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Journal of Laboratory Automation

Martin Adam, Heidi Fleischer, Kerstin Thurow
In the past year, automation has become more and more important in the field of elemental and structural chemical analysis to reduce the high degree of manual operation and processing time as well as human errors. Thus, a high number of data points are generated, which requires fast and automated data evaluation. To handle the preprocessed export data from different analytical devices with software from various vendors offering a standardized solution without any programming knowledge should be preferred. In modern laboratories, multiple users will use this software on multiple personal computers with different operating systems (e...
October 13, 2016: Journal of Laboratory Automation
Kristine M Mayle, Kathryn R Dern, Vincent K Wong, Kevin Y Chen, Shijun Sung, Ke Ding, April R Rodriguez, Scott Knowles, Zachary Taylor, Z Hong Zhou, Warren S Grundfest, Anna M Wu, Timothy J Deming, Daniel T Kamei
Currently, there is no curative treatment for advanced metastatic prostate cancer, and options, such as chemotherapy, are often nonspecific, harming healthy cells and resulting in severe side effects. Attaching targeting ligands to agents used in anticancer therapies has been shown to improve efficacy and reduce nonspecific toxicity. Furthermore, the use of triggered therapies can enable spatial and temporal control over the treatment. Here, we combined an engineered prostate cancer-specific targeting ligand, the A11 minibody, with a novel photothermal therapy agent, polypeptide-based gold nanoshells, which generate heat in response to near-infrared light...
September 22, 2016: Journal of Laboratory Automation
Christopher M Weikart, Alexander M Klibanov, Adam P Breeland, Ahmad H Taha, Brian R Maurer, Steven P Martin
SiO2 Medical Products, Inc. (SiO) has developed a proprietary technology that greatly enhances protein recoveries and reduces extractables from commercial microplates used for bioanalytical assays and storage of biologics. SiO technology is based on plasma treatment that chemically modifies the surface of polypropylene with predominantly hydrogen-bond-acceptor uncharged polar groups. The resultant surface resists nonspecific protein adsorption over a wide range of protein concentrations, thereby eliminating the need to passivate (and hence potentially contaminate) the microplates with blocking proteins...
September 20, 2016: Journal of Laboratory Automation
Hans-Christian Luedemann
Low-coherence interferometry is presented as a method for nondestructive, noncontact, and high-resolution measurement of liquid volumes and fill heights in microplates. A Meniscense (Bolton, MA) prototype system captures both fill height and meniscus shape and combines them into a volume measurement, allowing for the measurement of a wide range of liquids with different meniscus shapes. The system has a liquid fill height resolution of 0.7 µm, corresponding to a volume resolution of 0.02 µL in a typical 96-well plate, demonstrated in an ideal model system...
September 19, 2016: Journal of Laboratory Automation
Abhishek Pathak, Jayant Borana, Jayesh Vasudeva Adhikari, Sai Siva Gorthi
In this article, we report on the development of indicator-impregnated agarose sensor films for colorimetric estimation of the pH of different standard buffer solutions and urine samples. In contrast to the conventional paper-based dipstick method, the presented method employs dried agarose that was infused with a chemical indicator, which when exposed to the test sample results in an appropriate chromogenic outcome. The color change was then quantified by the smart camera system. The divulged instrument has been calibrated with standard buffers and also tested with clinical urine samples...
September 19, 2016: Journal of Laboratory Automation
Israel T Desta, Abdelrazak Al-Sharif, Nour AlGharibeh, Nahal Mustafa, Ajymurat Orozaliev, Nikolaos Giakoumidis, Kristin C Gunsalus, Yong-Ak Song
Microfluidic devices offer new technical possibilities for a precise manipulation of Caenorhabditis elegans due to the comparable length scale. C. elegans is a small, free-living nematode worm that is a popular model system for genetic, genomic, and high-throughput experimental studies of animal development and neurobiology. In this paper, we demonstrate a microfluidic system in polydimethylsiloxane (PDMS) for dispensing of a single C. elegans worm into a 96-well plate. It consists of two PDMS layers, a flow and a control layer...
September 14, 2016: Journal of Laboratory Automation
Akhil Chaturvedi, Sai Siva Gorthi
Portable microfluidic diagnostic devices, including flow cytometers, are being developed for point-of-care settings, especially in conjunction with inexpensive imaging devices such as mobile phone cameras. However, two pervasive drawbacks of these have been the lack of automated sample preparation processes and cells settling out of sample suspensions, leading to inaccurate results. We report an automated blood sample preparation unit (ABSPU) to prevent blood samples from settling in a reservoir during loading of samples in flow cytometers...
August 24, 2016: Journal of Laboratory Automation
Tal Murthy
No abstract text is available yet for this article.
October 2016: Journal of Laboratory Automation
Heidi Fleischer, Robert Ralf Drews, Jessica Janson, Bharath Reddy Chinna Patlolla, Xianghua Chu, Michael Klos, Kerstin Thurow
Automation systems with applied robotics have already been established in industrial applications for many years. In the field of life sciences, a comparable high level of automation can be found in the areas of bioscreening and high-throughput screening. Strong deficits still exist in the development of flexible and universal fully automated systems in the field of analytical measurement. Reasons are the heterogeneous processes with complex structures, which include sample preparation and transport, analytical measurements using complex sensor systems, and suitable data analysis and evaluation...
October 2016: Journal of Laboratory Automation
David I Walsh, Shashi K Murthy, Aman Russom
Point-of-care (POC) microfluidic devices often lack the integration of common sample preparation steps, such as preconcentration, which can limit their utility in the field. In this technology brief, we describe a system that combines the necessary sample preparation methods to perform sample-to-result analysis of large-volume (20 mL) biopsy model samples with staining of captured cells. Our platform combines centrifugal-paper microfluidic filtration and an analysis system to process large, dilute biological samples...
October 2016: Journal of Laboratory Automation
Laurent Bessemans, Vanessa Jully, Caroline de Raikem, Mathieu Albanese, Nicolas Moniotte, Pascal Silversmet, Dominique Lemoine
High-throughput screening technologies are increasingly integrated into the formulation development process of biopharmaceuticals. The performance of liquid handling systems is dependent on the ability to deliver accurate and precise volumes of specific reagents to ensure process quality. We have developed an automated gravimetric calibration procedure to adjust the accuracy and evaluate the precision of the TECAN Freedom EVO liquid handling system. Volumes from 3 to 900 µL using calibrated syringes and fixed tips were evaluated with various solutions, including aluminum hydroxide and phosphate adjuvants, β-casein, sucrose, sodium chloride, and phosphate-buffered saline...
October 2016: Journal of Laboratory Automation
Haoyi Jin, Yanqiu Yu
High-quality preclinical bioassay models are essential for drug research and development. We reviewed the emerging body-on-a-chip technology, which serves as a promising model to overcome the limitations of traditional bioassay models, and introduced existing models of body-on-a-chip, their constitutional details, application for drug testing, and individual features of these models. We put special emphasis on the latest trend in this field of incorporating barrier tissue into body-on-a-chip and discussed several remaining challenges of current body-on-a-chip...
October 2016: Journal of Laboratory Automation
Jiancheng Li, Bizhen Cheng, Li Yang, Ying Zhao, Meichen Pan, Gaozhe Zheng, Xiaoyan Xu, Jing Hu, Tongtong Xiao, Yingmu Cai
Autoverification is a process of using computer-based rules to verify clinical laboratory test results without manual review. But to date, there are few published articles on the use of autoverification over the course of years in a clinical laboratory. In our study, we firstly described the development and implementation of autoverification rules for enzyme-linked immunosorbent assay (ELISA) results of hepatitis B virus (HBV) serological markers in a clinical immunology laboratory. We designed the autoverification rules for HBV by using Boolean logic on five clinically used serological markers in accordance with the framework of AUTO-10A, issued by the American Clinical Laboratory Standards Institute in 2006...
October 2016: Journal of Laboratory Automation
Ellen Vorberg, Heidi Fleischer, Steffen Junginger, Hui Liu, Norbert Stoll, Kerstin Thurow
Life science areas require specific sample pretreatment to increase the concentration of the analytes and/or to convert the analytes into an appropriate form for the detection and separation systems. Various workstations are commercially available, allowing for automated biological sample pretreatment. Nevertheless, due to the required temperature, pressure, and volume conditions in typical element and structure-specific measurements, automated platforms are not suitable for analytical processes. Thus, the purpose of the presented investigation was the design, realization, and evaluation of an automated system ensuring high-precision sample preparation for a variety of analytical measurements...
October 2016: Journal of Laboratory Automation
Abdul Rashid Husain, Yaser Hadad, Muhd Nazrul Hisham Zainal Alam
This article presents the development of a low-cost microcontroller-based interface for a microbioreactor operation. An Arduino MEGA 2560 board with 54 digital input/outputs, including 15 pulse-width-modulation outputs, has been chosen to perform the acquisition and control of the microbioreactor. The microbioreactor (volume = 800 µL) was made of poly(dimethylsiloxane) and poly(methylmethacrylate) polymers. The reactor was built to be equipped with sensors and actuators for the control of reactor temperature and the mixing speed...
October 2016: Journal of Laboratory Automation
Matjaž Finšgar, Jennifer Jackson
In this work, the electrochemical potentiodynamic behavior of AISI C1018 lower-grade steel material was investigated in 20 wt.% methanesulfonic acid (MSA) solutions with or without different components to design corrosion inhibitor formulations based on acetylenic alcohol, cinnamaldehyde, 1-dodecylpyridinium chloride, and methanol. MSA has recently been considered as a new potential acid to be used in the matrix stimulation procedure and in well cleaning. It is demonstrated that AISI C1018 steel MSA needs to be inhibited...
October 2016: Journal of Laboratory Automation
Yung-Shin Sun, Xiangdong Zhu
Microarrays provide a platform for high-throughput characterization of biomolecular interactions. To increase the sensitivity and specificity of microarrays, surface blocking is required to minimize the nonspecific interactions between analytes and unprinted yet functionalized surfaces. To block amine- or epoxy-functionalized substrates, bovine serum albumin (BSA) is one of the most commonly used blocking reagents because it is cheap and easy to use. Based on standard protocols from microarray manufactories, a BSA concentration of 1% (10 mg/mL or 200 μM) and reaction time of at least 30 min are required to efficiently block epoxy-coated slides...
October 2016: Journal of Laboratory Automation
Hai-Yan Bie, Jian-Jun Ye, Zong-Rui Hao
Submerged exhaust noise is one of the main noise sources of underwater vehicles. The nozzle features of pipe discharging systems have a great influence on exhaust noise, especially on the noise produced by gas-liquid two-phase flow outside the nozzle. To study the influence of nozzle geometry on underwater jet noises, a theoretical study was performed on the critical weber number at which the jet flow field morphology changes. The underwater jet noise experiments of different nozzles under various working conditions were carried out...
October 2016: Journal of Laboratory Automation
Xiaole Mao, Tal Murthy
No abstract text is available yet for this article.
August 2016: Journal of Laboratory Automation
James M Gill, Alden S Hart
No abstract text is available yet for this article.
August 2016: Journal of Laboratory Automation
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