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AMB Express

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https://www.readbyqxmd.com/read/29789978/diauxic-growth-of-clostridium-acetobutylicum-atcc-824-when-grown-on-mixtures-of-glucose-and-cellobiose
#1
Felipe Buendia-Kandia, Emmanuel Rondags, Xavier Framboisier, Guillain Mauviel, Anthony Dufour, Emmanuel Guedon
Clostridium acetobutylicum, a promising organism for biomass transformation, has the capacity to utilize a wide variety of carbon sources. During pre-treatments of (ligno) cellulose through thermic and/or enzymatic processes, complex mixtures of oligo saccharides with beta 1,4-glycosidic bonds can be produced. In this paper, the capability of C. acetobutylicum to ferment glucose and cellobiose, alone and in mixtures was studied. Kinetic studies indicated that a diauxic growth occurs when both glucose and cellobiose are present in the medium...
May 22, 2018: AMB Express
https://www.readbyqxmd.com/read/29785529/isolation-and-characterization-of-bacillus-sp-gfp-2-a-novel-bacillus-strain-with-antimicrobial-activities-from-whitespotted-bamboo-shark-intestine
#2
Jia Wu, Guoqiang Xu, Yangyang Jin, Cong Sun, Li Zhou, Guodong Lin, Rong Xu, Ling Wei, Hui Fei, Dan Wang, Jianqing Chen, Zhengbing Lv, Kuancheng Liu
The abuse of antibiotics and following rapidly increasing of antibiotic-resistant pathogens is the serious threat to our society. Natural products from microorganism are regarded as the important substitution antimicrobial agents of antibiotics. We isolated a new strain, Bacillus sp. GFP-2, from the Chiloscyllium plagiosum (Whitespotted bamboo shark) intestine, which showed great inhibitory effects on the growth of both Gram-positive and Gram-negative bacteria. Additionally, the growth of salmon was effectively promoted when fed with inactivated strain GFP-2 as the inhibition agent of pathogenic bacteria...
May 22, 2018: AMB Express
https://www.readbyqxmd.com/read/29785587/a-study-on-the-use-of-strain-specific-and-homologous-promoters-for-heterologous-expression-in-industrial-saccharomyces-cerevisiae-strains
#3
Daniel Pereira de Paiva, Tiago Benoliel Rocha, Marciano Regis Rubini, André Moraes Nicola, Viviane Castelo Branco Reis, Fernando Araripe Gonçalves Torres, Lidia Maria Pepe de Moraes
Polymorphism is well known in Saccharomyces cerevisiae strains used for different industrial applications, however little is known about its effects on promoter efficiency. In order to test this, five different promoters derived from an industrial and a laboratory (S288c) strain were used to drive the expression of eGFP reporter gene in both cells. The ADH1 promoter (P ADH1 ) in particular, which showed more polymorphism among the promoters analyzed, also exhibited the highest differences in intracellular fluorescence production...
May 21, 2018: AMB Express
https://www.readbyqxmd.com/read/29785517/optimization-and-enhancement-of-textile-reactive-remazol-black-b-decolorization-and-detoxification-by-environmentally-isolated-ph-tolerant-pseudomonas-aeruginosa-ky284155
#4
Rasha A Hashem, Reham Samir, Tamer M Essam, Amal E Ali, Magdy A Amin
Azo dyes are complex derivatives of diazene used in food and textile manufacture. They are highly recalcitrant compounds, and account for severe environmental and health problems. Different strains of Pseudomonas species were isolated from textile wastewater effluents. The bioconversion of Remazol black B (a commonly used water soluble dye) by Pseudomonas aeruginosa was observed in static conditions. The bio-decolorization process was optimized by a multi factorial Plackett-Burman experimental design. Decolorization of 200 mg L-1 reached 100% in 32 h...
May 21, 2018: AMB Express
https://www.readbyqxmd.com/read/29785487/rapid-monitoring-of-the-target-protein-expression-with-a-fluorescent-signal-based-on-a-dicistronic-construct-in-escherichia-coli
#5
Chang-Ye Hui, Yan Guo, Wen Zhang, Xian-Qing Huang
Real-time quantification of recombinant proteins is important in studies on fermentation engineering, cell engineering, etc. Measurement of the expression level of heterologous proteins in bacterial fermentation broth has traditionally relied on time-consuming and labor-intensive procedures, such as polyacrylamide gel electrophoresis, immunoblot analysis, and biological activity assays. We describe a simple, fast, and high sensitive assay for detecting heterologous proteins production in bacteria either at the overall level (fluorescence spectrophotometry) or at the individual level (fluorescence microscopic image) in this study...
May 21, 2018: AMB Express
https://www.readbyqxmd.com/read/29774473/genomic-and-probiotic-characterization-of-sjp-snu-strain-of-pichia-kudriavzevii
#6
Seung-Min Hong, Hyuk-Joon Kwon, Se-Joon Park, Won-Jin Seong, Ilhwan Kim, Jae-Hong Kim
The yeast strain SJP-SNU was investigated as a probiotic and was characterized with respect to growth temperature, bile salt resistance, hydrogen sulfide reducing activity, intestinal survival ability and chicken embryo pathogenicity. In addition, we determined the complete genomic and mitochondrial sequences of SJP-SNU and conducted comparative genomics analyses. SJP-SNU grew rapidly at 37 °C and formed colonies on MacConkey agar containing bile salt. SJP-SNU reduced hydrogen sulfide produced by Salmonella serotype Enteritidis and, after being fed to 4-week-old chickens, could be isolated from cecal feces...
May 17, 2018: AMB Express
https://www.readbyqxmd.com/read/29777449/potential-of-bacillus-subtilis-lipopeptides-in-anti-cancer-i-induction-of-apoptosis-and-paraptosis-and-inhibition-of-autophagy-in-k562-cells
#7
Haobin Zhao, Lu Yan, Xiaoguang Xu, Chunmei Jiang, Junling Shi, Yawen Zhang, Li Liu, Shuzhen Lei, Dongyan Shao, Qingsheng Huang
The lipopeptide iturin from Bacillus subtilis has been found to have a potential inhibitory effect on breast cancer, alveolar adenocarcinoma, renal carcinoma, and colon adenocarcinoma. In this study, the potential of B. subtilis lipopeptides (a mixture of iturin homologues, concentration of 42.75%) to inhibit chronic myelogenous leukemia was evaluated using K562 myelogenous leukemia cells. The results showed that the lipopeptides could completely inhibit the growth of K562 at 100 μM, with an IC50 value of 65...
May 9, 2018: AMB Express
https://www.readbyqxmd.com/read/29744607/stepwise-partially-overlapping-primer-based-pcr-for-genome-walking
#8
Kunpeng Chang, Qiong Wang, Xiaofei Shi, Shuixing Wang, Hongjing Wu, Lijuan Nie, Haixing Li
A stepwise partially overlapping primer-based PCR (SWPOP-PCR) method for isolating flanking unknown DNA regions was developed, which comprises three rounds of nested PCRs sequentially driven by SWPOP primer-nested specific primer pairs. SWPOP primer set is characterized by a partial overlap of 10 bp with 3'-part of the latter primer is identical to 5'-part of the former one, which makes the SWPOP primer in use anneal to SWPOP site of the prior PCR product only at relatively low temperature. For each PCR, target single-stranded DNA primed by the SWPOP primer in the exclusive one low-stringency cycle is converted into double-stranded form in the following high-stringency cycle due to the presence of a perfect annealing site for the specific primer...
May 9, 2018: AMB Express
https://www.readbyqxmd.com/read/29744605/microbial-diversity-and-ecotoxicity-of-sediments-3-years-after-the-jiaozhou-bay-oil-spill
#9
Wei Gao, Xiaofei Yin, Tiezhu Mi, Yiran Zhang, Faxiang Lin, Bin Han, Xilong Zhao, Xiao Luan, Zhisong Cui, Li Zheng
In 2013, the "Qingdao oil pipeline explosion" released an estimated 2000 tons of oil into the environment. Sediment samples were collected from ten sites in Jiaozhou Bay and Shilaoren Beach to evaluate the influence of the spilled oil on the benthic environment 3 years after the oil spill accident. The compositions of oil, bacterial diversity and biotoxicity were examined in this study. The results showed that the concentration of total petroleum hydrocarbons (TPHs) peaked near the oil leak point and gradually decreased along the coastline, ranging from 21...
May 9, 2018: AMB Express
https://www.readbyqxmd.com/read/29730843/effect-of-decanoic-acid-and-10-hydroxydecanoic-acid-on-the-biotransformation-of-methyl-decanoate-to-sebacic-acid
#10
Yohanes Eko Chandra Sugiharto, Heeseok Lee, Annur Dyah Fitriana, Hyeokwon Lee, Wooyoung Jeon, Kyungmoon Park, Jungoh Ahn, Hongweon Lee
Biotransformation of fatty acid methyl esters to dicarboxylic acids has attracted much attention in recent years; however, reports of sebacic acid production using such biotransformation remain few. The toxicity of decanoic acid is the main challenge for this process. Decane induction has been reported to be essential to activate the enzymes involved in the α,ω-oxidation pathway before initiating the biotransformation of methyl decanoate to sebacic acid. However, we observed the accumulation of intermediates (decanoic acid and 10-hydroxydecanoic acid) during the induction period...
May 5, 2018: AMB Express
https://www.readbyqxmd.com/read/29730829/the-influence-of-oxygen-and-methane-on-nitrogen-fixation-in-subarctic-sphagnum-mosses
#11
Martine A R Kox, Sanni L Aalto, Timo Penttilä, Katharina F Ettwig, Mike S M Jetten, Maartje A H J van Kessel
Biological nitrogen fixation is an important source of bioavailable nitrogen in Sphagnum dominated peatlands. Sphagnum mosses harbor a diverse microbiome including nitrogen-fixing and methane (CH4 ) oxidizing bacteria. The inhibitory effect of oxygen on microbial nitrogen fixation is documented for many bacteria. However, the role of nitrogen-fixing methanotrophs in nitrogen supply to Sphagnum peat mosses is not well explored. Here, we investigated the role of both oxygen and methane on nitrogen fixation in subarctic Sphagnum peat mosses...
May 5, 2018: AMB Express
https://www.readbyqxmd.com/read/29728880/directed-evolution-of-the-bacterial-endo-%C3%AE-1-4-glucanase-from-streptomyces-sp-g12-towards-improved-catalysts-for-lignocellulose-conversion
#12
Davide Agostino Cecchini, Olimpia Pepe, Anna Pennacchio, Massimo Fagnano, Vincenza Faraco
With the aim to develop biocatalysts for enhanced hydrolysis of (hemi)cellulose into monosaccharides, random diversity by directed evolution was introduced in the gene coding for the endo-β-1,4-glucanase from Streptomyces sp. G12 which had been recombinantly expressed in Escherichia coli and named rCelStrep. The main objectives were therefore to set up a complete strategy for creation and automated screening of rCelStrep evolved direct mutants and to apply it to generate and screen a library of 10,000 random mutants to select the most active variants...
May 5, 2018: AMB Express
https://www.readbyqxmd.com/read/29728787/azospirillum-benefits-that-go-far-beyond-biological-nitrogen-fixation
#13
REVIEW
Josiane Fukami, Paula Cerezini, Mariangela Hungria
The genus Azospirillum comprises plant-growth-promoting bacteria (PGPB), which have been broadly studied. The benefits to plants by inoculation with Azospirillum have been primarily attributed to its capacity to fix atmospheric nitrogen, but also to its capacity to synthesize phytohormones, in particular indole-3-acetic acid. Recently, an increasing number of studies has attributed an important role of Azospirillum in conferring to plants tolerance of abiotic and biotic stresses, which may be mediated by phytohormones acting as signaling molecules...
May 4, 2018: AMB Express
https://www.readbyqxmd.com/read/29721735/evaluation-of-anthocyanins-in-aronia-melanocarpa-bsa-binding-by-spectroscopic-studies
#14
Jie Wei, Dexin Xu, Xiao Zhang, Jing Yang, Qiuyu Wang
The interaction between Anthocyanins in Aronia melanocarpa (AMA) and bovine serum albumin (BSA) were studied in this paper by multispectral technology, such as fluorescence quenching titration, circular dichroism (CD) spectroscopy and Fourier transform infrared spectroscopy (FTIR). The results of the fluorescence titration revealed that AMA could strongly quench the intrinsic fluorescence of BSA by static quenching. The apparent binding constants KSV and number of binding sites n of AMA with BSA were obtained by fluorescence quenching method...
May 2, 2018: AMB Express
https://www.readbyqxmd.com/read/29721676/enhanced-hypocrellin-production-via-coexpression-of-alpha-amylase-and-hemoglobin-genes-in-shiraia-bambusicola
#15
Ruijie Gao, Huaxiang Deng, Zhengbing Guan, Xiangru Liao, Yujie Cai
Shiraia bambusicola is an important and valuable macrofungus and hypocrellins are its main secondary metabolites which have been widely applied in many medical fields. However, during SSF process of this filamentous fungus, use ratio of corn substrate and dissolved oxygen supply are two main limiting factors, which influence production cost, yield and product quality. To solve these problems, overexpressions of amy365-1 and vgb in S. bambusicola were investigated and three overexpression transformants were constructed...
May 2, 2018: AMB Express
https://www.readbyqxmd.com/read/29713843/a-co-utilization-strategy-to-consume-glycerol-and-monosaccharides-by-rhizopus-strains-for-fumaric-acid-production
#16
Sylwia Kowalczyk, Elwira Komoń-Janczara, Agnieszka Glibowska, Adam Kuzdraliński, Tomasz Czernecki, Zdzisław Targoński
The ability of Rhizopus oryzae to produce fumaric acid in the presence of glycerol and/or various monosaccharides as carbon sources was examined for seventeen different strains of this fungi. These strains were tested in shake-flask cultures on media containing glycerol and seven different carbohydrates, including glucose, fructose, galactose, mannose, xylose, arabinose, and rhamnose. An interesting and applicationally useful phenomenon was observed. This work presents a new approach to the conventional microbiological method of producing fumaric acid...
April 30, 2018: AMB Express
https://www.readbyqxmd.com/read/29713833/astragalus-affects-fecal-microbial-composition-of-young-hens-as-determined-by-16s-rrna-sequencing
#17
Hongxing Qiao, Liheng Zhang, Hongtao Shi, Yuzhen Song, Chuanzhou Bian
The gut microbiota play important roles in the degradation of chemical compounds of herbal medicines (HMs). However, little information regarding the interplay between HMs and the gut microbiota is available. Thus, the aim of this study was to investigate the composition of the fecal microbiota of young (age, 11 weeks) hens fed a conventional diet containing a crude Astragalus (0.5%) additive for 21 days (group A) vs. controls (group B) that were fed only conventional feed. The fecal contents of 14-week-old hens were collected for DNA extraction, and then the V3 and V4 hyper-variable regions of the 16S rRNA gene were amplified and analyzed using high-throughput sequencing technology...
April 30, 2018: AMB Express
https://www.readbyqxmd.com/read/29704067/microbial-community-and-diversity-in-the-feces-of-sichuan-takin-budorcas-taxicolor-tibetana-as-revealed-by-illumina-miseq-sequencing-and-quantitative-real-time-pcr
#18
Hui Zhu, Dong Zeng, Ning Wang, Li-Li Niu, Yi Zhou, Yan Zeng, Xue-Qin Ni
The Sichuan takin (Budorcas taxicolor tibetana) is a rare and endangered ruminant distributed in the eastern Himalayas. However, little information is available regarding the intestinal microbiota of the takin. In this study, Illumina Miseq platform targeting the V4 region of the 16S rRNA was employed to characterize microbial community and diversity in the feces of wild (n = 6) and captive takins (n = 6). The takin exhibited an intestinal microbiota dominated by three phyla: Firmicutes (57.4%), Bacteroidetes (24...
April 27, 2018: AMB Express
https://www.readbyqxmd.com/read/29696483/quantification-of-amino-acids-and-peptides-in-an-ionic-liquid-based-aqueous-two-phase-system-by-lc-ms-analysis
#19
Sebastian Oppermann, Christina Oppermann, Miriam Böhm, Toni Kühl, Diana Imhof, Udo Kragl
Aqueous two-phase systems (ATPS) occur by the mixture of two polymers or a polymer and an inorganic salt in water. It was shown that not only polymers but also ionic liquids in combination with inorganic cosmotrophic salts are able to build ATPS. Suitable for the formation of ionic liquid-based ATPS systems are hydrophilic water miscible ionic liquids. To understand the driving force for amino acid and peptide distribution in IL-ATPS at different pH values, the ionic liquid Ammoeng 110™ and K2 HPO4 have been chosen as a test system...
April 25, 2018: AMB Express
https://www.readbyqxmd.com/read/29696463/salinity-effect-on-the-metabolic-pathway-and-microbial-function-in-phenanthrene-degradation-by-a-halophilic-consortium
#20
Chongyang Wang, Yong Huang, Zuotao Zhang, Hui Wang
With the close relationship between saline environments and industry, polycyclic aromatic hydrocarbons (PAHs) accumulate in saline/hypersaline environments. Therefore, PAHs degradation by halotolerant/halophilic bacteria has received increasing attention. In this study, the metabolic pathway of phenanthrene degradation by halophilic consortium CY-1 was first studied which showed a single upstream pathway initiated by dioxygenation at the C1 and C2 positions, and at several downstream pathways, including the catechol pathway, gentisic acid pathway and protocatechuic acid pathway...
April 25, 2018: AMB Express
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