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Annals of Laboratory Medicine

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https://www.readbyqxmd.com/read/30430792/erratum-novel-4-bp-intronic-deletion-c-1560-3_1560-6del-in-lemd3-in-a-korean-patient-with-osteopoikilosis
#1
(no author information available yet)
This corrects the article on p. 540 in vol. 37, PMID: 28840995.
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430791/advantages-of-array-comparative-genomic-hybridization-using-buccal-swab-dna-for-detecting-pallister-killian-syndrome
#2
LETTER
Veronica Bertini, Simone Gana, Alessandro Orsini, Alice Bonuccelli, Diego Peroni, Valetto Angelo
No abstract text is available yet for this article.
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430790/the-first-case-of-congenital-prekallikrein-deficiency-in-korea-with-a-novel-pathogenic-variant-c-1198g-t
#3
LETTER
Sohee Ryu, Ja Yoon Gu, Kyung Taek Hong, Doo Hee Han, Hyun Kyung Kim
No abstract text is available yet for this article.
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430789/centromere-protein-f-like-pattern-in-a-patient-with-rheumatoid-arthritis
#4
LETTER
Kyuhwa Hur, La He Jearn, Think You Kim
No abstract text is available yet for this article.
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430788/reconstructed-three-dimensional-images-and-parameters-of-individual-erythrocytes-using-optical-diffraction-tomography-microscopy
#5
Se Eun Koo, Seongsoo Jang, YongKeun Park, Chan Jeoung Park
No abstract text is available yet for this article.
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430787/red-blood-cell-alloimmunization-in-korean-patients-with-myelodysplastic-syndrome-and-liver-cirrhosis
#6
Hyun Young Kim, Eun Jung Cho, Sejong Chun, Kyeong Hee Kim, Duck Cho
Red blood cell (RBC) alloimmunization varies across human populations and ethnic groups. We evaluated the characteristics of RBC alloimmunization and compared the risk of alloimmunization in Korean patients with myelodysplastic syndrome (MDS) and liver cirrhosis (LC), two representative diseases in which chronic transfusion is required. In total, 115 MDS patients and 202 LC patients transfused with RBCs between 2013 and 2015 were retrospectively included. Twenty patients (6.3%) were newly alloimmunized (five MDS patients, 4...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430786/simultaneous-detection-of-clostridioides-difficile-glutamate-dehydrogenase-and-toxin-a-b-comparison-of-the-c-diff-quik-chek-complete-and-ridascreen-assays
#7
In Young Yoo, Dong Joon Song, Hee Jae Huh, Nam Yong Lee
Various commercial assays have recently been developed for detecting glutamate dehydrogenase (GDH) and/or toxin A/B to diagnose Clostridioides difficile infection (CDI). We compared the performance of two assays for the simultaneous detection of C. difficile GDH and toxin A/B, using 150 stool samples: C. DIFF QUIK CHEK COMPLETE (QCC; TechLab, Blacksburg, VA, USA) and RIDASCREEN Clostridium difficile GDH (RC-GDH) and Toxin A/B (RC-Toxin A/B; R-Biopharm, Darmstadt, Germany). For GDH detection, QCC and RC-GDH showed satisfactory sensitivity (95...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430785/hemoglobin-a1c-levels-are-slightly-but-significantly-lower-in-normoglycemic-subjects-with-the-hemoglobin-e-phenotype
#8
Busadee Pratumvinit, Kanit Reesukumal, Sithikan Hanyongyuth, Sujitra Wangchaijaroenkit, Julaporn Pooliam, Gerald J Kost, Panumas Kamkang, Tze Ping Loh
Hb mutations can alter the structure, behavior, stability, or quantity of the globin chain produced. Some Hb variants shorten the erythrocyte life span, resulting in physiologically lower hemoglobin A1c (HbA1c) levels. The hemoglobin E (HbE) phenotype involves a single-nucleotide polymorphism that reduces β-globin chain synthesis. We compared the HbA1c levels of subjects with normal Hb (HbAA; N=131) and HbE (N=148) phenotypes, examining potential hematological and biochemical factors contributing to differences in HbA1c levels...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430784/estimated-glomerular-filtration-rates-show-minor-but-significant-differences-between-the-single-and-subgroup-creatinine-based-chronic-kidney-disease-epidemiology-collaboration-equations
#9
Sholhui Park, Tae Dong Jeong
The creatinine-based Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation can be calculated according to race, sex, and creatinine concentration (subgroup equation) or in the form expressed by one equation (single equation). Minor differences in the constants used in the CKD-EPI equations (subgroup vs single equations) could result in a significant difference in the estimated glomerular filtration rate (eGFR). We evaluated the impact of this difference in 79,709 Korean patients. The eGFR was calculated as an integer using the single and subgroup CKD-EPI equations...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430783/cluster-containing-more-than-20-cd3-positive-cells-in-bone-marrow-biopsy-is-a-candidate-prognostic-indicator-in-peripheral-t-cell-lymphoma-not-otherwise-specified
#10
Hyoeun Shim, Seongsoo Jang, Jooryung Huh, Dok Hyun Yoon, Cheolwon Suh, Chan Jeoung Park
Assessment of bone marrow (BM) involvement in peripheral T-cell lymphoma, not otherwise specified (PTCL) is straightforward in cases of extensive involvement but difficult in cases of minimal to partial involvement. We evaluated the usefulness of CD3 as an immunohistochemical marker for assessing BM involvement in PTCL patients. BM biopsies of 92 PTCL patients were immunohistochemically stained for CD3, CD4, CD8, CD20, and CD56, and evaluated by two hematopathologists. CD3 positivity was graded according to the proportion of CD3-positive cells and the number of CD3-positive cells in a cluster...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430782/antimicrobial-susceptibility-patterns-of-anaerobic-bacterial-clinical-isolates-from-2014-to-2016-including-recently-named-or-renamed-species
#11
Jung Hyun Byun, Myungsook Kim, Yangsoon Lee, Kyungwon Lee, Yunsop Chong
BACKGROUND: Anaerobic bacterial resistance trends may vary across regions or institutions. Regional susceptibility patterns are pivotal in the empirical treatment of anaerobic infections. We determined the antimicrobial resistance patterns of clinically important anaerobic bacteria, including recently named or renamed anaerobes. METHODS: A total of 521 non-duplicated clinical isolates of anaerobic bacteria were collected from a tertiary-care hospital in Korea between 2014 and 2016...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430781/an-excel-macro-for-determining-allelic-and-sequence-types-of-bacterial-clones-in-multilocus-sequence-typing
#12
Yu Jin Park, Min Hyuk Choi, Dokyun Kim, Kwangjun Lee, Hyun Ok Kim, Seok Hoon Jeong
BACKGROUND: Multilocus sequence typing (MLST) was designed to overcome the low discriminatory power and poor reproducibility of previous molecular typing schemes, and it is useful for inter-laboratory, inter-regional, and inter-national comparison of pathogenic clones. MLST includes labor-intensive sequencing processes and meticulous allelic/sequence type (ST) determination processes, often prone to error. We developed a free automated MLST determination program (MLST typer) based on the Visual Basic for Applications macro, which runs on Microsoft Excel...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430780/comparative-evaluation-between-the-realstar-pneumocystis-jirovecii-pcr-kit-and-the-amplisens-pneumocystis-jirovecii-carinii-frt-pcr-kit-for-detecting-p-jirovecii-in-non-hiv-immunocompromised-patients
#13
Hee Jae Huh, Kyoung Ree Lim, Chang Seok Ki, Kyungmin Huh, Hyang Jin Shim, Dong Joon Song, Yae Jean Kim, Doo Ryeon Chung, Nam Yong Lee
BACKGROUND: Real-time PCR is more sensitive than microscopic examination for detecting Pneumocystis jirovecii . We compared the performance of two assays for detecting P. jirovecii DNA: the RealStar Pneumocystis jirovecii PCR Kit 1.0 CE (Altona Diagnostics, Hamburg, Germany) and the AmpliSens Pneumocystis jirovecii ( carinii )-FRT PCR kit (InterLabService Ltd., Moscow, Russia). METHODS: We used 159 samples from the lower respiratory tract (112 bronchoalveolar lavage [BAL] fluid, 37 sputum, and 10 endotracheal aspirate [ETA] samples) of non-HIV immunocompromised patients...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430779/identification-and-characterization-of-ndm-1-producing-hypervirulent-hypermucoviscous-klebsiella-pneumoniae-in-china
#14
Zhou Liu, Yi Gu, Xin Li, Yanyan Liu, Ying Ye, Shihe Guan, Jiabin Li
BACKGROUND: Carbapenem-resistant hypervirulent (hypermucoviscous) Klebsiella pneumoniae (CR-HMKP) poses a significant public health challenge. We investigated its epidemiology and molecular characteristics in a tertiary care hospital in eastern China. METHODS: CR-HMKP were identified among 106 non-duplicated carbapenem-resistant K. pneumoniae isolates (from June 2013 to September 2017) using the string test. The pulsotype (PT) and sequence type (ST) of CR-HMKP isolates were determined using pulsed-field gel electrophoresis and multilocus sequence typing...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430778/impact-of-community-onset-methicillin-resistant-staphylococcus-aureus-on-staphylococcus-aureus-bacteremia-in-a-central-korea-veterans-health-service-hospital
#15
Eunsin Bae, Choon Kwan Kim, Jung Hyun Jang, Heungsup Sung, YounMi Choi, Mi Na Kim
BACKGROUND: No study has examined the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in Korean veterans' hospitals. We investigated the microbiological and clinical epidemiology of S. aureus bacteremia at the central Veterans Health Services (VHS) hospital in Korea. METHODS: Patients with S. aureus bacteremia were consecutively enrolled from February to August 2015. Bacteremia was classified as hospital-acquired (HA), community-onset healthcare-associated (COHA), or community-acquired (CA)...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430777/comparison-of-high-sensitivity-and-conventional-flow-cytometry-for-diagnosing-overt-paroxysmal-nocturnal-hemoglobinuria-and-detecting-minor-paroxysmal-nocturnal-hemoglobinuria-clones
#16
Sang Hyuk Park, Joseph Jeong, Seon Ho Lee, Dong Won Yoo, Yunsuk Choi, Jae Cheol Jo, Ji Hun Lim
BACKGROUND: High sensitivity flow cytometry (HS-FCM) was recently developed for diagnosing paroxysmal nocturnal hemoglobinuria (PNH). We compared its performance with conventional flow cytometry (C-FCM) for diagnosing overt PNH and detecting minor (0.1-1%) PNH clones in aplastic anemia (AA)/low-grade myelodysplastic syndrome (MDS) patients. METHODS: C-FCM and HS-FCM were performed simultaneously on 41 samples from healthy controls and 23 peripheral blood samples from 15 AA/low-grade MDS and eight PNH patients, using a Navios flow cytometer (Beckman Coulter, Miami, FL, USA)...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430776/development-of-a-novel-flow-cytometry-based-system-for-white-blood-cell-differential-counts-10-color-leukodiff
#17
Dongjin Park, Jiyoung Chang, Jimin Kahng, Hunhee Park, Irene Jo, Yonggoo Kim, Kyungja Han
BACKGROUND: Flow cytometry (FCM) is commonly used to identify many cell populations. We developed a white blood cell (WBC) differential counting system for detecting abnormal cells using FCM incorporating 10 colors and 11 antibodies in a single tube, called "10-color LeukoDiff," and evaluated its performance. METHODS: Ninety-one EDTA-anti-coagulated peripheral blood samples from 76 patients were analyzed using 10-color LeukoDiff. We compared 10 color LeukoDiff results with the results of manual differential count (manual diff)...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430775/increased-prevalence-of-thalassemia-in-young-people-in-korea-impact-of-increasing-immigration
#18
Hyun Ji Lee, Kyung Hwa Shin, Hyung Hoi Kim, Eu Jeen Yang, Kyung Hee Park, Min Ju Kim, Jeong Ran Kwon, Young Sil Choi, Jun Nyun Kim, Myung Geun Shin, Yong Gon Cho, Sun Jun Kim, Kyeong Hee Kim, Seri Jeong, Seom Gim Kong, Yu Jin Jung, Nayoung Lee, Man Jin Kim, Moon Woo Seong
BACKGROUND: Thalassemia is highly prevalent in Southeast Asia but is rare in Korea; however, Southeast Asian immigrant population is recently rising in Korea. We investigated the prevalence of thalassemia in Korea in the context of increasing immigration. METHODS: This prospective, observational, multicenter study was conducted between September 2015 and August 2017. A total of 669 subjects <30 years living in Korea were grouped into the multiethnic (N=314) and Korean (N=355) groups...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430774/t-cell-receptor-rearrangements-determined-using-fragment-analysis-in-patients-with-t-acute-lymphoblastic-leukemia
#19
Hyerim Kim, In Suk Kim, Chulhun L Chang, Sun Young Kong, Young Tak Lim, Seom Gim Kong, Eun Hae Cho, Eun Yup Lee, Ho Jin Shin, Hyeon Jin Park, Hyeon Seok Eom, Hyewon Lee
BACKGROUND: Chromosomal abnormalities and common genetic rearrangements related to T-acute lymphoblastic leukemia (T-ALL) are not clear. We investigated T-cell receptor ( TCR ) rearrangement in Korean T-ALL patients by fragment analysis, examining frequency, association between clinicopathologic characteristics and TCR clonality, and feasibility for detecting minimal residual disease (MRD). METHODS: In 51 Korean patients diagnosed as having T-ALL, TCR rearrangement was analyzed using the IdentiClone TCR gene clonality assay (InVivoScribe Technologies, San Diego, CA, USA) from archived bone marrow specimens...
March 2019: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/30430773/reducing-diagnostic-errors-worldwide-through-diagnostic-management-teams
#20
REVIEW
Roberto Verna, Adriana Berumen Velazquez, Michael Laposata
A major challenge facing most countries is the growing cost of healthcare. Laboratory testing costs constitute approximately 3% of all clinical costs, while waste of funds due to inappropriate admissions to clinical departments is reported to be as high as 15%. A frequently used approach to save money in healthcare is random reduction of laboratory budgets, focusing on decreasing the number of unnecessary laboratory tests. The World Health Assembly has approached this problem by publishing a list of essential in vitro diagnostic tests, to achieve a global rationalization of the problem...
March 2019: Annals of Laboratory Medicine
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