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Journal of Laboratory Automation

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https://www.readbyqxmd.com/read/28095179/development-of-fully-automated-low-cost-immunoassay-system-for-research-applications
#1
Guochun Wang, Champak Das, Bradley Ledden, Qian Sun, Chien Nguyen
Enzyme-linked immunosorbent assay (ELISA) automation for routine operation in a small research environment would be very attractive. A portable fully automated low-cost immunoassay system was designed, developed, and evaluated with several protein analytes. It features disposable capillary columns as the reaction sites and uses real-time calibration for improved accuracy. It reduces the overall assay time to less than 75 min with the ability of easy adaptation of new testing targets. The running cost is extremely low due to the nature of automation, as well as reduced material requirements...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28095178/current-trends-in-multidrug-optimization
#2
Andrea Weiss, Patrycja Nowak-Sliwinska
The identification of effective and long-lasting cancer therapies still remains elusive, partially due to patient and tumor heterogeneity, acquired drug resistance, and single-drug dose-limiting toxicities. The use of drug combinations may help to overcome some limitations of current cancer therapies by challenging the robustness and redundancy of biological processes. However, effective drug combination optimization requires the careful consideration of numerous parameters. The complexity of this optimization problem is clearly nontrivial and likely requires the assistance of advanced heuristic optimization techniques...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28027447/dynamic-compound-dependent-acoustic-transfer-to-investigate-inhibitor-reversibility
#3
Jennifer Nothstein, Elisabeth MacColl, Paul Zuck, Jason Cassaday, Victor N Uebele, Jeffrey D Hermes, Michelle F Homsher
Automated mechanism of action studies are introducing the need for tailored compound delivery, which can be challenging for standard compound management procedures. Jump dilution assays investigating inhibitor reversibility require compound delivery at specific volumes to assay specific concentrations of 10 × IC50 for each inhibitor. Creating custom-made source plates with unique compound concentrations to dispense a uniform single volume can be prohibitively slow. A broadly applicable tool that enables on-the fly dispensing of variable amounts of stock concentrations was developed using the Acoustic Transfer System (ATS)...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28027446/current-trends-in-multidrug-optimization
#4
Andrea Weiss, Patrycja Nowak-Sliwinska
The identification of effective and long-lasting cancer therapies still remains elusive, partially due to patient and tumor heterogeneity, acquired drug resistance, and single-drug dose-limiting toxicities. The use of drug combinations may help to overcome some limitations of current cancer therapies by challenging the robustness and redundancy of biological processes. However, effective drug combination optimization requires the careful consideration of numerous parameters. The complexity of this optimization problem is clearly nontrivial and likely requires the assistance of advanced heuristic optimization techniques...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27920397/optimizing-combination-therapy-for-acute-lymphoblastic-leukemia-using-a-phenotypic-personalized-medicine-digital-health-platform
#5
Dong-Keun Lee, Vivian Y Chang, Theodore Kee, Chih-Ming Ho, Dean Ho
Acute lymphoblastic leukemia (ALL) is a blood cancer that is characterized by the overproduction of lymphoblasts in the bone marrow. Treatment for pediatric ALL typically uses combination chemotherapy in phases, including a prolonged maintenance phase with oral methotrexate and 6-mercaptopurine, which often requires dose adjustment to balance side effects and efficacy. However, a major challenge confronting combination therapy for virtually every disease indication is the inability to pinpoint drug doses that are optimized for each patient, and the ability to adaptively and continuously optimize these doses to address comorbidities and other patient-specific physiological changes...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27908978/potential-of-laboratory-execution-systems-less-to-simplify-the-application-of-business-process-management-systems-bpmss-in-laboratory-automation
#6
Sebastian Neubert, Bernd Göde, Xiangyu Gu, Norbert Stoll, Kerstin Thurow
Modern business process management (BPM) is increasingly interesting for laboratory automation. End-to-end workflow automation and improved top-level systems integration for information technology (IT) and automation systems are especially prominent objectives. With the ISO Standard Business Process Model and Notation (BPMN) 2.X, a system-independent and interdisciplinary accepted graphical process control notation is provided, allowing process analysis, while also being executable. The transfer of BPM solutions to structured laboratory automation places novel demands, for example, concerning the real-time-critical process and systems integration...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28095177/integrated-colony-imaging-analysis-and-selection-device-for-regenerative-medicine
#7
Edward Kwee, Edward E Herderick, Thomas Adams, James Dunn, Robert Germanowski, Frank Krakosh, Cynthia Boehm, James Monnich, Kimerly Powell, George Muschler
Stem and progenitor cells derived from human tissues are being developed as cell sources for cell-based assays and therapies. However, tissue-derived stem and progenitor cells are heterogeneous. Differences in observed clones of stem cells likely reflect important aspects of the underlying state of the source cells, as well as future potency for cell therapies. This paper describes a colony analysis and picking device that provides quantitative analysis of heterogeneous cell populations and precise tools for cell picking for research or biomanufacturing applications...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28095176/a-flexible-workflow-for-automated-bioluminescent-kinase-selectivity-profiling
#8
Tracy Worzella, Matt Butzler, Jacquelyn Hennek, Seth Hanson, Laura Simdon, Said Goueli, Cris Cowan, Hicham Zegzouti
Kinase profiling during drug discovery is a necessary process to confirm inhibitor selectivity and assess off-target activities. However, cost and logistical limitations prevent profiling activities from being performed in-house. We describe the development of an automated and flexible kinase profiling workflow that combines ready-to-use kinase enzymes and substrates in convenient eight-tube strips, a bench-top liquid handling device, ADP-Glo Kinase Assay (Promega, Madison, WI) technology to quantify enzyme activity, and a multimode detection instrument...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28095175/evaluation-of-the-droplet-microarray-platform-for-high-throughput-screening-of-suspension-cells
#9
Anna A Popova, Claire Depew, Katya Manuella Permana, Alexander Trubitsyn, Ravindra Peravali, Jorge Ángel González Ordiano, Markus Reischl, Pavel A Levkin
Phenotypic cell-based high-throughput screenings play a central role in drug discovery and toxicology. The main tendency in cell screenings is the increase of the throughput and decrease of reaction volume in order to accelerate the experiments, reduce the costs, and enable screenings of rare cells. Conventionally, cell-based assays are performed in microtiter plates, which exist in 96- to 1536-wells formats and cannot be further miniaturized. In addition, performing screenings of suspension cells is associated with risk of losing cell content during the staining procedures and incompatibility with high-content microscopy...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27899700/a-magneto-microfluidic-system-for-investigating-the-influence-of-an-externally-induced-force-gradient-in-a-collagen-type-i-ecm-on-hmvec-sprouting
#10
Sahan C B Herath, Soheila Sharghi-Namini, Yue Du, Dongan Wang, Ruowen Ge, Qing-Guo Wang, Harry Asada, Peter C Y Chen
Advances in mechanobiology have suggested that physiological and pathological angiogenesis may be differentiated based on the ways in which the cells interact with the extracellular matrix (ECM) that exhibits partially different mechanical properties. This warrants investigating the regulation of ECM stiffness on cell behavior using angiogenesis assays. In this article, we report the application of the technique of active manipulation of ECM stiffness to study in vitro angiogenic sprouting of human microvascular endothelial cells (HMVECs) in a microfluidic device...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27899699/a-smartphone-based-genotyping-method-for-hepatitis-b-virus-at-point-of-care-settings
#11
Huiqin Jiang, Di Wu, Liuwei Song, Quan Yuan, Shengxiang Ge, Xiaoping Min, Ningshao Xia, Shizhi Qian, Xianbo Qiu
We reported a rapid, convenient, and easy-to-use genotyping method for hepatitis B virus (HBV) based on the smartphone at point-of-care (POC) settings. To perform HBV genotyping especially for genotypes A, B, C, and D, a smartphone is used to image and analyze a one-step immunoassay lateral flow strip functionalized with genotype-specific monoclonal antibodies (mAbs) on multiple capture lines. A light-emitting diode (LED) positioned on the top of the lateral flow strip is used to shine the multiple capture lines for excitation...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27879409/new-technologies-for-rapid-bacterial-identification-and-antibiotic-resistance-profiling
#12
Shana O Kelley
Conventional approaches to bacterial identification and drug susceptibility testing typically rely on culture-based approaches that take 2 to 7 days to return results. The long turnaround times contribute to the spread of infectious disease, negative patient outcomes, and the misuse of antibiotics that can contribute to antibiotic resistance. To provide new solutions enabling faster bacterial analysis, a variety of approaches are under development that leverage single-cell analysis, microfluidic concentration and detection strategies, and ultrasensitive readout mechanisms...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27879408/a-dual-label-time-resolved-fluorescence-immunoassay-for-the-simultaneous-determination-of-cardiac-troponin-t-and-myoglobin
#13
Ling Wang, Mujuan Xu, Ruolan Huang, Xiao Chang, Cuicui Chen, Laiqing Li, Zhong Zhang, Yue Han
The aim of this study was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of cardiac troponin T (cTnT) and myoglobin (MYO) for the early diagnosis of acute myocardial infarction. The sandwich immunoassay was used to detect the concentration of cTnT and MYO in serum. cTnT and MYO in serum were captured by anti-cTnT and anti-MYO antibodies immobilized on microtiter wells and then banded together with another anti-cTnT and anti-MYO labeled with europium(III) Sm(3+) and samarium(III) Eu(3+) chelates, followed by fluorescence measurement using time-resolved fluorometry...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27864340/microfluidic-tissue-mesodissection-in-molecular-cancer-diagnostics
#14
Christine Surrette, David Shoudy, Alex Corwin, Wei Gao, Maria I Zavodszky, Stanislav L Karsten, Todd Miller, Michael J Gerdes, Nichole Wood, John R Nelson, Chris M Puleo
We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-selected region from standard glass slide mounts. In this manner, costs associated with dissection remain low, but spatial resolution is compromised. In contrast, laser microdissection methods maintain spatial resolution that matches the requirements for analysis of important tissue heterogeneity but remains costly and labor intensive...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27864339/development-of-a-platform-to-enable-fully-automated-cross-titration-experiments
#15
Jason Cassaday, Michael Finley, Brian Squadroni, Sylvie Jezequel-Sur, Albert Rauch, Bharti Gajera, Victor Uebele, Jeffrey Hermes, Paul Zuck
In the triage of hits from a high-throughput screening campaign or during the optimization of a lead compound, it is relatively routine to test compounds at multiple concentrations to determine potency and maximal effect. Additional follow-up experiments, such as agonist shift, can be quite valuable in ascertaining compound mechanism of action (MOA). However, these experiments require cross-titration of a test compound with the activating ligand of the receptor requiring 100-200 data points, severely limiting the number tested in MOA assays in a screening triage...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27856945/microfluidic-cartridges-for-automated-point-of-care-blood-cell-counting
#16
Suzanne Smith, Phophi Madzivhandila, René Sewart, Ureshnie Govender, Holger Becker, Pieter Roux, Kevin Land
Disposable, low-cost microfluidic cartridges for automated blood cell counting applications are presented in this article. The need for point-of-care medical diagnostic tools is evident, particularly in low-resource and rural settings, and a full blood count is often the first step in patient diagnosis. Total white and red blood cell counts have been implemented toward a full blood count, using microfluidic cartridges with automated sample introduction and processing steps for visual microscopy cell counting to be performed...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27811326/a-low-cost-accurate-and-high-precision-fluid-dispensing-system-for-microscale-application
#17
Champak Das, Guochun Wang, Chien Nguyen
We present here the development of a low-cost, accurate, and precise fluid dispensing system. It can be used with peristaltic or any other pump to improve the flow characteristics. The dispensing system has a range of 1 to 100 µL with accuracy of ~99.5% and standard deviation at ~150 nL over the entire range. The system developed does not depend on the accuracy or precision of the driving pump; therefore, any positive displacement pump can be used to get similar accuracy and precision, which gives an opportunity to reduce the cost of the system...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27867154/life-sciences-discovery-and-technology-highlights
#18
David Yeo, Tal Murthy
No abstract text is available yet for this article.
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27325106/curcumin-in-treating-breast-cancer-a-review
#19
REVIEW
Yiwei Wang, Jiayi Yu, Ran Cui, Jinjin Lin, Xianting Ding
Breast cancer is among the most common malignant tumors. It is the second leading cause of cancer mortality among women in the United States. Curcumin, an active derivative from turmeric, has been reported to have anticancer and chemoprevention effects on breast cancer. Curcumin exerts its anticancer effect through a complicated molecular signaling network, involving proliferation, estrogen receptor (ER), and human epidermal growth factor receptor 2 (HER2) pathways. Experimental evidence has shown that curcumin also regulates apoptosis and cell phase-related genes and microRNA in breast cancer cells...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26956578/a-wide-linearity-range-method-for-the-determination-of-lenalidomide-in-plasma-by-high-performance-liquid-chromatography-application-to-pharmacokinetic-studies
#20
Beatriz Guglieri-López, Alejandro Pérez-Pitarch, Maria Amparo Martinez-Gómez, Begoña Porta-Oltra, Mónica Climente-Martí, Matilde Merino-Sanjuán
A wide linearity range analytical method for the determination of lenalidomide in patients with multiple myeloma for pharmacokinetic studies is required. Plasma samples were ultrasonicated for protein precipitation. A solid-phase extraction was performed. The eluted samples were evaporated to dryness under vacuum, and the solid obtained was diluted and injected into the high-performance liquid chromatography (HPLC) system. Separation of lenalidomide was performed on an Xterra RP C18 (250 mm length × 4.6 mm i...
December 2016: Journal of Laboratory Automation
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