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AMB Express

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https://www.readbyqxmd.com/read/29992518/characterization-of-two-pantoea-strains-isolated-from-extra-virgin-olive-oil
#1
Graziano Pizzolante, Miriana Durante, Daniela Rizzo, Marco Di Salvo, Salvatore Maurizio Tredici, Maria Tufariello, Angelo De Paolis, Adelfia Talà, Giovanni Mita, Pietro Alifano, Giuseppe Egidio De Benedetto
The olive oil is an unfavorable substrate for microbial survival and growth. Only few microorganisms use olive oil fatty acids as carbon and energy sources, and survive in the presence of olive oil anti-microbial components. In this study, we have evaluated the occurrence of microorganisms in 1-year-stored extra-virgin olive oil samples. We detected the presence of bacterial and yeast species with a recurrence of the bacterium Stenotrophomonas rhizophila and yeast Sporobolomyces roseus. We then assayed the ability of all isolates to grow in a mineral medium supplemented with a commercial extra-virgin olive oil as a sole carbon and energy source, and analyzed the utilization of olive oil fatty acids during their growth...
July 10, 2018: AMB Express
https://www.readbyqxmd.com/read/29992450/impacts-of-selenium-and-vitamin-e-supplementation-on-mrna-of-heat-shock-proteins-selenoproteins-and-antioxidants-in-broilers-exposed-to-high-temperature
#2
Shahnawaz Kumbhar, Alam Z Khan, Fahmida Parveen, Zaheer A Nizamani, Farman A Siyal, Mohamed E Abd El-Hack, Fang Gan, Yunhuan Liu, Muhammad Hamid, Sonia A Nido, Kehe Huang
The study was carried out to investigate the effect of dietary selenium (Se) and vitamin E (VE) supplementation on mRNA level of heat shock proteins, selenoproteins, and antioxidant enzyme activities in the breast meat of broilers under summer heat stress conditions. A total of 200 male broilers (Ross 308) of 1 day age were randomly separated into 4 groups in a complete randomized design and were given a basal diet (Control, 0.08 mg Se/kg diet) or basal diet supplemented with VE (250 mg/kg VE), sodium selenite (0...
July 10, 2018: AMB Express
https://www.readbyqxmd.com/read/29992363/a-comparative-study-of-the-enzymatic-hydrolysis-of-batch-organosolv-pretreated-birch-and-spruce-biomass
#3
Vijayendran Raghavendran, Christos Nitsos, Leonidas Matsakas, Ulrika Rova, Paul Christakopoulos, Lisbeth Olsson
A shift towards a sustainable and green society is vital to reduce the negative effects of climate change associated with increased CO2 emissions. Lignocellulosic biomass is both renewable and abundant, but is recalcitrant to deconstruction. Among the methods of pretreatment available, organosolv (OS) delignifies cellulose efficiently, significantly improving its digestibility by enzymes. We have assessed the hydrolysability of the cellulose-rich solid fractions from OS-pretreated spruce and birch at 2% w/v loading (dry matter)...
July 10, 2018: AMB Express
https://www.readbyqxmd.com/read/29978349/nonionic-surfactants-and-their-effects-on-asymmetric-reduction-of-2-octanone-with-saccharomyces-cerevisiae
#4
Yunquan Zheng, Liangbin Li, Xianai Shi, Zhijian Huang, Feng Li, Jianmin Yang, Yanghao Guo
In an aqueous buffer system, serious reverse and side reactions were found in the asymmetric reduction of 2-octanone with Saccharomyces cerevisiae. However, some nonionic surfactants added to the aqueous buffer system improved the bioreduction process by decreasing the reverse and side reaction rates in addition to effectively increasing the average positive reaction rate. Further, a shorter carbon chain length of hydrophilic or hydrophobic moieties in surfactants resulted in a higher yield of (S)-2-octanol...
July 6, 2018: AMB Express
https://www.readbyqxmd.com/read/29971620/poly-l-gamma-glutamic-acid-production-by-recombinant-bacillus-subtilis-without-pgsa-gene
#5
Kazuhisa Sawada, Hiroyuki Araki, Yasushi Takimura, Kenta Masuda, Yasushi Kageyama, Katsuya Ozaki, Hiroshi Hagihara
Poly-gamma-glutamic acid (PGA) is a promising bio-based polymer that shares many functions with poly (acrylic acid) and its derivatives. Thus, technologies for efficient production and molecular size control of PGA are required to expand the application of this useful biopolymer. In Bacillus strains, PGA is synthesized by the PgsBCA protein complex, which is encoded by the pgsBCA gene operon, otherwise is known as ywsC and ywtAB operons and/or capBCA operon. Hence, we investigated responsible components of the PgsBCA complex in B...
July 3, 2018: AMB Express
https://www.readbyqxmd.com/read/29971574/effects-of-physicochemical-parameters-on-volatile-sulphur-compound-formation-from-l-methionine-catabolism-by-non-growing-cells-of-kluyveromyces-lactis
#6
Yuyun Lu, Margarete Nawrath, Jingcan Sun, Shao-Quan Liu
The present study investigated for the first time the effects of various physicochemical parameters on the production of volatile sulphur compounds (VSCs) by non-growing cells of Kluyveromyces lactis supplemented with L-methionine. The results showed that the production of VSCs positively correlated with the cell biomass, but it seemed that no clear relationship with L-methionine concentration existed. Temperature and pH significantly affected the formation of VSCs with more production at 30 °C and pH 5, respectively...
July 3, 2018: AMB Express
https://www.readbyqxmd.com/read/29961206/engineering-e-coli-cell-surface-in-order-to-develop-a-one-step-purification-method-for-recombinant-proteins
#7
Hamidreza Fasehee, Amin Rostami, Fatemeh Ramezani, Gholamreza Ahmadian
Sortases are enzymes mostly found in Gram-positive bacteria which cleave proteins site-specifically. This feature makes them a promising tool in molecular biology and biotechnology. In this study, using bacterial surface display of recombinant proteins and ability of sortase A in site-specifically cleavage of the amino acid sequences, a novel method for one-step purification of recombinant proteins was developed. Using computational program tools, a chimeric protein containing a metallothionein (mt) and chitin binding domain (ChBD) was attached to the C-terminal domain of the truncated outer membrane protein A (Lpp'-ompA) using sortase recognition site (amino acid residues: LPQTG) as a separator...
June 30, 2018: AMB Express
https://www.readbyqxmd.com/read/29961200/long-term-nitrate-removal-through-methane-dependent-denitrification-microorganisms-in-sequencing-batch-reactors-fed-with-only-nitrate-and-methane
#8
Weiwei Li, Peili Lu, Fengguang Chai, Lilan Zhang, Xinkuan Han, Daijun Zhang
Denitrifying anaerobic methane oxidation (damo) bioprocesses can remove nitrate using methane as the electron donor, which gains great concern due to the current stringent discharge standard of nitrogen in wastewater treatment plants. To obtain an engineering acceptable nitrogen removal rate (NRR) and demonstrate the long-term stable ability of damo system under conditions of nitrate and methane, two sequencing batch reactors (SBRs) fed with only nitrate and methane were operated for more than 600 days at 30 °C...
June 30, 2018: AMB Express
https://www.readbyqxmd.com/read/29946801/genome-scale-metabolic-model-analysis-indicates-low-energy-production-efficiency-in-marine-ammonia-oxidizing-archaea
#9
Feiran Li, Wei Xie, Qianqian Yuan, Hao Luo, Peishun Li, Tao Chen, Xueming Zhao, Zhiwen Wang, Hongwu Ma
Marine ammonia-oxidizing archaea (AOA) play an important role in the global nitrogen cycle by obtaining energy for biomass production from CO2 via oxidation of ammonium. The isolation of Candidatus "Nitrosopumilus maritimus" strain SCM1, which represents the globally distributed AOA in the ocean, provided an opportunity for uncovering the contributions of those AOA to carbon and nitrogen cycles in ocean. Although several ammonia oxidation pathways have been proposed for SCM1, little is known about its ATP production efficiency...
June 27, 2018: AMB Express
https://www.readbyqxmd.com/read/29943167/linalool-citral-eugenol-and-thymol-control-of-planktonic-and-sessile-cells-of-shigella-flexneri
#10
Moisés Tomás Ngome, José Guilherme Lembi Ferreira Alves, Ana Cristina Freitas de Oliveira, Patrícia da Silva Machado, Olga Lucía Mondragón-Bernal, Roberta Hilsdorf Piccoli
The antimicrobial activity of linalool, citral, eugenol and thymol was determined in growth studies of both planktonic (PC) and biofilm cells (BC) Shigella flexneri. These components were evaluated either in isolation or in combinations using a sequential experimental strategy with Plackett & Burman and central composite rotational designs totaling 47 treatments. The minimum inhibitory concentration for PC was 0.125% (v v-1 ) for linalool and 0.5% (v v-1 ) for citral, eugenol and thymol. The biofilm minimum bactericidal concentration was 3 and 1% (v v-1 ) for linalool and citral, respectively, and 2% (v v-1 ) for eugenol and thymol...
June 25, 2018: AMB Express
https://www.readbyqxmd.com/read/29936673/molecular-cloning-expression-and-adhesion-analysis-of-silent-slpb-of-lactobacillus-acidophilus-ncfm
#11
Yuxing Guo, Xiangyue Li, Yao Yang, Zhen Wu, Xiaoqun Zeng, Fawze Nadari, Daodong Pan
The slpB gene of Lactobacillus acidophilus NCFM, which differs from the slpA gene and is silent under normal conditions, was successfully amplified and ligated to the corresponding available sites on a recombinant pET-28a vector. Then the pET-28a-slpB vector was transformed into Escherichia coli DH (DE3) and the fusion His-slpB protein was expressed by induction with 1 mM IPTG for 14 h at 37 °C. The resulting His-slpB protein (SB ) had a relative molecular weight of 48 kDa. It was purified using a Ni-NTA column and was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot contrastive analysis...
June 23, 2018: AMB Express
https://www.readbyqxmd.com/read/29936607/similarities-and-differences-in-gut-microbiome-composition-correlate-with-dietary-patterns-of-indian-and-chinese-adults
#12
Abhishek Jain, Xin Hui Li, Wei Ning Chen
The interaction between diet and gut microbiota, and ultimately their link to health, has turned into the concentration of huge research. However, this relationship still needs to be fully characterized, particularly in case of the Asian population. We compared the fecal bacterial diversity and composition of healthy Indian and Chinese adults, ages 22-35 years, using next-generation sequencing analysis on IlluminaHiSeq 2500 platform. Our analysis revealed unique community structure, dominant Firmicutes, Actinobacteria and underrepresented Bacteroides, of Indian and Chinese gut bacteria...
June 23, 2018: AMB Express
https://www.readbyqxmd.com/read/29923151/protein-profiles-of-bacteriophages-of-the-family-myoviridae-like-induced-on-m-haemolytica
#13
Renata Urban-Chmiel, Andrzej Wernicki, Jacek Wawrzykowski, Andrzej Puchalski, Anna Nowaczek, Marta Dec, Diana Stęgierska, Mohammed Mijbas Mohammed Alomari
The aim of study was to isolate, characterize and analyse the protein profiles of Myoviridae-like bacteriophages obtained from M. haemolytica using MALDI TOF mass spectrometry. The material consisted of the M. haemolytica reference strain ATCC® BAA410, reference serotypes A1, A2, A5, A6, A7, A9, and A11, and wild-type isolates of serotype A1. Bacteriophage morphology was examined with a transmission electron microscope. The proteins were separated in SDS-PAGE and two-dimensional electrophoresis and characterized by MALDI-TOF...
June 19, 2018: AMB Express
https://www.readbyqxmd.com/read/29923143/particle-attached-and-free-floating-pathogens-survival-kinetics-under-typical-stream-and-thermal-spring-temperature-conditions
#14
Yi Wang, Pramod Pandey, Yawen Zheng, Edward Robert Atwill, Gregory Pasternack
Improved understanding of pathogen survival in the stream environment is needed to enhance existing predictive models of stream pathogen populations. Further, the increasing use of thermal springs for bathing necessitates additional studies focused on not only typical streams but also thermal spring conditions, where water temperature is relatively higher than typical streams. This study was conducted to assess the survival of E. coli O157:H7 and Salmonella Typhimurium in stream water under free floating and particle-attached conditions at a range of temperature...
June 19, 2018: AMB Express
https://www.readbyqxmd.com/read/29922919/rapid-and-simple-detection-of-endospore-counts-in-probiotic-bacillus-cultures-using-dipicolinic-acid-dpa-as-a-marker
#15
Xiao-Sheng Liang, Chun Liu, Zhu Long, Xiao-Hua Guo
Spore counting in probiotic Bacillus cultures using dipicolinic acid (DPA) as a marker was studied for developing a rapid and simple detection method. The newly developed method is based on the fluorescence enhancement by a new chelating agent, which forms a complex with EuCl3 and DPA. The results showed that 1,2-cyclohexanediamine-N,N,N'N'-tetraacetic acid (CyDTA) greatly enhanced the fluorescence intensity in all selected chelating agents. The optimal composition of the fluorescence complex DPA-Eu-CyDTA had a detection limit of 0...
June 19, 2018: AMB Express
https://www.readbyqxmd.com/read/29909539/development-of-a-species-specific-taqman-mgb-real-time-pcr-assay-to-quantify-olsenella-scatoligenes-in-pigs-offered-a-chicory-root-based-diet
#16
Xiaoqiong Li, Bent Borg Jensen, Ole Højberg, Samantha Joan Noel, Nuria Canibe
Olsenella scatoligenes is the only skatole-producing bacterium isolated from the pig gut. Skatole, produced from microbial degradation of l-tryptophan, is the main contributor to boar taint, an off-odor and off-flavor taint, released upon heating meat from some entire male pigs. An appropriate method for quantifying O. scatoligenes would help investigating the relationship between O. scatoligenes abundance and skatole concentration in the pig gut. Thus, the present study aimed at developing a TaqMan-MGB probe-based, species-specific qPCR assay for rapid quantification of O...
June 16, 2018: AMB Express
https://www.readbyqxmd.com/read/29909506/the-influence-of-in-vitro-pectin-fermentation-on-the-human-fecal-microbiome
#17
So-Jung Bang, Gayoung Kim, Mi Young Lim, Eun-Ji Song, Dong-Hyun Jung, Jun-Seok Kum, Young-Do Nam, Cheon-Seok Park, Dong-Ho Seo
Pectin is a complex dietary fiber and a prebiotic. To investigate pectin-induced changes in the gut microbiome and their effects on the short chain fatty acids (SCFAs) production, we performed in vitro pectin fermentation using the feces of three Korean donors. The pectin degradations in all three donors were observed. While the donors displayed differences in baseline gut microbiota composition, commonly increased bacteria after pectin fermentation included Lachnospira, Dorea, Clostridium, and Sutterella. Regarding SCFAs, acetate levels rapidly increased with incubation with pectin, and butyrate levels also increased after 6 h of incubation...
June 16, 2018: AMB Express
https://www.readbyqxmd.com/read/29896682/improving-pha-production-in-a-sbr-of-coupling-pha-storing-microorganism-enrichment-and-pha-accumulation-by-feed-on-demand-control
#18
Shanwen Zeng, Fuzhong Song, Peili Lu, Qiang He, Daijun Zhang
With volatile fatty acids as substrates, the typical polyhydroxyalkanoates (PHAs) production by mixed culture always includes two steps: PHA-storing culture enrichment via aerobic dynamic feeding strategy and PHA accumulation under nutrient-limited condition. To simplify the PHA-production steps, the enrichment and accumulation step were coupled in a SBR. At start-up period, to investigate the effect of settling selection, one acetate-fed SBR was operated by settling selection-double growth limitation (SS-DGL) strategy, while the other was operated by DGL strategy...
June 12, 2018: AMB Express
https://www.readbyqxmd.com/read/29896640/purification-and-characterization-of-neutral-protease-from-aspergillus-oryzae-y1-isolated-from-naturally-fermented-broad-beans
#19
Xiao-Lin Ao, Xi Yu, Ding-Tao Wu, Chao Li, Tong Zhang, Shu-Liang Liu, Shu-Juan Chen, Li He, Kang Zhou, Li-Kou Zou
The strain Y1, with a notably high production of neutral protease, was isolated from naturally fermented broad beans and subsequently identified as Aspergillus oryzae, through the analysis of its morphology characteristics and 18S rDNA sequence. Naturally fermented broad beans are the main raw material in Sichuan broad-bean sauce. The neutral protease from Aspergillus oryzae Y1 was purified using ammonium sulphate precipitation and DEAE-Sepharose Fast Flow chromatography, which resulted in a 10.0-fold increase in the specific activity (2264...
June 12, 2018: AMB Express
https://www.readbyqxmd.com/read/29873028/an-alkaline-and-surfactant-tolerant-lipase-from-trichoderma-lentiforme-accc30425-with-high-application-potential-in-the-detergent-industry
#20
Yuzhou Wang, Rui Ma, Shigui Li, Mingbo Gong, Bin Yao, Yingguo Bai, Jingang Gu
Alkaline lipases with adaptability to low temperatures and strong surfactant tolerance are favorable for application in the detergent industry. In the present study, a lipase-encoding gene, TllipA, was cloned from Trichoderma lentiforme ACCC30425 and expressed in Pichia pastoris GS115. The purified recombinant TlLipA was found to have optimal activities at 50 °C and pH 9.5 and retain stable over the pH range of 6.0-10.0 and 40 °C and below. When using esters of different lengths as substrates, TlLipA showed preference for the medium length p-nitrophenyl octanoate...
June 5, 2018: AMB Express
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