journal
MENU ▼
Read by QxMD icon Read
search

Current Protocols in Mouse Biology

journal
https://www.readbyqxmd.com/read/29944194/a-method-for-parasagittal-sectioning-for-neuroanatomical-quantification-of-brain-structures-in-the-adult-mouse
#1
Stephan C Collins, Christel Wagner, Léo Gagliardi, Perrine F Kretz, Marie-Christine Fischer, Pascal Kessler, Meghna Kannan, Binnaz Yalcin
In this article, we present a standardized protocol for fast and robust neuroanatomical phenotyping of the adult mouse brain, which complements a previously published article (doi: 10.1002/cpmo.12) in Current Protocols in Mouse Biology. It is aimed at providing an experimental pipeline within an academic research setting from experimental work to data analysis. Our analysis focuses on one single parasagittal plane, covering the majority of brain regions involved in higher order cognitions such as the cortex, hippocampus, and cerebellum, for a total of 166 parameters of area, length, and cell-level measurements in contrast to 78 parameters in our previously published coronal screen...
June 26, 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29927555/assessment-of-topographic-memory-in-mice-in-a-complex-environment-using-the-hamlet-test
#2
Lucie Crouzier, Tangui Maurice
Here we provide instructions to measure topographic memory in mice using the Hamlet test, a complex environment. The apparatus mimics a small hamlet with a central agora and five houses, which are functionalized since mice can drink, eat pellets, hide within a small maze, run in an activity wheel, or interact with a stranger mouse behind a grid. The houses are interconnected through a network of streets in a five-arm star shape, and a video tracking system takes information from the activity in each house or follows a single mouse by trajectometry...
June 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29927554/mri-to-assess-neurological-function
#3
Brian J Nieman, Matthijs C van Eede, Shoshana Spring, Jun Dazai, R Mark Henkelman, Jason P Lerch
This article describes a detailed set of protocols for mouse brain imaging using MRI. We focus primarily on measuring changes in neuroanatomy, and provide both instructions for mouse preparation and details on image acquisition, image processing, and statistics. Practical details as well as theoretical considerations are provided. © 2018 by John Wiley & Sons, Inc.
June 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29927553/computational-analysis-of-neonatal-mouse-ultrasonic-vocalization
#4
Pilib Ó Broin, Michael V Beckert, Tomohisa Takahashi, Takeshi Izumi, Kenny Ye, Gina Kang, Patricia Pouso, Mackenzie Topolski, Jose L Pena, Noboru Hiroi
Neonatal vocalization is structurally altered in mouse models of autism spectrum disorder (ASD). Our published data showed that pup vocalization, under conditions of maternal separation, contains sequences whose alterations in a genetic mouse model of ASD impair social communication between pups and mothers. We describe details of a method which reveals the statistical structure of call sequences that are functionally critical for optimal maternal care. Entropy analysis determines the degree of non-random call sequencing...
June 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29927552/generation-and-identification-of-mutations-resulting-in-chronic-and-age-related-phenotypes-in-mice
#5
Andrew Blease, Thomas Nicol, Sara Falcone, Becky Starbuck, Simon Greenaway, Marie Hutchinson, Paul K Potter
Aging is inevitable, and our society must deal with the consequences: namely, an increased incidence of disease and ill health. Many mouse models of disease are acute or early onset or are induced in young mice, despite the fact that aging is a significant risk factor for a range of significant diseases. To improve modeling of such diseases, we should incorporate aging into our models. Many systems are affected by aging, with a decline in mitochondrial function, an increase in senescence, a loss of resilience, telomere shortening, and a decline in immune function being key factors in the increased susceptibility to disease that is associated with aging...
June 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29924918/assessing-healthspan-and-lifespan-measures-in-aging-mice-optimization-of-testing-protocols-replicability-and-rater-reliability
#6
Stacey J Sukoff Rizzo, Laura C Anderson, Torrian L Green, Tracy McGarr, Gaylynn Wells, Shawn S Winter
The relationship between chronological age (lifespan) and biological age (healthspan) varies amongst individuals. Understanding the normal trajectory and characteristic traits of aging mice throughout their lifespan is important for selecting the most reliable and reproducible measures to test hypotheses. The protocols herein describe assays used for aging studies at The Jackson Laboratory's Mouse Neurobehavioral Phenotyping Facility and include assessments of frailty, cognition, and sensory (hearing, vision, olfaction), motor, and fine motor function that can be used for assessing phenotypes in aged mice across their lifespan as well as provide guidance for setting up and validating these behavioral measures...
June 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29988984/production-of-humanized-mice-through-stem-cell-transfer
#7
Devra D Huey, Stefan Niewiesk
The development of humanized mice has become a prominent tool for translational animal studies of human diseases. Here we show how immune deficient mice can be "humanized" by injections of human umbilical cord stem cells. The engraftment of these cells and development into human lymphocytes has been possible because of the development of novel severely immune deficient mouse strains. Here we present proven protocols for the generation and analysis of humanized mice on the NSG mouse background.
March 2018: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29261233/evaluation-of-the-estrous-cycle-reproductive-tract-and-mammary-gland-in-female-mice
#8
Justin D Vidal, Adam J Filgo
Evaluation of the female reproductive system is an important part of basic reproductive biology research, toxicology testing, and mutant mouse phenotype assessment. The female reproductive system is dynamic and the onset of puberty and the normal changes observed during estrous cyclicity can create challenges for an investigator. Experimental work in the female mouse requires an understanding of the potential impact of the estrous cycle and tracking normal changes throughout the cycle allows for control of this key variable...
December 20, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29261232/a-mouse-model-of-west-nile-virus-infection
#9
Jessica B Graham, Jessica L Swarts, Jennifer M Lund
The use of a mouse model to study the breadth of symptoms and disease severity seen in human West Nile virus (WNV) infection can provide insight into the kinetics of the immune response and the specific pathways responsible for control of WNV infection and viral clearance. Here, we provide protocols for performing WNV infection of mice, as well as complete immunophenotyping analysis of the cellular immune response to infection in both the periphery and the central nervous system in a mouse model of WNV infection...
December 20, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29261231/the-study-of-host-immune-responses-elicited-by-the-model-murine-hookworms-nippostrongylus-brasiliensis-and-heligmosomoides-polygyrus
#10
T Bouchery, B Volpe, K Shah, L Lebon, K Filbey, G LeGros, N Harris
Hookworm infections (Necator americanus or Ancylostoma duodenale) represent a major neglected tropical disease, affecting approximately 700 million people worldwide, and can cause severe morbidity due to the need for these worms to feed on host blood. N. brasiliensis and H. polygrus, both rodent parasites, are the two most commonly employed laboratory models of experimental hookworm infection. Both parasites evoke type 2 immune responses, and their use has been instrumental in generating fundamental insight into the molecular mechanisms of type-2 immunity and for understanding how the immune response can control parasite numbers...
December 20, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29261230/assessing-sociability-social-memory-and-pup-retrieval-in-mice
#11
Annemarie Zimprich, Jörn Niessing, Lior Cohen, Lillian Garrett, Jan Einicke, Bettina Sperling, Mathias V Schmidt, Sabine M Hölter
Adaptive social behavior is important in mammals, both for the well-being of the individual and for the thriving of the species. Dysfunctions in social behavior occur in many neurodevelopmental and psychiatric diseases, and research into the genetic components of disease-relevant social deficits can open up new avenues for understanding the underlying biological mechanisms and therapeutic interventions. Genetically modified mouse models are particularly useful in this respect, and robust experimental protocols are needed to reliably assess relevant social behavior phenotypes...
December 20, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28884794/pathology-evaluation-of-developmental-phenotypes-in-neonatal-and-juvenile-mice
#12
Brad Bolon, Susan Newbigging, Kelli L Boyd
Necropsy (or autopsy) is the post mortem dissection of an animal to examine and collect organs and tissues in order to understand the effects and causes of disease. The systematic harvesting of samples at necropsy is an essential step in defining the reason for an unexpected death and in characterizing the features (i.e., phenotype) of a newly discovered condition. Phenotypic evaluation of young (neonatal and juvenile) mice emphasizes morphologic (macroscopic and microscopic) techniques and biochemical (clinical chemistry, hematologic, and molecular) analyses...
September 8, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28884793/using-vascular-landmarks-to-orient-3d-optical-coherence-tomography-images-of-the-mouse-eye
#13
Mark P Krebs
Comparing 3D structural information obtained by optical coherence tomography (OCT) requires accurate alignment of images acquired from individual subjects. Despite the widespread use of OCT to image the anterior and posterior mouse eye, few approaches to align the resulting image data have been described, in part due to a lack of well-characterized landmarks that are suitable for alignment. Here, we provide an OCT acquisition and analysis protocol that incorporates the use of the long posterior ciliary arteries as landmarks...
September 8, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28884792/profiling-of-single-cell-transcriptomes
#14
Wanze Chen, Vincent Gardeux, Antonio Meireles-Filho, Bart Deplancke
Complex biological systems are composed of multiple cell types whose transcriptional activity can vary due to differences in cell state, environmental stimulation, or intrinsic programs. Conventional bulk analysis methods capture the average transcriptional programs of the cell population, thus missing the unique cellular signature of each single cell. In recent years, the development of single-cell RNA-sequencing (scRNA-seq) technologies has provided a powerful approach to dissect the cellular heterogeneity of complex biological systems...
September 8, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28628219/application-of-swath-proteomics-to-mouse-biology
#15
Yibo Wu, Evan G Williams, Ruedi Aebersold
The quantitative measurement of the proteome has been shown to yield new insights into physiology and cell biology that cannot be determined from the genome and transcriptome because the quantitative relationship between transcriptome and proteome is complex. MS-based proteomics techniques, such as SWATH-MS, have recently advanced to the point at which they may be reliably applied by biologists who are not specialists in mass spectrometry. Here we provide standard protocols for preparation of tissue samples for input into the SWATH-MS analytical pipeline...
June 19, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28628218/microbiota-analysis-using-an-illumina-miseq-platform-to-sequence-16s-rrna-genes
#16
Alexis Rapin, Céline Pattaroni, Benjamin J Marsland, Nicola L Harris
The microbiota have been shown to play an important role in diverse biological processes including immunity, metabolism, and digestion. Assessing the exact composition of the microbiota has proven challenging due to the often unknown growth specificities of its members, and culture-based approaches typically fail to capture the complete diversity of microorganisms present. Next Generation Sequencing (NGS) methods provide an efficient means to gather information about cultured and uncultured members of the microbiota...
June 19, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28628217/mouse-model-of-burn-wound-and-infection-thermal-hot-air-lesion-induced-immunosuppression
#17
Henrik Calum, Niels Høiby, Claus Moser
The immunosuppression induced by thermal injury renders the burned victim susceptible to infection. A mouse model was developed to examine the immunosuppression, which was possible to induce even at a minor thermal insult of 6% total body surface area. After induction of the burn (48 hr) a depression of leukocytes in the peripheral blood was found of the burned mice. This depression was due to a reduction in the polymorphonuclear cells. The burned mice were not able to clear a Pseudomonas aeruginosa wound infection, since the infection spread to the blood as compared to mice only infected with P...
June 19, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28628216/a-fast-easy-and-customizable-eight-color-flow-cytometric-method-for-analysis-of-the-cellular-content-of-bronchoalveolar-lavage-fluid-in-the-mouse
#18
François Daubeuf, Julien Becker, Juan Antonio Aguilar-Pimentel, Claudine Ebel, Martin Hrabě de Angelis, Yann Hérault, Nelly Frossard
The cell composition of bronchoalveolar lavage fluid (BAL) is an important indicator of airway inflammation. It is commonly determined by cytocentrifuging leukocytes on slides, then staining, identifying, and counting them as eosinophils, neutrophils, macrophages, or lymphocytes according to morphological criteria under light microscopy, where it is not always easy to distinguish macrophages from lymphocytes. We describe here a one-step, easy-to-use, and easy-to-customize 8-color flow cytometric method for performing differential cell count and comparing it to morphological counts on stained cytospins...
June 19, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28628215/monitoring-pathogen-induced-sickness-in-mice-and-rats
#19
Daria Kolmogorova, Emma Murray, Nafissa Ismail
Sickness behavior monitoring, a technique for examining the development of sickness symptomatology following infection, is necessary in experiments studying neurochemical and physiological changes associated with pathogen-induced immune activation. However, the results of sickness behavior monitoring are difficult to reconcile due to inconsistencies in protocol methods and rater bias. The protocol described herein offers a non-invasive and unbiased approach to assess the progression of pathogen-induced sickness behaviors...
June 19, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/28252202/a-mouse-model-for-ocular-surface-staphylococcus-aureus-infection
#20
Zhiyong Zhang, Osama Abdel-Razek, Guirong Wang
Creation of an appropriate animal model that accurately reflects the disease and host immune response to bacterial infection in humans is a major challenge in ocular-surface infection research. For decades, mice have been the ideal small animal model for ocular-surface infection research because of the availability and relatively low cost of various genetic backgrounds, targeted defects, and immunologic reagents. By employing different combinations of mouse and bacterial strains, murine infection models can be used to explore a complete picture of bacterial infection and innate immunity of the ocular surface...
March 2, 2017: Current Protocols in Mouse Biology
journal
journal
43573
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"