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Clinical and Experimental Reproductive Medicine

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https://www.readbyqxmd.com/read/29984210/a-new-biomarker-for-the-early-diagnosis-of-ovarian-torsion-scube-1
#1
Hacer Uyanikoglu, Nese Gul Hilali, Mesut Yardimciel, Ismail Koyuncu
Objective: Prompt diagnosis and management are essential for saving the adnexal organs from infarction in cases of ovarian torsion (OT). This study aimed to determine the diagnostic significance of signal peptide, complement C1r/C1s, Uegf, and Bmp1 (CUB), and epidermal growth factor-like domain-containing protein-1 (SCUBE-1) levels in cases of OT, an emergent ischemic condition, and the relationship of SCUBE-1 with oxidative stress parameters. Methods: This prospective study was conducted among 15 OT patients and 20 age- and gravidity-matched healthy women...
June 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29984209/seminal-reactive-oxygen-species-and-total-antioxidant-capacity-correlations-with-sperm-parameters-and-impact-on-male-infertility
#2
Vidyalakshmi Subramanian, Aishwarya Ravichandran, Nivethitha Thiagarajan, Matheswari Govindarajan, Silambuchelvi Dhandayuthapani, Sujatha Suresh
Objective: The aim of this study was to measure reactive oxygen species (ROS) production and total antioxidant capacity (TAC) in the seminal fluid of the male partners in couples undergoing intrauterine insemination and to evaluate correlations between these values and their semen parameters. Methods: The study was conducted at Vamsam Fertility Center, Coimbatore, India and enrolled 110 male patients from whom semen samples were collected. ROS production was measured by a thiobarbituric acid reactive species assay, and TAC was measured by a 2,2-diphenyl-2-picrylhydrazyl free radical assay...
June 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29984208/evaluation-of-prooxidant-antioxidant-balance-in-in-vitro-fertilization-conceived-mice
#3
Hamid Ahmadi, Fardin Fathi, Ashraf Moeini, Fardin Amidi, Aligholi Sobhani
Objective: Concerns about the safety of assisted reproductive technology (ART) have been raised, as some studies have shown elevated incidence rates of childhood cancer, asthma, allergies, and other diseases in ART-conceived babies. Findings regarding the health of ART-conceived babies are controversial. The present study was conducted to evaluate the prooxidant-antioxidant balance (PAB) in in vitro fertilization (IVF)-conceived mice in comparison to naturally conceived offspring. Methods: Mice (6-8 weeks) were divided into two groups (IVF-conceived and naturally conceived) matched by sex, age, weight, and litter size...
June 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29984207/differentiation-of-human-male-germ-cells-from-wharton-s-jelly-derived-mesenchymal-stem-cells
#4
Dmab Dissanayake, H Patel, P S Wijesinghe
Objective: Recapitulation of the spermatogenesis process in vitro is a tool for studying the biology of germ cells, and may lead to promising therapeutic strategies in the future. In this study, we attempted to transdifferentiate Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) into male germ cells using all- trans retinoic acid and Sertoli cell-conditioned medium. Methods: Human WJ-MSCs were propagated by the explant culture method, and cells at the second passage were induced with differentiation medium containing all- trans retinoic acid for 2 weeks...
June 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29984206/current-clinical-applications-of-platelet-rich-plasma-in-various-gynecological-disorders-an-appraisal-of-theory-and-practice
#5
REVIEW
Ayman Shehata Dawood, Hesham Abdelaziz Salem
The purpose of this paper is to review the current clinical uses of platelet-rich plasma (PRP) in the field of gynecology. All relevant articles published from January 2000 to December 2017 were reviewed and analyzed. The articles on PRP in the field of gynecology were mainly case series, pilot studies, or case reports. PRP is currently considered a new therapeutic modality for some disorders that are refractory to conventional drugs.
June 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29984205/the-effects-of-oral-antioxidants-on-the-semen-of-men-with-idiopathic-oligoasthenoteratozoospermia
#6
REVIEW
Ahmed T Alahmar
It has been estimated that approximately 15% of reproductive-age couples suffer from infertility. Male factors contribute to almost half of infertility cases, and in many patients the underlying cause of oligoasthenoteratozoospermia is unknown. Accumulating evidence suggests that oxidative stress plays a role as a contributing factor to male infertility, and reactive oxygen species have been shown to impair sperm function and motility and to damage sperm membrane and DNA. Therefore, this review explored the evidence provided by studies published from 2002 to 2017 on the impact of oral antioxidants (vitamin C, vitamin E, L-carnitine, coenzyme Q10, zinc, selenium, and pentoxifylline) on seminal fluid parameters in men with idiopathic oligoasthenoteratozoospermia...
June 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662828/acknowledgment-to-reviewers-for-2017
#7
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662827/retrospective-study-of-single-vitrified-warmed-blastocyst-transfer-cycles-according-to-the-presence-of-morphokinetic-variables
#8
Yong Soo Hur, Eun Kyung Ryu, Chang Seop Hyun, Seong Ho Yang, San Hyun Yoon, Kyung Sil Lim, Won Don Lee, Jin Ho Lim
This study retrospectively assessed whether time-lapse data relating to developmental timing and morphology were associated with clinical outcomes, with the eventual goal of using morphokinetic variables to select embryos prospectively for cryopreservation. In this study, we examined the clinical outcomes of single vitrified-warmed blastocyst transfer cycles that were cultured in a time-lapse incubation system. The morphokinetic variables included uneven pronuclei, an uneven blastomere, multinucleation, and direct, rapid, and irregular division...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662826/an-infertile-patient-with-y-chromosome-b1-b3-deletion-presenting-with-congenital-bilateral-absence-of-the-vas-deferens-with-normal-spermatogenesis
#9
Shinnosuke Kuroda, Kimitsugu Usui, Kohei Mori, Kengo Yasuda, Takuo Asai, Hiroyuki Sanjo, Hiroyuki Yakanaka, Teppei Takeshima, Takashi Kawahara, Haruka Hamanoue, Yoshitake Kato, Yasuhide Miyoshi, Hiroji Uemura, Akira Iwasaki, Yasushi Yumura
We report the case of a 46-year-old Chinese male patient who visited our clinic complaining of infertility. Semen analysis revealed azoospermia, and azoospermia factor c region partial deletion (b1/b3) was detected using Y chromosome microdeletion analysis. Testicular sperm extraction was performed after genetic counseling. The bilateral ductus deferens and a portion of the epididymis were absent, whereas the remaining epididymis was expanded. Motile intratesticular spermatozoa were successfully extracted from the seminiferous tubule...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662825/successful-onco-testicular-sperm-extraction-from-a-testicular-cancer-patient-with-a-single-testis-and-azoospermia
#10
Shinnosuke Kuroda, Takuya Kondo, Kohei Mori, Kengo Yasuda, Takuo Asai, Hiroyuki Sanjo, Hiroyuki Yakanaka, Teppei Takeshima, Takashi Kawahara, Yoshitake Kato, Yasuhide Miyoshi, Hiroji Uemura, Akira Iwasaki, Yasushi Yumura
Onco-testicular sperm extraction is used to preserve fertility in patients with bilateral testicular tumors and azoospermia. We report the case of a testicular tumor in the solitary testis of a patient who had previously undergone successful contralateral orchiectomy and whose sperm was preserved by onco-testicular sperm extraction. A 35-year-old patient presented with swelling of his right scrotum that had lasted for 1 month. His medical history included a contralateral orchiectomy during childhood. Ultrasonography revealed a mosaic echoic area in his scrotum, suggesting a testicular tumor...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662824/knowledge-regarding-factors-that-influence-fertility-in-thai-reproductive-age-population-living-in-urban-area-a-cross-sectional-study
#11
Sarapan Na Nakhon, Pawan Limvorapitux, Patsama Vichinsartvichai
Objective: To survey knowledge about the factors that influence fertility in a reproductive-age population living in an urban area. Methods: A cross-sectional study was conducted using a questionnaire-based survey among both males and females aged 18-45 years living in the Bangkok metropolitan area. Results: The mean age of the participants was 26.8±7.2 years (male, 41.9%; female, 58.1%). Of the participants, 53.1% had an undergraduate degree and 57...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662823/the-likelihood-of-achieving-pregnancy-through-timed-coitus-in-young-infertile-women-with-decreased-ovarian-reserve
#12
Hwa Seon Koo, In Ok Song, Sun Hwa Cha, Chan Woo Park, Hye Ok Kim
Objective: To evaluate the pregnancy rate and time to pregnancy after timed coitus with or without superovulation in infertile young women younger than 35 years old with low serum anti-Müllerian hormone (AMH) levels (<25th percentile). Methods: A total of 202 patients younger than 35 years old were recruited retrospectively between 2010 and 2012. Ninety-eight women had normal serum AMH levels (25-75th percentile), 75 women had low serum AMH levels (5th≤&<25th percentile) and 29 women had very low serum AMH levels (<5th percentile), according to reference values for their age group...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662822/effects-of-three-area-laser-assisted-zona-thinning-in-8-cell-human-embryos-on-pregnancy-outcomes-in-vitro-fertilization
#13
Ju-Eun Jeong, Bo-Sun Joo, Chang-Woon Kim, Hwi-Gon Kim, Jong-Kil Joo, Kyu-Sup Lee
Objective: This study conducted a preliminary examination of the effects of three-area laser-assisted zona thinning (LAZT) during the cleavage stage of embryo development on the hatching process in human in vitro fertilization-embryo transfer (IVF-ET) with subjects of advanced female age or frozen-thawed (FT) embryos. Methods: Eight-cell stage embryos were treated with LAZT in three areas of the zona pellucida at 120° intervals. The control group was embryos without LAZT...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662821/sperm-chromatin-and-dna-integrity-methyltransferase-mrna-levels-and-global-dna-methylation-in-oligoasthenoteratozoospermia
#14
Tahereh Rahiminia, Ehsan Farashahi Yazd, Farzaneh Fesahat, Mohammad Reza Moein, Ali Mohammad Mirjalili, Ali Reza Talebi
Objective: To investigate sperm chromatin/DNA integrity, global DNA methylation, and DNMT mRNA transcription in men with oligoasthenoteratozoospermia (OAT) compared with normozoospermic men. Methods: Semen samples from 32 OAT patients who comprised the case group and 32 normozoospermic men who comprised the control group were isolated and purified using a standard gradient isolation procedure according to World Health Organization criteria. DNMT1 , DNMT3A , and DNMT3B transcripts were then compared between groups using real-time quantitative reverse-transcription polymerase chain reaction...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662820/effects-of-selenium-on-the-survival-and-invasion-of-trophoblasts
#15
Jee Yoon Na, Jin Seok, Sohae Park, Jung Seok Kim, Gi Jin Kim
Objective: Placental oxidative stress is known to be a factor that contributes to pregnancy failure. The aim of this study was to determine whether selenium could induce antioxidant gene expression and regulate invasive activity and mitochondrial activity in trophoblasts, which are a major cell type of the placenta. Methods: To understand the effects of selenium on trophoblast cells exposed to hypoxia, the viability and invasive activity of trophoblasts were analyzed...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662819/variable-localization-of-toll-like-receptors-in-human-fallopian-tube-epithelial-cells
#16
Fatemehsadat Amjadi, Zahra Zandieh, Ensieh Salehi, Reza Jafari, Nasrin Ghasemi, Abbas Aflatoonian, Alireza Fazeli, Reza Aflatoonian
Objective: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. Methods: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376021/reduction-of-the-cetrorelix-dose-in-a-multiple-dose-antagonist-protocol-and-its-impact-on-pregnancy-rate-and-affordability-a-randomized-controlled-multicenter-study
#17
Ayman S Dawood, Adel Algergawy, Ahmed Elhalwagy
Objective: To determine whether reducing the cetrorelix dose in the antagonist protocol to 0.125 mg had any deleterious effects on follicular development, the number and quality of retrieved oocytes, or the number of embryos, and to characterize its effects on the affordability of assisted reproductive technology. Methods: This randomized controlled study was conducted at the Fertility Unit of Tanta Educational Hospital of Tanta University, the Egyptian Consultants' Fertility Center, and the Qurrat Aien Fertility Center, from January 1 to June 30, 2017...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376020/impact-of-sperm-dna-fragmentation-on-clinical-in-vitro-fertilization-outcomes
#18
Hwa Young Choi, Seul Ki Kim, Seok Hyun Kim, Young Min Choi, Byung Chul Jee
Objective: We studied the association between sperm DNA fragmentation (SDF) and several clinical in vitro fertilization outcomes. Methods: We retrospectively analyzed 169 consecutive fresh IVF cycles. Semen was collected on the day of oocyte retrieval, and we assessed standard semen parameters and the SDF level (by terminal deoxynucleotidyl transferase dUTP nick-end labeling). Poor ovarian response (POR) was defined as the collection of three or fewer mature oocytes...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376019/t-helper-cell-subsets-and-related-cytokines-in-infertile-women-undergoing-in-vitro-fertilization-before-and-after-seminal-plasma-exposure
#19
Marziyeh Azad, Sara Keshtgar, Bahia Namavar Jahromi, Zahra Kanannejad, Behrouz Gharesi-Fard
Objective: In vitro fertilization (IVF) is a well-known method for the treatment of infertility. The present study aimed to compare the differences between infertile women with successful and unsuccessful IVF outcomes regarding the expression of T helper (Th) cell transcription factors and a group of related cytokines before and after exposure to their husbands' seminal plasma. Methods: This study was performed on 19 couples with unexplained infertility undergoing IVF treatment...
December 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29376018/effects-of-infections-with-five-sexually-transmitted-pathogens-on-sperm-quality
#20
Sung Jae Kim, Doo-Jin Paik, Joong Shik Lee, Hyo Serk Lee, Ju Tae Seo, Mi Seon Jeong, Jae-Ho Lee, Dong Wook Park, Sangchul Han, Yoo Kyung Lee, Ki Heon Lee, In Ho Lee, Kyeong A So, Seon Ah Kim, Juree Kim, Tae Jin Kim
Objective: This study investigated the prevalence of infections with human papillomavirus, Chlamydia trachomatis , Ureaplasma urealyticum , Mycoplasma hominis , and Mycoplasma genitalium in the semen of Korean infertile couples and their associations with sperm quality. Methods: Semen specimens were collected from 400 men who underwent a fertility evaluation. Infection with above five pathogens was assessed in each specimen. Sperm quality was compared in the pathogen-infected group and the non-infected group...
December 2017: Clinical and Experimental Reproductive Medicine
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