surrogates for field release are restricted by concerns about human and environmental safety. For field research of food-borne pathogens, strains of an attenuated pathogen or strains of genetically close non-pathogenic species have been used as surrogates. Genetic modification is usually performed to attenuate virulence, through for examples deletion of genes of virulence and transcriptional regulators and removal of virulence plasmids, and to facilitate detection and monitoring through observing antibiotic resistance, fluorescence, and bioluminescence...
July 10, 2017: Bioengineered
https://www.readbyqxmd.com/read/28662363/polyvinylsulfonic-acid-a-low-cost-rnase-inhibitor-for-enhanced-rna-preservation-and-cell-free-protein-translation
#5
Conner C Earl, Mark T Smith, Richard A Lease, Bradley C Bundy
The effectiveness and economics of polyvinyl sulfonic acid (PVSA) as a ribonuclease inhibitor for in vitro systems is reported. PVSA was shown to inhibit RNA cleavage in the presence of RNase A as well as in the presence of Escherichia coli lysate, suggesting that PVSA can act as a broader ribonuclease inhibitor. In addition, PVSA was shown to improve the integrity of mRNA transcripts by up to 5-fold in vitro as measured by their translational viability. Improved preservation of mRNA transcripts in the presence of PVSA under common RNA storage conditions is also reported...
June 29, 2017: Bioengineered
https://www.readbyqxmd.com/read/28644714/genome-mining-in-silico-validation-and-phase-selection-of-a-novel-aldo-keto-reductase-from-candida-glabratea-for-biotransformation
#6
Souvik Basak, Nanda Gopal Sahoo, Angayar K Pavanasam
Previously, we published cloning, over-expression, characterization and subsequent exploitation of a carbonyl reductase (cr) gene, belonging to general family aldo-keto reductase from Candida glabrata CBS138 to convert keto ester (COBE) to a chiral alcohol (ethyl-4-chloro-3-hydroxybutanoate or CHBE). Exploiting global transcription factor CRP, rDNA and transporter engineering, we have improved batch production of CHBE by trinomial bioengineering. Herein, we present the exploration of cr gene in Candida glabrata CBS138 through genome miningapproach, in silico validation of its activity and selection of its biocatalytic phase...
June 23, 2017: Bioengineered
https://www.readbyqxmd.com/read/28632991/uncovering-the-repertoire-of-fungal-secondary-metabolites-from-fleming-s-laboratory-to-the-international-space-station
#7
Tomasz Boruta
Fungi produce a variety of secondary metabolites (SMs), low-molecular weight compounds associated with many potentially useful biologic activities. The examples of biotechnologically relevant fungal metabolites include penicillin, a β-lactam antibiotic, and lovastatin, a cholesterol-lowering drug. The discovery of pharmaceutical lead compounds within the microbial metabolic pools relies on the selection and biochemical characterization of promising strains. Not all SMs are produced under standard cultivation conditions, hence the uncovering of chemical potential of investigated strains often requires the use of induction strategies to awake the associated biosynthetic genes...
June 20, 2017: Bioengineered
https://www.readbyqxmd.com/read/28594266/simple-fluorescence-based-detection-of-protein-kinase-a-activity-using-a-molecular-beacon-probe
#8
Changbei Ma, Xiaoyuan Lv, Kemin Wang, Shunxin Jin, Haisheng Liu, Kefeng Wu, Weimin Zeng
Protein kinase A was detected by quantifying the amount of ATP utilized after a protein kinase reaction. The ATP assay was performed using the T4 DNA ligase and a molecular beacon (MB). In the presence of ATP, DNA ligase catalyzed the ligation of short DNA. The ligation product then hybridized to MB, resulting in a fluorescence enhancement of the MB. This assay was capable of determining protein kinase A in the range of 12.5∼150 nM, with a detection limit of 1.25 nM. Furthermore, this assay could also be used to investigate the effect of genistein on protein kinase A...
June 8, 2017: Bioengineered
https://www.readbyqxmd.com/read/28574746/microbial-production-of-mevalonate-by-recombinant-escherichia-coli-using-acetic-acid-as-a-carbon-source
#9
Xin Xu, Meng Xie, Qian Zhao, Mo Xian, Huizhou Liu
We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06 g L(-1) mevalonate with a productivity of 0.03 g L(-1) h(-1) in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7...
June 2, 2017: Bioengineered
https://www.readbyqxmd.com/read/28277159/corrigendum
#10
(no author information available yet)
No abstract text is available yet for this article.
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/28075663/comparative-study-of-her-2-p53-ki-67-expression-and-clinicopathological-characteristics-of-breast-cancer-in-a-cohort-of-northern-china-female-patients
#11
Li Ding, Zijin Zhang, Yan Xu, Yongqiang Zhang
The objective was to study the relationship among Her-2, Ki-67, p53 expression and the clinicopathologic characteristics of breast cancer in the patients of northern China. Expression of Her-2, Ki-67, p53 and clinical characteristics of 260 breast cancer patients were retrospectively studied. Her-2 overexpression led to higher incidence rates of infiltrating ductal carcinoma and axillary lymph node metastasis, bigger diameters of the primary tumors, later pTNM staging, and a lower incidence rate of ductal carcinoma in situ (p < 0...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27893301/optimizing-the-expression-of-a-heterologous-chitinase-a-study-of-different-promoters
#12
Abigail F da Silva, Belén García-Fraga, Jacobo López-Seijas, Carmen Sieiro
Many relevant applications have been demonstrated for chitinolytic enzymes. However, their successful exploitation depends upon the availability of strains and expression conditions that allow the production of active forms and large quantities of these enzymes. Escherichia coli has been commonly used to express and overproduce different proteins, among them chitinases. Improving the functional gene expression of chitinases is key to exploiting their potential. In a recent study, we described the effect of various parameters on the functional expression of 2 chitinases from different families, demonstrating that the effect of each of these parameters on the activity of both chitinases was specific to each enzyme...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27813700/current-challenges-for-the-targeted-delivery-and-molecular-imaging-of-stem-cells-in-animal-models
#13
Arezoo Momeni, Sriram Neelamegham, Natesh Parashurama
In contrast to conventional, molecular medicine that focuses on targeting specific pathways, stem cell therapy aims to perturb many related mechanisms in order to derive therapeutic benefit. This emerging modality is inherently complex due to the variety of cell types that can be used, delivery approaches that need to be optimized in order to target the cellular therapeutic to specific sites in vivo, and non-invasive imaging methods that are needed to monitor cell fate. This review highlights advancements in the field, with focus on recent publications that use preclinical animal models for cardiovascular stem cell therapy...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27791465/production-of-encapsulated-creatinase-using-yeast-spores
#14
Jun Kong, Zijie Li, Huijie Zhang, Xiao-Dong Gao, Hideki Nakanishi
Yeast spores can be used as a carrier to produce enzyme capsules. In the present study, this technique was applied to a diagnostic enzyme named creatinase. We found that a secretory form of Pseudomonas putida creatinase could be entrapped in the spore wall, and such spores were used as creatinase capsules. The activity of the encapsulated creatinase was largely improved by mild spore wall defective mutations, such as DIT1 or OSW2 deletions. The advantages of this method include the following: encapsulated and freeze-dried creatinase is produced without preparing the purified enzyme, and it exhibits resistance to environmental stresses, such as high temperature and SDS treatments...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27791456/ex-vivo-comparative-study-on-three-sinus-lift-tools-for-transcrestal-detaching-maxillary-sinus-mucosa
#15
Yanfeng Li, Pin Hu, Yishi Han, Jiadong Fan, Xinming Dong, Huan Ren, Chunhao Yang, Tingting Shi, Dong Xia
The objective of this study was to comparatively evaluate 3 different sinus lift tools, namely umbrella-shaped sinus lift curette YSL-04, our recently designed probe-improved sinus lift curettes, and our newly invented elevator 014, using our previous developed goat ex vivo models for direct visualizing the effectiveness of detaching sinus mucosa in real time. Goat ex vivo models for direct visualizing the effectiveness of detaching sinus mucosa in real time were generated according to our previously developed protocol...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27791455/in-vitro-and-in-silico-interaction-of-porcine-%C3%AE-amylase-with-vicia-faba-crude-seed-extract-and-evaluation-of-antidiabetic-activity
#16
Dhiraj Kumar Choudhary, Abha Mishra
Diabetes mellitus is a group of metabolic disorder of glucose metabolism. The proper management of blood glucose level is an indicator in the treatment of this complex pathology. The aim of the present study was to evaluate the inhibitory potential of Vicia faba crude seed extracts on the activities of α-amylase. Phytochemical screening, FTIR and HPLC analysis confirmed the presence of a phenolic compound with percentage yield of 9.87% in the acetone extract. Acetone extract of seed had highest inhibitory potential against porcine α-amylase (IC50 value of 2...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27791453/new-biotechnological-applications-for-ashbya-gossypii-challenges-and-perspectives
#17
Tatiana Q Aguiar, Rui Silva, Lucília Domingues
The filamentous fungus Ashbya gossypii has long been considered a paradigm of the White Biotechnology in what concerns riboflavin production. Its industrial relevance led to the development of a significant molecular and in silico modeling toolbox for its manipulation. This, together with the increasing knowledge of its genome and metabolism has helped designing effective metabolic engineering strategies for optimizing riboflavin production, but also for developing new A. gossypii strains for novel biotechnological applications, such as production of recombinant proteins, single cell oils (SCOs), and flavour compounds...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27791452/the-growing-impact-of-lyophilized-cell-free-protein-expression-systems
#18
J Porter Hunt, Seung Ook Yang, Kristen M Wilding, Bradley C Bundy
Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not only overcome cold-storage limitations, but also enable stockpiling for on-demand synthesis and completely sterilize the protein synthesis platform. Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27786573/effect-and-mechanism-of-calpains-on-pediatric-lobar-pneumonia
#19
Genquan Yin, Qiang Zeng, Haijin Zhao, Peiqiong Wu, Shaoxi Cai, Li Deng, Wenhui Jiang
Lobar pneumonia, one of the community-acquired pneumonia (CAP), is a common pediatric low respiratory tract infection. Calpains are Ca(2+)-activated cysteine proteases whose activation mechanism is elusive. The present study was undertaken to detect the role and mechanism of calpains in pediatric lobar pneumonia. The human acute lung infection model (ALIM) was constructed and infected by Streptococcus. Enzyme-linked immunosorbent assay (ELISA) was used to measure interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α...
July 4, 2017: Bioengineered
https://www.readbyqxmd.com/read/27780398/microbial-cooperation-in-the-rhizosphere-improves-liquorice-growth-under-salt-stress
#20
Dilfuza Egamberdieva, Stephan Wirth, Li Li, Elsayed Fathi Abd-Allah, Kristina Lindström
Liquorice (Glycyrrhiza uralensis Fisch.) is one of the most widely used plants in food production, and it can also be used as an herbal medicine or for reclamation of salt-affected soils. Under salt stress, inhibition of plant growth, nutrient acquisition and symbiotic interactions between the medicinal legume liquorice and rhizobia have been observed. We recently evaluated the interactions between rhizobia and root-colonizing Pseudomonas in liquorice grown in potting soil and observed increased plant biomass, nodule numbers and nitrogen content after combined inoculation compared to plants inoculated with Mesorhizobium alone...
July 4, 2017: Bioengineered
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