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European Journal of Microbiology & Immunology

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https://www.readbyqxmd.com/read/27766174/comparison-of-five-commercial-nucleic-acid-extraction-kits-for-the-pcr-based-detection-of-burkholderia-pseudomallei-dna-in-formalin-fixed-paraffin-embedded-tissues
#1
Sonja Obersteller, Heinrich Neubauer, Ralf Matthias Hagen, Hagen Frickmann
The extraction and further processing of nucleic acids (NA) from formalin-fixed paraffin-embedded (FFPE) tissues for microbiological diagnostic polymerase chain reaction (PCR) approaches is challenging. Here, we assessed the effects of five different commercially available nucleic acid extraction kits on the results of real-time PCR. FFPE samples from organs of Burkholderia pseudomallei-infected Swiss mice were subjected to processing with five different extraction kits from QIAGEN (FFPE DNA Tissue Kit, EZ1 DNA Tissue Kit, DNA Mini Kit, DNA Blood Mini Kit, and FlexiGene DNA Kit) in combination with three different real-time PCRs targeting B...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766173/multidrug-resistance-of-acinetobacter-baumannii-in-ladoke-akintola-university-teaching-hospital-osogbo-nigeria
#2
G Odewale, O J Adefioye, J Ojo, F A Adewumi, O A Olowe
Acinetobacter baumannii is a ubiquitous pathogen that has emerged as a major cause of healthcare-associated infections at Ladoke Akintola University Teaching Hospital. Isolates were assayed according to standard protocol. The isolates were subjected to molecular techniques to detect blaOXA, blaTEM, blaCTX-M, and blaSHV genes in strains of the A. baumannii isolates. The prevalence of A. baumannii was 8.5% and was most prevalent among patients in the age group 51-60 (36%); the male patients (63.6%) were more infected than their female counterparts...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766172/risk-reduction-of-needle-stick-injuries-due-to-continuous-shift-from-unsafe-to-safe-instruments-at-a-german-university-hospital
#3
Hagen Frickmann, Wibke Schmeja, Emil Reisinger, Thomas Mittlmeier, Karen Mitzner, Norbert Georg Schwarz, Philipp Warnke, Andreas Podbielski
This study assessed protective effects of a continuous introduction of safe instruments in terms of reduction of needle stick injuries. The retrospective study analyzed correlations between the increasing proportion of safe instruments and a reduction of the incidence of needle stick injuries linked to such instruments in a German university hospital over 5 years. Incidents declined about 17.6% from 80.3 incidents per 1000 employees to 66.2, associated with an increase in the proportions of injuries due to instruments without protective mechanisms such as scalpels or hypodermic needles by 12...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766171/tissue-specific-induction-of-ccr6-and-nrp1-during-early-cd4-t-cell-differentiation
#4
Joern Pezoldt, Jochen Huehn
Upon differentiation, T cells acquire tissue-specific homing properties allowing efficient targeting of effector T cells into distinct inflamed organs. Priming of T cells within skin-draining, peripheral lymph nodes (pLNs) leads to the expression of E- and P-selectin ligands, which facilitate migration into inflamed skin, whereas activation within gut-draining, mesenteric LNs (mLNs) results in induction of chemokine receptor CCR9 and integrin α4β7, both required for migration of effector T cells into mucosal tissues...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766170/laboratory-experience-with-the-liaison-analyzer-in-the-diagnosis-of-clostridium-difficile-associated-diarrhea
#5
Orsolya Benedek, Andreas Podbielski, Philipp Warnke
BACKGROUND: Chemiluminescent or enzyme-linked fluorescent immunoassays are commonly used to diagnose Clostridium difficile-associated diarrhea. METHODS: The LIAISON analyzer (DiaSorin, Italy) was compared to miniVIDAS (bioMérieux, France) and, furthermore, to culture of toxigenic strains. In total, 249 native stool samples were analyzed. Sensitivities, specificities, and positive and negative predictive values were investigated. Furthermore, performance under routine conditions was assessed...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766169/microbial-etiology-of-community-acquired-pneumonia-among-infants-and-children-admitted-to-the-pediatric-hospital-ain-shams-university
#6
Magda Yehia El Seify, Eman Mahmoud Fouda, Hanan Mohamed Ibrahim, Maha Muhammad Fathy, Asmaa Al Husseiny Ahmed, Walaa Shawky Khater, Noha Nagi Mohammed Salah El Deen, Heba Galal Mohamed Abouzeid, Nancy Riyad Ahmed Hegazy, Heba Salah Sayed Elbanna
BACKGROUND: While recognizing the etiology of community-acquired pneumonia is necessary for formulating local antimicrobial guidelines, limited data is published about this etiology in Egyptian pediatric patients. OBJECTIVES: To determine the frequency of bacterial and viral pathogens causing community-acquired pneumonia (CAP) among immunocompetent Egyptian infants and preschool children. METHODS: Ninety infants and preschool-age children admitted to our hospital with CAP were prospectively included in the study...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766168/molecular-differentiation-of-shigella-spp-from-enteroinvasive-e-coli
#7
I Løbersli, A L Wester, Å Kristiansen, L T Brandal
A real-time polymerase chain reaction (PCR) assay, amplifying the genes encoding lactose permease (lacY) and invasion plasmid antigen H (ipaH), was run on 121 isolates phenotypically classified as Shigella spp., enteroinvasive Escherichia coli (EIEC), or EIEC O nontypable (ONT). The results were compared with data from a generic E. coli multiple-locus variable-number of tandem repeat analysis (MLVA) and a Shigella MLVA. The real-time PCR verified all Shigella spp. (n = 53) as Shigella (lacY negative) and all EIEC O121 (n = 15) and EIEC O124 (n = 2) as EIEC (lacY positive)...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766167/a-c-terminal-coiled-coil-region-of-cagl-is-responsible-for-helicobacter-pylori-induced-il-8-expression
#8
Tobias Wiedemann, Stefan Hofbaur, Eva Loell, Gabriele Rieder
Interleukin-8 (IL-8) is a potent neutrophil-activating chemokine which triggers the infiltration and migration of neutrophils into areas of bacterial infection. Helicobacter pylori-infected patient studies as well as animal models have revealed that H. pylori type I strains carrying an intact cytotoxin-associated gene pathogenicity island (cag-PAI) with a functional type IV secretion system (T4SS) induce IL-8 expression and secretion in gastric mucosa. This gastric mucosal IL-8 expression correlates with severe histological changes due to H...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766166/role-of-supar-and-lactic-acid-in-diagnosing-sepsis-and-predicting-mortality-in-elderly-patients
#9
Walaa S Khater, Noha N Salah-Eldeen, Mohamed S Khater, Asghraf N Saleh
This study investigated the diagnostic value of soluble urokinase plasminogen activator receptor (suPAR) and serum lactate in elderly patients with sepsis and evaluated their capacity to predict mortality and their correlation to Sequential Organ Failure Assessment (SOFA) score. The study included 80 participants, divided into two groups: 40 cases (mean age, 68.9 ± 5.9) admitted to the intensive care unit and 40 healthy controls (mean age, 67.1 ± 6.2). Elderly patients with sepsis had significantly higher levels of serum suPAR and lactic acid compared to healthy controls...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766165/association-of-campylobacter-jejuni-ssp-jejuni-chemotaxis-receptor-genes-with-multilocus-sequence-types-and-source-of-isolation
#10
Norah Lynn-Anne Mund, Wycliffe Omurwa Masanta, Anne-Marie Goldschmidt, Raimond Lugert, Uwe Groß, Andreas E Zautner
Campylobacter jejuni's flagellar locomotion is controlled by eleven chemoreceptors. Assessment of the distribution of the relevant chemoreceptor genes in the C. jejuni genomes deposited in the National Center for Biotechnology Information (NCBI) database led to the identification of two previously unknown tlp genes and a tlp5 pseudogene. These two chemoreceptor genes share the same locus in the C. jejuni genome with tlp4 and tlp11, but the gene region encoding the periplasmic ligand binding domain differs significantly from other chemoreceptor genes...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27766164/insights-from-100-years-of-research-with-probiotic-e-coli
#11
Trudy M Wassenaar
A century ago, Alfred Nissle discovered that intentional intake of particular strains of Escherichia coli could treat patients suffering from infectious diseases. Since then, one of these strains became the most frequently used probiotic E. coli in research and was applied to a variety of human conditions. Here, properties of that E. coli Nissle 1917 strain are compared with other commercially available E. coli probiotic strains, with emphasis on their human applications. A literature search formed the basis of a summary of research findings reported for the probiotics Mutaflor, Symbioflor 2, and Colinfant...
September 29, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27429796/colonic-expression-of-genes-encoding-inflammatory-mediators-and-gelatinases-during-campylobacter-jejuni-infection-of-conventional-infant-mice
#12
Markus M Heimesaat, Ursula Grundmann, Marie E Alutis, André Fischer, Ulf B Göbel, Stefan Bereswill
Within 1 week following peroral Campylobacter jejuni infection, infant mice develop acute enteritis resolving thereafter. We here assessed colonic expression profiles of mediators belonging to the IL-23/IL-22/IL-18 axis and of matrix-degrading gelatinases MMP-2 and MMP-9 at day 6 post C. jejuni strain 81-176 infection. Whereas the pathogen readily colonized the intestines of infant IL-18(-/-) mice only, colonic mucin-2 mRNA, a pivotal mucus constituent, was downregulated in IL-22(-/-) mice and accompanied by increased expression of pro-inflammatory cytokines including IFN-γ, TNF, IL-17A, and IL-1β...
June 24, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27429795/the-role-of-il-23-il-22-and-il-18-in-campylobacter-jejuni-infection-of-conventional-infant-mice
#13
Markus M Heimesaat, Marie E Alutis, Ursula Grundmann, André Fischer, Ulf B Göbel, Stefan Bereswill
We have recently shown that, within 1 week following peroral Campylobacter jejuni infection, conventional infant mice develop self-limiting enteritis. We here investigated the role of IL-23, IL-22, and IL-18 during C. jejuni strain 81-176 infection of infant mice. The pathogen efficiently colonized the intestines of IL-18(-/-) mice only, but did not translocate to extra-intestinal compartments. At day 13 postinfection (p.i.), IL-22(-/-) mice displayed lower colonic epithelial apoptotic cell numbers as compared to wildtype mice, whereas, conversely, colonic proliferating cells increased in infected IL-22(-/-) and IL-18(-/-) mice...
June 24, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27429794/seroprevalence-of-campylobacter-specific-antibodies-in-two-german-duck-farms-a-prospective-follow-up-study
#14
Wycliffe Omurwa Masanta, Raimond Lugert, Uwe Groß, Gunter Linsel, Astrid Heutelbeck, Andreas Erich Zautner
Several studies have shown that about 60-100% of farmed ducks are colonized by Campylobacter species. Because of this, a higher risk of campylobacteriosis among duck farm workers can be assumed. To estimate the risk of Campylobacter infections in duck farm workers, we investigated the prevalence of Campylobacter spp. in ducks of two duck farms and the seroprevalence of anti-Campylobacter antibodies (IgA and IgG) in two cohorts of workers. The first cohort consisted of high-exposed stable workers and slaughterers, which was compared to a second cohort of non-/low-exposed persons...
June 24, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27429793/molecular-epidemiology-of-carbapenem-resistant-acinetobacter-baumannii-complex-isolates-from-patients-that-were-injured-during-the-eastern-ukrainian-conflict
#15
Heike Granzer, Ralf Matthias Hagen, Philipp Warnke, Wolfgang Bock, Tobias Baumann, Norbert Georg Schwarz, Andreas Podbielski, Hagen Frickmann, Thomas Koeller
This study addressed carbapenem-resistant Acinetobacter baumannii complex (ABC) isolates from patients that were injured during the military conflict in the Eastern Ukraine and treated at German Armed Forces Hospitals in 2014 and 2015. Clonal diversity of the strains and potential ways of transmission were analyzed. Patients with one or several isolation events of carbapenem-resistant ABC were included. Isolates were characterized by VITEK II-based identification and resistance testing, molecular screening for frequent carbapenemase genes, and DiversiLab rep-PCR-based typing...
June 24, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27429792/microrna-response-of-primary-human-macrophages-to-arcobacter-butzleri-infection
#16
Jennifer Zur Bruegge, Christina Backes, Greta Gölz, Georg Hemmrich-Stanisak, Lydia Scharek-Tedin, Andre Franke, Thomas Alter, Ralf Einspanier, Andreas Keller, Soroush Sharbati
The role of microRNAs (miRNAs) in infectious diseases is becoming more and more apparent, and the use of miRNAs as a diagnostic tool and their therapeutic application has become the major focus of investigation. The aim of this study was to identify miRNAs involved in the immune signaling of macrophages in response to Arcobacter (A.) butzleri infection, an emerging foodborne pathogen causing gastroenteritis. Therefore, primary human macrophages were isolated and infected, and miRNA expression was studied by means of RNAseq...
June 24, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27429791/seroepidemiology-of-toxoplasma-gondii-infection-in-people-applying-for-medical-certificates
#17
Cosme Alvarado-Esquivel, Luis Francisco Sánchez-Anguiano, Jesús Hernández-Tinoco, Rosa Angélica Pulido-Montoya, Guadalupe Acosta-Rojas, Sergio Estrada-Martínez, Alma Rosa Pérez-Álamos, Raquel Vaquera-Enriquez, Arturo Díaz-Herrera, Raúl Segura-Moreno, María de Lourdes Guerrero-Carbajal, Oliver Liesenfeld, Isabel Beristain García, María Guadalupe Rentería-López
Medical certificates are documents that state the health status of a person. This study aimed to determine the seroprevalence and risk factors for Toxoplasma gondii infection in applicants of medical certificates and to investigate seroprevalence association with characteristics of these individuals. We examined 404 applicants in a public health center in Durango City, Mexico for the presence of anti-Toxoplasma IgG and IgM antibodies using enzyme-linked immunoassays. Of the 404 subjects (mean age of 35.94 ± 13...
June 24, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27429790/toxoplasma-gondii-infection-and-depression-a-case-control-seroprevalence-study
#18
Cosme Alvarado-Esquivel, Luis Francisco Sánchez-Anguiano, Jesús Hernández-Tinoco, Luis Omar Berumen-Segovia, Yazmin Elizabeth Torres-Prieto, Sergio Estrada-Martínez, Alma Rosa Pérez-Álamos, María Nalleli Ortiz-Jurado, Gabriel Molotla-de-León, Isabel Beristain-García, Elizabeth Rábago-Sánchez, Oliver Liesenfeld
We assessed the association of Toxoplasma gondii infection and depression in a sample of psychiatric patients and control subjects without depression. We performed an age- and gender-matched case-control study of 89 patients suffering from depression attended in a public psychiatric hospital in Durango City, Mexico and 356 control subjects without depression from the general population of the same city. Participants were tested for the presence of anti-Toxoplasma IgG and IgM antibodies using enzyme-linked immunoassays...
June 24, 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27141317/recurrent-invasive-pneumococcal-disease-serotype-12f-in-a-vaccinated-splenectomized-patient
#19
Anne Katrine Blaabjerg, Anna Holst Schumacher, Bjørn Kantsø, Lena Hagelskjær Kristensen, Helga Schumacher
This is the first case report of recurrent invasive pneumococcal disease (IPD), specifically, due to serotype 12F. The patient described here was vaccinated with the 23-valent pneumococcal polysaccharide vaccine (PPV23) due to previous splenectomy, and an anti-pneumococcal IgG test concluded that she had responded sufficiently to vaccination. Still, she had a fulminate recurrent infection with PPV23 serotype 12F. We investigated the anti-pneumococcal IgG test, and it turned out that it is based on the geometric mean value of only 12 of the serotypes included in PPV23; 12F is none of them...
March 2016: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/27141316/toll-like-receptor-4-dependent-intestinal-gene-expression-during-arcobacter-butzleri-infection-of-gnotobiotic-il-10-deficient-mice
#20
Greta Gölz, Thomas Alter, Stefan Bereswill, Markus M Heimesaat
We have previously shown that Arcobacter butzleri infection induces Toll-like receptor (TLR) -4 dependent immune responses in perorally infected gnotobiotic IL-10(-/-) mice. Here, we analyzed TLR-4-dependent expression of genes encoding inflammatory mediators and matrix-degrading gelatinases MMP-2 and -9 in the small and large intestines of gnotobiotic TLR-4-deficient IL-10(-/-) mice that were perorally infected with A. butzleri strains CCUG 30485 or C1, of human and chicken origin, respectively. At day 6 following A...
March 2016: European Journal of Microbiology & Immunology
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