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BioResearch Open Access

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https://www.readbyqxmd.com/read/27872794/in-silico-identification-of-potential-peptides-or-allergen-shot-candidates-against-aspergillus-fumigatus
#1
Raman Thakur, Jata Shankar
Aspergillus fumigatus is capable of causing invasive aspergillosis or acute bronchopulmonary aspergillosis, and the current situation is alarming. There are no vaccine or allergen shots available for Aspergillus-induced allergies. Thus, a novel approach in designing of an effective vaccine or allergen shot candidate against A. fumigatus is needed. Using immunoinformatics approaches from the characterized A. fumigatus allergens, we have mapped epitopic regions to predict potential peptides that elicit both Aspergillus-specific T cells and B cell immune response...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27872793/improved-methods-to-produce-tissue-engineered-skin-substitutes-suitable-for-the-permanent-closure-of-full-thickness-skin-injuries
#2
Danielle Larouche, Laurence Cantin-Warren, Maxime Desgagné, Rina Guignard, Israël Martel, Akram Ayoub, Amélie Lavoie, Robert Gauvin, François A Auger, Véronique J Moulin, Lucie Germain
There is a clinical need for skin substitutes to replace full-thickness skin loss. Our group has developed a bilayered skin substitute produced from the patient's own fibroblasts and keratinocytes referred to as Self-Assembled Skin Substitute (SASS). After cell isolation and expansion, the current time required to produce SASS is 45 days. We aimed to optimize the manufacturing process to standardize the production of SASS and to reduce production time. The new approach consisted in seeding keratinocytes on a fibroblast-derived tissue sheet before its detachment from the culture plate...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27843709/a-computational-model-of-cellular-engraftment-on-lung-scaffolds
#3
Joshua J Pothen, Vignesh Rajendran, Darcy Wagner, Daniel J Weiss, Bradford J Smith, Baoshun Ma, Jason H T Bates
The possibility that stem cells might be used to regenerate tissue is now being investigated for a variety of organs, but these investigations are still essentially exploratory and have few predictive tools available to guide experimentation. We propose, in this study, that the field of lung tissue regeneration might be better served by predictive tools that treat stem cells as agents that obey certain rules of behavior governed by both their phenotype and their environment. Sufficient knowledge of these rules of behavior would then, in principle, allow lung tissue development to be simulated computationally...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27843708/prospective-preliminary-in-vitro-investigation-of-a-magnetic-iron-oxide-nanoparticle-conjugated-with-ligand-cd80-and-vegf-antibody-as-a-targeted-drug-delivery-system-for-the-induction-of-cell-death-in-rodent-osteosarcoma-cells
#4
AnneMarie Kay Kovach, Jen M Gambino, Vina Nguyen, Zach Nelson, Taylor Szasz, Jun Liao, Lakiesha Williams, Sandra Bulla, Raj Prabhu
Target drug deliveries using nanotechnology are a novel consideration in the treatment of cancer. We present herein an in vitro mouse model for the preliminary investigation of the efficacy of an iron oxide nanoparticle complex conjugated to vascular endothelial growth factor (VEGF) antibody and ligand cluster of differentiation 80 (CD80) for the purpose of eventual translational applications in the treatment of human osteosarcoma (OSA). The 35 nm diameter iron oxide magnetic nanoparticles are functionalized with an n-hydroxysuccinimide biocompatible coating and are conjugated on the surface to proteins VEGF antibody and ligand CD80...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27843707/fresh-and-frozen-tissue-engineered-three-dimensional-bone-ligament-bone-constructs-for-sheep-anterior-cruciate-ligament-repair-following-a-2-year-implantation
#5
Vasudevan Mahalingam, Edward M Wojtys, Deneen M Wellik, Ellen M Arruda, Lisa M Larkin
Injuries to the anterior cruciate ligament (ACL) often require surgical reconstruction utilizing tendon grafts to restore knee function and stability. Some current graft options for ACL repair are associated with poor long-term outcomes. Our laboratory has fabricated tissue-engineered bone-ligament-bone (BLB) constructs that demonstrate native ligament regeneration and advancement toward native ACL mechanical properties in a sheep ACL reconstruction model. Prior work has shown that freezing BLBs as a method of preservation resulted in similar outcomes compared with fresh BLBs after 6-month implantation...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27790385/role-of-the-microbiota-in-colorectal-cancer-updates-on-microbial-associations-and-therapeutic-implications
#6
REVIEW
Olivia I Coleman, Tiago Nunes
Genetic, environmental, and dietary factors have been found to influence the development and progression of colorectal cancer (CRC). More recently, accumulating evidence associates the intestinal microbiota with the initiation and progression of this disease. While studies have shown that individuals with CRC display alterations in gut bacterial composition, it remains somewhat unclear whether such differences drive cancer development or whether they are a response to tumorigenesis. In this review, the authors assess new evidence linking the community structure or specific bacterial factors of the intestinal microbiota to CRC development and progression, with insights into therapeutic implications...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27679753/cytokine-rich-adipose-tissue-extract-production-from-water-assisted-lipoaspirate-methodology-for-clinical-use
#7
Jenny Lopez, Outi Huttala, Jertta-Riina Sarkanen, Ilkka Kaartinen, Hannu Kuokkanen, Timo Ylikomi
Proper functioning wound healing strategies are sparse. Adequate vascular formation to the injured area, as well as replacement of the volume loss, is fundamental in soft tissue repair. Tissue engineering strategies have been proposed for the treatment of these injury sites. Novel cell-free substance, human adipose tissue extract (ATE), has been previously shown to induce in vitro angiogenesis and adipogenesis and in vivo soft tissue formation. This study reports the translation of ATE preparation from laboratory to the operating room (OR)...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27668131/safety-and-treatment-effectiveness-of-a-single-autologous-protein-solution-injection-in-patients-with-knee-osteoarthritis
#8
Rogier A M van Drumpt, Walter van der Weegen, William King, Krista Toler, Mitchell M Macenski
Osteoarthritis (OA) is a common degenerative condition characterized by pain and loss of function. A pathological biochemical environment with excess inflammatory and catabolic proteins is a major contributor to OA. nSTRIDE(®) Autologous Protein Solution (APS) is a new therapy under development for the treatment of OA. This therapy is formed from a patient's blood and contains high concentrations of anti-inflammatory and anabolic proteins. This study assessed the safety and treatment effects of APS. Eleven subjects with early to moderate OA were injected with APS...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27610271/functional-effects-of-delivering-human-mesenchymal-stem-cell-seeded-biological-sutures-to-an-infarcted-heart
#9
Katrina J Hansen, John T Favreau, Jacques P Guyette, Ze-Wei Tao, Spencer T Coffin, Anny Cunha-Gavidia, Brian D'Amore, Luke R Perreault, John P Fitzpatrick, Angelica DeMartino, Glenn R Gaudette
Stem cell therapy has the potential to improve cardiac function after myocardial infarction (MI); however, existing methods to deliver cells to the myocardium, including intramyocardial injection, suffer from low engraftment rates. In this study, we used a rat model of acute MI to assess the effects of human mesenchymal stem cell (hMSC)-seeded fibrin biological sutures on cardiac function at 1 week after implant. Biological sutures were seeded with quantum dot (Qdot)-loaded hMSCs for 24 h before implantation...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27610270/hepatic-differentiation-of-human-induced-pluripotent-stem-cells-in-a-perfused-three-dimensional-multicompartment-bioreactor
#10
Nora Freyer, Fanny Knöspel, Nadja Strahl, Leila Amini, Petra Schrade, Sebastian Bachmann, Georg Damm, Daniel Seehofer, Frank Jacobs, Mario Monshouwer, Katrin Zeilinger
The hepatic differentiation of human induced pluripotent stem cells (hiPSC) holds great potential for application in regenerative medicine, pharmacological drug screening, and toxicity testing. However, full maturation of hiPSC into functional hepatocytes has not yet been achieved. In this study, we investigated the potential of a dynamic three-dimensional (3D) hollow fiber membrane bioreactor technology to improve the hepatic differentiation of hiPSC in comparison to static two-dimensional (2D) cultures. A total of 100 × 10(6) hiPSC were seeded into each 3D bioreactor (n = 3)...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27610269/association-of-visual-acuity-and-cognitive-impairment-in-older-individuals-fujiwara-kyo-eye-study
#11
Masashi Mine, Kimie Miyata, Masayuki Morikawa, Tomo Nishi, Nozomi Okamoto, Ryo Kawasaki, Hidetoshi Yamashita, Norio Kurumatani, Nahoko Ogata
Both visual impairment and cognitive impairment are essential factors that determine the quality of life in the aged population. The aim of this study was to determine if a correlation existed between visual acuity and cognitive impairment in an elderly Japanese population. The Fujiwara-kyo Eye Study was a cross-sectional study of individuals aged ≥68 years who lived in Nara Prefecture of Japan. Participants underwent ophthalmological examinations and cognitive function test. A mild visual impairment was defined as having a best corrected visual acuity (BCVA) >0...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27610268/recent-applications-of-coaxial-and-emulsion-electrospinning-methods-in-the-field-of-tissue-engineering
#12
REVIEW
Phillip McClellan, William J Landis
Electrospinning has emerged as an effective method of producing nanoscale fibers for use in multiple fields of study. One area of significant interest is nanofiber utilization for tissue engineering because the nanofibrous mats can mimic the native extracellular matrix of biological tissues. A logical next step is the inclusion of certain molecules and compounds to accelerate or increase the efficacy of tissue regeneration. Two methods are under scrutiny for their capability to encapsulate therapeutic compounds within electrospun nanofibers: emulsion and coaxial electrospinning...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27588220/in-vitro-testing-of-biomaterials-for-neural-repair-focus-on-cellular-systems-and-high-content-analysis
#13
Vito Antonio Baldassarro, Luisa Stella Dolci, Chiara Mangano, Luciana Giardino, Chiara Gualandi, Maria Letizia Focarete, Laura Calzà
Biomimetic materials are designed to stimulate specific cellular responses at the molecular level. To improve the soundness of in vitro testing of the biological impact of new materials, appropriate cell systems and technologies must be standardized also taking regulatory issues into consideration. In this study, the biological and molecular effects of different scaffolds on three neural systems, that is, the neural cell line SH-SY5Y, primary cortical neurons, and neural stem cells, were compared. The effect of poly(L-lactic acid) scaffolds having different surface geometry (conventional two-dimensional seeding flat surface, random or aligned fibers as semi3D structure) and chemical functionalization (laminin or ECM extract) were studied...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27588219/regeneration-of-cartilage-in-human-knee-osteoarthritis-with-autologous-adipose-tissue-derived-stem-cells-and-autologous-extracellular-matrix
#14
Jaewoo Pak, Jung Hun Lee, Kwang Seung Park, Byeong Chul Jeong, Sang Hee Lee
This clinical case series demonstrates that percutaneous injections of autologous adipose tissue-derived stem cells (ADSCs) and homogenized extracellular matrix (ECM) in the form of adipose stromal vascular fraction (SVF), along with hyaluronic acid (HA) and platelet-rich plasma (PRP) activated by calcium chloride, could regenerate cartilage-like tissue in human knee osteoarthritis (OA) patients. Autologous lipoaspirates were obtained from adipose tissue of the abdominal origin. Afterward, the lipoaspirates were minced to homogenize the ECM...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27500014/inadequate-processing-of-decellularized-dermal-matrix-reduces-cell-viability-in-vitro-and-increases-apoptosis-and-acute-inflammation-in-vivo
#15
Aaron H Morris, Julie Chang, Themis R Kyriakides
Decellularized tissue scaffolds are commonly used in the clinic because they can be used as substitutes for more traditional biomaterials, while imparting additional physiological effects. Nevertheless, reports of complications associated with their use are widespread and poorly understood. This study probes possible causes of these complications by examining cell viability and apoptosis in response to eluents from decellularized dermis. Using multiple sources of decellularized dermis, this study shows that typical decellularized scaffolds (prepared with commonly used laboratory techniques, as well as purchased from commercial sources) contain soluble components that are cytotoxic and that these components can be removed by extensive washes in cell culture media...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27493841/persistence-of-neutralizing-antibody-against-dengue-virus-2-after-70-years-from-infection-in-nagasaki
#16
Mya Myat Ngwe Tun, Yoshihito Muta, Shingo Inoue, Kouichi Morita
This study aimed to investigate the duration of humoral immune responses to dengue virus (DENV) infection in Japanese who experienced acute febrile illness with hemorrhagic manifestations 70 years ago, when an epidemic of dengue occurred in Nagasaki, Japan, from 1942 to 1944. A Japanese volunteer requested serological diagnosis of DENV infection in 2014 and donated blood sample to measure the antibody titer against DENV by antiflavi IgG indirect ELISA, focus reduction neutralization test, and plaque reduction neutralization test...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27403405/pathogenesis-of-zika-virus-associated-embryopathy
#17
Anthony R Mawson
A strong causal association has become evident between Zika virus (ZIKV) infection during pregnancy and the occurrence of fetal growth restriction, microcephaly and eye defects. Circumstantial evidence is presented in this paper in support of the hypothesis that these effects, as well as the Guillain-Barré syndrome, are due to an endogenous form of hypervitaminosis A resulting from ZIKV infection-induced damage to the liver and the spillage of stored vitamin A compounds ("retinoids") into the maternal and fetal circulation in toxic concentrations...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27354930/efficacy-of-intraperitoneal-administration-of-pegylated-nell-1-for-bone-formation
#18
Justine Tanjaya, Yulong Zhang, Soonchul Lee, Jiayu Shi, Eric Chen, Pia Ang, Xinli Zhang, Sotirios Tetradis, Kang Ting, Benjamin Wu, Chia Soo, Jin Hee Kwak
Systemically delivered NEL-like molecule-1 (NELL-1), a potent pro-osteogenic protein, promotes bone formation in healthy and osteoporotic mouse models. PEGylation of NELL-1 (NELL-PEG) increases the half-life of the protein in a mouse model without compromising its osteogenic potential, thereby improving its pharmacokinetics upon systemic delivery. This study consists of a twofold approach: a biodistribution test and an in vivo osteogenic potential test. The biodistribution test compared two commonly used administration methods for drug delivery other than intravenous-intraperitoneal (IP) and subcutaneous (SC)-to examine NELL-PEG biodistribution in mice...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27298756/serum-levels-of-growth-differentiation-factor-11-are-independently-associated-with-low-hemoglobin-values-in-hemodialysis-patients
#19
Sho-Ichi Yamagishi, Takanori Matsui, Yuka Kurokawa, Kei Fukami
Circulating levels of growth differentiation factor 11 (GDF11) have been shown to decrease with age in several mammalian species, and supplementation of GDF11 by heterochronic parabiosis or systemic administration reverses age-related organ damage. However, there is some controversy about the pathophysiological role of GDF11 in aging-associated organ damage. Since aging process is accelerated in uremia, we compared serum levels of GDF11 in hemodialysis (HD) patients with those in age-matched healthy controls, and then determined the independent clinical correlates of GDF11 in HD subjects...
2016: BioResearch Open Access
https://www.readbyqxmd.com/read/27298755/impact-of-storage-at-80%C3%A2-c-on-encapsulated-liver-spheroids-after-liquid-nitrogen-storage
#20
Peter Kilbride, Jordi Gonzalez-Molina, Natasha Maurmann, Joana Mendonça da Silva, Stephanie Gibbons, Clare Selden, Barry Fuller, John Morris
For many bioengineered tissues to have practical clinical application, cryopreservation for use on demand is essential. This study examined different thermal histories on warming and short holding periods at different subzero temperatures on subsequent functional recoveries of alginate encapsulated liver spheroids (ELS) for use in a bioartificial liver device. This mimicked transport at liquid nitrogen (-196°C) or dry ice (∼-80°C) temperatures. Holding at -80°C on warming after -196°C storage resulted in ELS expressing significant (p < 0...
2016: BioResearch Open Access
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