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FEBS Open Bio

Chiharu Moriya, Kohzoh Imai, Hiroaki Taniguchi
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive and lethal cancer that is typically diagnosed at a later stage with metastases and is difficult to treat. Therefore, investigating the mechanism of PDAC initiation is important to aid early-stage cancer detection. PRDM14 is a transcription factor that maintains pluripotency in embryonic stem cells and is overexpressed in several cancers. We previously reported that PRDM14 is overexpressed and regulates cancer stem-like phenotypes in PDAC, and herein, we assess whether PRDM14 expression increases prior to tumorigenesis...
October 2018: FEBS Open Bio
Zunqiang Yan, Tiantuan Jiang, Pengfei Wang, Xiaoyu Huang, Qiaoli Yang, Wenyang Sun, Shuangbao Gun
Clostridium perfringens type C is a pathogen that causes necrotizing enteritis (NE), which is an intestinal tract disease in piglets. The pathogenesis of C. perfringens type C-induced NE is still unclear, leading to a lack of effective therapies. Earlier studies have reported that circular RNAs (circRNAs) are involved in the pathogenic processes of various diseases. However, it is not known if circRNAs in spleen play a role in C. perfringens type C infection in NE. To address this question, we infected 7-day-old piglets with C...
October 2018: FEBS Open Bio
Wei Zhu, Lanfen Li, Mingjing Deng, Bo Wang, Mengfei Li, Guofang Ding, Zuisu Yang, Dan Medynski, Xiaotao Lin, Ying Ouyang, Jirui Lin, Luyuan Li, Xinli Lin
Native α1-antitrypsin (AAT) is a 52-kDa glycoprotein that acts as an antiprotease and is the physiological inhibitor of neutrophil serine proteases. The main function of AAT is to protect the lung from proteolytic damage induced by inflammation. AAT deficiency (AATD) is a codominant autosomal disorder caused by pathogenic mutations in SERPINA1 gene, leading to reduced levels of serum AAT. The deficiency is known to increase the risk of pulmonary emphysema and chronic obstructive pulmonary disease as a consequence of proteolytic imbalance induced by inflammation, associated in many instances with cigarette smoking and other environmental hazards...
October 2018: FEBS Open Bio
Xian-Yang Qin, Jun Lu, Muyi Cai, Soichi Kojima
We previously reported a profound augmentation in the hepatic levels of a pro-inflammatory precursor, arachidonic acid (AA), during liver tumorigenesis. Here, we report a critical role of the induced reactive oxygen species (ROS)-mediated cellular activation of a protein cross-linking enzyme, transglutaminase 2 (TG2), in liver injury by AA. In cultures of hepatic cells, AA dose-dependently suppressed cell growth, which accompanied the induced nuclear accumulation of TG2, as demonstrated in EGFP-tagged, TG2-overexpressing hepatic cells...
October 2018: FEBS Open Bio
Keisuke Tanaka, Naoko Teramura, Osamu Hayashida, Katsumasa Iijima, Teru Okitsu, Shunji Hattori
The collagenase secreted by Grimontia hollisae strain 1706B is a 74 kDa protein that consists of two parts: the catalytic module and a C-terminal segment that includes the bacterial pre-peptidase C-terminal domain. Here, we produced a recombinant C-terminal segment protein and examined its ability to bind collagen and other characteristics as compared with collagen-binding domains (CBDs) derived from Hathewaya histolytica ( Clostridium histolyticum ) collagenases; these CBDs are the only ones thus far identified in bacterial collagenases...
October 2018: FEBS Open Bio
Prajna Tripathi, Priyanka Parijat, Virendra Kumar Patel, Janendra K Batra
Mycobacterium tuberculosis ( Mtb ) is known to persist in extremely hostile environments within host macrophages. The ability to withstand such proteotoxic stress comes from its highly conserved molecular chaperone machinery. ClpB, a unique member of the AAA+ family of chaperones, is responsible for resolving aggregates in Mtb and many other bacterial pathogens. Mtb produces two isoforms of ClpB, a full length and an N-terminally truncated form (ClpB∆N), with the latter arising from an internal translation initiation site...
October 2018: FEBS Open Bio
Xiaodong Xie, Qingshan Ni, Daxue Zhou, Ying Wan
Inflammatory bowel disease (IBD) is a multifactorial disease involving defective immune responses against invasive microbiota. Genes associated with innate immune responses to microbes have been highlighted in the pathogenesis of IBD. To determine the role of Rab32 in the pathogenesis of IBD, we administered dextran sodium sulfate (DSS) to CD11c+ cell-specific Rab32 knockout ( CD11c -Cre+ Rab32f/f ) mice to induce colitis. Rab32 deficiency in CD11c+ cells resulted in more severe disease progression and increased mortality...
October 2018: FEBS Open Bio
Sercan Karav, Giorgio Casaburi, Steven A Frese
Mucin glycoproteins play an important role in protecting the gut epithelium by keeping gut microbes from direct contact with the gut epithelium while allowing for diffusion of small molecules from the lumen to the epithelium. The mucin glycocalyx can be degraded by gut bacteria such as Bacteroides and Akkermansia , but other bacteria, such as Bifidobacterium longum subsp . Infantis , cannot consume mucin glycans. Untargeted mass spectrometry profiles were compared to microbiome profiles to assess how different gut microbiomes affect colonic mucin degradation...
October 2018: FEBS Open Bio
Stève de Bossoreille, Patrice Morel, Christophe Trehin, Ioan Negrutiu
The nucleoplasm and nucleolus are the two main territories of the nucleus. While specific functions are associated with each of these territories (such as mRNA synthesis in the nucleoplasm and ribosomal rRNA synthesis in the nucleolus), some proteins are known to be located in both. Here, we investigated the molecular function of REBELOTE (RBL), an Arabidopsis thaliana protein previously characterized as a regulator of floral meristem termination. We show that RBL displays a dual localization, in the nucleolus and nucleoplasm...
October 2018: FEBS Open Bio
Elise Roussel, Marie-Claude Drolet, Anne-Marie Lavigne, Marie Arsenault, Jacques Couet
Cardiac hypertrophy (CH) is an important and independent predictor of morbidity and mortality. Through expression profiling, we recently identified a subset of genes ( Dhrs7c , Decr , Dhrs11 , Dhrs4 , Hsd11b1 , Hsd17b10 , Hsd17b8 , Blvrb , Pecr ), all of which are members of the short-chain dehydrogenase/reductase (SDR) superfamily and are highly expressed in the heart, that were significantly dysregulated in a rat model of CH caused by severe aortic valve regurgitation (AR). Here, we studied their expression in various models of CH, as well as factors influencing their regulation...
October 2018: FEBS Open Bio
Peng Jing, Jiaqiong Zou, Kegui Weng, Pei Peng
Previous studies have reported excessive expression of apoptosis-antagonizing transcription factor (AATF) in various tumors, where it reinforces the generation and development of cancers and is linked to the clinical outcome. Nevertheless, the expression and influence of AATF in Wilms' tumor (WT) is largely unknown. Here, we discovered that AATF expression was markedly increased in WT tissues as compared to the surrounding normal tissues. Elevated levels of AATF expression were related to tumor relapse and pulmonary metastasis, congruent with it being a predictor of clinical outcome in people suffering from WT...
October 2018: FEBS Open Bio
Natacha Pérébaskine, Stéphane Thore, Sébastien Fribourg
Rrp5 is an essential factor during the ribosome biogenesis process. The protein contains a series of 12 S1 RNA-binding domains followed by a TetratricoPeptide Repeat (TPR) domain. In the past, several studies aiming at defining the function of the TPR domain have used nonequivalent Rrp5 constructs, as these protein fragments include not only the TPR module, but also three or four S1 domains. We solved the structure of the Rrp5 TPR module and demonstrated in vitro that the TPR region alone does not bind RNA, while the three S1 domains preceding the TPR module can associate with homopolymeric RNA...
October 2018: FEBS Open Bio
Qingmiao Zhang, Xinqun Chai, Feitao Deng, Weixiang Ouyang, Ting Song
Hyperglycemia during pregnancy is associated with fetal lung development disorders and surfactant protein (SP) deficiency. Here, we examined the role of FOXA2 and Akt signaling in fetal lung development during diabetic pregnancy. Sprague-Dawley rats were injected with streptozocin (STZ) during pregnancy to induce diabetes (DM). DM-exposed fetal lungs exhibited reduced numbers of alveoli, irregularities in the appearance and thickness of the alveolar septum, increased levels of glycogen and lipids in type II alveolar epithelial cells, fewer microvilli and mature lamellar bodies, and swollen mitochondria...
October 2018: FEBS Open Bio
Kyeong-Lok Park, Da-Gyo Oh, Young-Ok Kim, Kyeong-Seob Song, Do-Whan Ahn
Receptor activator of nuclear factor-κB ligand (RANKL) is required for initiation of osteoclastogenesis, with the signaling pathway including the NF-kB, c-Fos, and nuclear factor of activated T cells, cytoplasmic 1 (NFATc1) transcription factors. Because NFATc1 expression is autoamplified, we investigated the molecular mechanism by which peroxisome proliferator-activated receptor gamma (PPARγ) activation by the thiazolidinedione drug rosiglitazone decreases NFATc1 expression during RANKL stimulation. Western blotting demonstrated that rosiglitazone attenuated the increase in NFATc1 protein level induced by RANKL without affecting that of PPARγ...
October 2018: FEBS Open Bio
Xia An, Guanrong Jin, Jingyu Zhang, Xiahong Luo, Changli Chen, Wenlue Li, GuangYing Ma, Liang Jin, Lunjin Dai, Xiaohua Shi, Wei Wei, Guanlin Zhu
The molecular mechanisms that underlie drought stress responses in kenaf, an important crop for the production of natural fibers, are poorly understood. To address this issue, we describe here the first iTRAQ-based comparative proteomic analysis of kenaf seedlings. Plants were divided into the following three treatment groups: Group A, watered normally (control); Group B, not watered for 6 days (drought treatment); and Group C, not watered for 5 days and then rewatered for 1 day (recovery treatment). A total of 5014 proteins were detected, including 4932 (i...
October 2018: FEBS Open Bio
Wakana Iwasaki, Yuta Miya, Naoki Horikoshi, Akihisa Osakabe, Hiroyuki Taguchi, Hiroaki Tachiwana, Takehiko Shibata, Wataru Kagawa, Hitoshi Kurumizaka
[This corrects the article DOI: 10.1016/j.fob.2013.08.007.].
September 2018: FEBS Open Bio
Inger K Olsbu, Giorgio Zoppellaro, K Kristoffer Andersson, Jean-Luc Boucher, Hans-Petter Hersleth
Nitric oxide (NO) produced by mammalian nitric oxide synthases (mNOSs) is an important mediator in a variety of physiological functions. Crystal structures of mNOSs have shown strong conservation of the active-site residue Val567 (numbering for rat neuronal NOS, nNOS). NOS-like proteins have been identified in several bacterial pathogens, and these display striking sequence identity to the oxygenase domain of mNOS (NOSoxy), with the exception of a Val to Ile mutation at the active site. Preliminary studies have highlighted the importance of this Val residue in NO-binding, substrate recognition, and oxidation in mNOSs...
September 2018: FEBS Open Bio
Li Liao, Xiaoyu Ji, Mengxi Ge, Qiong Zhan, Ruofan Huang, Xiaohua Liang, Xinli Zhou
Brain metastasis (BM) is the primary contributor to mortality in non-small cell lung cancer (NSCLC) patients. Although the findings of NSCLC genetic sequencing studies suggest the potential for personalizing therapeutic approaches, the genetic profiles and underlying mechanisms of BM progression remain poorly understood. Here, we investigated the genetic profiles of brain metastases from NSCLC in six patients with primary tumors and corresponding BM samples via whole exome sequencing and targeted panel sequencing...
September 2018: FEBS Open Bio
Shotaro Kamata, Junya Yamamoto, Haruka Ohtani, Yuka Tosaka, Sayumi Yoshikawa, Noriyuki Akahoshi, Isao Ishii
Overnight fasting is a routine procedure before surgery in clinical settings. Intermittent fasting is the most common diet/fitness trend implemented for weight loss and the treatment of lifestyle-related diseases. In either setting, the effects not directly related to parameters of interest, either beneficial or harmful, are often ignored. We previously demonstrated differential activation of cellular adaptive responses in 13 atrophied/nonatrophied organs of fasted mice by quantitative PCR analysis of gene expression...
September 2018: FEBS Open Bio
GaoFeng Hu, QianWen Lv, JiaXiu Yan, LiJun Chen, Juan Du, Ke Zhao, Wei Xu
Integrated studies of accumulated data can be performed to obtain more reliable information and more feasible measures for investigating potential diagnostic biomarkers of gastric cancer (GC) and to explore related molecular mechanisms. This study aimed to identify microRNAs involved in GC by integrating data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus. Through our analysis, we identified hsa-miR-17 (miR-17) as a suitable candidate. We performed a meta-analysis of published studies and analyzed clinical data from TCGA to evaluate the clinical significance and diagnostic value of miR-17 in GC...
September 2018: FEBS Open Bio
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