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AMB Express

Junsei Taira, Rin Toyoshima, Nana Ameku, Akira Iguchi, Yasutomo Tamaki
Vanillin is valuable and popular flavor used in foods and cosmetics. Many bacteria species have the ability to decarboxylate substituted cinnamic acids in order to form vanillin. However, the phenolic biotransformation including vanillin production in a common fungus, the Aspergillus luchuensis, which is used in distilled beverages, has not yet been clarified. This study focused on elucidating the vanillin production due to phenolic biotransformation in A. luchuensis during fermentation. The phenolic metabolites were extracted by a solid phase column and they were determined using on LC/MS and LC/MS/MS in a selective ion mode...
March 13, 2018: AMB Express
Mengzhu Li, Hong Deng, Rui Ma, Huiying Luo, Bin Yao, Xiaoyun Su
Pyranose oxidase (POx) is a homotetrameric flavoprotein that catalyzes the oxidation of pyranose-configured sugars at position C-2 to corresponding 2-ketoaldoses. The wide substrate specificity makes POx potential for application in various biotechnological industries. In the present study we reported the gene cloning and heterologous expression of a POx from the basidiomycete Trametes sp. and functionally expressed the gene in Escherichia coli BL21(DE3). Based on sequence alignment, three residues were chosen for site-directed mutagenesis to obtain two single mutants (K312E and E539K) and two double mutants (T166A/E539K and K312E/E539K)...
March 13, 2018: AMB Express
Yingjie Miao, Guotong Xiong, Ruoyun Li, Zufang Wu, Xin Zhang, Peifang Weng
Issatchenkia orientalis, a non-Saccharomyces yeast that can resist a wide variety of environmental stresses, has potential use in winemaking and bioethanol production. Little is known about gene expression or the physiology of I. orientalis under ethanol stress. In this study, high-throughput RNA sequencing was used to investigate the transcriptome profile of I. orientalis in response to ethanol. 502 gene transcripts were differentially expressed, of which 451 were more abundant, and 51 less abundant, in cells subjected to 4 h of ethanol stress (10% v/v)...
March 13, 2018: AMB Express
Jichen Bao, Mingtao Huang, Dina Petranovic, Jens Nielsen
The yeast Saccharomyces cerevisiae is widely used as a cell factory to produce recombinant proteins. However, S. cerevisiae naturally secretes only a few proteins, such as invertase and the mating alpha factor, and its secretory capacity is limited. It has been reported that engineering protein anterograde trafficking from the endoplasmic reticulum to the Golgi apparatus by the moderate overexpression of SEC16 could increase recombinant protein secretion in S. cerevisiae. In this study, the retrograde trafficking in a strain with moderate overexpression of SEC16 was engineered by overexpression of ADP-ribosylation factor GTP activating proteins, Gcs1p and Glo3p, which are involved in the process of COPI-coated vesicle formation...
March 12, 2018: AMB Express
Imtiaz Hussain Raja Abbasi, Farzana Abbasi, Mohamed E Abd El-Hack, Ayman A Swelum, Junhu Yao, Yangchun Cao
Microbial degradation in the rumen and dietary availability of methionine amino acid have been reported as limiting in dairy ruminants. The aim of the present study was to examine the post-ruminal effects of feeding ruminants different concentrations of rumen-protected methionine (RPM) in low crude protein diets using the long-term rumen simulation method (Rusitec) followed by in vitro abomasum and ileum digestibility technique. The experiment contained four treatment groups: (1) high protein, without RPM supplementation (HP); (2) low protein, without RPM supplementation (LP); (3) low protein supplementation with low RPM (LPLM); and (4) low protein supplementation with high RPM (LPHM) mixed per 20 ± 0...
March 9, 2018: AMB Express
Carolina Díaz-Cárdenas, Angela Cantillo, Laura Yinneth Rojas, Tito Sandoval, Susana Fiorentino, Jorge Robles, Freddy A Ramos, María Mercedes Zambrano, Sandra Baena
The original version of this article (Diaz-Cardenas et al. 2017) unfortunately contained a mistake in Fig. 1. The pie chart of Fig. 1 should explain the distribution of the relative abundance of the Bacteria and Archaea strains isolated at Zipaquirá salt mine: Proteobacteria 39%; Actinobacteria 9%, Bacteroidetes 1%, Archaea 3% and Firmicutes 48% instead of NOMBRE DE CATEGORIA [PORCENTAJE]. The corrected Fig. 1 and caption are given below.
March 9, 2018: AMB Express
Jingwei Xu, Qiang He, Hong Li, Chun Yang, Yinliang Wang, Hainan Ai
Sewer system is an important source of methane formation and emission. Although some models were developed to predict methane production in sewers, the impact of microorganism amount was indicated indirectly. Here, seven laboratory scale sewers with varied wall-shear stresses were established. The biofilm thickness, microorganism amount, DO distribution, microorganism community in the biofilms and methane production in the sewers were measured. Based on experimental data, an empirical model was developed to directly describe the relationship between methane production, microorganism amount and wall-shear stress...
March 7, 2018: AMB Express
Lisa Wasserstrom, Diogo Portugal-Nunes, Henrik Almqvist, Anders G Sandström, Gunnar Lidén, Marie F Gorwa-Grauslund
Engineering of the yeast Saccharomyces cerevisiae towards efficient D-xylose assimilation has been a major focus over the last decades since D-xylose is the second most abundant sugar in nature, and its conversion into products could significantly improve process economy in biomass-based processes. Up to now, two different metabolic routes have been introduced via genetic engineering, consisting of either the isomerization or the oxido-reduction of D-xylose to D-xylulose that is further connected to the pentose phosphate pathway and glycolysis...
March 5, 2018: AMB Express
Krithika Ravi, Javier García-Hidalgo, Matthias Nöbel, Marie F Gorwa-Grauslund, Gunnar Lidén
Bacterial strains were isolated from the sediments of the Baltic Sea using ferulic acid, guaiacol or a lignin-rich softwood waste stream as substrate. In total nine isolates were obtained, five on ferulic acid, two on guaiacol and two on a lignin-rich softwood stream as a carbon source. Three of the isolates were found to be Pseudomonas sp. based on 16S rRNA sequencing. Among them, isolate 9.1, which showed the fastest growth in defined M9 medium, was tentatively identified as a Pseudomonas deceptionensis strain based on the gyrB sequencing...
March 2, 2018: AMB Express
Qian Jiang, Xiwei He, Yuntao Zou, Yin Ding, Huang Li, Huimei Chen
Inflammation has recently been attributed to dysbiosis of the gut microbiome, which has been linked to proteinuria in chronic kidney disease. Since Adriamycin® (ADR) is widely used to induce proteinuria in mouse models, the aim of this study was to explore the potential effect of gut microbiome on this process. Both ADR resistant (C57BL/6) and susceptible (BALB/C) strains were part of the induced nephropathy with ADR injection. BALB/C mice significantly presented increased urinary albumin/creatinine ratio (UACR) with renal lesions in pathology, but C57BL/6 mice were absent from kidney damage...
February 28, 2018: AMB Express
Mandlaa, Ziyu Sun, Ruigang Wang, Xiaodong Han, Hui Xu, Weichao Yang
2-Keto-L-gulonic acid (the precursor of vitamin C) is bio-converted from L-sorbose by mixed fermentation of Ketogulonicigenium vulgare and a helper strain. The helper strain promotes the conversion of 2-KLG by enhancing the growth of K. vulgare, but its growth is greatly inhibited by high concentration of L-sorbose, which consequently influence the 2-KLG production. The aim of this study is to obtain L-sorbose-tolerant helper strain (LHS) by experimental evolution for reduced L-sorbose-inhibition-effect and enhanced 2-KLG productivity in high concentration of L-sorbose...
February 28, 2018: AMB Express
Biao Chen, Liu-Ling Gao, Qing Pan
To understand the effect of woody forages on golden pompano (Trachinotus ovatus) intestinal bacteria diversity and exploit new aquafeed ingredients, the diets of Moringa oleifera Lam (MOL), Broussonetia papyrifera (BP), Neolamarckia cadamba (NC) and Folium mori (FM) formulated with 70% of reference (Ref) diet and 30% of the four woody plants leaves were fed to golden pompano with initial body weight of 34.4 ± 0.5 g for 56 days, respectively. Bacteria diversity of golden pompano intestine and tank water (W) samples were analyzed using high-throughput illumina sequencing and the result indicated that the dominate phyla of golden pompano intestine were Proteobacteria, Bacteroidetes, Firmicutes and Fusobacteria...
February 27, 2018: AMB Express
Bin Zhang, Miao Yu, Ying Zhou, Bang-Ce Ye
L-Ornithine, a non-essential amino acid, has enormous industrial applications in food, pharmaceutical, and chemical industries. Currently, L-ornithine production is focused on microorganism fermentation using Escherichia coli or Corynebacterium glutamicum. In C. glutamicum, development of high L-ornithine producing C. glutamicum was achieved by deletion of argF, but was accompanied by growth deficiency and arginine auxotrophy. L-Arginine has been routinely added to solve this problem; however, this increases production cost and causes feedback inhibition of N-acetyl-L-glutamate kinase activity...
February 24, 2018: AMB Express
Zhineng Wu, Miaomiao Xie, Yao Li, Guanghai Gao, Mark Bartlam, Yingying Wang
Polybrominated diphenyl ethers (PBDEs) have become widespread environmental pollutants all over the world. A newly isolated bacterium from an e-waste recycling area, Stenotrophomonas sp. strain WZN-1, can degrade decabromodiphenyl ether (BDE 209) effectively under aerobic conditions. Orthogonal test results showed that the optimum conditions for BDE 209 biodegradation were pH 5, 25 °C, 0.5% salinity, 150 mL minimal salt medium volume. Under the optimized condition, strain WZN-1 could degrade 55.15% of 65 μg/L BDE 209 under aerobic condition within 30 day incubation...
February 24, 2018: AMB Express
Veronika Hahn, Mareike Meister, Stephan Hussy, Arno Cordes, Günther Enderle, Akuma Saningong, Frieder Schauer
The inadequate removal of pharmaceuticals and other micropollutants in municipal wastewater treatment plants, as evidenced by their detection of these substances in the aquatic environment has led to the need for sustainable remediation strategies. Laccases possess a number of advantages including a broad substrate spectrum. To identify promoting or inhibitory effects of reaction partners in the remediation processes we tested not only single compounds-as has been described in most studies-but also mixtures of pollutants...
February 24, 2018: AMB Express
Mohammad Anwar Siddique, Md Inja-Mamun Haque, Santonu Kumar Sanyal, Anwar Hossain, Shuvro Prokash Nandi, A S M Rubayet Ul Alam, Munawar Sultana, Mahmud Hasan, M Anwar Hossain
White Spot Syndrome Virus (WSSV), the etiological agent of White Spot Disease (WSD) is a major impediment for shrimp aquaculture in the worldwide. A critical threshold level of WSSV load in infected shrimp is an important trait for disease manifestation and WSSV transmission in cultured shrimp and subsequently make outbreaks. The present study investigated 120 naturally infected cultured shrimp samples by SYBR Green based qPCR assay for WSD diagnosis and quantification of WSSV load. Among them, 94 samples resulted a variable count of WSSV load ranging from 2...
February 20, 2018: AMB Express
Hans Gollwitzer, Maximilian Haenle, Wolfram Mittelmeier, Frank Heidenau, Norbert Harrasser
Implant-associated infections are dangerous complications and may cause dramatic illness with hematogeneous spread of bacteria and secondary infections. Since treatment of these infections remains most challenging and commonly requires implant removal, prevention is of utmost importance. In the present work a titania-sol was equipped with a copper salt resulting after calcination in a titania coating (TiO2 ) with antibacterial properties combined with good cytocompatibility. In vitro tests with bacteria as well as tissue cells were carried out under corresponding conditions...
February 19, 2018: AMB Express
Hang Ren, Ya-Ting Su, Xiao-Hua Guo
Some optimization techniques have been widely applied for spore fermentation based on the plate counting. This study optimized the culture medium for the spore production of Bacillus amyloliquefaciens BS-20 and investigated the feasibility of using a dipicolonic acid (DPA) fluorimetry assay as a simpler alternative to plate counting for evaluating spore yields. Through the single-factor experiment, the metal ions and agro-industrial raw materials that significantly enhanced spore production were determined...
February 16, 2018: AMB Express
Xianlei Cai, Ling Yao, Qiyue Sheng, Luyao Jiang, Randy A Dahlgren, Ting Wang
Bacterial communities of biofilms growing on artificial substrates were examined at two time periods (7 and 14 days) and two locations (lentic and lotic areas) in a hypereutrophic urban river of eastern China. Previous studies in this river network indicated that variations of microbial communities were the major factor affecting the distribution of antibiotic resistant genes highlighting the importance of understanding controls of microbial communities. Bacterial communities associated with biofilms were determined using epifluorescence microscopy and high-throughput sequencing...
February 16, 2018: AMB Express
Vanda R Reis, Ana Paula G Bassi, Bianca C Cerri, Amanda R Almeida, Isis G B Carvalho, Reinaldo G Bastos, Sandra R Ceccato-Antonini
Even though contamination by bacteria and wild yeasts are frequently observed during fuel ethanol fermentation, our knowledge regarding the effects of both contaminants together is very limited, especially considering that the must composition can vary from exclusively sugarcane juice to a mixture of molasses and juice, affecting the microbial development. Here we studied the effects of the feedstock (sugarcane juice and molasses) and the co-culture of Lactobacillus fermentum and a wild Saccharomyces cerevisiae strain (rough colony and pseudohyphae) in single and multiple-batch fermentation trials with an industrial strain of S...
February 16, 2018: AMB Express
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