Darija Putar, Anja Čizmar, Xiaoting Chao, Marija Šimić, Marko Šoštar, Tamara Ćutić, Lucija Mijanović, Ana Smolko, Hui Tu, Pierre Cosson, Igor Weber, Huaqing Cai, Vedrana Filić
RasG is a major regulator of macropinocytosis in Dictyostelium discoideum . Its activity is under the control of an IQGAP-related protein, IqgC, which acts as a RasG-specific GAP (GTPase activating protein). IqgC colocalizes with the active Ras at the macropinosome membrane during its formation and for some time after the cup closure. However, the loss of IqgC induces only a minor enhancement of fluid uptake in axenic cells that already lack another RasGAP, NF1. Here, we show that IqgC plays an important role in the regulation of macropinocytosis in the presence of NF1 by restricting the size of macropinosomes...
January 2024: Open Biology