journal
MENU ▼
Read by QxMD icon Read
search

AMB Express

journal
https://www.readbyqxmd.com/read/28549374/metabolic-engineering-of-escherichia-coli-to-high-efficient-synthesis-phenylacetic-acid-from-phenylalanine
#1
Lihua Zhang, Qian Liu, Hong Pan, Xun Li, Daoyi Guo
Phenylacetic acid (PAA) is a fine chemical with a high industrial demand for its widespread uses. Whereas, microorganic synthesis of PAA is impeded by the formation of by-product phenethyl alcohol due to quick, endogenous, and superfluous conversion of aldehydes to their corresponding alcohols, which resulted in less conversation of PAA from aldehydes. In this study, an Escherichia coli K-12 MG1655 strain with reduced aromatic aldehyde reduction (RARE) that does duty for a platform for aromatic aldehyde biosynthesis was used to prompt more PAA biosynthesis...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28549373/production-of-a-potential-liquid-plant-bio-stimulant-by-immobilized-piriformospora-indica-in-repeated-batch-fermentation-process
#2
Nikolay Vassilev, Bettina Eichler-Löbermann, Elena Flor-Peregrin, Vanessa Martos, Antonia Reyes, Maria Vassileva
Piriformospora indica, a mycorrhizal-like fungus able to establish associations with roots of a wide range of plants, supporting plant nutrition and increasing plant resistance and tolerance to stress, was shown to solubilise phosphate applied in the form of animal bone char (HABO) in fermentation systems. The process of P solubilisation was caused most likely by proton extrusion and medium pH lowering. The fungal mycelium was successfully immobilized/retained in a polyurethane foam carrier. Further employment of the immobilized mycelium in repeated-batch fermentation process resulted in at least 5 cycles of P solubilization...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28549372/pgpr-strain-paenibacillus-polymyxa-sqr-21-potentially-benefits-watermelon-growth-by-re-shaping-root-protein-expression
#3
Yaoyao E, Jun Yuan, Fang Yang, Lei Wang, Jinghua Ma, Jing Li, Xiaowei Pu, Waseem Raza, Qiwei Huang, Qirong Shen
Paenibacillus polymyxa (SQR-21) is not only a plant growth-promoting rhizobacteria, but also an effective biocontrol agent against Fusarium wilt disease of watermelon. For the better understanding and clarifying the potential mechanisms of SQR-21 to improve watermelon growth and disease resistance, a split-root methodology in hydroponic and LC-MS technology with the label free method was used to analyze the key root proteins involved in watermelon metabolism and disease resistance after the inoculation of SQR-21...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28545261/microbicidal-activity-of-n-chlorotaurine-in-combination-with-hydrogen-peroxide
#4
Jasmin Mustedanagic, Valdecir Farias Ximenes, Markus Nagl
N-chlorotaurine (NCT) and hydrogen peroxide are powerful endogenous antiseptics. In vivo, the reaction between hydrogen peroxide and metal ions leads to the formation of free hydroxyl radicals, which have an increased bactericidal activity. This study examined whether there is an additive antimicrobial effect of NCT combined with hydrogen peroxide. Additionally, it was tested if the additive effect is based on the formation of free radicals. We found by luminometry that, in the presence of H2O2, NCT caused a slow and long-lasting production of singlet oxygen in contrast to HOCl, where this burst occurred instantaneously...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28545260/thermostabilization-of-the-uronate-dehydrogenase-from-agrobacterium-tumefaciens-by-semi-rational-design
#5
Teresa Roth, Barbara Beer, André Pick, Volker Sieber
Aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. Until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. An environmentally friendly and more selective process could be achieved by using natural resources such as seaweed or pectin as raw material. These contain large amounts of uronic acids as major constituents such as glucuronic acid and galacturonic acid which can be converted into the corresponding aldaric acids via an enzyme-based oxidation using uronate dehydrogenase (Udh)...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28545259/identification-of-diacetonamine-from-soybean-curd-residue-as-a-sporulation-inducing-factor-toward-bacillus-spp
#6
Aki Ikeda, Dongyeop Kim, Yasuyuki Hashidoko
Under bioassay-guided investigation, a sporulation-inducing factor (SIF) toward Bacillus spp. was searched for in methanol (MeOH) extracts of soybean curd residues, and diacetonamine (1) was identified as the active compound. SIF was first isolated as a monoacetylated derivative (2, 4.1 mg from 655 g soybean curd residues), and its chemical structure was elucidated by field desorption mass spectrometry, electron ionization mass spectrometry, and nuclear magnetic resonance (NMR) analyses. After 48-h incubation, 40 µM diacetonamine hydrochloride (1b) exhibited sporulation-inducing activity with 35% sporulation frequency toward a Bacillus amyloliquefaciens wild-type strain (AHU 2170), whereas 40 µM diacetone acrylamide (3) showed 99% sporulation induction, which was much higher than that of 1b...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28532122/mannan-endo-1-4-%C3%AE-mannosidase-from-kitasatospora-sp-isolated-in-indonesia-and-its-potential-for-production-of-mannooligosaccharides-from-mannan-polymers
#7
Nanik Rahmani, Norimasa Kashiwagi, JaeMin Lee, Satoko Niimi-Nakamura, Hana Matsumoto, Prihardi Kahar, Puspita Lisdiyanti, Yopi, Bambang Prasetya, Chiaki Ogino, Akihiko Kondo
Mannan endo-1,4-β-mannosidase (commonly known as β-mannanase) catalyzes a random cleavage of the β-D-1,4-mannopyranosyl linkage in mannan polymers. The enzyme has been utilized in biofuel production from lignocellulose biomass, as well as in production of mannooligosaccharides (MOS) for applications in feed and food industries. We aimed to obtain a β-mannanase, for such mannan polymer utilization, from actinomycetes strains isolated in Indonesia. Strains exhibiting high mannanase activity were screened, and one strain belonging to the genus Kitasatospora was selected...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28532121/antimicrobial-activity-of-puffball-bovistella-radicata-and-separation-of-bioactive-compounds
#8
Yong Ye, Kun Liu, Qinghua Zeng, Qingmei Zeng
BACKGROUND: To test the antimicrobial activity of different extracts and fermentation broth from puffball(Bovistella radicata), the different extracts and fermentation broth of puffball were prepared, the active fraction was investigated by UPLC-UV-MS and semi-preparative chromatograph. RESULTS: Through zones of inhibition (ZOI) and minimum inhibitory concentrations (MIC) tests, the supernatant of fermentation possessed best antimicrobial activity in all extracts whose MIC value is 31...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28510221/development-of-a-dual-expression-vector-facilitated-with-selection-free-pcr-recombination-cloning-strategy
#9
Liting Cao, Yancheng Zhou, Lin Huang, Shiqi Dong, Yue Ma
The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR cloning strategies have been developed, but they either involve complicated PCR procedures or need other DNA modifying enzymes. In this study, we report the design, and construction of an omnipotent expression vector pOmni, with which a target gene can be easily cloned through innovative selection-free PCR recombination cloning strategy with only one pair of primer and two times of PCR in one work day, without using any restriction enzymes, ligase and other DNA modifying enzymes...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28508386/application-of-response-surface-methodology-for-optimization-of-medium-components-for-the-production-of-secondary-metabolites-by-streptomyces-diastatochromogenes-kx852460
#10
Taswar Ahsan, Jianguang Chen, Yuanhua Wu, Muhammad Irfan
A bioactive strain Streptomyces diastatochromogenes KX852460 was selected for the production of secondary metabolites to control the target spot disease on tobacco leaves, caused by the Rhizoctonia solani AG-3. Peanut meal, soluble starch, NaCl, yeast extract, and ammonium sulphate were identified the best ingredient for high antifungal activity of S. diastatochromogenes KX852460 against the R. solani AG-3. For the improved production of secondary metabolites, central composite design of response surface methodology was applied in submerged fermentation...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28508385/improving-the-temperature-characteristics-and-catalytic-efficiency-of-a-mesophilic-xylanase-from-aspergillus-oryzae-aoxyn11a-by-iterative-mutagenesis-based-on-in-silico-design
#11
Xue-Qing Li, Qin Wu, Die Hu, Rui Wang, Yan Liu, Min-Chen Wu, Jian-Fang Li
To improve the temperature characteristics and catalytic efficiency of a glycoside hydrolase family (GHF) 11 xylanase from Aspergillus oryzae (AoXyn11A), its variants were predicted based on in silico design. Firstly, Gly(21) with the maximum B-factor value, which was confirmed by molecular dynamics (MD) simulation on the three-dimensional structure of AoXyn11A, was subjected to site-saturation mutagenesis. Thus, one variant with the highest thermostability, AoXyn11A(G21I), was selected from the mutagenesis library, E...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28500590/autolysis-of-pichia-pastoris-induced-by-cold
#12
Yaneth Bartolo-Aguilar, Luc Dendooven, Cipriano Chávez-Cabrera, Luis B Flores-Cotera, María E Hidalgo-Lara, Lourdes Villa-Tanaca, Rodolfo Marsch
The production of recombinant biopharmaceutical proteins is a multi-billion dollar market. Protein recovery represents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for the production of heterologous proteins. The use of a cold-induced promoter to express lytic enzymes in the yeast after the growth stage could reduce protein recovery costs. This study shows that a cold-shock can be applied to induce lysis of the yeast cells. A strain of P. pastoris was constructed in which the endogenous eng gene encoding a putative endo-β-1,3-glucanase was overexpressed using the cold-shock induced promoter of the cctα gene from Saccharomyces cerevisiae...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28497290/medium-chain-length-polyhydroxyalkanoates-synthesis-by-pseudomonas-putida-kt2440-rela-spot-mutant-bioprocess-characterization-and-transcriptome-analysis
#13
Justyna Mozejko-Ciesielska, Dorota Dabrowska, Agnieszka Szalewska-Palasz, Slawomir Ciesielski
Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli are still not clear. The rearrangement of the metabolism in response to environmental stress could be controlled by stringent response that modulates the transcription of many genes in order to promote survival under nutritional deprivation conditions...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28497289/systematic-analysis-of-the-lysine-acetylome-reveals-diverse-functions-of-lysine-acetylation-in-the-oleaginous-yeast-yarrowia-lipolytica
#14
Guangyuan Wang, Lizhong Guo, Wenxing Liang, Zhenming Chi, Lin Liu
Lysine acetylation of proteins, a major post-translational modification, plays a critical regulatory role in almost every aspects in both eukaryotes and prokaryotes. Yarrowia lipolytica, an oleaginous yeast, is considered as a model for bio-oil production due to its ability to accumulate a large amount of lipids. However, the function of lysine acetylation in this organism is elusive. Here, we performed a global acetylproteome analysis of Y. lipolytica ACA-DC 50109. In total, 3163 lysine acetylation sites were identified in 1428 proteins, which account for 22...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28497288/novel-signal-peptides-improve-the-secretion-of-recombinant-staphylococcus-aureus-alpha-toxinh35l-in-escherichia-coli
#15
SooJin Han, Shushil Machhi, Mark Berge, Guoling Xi, Thomas Linke, Ronald Schoner
Secretion of heterologous proteins into Escherichia coli cell culture medium offers significant advantages for downstream processing over production as inclusion bodies; including cost and time savings, and reduction of endotoxin. Signal peptides play an important role in targeting proteins for translocation across the cytoplasmic membrane to the periplasmic space and release into culture medium during the secretion process. Alpha toxinH35L (ATH35L) was selected as an antigen for vaccine development against Staphylococcus aureus infections...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28497287/role-of-nitrite-in-the-competition-between-denitrification-and-dnra-in-a-chemostat-enrichment-culture
#16
Eveline M van den Berg, Julius L Rombouts, J Gijs Kuenen, Robbert Kleerebezem, Mark C M van Loosdrecht
Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes that compete for oxidized nitrogen compounds in the environment. The objective of this work was to determine the role of nitrite versus nitrate as terminal electron acceptor on the competition between DNRA and denitrification. Initially, a mixed culture chemostat was operated under nitrate limitation and performed DNRA. Stepwise, the influent nitrate was replaced with nitrite until nitrite was the sole electron acceptor and N-source present...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28488255/cysteine-degradation-gene-yham-encoding-cysteine-desulfidase-serves-as-a-genetic-engineering-target-to-improve-cysteine-production-in-escherichia-coli
#17
Gen Nonaka, Kazuhiro Takumi
Cysteine is an important amino acid for various industries; however, there is no efficient microbial fermentation-based production method available. Owing to its cytotoxicity, bacterial intracellular levels of cysteine are stringently controlled via several modes of regulation, including cysteine degradation by cysteine desulfhydrases and cysteine desulfidases. In Escherichia coli, several metabolic enzymes are known to exhibit cysteine degradative activities, however, their specificity and physiological significance for cysteine detoxification via degradation are unclear...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28484998/enhanced-methane-production-of-vinegar-residue-by-response-surface-methodology-rsm
#18
Jiayu Feng, Jiyu Zhang, Jiafu Zhang, Yanfeng He, Ruihong Zhang, Chang Chen, Guangqing Liu
As the by-product of the vinegar production process, a large number of vinegar residue has been abandoned and caused a serious environmental pollution. Anaerobic digestion has been proved to be able to dispose and convert vinegar residue into bioenergy but still need to improve the efficiency. This study applied central composite design of response surface methodology to investigate the influences of feed to inoculum ratio, organic loading, and initial pH on methane production and optimize anaerobic digestion condition...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28452041/cloning-and-characterization-of-the-type-i-baeyer-villiger-monooxygenase-from-leptospira-biflexa
#19
Romina D Ceccoli, Dario A Bianchi, Michael J Fink, Marko D Mihovilovic, Daniela V Rial
Baeyer-Villiger monooxygenases are recognized by their ability and high selectivity as oxidative biocatalysts for the generation of esters or lactones using ketones as starting materials. These enzymes represent valuable tools for biooxidative syntheses since they can catalyze reactions that otherwise involve strong oxidative reagents. In this work, we present a novel enzyme, the Type I Baeyer-Villiger monooxygenase from Leptospira biflexa. This protein is phylogenetically distant from other well-characterized BVMOs...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28452040/biocatalytic-activity-of-monascus-mycelia-depending-on-physiology-and-high-sensitivity-to-product-concentration
#20
Fengling Lu, Yaolin Huang, Xuehong Zhang, Zhilong Wang
Cell suspension culture using mycelia as whole cell biocatalyst for production of orange Monascus pigments has been carried out successfully in a nonionic surfactant micelle aqueous solution. Thus, selection of mycelia as whole cell biocatalyst and the corresponding enzymatic kinetics for production of orange Monascus pigments can be optimized independently. Mycelia selected from submerged culture in a nonionic surfactant micelle aqueous solution with low pH 2.5 exhibits robust bioactivity. At the same time, enzymatic kinetic study shows that the bioactivity of mycelia as whole cell biocatalyst is sensitive to high product concentration...
December 2017: AMB Express
journal
journal
43602
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"