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Clinical and Experimental Reproductive Medicine

In Ae Cho, Yeon Jee Lee, Hee Jung Lee, In Young Choi, Jeong Kyu Shin, Soon Ae Lee, Jong Hak Lee, Won Jun Choi
Objective: The favored method of preserving fertility in young female cancer survivors is cryopreservation and autotransplantation of ovarian tissue. Reducing hypoxia until angiogenesis takes place is essential for the survival of transplanted ovarian tissue. The aim of this study was to investigate the role of angiopoietin-1 (Angpt-1), angiopoietin-2 (Angpt-2), and vascular endothelial growth factor (VEGF) in ovarian tissue grafts that were cryopreserved using two methods. Methods: Ovarian tissues harvested from ICR mice were divided into three groups: group I (control), no cryopreservation; group II, vitrification in EFS (ethylene-glycol, ficoll, and sucrose solution)-40; and group III, slow freezing in dimethyl sulfoxide...
September 2018: Clinical and Experimental Reproductive Medicine
Jae Jun Shin, Kyung Eui Park, Young Min Choi, Hye-Ok Kim, Dong-Hee Choi, Woo Sik Lee, Jung-Hyun Cho
Objective: To prospectively evaluate the efficacy and safety of a fixed early gonadotropin-releasing hormone (GnRH) antagonist protocol compared to a conventional midfollicular GnRH antagonist protocol and a long GnRH agonist protocol for in vitro fertilization (IVF) in patients with polycystic ovary syndrome (PCOS). Methods: Randomized patients in all three groups (early antagonist, n=14; conventional antagonist, n=11; long agonist, n=11) received 21 days of oral contraceptive pill treatment prior to stimulation...
September 2018: Clinical and Experimental Reproductive Medicine
Minh Tam Le, Thi Tam An Nguyen, Thi Thai Thanh Nguyen, Van Trung Nguyen, Dinh Duong Le, Vu Quoc Huy Nguyen, Ngoc Thanh Cao, Alar Aints, Andres Salumets
Objective: In frozen and thawed embryos, the zona pellucida (ZP) can be damaged due to hardening. Laser-assisted hatching (LAH) of embryos can increase the pregnancy rate. This study compared thinning and drilling of the ZP before frozen embryo transfer (FET). Methods: Patients were randomly allocated into two groups for LAH using thinning or drilling on day 2 after thawing. Twenty-five percent of the ZP circumference and 50% of the ZP thickness was removed in the thinning group, and a hole 40 µm in diameter was made in the drilling group...
September 2018: Clinical and Experimental Reproductive Medicine
Seok-Gi Kim, Youn-Young Kim, Ji-Young Park, Su-Jin Kwak, Chang-Seok Yoo, Il-Hae Park, Hong-Gil Sun, Jae-Won Kim, Kyeong-Ho Lee, Hum-Dai Park, Hee-Jun Chi
Objective: To determine whether fragment removal on in vitro fertilization (IVF) day 2 improved the subsequent development and pregnancy outcomes of fragmented embryos compared to similar-grade embryos without fragment removal. Methods: This study was a retrospective analysis involving 191 IVF cycles in which all embryos had over 10% fragmentation (grade 3 or 4) on day 2 of the IVF-embryo transfer cycle from March 2015 to December 2017. IVF cycles were divided into the fragment removal group (n=87) and the no fragment removal group (n=104) as a control cohort...
September 2018: Clinical and Experimental Reproductive Medicine
Sibel Sak, Hacer Uyanikoglu, Adnan Incebiyik, Hatice Incebiyik, Nese Gul Hilali, Tevfik Sabuncu, Erdal Sak
Objective: The aim of this study was to compare serum fetuin-A levels and oxidative stress markers, as indicators of insulin resistance, in women with polycystic ovary syndrome (PCOS) and in healthy controls. Methods: This prospective case-control study included 46 patients with PCOS and 48 age- and body mass index-matched control women. Levels of serum hormones, fetuin-A, and oxidative stress markers were measured in blood samples taken during the early follicular period from each participant...
September 2018: Clinical and Experimental Reproductive Medicine
Namfon Inna, Usanee Sanmee, Ubol Saeng-Anan, Waraporn Piromlertamorn, Teraporn Vutyavanich
Objective: To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. Methods: Two-cell mouse embryos were randomly allocated into three groups: a non-frozen control group (group 1, n=300), a group that underwent Cryotop vitrification (group 2, n=300), and a group that underwent our in-house freezing method (group 3, n=300). Results: There were no significant differences between groups 2 and 3 in the immediate survival rate (96...
September 2018: Clinical and Experimental Reproductive Medicine
Gi Young Kim
In an age when a small quantity of sperm can lead to pregnancy through in vitro fertilization or intracytoplasmic sperm injection, selecting healthy sperm is important. Sperm DNA fragmentation (SDF) is known to be higher in infertile men. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) and the alkaline comet test are SDF tests that directly measure DNA damage and have shown closer correlations with assisted reproduction results than indirect tools such as the sperm chromatin structure assay or the sperm chromatic dispersion test...
September 2018: Clinical and Experimental Reproductive Medicine
Hacer Uyanikoglu, Nese Gul Hilali, Mesut Yardimciel, Ismail Koyuncu
Objective: Prompt diagnosis and management are essential for saving the adnexal organs from infarction in cases of ovarian torsion (OT). This study aimed to determine the diagnostic significance of signal peptide, complement C1r/C1s, Uegf, and Bmp1 (CUB), and epidermal growth factor-like domain-containing protein-1 (SCUBE-1) levels in cases of OT, an emergent ischemic condition, and the relationship of SCUBE-1 with oxidative stress parameters. Methods: This prospective study was conducted among 15 OT patients and 20 age- and gravidity-matched healthy women...
June 2018: Clinical and Experimental Reproductive Medicine
Vidyalakshmi Subramanian, Aishwarya Ravichandran, Nivethitha Thiagarajan, Matheswari Govindarajan, Silambuchelvi Dhandayuthapani, Sujatha Suresh
Objective: The aim of this study was to measure reactive oxygen species (ROS) production and total antioxidant capacity (TAC) in the seminal fluid of the male partners in couples undergoing intrauterine insemination and to evaluate correlations between these values and their semen parameters. Methods: The study was conducted at Vamsam Fertility Center, Coimbatore, India and enrolled 110 male patients from whom semen samples were collected. ROS production was measured by a thiobarbituric acid reactive species assay, and TAC was measured by a 2,2-diphenyl-2-picrylhydrazyl free radical assay...
June 2018: Clinical and Experimental Reproductive Medicine
Hamid Ahmadi, Fardin Fathi, Ashraf Moeini, Fardin Amidi, Aligholi Sobhani
Objective: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring. Methods: Mice (6-8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size...
June 2018: Clinical and Experimental Reproductive Medicine
Dmab Dissanayake, H Patel, P S Wijesinghe
Objective: Recapitulation of the spermatogenesis process in vitro is a tool for studying the biology of germ cells, and may lead to promising therapeutic strategies in the future. In this study, we attempted to transdifferentiate Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into male germ cells using all- trans retinoic acid and Sertoli cell-conditioned medium. Methods: Human WJ-MSCs were propagated by the explant culture method, and cells at the second passage were induced with differentiation medium containing all- trans retinoic acid for 2 weeks...
June 2018: Clinical and Experimental Reproductive Medicine
Ayman Shehata Dawood, Hesham Abdelaziz Salem
The purpose of this paper is to review the current clinical uses of platelet-rich plasma (PRP) in the field of gynecology. All relevant articles published from January 2000 to December 2017 were reviewed and analyzed. The articles on PRP in the field of gynecology were mainly case series, pilot studies, or case reports. PRP is currently considered a new therapeutic modality for some disorders that are refractory to conventional drugs.
June 2018: Clinical and Experimental Reproductive Medicine
Ahmed T Alahmar
It has been estimated that approximately 15% of reproductive-age couples suffer from infertility. Male factors contribute to almost half of infertility cases, and in many patients the underlying cause of oligoasthenoteratozoospermia is unknown. Accumulating evidence suggests that oxidative stress plays a role as a contributing factor to male infertility, and reactive oxygen species have been shown to impair sperm function and motility and to damage sperm membrane and DNA. Therefore, this review explored the evidence provided by studies published from 2002 to 2017 on the impact of oral antioxidants (vitamin C, vitamin E, L-carnitine, coenzyme Q10, zinc, selenium, and pentoxifylline) on seminal fluid parameters in men with idiopathic oligoasthenoteratozoospermia...
June 2018: Clinical and Experimental Reproductive Medicine
(no author information available yet)
No abstract text is available yet for this article.
March 2018: Clinical and Experimental Reproductive Medicine
Yong Soo Hur, Eun Kyung Ryu, Chang Seop Hyun, Seong Ho Yang, San Hyun Yoon, Kyung Sil Lim, Won Don Lee, Jin Ho Lim
This study retrospectively assessed whether time-lapse data relating to developmental timing and morphology were associated with clinical outcomes, with the eventual goal of using morphokinetic variables to select embryos prospectively for cryopreservation. In this study, we examined the clinical outcomes of single vitrified-warmed blastocyst transfer cycles that were cultured in a time-lapse incubation system. The morphokinetic variables included uneven pronuclei, an uneven blastomere, multinucleation, and direct, rapid, and irregular division...
March 2018: Clinical and Experimental Reproductive Medicine
Shinnosuke Kuroda, Kimitsugu Usui, Kohei Mori, Kengo Yasuda, Takuo Asai, Hiroyuki Sanjo, Hiroyuki Yakanaka, Teppei Takeshima, Takashi Kawahara, Haruka Hamanoue, Yoshitake Kato, Yasuhide Miyoshi, Hiroji Uemura, Akira Iwasaki, Yasushi Yumura
We report the case of a 46-year-old Chinese male patient who visited our clinic complaining of infertility. Semen analysis revealed azoospermia, and azoospermia factor c region partial deletion (b1/b3) was detected using Y chromosome microdeletion analysis. Testicular sperm extraction was performed after genetic counseling. The bilateral ductus deferens and a portion of the epididymis were absent, whereas the remaining epididymis was expanded. Motile intratesticular spermatozoa were successfully extracted from the seminiferous tubule...
March 2018: Clinical and Experimental Reproductive Medicine
Shinnosuke Kuroda, Takuya Kondo, Kohei Mori, Kengo Yasuda, Takuo Asai, Hiroyuki Sanjo, Hiroyuki Yakanaka, Teppei Takeshima, Takashi Kawahara, Yoshitake Kato, Yasuhide Miyoshi, Hiroji Uemura, Akira Iwasaki, Yasushi Yumura
Onco-testicular sperm extraction is used to preserve fertility in patients with bilateral testicular tumors and azoospermia. We report the case of a testicular tumor in the solitary testis of a patient who had previously undergone successful contralateral orchiectomy and whose sperm was preserved by onco-testicular sperm extraction. A 35-year-old patient presented with swelling of his right scrotum that had lasted for 1 month. His medical history included a contralateral orchiectomy during childhood. Ultrasonography revealed a mosaic echoic area in his scrotum, suggesting a testicular tumor...
March 2018: Clinical and Experimental Reproductive Medicine
Sarapan Na Nakhon, Pawan Limvorapitux, Patsama Vichinsartvichai
Objective: To survey knowledge about the factors that influence fertility in a reproductive-age population living in an urban area. Methods: A cross-sectional study was conducted using a questionnaire-based survey among both males and females aged 18-45 years living in the Bangkok metropolitan area. Results: The mean age of the participants was 26.8±7.2 years (male, 41.9%; female, 58.1%). Of the participants, 53.1% had an undergraduate degree and 57...
March 2018: Clinical and Experimental Reproductive Medicine
Hwa Seon Koo, In Ok Song, Sun Hwa Cha, Chan Woo Park, Hye Ok Kim
Objective: To evaluate the pregnancy rate and time to pregnancy after timed coitus with or without superovulation in infertile young women younger than 35 years old with low serum anti-Müllerian hormone (AMH) levels (<25th percentile). Methods: A total of 202 patients younger than 35 years old were recruited retrospectively between 2010 and 2012. Ninety-eight women had normal serum AMH levels (25-75th percentile), 75 women had low serum AMH levels (5th≤&<25th percentile) and 29 women had very low serum AMH levels (<5th percentile), according to reference values for their age group...
March 2018: Clinical and Experimental Reproductive Medicine
Ju-Eun Jeong, Bo-Sun Joo, Chang-Woon Kim, Hwi-Gon Kim, Jong-Kil Joo, Kyu-Sup Lee
Objective: This study conducted a preliminary examination of the effects of three-area laser-assisted zona thinning (LAZT) during the cleavage stage of embryo development on the hatching process in human in vitro fertilization-embryo transfer (IVF-ET) with subjects of advanced female age or frozen-thawed (FT) embryos. Methods: Eight-cell stage embryos were treated with LAZT in three areas of the zona pellucida at 120° intervals. The control group was embryos without LAZT...
March 2018: Clinical and Experimental Reproductive Medicine
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