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European Journal of Microbiology & Immunology

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https://www.readbyqxmd.com/read/29403660/equivalent-performance-of-the-cobas%C3%A2-cdiff-test-for-use-on-the-cobas%C3%A2-liat%C3%A2-system-and-the-cobas%C3%A2-4800-system
#1
Sachin K Garg, Kyle Lu, John Duncan, Lance R Peterson, Oliver Liesenfeld
Clostridium difficile infection is a significant health burden, and innovative solutions are needed to shorten time to diagnosis and improve infection control. We evaluated the performance of the cobas® Cdiff test for use on the cobas® Liat® System (cobas® Liat® Cdiff), a single-sample, on-demand, and automated molecular solution with a 20-min turnaround time. The limit of detection was 45-90 colony-forming units (CFUs)/swab for toxigenic strains that covered the most prevalent toxinotypes, including the hyper-virulent epidemic 027/BI/NAP1 strain...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29403659/toxoplasma-gondii-exposure-and-neurological-disorders-an-age-and-gender-matched-case-control-pilot-study
#2
Cosme Alvarado-Esquivel, Yazmin Del Rosario Rico-Almochantaf, Jesús Hernández-Tinoco, Gerardo Quiñones-Canales, Luis Francisco Sánchez-Anguiano, Jorge Torres-González, Björn Schott, Oliver Liesenfeld, Ildiko Rita Dunay
Little is known about the association of Toxoplasma gondii infection and neurological disorders. We performed a case-control study with 344 patients with neurological diseases and 344 neurologically healthy age- and gender-matched subjects. Sera of participants were analyzed for anti-T. gondii IgG and IgM antibodies using commercially available immunoassays. Anti-T. gondii IgG antibodies were detected in 25 (7.3%) cases and in 35 (10.2%) controls (odds ratio [OR] = 0.69; 95% confidence interval [CI]: 0.40-1...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29403658/antimicrobial-resistance-transducing-bacteriophages-isolated-from-surfaces-of-equine-surgery-clinics-a-pilot-study
#3
Miriam Hilbert, Isabella Csadek, Ulrike Auer, Friederike Hilbert
In the past, the horizontal transfer of antimicrobial resistance genes was mainly associated with conjugative plasmids or transposons, whereas transduction by bacteriophages was thought to be a rare event. In order to analyze the likelihood of transduction of antimicrobial resistance in the field of clinical veterinary medicine, we isolated phages from Escherichia coli from a surgery suite of an equine clinic. In a pilot study, the surgery suite of a horse clinic was sampled directly after surgery and subsequently sampled after cleaning and disinfection following a sampling plan based on hygiene, surgery, and anesthesia...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29403657/on-the-role-of-enterococci-in-the-bloodstream-results-of-a-single-center-retrospective-observational-study-at-a-german-university-hospital
#4
Hagen Frickmann, Kerstin Köller, Irina Veil, Mirjam Weise, Alicja Ludyga, Norbert Georg Schwarz, Philipp Warnke, Andreas Podbielski
This study assesses the clinical relevance of vancomycin-susceptible enterococci in bacteremic patients and compares it with bacteremia due to Staphylococcus aureus and Escherichia coli. During a 5-year-study interval, clinical and diagnostic features of patients with enterococcal bacteremia were compared to those of patients with E. coli or S. aureus bacteremia. Each patient was only counted once per hospital stay. During the 5-year study interval, data from 267 patients with enterococcal bacteremia and from 661 patients with bacteremia due to E...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29403656/seroepidemiology-of-infection-with-neospora-caninum-leptospira-and-bovine-herpesvirus-type-1-in-water-buffaloes-bubalus-bubalis-in-veracruz-mexico
#5
Dora Romero-Salas, Cosme Alvarado-Esquivel, Gladys Domínguez-Aguilar, Anabel Cruz-Romero, Nelly Ibarra-Priego, Carolina Barrientos-Salcedo, Mariel Aguilar-Domínguez, Rodolfo Canseco-Sedano, Luz Teresa Espín-Iturbe, Luis Francisco Sánchez-Anguiano, Jesús Hernández-Tinoco, Adalberto A Pérez de León
We aimed to determine the seroprevalence of infection with Neospora caninum, Leptospira , and bovine herpesvirus type 1 and risk factors associated with these infections in water buffaloes in Veracruz State, Mexico. Through a cross-sectional study, 144 water buffaloes (Bubalus bubalis) raised in 5 ranches of Veracruz were examined for anti-N. caninum and anti-bovine herpesvirus type 1 antibodies by enzyme immunoassays, and anti-Leptospira interrogans antibodies by microscopic agglutination test. Of the 144 buffaloes studied, 35 (24...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29403655/influence-of-broth-enrichment-as-well-as-storage-and-transport-time-on-the-sensitivity-of-mrsa-surveillance-in-the-tropics
#6
Hagen Frickmann, Andreas Hahn, Norbert Georg Schwarz, Ralf Matthias Hagen, Denise Dekker, Rebecca Hinz, Volker Micheel, Benedikt Hogan, Jürgen May, Raphael Rakotozandrindrainy
Direct growth on blood and screening agar for methicillin-resistant Staphylococcus aureus (MRSA) at a tropical surveillance site was compared with broth enrichment and subsequent growth on selective MRSA agar after international sample transport. In Madagascar, 1548 swabs from an MRSA surveillance study were assessed for growth on Columbia blood agar enriched with 5% sheep blood and MRSA screening agar at the surveillance site with subsequent cold storage of the samples and shipment to Germany. In Germany, 1541 shipped samples were analyzed by non-selective broth enrichment with subsequent culture on MRSA selective agar...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29403654/seroprevalence-of-chlamydophila-psittaci-among-employees-of-two-german-duck-farms
#7
Raimond Lugert, Uwe Groß, Wycliffe O Masanta, Gunter Linsel, Astrid Heutelbeck, Andreas E Zautner
Psittacosis is a zoonotic infectious disease that is caused by Chlamydophila psittaci. To determine the occupational risk of getting the infection, we investigated the seroprevalence of C. psittaci among employees of two German duck farms and two slaughterhouses according to their level of exposure to the pathogen during the years 2010, 2007, and 2004. In summary, we found low seroprevalence (≈ 8%) throughout the study population almost irrespective of the duty of a given worker. Surprisingly, in 2010, the anti-C...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29403653/prevalence-of-gram-negative-bacilli-isolated-from-the-equipment-and-surfaces-in-hospital-wards-of-golestan-province-north-of-iran
#8
Ali Asghar Ayatollahi, Abolfazl Amini, Somayeh Rahimi, Saeid Rahbar Takrami, Reza Kazemi Darsanaki, Muhammad Sadeqi Nezhad
Nosocomial infections are one of the most common causes of death in hospitals. This study aimed to determine the prevalence of gram-negative bacilli isolated from the equipment in hospital wards of the Golestan province, in the year 2015. In this cross-sectional study in 2015, 1980 samples from medical and nonmedical equipment and surfaces were collected from the wards of 13 teaching hospitals, in the Golestan province. Samples were inoculated into eosin methylene blue agar and blood agar culture media and isolated colonies were identified by standard biochemical tests...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29403652/high-level-of-specific-anti-plasmodium-falciparum-merozoite-igg1-antibodies-in-rural-asymptomatic-individuals-of-dienga-south-eastern-gabon
#9
Irène Pegha-Moukandja, Roméo-Karl Imboumy-Limoukou, Nina Tchitoula-Makaya, Augustin-Ghislain Mouinga-Ondeme, Jean Claude Biteghe-Bi-Essone, Dieudonne Nkoghe Mba, Jean-Bernard Lekana-Douki, Fousseyni S Toure Ndouo
Plasmodium falciparum merozoite antigens (PfMAgs) play an essential role in the development of immunity to malaria. Currently, P. falciparum: protein 113 (Pf 113), apical membrane antigen 1 (AMA1), erythrocyte binding antigens (EBA175), and reticulocyte binding protein homologue 5 (RH5) are among the most PfMAgs studied. A comparative analysis of naturally acquired antibodies against these antigens in children would increase our knowledge about the development of protective immunity. Analysis of antibodies to Pf113, PfAMA1, PfEBA175, and PfRH5 was conducted in rural population during 2013 and 2014...
December 18, 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034113/low-neurotoxicity-of-onx-0914-supports-the-idea-of-specific-immunoproteasome-inhibition-as-a-side-effect-limiting-therapeutic-strategy
#10
Laura von Brzezinski, Paula Säring, Peter Landgraf, Clemens Cammann, Ulrike Seifert, Daniela C Dieterich
Application of the proteasome inhibitor Bortezomib for the treatment of haematopoietic malignancies such as multiple myeloma significantly improves the average overall survival of patients. However, one of the most severe side effects is the development of peripheral neuropathies caused by neurotoxic effects of Bortezomib limiting its therapeutic efficacy. With ONX-0914 a specific inhibitor of the β5i (LMP7)-immunosubunit containing proteasomes was developed that targets exclusively the proteasome subtypes mainly expressed in immune cells including B lymphocytes as the origin of multiple myeloma...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034112/lack-of-association-between-cytomegalovirus-infection-and-hypertensive-disorders-in-pregnancy-a-case-control-study-in-durango-mexico
#11
Cosme Alvarado-Esquivel, Ada A Sandoval-Carrillo, Fernando Vazquez-Alaniz, José M Salas-Pacheco, Jesús Hernández-Tinoco, Luis Francisco Sánchez-Anguiano, Elizabeth Irasema Antuna-Salcido
It is not clear whether infection with cytomegalovirus (CMV) is associated with hypertensive disorders in pregnant women. Through a case-control study design, 146 women suffering from hypertensive disorders in pregnancy (cases) and 146 age-matched normotensive pregnant women (controls) were examined for the presence of anti-CMV IgG and IgM antibodies with enzyme-linked immunoassays. IgM seropositive samples were further assayed by enzyme-linked fluorescent assay (ELFA). Anti-CMV IgG antibodies were found in 138 (94...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034111/molecular-characterization-of-diarrheagenic-escherichia-coli-in-children-less-than-5-years-of-age-with-diarrhea-in-ouagadougou-burkina-faso
#12
Ali Konaté, René Dembélé, Assèta Kagambèga, Issiaka Soulama, Wendpoulomdé A D Kaboré, Emmanuel Sampo, Haoua Cissé, Antoine Sanou, Samuel Serme, Soumanaba Zongo, Cheikna Zongo, Alio Mahamadou Fody, Nathalie K Guessennd, Alfred S Traoré, Amy Gassama-Sow, Nicolas Barro
Diarrheagenic Escherichia coli (DEC) is important bacteria of children's endemic and epidemic diarrhea worldwide. The aim of this study was to determine the prevalence of DEC isolated from stool samples collected from children with acute diarrhea living in Ouagadougou, Burkina Faso. From August 2013 to October 2015, stool samples were collected from 315 children under 5 years of age suffering from diarrhea in the "Centre Médical avec Antenne Chirurgicale (CMA)" Paul VI and the CMA of Schiphra. E. coli were isolated and identified by standard microbiological methods, and the 16-plex PCR method was used to further characterize them...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034110/toll-like-receptor-4-dependent-inflammatory-responses-following-intestinal-colonization-of-secondary-abiotic-il10-deficient-mice-with-multidrug-resistant-pseudomonas-aeruginosa
#13
Anne Grunau, Ulrike Escher, Stefan Bereswill, Markus M Heimesaat
The rising incidences of infections with multidrug-resistant (MDR) Gram-negative bacteria including Pseudomonas aeruginosa (PA) have gained increasing attention in medicine, but also in the general public and global health politics. The mechanisms underlying opportunistic pathogen-host interactions are unclear, however. To address this, we challenged secondary abiotic IL10(-/-) mice deficient for Toll-like receptor-4 (TLR4(-/-) × IL10(-/-)), the main receptor of the Gram-negative cell wall constituent lipopolysaccharide, with a clinical MDR PA isolate...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034109/multidrug-resistant-pseudomonas-aeruginosa-induce-systemic-pro-inflammatory-immune-responses-in-colonized-mice
#14
Eliane von Klitzing, Stefan Bereswill, Markus M Heimesaat
The World Health Organization has rated multidrug-resistant (MDR) Pseudomonas aeruginosa as a critical threat to human health. In the present study, we performed a survey of intestinal colonization, and local and systemic immune responses following peroral association of secondary abiotic mice with either a clinical MDR P. aeruginosa or a commensal murine Escherichia coli isolate. Depletion of the intestinal microbiota following antibiotic treatment facilitated stable intestinal colonization of both P. aeruginosa and E...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034108/intestinal-microbiota-changes-in-mice-lacking-pituitary-adenylate-cyclase-activating-polypeptide-pacap-bifidobacteria-make-the-difference
#15
Markus M Heimesaat, Gernot Reifenberger, Viktoria Vicena, Anita Illes, Gabriella Horvath, Andrea Tamas, Balazs D Fulop, Stefan Bereswill, Dora Reglodi
Pituitary adenylate cyclase activating polypetide (PACAP) constitutes a neuropeptide that is widely distributed in the host exerting essential cytoprotective properties, whereas PACAP(-/-) mice display increased susceptibility to distinct immunopathological conditions. The orchestrated interplay between the gut microbiota and the host is pivotal in immune homeostasis and resistance to disease. Potential pertubations of the intestinal microbiota in PACAP(-/-) mice, however, have not been addressed so far. For the first time, we performed a comprehensive survey of the intestinal microbiota composition in PACAP(-/-) and wildtype (WT) mice starting 2 weeks postpartum until 18 months of age applying quantitative culture-independent techniques...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034107/high-throughput-testing-of-urogenital-and-extragenital-specimens-for-detection-of-chlamydia-trachomatis-and-neisseria-gonorrhoeae-with-cobas-%C3%A2-ct-ng
#16
Elizabeth M Marlowe, David Hardy, Mark Krevolin, Peter Gohl, Alexander Bertram, Rodney Arcenas, Britta Seiverth, Tanja Schneider, Oliver Liesenfeld
We compared the analytical and clinical performance of cobas(®) CT/NG for use on the Cobas(®) 6800/8800 Systems with the Cobas(®) 4800 CT/NG Test from urogenital and extragenital specimens in over 12,000 specimens from both male and female subjects in Germany and the United States. The analytical sensitivity was ≤40 EB/ml for Chlamydia trachomatis (CT) and ≤1 CFU/ml for Neisseria gonorrhoeae (NG). Using clinical specimens, the overall percent agreement with the Cobas(®) 4800 CT/NG Test was >98.5%...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034106/epidemiology-and-antibiotic-resistance-phenotypes-of-diarrheagenic-escherichia-coli-responsible-for-infantile-gastroenteritis-in-ouagadougou-burkina-faso
#17
Ali Konaté, René Dembélé, Nathalie K Guessennd, Fernique Konan Kouadio, Innocent Kouamé Kouadio, Mohamed Baguy Ouattara, Wendpoulomdé A D Kaboré, Assèta Kagambèga, Haoua Cissé, Hadiza Bawa Ibrahim, Touwendsida Serge Bagré, Alfred S Traoré, Nicolas Barro
The emergence and persistence of multidrug-resistant (MDR) diarrheagenic Escherichia coli (DEC) causing acute diarrhea is a major public health challenge in developing countries. The aim of this study was to evaluate the resistance phenotypes of DEC isolated from stool samples collected from children less than 5 years of age with acute diarrhea living in Ouagadougou/Burkina Faso. From August 2013 to October 2015, this study was carried out on 31 DEC strains of our study conducted in "Centre Médical avec Antenne Chirurgicale (CMA)" Paul VI and CMA of Schiphra...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034105/changes-of-the-intestinal-microbiome-host-homeostasis-in-hiv-infected-individuals-a-focus-on-the-bacterial-gut-microbiome
#18
REVIEW
Ana Beatriz Dein Terra Mota Ribeiro, Markus M Heimesaat, Stefan Bereswill
Human immunodeficiency virus (HIV) infections cause severe CD4+ T cell depletion leading to chronic inflammation and immune activation, impaired barrier function, and microbial translocation. Even under effective antiretroviral therapy, these processes persist, leading to gut microbiome dysbiosis and disturbance of microbiome-host homeostasis. This systematic review aims at analyzing how gut microbiome and host immune system influence each other during HIV pathogenesis. An online search applying the PubMed database was conducted...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/29034104/fibroblasts-the-unknown-sentinels-eliciting-immune-responses-against-microorganisms
#19
REVIEW
Luis Antonio Bautista-Hernández, José Luis Gómez-Olivares, Beatriz Buentello-Volante, Victor Manuel Bautista-de Lucio
Fibroblasts are present in all tissues but predominantly in connective tissues. Some of their functions include contractility, locomotion, collagen and elastin fiber production, and the regulation and degradation of the extracellular matrix. Also, fibroblasts act as sentinels to produce inflammatory mediators in response to several microorganisms. There is evidence that fibroblasts can synthesize toll-like receptors (TLRs), antimicrobial peptides, proinflammatory cytokines, chemokines, and growth factors, which are important molecules involved in innate immune response against microorganisms...
September 2017: European Journal of Microbiology & Immunology
https://www.readbyqxmd.com/read/28690882/photoinactivation-of-legionella-rubrilucens-by-visible-light
#20
J Schmid, K Hoenes, M Rath, P Vatter, B Spellerberg, M Hessling
In this study, the photoinactivation of Legionella by visible light is investigated. The success of this approach would offer new prospects for technical water disinfection and maybe even for therapeutic measures in cases of Legionella infections. Therefore, Legionella rubrilucens was dispensed on buffered charcoal yeast extract medium agar plates and illuminated with different doses of violet light generated by 405 nm light-emitting diodes (LEDs). A strong photoinactivation effect was observed. A dose of 125 J/ cm(2) reduced the bacterial concentration by more than 5 orders of magnitude compared to Legionella on unirradiated agar plates...
June 2017: European Journal of Microbiology & Immunology
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