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Clinical and Experimental Reproductive Medicine

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https://www.readbyqxmd.com/read/28090465/does-intrauterine-injection-of-low-molecular-weight-heparin-improve-the-clinical-pregnancy-rate-in-intracytoplasmic-sperm-injection
#1
Ahmed Mohamed Kamel, Yahia El-Faissal, Mona Aboulghar, Ragaa Mansour, Gamal I Serour, Mohamed Aboulghar
OBJECTIVE: Heparin can modulate proteins, and influence processes involved in implantation and trophoblastic development. This study aimed to assess the improvement of clinical pregnancy and implantation rates after local intrauterine injection of low-molecular-weight heparin (LMWH) in patients undergoing intracytoplasmic sperm injection (ICSI). METHODS: A randomised case/control design was followed in women scheduled for ICSI. The study arm was injected with intrauterine LMWH during mock embryo transfer immediately following the ovum pickup procedure, while the control arm was given an intrauterine injection with a similar volume of tissue culture media...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090464/the-efficacy-of-intrauterine-instillation-of-granulocyte-colony-stimulating-factor-in-infertile-women-with-a-thin-endometrium-a-pilot-study
#2
Dayong Lee, Jae Dong Jo, Seul Ki Kim, Byung Chul Jee, Seok Hyun Kim
OBJECTIVE: The study aimed to investigate the efficacy of intrauterine instillation of granulocyte colony-stimulating factor (G-CSF) on the day of ovulation triggering or oocyte retrieval in infertile women with a thin endometrium. METHODS: Fifty women whose endometrial thickness (EMT) was ≤8 mm at the time of triggering during at least one previous in vitro fertilization (IVF) cycle and an index IVF cycle were selected. On the day of triggering (n=12) or oocyte retrieval (n=38), 300 µg of G-CSF was instilled into the uterine cavity...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090463/efficacy-of-oxytocin-antagonist-infusion-in-improving-in-vitro-fertilization-outcomes-on-the-day-of-embryo-transfer-a-meta-analysis
#3
Seul Ki Kim, E-Jung Han, Sun Mie Kim, Jung Ryeol Lee, Byung Chul Jee, Chang Suk Suh, Seok Hyun Kim
OBJECTIVE: Uterine contraction induced by the embryo transfer (ET) process has an adverse effect on embryo implantation. The aim of this study was to determine the effect of oxytocin antagonist supplementation on the day of ET on in vitro fertilization outcomes via a meta-analysis. METHODS: We performed a meta-analysis of randomized controlled trials (RCTs). Four online databases (Embase, Medline, PubMed, and Cochrane Library) were searched through May 2015 for RCTs that investigated oxytocin antagonist supplementation on the day of ET...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090462/pretreatment-of-normal-responders-in-fresh-in-vitro-fertilization-cycles-a-comparison-of-transdermal-estradiol-and-oral-contraceptive-pills
#4
Nigel Pereira, Allison C Petrini, Zhen N Zhou, Jovana P Lekovich, Isaac Kligman, Zev Rosenwaks
OBJECTIVE: The aim of this study was to investigate the impact of pretreatment with transdermal estradiol (E2) compared to oral contraceptive pills (OCPs) on controlled ovarian stimulation (COS) response in normal responders undergoing fresh in vitro fertilization (IVF)-embryo transfer (ET) cycles. METHODS: A retrospective cohort study was performed of normal responders undergoing fresh IVF-ET cycles who received pretreatment with transdermal E2 versus OCPs prior to fresh IVF-ET...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090461/effects-of-maternal-age-on-embryo-quality-and-pregnancy-outcomes-using-testicular-sperm-with-intracytoplasmic-sperm-injection
#5
Hye Won Choi, Yong-Seog Park, Sun-Hee Lee, Chun Kyu Lim, Ju Tae Seo, Kwang Moon Yang
OBJECTIVE: The aim of this study was to evaluate the influence of maternal age on fertilization, embryo quality, and clinical pregnancy in patients undergoing intracytoplasmic sperm injection (ICSI) using testicular sperm from partners with azoospermia. METHODS: A total of 416 ICSI cycles using testicular spermatozoa from partners with obstructive azoospermia (OA, n=301) and non-obstructive azoospermia (NOA, n=115) were analyzed. Female patients were divided into the following age groups: 27 to 31 years, 32 to 36 years, and 37 to 41 years...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090460/efficacy-and-safety-of-dienogest-in-patients-with-endometriosis-a-single-center-observational-study-over-12-months
#6
So Yun Park, Sung Hoon Kim, Hee Dong Chae, Chung-Hoon Kim, Byung Moon Kang
OBJECTIVE: To evaluate the efficacy and safety of dienogest treatment in patients who had received dienogest for 12 months or more to treat endometriosis. METHODS: We analyzed the clinical data of 188 women with endometriosis who had been treated with 2 mg of dienogest once a day for 12 months or more at a single institute. We evaluated changes in endometriosis-associated pain and endometrioma size, recurrence rate, and adverse events following dienogest administration...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090459/anti-m%C3%A3-llerian-hormone-as-a-predictor-of-polycystic-ovary-syndrome-treated-with-clomiphene-citrate
#7
Andon Hestiantoro, Yuwono Sri Negoro, Yohana Afrita, Budi Wiweko, Kanadi Sumapradja, Muharam Natadisastra
OBJECTIVE: This study aimed to determine the threshold of anti-Müllerian hormone (AMH) as predictor of follicular growth failure in polycystic ovary syndrome (PCOS) patients treated with clomiphene citrate (CC). METHODS: Fifty female subjects with PCOS were recruited and divided into two groups based on successful and unsuccessful follicular growth. Related variables such as age, infertility duration, cigarette smoking, use of Moslem hijab, sunlight exposure, fiber intake, body mass index, waist circumference, AMH level, 25-hydroxy vitamin D level, and growth of dominant follicles were obtained, assessed, and statistically analyzed...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090458/efficient-isolation-of-sperm-with-high-dna-integrity-and-stable-chromatin-packaging-by-a-combination-of-density-gradient-centrifugation-and-magnetic-activated-cell-sorting
#8
Hee-Jun Chi, Su-Jin Kwak, Seok-Gi Kim, Youn-Young Kim, Ji-Young Park, Chang-Seok Yoo, Il-Hae Park, Hong-Gil Sun, Jae-Won Kim, Kyeong-Ho Lee
OBJECTIVE: This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. METHODS: Semen analysis and a sperm DNA fragmentation assay were performed to assess the correlations between semen parameters and the DFI in 458 semen samples...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090457/glucose-6-phosphate-dehydrogenase-deficiency-does-not-increase-the-susceptibility-of-sperm-to-oxidative-stress-induced-by-h2o2
#9
Shiva Roshankhah, Zahra Rostami-Far, Farhad Shaveisi-Zadeh, Abolfazl Movafagh, Mitra Bakhtiari, Jila Shaveisi-Zadeh
OBJECTIVE: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect. G6PD plays a key role in the pentose phosphate pathway, which is a major source of nicotinamide adenine dinucleotide phosphate (NADPH). NADPH provides the reducing equivalents for oxidation-reduction reductions involved in protecting against the toxicity of reactive oxygen species such as H2O2. We hypothesized that G6PD deficiency may reduce the amount of NADPH in sperms, thereby inhibiting the detoxification of H2O2, which could potentially affect their motility and viability, resulting in an increased susceptibility to infertility...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28090456/chronic-endometritis-and-infertility
#10
REVIEW
Hyun Jong Park, You Shin Kim, Tae Ki Yoon, Woo Sik Lee
Chronic endometritis (CE) is a condition involving the breakdown of the peaceful co-existence between microorganisms and the host immune system in the endometrium. A majority of CE cases produce no noticeable signs or mild symptoms, and the prevalence rate of CE has been found to be approximately 10%. Gynecologists and pathologists often do not focus much clinical attention on CE due to the time-consuming microscopic examinations necessary to diagnose CE, its mild clinical manifestations, and the benign nature of the disease...
December 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27689042/live-birth-in-a-woman-with-recurrent-implantation-failure-and-adenomyosis-following-transfer-of-refrozen-warmed-embryos
#11
Somayyeh Safari, Azita Faramarzi, Azam Agha-Rahimi, Mohammad Ali Khalili
The aim was to report a healthy live birth using re-vitrified-warmed cleavage-stage embryos derived from supernumerary warmed embryos after frozen embryo transfer (ET) in a patient with recurrent implantation failure (RIF). The case was a 39-year-old female with a history of polycystic ovarian syndrome and adenomyosis, along with RIF. After ovarian hyperstimulation, 33 cumulus-oocyte complexes were retrieved and fertilized with conventional in vitro fertilization and intracytoplasmic sperm injection. Because of the risk of ovarian hyperstimulation syndrome, 16 grade B and C embryos were vitrified...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27689041/psychological-distress-and-fertility-quality-of-life-fertiqol-in-infertile-korean-women-the-first-validation-study-of-korean-fertiqol
#12
Hee-Jun Chi, Il-Hae Park, Hong-Gil Sun, Jae-Won Kim, Kyeong-Ho Lee
OBJECTIVE: To investigate psychological distress and fertility quality of life (FertiQoL) in infertile Korean women, and to investigate whether a correlation exists between psychological distress and FertiQoL. METHODS: Participants in this study were made up of 141 infertile women and 65 fertile women. We conducted a survey on psychological distress (using the Depression Anxiety Stress Scales [DASS]-42 questionnaire) and administered a FertiQoL questionnaire. The levels of stress hormones (adrenocorticotropic hormone [ACTH] and cortisol) in serum were assessed...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27689040/pregnancy-rate-in-women-with-adenomyosis-undergoing-fresh-or-frozen-embryo-transfer-cycles-following-gonadotropin-releasing-hormone-agonist-treatment
#13
Chan Woo Park, Min Hye Choi, Kwang Moon Yang, In Ok Song
OBJECTIVE: To determine the preferred regimen for women with adenomyosis undergoing in vitro fertilization (IVF), we compared the IVF outcomes of fresh embryo transfer (ET) cycles with or without gonadotropin-releasing hormone (GnRH) agonist pretreatment and of frozen-thawed embryo transfer (FET) cycles following GnRH agonist treatment. METHODS: This retrospective study included 241 IVF cycles of women with adenomyosis from January 2006 to January 2012. Fresh ET cycles without (147 cycles, group A) or with (105 cycles, group B) GnRH agonist pretreatment, and FET cycles following GnRH agonist treatment (43 cycles, group C) were compared...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27689039/clinical-outcomes-of-single-versus-double-blastocyst-transfer-in-fresh-and-vitrified-warmed-cycles
#14
Jin Hee Eum, Jae Kyun Park, So Young Kim, Soo Kyung Paek, Hyun Ha Seok, Eun Mi Chang, Dong Ryul Lee, Woo Sik Lee
OBJECTIVE: Assisted reproductive technology has been associated with an increase in multiple pregnancies. The most effective strategy for reducing multiple pregnancies is single embryo transfer. Beginning in October 2015, the National Supporting Program for Infertility in South Korea has limited the number of embryos that can be transferred per in vitro fertilization (IVF) cycle depending on the patient's age. However, little is known regarding the effect of age and number of transferred embryos on the clinical outcomes of Korean patients...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27689038/percentage-of-motile-spermatozoa-at-22-hours-after-swim-up-procedure-an-indicator-for-intracytoplasmic-sperm-injection
#15
Taketo Inoue, Yukiko Yonezawa, Hironobu Sugimoto, Mikiko Uemura, Yuri Ono, Junji Kishi, Nobuyuki Emi, Yoshiyuki Ono
OBJECTIVE: The decision to use in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), or split insemination (IVF-ICSI) in the first cycle is based on the number of motile sperm. Hence, total fertilization failure (TFF) often occurs during IVF cycles, despite normozoospermia. To investigate whether the cumulative motile swim-up spermatozoa percentage at 22 hours post-insemination (MSPPI) is an indicator for ICSI, we analyzed TFF, fertilization, blastocyst development, chemical pregnancy, clinical pregnancy, and live birth rates...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27689037/the-effect-of-the-site-of-laser-zona-opening-on-the-complete-hatching-of-mouse-blastocysts-and-their-cell-numbers
#16
Usanee Sanmee, Waraporn Piromlertamorn, Teraporn Vutyavanich
OBJECTIVE: We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. METHODS: Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of the ICM, n=125), the trophectoderm (TE) group (zona opening performed opposite to the ICM, n=125) and the control group (no zona opening, n=125). RESULTS: The rate of complete hatching of the blastocysts was not significantly different in the ICM and the TE group (84...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27689036/comparison-of-static-culture-micro-vibration-culture-and-micro-vibration-culture-with-co-culture-in-poor-ovarian-responders
#17
Yong Soo Hur, Eun Kyung Ryu, San Hyun Yoon, Kyung Sil Lim, Won Don Lee, Jin Ho Lim
OBJECTIVE: This study was conducted to compare the effects of static culture, dynamic culture, and the combination of dynamic culture with specialized surfaces involving co-culture on human embryonic development. Embryos cultured using conventional static culture (SC) techniques served as a control group. We compared dynamic culture using micro-vibration culture (MVC) and micro-vibration with co-culture (MCoC), in which autologous cumulus cells were used as a specialized surface. METHODS: We conducted a chart review of patients who were treated between January 2011 and November 2014 in order to compare embryonic development rates and pregnancy rates among the groups...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27689035/the-meaning-of-anti-m%C3%A3-llerian-hormone-levels-in-patients-at-a-high-risk-of-poor-ovarian-response
#18
REVIEW
Hyun Jong Park, Geun Ho Lee, Du Sik Gong, Tae Ki Yoon, Woo Sik Lee
Measurements of ovarian reserve play an important role in predicting the clinical results of assisted reproductive technology (ART). The ideal markers of ovarian reserve for clinical applications should have high specificity in order to determine genuine poor responders. Basal follicle-stimulating hormone levels, antral follicle count, and serum anti-Müllerian hormone (AMH) levels have been suggested as ovarian reserve tests that may fulfill this requirement, with serum AMH levels being the most promising parameter...
September 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27358833/reproductive-outcomes-of-retransferring-retained-embryos-in-blastocyst-transfer-cycles
#19
Hyun Jeong Yi, Hwa Seon Koo, Sun Hwa Cha, Hye Ok Kim, Chan Woo Park, In Ok Song
OBJECTIVE: To determine the incidence of embryo retention (ER) in the transfer catheter following embryo transfer (ET) in blastocyst transfer and investigate whether retransferring retained embryos has an impact on reproductive outcomes in patients undergoing in vitro fertilization-ET. METHODS: We retrospectively analyzed the records of 1,131 blastocyst transfers, which comprised 223 single blastocyst transfer (SBT) and 908 double blastocyst transfer (DBT) cycles...
June 2016: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/27358832/application-of-serum-anti-m%C3%A3-llerian-hormone-levels-in-selecting-patients-with-polycystic-ovary-syndrome-for-in-vitro-maturation-treatment
#20
Hyun Ha Seok, Haengseok Song, Sang Woo Lyu, You Shin Kim, Dong Ryul Lee, Woo Sik Lee, Tae Ki Yoon
OBJECTIVE: The purpose of this study was to identify useful clinical factors for the identification of patients with polycystic ovary syndrome (PCOS) who would benefit from in vitro maturation (IVM) treatment without exhibiting compromised pregnancy outcomes. METHODS: A retrospective cohort study was performed of 186 consecutive patients with PCOS who underwent human chorionic gonadotropin-primed IVM treatment between March 2010 and March 2014. Only the first IVM cycle of each patient was included in this study...
June 2016: Clinical and Experimental Reproductive Medicine
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