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Annals of Laboratory Medicine

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https://www.readbyqxmd.com/read/28224782/erratum-detection-of-first-line-anti-tuberculosis-drug-resistance-mutations-by-allele-specific-primer-extension-on-a-microsphere-based-platform
#1
(no author information available yet)
This corrects the article on p. 487 in vol. 35, PMID: 26206684.
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224781/a-puzzling-switch-in-blood-type-following-blood-transfusion
#2
LETTER
Luiza Akria, Judith Chezar, Simona Zisman-Rozen, Eyal J Scheinman, Zeev Zonis, Yoav Hoffmann, Tzipora Falik-Zaccai, Limor Kalfon, Michael Weiss, Andrei Braester, Celia Suriu, Masad Barhoum, Amir Kuperman, Ety Shaoul
No abstract text is available yet for this article.
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224780/two-cases-of-antibody-mediated-rejection-following-kidney-transplantation-due-to-hla-dqb1-allele-specific-and-dq-alpha-protein-specific-hla-antibodies
#3
LETTER
Seung Hwan Song, Borae G Park, Beom Seok Kim, Yu Seun Kim, Hyon Suk Kim
No abstract text is available yet for this article.
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224779/vancomycin-resistance-due-to-vana-gene-expression-in-an-aerococcus-viridans-isolate-first-case-in-korea
#4
LETTER
Kwangjin Ahn, Gyu Yel Hwang, Young Uh, Kap Jun Yoon, Shinyoung Hyun
No abstract text is available yet for this article.
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224778/nup98-rearrangement-in-acute-myelomonocytic-leukemia-with-t-11-19-p15-p12-the-first-case-report-worldwide
#5
LETTER
Hyeon Ho Lim, Gyu Dae An, Kwang Sook Woo, Kyeong Hee Kim, Jeong Man Kim, Sung Hyun Kim, Jin Yeong Han
No abstract text is available yet for this article.
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224777/the-first-case-of-therapy-related-myelomastocytic-leukemia-based-on-the-who-2008-classification-and-the-recently-proposed-diagnostic-criteria
#6
LETTER
Won Kyu Choi, Young Uk Cho, Eunkyoung You, Seongsoo Jang, Eul Ju Seo, Chan Jeoung Park
No abstract text is available yet for this article.
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224776/clinical-usefulness-of-monitoring-cytomegalovirus-specific-immunity-by-quantiferon-cmv-in-pediatric-allogeneic-hematopoietic-stem-cell-transplantation-recipients
#7
Sae Mi Lee, Yae Jean Kim, Keon Hee Yoo, Ki Woong Sung, Hong Hoe Koo, Eun Suk Kang
Cytomegalovirus (CMV) is a well-established cause of morbidity and mortality in pediatric recipients of allogeneic hematopoietic stem cell transplantation (allo-HSCT). CD8⁺ T-cells are important for controlling CMV infection. We conducted a prospective pilot study to investigate the clinical utility of measuring the CMV-specific T-cell immune response using the QuantiFERON-CMV assay (QF-CMV) in pediatric allo-HSCT recipients. Overall, 16 of 25 (64%) patients developed CMV infection. QF-CMV was evaluated in these 16 patients during the early and late phases of the first CMV infection post allo-HSCT...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224775/laboratory-identification-of-leptotrichia-species-isolated-from-bacteremia-patients-at-a-single-institution
#8
Eun Hye Cho, Kyung Sun Park, Mina Yang, Dong Joon Song, Hee Jae Huh, Chang Seok Ki, Nam Yong Lee
We describe the laboratory identification of Leptotrichia species from clinical isolates collected over a six-year period. Five isolates from blood cultures were identified as Leptotrichia species. Gram stain showed large, fusiform, gram-negative or -variable bacilli. Identification based on biochemical testing was unsuccessful; however, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry proved to be a useful tool for identifying Leptotrichia species to the genus level. Species level identification was successfully achieved by using 16S ribosomal RNA gene sequencing...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224774/comparison-of-luminex-nxtag-respiratory-pathogen-panel-and-xtag-respiratory-viral-panel-fast-version-2-for-the-detection-of-respiratory-viruses
#9
COMPARATIVE STUDY
Chun Kiat Lee, Hong Kai Lee, Christopher Wei Siong Ng, Lily Chiu, Julian Wei Tze Tang, Tze Ping Loh, Evelyn Siew Chuan Koay
Owing to advancements in molecular diagnostics, recent years have seen an increasing number of laboratories adopting respiratory viral panels to detect respiratory pathogens. In December 2015, the NxTAG respiratory pathogen panel (NxTAG RPP) was approved by the United States Food and Drug Administration. We compared the clinical performance of this new assay with that of the xTAG respiratory viral panel (xTAG RVP) FAST v2 using 142 clinical samples and 12 external quality assessment samples. Discordant results were resolved by using a laboratory-developed respiratory viral panel...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224773/novel-slc37a4-mutations-in-korean-patients-with-glycogen-storage-disease-ib
#10
Rihwa Choi, Hyung Doo Park, Jung Min Ko, Jeongho Lee, Dong Hwan Lee, Suk Jin Hong, Chang Seok Ki, Soo Youn Lee, Jong Won Kim, Junghan Song, Yon Ho Choe
BACKGROUND: Molecular techniques are fundamental for establishing an accurate diagnosis and therapeutic approach of glycogen storage diseases (GSDs). We aimed to evaluate SLC37A4 mutation spectrum in Korean GSD Ib patients. METHODS: Nine Korean patients from eight unrelated families with GSD Ib were included. SLC37A4 mutations were detected in all patients with direct sequencing using a PCR method and/or whole-exome sequencing. A comprehensive review of previously reported SLC37A4 mutations was also conducted...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224772/effects-of-neutralization-by-soluble-abh-antigens-produced-by-transplanted-kidneys-from-abo-incompatible-secretor-donors
#11
Jieun Kim, Sinyoung Kim, In Sik Hwang, Jong Rak Choi, Jae Geun Lee, Yu Seun Kim, Myoung Soo Kim, Hyun Ok Kim
BACKGROUND: Grafts survive despite blood group antigens on the transplant being continuously exposed to antibodies in the blood of recipients in ABO-incompatible kidney transplantation (ABOi KT), owing to the mechanism of accommodation. We analyzed the immunodynamics of soluble ABH antigens in allografts from secretor donors and the influence of such immunodynamics on accommodation and subsequent graft survival in ABOi KT. METHODS: The genotype of a known human β-galactoside α-1,2-fucosyltransferase gene (FUT2), which determines soluble ABH antigen secretor status, was established in 32 donors for ABOi KT at the Severance Hospital, from June 2010 to July 2015...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224771/comparison-of-the-qiagen-artus-hbv-qs-rgq-assay-with-the-roche-cobas-ampliprep-cobas-taqman-hbv-assay-for-quantifying-viral-dna-in-sera-of-chronic-hepatitis-b-patients
#12
COMPARATIVE STUDY
Mi Soon Han, Yongjung Park, Hyunjin Nah, Hyon Suk Kim
BACKGROUND: Hepatitis B virus DNA quantification is essential for managing chronic hepatitis B (CHB). We compared the performance of artus HBV QS-RGQ (QIAGEN GmbH, Germany) and CAP/CTM v2.0 HBV assays (Roche Molecular Diagnostics, USA) in CHB patients. METHODS: A comparative evaluation between two assays was performed with 508 clinical serum samples. Precision, linearity, and the limit of detection (LOD) of QS-RGQ assay was evaluated by using the WHO standard 97/750 and clinical samples...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224770/performance-of-an-automated-fluorescence-antinuclear-antibody-image-analyzer
#13
In Young Yoo, Jong Won Oh, Hoon Suk Cha, Eun Mi Koh, Eun Suk Kang
BACKGROUND: The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing. We evaluated the performance of EUROPattern Suite (Euroimmun AG, Germany), an automated FANA image analyzer, with regard to ANA detection and pattern recognition compared with conventional manual interpretation using the fluorescence microscopic IIF assay...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224769/increasing-resistance-to-extended-spectrum-cephalosporins-fluoroquinolone-and-carbapenem-in-gram-negative-bacilli-and-the-emergence-of-carbapenem-non-susceptibility-in-klebsiella-pneumoniae-analysis-of-korean-antimicrobial-resistance-monitoring-system-karms
#14
Dokyun Kim, Ji Young Ahn, Chae Hoon Lee, Sook Jin Jang, Hyukmin Lee, Dongeun Yong, Seok Hoon Jeong, Kyungwon Lee
BACKGROUND: National surveillance of antimicrobial resistance becomes more important for the control of antimicrobial resistance and determination of treatment guidelines. We analyzed Korean Antimicrobial Resistance Monitoring System (KARMS) data collected from 2013 to 2015. METHODS: Of the KARMS participants, 16 secondary or tertiary hospitals consecutively reported antimicrobial resistance rates from 2013 to 2015. Data from duplicate isolates and institutions with fewer than 20 isolates were excluded...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224768/comparison-of-the-bruker-biotyper-and-vitek-ms-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-systems-using-a-formic-acid-extraction-method-to-identify-common-and-uncommon-yeast-isolates
#15
COMPARATIVE STUDY
Hyun Seung Lee, Jong Hee Shin, Min Ji Choi, Eun Jeong Won, Seung Jung Kee, Soo Hyun Kim, Myung Geun Shin, Soon Pal Suh
BACKGROUND: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) allows rapid and accurate identification of clinical yeast isolates. In-tube formic acid/acetonitrile (FA/ACN) extraction is recommended prior to the analysis with MALDI Biotyper, but the direct on-plate FA extraction is simpler. We compared the Biotyper with the VITEK MS for the identification of various clinically relevant yeast species, focusing on the use of the FA extraction method...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224767/effect-of-accreditation-on-accuracy-of-diagnostic-tests-in-medical-laboratories
#16
Mi Ae Jang, Young Ahn Yoon, Junghan Song, Jeong Ho Kim, Won Ki Min, Ji Sung Lee, Yong Wha Lee, You Kyoung Lee
BACKGROUND: Medical laboratories play a central role in health care. Many laboratories are taking a more focused and stringent approach to quality system management. In Korea, laboratory standardization efforts undertaken by the Korean Laboratory Accreditation Program (KLAP) and the Korean External Quality Assessment Scheme (KEQAS) may have facilitated an improvement in laboratory performance, but there are no fundamental studies demonstrating that laboratory standardization is effective...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224766/thromboelastographic-evaluation-of-coagulation-in-patients-with-liver-disease
#17
Kyung Hwa Shin, In Suk Kim, Hyun Ji Lee, Hyung Hoi Kim, Chulhun L Chang, Young Mi Hong, Ki Tae Yoon, Mong Cho
BACKGROUND: The aims of this study were to investigate the parameters of thromboelastography (TEG) for evaluating coagulopathy and to reveal an association with disease severity and/or transfusion requirement in patients with chronic liver disease (CLD) in a clinical laboratory setting. METHODS: We enrolled two groups of adult patients with cirrhotic (N=123) and non-cirrhotic liver disease (N=52), as well as 84 healthy controls. Reaction time (R), kinetic time (K), α-angle (α), maximal amplitude (MA), and coagulation index (CI) were measured with kaolin-activated citrated blood with the TEG 5000 system (Haemonetics Corporation, USA)...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28224765/dysregulation-of-telomere-lengths-and-telomerase-activity-in-myelodysplastic-syndrome
#18
Hee Sue Park, Jungeun Choi, Cha Ja See, Jung Ah Kim, Si Nae Park, Kyongok Im, Sung Min Kim, Dong Soon Lee, Sang Mee Hwang
BACKGROUND: Telomere shortening is thought to be involved in the pathophysiology of myeloid malignancies, but telomere lengths (TL) during interphase and metaphase in hematopoietic malignancies have not been analyzed. We aimed to assess the TLs of interphase and metaphase cells of MDS and telomerase activity (TA) and to find out prognostic significances of TL and TA. METHODS: The prognostic significance of TA by quantitative PCR and TL by quantitative fluorescence in situ hybridization (QFISH) of interphase nuclei and metaphase chromosome arms of bone marrow cells from patients with MDS were evaluated...
May 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28029011/clinical-pharmacogenetic-testing-and-application-laboratory-medicine-clinical-practice-guidelines
#19
Sollip Kim, Yeo Min Yun, Hyo Jin Chae, Hyun Jung Cho, Misuk Ji, In Suk Kim, Kyung A Wee, Woochang Lee, Sang Hoon Song, Hye In Woo, Soo Youn Lee, Sail Chun
Pharmacogenetic testing for clinical applications is steadily increasing. Correct and adequate use of pharmacogenetic tests is important to reduce unnecessary medical costs and adverse patient outcomes. This document contains recommended pharmacogenetic testing guidelines for clinical application, interpretation, and result reporting through a literature review and evidence-based expert opinions for the clinical pharmacogenetic testing covered by public medical insurance in Korea. This document aims to improve the utility of pharmacogenetic testing in routine clinical settings...
March 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28029010/concomitant-aid-expression-and-bcl7a-loss-associates-with-accelerated-phase-progression-and-imatinib-resistance-in-chronic-myeloid-leukemia
#20
LETTER
Nae Yu, Saeam Shin, Jong Rak Choi, Yoonjung Kim, Kyung A Lee
No abstract text is available yet for this article.
March 2017: Annals of Laboratory Medicine
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