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Journal of Analytical Methods in Chemistry

Andréa Claudia Oliveira Silva, Luis Carlos Ferreira de Oliveira, Angladis Vieira Delfino, Mario Roberto Meneghetti, Fabiane Caxico de Abreu
The use of nanomaterials, such as nanoparticles and nanotubes, for electrochemical detection of metal species has been investigated as a way of modifying electrodes by electrochemical stripping analysis. The present study develops a new methodology based on a comparative study of nanoparticles and nanotubes with differential pulse anodic stripping voltammetry (DPASV) and examines the simultaneous determination of copper and lead. The glassy carbon electrode modified by gold nanoparticles demonstrated increased sensitivity and decreased detection limits, among other improvements in analytical performance data...
2016: Journal of Analytical Methods in Chemistry
Slavica Filipic, Milica Elek, Marija Popović, Katarina Nikolic, Danica Agbaba
Fast and simple hydrophilic interaction liquid chromatography (HILIC) method was developed and validated for the analysis of moxonidine and its four impurities (A, B, C, and D) in pharmaceutical dosage form. All experiments were performed on the Agilent Technologies 1200 high-performance liquid chromatography (HPLC) system using Zorbax RX-SIL, 250 mm × 4.6 mm, 5 μm column as stationary phase (T = 25°C, F = 1 mL/min, and λ = 255 nm), and mixture of acetonitrile and 40 mM ammonium formate buffer (pH 2...
2016: Journal of Analytical Methods in Chemistry
Giancarlo Biancotto, Roberto Stella, Federica Barrucci, Francesca Lega, Roberto Angeletti
Despite the European ban of using anabolics in food-producing animals, growth promoters might still be illegally used in the European Union. To control the food chain and guarantee consumers' health, there is a need of highly sensitive analytical methods for the identification of marker residues of such treatments. In the present study, a group of bulls (n = 16) received trenbolone acetate (200 mg) and estradiol (40 mg) by a commercial ear implant during a time range of 71 days, and a second group (n = 16) was kept for control...
2016: Journal of Analytical Methods in Chemistry
Gen-Hua Zhao, Cui-Ping Yan, Zi-Sheng Xu, Qian-Qian Gao, Zhi-Peng Chen, Wei-Dong Li
Psoralen and isopsoralen are two isomers and main effective components within Psoralea corylifolia. In order to investigate the salt-processing effect on tissue distribution characters of psoralen and isopsoralen, a sensitive and accurate ultrahigh pressure liquid chromatographic tandem mass spectrometric (UHPLC-MS/MS) method has been developed and validated for simultaneous determination of the 2 components in rats' tissues after administration of the extracts that came from either crude or salt-processed Psoralea corylifolia L...
2016: Journal of Analytical Methods in Chemistry
Anamaria Hosu, Veronica Floare-Avram, Dana Alina Magdas, Ioana Feher, Mihai Inceu, Claudia Cimpoiu
The wine is one of the most consumed drinks over the world, being subjected to falsification or adulteration regarding the variety, vintage, and geographical region. In this study, the influence of different characteristics of wines (type, production year, and origin) on the total phenolic content, total flavonoids content, antioxidant activity, total sugars content, pH, and (18)O/(16)O isotopic ratio was investigated. The differentiation of selected wines on the basis of tested parameters was investigated using chemometric techniques, such as analysis of variance, cluster analysis, and principal component analysis...
2016: Journal of Analytical Methods in Chemistry
Jing Wang, Xiaoming Pan, Xingguo Liang
High resolution melting (HRM), with a high sensitivity to distinguish the nucleic acid species with small variations, has been widely applied in the mutation scanning, methylation analysis, and genotyping. For the aim of extending HRM for the evaluation of thermal stability of nucleic acid secondary structures on sequence dependence, we investigated effects of the dye of EvaGreen, metal ions, and impurities (such as dNTPs) on melting temperature (Tm ) measurement by HRM. The accuracy of HRM was assessed as compared with UV melting method, and little difference between the two methods was found when the DNA Tm was higher than 40°C...
2016: Journal of Analytical Methods in Chemistry
Greyce Kelly Steinhorst Alcantara, Leandro Augusto Calixto, Luiz Alberto Beraldo de Moraes, Regina Helena Costa Queiroz, Anderson Rodrigo Moraes de Oliveira, Cristiane Masetto de Gaitani
Levetiracetam (LEV) is an antiepileptic drug that is clinically effective in generalized and partial epilepsy syndromes. The use of this drug has been increasing in clinical practice and intra- or -interindividual variability has been exhibited for special population. For this reason, bioanalytical methods are required for drug monitoring in biological matrices. So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma...
2016: Journal of Analytical Methods in Chemistry
Hairui Ren, Zhaoyang Chen, Xin Zhang, Yongmei Zhao, Zheng Wang, Zhenglong Wu, Haijun Xu
Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC). The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration. The linear relationship between the integrated intensity of peak centered at ~2920 cm(-1) in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0...
2016: Journal of Analytical Methods in Chemistry
Chun Wu
Biotinylation of deoxyguanosine at an abasic site in double-stranded oligodeoxynucleotides was studied. The biotinylation of deoxyguanosine is achieved by copper-catalyzed click reaction after the conjugation of the oligodeoxynucleotide with 2-oxohex-5-ynal. The biotinylation enables visualization of the biotinylated oligodeoxynucleotides by chemiluminescence on a nylon membrane. In order to investigate the biotinylated site, the biotinylated oligodeoxynucleotides were amplified by the DNA polymerase chain reaction...
2016: Journal of Analytical Methods in Chemistry
Jing Li, Byung Kun Kim, Kang-Kyun Wang, Ji-Eun Im, Han Nim Choi, Dong-Hwan Kim, Seong In Cho, Won-Yong Lee, Yong-Rok Kim
This study demonstrates the application feasibility of electrochemical impedance spectroscopy (EIS) in measuring estrogen (17β-estradiol) in gas phase. The present biosensor gives a linear response (R(2) = 0.999) for 17β-estradiol vapor concentration from 3.7 ng/L to 3.7 × 10(-4) ng/L with a limit of detection (3.7 × 10(-4) ng/L). The results show that the fabricated biosensor demonstrates better detection limit of 17β-estradiol in gas phase than the previous report with GC-MS method. This estrogen biosensor has many potential applications for on-site detection of a variety of endocrine disrupting compounds (EDCs) in the gas phase...
2016: Journal of Analytical Methods in Chemistry
Babalwa Tembeni, Opeoluwa O Oyedeji, Ikechukwu P Ejidike, Adebola O Oyedeji
FAAS was used for the analysis of trace metals in fresh and dry plant parts of Cymbopogon validus and Hyparrhenia hirta species with the aim of determining the trace metals concentrations in selected traditional plants consumed in Eastern Cape, South Africa. The trace metal concentration (mg/kg) in the samples of dry Cymbopogon validus leaves (DCVL) showed Cu of 12.40 ± 1.000; Zn of 2.42 ± 0.401; Fe of 2.50 ± 0.410; Mn of 1.31 ± 0.210; Pb of 3.36 ± 0.401 mg/kg, while the samples of fresh Hyparrhenia hirta flowers (FHHF) gave Cu of 9...
2016: Journal of Analytical Methods in Chemistry
Helvécio C Menezes, Breno P Paulo, Maria José N Paiva, Zenilda L Cardeal
This paper describes a simple and quick method for sampling and also for carrying out the preconcentration of pesticides in environmental water matrices using two-phased hollow fiber liquid phase microextraction (HF-LPME). Factors such as extraction mode, time, solvents, agitation, and salt addition were investigated in order to validate the LPME method. The following conditions were selected: 6 cm of polypropylene hollow fiber, ethyl octanoate as an acceptor phase, and extraction during 30 min under stirring at 200 rpm...
2016: Journal of Analytical Methods in Chemistry
Lingzhi Zhao, Liu Zhao, Yanqing Miao, Chunye Liu, Chenxiao Zhang
The detection of pyrophosphatase (PPase) activity is of great significance in diagnosing diseases and understanding the function of PPase-related biological events. This study constructed a turn off-on-off fluorescent system for PPase activity assay based on PPase-regulated competitive coordination of Cu(2+) between a water-soluble fluorescent probe 6,7-dihydroxycoumarin (DHC) and pyrophosphate (PPi). The probe DHC can coordinate with Cu(2+) and consequently display on-off type fluorescence response. Furthermore, the in situ formed nonfluorescent Cu(2+)-DHC complex can act as an effective off-on type fluorescent probe for sensing PPi due to the higher coordination reactivity between Cu(2+) and PPi than that between Cu(2+) and DHC...
2016: Journal of Analytical Methods in Chemistry
Lidiane Raquel Verola Mataveli, Márcia Liane Buzzo, Luciana Juncioni de Arauz, Maria de Fátima Henriques Carvalho, Edna Emy Kumagai Arakaki, Richard Matsuzaki, Paulo Tiglea
This study is aimed at investigating a suitable method for rice sample preparation as well as validating and applying the method for monitoring the concentration of total arsenic, cadmium, and lead in rice by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Various rice sample preparation procedures were evaluated. The analytical method was validated by measuring several parameters including limit of detection (LOD), limit of quantification (LOQ), linearity, relative bias, and repeatability. Regarding the sample preparation, recoveries of spiked samples were within the acceptable range from 89...
2016: Journal of Analytical Methods in Chemistry
Carlos Cardoso-Palacios, Ingela Lanekoff
Counterfeit pharmaceutical drugs imply an increasing threat to the global public health. It is necessary to have systems to control the products that reach the market and to detect falsified medicines. In this work, molecules in several pharmaceutical tablets were directly analyzed using nanospray desorption electrospray ionization mass spectrometry (nano-DESI MS). Nano-DESI is an ambient surface sampling technique which enables sampling of molecules directly from the surface of the tablets without any sample pretreatment...
2016: Journal of Analytical Methods in Chemistry
Chalder Nogueira Nunes, Lucas Ely Pauluk, Maria Lurdes Felsner, Vanessa Egéa Dos Anjos, Sueli Pércio Quináia
17α-Ethinyl estradiol (EE2), which is used worldwide in the treatment of some cancers and as a contraceptive, is often found in aquatic systems and is considered a pharmaceutically active compound (PhACs) in the environment. Current methods for the determination of this compound, such as chromatography, are expensive and lengthy and require large amounts of toxic organic solvents. In this work, a voltammetric procedure is developed and validated as a screening tool for detecting EE2 in water samples without prior extraction, clean-up, or derivatization steps...
2016: Journal of Analytical Methods in Chemistry
Thamani T Gondo, Veronica C Obuseng, Lesego C Mmualefe, Harriet Okatch
HS-SPME was optimised using blank plant sample for analysis of organochlorine pesticides (OCPs) of varying polarities in selected medicinal plants obtained from northern part of Botswana, where OCPs such as DDT and endosulfan have been historically applied to control disease carrying vectors (mosquitos and tsetse fly). The optimised SPME parameters were used to isolate analytes from root samples of five medicinal plants obtained from Maun and Kasane, Botswana. The final analytes determination was done with a gas chromatograph equipped with GC-ECD and analyte was confirmed using electron ionisation mass spectrometer (GC-MS)...
2016: Journal of Analytical Methods in Chemistry
Xiaodong Wei, Hongling Shen, Lijun Wang, Qingyan Meng, Wenjie Liu
A comprehensive two-dimensional (2D) reverse phase (RP) liquid chromatography (LC) method is developed for alkaloid analysis. This offline comprehensive 2D method is developed using different pH values. With a pH value of 10.5, most alkaloids appear in the form of neutral molecules possessing high retention factors based on their polarity, while the alkaloid polarity order is changed when the pH value decreased to 3.0. The performance of pH modulated 2D LC is demonstrated with 8 alkaloid standards which resulted in orthogonal separation...
2016: Journal of Analytical Methods in Chemistry
Yang Liu, Zhaoxia Liu, Huaxin Yang, Lan He
Quantitative nuclear magnetic resonance (qNMR) is a powerful tool in measuring drug content because of its high speed, sensitivity, and precision. Most of the reports were based on proton qNMR ((1)H qNMR) and only a few fluorine qNMR ((19)F qNMR) were reported. No research has been conducted to directly compare the advantage and disadvantage between these two methods. In the present study, both (19)F and (1)H qNMR were performed to characterize the content of atorvastatin calcium with the same internal standard...
2016: Journal of Analytical Methods in Chemistry
Alireza Feizbakhsh, Amir Hossein Mohsen Sarrafi, Shokooh Ehteshami
Polythiophene/chitosan magnetic nanocomposite as an adsorbent of magnetic solid phase extraction was proposed for the isolation of fluoxetine in aqueous and biological samples prior to fluorescence detection at 246 nm. The synthesized nanoparticles, chitosan and polythiophene magnetic nanocomposite, were characterized by scanning electron microscopy, FT-IR, TGA, and EDAX. The separation of the target analyte from the aqueous solution containing the fluoxetine and polythiophene/chitosan magnetic nanocomposite was simply achieved by applying external magnetic field...
2016: Journal of Analytical Methods in Chemistry
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