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Journal of Analytical Methods in Chemistry

Muhammad Alamzeb, Muhammad Omer, Mamoon Ur-Rashid, Muslim Raza, Saqib Ali, Behramand Khan, Asad Ullah
Urease enzyme is responsible for gastric cancer, peptic ulcer, hepatic coma, and urinary stones in millions of people across the world. So, there is a strong need to develop new and safe antiurease drugs, particularly from natural sources. In search for new and effective drugs from natural sources bioassay-guided fractionation and isolation of Berberis glaucocarpa Stapf roots bark resulted in the isolation and characterization, on the basis of 1D and 2D NMR data, of two bisbenzylisoquinoline alkaloids, oxyacanthine ( 1 ) and tetrandrine ( 2 ), followed by urease inhibition studies...
2018: Journal of Analytical Methods in Chemistry
Sami Bawazeer, Khalid M Badr El-Din, Ahmed M Abdel-Megied
A simple, rapid, and validated UPLC method was developed for the simultaneous quantitation of paracetamol (PAR), tizanidine (TIZ), aceclofenac (ACF), and nimesulide (NIM) either in pure forms or in their different tablet dosage forms. Chromatographic separation was attained on an ACQUITY UPLC™ BEH C18 column (100 mm × 2.1 mm, 1.7  μ m) with a mobile phase consisting of 20 mM phosphate buffer (pH 7.0) : acetonitrile in the proportion (60 : 40 v / v ) isocratically pumped at a flow rate of 1...
2018: Journal of Analytical Methods in Chemistry
Wenjuan Cui, Qin Liu, Shan Xiong, Lujun Qiao
A simple, sensitive, and accurate LC-MS/MS method was established and validated for the simultaneous quantification of dexmedetomidine, dezocine, and midazolam in rat plasma. Chromatographic separation was achieved on a C18 column (50 mm × 2.1 mm, 3  µ m) using a mobile phase composed of water (containing 0.1% formic acid) and acetonitrile. The lower limits of quantification were 0.1, 0.1, and 0.2 ng/mL for dexmedetomidine, dezocine, and midazolam in rat plasma, respectively. The analytes were determined with selected reaction monitoring under positive ionization mode...
2018: Journal of Analytical Methods in Chemistry
Morteza Shahrestani, Mohammad Saber Tehrani, Shahram Shoeibi, Parviz Aberoomand Azar, Syed Waqif Husain
The primary aromatic amines (PAAs) are food contaminants which may exist in packaged food. Polyurethane (PU) adhesives which are used in flexible packaging are the main source of PAAs. It is the unreacted diisocyanates which in fact migrate to foodstuff and then hydrolyze to PAAs. These PAAs include toluenediamines (TDAs) and methylenedianilines (MDAs), and the selected PAAs were 2,4-TDA, 2,6-TDA, 4,4'-MDA, 2,4'-MDA, and 2,2'-MDA. PAAs have genotoxic, carcinogenic, and allergenic effects. In this study, extraction methods were applied on a 3% acetic acid as food simulant which was spiked with the PAAs under study...
2018: Journal of Analytical Methods in Chemistry
Wenbo Wang, Linlin Zhao, Huiyong Huang, Jiamei Yao, Lu Zhou, Dongsheng Wang, Xinjian Qiu
A rapid, accurate, and sensitive ultra-high performance liquid chromatography (UHPLC) method was established for simultaneously detecting naringin, hesperidin, neohesperidin, meranzin hydrate, naringenin, and hesperetin in Fructus aurantii (FA) decoction. Analysis was performed on Waters BEH (R) C18 (50 mm × 2.1 mm, 1.7  μ m) at a flow rate of 0.2 mL/min by using (A) acetonitrile and (B) 0.5% acetic acid-water as the mobile phase. The method was well validated on linearity, precision, recoveries, and stability...
2018: Journal of Analytical Methods in Chemistry
G B Slepchenko, T M Gindullina, M A Gavrilova, A Zh Auelbekova
For the first time, the possibility of using stripping voltammetry for the simultaneous determination of aflatoxins В1 and М1 on a glassy-carbon electrode has been shown. The influence of various factors ( Е э , τ э , w , and the nature of the background electrolyte) on the potential and magnitude of the oxidation current of mycotoxins has been estimated. Working conditions for voltammetric determination and reproducibility of analytical signals for two mycotoxins have been selected. The mutual influence of aflatoxins В1 and М1 on the value of analytical signals in their simultaneous presence has been studied...
2018: Journal of Analytical Methods in Chemistry
Sang A Park, Soi Jeong, Young Ho Choe, Young-Min Hyun
Increase in vascular permeability is a conclusive response in the progress of inflammation. Under controlled conditions, leukocytes are known to migrate across the vascular barriers to the sites of inflammation without severe vascular rupture. However, when inflammatory state becomes excessive, the leakage of blood components may occur and can be lethal. Basically, vascular permeability can be analyzed based on the intensity of blood outflow. To evaluate the amount and rate of leakage in live mice, we performed cremaster muscle exteriorization to visualize blood flow and neutrophil migration...
2018: Journal of Analytical Methods in Chemistry
Nguyen Phuong Thao, Pham Thanh Binh, Nguyen Thi Luyen, Ta Manh Hung, Nguyen Hai Dang, Nguyen Tien Dat
As part of an ongoing search for new natural products from medicinal plants to treat type 2 diabetes, two new compounds, a megastigmane sesquiterpenoid sulfonic acid ( 1 ) and a new cyclohexylethanoid derivative ( 2 ), and seven related known compounds ( 3-9 ) were isolated from the leaves of Wedelia chinensis (Osbeck.) Merr. The structures of the compounds were conducted via interpretation of their spectroscopic data (1D and 2D NMR, IR, and MS), and the absolute configurations of compound 1 were determined by the modified Mosher's method...
2018: Journal of Analytical Methods in Chemistry
Sabriye Yusan, Mokhlesur M Rahman, Nasir Mohamad, Tengku M Arrif, Ahmad Zubaidi A Latif, Mohd Aznan M A, Wan Sani B Wan Nik
A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibration plot is obtained over a wide concentration range of glucose from 1 × 10-5 to 2 × 10-3  M. Under optimal conditions, the biosensor exhibits high sensitivity (0...
2018: Journal of Analytical Methods in Chemistry
Ya-Li Wang, Guang Hu, Qian Zhang, Yu-Xiu Yang, Qiao-Qiao Li, Yuan-Jia Hu, Hua Chen, Feng-Qing Yang
Tyrosinase (TYR) is a rate-limiting enzyme in the synthesis of melanin, while direct TYR inhibitors are a class of important clinical antimelanoma drugs. This study established a spectrum-effect relationship analysis method and high-performance liquid chromatography-mass spectrometry (LC-MS) analysis method to screen and identify the active ingredients that inhibited TYR in Salvia miltiorrhiza - Carthamus tinctorius (Danshen-Honghua, DH) herbal pair. Seventeen potential active compounds (peaks) in the extract of DH herbal pair were predicted, and thirteen of them were tentatively identified by LC-MS analysis...
2018: Journal of Analytical Methods in Chemistry
Mengsi Cao, Pingping Zhang, Yanru Feng, Huayin Zhang, Huaijiao Zhu, Kaoqi Lian, Weijun Kang
A reliable derivatization method has been developed to detect and quantify morpholine in apple juices and ibuprofen with gas chromatography-mass spectrometry. Morpholine can react with sodium nitrite under acidic condition to produce stable and volatile N-nitrosomorpholine derivative. In this experiment, various factors affecting the derivatization and extraction process were optimized, including volume and concentration of hydrochloric acid, quantity of sodium nitrite, derivatization temperature, derivatization time, extraction reagents, and extraction time...
2018: Journal of Analytical Methods in Chemistry
Lele Li, Yang Wang, Yang Xiu, Shuying Liu
Two quantitative methods (-ESI full scan and -ESI PRM MS) were developed to analyze ginsenosides in ginseng stem-leaf by using UPLC-Q-Exactive Orbitrap/MS. By means of -ESI PRM MS method, the contents of eighteen ginsenosides in Asian ginseng stem-leaf (ASGSL) and American ginseng stem-leaf (AMGSL) were analyzed. The principal component analysis (PCA) model was built to discriminate Asian ginseng stem-leaf (ASGSL) from American ginseng stem-leaf (AMGSL) based on -ESI PRM MS data, and six ginsenosides (F11, Rf, R2, F1, Rb1, and Rb3) were obtained as the markers...
2018: Journal of Analytical Methods in Chemistry
Wei Liu, Ji Quan, Zeshu Hu
Food safety issues closely related to human health have always received widespread attention from the world society. As a basic food source, wheat is the fundamental support of human survival; therefore, the detection of pesticide residues in wheat is very necessary. In this work, the ultrasonic-assisted ionic liquid-dispersive liquid-liquid microextraction (DLLME) method was firstly proposed, and the extraction and analysis of three organophosphorus pesticides were carried out by combining high-performance liquid chromatography (HPLC)...
2018: Journal of Analytical Methods in Chemistry
Iñaki Elorduy, Nieves Durana, José Antonio García, María Carmen Gómez, Lucio Alonso
Thermal desorption (TD) coupled with gas chromatography/mass spectrometry (TD-GC/MS) is a simple alternative that overcomes the main drawbacks of the solvent extraction-based method: long extraction times, high sample manipulation, and large amounts of solvent waste. This work describes the optimization of TD-GC/MS for the measurement of airborne polycyclic aromatic hydrocarbons (PAHs) in particulate phase. The performance of the method was tested by Standard Reference Material (SRM) 1649b urban dust and compared with the conventional method (Soxhlet extraction-GC/MS), showing a better recovery (mean of 97%), precision (mean of 12%), and accuracy (±25%) for the determination of 14 EPA PAHs...
2018: Journal of Analytical Methods in Chemistry
Liusheng Huang, Janus Anders Juul Haagensen, Davide Verotta, Vincent Cheah, Alfred M Spormann, Francesca Aweeka, Katherine Yang
It is well known that ion-pairing reagents cause ion suppression in LC-MS/MS methods. Here, we report that trichloroacetic acid increases the MS signal of tobramycin. To support studies of an in vitro pharmacokinetic/pharmacodynamic simulator for bacterial biofilms, an LC-MS/MS method for determination of tobramycin in M9 media was developed. Aliquots of 25  μ L M9 media samples were mixed with the internal standard (IS) tobramycin-d5 (5  µ g/mL, 25  µ L) and 200  µ L 2.5% trichloroacetic acid. The mixture (5  µ L) was directly injected onto a PFP column (2...
2018: Journal of Analytical Methods in Chemistry
Ji Hyun Jeong, Seon Yu Lee, Bo Na Kim, Guk Yeo Lee, Seong Ho Ham
So-Cheong-Ryong-Tang, which is a standardized Korean medicine of the National Health Insurance, is a traditional prescription for the treatment of allergic rhinitis, bronchitis, and bronchial asthma. Simultaneous analysis and development of SCRT is essential for its stability, efficacy, and risk management. In this study, a simple, reliable, and accurate method using ultrahigh-performance liquid chromatography (UPLC) fingerprinting with a diode array detector (DAD) was developed for the simultaneous analysis...
2018: Journal of Analytical Methods in Chemistry
Dorcas Osei-Safo, Ibrahim Chikowe, Jerry Joe Ebow Kingsley Harrison, Daniel Konadu Yeboah, Ivan Addae-Mensah
A recently developed semiquantitative thin-layer chromatographic (SQ-TLC) assay has been employed in postmarketing surveillance of antimalarial medicines used in Malawi prior to HPLC assay. Both methods gave analogous results in a significant majority of the samples, with a good correlation ( r  = 0.9012) for the active pharmaceutical ingredients of the dosage forms assayed. Artemether-containing medicines had the highest percentage (92.67%) of samples with comparable results for both assays. The lowest percentage (66...
2018: Journal of Analytical Methods in Chemistry
Roberta G Machicote, Marcela A Castillo, Maria E Pacheco, Liliana Bruzzone
A flow-through optosensing system for oxazepam recognition with fluorescence detection was performed by means of a molecular imprinted polymer based on its acid hydrolysis product, 2-amino-5-chlorobenzophenone. The synthesis was conducted via a noncovalent imprinting methodology, using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent. Hydrolysis (types and concentration of acids), polymer retention capacity, binding properties, and elution (selectivity and reversibility) conditions were optimized...
2018: Journal of Analytical Methods in Chemistry
Li-Hua Chen, Yao Wu, Yong-Mei Guan, Chen Jin, Wei-Feng Zhu, Ming Yang
Fermented Cordyceps sinensis , the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS)...
2018: Journal of Analytical Methods in Chemistry
Yujie Deng, Yudong Fu, Shumin Xu, Ping Wang, Nailong Yang, Chengqian Li, Qing Yu
Limonin (LIM), a furan-containing limonoid, is one of the most abundant components of Dictamnus dasycarpus Turcz. Recent studies demonstrated that LIM has great potential for inhibiting the activity of drug-metabolizing enzymes. However, the mechanisms of LIM-induced enzyme inactivation processes remain unexplored. The main objective of this study was to identify the reactive metabolites of LIM using liquid chromatography-mass spectrometry. Three nucleophiles, glutathione (GSH), N -acetyl cysteine (NAC), and N -acetyl lysine (NAL), were used to trap the reactive metabolites of LIM in in vitro and in vivo models...
2018: Journal of Analytical Methods in Chemistry
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