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https://www.readbyqxmd.com/read/28174694/stat3-and-erk-pathways-are-involved-in-cell-growth-stimulation-of-the-st2-il1rl1-promoter
#1
Kenji Tago, Satoshi Ohta, Megumi Funakoshi-Tago, Chihiro Aoki-Ohmura, Jitsuhiro Matsugi, Shin-Ichi Tominaga, Ken Yanagisawa
The ST2 gene was originally identified as a primary responsive gene induced by stimulation with growth factors and by oncogenic stress. The ST2 gene harbors two distinct promoters - a distal promoter and a proximal promoter. In this study, we identified a novel type of serum-responsive element in the ST2 proximal promoter using reporter gene analysis; this element includes a possible responsive element for STAT family proteins. Indeed, enforced expression of constitutively active STAT3 activated this promoter element and induced the expression of ST2 gene products...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174693/fanconi-anemia-protein-fancd2-is-activated-by-aicar-a-modulator-of-ampk-and-cellular-energy-metabolism
#2
Min Jeong Chun, Hana Choi, Dong Wha Jun, Sunshin Kim, Yong-Nyun Kim, Soo-Youl Kim, Chang-Hun Lee
FANCD2 is a pivotal molecule in the pathogenesis of Fanconi anemia (FA), an autosomal recessive human syndrome with diverse clinical phenotypes, including cancer predisposition, short stature, and hematological abnormalities. In our previous study, we detected the functional association of FANC proteins, whose mutations are responsible for the onset of FA, with AMPK in response to DNA interstrand crosslinking lesions. Because AMPK is well known as a critical sensing molecule for cellular energy levels, we checked whether FANCD2 activation occurs after treatments affecting AMPK and/or cellular energy status...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174692/the-specificity-of-homomeric-clustering-of-cd81-is-mediated-by-its-%C3%AE-loop
#3
Yahya Homsi, Thorsten Lang
Tetraspanins are cell membrane-scaffolding proteins interacting with one another and a repertoire of interaction partners. Through these interactions, they form extended molecular networks as tetraspanin webs or tetraspanin-enriched microdomains. Microscopic data suggest that these networks contain tetraspanin clusters, with poor overlap between clusters formed by different tetraspanins. Here, we investigate the possibility of targeting tetraspanins CD9 or CD151 to clusters formed by the tetraspanin CD81. We find that the δ-loop from the large extracellular domain of CD81 is sufficient for targeting of CD9/CD151 to CD81 clusters...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174691/beta-catenin-participates-in-dialysate-induced-peritoneal-fibrosis-via-enhanced-peritoneal-cell-epithelial-to-mesenchymal-transition
#4
Shuiyu Ji, Hao Deng, Wei Jin, Pengpeng Yan, Rending Wang, Lisha Pang, Jingyi Zhou, Jiaming Zhang, Xiaoying Chen, Xiang Zhao, Jia Shen
Long-term exposure to peritoneal dialysate with high glucose (HG) leads to peritoneal fibrosis and thus decreases dialysis efficiency. In this study, we explored the role of β-catenin in this process. C57BL/6 mice received daily intraperitoneal injection with 10% of the body weight of saline (control), 4.25% glucose peritoneal dialysis fluid (PDF), or PDF combined with 5 mg·kg(-1) of the β-catenin inhibitor ICG-001 (PDF+ICG) for 30 days. Also, mice peritoneal epithelial cells (mPECs) were cultured in 4.25% glucose (HG) or combined with 10 μm ICG-001 (HG+ICG) for 48 h...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174690/the-gene-expression-of-numerous-slc-transporters-is-altered-in-the-immortalized-hypothalamic-cell-line-n25-2-following-amino-acid-starvation
#5
Sofie V Hellsten, Emilia Lekholm, Tauseef Ahmad, Robert Fredriksson
Amino acids are known to play a key role in gene expression regulation, and in mammalian cells, amino acid signaling is mainly mediated via two pathways, the mammalian target of rapamycin complex 1 (mTORC1) pathway and the amino acid responsive (AAR) pathway. It is vital for cells to have a system to sense amino acid levels, in order to control protein and amino acid synthesis and catabolism. Amino acid transporters are crucial in these pathways, due to both their sensing and transport functions. In this large-scale study, an immortalized mouse hypothalamic cell line (N25/2) was used to study the gene expression changes following 1, 2, 3, 5 or 16 h of amino acid starvation...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174689/characterization-of-murine-polyspecific-monoamine-transporters
#6
Yamato Miura, Takeo Yoshikawa, Fumito Naganuma, Tadaho Nakamura, Tomomitsu Iida, Anikó Kárpáti, Takuro Matsuzawa, Asuka Mogi, Ryuichi Harada, Kazuhiko Yanai
The dysregulation of monoamine clearance in the central nervous system occurs in various neuropsychiatric disorders, and the role of polyspecific monoamine transporters in monoamine clearance is increasingly highlighted in recent studies. However, no study to date has properly characterized polyspecific monoamine transporters in the mouse brain. In the present study, we examined the kinetic properties of three mouse polyspecific monoamine transporters [organic cation transporter 2 (Oct2), Oct3, and plasma membrane monoamine transporter (Pmat)] and compared the absolute mRNA expression levels of these transporters in various brain areas...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174688/protection-by-huang-lian-jie-du-decoction-and-its-constituent-herbs-of-lipopolysaccharide-induced-acute-kidney-injury
#7
Pei Li, Shan-Ting Liao, Jun-Song Wang, Qian Zhang, Ding-Qiao Xu, Yan Lv, Ming-Hua Yang, Ling-Yi Kong
Sepsis, characterized by systemic inflammation, often leads to end-organ dysfunction, such as acute kidney injury (AKI). Despite of the severity and frequency of septic AKI in clinic, its pathogenesis is still poorly understood. Combined with histopathology evaluations, mortality assessments, biochemical evaluations, reverse transcription (RT) reaction and quantitative real-time PCR, and western blot, (1)H NMR-based metabolomics approach was applied to investigate effects of Huang-Lian-Jie-Du-Decotion (HLJDD), a traditional Chinese medicine prescription, and its four component herbs on lipopolysaccharide (LPS)-induced septic AKI and the underlying mechanism...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174687/dicer-a-new-regulator-of-pluripotency-exit-and-line-1-elements-in-mouse-embryonic-stem-cells
#8
Maxime Bodak, Daniel Cirera-Salinas, Jian Yu, Richard P Ngondo, Constance Ciaudo
A gene regulation network orchestrates processes ensuring the maintenance of cellular identity and genome integrity. Small RNAs generated by the RNAse III DICER have emerged as central players in this network. Moreover, deletion of Dicer in mice leads to early embryonic lethality. To better understand the underlying mechanisms leading to this phenotype, we generated Dicer-deficient mouse embryonic stem cells (mESCs). Their detailed characterization revealed an impaired differentiation potential, and incapacity to exit from the pluripotency state...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174686/pku-mutation-p-g46s-prevents-the-stereospecific-binding-of-l-phenylalanine-to-the-dimer-of-human-phenylalanine-hydroxylase-regulatory-domain
#9
João Leandro, Jaakko Saraste, Paula Leandro, Torgeir Flatmark
Mammalian phenylalanine hydroxylase (PAH) has a potential allosteric regulatory binding site for l-phenylalanine (l-Phe), in addition to its catalytic site. This arrangement is supported by a crystal structure of a homodimeric truncated form of the regulatory domain of human PAH (hPAH-RD (1-118/19-118)) [Patel D et al. (2016) Sci Rep doi: 10.1038/srep23748]. In this study, a fusion protein of the domain (MBP-(pepXa)-hPAH-RD (1-120)) was overexpressed and recovered in a metastable and soluble state, which allowed the isolation of a dimeric and a monomeric fusion protein...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174685/effects-of-arthrocen-an-avocado-soy-unsaponifiables-agent-on-inflammatory-mediators-and-gene-expression-in-human-chondrocytes
#10
Ramin Goudarzi, Jared F Taylor, Puya G Yazdi, Brian A Pedersen
Osteoarthritis (OA) is a chronic joint disease characterized by pain and stiffness. Recently, there has been great interest in the use of plant-derived compounds and supplements in managing the symptoms of OA. Arthrocen is a plant-based supplement consisting of avocado and soy unsaponifiable extracts in a 1 : 2 ratio. In an effort to unravel the potential mechanisms of its action on the cellular level, we utilized an in vitro assay to study its effects on cultured human chondrocytes. By pairing this assay with protein arrays on inflammatory markers, RNA-Seq with downstream pathway analysis, and lipidomics on eicosanoids, we were able to further define its action at the molecular level...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174684/sucralose-activates-an-erk1-2-ribosomal-protein-s6-signaling-axis
#11
Marcy L Guerra, Michael A Kalwat, Kathleen McGlynn, Melanie H Cobb
The sweetener sucralose can signal through its GPCR receptor to induce insulin secretion from pancreatic β cells, but the downstream signaling pathways involved are not well-understood. Here we measure responses to sucralose, glucagon-like peptide 1, and amino acids in MIN6 β cells. Our data suggest a signaling axis, whereby sucralose induces calcium and cAMP, activation of ERK1/2, and site-specific phosphorylation of ribosomal protein S6. Interestingly, sucralose acted independently of mTORC1 or ribosomal S6 kinase (RSK)...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174683/post-translational-modifications-of-annexin-a2-are-linked-to-its-association-with-perinuclear-nonpolysomal-mrnp-complexes
#12
Ingvild Aukrust, Linn Andersen Rosenberg, Mia Madeleine Ankerud, Vibeke Bertelsen, Hanne Hollås, Jaakko Saraste, Ann Kari Grindheim, Anni Vedeler
Various post-translational modifications (PTMs) regulate the localisation and function of the multifunctional protein Annexin A2 (AnxA2). In addition to its various tasks as a cytoskeletal- and membrane-associated protein, AnxA2 can function as a trans-acting protein binding to cis-acting sequences of specific mRNAs. In the present study, we have examined the role of Ser25 phosphorylation in subcellular localisation of AnxA2 and its interaction with mRNP complexes. Subcellular fractionation and confocal microscopy of rat neuroendocrine PC12 cells showed that Ser25-phosphorylated AnxA2 (pSer25AnxA2) is absent from the nucleus and mainly localised to the perinuclear region, evidently associating with both membranes and cytoskeletal elements...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174682/glucose-deprivation-regulates-the-progranulin-sortilin-axis-in-pc12-cells
#13
Ken-Ichi Kawashima, Yuri Ishiuchi, Miki Konnai, Saori Komatsu, Hitoshi Sato, Hideo Kawaguchi, Nobumitsu Miyanishi, Jérôme Lamartine, Masugi Nishihara, Taku Nedachi
Progranulin (PGRN) is a growth factor implicated in several neurodegenerative diseases, such as frontotemporal lobar degeneration. Despite its important role in the central nervous system (CNS), the mechanisms controlling PGRN expression in the CNS are largely unknown. Recent evidence, however, suggested that several stressors, such as hypoxia, acidosis, or oxidative stress, induce PGRN expression. The present study was mainly aimed at determining whether and, if so, how glucose deprivation affects PGRN expression in PC12 cells...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174681/participation-of-3-o-sulfated-heparan-sulfates-in-the-protection-of-macrophages-by-herpes-simplex-virus-1-glycoprotein-d-and-cyclophilin-b-against-apoptosis
#14
Maxime Delos, Charles Hellec, François Foulquier, Mathieu Carpentier, Fabrice Allain, Agnès Denys
Heparan sulfates (HS) are involved in numerous biological processes, which rely on their ability to interact with a large panel of proteins. Although the reaction of 3-O-sulfation can be catalysed by the largest family of HS sulfotransferases, very few mechanisms have been associated with this modification and to date, only glycoprotein D (gD) of herpes simplex virus-1 (HSV-1 gD) and cyclophilin B (CyPB) have been well-described as ligands for 3-O-sulfated HS. Here, we hypothesized that both ligands could induce the same responses via a mechanism dependent on 3-O-sulfated HS...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28174680/structural-evidence-for-arabidopsis-glutathione-transferase-atgstf2-functioning-as-a-transporter-of-small-organic-ligands
#15
Laziana Ahmad, Elizabeth L Rylott, Neil C Bruce, Robert Edwards, Gideon Grogan
Glutathione transferases (GSTs) are involved in many processes in plant biochemistry, with their best characterised role being the detoxification of xenobiotics through their conjugation with glutathione. GSTs have also been implicated in noncatalytic roles, including the binding and transport of small heterocyclic ligands such as indole hormones, phytoalexins and flavonoids. Although evidence for ligand binding and transport has been obtained using gene deletions and ligand binding studies on purified GSTs, there has been no structural evidence for the binding of relevant ligands in noncatalytic sites...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28097094/corrigendum
#16
(no author information available yet)
[This corrects the article DOI: 10.1002/2211-5463.12131.].
January 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28097093/vascular-endothelial-growth-factor-suppresses-tnfsf15-production-in-endothelial-cells-by-stimulating-mir-31-and-mir-20a-expression-via-activation-of-akt-and-erk-signals
#17
Hui-Ting Deng, Hai-Lin Liu, Bei-Bei Zhai, Kun Zhang, Guo-Ce Xu, Xue-Mei Peng, Qiang-Zhe Zhang, Lu-Yuan Li
Tumor necrosis factor superfamily-15 (TNFSF15; VEGI; TL1A) is a negative modulator of angiogenesis for blood vessel homeostasis and is produced by endothelial cells in a mature vasculature. It is known to be downregulated by vascular endothelial growth factor (VEGF), a major regulator of neovascularization but the mechanism of this interaction is unclear. Here we report that VEGF is able to stimulate the production of two microRNAs, miR-20a and miR-31, which directly target the 3'-UTR of TNFSF15. Additionally, we show that two VEGF-stimulated cell growth signals, Erk and Akt, are responsible for promoting the expression of miR-20a and miR-31...
January 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28097092/comparative-interactomics-for-virus-human-protein-protein-interactions-dna-viruses-versus-rna-viruses
#18
Saliha Durmuş, Kutlu Ö Ülgen
Viruses are obligatory intracellular pathogens and completely depend on their hosts for survival and reproduction. The strategies adopted by viruses to exploit host cell processes and to evade host immune systems during infections may differ largely with the type of the viral genetic material. An improved understanding of these viral infection mechanisms is only possible through a better understanding of the pathogen-host interactions (PHIs) that enable viruses to enter into the host cells and manipulate the cellular mechanisms to their own advantage...
January 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28097091/evaluation-of-novel-protease-inhibitors-against-darunavir-resistant-variants-of-hiv-type-1
#19
Mari Inoue, Daiki Oyama, Koushi Hidaka, Masanori Kameoka
HIV disease became a manageable chronic disease since combination antiretroviral therapy (cART) was introduced as the standard treatment regimen. However, the emergence of drug-resistant viruses is a major problem associated with cART. A phenotypic drug susceptibility test using a lentiviral vector was established and applied to evaluate new protease inhibitors (PIs). Lentiviral vectors representing a wild-type (WT-lentivector) and darunavir (DRV)-resistant HIV type 1 (HIV-1) (DRV (r)-lentivector) were generated...
January 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28097090/a-comparative-study-of-the-degradation-of-yeast-cyclins-cln1-and-cln2
#20
Inma Quilis, J Carlos Igual
The yeast cyclins Cln1 and Cln2 are very similar in both sequence and function, but some differences in their functionality and localization have been recently described. The control of Cln1 and Cln2 cellular levels is crucial for proper cell cycle initiation. In this work, we analyzed the degradation patterns of Cln1 and Cln2 in order to further investigate the possible differences between them. Both cyclins show the same half-life but, while Cln2 degradation depends on ubiquitin ligases SCF(G)(rr1) and SCF(C)(dc4), Cln1 is affected only by SCF(G)(rr1)...
January 2017: FEBS Open Bio
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