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Clinical and Experimental Reproductive Medicine

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https://www.readbyqxmd.com/read/29662828/acknowledgment-to-reviewers-for-2017
#1
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March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662827/retrospective-study-of-single-vitrified-warmed-blastocyst-transfer-cycles-according-to-the-presence-of-morphokinetic-variables
#2
Yong Soo Hur, Eun Kyung Ryu, Chang Seop Hyun, Seong Ho Yang, San Hyun Yoon, Kyung Sil Lim, Won Don Lee, Jin Ho Lim
This study retrospectively assessed whether time-lapse data relating to developmental timing and morphology were associated with clinical outcomes, with the eventual goal of using morphokinetic variables to select embryos prospectively for cryopreservation. In this study, we examined the clinical outcomes of single vitrified-warmed blastocyst transfer cycles that were cultured in a time-lapse incubation system. The morphokinetic variables included uneven pronuclei, an uneven blastomere, multinucleation, and direct, rapid, and irregular division...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662826/an-infertile-patient-with-y-chromosome-b1-b3-deletion-presenting-with-congenital-bilateral-absence-of-the-vas-deferens-with-normal-spermatogenesis
#3
Shinnosuke Kuroda, Kimitsugu Usui, Kohei Mori, Kengo Yasuda, Takuo Asai, Hiroyuki Sanjo, Hiroyuki Yakanaka, Teppei Takeshima, Takashi Kawahara, Haruka Hamanoue, Yoshitake Kato, Yasuhide Miyoshi, Hiroji Uemura, Akira Iwasaki, Yasushi Yumura
We report the case of a 46-year-old Chinese male patient who visited our clinic complaining of infertility. Semen analysis revealed azoospermia, and azoospermia factor c region partial deletion (b1/b3) was detected using Y chromosome microdeletion analysis. Testicular sperm extraction was performed after genetic counseling. The bilateral ductus deferens and a portion of the epididymis were absent, whereas the remaining epididymis was expanded. Motile intratesticular spermatozoa were successfully extracted from the seminiferous tubule...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662825/successful-onco-testicular-sperm-extraction-from-a-testicular-cancer-patient-with-a-single-testis-and-azoospermia
#4
Shinnosuke Kuroda, Takuya Kondo, Kohei Mori, Kengo Yasuda, Takuo Asai, Hiroyuki Sanjo, Hiroyuki Yakanaka, Teppei Takeshima, Takashi Kawahara, Yoshitake Kato, Yasuhide Miyoshi, Hiroji Uemura, Akira Iwasaki, Yasushi Yumura
Onco-testicular sperm extraction is used to preserve fertility in patients with bilateral testicular tumors and azoospermia. We report the case of a testicular tumor in the solitary testis of a patient who had previously undergone successful contralateral orchiectomy and whose sperm was preserved by onco-testicular sperm extraction. A 35-year-old patient presented with swelling of his right scrotum that had lasted for 1 month. His medical history included a contralateral orchiectomy during childhood. Ultrasonography revealed a mosaic echoic area in his scrotum, suggesting a testicular tumor...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662824/knowledge-regarding-factors-that-influence-fertility-in-thai-reproductive-age-population-living-in-urban-area-a-cross-sectional-study
#5
Sarapan Na Nakhon, Pawan Limvorapitux, Patsama Vichinsartvichai
Objective: To survey knowledge about the factors that influence fertility in a reproductive-age population living in an urban area. Methods: A cross-sectional study was conducted using a questionnaire-based survey among both males and females aged 18-45 years living in the Bangkok metropolitan area. Results: The mean age of the participants was 26.8±7.2 years (male, 41.9%; female, 58.1%). Of the participants, 53.1% had an undergraduate degree and 57...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662823/the-likelihood-of-achieving-pregnancy-through-timed-coitus-in-young-infertile-women-with-decreased-ovarian-reserve
#6
Hwa Seon Koo, In Ok Song, Sun Hwa Cha, Chan Woo Park, Hye Ok Kim
Objective: To evaluate the pregnancy rate and time to pregnancy after timed coitus with or without superovulation in infertile young women younger than 35 years old with low serum anti-Müllerian hormone (AMH) levels (<25th percentile). Methods: A total of 202 patients younger than 35 years old were recruited retrospectively between 2010 and 2012. Ninety-eight women had normal serum AMH levels (25-75th percentile), 75 women had low serum AMH levels (5th≤&<25th percentile) and 29 women had very low serum AMH levels (<5th percentile), according to reference values for their age group...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662822/effects-of-three-area-laser-assisted-zona-thinning-in-8-cell-human-embryos-on-pregnancy-outcomes-in-vitro-fertilization
#7
Ju-Eun Jeong, Bo-Sun Joo, Chang-Woon Kim, Hwi-Gon Kim, Jong-Kil Joo, Kyu-Sup Lee
Objective: This study conducted a preliminary examination of the effects of three-area laser-assisted zona thinning (LAZT) during the cleavage stage of embryo development on the hatching process in human in vitro fertilization-embryo transfer (IVF-ET) with subjects of advanced female age or frozen-thawed (FT) embryos. Methods: Eight-cell stage embryos were treated with LAZT in three areas of the zona pellucida at 120° intervals. The control group was embryos without LAZT...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662821/sperm-chromatin-and-dna-integrity-methyltransferase-mrna-levels-and-global-dna-methylation-in-oligoasthenoteratozoospermia
#8
Tahereh Rahiminia, Ehsan Farashahi Yazd, Farzaneh Fesahat, Mohammad Reza Moein, Ali Mohammad Mirjalili, Ali Reza Talebi
Objective: To investigate sperm chromatin/DNA integrity, global DNA methylation, and DNMT mRNA transcription in men with oligoasthenoteratozoospermia (OAT) compared with normozoospermic men. Methods: Semen samples from 32 OAT patients who comprised the case group and 32 normozoospermic men who comprised the control group were isolated and purified using a standard gradient isolation procedure according to World Health Organization criteria. DNMT1 , DNMT3A , and DNMT3B transcripts were then compared between groups using real-time quantitative reverse-transcription polymerase chain reaction...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662820/effects-of-selenium-on-the-survival-and-invasion-of-trophoblasts
#9
Jee Yoon Na, Jin Seok, Sohae Park, Jung Seok Kim, Gi Jin Kim
Objective: Placental oxidative stress is known to be a factor that contributes to pregnancy failure. The aim of this study was to determine whether selenium could induce antioxidant gene expression and regulate invasive activity and mitochondrial activity in trophoblasts, which are a major cell type of the placenta. Methods: To understand the effects of selenium on trophoblast cells exposed to hypoxia, the viability and invasive activity of trophoblasts were analyzed...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662819/variable-localization-of-toll-like-receptors-in-human-fallopian-tube-epithelial-cells
#10
Fatemehsadat Amjadi, Zahra Zandieh, Ensieh Salehi, Reza Jafari, Nasrin Ghasemi, Abbas Aflatoonian, Alireza Fazeli, Reza Aflatoonian
Objective: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. Methods: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376021/reduction-of-the-cetrorelix-dose-in-a-multiple-dose-antagonist-protocol-and-its-impact-on-pregnancy-rate-and-affordability-a-randomized-controlled-multicenter-study
#11
Ayman S Dawood, Adel Algergawy, Ahmed Elhalwagy
Objective: To determine whether reducing the cetrorelix dose in the antagonist protocol to 0.125 mg had any deleterious effects on follicular development, the number and quality of retrieved oocytes, or the number of embryos, and to characterize its effects on the affordability of assisted reproductive technology. Methods: This randomized controlled study was conducted at the Fertility Unit of Tanta Educational Hospital of Tanta University, the Egyptian Consultants' Fertility Center, and the Qurrat Aien Fertility Center, from January 1 to June 30, 2017...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376020/impact-of-sperm-dna-fragmentation-on-clinical-in-vitro-fertilization-outcomes
#12
Hwa Young Choi, Seul Ki Kim, Seok Hyun Kim, Young Min Choi, Byung Chul Jee
Objective: We studied the association between sperm DNA fragmentation (SDF) and several clinical in vitro fertilization outcomes. Methods: We retrospectively analyzed 169 consecutive fresh IVF cycles. Semen was collected on the day of oocyte retrieval, and we assessed standard semen parameters and the SDF level (by terminal deoxynucleotidyl transferase dUTP nick-end labeling). Poor ovarian response (POR) was defined as the collection of three or fewer mature oocytes...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376019/t-helper-cell-subsets-and-related-cytokines-in-infertile-women-undergoing-in-vitro-fertilization-before-and-after-seminal-plasma-exposure
#13
Marziyeh Azad, Sara Keshtgar, Bahia Namavar Jahromi, Zahra Kanannejad, Behrouz Gharesi-Fard
Objective: In vitro fertilization (IVF) is a well-known method for the treatment of infertility. The present study aimed to compare the differences between infertile women with successful and unsuccessful IVF outcomes regarding the expression of T helper (Th) cell transcription factors and a group of related cytokines before and after exposure to their husbands' seminal plasma. Methods: This study was performed on 19 couples with unexplained infertility undergoing IVF treatment...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376018/effects-of-infections-with-five-sexually-transmitted-pathogens-on-sperm-quality
#14
Sung Jae Kim, Doo-Jin Paik, Joong Shik Lee, Hyo Serk Lee, Ju Tae Seo, Mi Seon Jeong, Jae-Ho Lee, Dong Wook Park, Sangchul Han, Yoo Kyung Lee, Ki Heon Lee, In Ho Lee, Kyeong A So, Seon Ah Kim, Juree Kim, Tae Jin Kim
Objective: This study investigated the prevalence of infections with human papillomavirus, Chlamydia trachomatis , Ureaplasma urealyticum , Mycoplasma hominis , and Mycoplasma genitalium in the semen of Korean infertile couples and their associations with sperm quality. Methods: Semen specimens were collected from 400 men who underwent a fertility evaluation. Infection with above five pathogens was assessed in each specimen. Sperm quality was compared in the pathogen-infected group and the non-infected group...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376017/sperm-dna-fragmentation-and-sex-chromosome-aneuploidy-after-swim-up-versus-density-gradient-centrifugation
#15
Sung Woo Kim, Byung Chul Jee, Seul Ki Kim, Seok Hyun Kim
Objective: The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. Methods: Semen samples were obtained from 18 healthy male partners who attended infertility clinics for infertility investigations and were processed with swim-up and DGC. The percentages of sperm cells with fragmented DNA measured by the sperm chromatin dispersion test, normal sex chromosomes assessed by fluorescence in situ hybridization, and abnormal chromatin structure identified by toluidine blue staining were examined...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376016/effects-of-laser-assisted-hatching-and-exposure-time-to-vitrification-solution-on-mouse-embryo-development
#16
Hye Jin Kim, Sung Baek Park, Jung Bo Yang, Young Bae Choi, Ki Hwan Lee
Objective: This study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers. Methods: First, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinning-assisted hatching before vitrification (4, 6, and 8 minutes or 4, 7, and 10 minutes, respectively)...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376015/fertility-preservation-for-patients-with-hematologic-malignancies-the-korean-society-for-fertility-preservation-clinical-guidelines
#17
REVIEW
Dong-Yun Lee, Seul Ki Kim, Miran Kim, Kyung Joo Hwang, Seok Hyun Kim
Although the survival rate of hematologic malignancies in young patients is very high, cytotoxic therapies such as chemotherapy and total body irradiation therapy can significantly reduce a patient's reproductive capacity and cause irreversible infertility. Early ovarian failure also commonly occurs following additional cancer treatment, bone marrow transplantation, or autologous transplantation. Because the risk of early ovarian failure depends on the patient's circumstances, patients with a hematologic malignancy must consult health professionals regarding fertility preservation before undergoing treatments that can potentially damage their ovaries...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376014/fertility-preservation-for-patients-with-breast-cancer-the-korean-society-for-fertility-preservation-clinical-guidelines
#18
REVIEW
Hoon Kim, Seul Ki Kim, Jung Ryeol Lee, Kyung Joo Hwang, Chang Suk Suh, Seok Hyun Kim
With advances in the methods of cancer treatment used in modern medicine, the number of breast cancer survivors has been consistently rising. As the number of women who wish to become pregnant after being diagnosed with breast cancer increases, it is necessary to consider fertility preservation in these patients. However, medical doctors may be unaware of the importance of fertility preservation among cancer patients because most patients do not share their concerns about fertility with their doctors. Considering the time spent choosing and undergoing treatment, an early referral to a reproductive specialist is the best way to prevent a delay in cancer treatment...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376013/fertility-preservation-for-patients-with-gynecologic-malignancies-the-korean-society-for-fertility-preservation-clinical-guidelines
#19
REVIEW
Sanghoon Lee, Seul Ki Kim, Kyung Joo Hwang, Tak Kim, Seok Hyun Kim
Fertility preservation plays a central role in cancer care since an increasingly large number of cancer patients are surviving as a result of improvements in diagnostic and therapeutic strategies. Physicians who take part in the initial diagnosis and management of gynecologic cancer should understand the importance of fertility preservation. Since indications for fertility preservation are limited to early-stage gynecologic cancer, a surgeon must carefully consider each indication. Before performing fertility-sparing surgery, health professionals should compare its oncologic and pregnancy outcomes with those of other standard treatments...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376012/fertility-preservation-during-cancer-treatment-the-korean-society-for-fertility-preservation-clinical-guidelines
#20
REVIEW
Jayeon Kim, Seul Ki Kim, Kyung Joo Hwang, Seok Hyun Kim
While many fertility preservation (FP) options now exist for reproductive-aged cancer patients, access to these services continues to be limited. A comprehensive FP program should be organized to serve oncofertility patients effectively. Also, much effort is needed from various individuals-patients, specialists from various fields, and consultants-to facilitate FP in a timely manner. Various challenges still exist in improving access to FP programs. To improve access to FP treatment, it is important to educate oncologists and patients via electronic tools and to actively navigate patients through the system...
December 2017: Clinical and Experimental Reproductive Medicine
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