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FEBS Open Bio

Ilaria Baglivo, Luciano Pirone, Gaetano Malgieri, Roberto Fattorusso, Roy Martin Roop Ii, Emilia Maria Pedone, Paolo Vincenzo Pedone
The protein MucR from Brucella spp. is involved in the expression regulation of genes necessary for host interaction and infection. MucR is a member of the Ros/MucR family, which comprises prokaryotic zinc-finger proteins and includes Ros from Agrobacterium tumefaciens and the Ml proteins from Mesorhizobium loti . MucR from Brucella spp. can regulate the expression of virulence genes and repress its own gene expression. Despite the well-known role played by MucR in the repression of its own gene, no target sequence has yet been identified in the mucR promoter gene...
April 2018: FEBS Open Bio
Vishnu Hosur, Michelle L Farley, Lisa M Burzenski, Leonard D Shultz, Michael V Wiles
The epidermal growth factor (EGF)-receptor ligand amphiregulin (AREG) is a potent growth factor implicated in proliferative skin diseases and in primary and metastatic epithelial cancers. AREG, synthesized as a propeptide, requires conversion to an active peptide by metalloproteases by a process known as ectodomain shedding. Although (ADAM17) a disintegrin and metalloprotease 17 is a key sheddase of AREG, ADAM8-, ADAM15-, and batimastat (broad metalloprotease inhibitor)-sensitive metalloproteases have also been implicated in AREG shedding...
April 2018: FEBS Open Bio
Martin Jabůrek, Jan Ježek, Petr Ježek
Mitochondrial uncoupling protein-2 (UCP2) mediates free fatty acid (FA)-dependent H+ translocation across the inner mitochondrial membrane (IMM), which leads to acceleration of respiration and suppression of mitochondrial superoxide formation. Redox-activated mitochondrial phospholipase A2 (mt-iPLA2γ) cleaves FAs from the IMM and has been shown to acts in synergy with UCP2. Here, we tested the mechanism of mt-iPLA2γ-dependent UCP2-mediated antioxidant protection using lipopolysaccharide (LPS)-induced pro-inflammatory and pro-oxidative responses and their acute influence on the overall oxidative stress reflected by protein carbonylation in murine lung and spleen mitochondria and tissue homogenates...
April 2018: FEBS Open Bio
Tomomi Kubota, Osamu Tani, Tomohiko Yamaguchi, Ichiji Namatame, Hitoshi Sakashita, Koji Furukawa, Kazuhiko Yamasaki
Dihydroorotate dehydrogenase (DHODH) is a flavin-binding enzyme essential for pyrimidine biosynthesis, which converts dihydroorotate to orotate. Three-dimensional structures of cytosolic DHODH of parasitic protozoa are of interest in drug discovery for neglected tropical diseases, especially because these enzymes possess significantly different structural and functional properties from the membrane-associated human enzyme. The existing crystal structures of the flavin mononucleotide (FMN)-bound DHODHs reveal a number of interactions stabilizing FMN...
April 2018: FEBS Open Bio
Yuriko Wada, Kosuke Takemura, Padmaja Tummala, Keisuke Uchida, Keisuke Kitagaki, Asuka Furukawa, Yuuki Ishige, Takashi Ito, Yukichi Hara, Takashige Suzuki, Hitomi Mimuro, Philip G Board, Yoshinobu Eishi
Infection with Helicobacter pylori is known to decrease the level of glutathione in gastric epithelial cells and increase the production of reactive oxygen species (ROS), which can lead to DNA damage and the development of gastric cancer. Cation transport regulator 1 (CHAC1) has γ-glutamylcyclotransferase activity that degrades glutathione. We found that cagA -positive H. pylori infection triggered CHAC1 overexpression in human gastric epithelial (AGS) cells leading to glutathione degradation and the accumulation of ROS...
April 2018: FEBS Open Bio
Akira Mima, Toshinori Yasuzawa, George L King, Shigeru Ueshima
Obesity is one of risk factors for chronic kidney disease (CKD), but the precise mechanism involved is unclear. This study characterizes the effect of obesity-induced glomerular inflammation, oxidative stress, and albuminuria in obese rats. Glomerular samples were collected from fatty (ZF) and lean (ZL) Zucker rats. After 2 months of feeding, body weight and albuminuria were significantly increased in ZF rats when compared to ZL rats. Expression of the inflammatory markers TNF-α and CCR2 was significantly increased in the glomeruli of ZF rats...
April 2018: FEBS Open Bio
Sun Sasaki, Manami Tsukamoto, Masaki Saito, Shintaro Hojyo, Toshiyuki Fukada, Masamichi Takami, Tatsuya Furuichi
Osteoclasts are bone-resorbing cells that play an essential role in maintaining bone homeostasis. Zinc (Zn) has been reported to inhibit osteoclast-mediated bone resorption, but the mechanism of this action has not been clarified. Zn homeostasis is tightly controlled by the coordinated actions of many Zn transporters. The Zn transporter ZIP14/Slc39a14 is involved in various physiological functions; hence, Zip14 -knockout (KO) mice exhibit multiple phenotypes. In this study, we thoroughly investigated the bone phenotypes of Zip14 -KO mice, demonstrating that the KO mice exhibited osteopenia in both trabecular and cortical bones...
April 2018: FEBS Open Bio
Hidetaka Sadanari, Kazuhiro J Fujimoto, Yuto Sugihara, Tomoki Ishida, Masaya Takemoto, Tohru Daikoku, Tsugiya Murayama
4',5,7-trihydroxy-3',5'-dimethoxyflavone (tricin), derived from Sasa albo-marginata , has been reported to suppress significantly human cytomegalovirus (HCMV) replication in human embryonic lung (HEL) fibroblast cells. However, the target protein of tricin remains unclear. This study focused on the anti-HCMV activity of tricin in terms of its binding affinity to cyclin-dependent kinase 9 (CDK9). A molecular docking study predicted that tricin binds well to the ATP-binding site of CDK9. Experimental measurements then revealed that tricin inhibits the kinase activity of CDK9 and affects the phosphorylation of the carboxy-terminal domain of RNA polymerase II...
April 2018: FEBS Open Bio
Tianyi Ma, Mi-Jeong Yoo, Tong Zhang, Lihong Liu, Jin Koh, Wen-Yuan Song, Alice C Harmon, Wei Sha, Sixue Chen
Sucrose nonfermenting 1-related protein kinase 2.6 (SnRK2.6), also known as Open Stomata 1 (OST1) in Arabidopsis thaliana , plays a pivotal role in abscisic acid (ABA)-mediated stomatal closure. Four SnRK2.6 paralogs were identified in the Brassica napus genome in our previous work. Here we studied one of the paralogs, BnSnRK2.6-2C , which was transcriptionally induced by ABA in guard cells. Recombinant BnSnRK2.6-2C exhibited autophosphorylation activity and its phosphorylation sites were mapped. The autophosphorylation activity was inhibited by S-nitrosoglutathione (GSNO) and by oxidized glutathione (GSSG), and the inhibition was reversed by reductants...
April 2018: FEBS Open Bio
Lei Yang, Yanli Wang, Shuainan Shi, Lili Xie, Tao Liu, Yuliang Wang, Hong Mu
Tumour necrosis factor alpha (TNF-α) is a multifunctional cytokine and has the capacity both to promote cell growth and to kill tumour cells by inducing cell apoptosis. However, many tumour cells develop resistance to the toxic effects of TNF-α. Thus, understanding the mechanism underlying the resistance of tumours to TNF-α toxicity and finding ways to overcome this resistance are urgently needed. In this study, we discovered that two cervical cancer cell lines, Hela and Siha, showed null responses to TNF-α cytotoxicity...
April 2018: FEBS Open Bio
Nicole M Luzi, Charles E Lyons, Darrell L Peterson, Keith C Ellis
Overproduction of cortisol by the hypothalamus-pituitary-adrenal hormone system results in the clinical disorder known as Cushing's syndrome. Genomics studies have identified a key mutation (L205R) in the α-isoform of the catalytic subunit of cAMP-dependent protein kinase (PKACα) in adrenal adenomas of patients with adrenocorticotropic hormone-independent Cushing's syndrome. Here, we conducted kinetics and inhibition studies on the L205R-PKACα mutant. We have found that the L205R mutation affects the kinetics of both Kemptide and ATP as substrates, decreasing the catalytic efficiency ( k cat / K M ) for each substrate by 12-fold and 4...
April 2018: FEBS Open Bio
Yunting Zhang, Yali Li, Yuwei He, Wenjie Hu, Yong Zhang, Xiaorong Wang, Haoru Tang
NADPH oxidase is encoded by a small gene family (Respiratory burst oxidase homologs, Rbohs ) and plays an important role in regulating various biological processes. However, little information about this gene family is currently available for strawberry. In this study, a total of seven Rboh genes were identified from strawberry through genomewide analysis. Gene structure analysis showed the number of exons ranged from 10 to 23, implying that this variation occurred in FvRboh genes by the insertion and distribution of introns; the order and approximate size of exons were relatively conserved...
April 2018: FEBS Open Bio
Borbála Vető, Pál Szabó, Caroline Bacquet, Anna Apró, Edit Hathy, Judit Kiss, János M Réthelyi, Flóra Szeri, Dávid Szüts, Tamás Arányi
5-Hydroxymethylcytosine (5hmC) is produced from 5-methylcytosine (5mC) by Ten-eleven translocation (TET) dioxygenases. The epigenetic modification 5hmC has crucial roles in both cellular development and differentiation. The 5hmC level is particularly high in the brain. While 5mC is generally associated with gene silencing/reduced expression, 5hmC is a more permissive epigenetic mark. To understand its physiological function, an easy and accurate quantification method is required. Here, we have developed a novel LC-MS/MS-based approach to quantify both genomic 5mC and 5hmC contents...
April 2018: FEBS Open Bio
Holly Stefen, Alexandra Kalyna Suchowerska, Bei Jun Chen, Merryn Brettle, Jennifer Kuschelewski, Peter William Gunning, Michael Janitz, Thomas Fath
Tropomyosins, a family of actin-associated proteins, bestow actin filaments with distinct biochemical and physical properties which are important for determining cell shape and regulating many cellular processes in eukaryotic cells. Here, we used RNA-seq to investigate the effect of four tropomyosin isoforms on gene expression in undifferentiated and differentiated rat B35 neuroblastoma cells. In undifferentiated cells, overexpression of tropomyosin isoforms Tpm1.12, Tpm2.1, Tpm3.1, and Tpm4.2 differentially regulates a vast number of genes, clustering into several gene ontology terms...
April 2018: FEBS Open Bio
Éva Csősz, Bernadett Márkus, Zsuzsanna Darula, Katalin F Medzihradszky, Judit Nemes, Emese Szabó, József Tőzsér, Csongor Kiss, Ildikó Márton
Oral squamous cell carcinoma (OSCC) is the seventh most common malignancy and the ninth most frequent cause of cancer death in Europe. Within Europe, Hungary has one of the highest rates of OSCC incidence and mortality. Thus, there is an urgent need to improve early detection. Saliva, as a readily available body fluid, became an increasingly important substance for the detection of biomarkers for many diseases. Different research groups have identified salivary biomarkers specific for OSCC for different countries...
April 2018: FEBS Open Bio
Xiangxiang Ding, Shuai Zhang, Xiao Li, Changjiang Feng, Qi Huang, Shaodong Wang, Siwei Wang, Wenjia Xia, Fan Yang, Rong Yin, Lin Xu, Mantang Qiu, Ming Li, Jun Wang
Noncoding RNA play important roles in various biological processes and diseases, including cancer. The expression profile of circular RNA (circRNA) has not been systematically investigated in lung adenocarcinoma (LUAD). In this study, we performed genomewide transcriptome profiling of coding genes, long noncoding RNA (lncRNA), and circRNA in paired LUAD and nontumor tissues by ribosomal RNA-depleted RNA sequencing. The detected reads were first mapped to the human genome to analyze expression of coding genes and lncRNA, while the unmapped reads were subjected to a circRNA prediction algorithm to identify circRNA candidates...
April 2018: FEBS Open Bio
Ke Shi, Jin-Zhong Zhang, Rui-Li Zhao, Liang Yang, Dan Guo
PSMD7, a 19S proteasome subunit, is overexpressed in most carcinoma cells. It forms a dimer with PSMD14 that functions in the removal of attached ubiquitin chain. However, there is little knowledge about the cellular mechanism of PSMD7 and its exact biological function, especially in cancer cells. In this study, we explored the role of PSMD7 in proliferation, cell cycle, apoptosis, and proteasomal proteolysis in the esophageal squamous cell carcinoma (ESCC) cell line EC9706. Our results showed that PSMD7 was highly expressed in ESCC cells...
April 2018: FEBS Open Bio
Guangwen Jin, Alkebaier Aobulikasimu, Jinying Piao, Zulipiya Aibibula, Daisuke Koga, Shingo Sato, Hiroki Ochi, Kunikazu Tsuji, Tetsuo Nakabayashi, Toshio Miyata, Atsushi Okawa, Yoshinori Asou
Osteoporosis is a progressive bone disease caused by an imbalance between bone resorption and formation. Recently, plasminogen activator inhibitor-1 (PAI-1) was shown to play an important role in bone metabolism using PAI-1-deficient mice. In this study, we evaluated the therapeutic benefits of novel, orally available small-molecule PAI-1 inhibitor (iPAI-1) in an estrogen deficiency-induced osteoporosis model. Eight-week-old C57BL/6J female mice were divided into three groups: a sham + vehicle (Sham), ovariectomy + vehicle (OVX + v), and OVX + iPAI-1 (OVX + i) group...
April 2018: FEBS Open Bio
Manida Suksawat, Anchalee Techasen, Nisana Namwat, Thianrut Boonsong, Attapol Titapun, Piti Ungarreevittaya, Puangrat Yongvanit, Watcharin Loilome
The isoform of nitric oxide synthase (NOS) found in endothelial cells (eNOS) plays a crucial role in vasodilation. We recently reported the activation of eNOS in cholangiocarcinoma (CCA) tissues and cell lines. Moreover, we also reported that the abundance of eNOS and phosphorylated eNOS (p-eNOS), as well as its upstream regulator proteins, is significantly associated with the metastatic status of CCA patients. However, the function of eNOS in CCA progression has not been addressed. Therefore, the present study aimed to investigate the function of eNOS involved in the migration and invasion ability of CCA cell lines...
April 2018: FEBS Open Bio
Jiajie Tu, Dandan Cao, Lu Li, Hoi-Hung Cheung, Wai-Yee Chan
Induced pluripotent stem cells (iPSCs) are useful for modeling neuron development and related diseases. Cortical interneurons are essential players in neuropsychiatric diseases such as autism. miRNAs are a class of pivotal regulators in neural differentiation. Using a previously established model of cortical interneuron differentiation from human embryonic stem cells, we profiled miRNAs involved in differentiation from human iPSCs. A number of miRNAs were modulated in the differentiation process. This study captured the temporal in vitro neurogenesis from iPSCs to mature cortical interneurons...
April 2018: FEBS Open Bio
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