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European Journal of Microbiology & Immunology

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https://www.readbyqxmd.com/read/28690882/photoinactivation-of-legionella-rubrilucens-by-visible-light
#1
J Schmid, K Hoenes, M Rath, P Vatter, B Spellerberg, M Hessling
In this study, the photoinactivation of Legionella by visible light is investigated. The success of this approach would offer new prospects for technical water disinfection and maybe even for therapeutic measures in cases of Legionella infections. Therefore, Legionella rubrilucens was dispensed on buffered charcoal yeast extract medium agar plates and illuminated with different doses of violet light generated by 405 nm light-emitting diodes (LEDs). A strong photoinactivation effect was observed. A dose of 125 J/ cm(2) reduced the bacterial concentration by more than 5 orders of magnitude compared to Legionella on unirradiated agar plates...
June 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28690881/small-intestinal-pro-inflammatory-immune-responses-following-campylobacter-jejuni-infection-of-secondary-abiotic-il-10-mice-lacking-nucleotide-oligomerization-domain-2
#2
Markus M Heimesaat, Ursula Grundmann, Marie E Alutis, André Fischer, Stefan Bereswill
Host immune responses are crucial for combating enteropathogenic infections including Campylobacter jejuni. Within 1 week following peroral C. jejuni infection, secondary abiotic IL-10(-/-) mice develop severe immunopathological sequelae affecting the colon (ulcerative enterocolitis). In the present study, we addressed whether pathogen-induced pro-inflammatory immune responses could also be observed in the small intestines dependent on the innate receptor nucleotide-oligomerization-domain-protein 2 (Nod2). Within 7 days following peroral infection, C...
June 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28690880/the-prevalence-of-ctx-m-15-extended-spectrum-%C3%AE-lactamases-among-salmonella-spp-and-shigella-spp-isolated-from-three-iranian-hospitals
#3
Abed Zahedi Bialvaei, Tala Pourlak, Mina Aghamali, Mohammad Asgharzadeh, Pourya Gholizadeh, Hossein Samadi Kafil
Bacterial antimicrobial resistance mediated by the production of extended-spectrum β-lactamases (ESBLs) is considered a major threat for treatment of Salmonella and Shigella infections. This study aimed to investigate antibiotic resistance patterns of Salmonella and Shigella spp. and presence of CTX-M from three teaching hospitals in Iran. In the present study, 58 clinical Shigella and 91 Salmonella isolates were recovered between 2009 and 2013 from 3 teaching hospitals in Iran. After culture and antimicrobial susceptibility testing, ESBL-positive isolates were subjected to further investigations...
June 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28690879/epidemiological-characteristics-and-clinical-outcome-of-hiv-related-tuberculosis-in-a-population-of-tb-patients-in-south-western-nigeria
#4
Olugbenga A Olowe, Olufunmilola B Makanjuola, Adeniyi S Adekanmi, Olusola J Adefioye, Rita A Olowe
Tuberculosis (TB) is the second leading cause of death from infectious disease globally with its impact more dramatic in resource limited settings. Individuals with human immunodeficiency virus (HIV) infection who also develop tuberculosis represent a significant challenge to TB control. This study was carried out to determine the prevalence of TB-HIV coinfection and pattern of infection among TB patients. We also compared treatment outcome among coinfected patients with those not coinfected. A six-year retrospective review of records of patients managed at the Tuberculosis Treatment Center of the LAUTECH Teaching Hospital, South-Western Nigeria from January 2009 to December 2014 was carried out...
June 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28690878/the-guanine-nucleotide-exchange-factor-caldag-gefi-fine-tunes-functional-properties-of-regulatory-t-cells
#5
Jana Niemz, Stefanie Kliche, Marina C Pils, Eliot Morrison, Annika Manns, Christian Freund, Jill R Crittenden, Ann M Graybiel, Melanie Galla, Lothar Jänsch, Jochen Huehn
Using quantitative phosphopeptide sequencing of unstimulated versus stimulated primary murine Foxp3(+) regulatory and Foxp3(-) conventional T cells (Tregs and Tconv, respectively), we detected a novel and differentially regulated tyrosine phosphorylation site within the C1 domain of the guanine-nucleotide exchange factor CalDAG GEFI. We hypothesized that the Treg-specific and activation-dependent reduced phosphorylation at Y523 allows binding of CalDAG GEFI to diacylglycerol, thereby impacting the formation of a Treg-specific immunological synapse...
June 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28690877/colonization-with-multidrug-resistant-bacteria-on-the-efficiency-of-local-decolonization-procedures
#6
Julia Münch, Ralf Matthias Hagen, Martin Müller, Viktor Kellert, Dorothea Franziska Wiemer, Rebecca Hinz, Norbert Georg Schwarz, Hagen Frickmann
The effectiveness of a disinfectant-based decolonization strategy for multidrug-resistant bacteria like extended spectrum β-lactamase (ESBL)-positive Gram-negative bacteria with or without additional fluoroquinolon and carbapenem resistance as well as vancomycin-resistant enterococci and methicillin-resistant Staphylococcus aureus was assessed. Between 2011 and 2015, 25 patients from Libya, Syria, and the Ukraine with war traumata were treated at the Bundeswehr hospital Hamburg. The patients were heavily colonized and infected with multidrug-resistant bacteria, altogether comprising 371 distinct combinations of pathogens and isolation sites...
June 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28386474/finding-novel-antibiotic-substances-from-medicinal-plants-antimicrobial-properties-of-nigella-sativa-directed-against-multidrug-resistant-bacteria
#7
Seher Nancy Bakal, Stefan Bereswill, Markus M Heimesaat
The progressive rise in multidrug-resistant (MDR) bacterial strains poses serious problems in the treatment of infectious diseases. While the number of newly developed antimicrobial compounds has greatly fallen, the resistance of pathogens against commonly prescribed drugs is further increasing. This rise in resistance illustrates the need for developing novel therapeutic and preventive antimicrobial options. The medicinal herb Nigella sativa and its derivatives constitute promising candidates. In a comprehensive literature survey (using the PubMed data base), we searched for publications on the antimicrobial effects of N...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28386473/isolation-of-saccharomyces-cerevisiae-from-pineapple-and-orange-and-study-of-metal-s-effectiveness-on-ethanol-production
#8
Armanul Nasir, Shafkat Shamim Rahman, Md Mahboob Hossain, Naiyyum Choudhury
In view of the anticipated shortage of the traditional supplies of fossil fuels, there is a great deal of interest in the production of ethanol as an alternative biofuel in recent years. The main objective of this research work was to isolate and characterize stress tolerant, high potential ethanol producing yeast strains from various fruit peel. Two yeast isolates from pineapple (Pa) and orange (Or) have been isolated, characterized on the basis of morphological and physic-chemical characters and optimized on ethanol producing capability using sugarcane molasses as substrate...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28386472/comprehensive-survey-of-intestinal-microbiota-changes-in-offspring-of-human-microbiota-associated-mice
#9
Eliane von Klitzing, Fulya Öz, Ira Ekmekciu, Ulrike Escher, Stefan Bereswill, Markus M Heimesaat
Secondary abiotic mice generated by broad-spectrum antibiotic treatment provide a valuable tool for association studies with microbiota derived from different vertebrate hosts. We here generated human microbiota-associated (hma) mice by human fecal microbiota transplantation of secondary abiotic mice and performed a comprehensive survey of the intestinal microbiota dynamics in offspring of hma mice over 18 weeks following weaning as compared to their mothers applying both cultural and molecular methods. Mice were maintained under standard hygienic conditions with open cages, handled under aseptic conditions, and fed autoclaved chow and water...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28386471/characterization-of-virulence-potential-of-pseudomonas-aeruginosa-isolated-from-bovine-meat-fresh-fish-and-smoked-fish
#10
Comoé Koffi Donatien Benie, Adjéhi Dadié, Nathalie Guessennd, Nadège Ahou N'gbesso-Kouadio, N'zebo Désiré Kouame, David Coulibaly N'golo, Solange Aka, Etienne Dako, Koffi Marcellin Dje, Mireille Dosso
Pseudomonas aeruginosa owns a variability of virulence factors. These factors can increase bacterial pathogenicity and infection severity. Despite the importance of knowledge about them, these factors are not more characterized at level of strains derived from local food products. This study aimed to characterize the virulence potential of P. aeruginosa isolated from various animal products. Several structural and virulence genes of P. aeruginosa including lasB, exoS, algD, plcH, pilB, exoU, and nan1 were detected by polymerase chain reaction (PCR) on 204 strains of P...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28386470/determining-the-risk-of-intra-community-transmission-of-tuberculosis-in-the-northwest-of-iran-through-15-loci-miru-vntr-typing
#11
Ali Afaghi-Gharamaleki, Seyyedreza Moaddab, Mojtaba Darbouy, Khalil Ansarin, Shahram Hanifian
This study was carried out in order to investigating the effect of travelling on the transmission of tuberculosis from high- to low-burden TB countries. Mycobacteria samples isolated from patients of distinct and relatively co-related countries (Azerbaijan Republic and Tabriz [located in the northwest of Iran]) were analyzed through 15 loci MIRU-VNTR typing method. PCR was done using special primers for each of the loci; then the number of allele repeats for all loci were determined by the size of their fragments...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28386469/propionibacterium-acnes-phylogenetic-type-iii-is-associated-with-progressive-macular-hypomelanosis
#12
Rolf L W Petersen, Christian F P Scholz, Anders Jensen, Holger Brüggemann, Hans B Lomholt
Progressive macular hypomelanosis (PMH) is a skin disorder that is characterized by hypopigmented macules and usually seen in young adults. The skin microbiota, in particular the bacterium Propionibacterium acnes, is suggested to play a role. Here, we compared the P. acnes population of 24 PMH lesions from eight patients with corresponding nonlesional skin of the patients and matching control samples from eight healthy individuals using an unbiased, culture-independent next-generation sequencing approach. We also compared the P...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28386468/comparison-of-mast-burkholderia-cepacia-ashdown-gentamicin-and-burkholderia-pseudomallei-selective-agar-for-the-selective-growth-of-burkholderia-spp
#13
Carola Edler, Henri Derschum, Mirko Köhler, Heinrich Neubauer, Hagen Frickmann, Ralf Matthias Hagen
Reliable identification of pathogenic Burkholderia spp. like Burkholderia mallei and Burkholderia pseudomallei in clinical samples is desirable. Three different selective media were assessed for reliability and selectivity with various Burkholderia spp. and nontarget organisms. Mast Burkholderia cepacia agar, Ashdown + gentamicin agar, and B. pseudomallei selective agar were compared. A panel of 116 reference strains and well-characterized clinical isolates, comprising 30 B. pseudomallei, 20 B. mallei, 18 other Burkholderia spp...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28386467/microbiota-composition-and-immune-responses-during-campylobacter-jejuni-infection-in-conventionally-colonized-il-10-mice-lacking-nucleotide-oligomerization-domain-2
#14
Markus M Heimesaat, Ursula Grundmann, Marie E Alutis, André Fischer, Stefan Bereswill
Host immune responses are pivotal for combating enteropathogenic infections. We here assessed the impact of the innate receptor nucleotide oligomerization domain protein 2 (NOD2) in murine Campylobacter jejuni-infection. Conventionally colonized IL-10(-/-) mice lacking NOD2 and IL-10(-/-) controls were perorally challenged with C. jejuni strain 81-176 and displayed comparable pathogenic colonization of intestines until day 14 postinfection (p.i.). Whereas overall intestinal microbiota compositions were comparable in naive mice, NOD2(-/-) IL-10(-/-) mice exhibited less fecal bifidobacteria and lactobacilli than IL-10(-/-) counterparts after infection...
March 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27980859/usage-of-murine-t-cell-hybridoma-cells-as-responder-cells-reveals-interference-of-helicobacter-pylori-with-human-dendritic-cell-mediated-antigen-presentation
#15
Michael Fehlings, Lea Drobbe, Macarena Beigier-Bompadre, Pablo Renner Viveros, Verena Moos, Thomas Schneider, Thomas F Meyer, Toni Aebischer, Ralf Ignatius
Direct effects of Helicobacter pylori (H. pylori) on human CD4(+) T-cells hamper disentangling a possible bacterial-mediated interference with major histocompatibility complex class II (MHC-II)-dependent antigen presentation to these cells. To overcome this limitation, we employed a previously described assay, which enables assessing human antigen-processing cell function by using murine T-cell hybridoma cells restricted by human leukocyte antigen (HLA) alleles. HLA-DR1(+) monocyte-derived dendritic cells were exposed to H...
December 1, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27980858/influence-of-toxoplasma-gondii-infection-on-symptoms-and-signs-of-premenstrual-syndrome-a-cross-sectional-study
#16
Cosme Alvarado-Esquivel, Luis Francisco Sánchez-Anguiano, Jesús Hernández-Tinoco, Alma Rosa Pérez-Álamos, Yazmin Del Rosario Rico-Almochantaf, Sergio Estrada-Martínez, Raquel Vaquera-Enriquez, Arturo Díaz-Herrera, Agar Ramos-Nevarez, Ada Agustina Sandoval-Carrillo, José Manuel Salas-Pacheco, Sandra Margarita Cerrillo-Soto, Elizabeth Irasema Antuna-Salcido, Oliver Liesenfeld, Carlos Alberto Guido-Arreola
Infection with Toxoplasma gondii in brain may cause some symptoms that resemble those in women with premenstrual syndrome. To determine the association of T. gondii infection with symptoms and signs of premenstrual syndrome, we examined 489 women aged 30-40 years old. Sera of participants were analyzed for the presence of anti-Toxoplasma IgG and IgM antibodies using enzyme-linked immunoassays (EIA) and T. gondii DNA by polymerase chain reaction (PCR). Anti-T. gondii IgG antibodies were found in 38 (7.8%) of the women studied...
December 1, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27980857/comparative-antibody-responses-against-three-antimalarial-vaccine-candidate-antigens-from-urban-and-rural-exposed-individuals-in-gabon
#17
Roméo-Karl Imboumy-Limoukou, Sandrine Lydie Oyegue-Liabagui, Stella Ndidi, Irène Pegha-Moukandja, Charlene Lady Kouna, Francis Galaway, Isabelle Florent, Jean Bernard Lekana-Douki
The analysis of immune responses in diverse malaria endemic regions provides more information to understand the host's immune response to Plasmodium falciparum. Several plasmodial antigens have been reported as targets of human immunity. PfAMA1 is one of most studied vaccine candidates; PfRH5 and Pf113 are new promising vaccine candidates. The aim of this study was to evaluate humoral response against these three antigens among children of Lastourville (rural area) and Franceville (urban area). Malaria was diagnosed using rapid diagnosis tests...
December 1, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27980856/effect-of-rpon-rpos-and-luxs-pathways-on-the-biofilm-formation-and-antibiotic-sensitivity-of-borrelia-burgdorferi
#18
Eva Sapi, Priyanka A S Theophilus, Truc V Pham, Divya Burugu, David F Luecke
Borrelia burgdorferi, the causative agent of Lyme disease, is capable of forming biofilm in vivo and in vitro, a structure well known for its resistance to antimicrobial agents. For the formation of biofilm, signaling processes are required to communicate with the surrounding environment such as it was shown for the RpoN-RpoS alternative sigma factor and for the LuxS quorum-sensing pathways. Therefore, in this study, the wild-type B. burgdorferi and different mutant strains lacking RpoN, RpoS, and LuxS genes were studied for their growth characteristic and development of biofilm structures and markers as well as for their antibiotic sensitivity...
December 1, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27980855/dissecting-the-interplay-between-intestinal-microbiota-and-host-immunity-in-health-and-disease-lessons-learned-from-germfree-and-gnotobiotic-animal-models
#19
REVIEW
Ulrike Fiebiger, Stefan Bereswill, Markus M Heimesaat
This review elaborates the development of germfree and gnotobiotic animal models and their application in the scientific field to unravel mechanisms underlying host-microbe interactions and distinct diseases. Strictly germfree animals are raised in isolators and not colonized by any organism at all. The germfree state is continuously maintained by birth, raising, housing and breeding under strict sterile conditions. However, isolator raised germfree mice are exposed to a stressful environment and exert an underdeveloped immune system...
December 1, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766174/comparison-of-five-commercial-nucleic-acid-extraction-kits-for-the-pcr-based-detection-of-burkholderia-pseudomallei-dna-in-formalin-fixed-paraffin-embedded-tissues
#20
Sonja Obersteller, Heinrich Neubauer, Ralf Matthias Hagen, Hagen Frickmann
The extraction and further processing of nucleic acids (NA) from formalin-fixed paraffin-embedded (FFPE) tissues for microbiological diagnostic polymerase chain reaction (PCR) approaches is challenging. Here, we assessed the effects of five different commercially available nucleic acid extraction kits on the results of real-time PCR. FFPE samples from organs of Burkholderia pseudomallei-infected Swiss mice were subjected to processing with five different extraction kits from QIAGEN (FFPE DNA Tissue Kit, EZ1 DNA Tissue Kit, DNA Mini Kit, DNA Blood Mini Kit, and FlexiGene DNA Kit) in combination with three different real-time PCRs targeting B...
September 29, 2016: European Journal of Microbiology & Immunology
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