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Cell & Bioscience

Liang Wang, Jingyan Shen, Yushan Jiang
Background: Pleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs (circRNAs) is not known. CircRNAs are important regulators in cancer growth and progression, however, the molecular roles of circRNAs in gastric cancer are rarely known. The study was aimed to investigate the role of circRNAs in regulating PHLDA1 expression in gastric cancer. Results: The circRNA expression profile in the gastric cancer tissues by circRNA microarray showed that hsa_circ_0027599 (circ_0027599) was significantly down-regulated in gastric cancer patients and cells when comparing with the controls...
2018: Cell & Bioscience
Sarada Preeta Kalainayakan, Keely E FitzGerald, Purna Chaitanya Konduri, Chantal Vidal, Li Zhang
Contrary to Warburg's hypothesis, mitochondrial oxidative phosphorylation (OXPHOS) contributes significantly to fueling cancer cells. Several recent studies have demonstrated that radiotherapy-resistant and chemotherapy-resistant cancer cells depend on OXPHOS for survival and progression. Several cancers exhibit an increased risk in association with heme intake. Mitochondria are widely known to carry out oxidative phosphorylation. In addition, mitochondria are also involved in heme synthesis. Heme serves as a prosthetic group for several proteins that constitute the complexes of mitochondrial electron transport chain...
2018: Cell & Bioscience
Fei Liu, Zhifeng Wang, Fangfang Liu, Jinzhao Xu, Qibo Liu, Kaifeng Yin, Jing Lan
Background: Fine osseointegration is the basis of long-term survival of implant. In our previous study, we observed a strong correlation between hyperlipidemia and compromised osseointegration. MicroRNA-29a-3p (miR-29a-3p) has been discovered to participate in bone marrow mesenchymal stem cells (BMSCs) differentiation. However, the role and the underlying mechanisms of hyperlipidemia and miR-29a-3p in osseointegration still remain obscure. Results: In peri-implant bone tissues of hyperlipidemia rats, bone mass, mineralization and bone trabecula formation were weakened...
2018: Cell & Bioscience
Winifred Mak, Jing Xia, Ee-Chun Cheng, Katie Lowther, Haifan Lin
Background: RNA binding proteins play a pivotal role during the oocyte-to-embryo transition and maternal phase of embryogenesis in invertebrates, but their function in these processes in mammalian systems remain largely understudied. Results: Here we report that a member of the Pumilio/FBF family of RNA binding proteins in mice, Pumilio 1 ( Pum 1), is a maternal effect gene. The absence of maternal PUM1 in the oocyte does not affect meiotic maturation but leads to abnormal preimplantation development...
2018: Cell & Bioscience
Praveen Barrodia, Chinmoy Patra, Rajeeb K Swain
Background: The blood filtering organ in zebrafish embryos is the pronephros, which consists of two functional nephrons. Segmentation of a nephron into different domains is essential for its function and is well conserved among vertebrates. Zebrafish has been extensively used as a model to understand nephron segmentation during development. Here, we have identified EF-hand domain containing 2 (Efhc2) as a novel component of genetic programme regulating nephron segmentation in zebrafish...
2018: Cell & Bioscience
Seon-Hwa Kim, Soo-Na Cho, Yun-Ji Lim, Ji-Ae Choi, Junghwan Lee, Dam Go, Chang-Hwa Song
Background: Mycobacterium smegmatis , a rapidly growing non-tuberculosis mycobacterium, is a good model for studying the pathogenesis of tuberculosis because of its genetic similarity to Mycobacterium tuberculosis (Mtb). Macrophages remove mycobacteria during an infection. Macrophage apoptosis is a host defense mechanism against intracellular bacteria. We have reported that endoplasmic reticulum (ER) stress is an important host defense mechanism against Mtb infection. Results: In this study, we found that M...
2018: Cell & Bioscience
Morihiro Okada, Yun-Bo Shi
Cell proliferation and differentiation are two distinct yet coupled processes in development in diverse organisms. Understanding the molecular mechanisms that regulate this process is a central theme in developmental biology. The intestinal epithelium is a highly complex tissue that relies on the coordination of cell proliferation within the crypts and apoptosis mainly at the tip of the villi, preservation of epithelial function through differentiation, and homeostatic cell migration along the crypt-villus axis...
2018: Cell & Bioscience
Jin Sung Koh, Moon Kyung Joo, Jong-Jae Park, Hyo Soon Yoo, Byung Il Choi, Beom Jae Lee, Hoon Jai Chun, Sang Woo Lee
Background: We investigated the inhibitory effect of pantoprazole on signal transducer and activator of transcription 3 (STAT3) activity and invasiveness of gastric adenocarcinoma cells, and the role of SH2-containing protein tyrosine phosphatase 1 (SHP-1) in mediating role. Methods: We used AGS and MKN-28 cells because of reduced SHP-1 and preserved p-STAT3 expression. Western blot, wound closure assay, Matrigel invasion assay and 3-D culture invasion assay were performed...
2018: Cell & Bioscience
Wenyu Jia, Zequn Li, Junyu Chen, Lei Sun, Chuanqian Liu, Shaping Wang, Jingwei Chi, Jun Niu, Hong Lai
Background: Tumor necrosis factor (TNF)-alpha-induced protein 8-like 2 (TIPE2 or TNFAIP8L2) is a newly described negative immune regulator and is closely associated with various tumors. However, the expression and roles of TIPE2 in PTC is unknown. Results: In the present study, TIPE2 upregulation in PTC tissues was found to be negatively associated with tumor size, capsule infiltration, peripheral infiltration and tumor T stage, which could be used to predict tumor invasiveness...
2018: Cell & Bioscience
Yilin Ye, Suk-Hwan Baek, Yihong Ye, Ting Zhang
Background: Endoplasmic reticulum (ER)-associated degradation (ERAD) regulates protein homeostasis in the secretory pathway by targeting misfolded or unassembled proteins for degradation by the proteasome. Hrd1 is a conserved multi-spanning membrane bound ubiquitin ligase required for ubiquitination of many aberrant ER proteins, but few endogenous substrates of Hrd1 have been identified to date. Methods: Using a SILAC-based quantitative proteomic approach combined with CRISPR-mediated gene silencing, we searched for endogenous physiological substrates of Hrd1...
2018: Cell & Bioscience
Wenjuan Bi, Yi Liu, Jing Guo, Zhen Lin, Jinsong Liu, Miao Zhou, Daniel Wismeijer, Janak L Pathak, Gang Wu
Background: Bone regenerative heterodimeric bone morphogenetic protein 2/7 (BMP2/7) enhances but all-trans retinoic acid (ATRA) inhibits osteoclastogenesis. However, the effect of ATRA on physiological and/or BMP2/7-induced osteoclastogenesis in still unclear. In this study, we aimed to test the effect of combined treatment of BMP2/7 and ATRA on osteoclastogenesis, and resorption activity. Results: All-trans retinoic acid (1 µM) ± BMP2/7 (5 or 50 ng/ml) was added in murine pre-osteoclasts cell line RAW264...
2018: Cell & Bioscience
Khampaseuth Thapa, Kai Connie Wu, Aishwarya Sarma, Eric M Grund, Angela Szeto, Armando J Mendez, Stephane Gesta, Vivek K Vishnudas, Niven R Narain, Rangaprasad Sarangarajan
Background: Diabetes mellitus is associated with an increased risk in diabetic cardiomyopathy (DCM) that is distinctly not attributed to co-morbidities with other vasculature diseases. To date, while dysregulation of calcium handling is a key hallmark in cardiomyopathy, studies have been inconsistent in the types of alterations involved. In this study human cardiomyocytes were exposed to an environmental nutritional perturbation of high glucose, fatty acids, and l-carnitine to model DCM and iTRAQ-coupled LC-MS/MS proteomic analysis was used to capture proteins affected by the perturbation...
2018: Cell & Bioscience
Yun-Bo Shi
Two research groups led by Dr. Shui Qing Ye of University of Missouri Kansas City School of Medicine, Kansas City, USA and Dr. Dihua Yu of University of Texas MD Anderson Cancer Center, Houston, USA, respectively, won the 2017 Ming K Jeang Award for Excellence in Cell & Bioscience.
2018: Cell & Bioscience
Liangru Fei, Hongtao Xu
A heterohexameric complex composed of minichromosome maintenance protein 2-7 (MCM2-7), which acts as a key replicative enzyme in eukaryotes, is crucial for initiating DNA synthesis only once per cell cycle. The MCM complex remains inactive through the G1 phase, until the S phase, when it is activated to initiate replication. During the transition from the G1 to S phase, the MCM undergoes multisite phosphorylation, an important change that promotes subsequent assembly of other replisome members. Phosphorylation is crucial for the regulation of MCM activity and function...
2018: Cell & Bioscience
Yakun Yin, Bin Zhao, Dongqin Li, Guangwen Yin
Background: Long non-coding RNAs (LncRNAs) have been identified as critical regulators in a variety of cancer types. Cancer susceptibility candidate 15 (CASC15), a lncRNA located at chromosome 6p22.3, has been discovered to participate in melanoma progression and phenotype switching. Nevertheless, the roles and molecular mechanisms of CASC15 in melanoma are far from being understood. Results: We found that CASC15 expression was up-regulated in melanoma tissues and associated with advanced pathological stages...
2018: Cell & Bioscience
Li Niu, Cuifang Ye, Yun Sun, Ting Peng, Shiming Yang, Weixi Wang, He Li
Background: Iron accumulation in basal ganglia accompanies neuronal loss in Huntington's disease (HD) patients and mouse disease models. Disruption of HD brain iron homeostasis occurs before the onset of clinical signs. Therefore, investigating the mechanism of iron accumulation is essential to understanding its role in disease pathogenesis. Methods: N171-82Q HD transgenic mice brain iron was detected by using Diaminobenzidine-enhanced Perls' stain. Iron homeostatic proteins including iron response protein 1 (IRP1), transferrin (Tf), ferritin and transferrin receptor (TfR) were determined by using western blotting and immunohistochemistry, and their relative expression levels of RNA were measured by RT-PCR in both N171-82Q HD transgenic mice and HEK293 cells expressing N-terminal of huntingtin...
2018: Cell & Bioscience
Qisheng Zuo, Chen Zhang, Kai Jin, Jin Jing, Changhua Sun, Mahmoud F Ahmed, Jiuzhou Song, Yani Zhang, Guohong Chen, Bichun Li
Background: Notch signaling is mainly regulated by Notch1 during development of chicken germ stem cells; however, the molecular mechanisms that contribute to generation of these germ stem cells have not been thoroughly investigated. Results: In our studies, Overexpression of the Notch1 NICD promoted development of the reproductive ridge, but inhibited the formation of seminiferous tubules. The formation efficiency of PGCs in the reproductive ridge following overexpression of NICD (7...
2018: Cell & Bioscience
Suaka Kagbo-Kue, Taiwo Ajose, Nicolas Bakinde
No abstract text is available yet for this article.
2018: Cell & Bioscience
Xuehong Xu, Steven P Balk, William B Isaacs, Jianjie Ma
No abstract text is available yet for this article.
2018: Cell & Bioscience
Zhoutao He, Xiaoning Sun, Zhichao Ma, Jiao Fu, Baili Huang, Fujin Liu, Yi Chen, Taozhi Deng, Xiangyang Han, Deming Sun, Cheng Lan
Background: This study investigated the role of HSP70 in modulating intestinal γδ T cells' Th17 response in Trichinella spiralis -induced PI-IBS mice model. Methods: The intestinal HSP70's expression and mRNA level were measured by Western blot and RT-PCR. The intestinal γδ T cell's morphological changes were analyzed using immunofluorescence staining and confocal laser scanning microscope. The pro-inflammatory cytokines' level was detected by ELISA. The isolated and purified γδ T cells were pre-incubated with HSP70 and their functions including proliferation, apoptosis, activation and production of IL-17 were also detected...
2018: Cell & Bioscience
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