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AMB Express

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https://www.readbyqxmd.com/read/28439850/heterologous-expression-of-a-streptomyces-cyaneus-laccase-for-biomass-modification-applications
#1
Selin Ece, Camilla Lambertz, Rainer Fischer, Ulrich Commandeur
Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L(-1) following purification by one-step affinity chromatography. Stability and activity assays using simple lignin model substrates showed that the purified enzyme preparation was active over a broad pH range and at high temperatures, suggesting it would be suitable for biomass degradation...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28429330/growth-kinetics-fatty-acid-composition-and-metabolic-activity-changes-of-crypthecodinium-cohnii-under-different-nitrogen-source-and-concentration
#2
Waseem Safdar, Muhammad Shamoon, Xinyi Zan, Junaid Haider, Hafiz Rizwan Sharif, Muhammad Shoaib, Yuanda Song
The effect of varying concentrations of the nitrogen source on the growth kinetics, lipid accumulation, lipid and DHA productivity, and fatty acid composition of C. cohnii was elucidated. Growth of C. cohnii was in three distinct growth stages: cell growth, lipid accumulation and a final lipid turnover stage. Most of lipids were accumulated in lipid accumulation stage (48-120 h) though, slow growth rate was observed during this stage. NaNO3 supported significantly higher lipid content (26.9% of DCW), DHA content (0...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28429329/hydrogen-production-and-microbial-kinetics-of-clostridium-termitidis-in-mono-culture-and-co-culture-with-clostridium-beijerinckii-on-cellulose
#3
Maritza Gomez-Flores, George Nakhla, Hisham Hafez
Cellulose utilization by hydrogen producers remains an issue due to the low hydrogen yields reported and the pretreatment of cellulose prior to fermentation requires complex and expensive steps. Clostridium termitidis is able to breakdown cellulose into glucose and produce hydrogen. On the other hand, Clostridium beijerinckii is not able to degrade cellulose but is adept at hydrogen production from glucose; therefore, it was chosen to potentially enhance hydrogen production when co-cultured with C. termitidis on cellulose...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28429328/ammonia-production-from-amino-acid-based-biomass-like-sources-by-engineered-escherichia-coli
#4
Yosuke Mikami, Hisanari Yoneda, Yohei Tatsukami, Wataru Aoki, Mitsuyoshi Ueda
The demand for ammonia is expected to increase in the future because of its importance in agriculture, industry, and hydrogen transportation. Although the Haber-Bosch process is known as an effective way to produce ammonia, the process is energy-intensive. Thus, an environmentally friendly ammonia production process is desired. In this study, we aimed to produce ammonia from amino acids and amino acid-based biomass-like resources by modifying the metabolism of Escherichia coli. By engineering metabolic flux to promote ammonia production using the overexpression of the ketoisovalerate decarboxylase gene (kivd), derived from Lactococcus lactis, ammonia production from amino acids was 351 mg/L (36...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28417435/lachnospiraceae-shift-in-the-microbial-community-of-mice-faecal-sample-effects-on-water-immersion-restraint-stress
#5
Shiyin Li, Zelin Wang, Yun Yang, Sha Yang, Chenchen Yao, Kaiyun Liu, Sixin Cui, Quanming Zou, Hongwu Sun, Gang Guo
Stress, including both psychological and physical stimulation, can cause changes in the microbiota and mucosal function of the gastrointestinal system. There are few research studies available about the faecal microbiota changes after stress, such as water immersion restraint stress (WIRS). Therefore, in this study, we focused on analysing the composition changes of faecal microbiota in WIRS mice. The WIRS model, in which Blab/c mice were immersed in 21 ± 2 °C water for 4 h each day for 14 days, was established...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28413853/characterization-of-the-gut-microbiota-in-the-golden-takin-budorcas-taxicolor-bedfordi
#6
Jun Chen, Huanxin Zhang, Xiaoyang Wu, Shuai Shang, Jiakuo Yan, Yao Chen, Honghai Zhang, Xuexi Tang
The gut microbiota of mammals is a complex ecosystem, which is essential for maintaining gut homeostasis and the host's health. The high throughput sequencing allowed us to gain a deeper insight into the bacterial structure and diversity. In order to improve the health status of the endangered golden takins, we first characterized the fecal microbiota of healthy golden takins using high throughput sequencing of the 16S rRNA genes V3-V4 hypervariable regions. Our results showed that, Firstly, the gut microbiota community comprised 21 phyla, 40 classes, 62 orders, 96 families, and 216 genera...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28411348/quantitative-detection-of-fecal-contamination-with-domestic-poultry-feces-in-environments-in-china
#7
Fang-Fang Zhuang, Hu Li, Xin-Yuan Zhou, Yong-Guan Zhu, Jian-Qiang Su
Poultry are an important source of fecal contamination in environments. However, tools for detecting and tracking this fecal contamination are in the early stages of development. In practice, we have found that source tracking methods targeting the 16S rRNA genes of poultry-specific microbiota are not sufficiently sensitive. We therefore developed two quantitative PCR assays for detection of poultry fecal contamination, by targeting chicken and duck mitochondrial genes: NADH dehydrogenase subunit 5 (ND5) and cytochrome b (cytb)...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28401504/metabolism-and-secretion-of-yellow-pigment-under-high-glucose-stress-with-monascus-ruber
#8
Tao Huang, Meihua Wang, Kan Shi, Gong Chen, Xiaofei Tian, Zhenqiang Wu
The biosynthesis of microbial secondary metabolites is induced by a wide range of environmental stresses. In this study, submerged fermentation of Monascus yellow pigments by Monascus ruber CGMCC 10910 under high glucose stress was investigated. The increase of lipid content was the major contributor to the increase of dry cell weight (DCW), and the lipid-free DCW was only slightly changed under high glucose stress, which benefited the accumulation of intracellular hydrophobic pigments. The fatty acid composition analysis in Monascus cell membranes showed that high glucose stress significantly increased the ratio of unsaturated/saturated fatty acid and the index of unsaturated fatty acid (IUFA) value, which would improve the fluidity and permeability of the cell membrane...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28391484/genomic-and-molecular-characterization-of-a-novel-quorum-sensing-molecule-in-bacillus-licheniformis
#9
Elham Esmaeilishirazifard, Daniela De Vizio, Sterghios A Moschos, Tajalli Keshavarz
Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been identified in the genome sequence of some bacilli, the QS system B. licheniformis has not been investigated in detail, and its QSM (ComX pheromone) has not been identified. Given the importance of this antagonistic bacterium as an industrial workhorse, this study was aimed to elucidate B. licheniformis NCIMB-8874 QS...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28389899/degradation-and-inactivation-of-shiga-toxins-by-nitrogen-gas-plasma
#10
Akikazu Sakudo, Yuichiro Imanishi
Shiga toxin (Stx)-producing Escherichia coli (STEC) leads to food poisoning by causing hemorrhagic colitis and hemolytic uremic syndrome. Some STEC produce Shiga toxin 1 (Stx1) and/or Shiga toxin 2 (Stx2), a relatively stable protein toxin, necessitating the development of an efficient inactivation method. Here we applied a nitrogen gas plasma apparatus to the inactivation of Stx. Samples of Stx1 and Stx2 were treated with a nitrogen gas plasma generated by a plasma device using a short high-voltage pulse applied by a static induction thyristor power supply at 1...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28378285/nitrate-decreases-methane-production-also-by-increasing-methane-oxidation-through-stimulating-nc10-population-in-ruminal-culture
#11
Lihui Liu, Xiurong Xu, Yangchun Cao, Chuanjiang Cai, Hongxiao Cui, Junhu Yao
Studies proved that addition of nitrate in rumen could lead to reduction of methane emission. The mechanism of this function was involved in the competition effect of nitrate on hydrogen consumption and the inhibitory effect of generated nitrite on methanogen proliferation. The present study investigated an alternative mechanism that denitrifying anaerobic methane oxidizing (DAMO) bacteria, DAMO archaea and anammox bacteria may co-exist in rumen, therefore, more methane can be oxidized when addition of nitrate...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28378284/microbial-community-compositions-in-the-gastrointestinal-tract-of-chinese-mongolian-sheep-using-illumina-miseq-sequencing-revealed-high-microbial-diversity
#12
Yan Zeng, Dong Zeng, Xueqin Ni, Hui Zhu, Ping Jian, Yi Zhou, Shuai Xu, Yicen Lin, Yang Li, Zhongqiong Yin, Kangcheng Pan, Bo Jing
Chinese Mongolian sheep are an important ruminant raised for wool and meat production. However, little is known about the microbiota of the gastrointestinal tract (GIT) of Chinese Mongolian sheep. To increase our understanding of the microbial community composition in the GIT of Chinese Mongolian sheep, microbiota of five sheep is investigate for the first time using the Illumina MiSeq platform. High microbial diversity was obtained from the GIT, and the microbiota exhibited a higher biodiversity in the stomach and large intestine than in the small intestine...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28374344/screening-of-alginate-lyase-excreting-microorganisms-from-the-surface-of-brown-algae
#13
Mingpeng Wang, Lei Chen, Zhaojie Zhang, Xuejiang Wang, Song Qin, Peisheng Yan
Alginate lyase is a biocatalyst that degrades alginate to produce oligosaccharides, which have many bioactive functions and could be used as renewable biofuels. Here we report a simple and sensitive plate assay for screening alginate lyase-excreting microorganisms from brown algae. Brown algae Laminaria japonica, Sargassum horneri and Sargassum siliquatrum were cultured in sterile water. Bacteria growing on the surface of seaweeds were identified and their capacity of excreting alginate lyase was analyzed. A total of 196 strains were recovered from the three different algae samples and 12 different bacterial strains were identified capable of excreting alginate lyases...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28357784/expression-of-a-new-laccase-from-moniliophthora-roreri-at-high-levels-in-pichia-pastoris-and-its-potential-application-in-micropollutant-degradation
#14
Agathe Bronikowski, Peter-Leon Hagedoorn, Katja Koschorreck, Vlada B Urlacher
Laccases have gained significant attention due to their emerging applications including bioremediation, biomass degradation and biofuel cells. One of the prerequisites for the industrial application of laccases is their sufficient availability. However, expression levels of recombinantly expressed laccases are often low. In this study Mrl2, a new laccase from the basidiomycete Moniliophthora roreri, was cloned in Pichia pastoris and produced in an optimized fed-batch process at an exceptionally high yield of 1...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28353158/pretreatment-methods-of-lignocellulosic-biomass-for-anaerobic-digestion
#15
REVIEW
Farrukh Raza Amin, Habiba Khalid, Han Zhang, Sajid U Rahman, Ruihong Zhang, Guangqing Liu, Chang Chen
Agricultural residues, such as lignocellulosic materials (LM), are the most attractive renewable bioenergy sources and are abundantly found in nature. Anaerobic digestion has been extensively studied for the effective utilization of LM for biogas production. Experimental investigation of physiochemical changes that occur during pretreatment is needed for developing mechanistic and effective models that can be employed for the rational design of pretreatment processes. Various-cutting edge pretreatment technologies (physical, chemical and biological) are being tested on the pilot scale...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28342171/expressing-anti-hiv-vrc01-antibody-using-the-murine-igg1-secretion-signal-in-pichia-pastoris
#16
Rochelle Aw, Paul F McKay, Robin J Shattock, Karen M Polizzi
The use of the recombinant expression platform Pichia pastoris to produce pharmaceutically important proteins has been investigated over the past 30 years. Compared to mammalian cultures, expression in P. pastoris is cheaper and faster, potentially leading to decreased costs and process development times. Product yields depend on a number of factors including the secretion signal chosen for expression, which can influence the host cell response to recombinant protein production. VRC01, a broadly neutralising anti-HIV antibody, was expressed in P...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28342170/importance-of-stress-response-genes-to-the-survival-of-airborne-escherichia-coli-under-different-levels-of-relative-humidity
#17
Tsz Wai Ng, Wing Lam Chan, Ka Man Lai
Other than the needs for infection control to investigate the survival and inactivation of airborne bacterial pathogens, there has been a growing interest in exploring bacterial communities in the air and the effect of environmental variables on them. However, the innate biological mechanism influencing the bacterial viability is still unclear. In this study, a mutant-based approach, using Escherichia coli as a model, was used to prove the concept that common stress-response genes are important for airborne survival of bacteria...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28337735/isolation-of-nontuberculous-mycobacteria-from-soil-using-middlebrook-7h10-agar-with-increased-malachite-green-concentration
#18
Yuli Hu, Xinglong Yu, Dun Zhao, Runcheng Li, Yang Liu, Meng Ge, Huican Hu
Environmental exposure is considered to be responsible for nontuberculous mycobacterial infections in humans. To facilitate the isolation of mycobacteria from soil, Middlebrook 7H10 agar was optimized as an enhanced selective medium by increasing the concentration of malachite green. A series of modified Middlebrook 7H10 agar media with malachite green concentrations ranging from 2.5 to 2500 mg/L was evaluated using 20 soil samples decontaminated with 3% sodium dodecyl sulfate plus 2% NaOH for 30 min. Among these modified Middlebrook 7H10 media, the medium with malachite green at a concentration of 250 mg/L, i...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28324616/optimization-of-diazinon-biodegradation-from-aqueous-solutions-by-saccharomyces-cerevisiae-using-response-surface-methodology
#19
Mohammad H Ehrampoush, Abbas Sadeghi, Mohammad T Ghaneian, Ziaeddin Bonyadi
Diazinon is an organophosphate compound that inhibits the activity of acetylcholinesterase. Standards of the World Health Organization and Environmental Protection Agency for diazinon concentration in water are 0.1 and 9 × 10(-6) mg/L, respectively. The aim of this study was the optimization of diazinon biodegradation from aqueous solutions by Saccharomyces cerevisiae using the response surface methodology (RSM). Harvested cells of S. cerevisiae were locally purchased from the Iranian Research Organization for Science and Technology...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28324615/construction-of-low-ethanol-wine-yeasts-through-partial-deletion-of-the-saccharomyces-cerevisiae-pdc2-gene
#20
Raúl Andrés Cuello, Karina Johana Flores Montero, Laura Analía Mercado, Mariana Combina, Iván Francisco Ciklic
We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through homologous recombination, two truncated Pdc2p proteins Pdc2pΔ344 and Pdc2pΔ519 were obtained and transformed into haploid and diploid lab yeast strains. In the pdc2Δ344 mutants the DNA-binding and transactivation site of the protein remain intact, whereas in pdc2Δ519 only the DNA-binding site is conserved...
December 2017: AMB Express
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