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Cell & Bioscience

Jin Sung Koh, Moon Kyung Joo, Jong-Jae Park, Hyo Soon Yoo, Byung Il Choi, Beom Jae Lee, Hoon Jai Chun, Sang Woo Lee
Background: We investigated the inhibitory effect of pantoprazole on signal transducer and activator of transcription 3 (STAT3) activity and invasiveness of gastric adenocarcinoma cells, and the role of SH2-containing protein tyrosine phosphatase 1 (SHP-1) in mediating role. Methods: We used AGS and MKN-28 cells because of reduced SHP-1 and preserved p-STAT3 expression. Western blot, wound closure assay, Matrigel invasion assay and 3-D culture invasion assay were performed...
2018: Cell & Bioscience
Wenyu Jia, Zequn Li, Junyu Chen, Lei Sun, Chuanqian Liu, Shaping Wang, Jingwei Chi, Jun Niu, Hong Lai
Background: Tumor necrosis factor (TNF)-alpha-induced protein 8-like 2 (TIPE2 or TNFAIP8L2) is a newly described negative immune regulator and is closely associated with various tumors. However, the expression and roles of TIPE2 in PTC is unknown. Results: In the present study, TIPE2 upregulation in PTC tissues was found to be negatively associated with tumor size, capsule infiltration, peripheral infiltration and tumor T stage, which could be used to predict tumor invasiveness...
2018: Cell & Bioscience
Yilin Ye, Suk-Hwan Baek, Yihong Ye, Ting Zhang
Background: Endoplasmic reticulum (ER)-associated degradation (ERAD) regulates protein homeostasis in the secretory pathway by targeting misfolded or unassembled proteins for degradation by the proteasome. Hrd1 is a conserved multi-spanning membrane bound ubiquitin ligase required for ubiquitination of many aberrant ER proteins, but few endogenous substrates of Hrd1 have been identified to date. Methods: Using a SILAC-based quantitative proteomic approach combined with CRISPR-mediated gene silencing, we searched for endogenous physiological substrates of Hrd1...
2018: Cell & Bioscience
Wenjuan Bi, Yi Liu, Jing Guo, Zhen Lin, Jinsong Liu, Miao Zhou, Daniel Wismeijer, Janak L Pathak, Gang Wu
Background: Bone regenerative heterodimeric bone morphogenetic protein 2/7 (BMP2/7) enhances but all-trans retinoic acid (ATRA) inhibits osteoclastogenesis. However, the effect of ATRA on physiological and/or BMP2/7-induced osteoclastogenesis in still unclear. In this study, we aimed to test the effect of combined treatment of BMP2/7 and ATRA on osteoclastogenesis, and resorption activity. Results: All-trans retinoic acid (1 µM) ± BMP2/7 (5 or 50 ng/ml) was added in murine pre-osteoclasts cell line RAW264...
2018: Cell & Bioscience
Khampaseuth Thapa, Kai Connie Wu, Aishwarya Sarma, Eric M Grund, Angela Szeto, Armando J Mendez, Stephane Gesta, Vivek K Vishnudas, Niven R Narain, Rangaprasad Sarangarajan
Background: Diabetes mellitus is associated with an increased risk in diabetic cardiomyopathy (DCM) that is distinctly not attributed to co-morbidities with other vasculature diseases. To date, while dysregulation of calcium handling is a key hallmark in cardiomyopathy, studies have been inconsistent in the types of alterations involved. In this study human cardiomyocytes were exposed to an environmental nutritional perturbation of high glucose, fatty acids, and l-carnitine to model DCM and iTRAQ-coupled LC-MS/MS proteomic analysis was used to capture proteins affected by the perturbation...
2018: Cell & Bioscience
Yun-Bo Shi
Two research groups led by Dr. Shui Qing Ye of University of Missouri Kansas City School of Medicine, Kansas City, USA and Dr. Dihua Yu of University of Texas MD Anderson Cancer Center, Houston, USA, respectively, won the 2017 Ming K Jeang Award for Excellence in Cell & Bioscience.
2018: Cell & Bioscience
Liangru Fei, Hongtao Xu
A heterohexameric complex composed of minichromosome maintenance protein 2-7 (MCM2-7), which acts as a key replicative enzyme in eukaryotes, is crucial for initiating DNA synthesis only once per cell cycle. The MCM complex remains inactive through the G1 phase, until the S phase, when it is activated to initiate replication. During the transition from the G1 to S phase, the MCM undergoes multisite phosphorylation, an important change that promotes subsequent assembly of other replisome members. Phosphorylation is crucial for the regulation of MCM activity and function...
2018: Cell & Bioscience
Yakun Yin, Bin Zhao, Dongqin Li, Guangwen Yin
Background: Long non-coding RNAs (LncRNAs) have been identified as critical regulators in a variety of cancer types. Cancer susceptibility candidate 15 (CASC15), a lncRNA located at chromosome 6p22.3, has been discovered to participate in melanoma progression and phenotype switching. Nevertheless, the roles and molecular mechanisms of CASC15 in melanoma are far from being understood. Results: We found that CASC15 expression was up-regulated in melanoma tissues and associated with advanced pathological stages...
2018: Cell & Bioscience
Li Niu, Cuifang Ye, Yun Sun, Ting Peng, Shiming Yang, Weixi Wang, He Li
Background: Iron accumulation in basal ganglia accompanies neuronal loss in Huntington's disease (HD) patients and mouse disease models. Disruption of HD brain iron homeostasis occurs before the onset of clinical signs. Therefore, investigating the mechanism of iron accumulation is essential to understanding its role in disease pathogenesis. Methods: N171-82Q HD transgenic mice brain iron was detected by using Diaminobenzidine-enhanced Perls' stain. Iron homeostatic proteins including iron response protein 1 (IRP1), transferrin (Tf), ferritin and transferrin receptor (TfR) were determined by using western blotting and immunohistochemistry, and their relative expression levels of RNA were measured by RT-PCR in both N171-82Q HD transgenic mice and HEK293 cells expressing N-terminal of huntingtin...
2018: Cell & Bioscience
Qisheng Zuo, Chen Zhang, Kai Jin, Jin Jing, Changhua Sun, Mahmoud F Ahmed, Jiuzhou Song, Yani Zhang, Guohong Chen, Bichun Li
Background: Notch signaling is mainly regulated by Notch1 during development of chicken germ stem cells; however, the molecular mechanisms that contribute to generation of these germ stem cells have not been thoroughly investigated. Results: In our studies, Overexpression of the Notch1 NICD promoted development of the reproductive ridge, but inhibited the formation of seminiferous tubules. The formation efficiency of PGCs in the reproductive ridge following overexpression of NICD (7...
2018: Cell & Bioscience
Suaka Kagbo-Kue, Taiwo Ajose, Nicolas Bakinde
No abstract text is available yet for this article.
2018: Cell & Bioscience
Xuehong Xu, Steven P Balk, William B Isaacs, Jianjie Ma
No abstract text is available yet for this article.
2018: Cell & Bioscience
Zhoutao He, Xiaoning Sun, Zhichao Ma, Jiao Fu, Baili Huang, Fujin Liu, Yi Chen, Taozhi Deng, Xiangyang Han, Deming Sun, Cheng Lan
Background: This study investigated the role of HSP70 in modulating intestinal γδ T cells' Th17 response in Trichinella spiralis -induced PI-IBS mice model. Methods: The intestinal HSP70's expression and mRNA level were measured by Western blot and RT-PCR. The intestinal γδ T cell's morphological changes were analyzed using immunofluorescence staining and confocal laser scanning microscope. The pro-inflammatory cytokines' level was detected by ELISA. The isolated and purified γδ T cells were pre-incubated with HSP70 and their functions including proliferation, apoptosis, activation and production of IL-17 were also detected...
2018: Cell & Bioscience
Jian Zhang, Joseph R Lakowicz
Background: Near-field fluorescence (NFF) effects were employed to develop a novel near-infrared (NIR) luminescent nanoparticle (LNP) with superior brightness. The LNP is used as imaging contrast agent for cellular and small animal imaging and furthermore suggested to use for detecting voltage-sensitive calcium in living cells and animals with high sensitivity. Results: NIR Indocyanine green (ICG) dye was conjugated with human serum albumin (HSA) followed by covalently binding to gold nanorod (AuNR)...
2018: Cell & Bioscience
Zhao-Shan Liu, Zi-Yu Zhang, Hong Cai, Ming Zhao, Jie Mao, Jiang Dai, Tian Xia, Xue-Min Zhang, Tao Li
Background: As an important danger signal, the presence of DNA in cytoplasm triggers potent immune responses. Cyclic GMP-AMP synthase (cGAS) is a recently characterized key sensor for cytoplasmic DNA. The engagement of cGAS with DNA leads to the synthesis of a second messenger, cyclic GMP-AMP (cGAMP), which binds and activates the downstream adaptor protein STING to promote type I interferon production. Although cGAS has been shown to play a pivotal role in innate immunity, the exact regulation of cGAS activation is not fully understood...
2018: Cell & Bioscience
Fei Tang, Pan Zheng
Antibody blockade of the PD-1/PD-L1 pathway has elicited durable antitumor responses in the therapy of a broad spectrum of cancers. PD-L1 is constitutively expressed in certain tumors and host immune cells, and its expression can be induced or maintained by many factors. The expression of PD-L1 on tumor tissues has been reported to be positively correlated with the efficacy of anti-PD-1/PD-L1 therapy in patients. However, multiple clinical trials indicate that patients with PD-L1-negative tumors also respond to this blockade therapy, which suggests the potential contribution of PD-L1 from host immune cells...
2018: Cell & Bioscience
Chengcheng Li, Yi Luo, Lijian Shao, Aimin Meng, Daohong Zhou
Objective: Previous studies have shown that inhibition of inducible NO synthase (NOS2 or iNOS) with an inhibitor can selectively protect several normal tissues against radiation during radiotherapy. However, the role of NOS2 in ionizing radiation (IR)-induced bone marrow (BM) suppression is unknown and thus was investigated in the present study using NOS2- /- and wild-type mice 14 days after they were exposed to a sublethal dose of total body irradiation (TBI). Methods: The effects of different doses of IR (1, 2 and 4 Gy) on the apoptosis and colony-forming ability of bone marrow cells from wild-type (WT) and NOS2- /- mice were investigated in vitro...
2018: Cell & Bioscience
Souqi Liao, Wenyan Dong, Hui Zhao, Ruijin Huang, Xufeng Qi, Dongqing Cai
Two studies have recently focused on adult heart regeneration in Xenopus . While we reported on cardiac myogenic regeneration in Xenopus tropicalis after injury, Marshall and colleagues found no regeneration in an injured heart in Xenopus laevis. Here, we would like to join the discussion initiated by Marshall et al. who debated the methods and species differences in both studies. We agree with their view that the species difference in cardiac regenerative capacity could lead to different results in both of these studies...
2018: Cell & Bioscience
Lindsey Marshall, Fabrice Girardot, Barbara A Demeneix, Laurent Coen
We recently used an endoscopy-based resection method to explore the consequences of cardiac injury in adult Xenopus laevis , obtaining the result that the adult Xenopus heart is unable to regenerate. At 11 months post-amputation, cellular and biological marks of scarring persisted. We thus concluded that, contrary to urodeles and teleosts, adult anurans share a cardiac injury outcome similar to adult mammals. However, in their work published in this journal on the 13 December 2017, Liao et al. showed that the adult Xenopus tropicalis heart is capable of efficient, almost scar free regeneration, a result at odds with our previous observation...
2018: Cell & Bioscience
Fei Tang, Xuexiang Du, Mingyue Liu, Pan Zheng, Yang Liu
Antibodies to human CTLA-4 have been shown to induce long-lasting protection against melanoma. It is assumed that these antibodies cause tumor rejection by blocking negative signaling from the B7-CTLA-4 interactions to enhance priming of naïve T cells in the lymphoid organs. Recently, we reported that anti-CTLA-4 antibody Ipilimumab effectively induces tumor rejection in vivo although it blocks neither B7 transendocytosis by CTLA-4 nor CTLA-4 binding to immobilized or cell-associated B7. Using genetic model in which the anti-CTLA-4 antibodies are unable to engage more than 50% of CTLA-4, we demonstrated that saturating binding of CTLA-4 is not necessary for tumor rejection...
2018: Cell & Bioscience
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