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AMB Express

Mohammad Hossien Salmani, Mohammad Hassan Ehrampoush, Mehdi Mokhtari, Bahar Eftekhar
Most of the medicinal compounds are entered no change into municipal wastewater and more than 90% of it remains in the wastewater. Mesoporous silica is known as thermally stable materials with controllable porosity and morphology. It is specified that these materials possess external and internal surfaces that can be selectively adsorbed the various compounds. In the present study, the synthesized mesoporous silica was studied to remove bisacodyl from polluted solutions. Mesoporous silica was synthesized by simple chemical method from tetraethylorthosilicate, ethanol (C2 H5 OH) and deionized water at 70 °C temperature...
September 10, 2018: AMB Express
Jaewon Hur, Thi Thanh Hanh Nguyen, Namhyeon Park, Jeesoo Kim, Doman Kim
Quinoa is a pseudocereal that contains high quality protein, minerals, vitamins, polyphenols, and phytosterols. In this study, quinoa was fermented by Rhizopus oligosporus (R. oligosporus) up to 5 days and the functional compounds (L-carnitine, GABA, vanillic acid and gallic acid) were analyzed by LC/MS. The amounts of L-carnitine and GABA were 0.13 mg/kg and 540 mg/kg for nonfermented quinoa (NF), 3.15 mg/kg and 1040 mg/kg for fermented quinoa at 3 days (3F), and 1.54 mg/kg and 810 mg/kg for fermented quinoa at 5 days (5F)...
September 10, 2018: AMB Express
Yang Hu, Fengxian Sun, Weiying Liu
With the developments in taxonomy, the classically used highly conserved 16S rRNA molecular marker has shown some disadvantages among closely related species. For further taxonomic studies of the prokaryotes, specific PCR primers were designed from two conserved regions in the amino acid sequences of the 70-kDa heat shock protein sourced from 20 different genera in actinomycetes. These were used for the amplification of the hsp70 genes in 16 Streptomyces strains. Then, we investigated the phylogenetic relationships among these Streptomyces strains and compared the tree topology based on the hsp70 gene with those based on the previously used markers (16S rRNA and gyrB)...
September 10, 2018: AMB Express
Aneesh Balakrishna Pillai, Arjun Jaya Kumar, Harikrishnan Kumarapillai
Poly(3-hydroxybutyrate) (PHB) is a bacterial polymer of great commercial importance due to its properties similar to polypropylene. With an aim to develop a recombinant system for economical polymer production, PHB biosynthesis genes from Bacillus aryabhattai PHB10 were cloned in E. coli. The recombinant cells accumulated a maximum level of 6.22 g/L biopolymer utilizing glycerol in shake flasks. The extracted polymer was confirmed as PHB by GC-MS and NMR analyses. The polymer showed melting point at 171 °C, thermal stability in a temperature range of 0-140 °C and no weight loss up to 200 °C...
September 4, 2018: AMB Express
Jiafu Huang, Yixin Ou, Danfeng Zhang, Guoguang Zhang, Yutian Pan
In the present study, conditions for Bacillus mucilaginous fermentation using Agaricus bisporus wastewater as culture medium were optimized. We analyzed the total number of living B. mucilaginous in the fermentation broth using multispectral imaging flow cytometry. Single-factor experiments were carried out, where a Plackett-Burman design was used to screen out three factors from the original six factors of processing wastewater solubility, initial pH, inoculum size, liquid volume, culture temperature, and rotation speed that affected the total number of viable B...
August 31, 2018: AMB Express
Barry Kamira, Lei Lei Shi, Li Min Fan, Cong Zhang, Yao Zheng, Chao Song, Shun Long Meng, Geng Dong Hu, Xu Wen Bing, Zhang Jia Chen, Pao Xu
The discovery of aerobic and anammox bacteria capable of generating methane in bio-filters in freshwater aquaculture systems is generating interest in studies to understand the activity, diversity, distribution and roles of these environmental bacteria. In this study, we used microbial enrichment of bio-filters to assess their effect on water quality. Profiles of ammonia-oxidizing bacterial communities generated using nested PCR methods and DGGE were used to assess the expression of 16S rRNA genes using DNA sequencing...
August 28, 2018: AMB Express
Yosuke Kobayashi, Takehiko Sahara, Satoru Ohgiya, Yoichi Kamagata, Kazuhiro E Fujimori
The pentose phosphate pathway (PPP) plays an important role in the synthesis of ribonucleotides and aromatic amino acids. During bioethanol production from cellulosic biomass composed mainly of D-glucose and D-xylose, the PPP is also involved in xylose metabolism by engineered Saccharomyces cerevisiae. Although the activities and thermostabilities of the four PPP enzymes (transaldolase: TAL1, transketolase: TKL1, ribose-5-phosphate ketol-isomerase: RKI1 and D-ribulose-5-phosphate 3-epimerase: RPE1) can affect the efficiency of cellulosic ethanol production at high temperatures, little is known about the suitable expression levels of these PPP genes...
August 27, 2018: AMB Express
Tae-Kyung Lee, Thi Thanh Hanh Nguyen, Namhyeon Park, So-Hyung Kwak, Jeesoo Kim, Shina Jin, Gyu-Min Son, Jaewon Hur, Jong-In Choi, Doman Kim
L-Carnitine is an essential compound that shuttles long chain fatty acids into mitochondria. The objective of this study was to produce L-carnitine enriched oyster mushroom (Pleurotus ostreatus) using common buckwheat fermented by Rhizopus oligosporus. Mushroom grown on common buckwheat medium contained 9.9-23.9% higher L-carnitine (186.3 mg/kg) than those grown on basal medium without any buckwheat addition. Those grown on fermented common buckwheat medium contained the highest L-carnitine content (201.2 mg/kg)...
August 27, 2018: AMB Express
Jonas Rönnander, Joel Ljunggren, Erik Hedenström, Sandra Ann Ingela Wright
Vanillin is an aromatic aldehyde found as a component of lignocellulosic material, and in the cured pods of orchidaceae plants. Like other phenolic substances, vanillin has antimicrobial activity and can be extracted from lignin either by a thermo-chemical process or through microbial degradation. Vanillin, can serve as a model monomer in biodegradation studies of lignin. In the present study, a yeast isolated from decaying wood on the Faroe Islands, was identified as Cystobasidium laryngis strain FMYD002, based on internal transcribed spacer sequence analysis...
August 24, 2018: AMB Express
Chotima Potisap, Md Abdul Wadud Khan, Atcha Boonmee, Jorge L M Rodrigues, Surasakdi Wongratanacheewin, Rasana W Sermswan
Burkholderia pseudomallei is a Gram-negative bacterium found in soil and the causative agent of a severe disease in humans and animals known as melioidosis. It is intrinsically resistant to many antibiotics and has been reported resistant to the drugs of choice; ceftazidime. Microbial communities in soil in the presence and absence of B. pseudomallei were investigated using metagenomics approach. The variation in bacterial species diversity was significantly higher in soil samples without B. pseudomallei. Abundances of phyla Actinobacteria and Firmicutes were found significantly higher in B...
August 24, 2018: AMB Express
Jing Su, Jennifer Noro, Jiajia Fu, Qiang Wang, Carla Silva, Artur Cavaco-Paulo
Laccases have been reported for their ability to eliminate hazardous phenolic compounds by oxidative polymerization. The exploitation of the oxidative behavior of different laccase forms, namely free/native, free/PEGylated, immobilized/native and immobilized/PEGylated, was assessed in this study. We found that PEGylated and immobilized laccase forms have differentiated catalytic behavior revealing distinct conversion rates and differentiated poly(catechol) chains, as confirmed by UV-Visible spectroscopy, by the total content of OH groups and by MALDI-TOF spectroscopy...
August 22, 2018: AMB Express
Nasser Nassiri Koopaei, Parissa Khadiv-Parsi, Mohammad Reza Khoshayand, Mohammad Ali Mazlomi, Abbas Kebriaeezadeh, Hamid Moloudian, Roya Solhi, Mahdi Aminian
High yield recombinant protein production is highly desirable for biotechnological purposes. In the design of recombinant expression conditions, a number of essential central elements such as expression strain, type of medium, bioprocess optimization, and mathematical modeling should be considered. Well-designed industrial scale production of one recombinant protein with optimized influential parameters and yield can address the cost and production reproducibility issues. In the present study, statistical experimental design methodology was used to investigate the effect of fermentation conditions (dissolved oxygen, IPTG, and temperature) on rPDT production by Escherichia coli...
August 22, 2018: AMB Express
Natalia Kwiatos, Marzena Jędrzejczak-Krzepkowska, Bartosz Strzelecki, Stanisław Bielecki
Clean coal technologies (e.g. coal biosolubilization) are of essential value, especially in Europe, where coal is the national wealth and other energy sources like crude oil are not available. Fusarium oxysporum LOCK 1134, the strain isolated from brown coal, efficiently biosolubilizes lignite. The obtained liquefied products contain 50% less sulfur and over 99% less mercury than the crude coal. Moreover, the liquefied coal can be modified further by laccase. In this study F. oxysporum laccase was expressed in Pichia pastoris for the first time and was assessed as an additional agent for coal degradation...
August 22, 2018: AMB Express
Lisa Marie Halka, Christian Nowacki, Alica Kleinschmidt, Kevin Koenen, Rolf Wichmann
Fusicocca-2,10(14)-diene (FCdiene) is a diterpene which is interesting as a precursor of the anti-cancer drug fusicoccin A and therefore for pharmaceutical applications. Production of FCdiene using a genetically modified Saccharomyces cerevisiae has been previously demonstrated with batch cultivations with a product concentration up to 10 mg/L. However, it is widely known that fed-batch processes can significantly improve product titer in yeast fermentations. This study focuses on the establishment of fed-batch fermentation for FCdiene production because fed-batch cultivations using FeedBeads® indicated that limiting glucose supply could increase yields of biomass (1...
August 22, 2018: AMB Express
Jaejung Kim, Xiao Mei Lyu, Jaslyn Jie Lin Lee, Guili Zhao, Seow Fong Chin, Liang Yang, Wei Ning Chen
Utilization of algicidal bacteria as a biological agent have been receiving significant interest for controlling harmful algal blooms. While various algicidal bacterial strains have been identified, limited studies have explored the influence of bacterial culture conditions on its algicidal activity. Here, the effect of oxygen on the algicidal activity of a novel bacterium JK12, against a model diatom, Phaeodactylum tricornutum (P. tricornutum) was studied. Strain JK12 showed high algicidal activity against P...
August 20, 2018: AMB Express
Amandine Deroite, Jean-Luc Legras, Peggy Rigou, Anne Ortiz-Julien, Sylvie Dequin
Saccharomyces cerevisiae × Saccharomyces kudriavzevii hybrids are typically used for white wine fermentation because of their cryotolerance. One group of these hybrids presents a unique ability to release thiol varietal aroma products as well as excessive amounts of acetic acid under specific conditions, which is detrimental for wine organoleptic quality. The aim of this work is to better assess the effects of lipids, sugar concentrations and temperature on the production of acetic acid and thiols during wine fermentation...
August 10, 2018: AMB Express
Lakshminarayana Turuvekere Sadguruprasad, Madhusudhan Basavaraj
The awareness regarding use of probiotics for health benefits has created a huge market worldwide. The use of probiotic cultures and its success in commercial scale is relying on the techniques employed to ensure that the potential cultures with desired functional activity are stable and viable for longer period. The present study was undertaken to statistically evaluate the effect of lyoprotectant in the suspension media containing sucrose and reconstituted skim milk on the viability of Lactobacillus acidophilus strains...
August 10, 2018: AMB Express
Vesna Milićević, Sonja Radojičić, Jasna Kureljušić, Milanko Šekler, Ksenija Nešić, Ljubiša Veljović, Jelena Maksimović Zorić, Vladimir Radosavljević
Considering the intensive trading nowadays, the honey from the local market was tested for the presence of the six most common bee viruses. To prove the suitability of honey as a sample for the bee viruses detection, the set of different sample types taken directly from the hives we comparatively tested. The study included 30 samples of domestic and 5 samples of imported honey. Additionally, we tested 40 sets of samples including live bees, dead bees, and the honey taken from four apiaries for the evaluation of honey suitability for the virus detection, Two out of the six most common bee viruses were detected in the samples of honey from the market...
August 7, 2018: AMB Express
Bruno Vasconcelos, José Carlos Teixeira, Giuliano Dragone, José António Teixeira
The constant growing demand for vegetable oil for biodiesel and food is raising many environmental concerns about the sustainability of its production based on crops. Oleaginous yeasts show great potential to end with those concerns due to their high lipid productivity in small areas. To evaluate their productivity in lipids, an efficient and reproducible extraction process should be used. As no standard extraction process is available for the extraction of yeast lipids, an optimized extraction process is presented...
August 6, 2018: AMB Express
Tomasz Kowalczyk, Aneta Gerszberg, Paulina Durańska, Róża Biłas, Katarzyna Hnatuszko-Konka
Brassica oleracea var. botrytis, a very popular crop grown for its edible inflorescence, is bred only as a mutated annual cultivar and does not naturally occur in environment. Since cauliflower is still described as the most troublesome of all the B. oleracea vegetables regarding transformation processes, it is fully justified to focus on the improvement of tools for its genetic modifications. Here, we present a successful protocol for genetic transformation of cauliflower employing the process of agroinfection...
August 6, 2018: AMB Express
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