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Journal of Laboratory Automation

Sebastian Neubert, Bernd Göde, Xiangyu Gu, Norbert Stoll, Kerstin Thurow
Modern business process management (BPM) is increasingly interesting for laboratory automation. End-to-end workflow automation and improved top-level systems integration for information technology (IT) and automation systems are especially prominent objectives. With the ISO Standard Business Process Model and Notation (BPMN) 2.X, a system-independent and interdisciplinary accepted graphical process control notation is provided, allowing process analysis, while also being executable. The transfer of BPM solutions to structured laboratory automation places novel demands, for example, concerning the real-time-critical process and systems integration...
December 1, 2016: Journal of Laboratory Automation
Sahan C B Herath, Soheila Sharghi-Namini, Yue Du, Dongan Wang, Ruowen Ge, Qing-Guo Wang, Harry Asada, Peter C Y Chen
Advances in mechanobiology have suggested that physiological and pathological angiogenesis may be differentiated based on the ways in which the cells interact with the extracellular matrix (ECM) that exhibits partially different mechanical properties. This warrants investigating the regulation of ECM stiffness on cell behavior using angiogenesis assays. In this article, we report the application of the technique of active manipulation of ECM stiffness to study in vitro angiogenic sprouting of human microvascular endothelial cells (HMVECs) in a microfluidic device...
November 29, 2016: Journal of Laboratory Automation
Huiqin Jiang, Di Wu, Liuwei Song, Quan Yuan, Shengxiang Ge, Xiaoping Min, Ningshao Xia, Shizhi Qian, Xianbo Qiu
We reported a rapid, convenient, and easy-to-use genotyping method for hepatitis B virus (HBV) based on the smartphone at point-of-care (POC) settings. To perform HBV genotyping especially for genotypes A, B, C, and D, a smartphone is used to image and analyze a one-step immunoassay lateral flow strip functionalized with genotype-specific monoclonal antibodies (mAbs) on multiple capture lines. A light-emitting diode (LED) positioned on the top of the lateral flow strip is used to shine the multiple capture lines for excitation...
November 29, 2016: Journal of Laboratory Automation
Shana O Kelley
Conventional approaches to bacterial identification and drug susceptibility testing typically rely on culture-based approaches that take 2 to 7 days to return results. The long turnaround times contribute to the spread of infectious disease, negative patient outcomes, and the misuse of antibiotics that can contribute to antibiotic resistance. To provide new solutions enabling faster bacterial analysis, a variety of approaches are under development that leverage single-cell analysis, microfluidic concentration and detection strategies, and ultrasensitive readout mechanisms...
November 22, 2016: Journal of Laboratory Automation
Ling Wang, Mujuan Xu, Ruolan Huang, Xiao Chang, Cuicui Chen, Laiqing Li, Zhong Zhang, Yue Han
The aim of this study was to establish a dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of cardiac troponin T (cTnT) and myoglobin (MYO) for the early diagnosis of acute myocardial infarction. The sandwich immunoassay was used to detect the concentration of cTnT and MYO in serum. cTnT and MYO in serum were captured by anti-cTnT and anti-MYO antibodies immobilized on microtiter wells and then banded together with another anti-cTnT and anti-MYO labeled with europium(III) Sm(3+) and samarium(III) Eu(3+) chelates, followed by fluorescence measurement using time-resolved fluorometry...
November 22, 2016: Journal of Laboratory Automation
Christine Surrette, David Shoudy, Alex Corwin, Wei Gao, Maria I Zavodszky, Stanislav L Karsten, Todd Miller, Michael J Gerdes, Nichole Wood, John R Nelson, Chris M Puleo
We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-selected region from standard glass slide mounts. In this manner, costs associated with dissection remain low, but spatial resolution is compromised. In contrast, laser microdissection methods maintain spatial resolution that matches the requirements for analysis of important tissue heterogeneity but remains costly and labor intensive...
November 18, 2016: Journal of Laboratory Automation
Jason Cassaday, Michael Finley, Brian Squadroni, Sylvie Jezequel-Sur, Albert Rauch, Bharti Gajera, Victor Uebele, Jeffrey Hermes, Paul Zuck
In the triage of hits from a high-throughput screening campaign or during the optimization of a lead compound, it is relatively routine to test compounds at multiple concentrations to determine potency and maximal effect. Additional follow-up experiments, such as agonist shift, can be quite valuable in ascertaining compound mechanism of action (MOA). However, these experiments require cross-titration of a test compound with the activating ligand of the receptor requiring 100-200 data points, severely limiting the number tested in MOA assays in a screening triage...
November 18, 2016: Journal of Laboratory Automation
Suzanne Smith, Phophi Madzivhandila, René Sewart, Ureshnie Govender, Holger Becker, Pieter Roux, Kevin Land
Disposable, low-cost microfluidic cartridges for automated blood cell counting applications are presented in this article. The need for point-of-care medical diagnostic tools is evident, particularly in low-resource and rural settings, and a full blood count is often the first step in patient diagnosis. Total white and red blood cell counts have been implemented toward a full blood count, using microfluidic cartridges with automated sample introduction and processing steps for visual microscopy cell counting to be performed...
November 17, 2016: Journal of Laboratory Automation
Champak Das, Guochun Wang, Chien Nguyen
We present here the development of a low-cost, accurate, and precise fluid dispensing system. It can be used with peristaltic or any other pump to improve the flow characteristics. The dispensing system has a range of 1 to 100 µL with accuracy of ~99.5% and standard deviation at ~150 nL over the entire range. The system developed does not depend on the accuracy or precision of the driving pump; therefore, any positive displacement pump can be used to get similar accuracy and precision, which gives an opportunity to reduce the cost of the system...
November 3, 2016: Journal of Laboratory Automation
Wendao You, Liang Xu, Xing Zhang, Huan Zou, Dongtao Shi, Hanshuo Zhang, Juan Li, Weichang Chen, Rui Li
MicroRNAs (miRNAs) are globally dysregulated in human carcinomas. However, the specific miRNAs that mediate gastric cancer metastasis have not been identified. We identified 100 miRNAs that are dysregulated in gastric cancer and used a self-assembled cell microarray method to systematically evaluate their capacity to regulate cell migration. MiR-451, which is down-regulated in human gastric cancer samples, potently modulated multiple metastatic phenotypes including cell migration, invasion, proliferation, and epithelial-mesenchymal transition...
October 25, 2016: Journal of Laboratory Automation
Martin Adam, Heidi Fleischer, Kerstin Thurow
In the past year, automation has become more and more important in the field of elemental and structural chemical analysis to reduce the high degree of manual operation and processing time as well as human errors. Thus, a high number of data points are generated, which requires fast and automated data evaluation. To handle the preprocessed export data from different analytical devices with software from various vendors offering a standardized solution without any programming knowledge should be preferred. In modern laboratories, multiple users will use this software on multiple personal computers with different operating systems (e...
October 13, 2016: Journal of Laboratory Automation
Kristine M Mayle, Kathryn R Dern, Vincent K Wong, Kevin Y Chen, Shijun Sung, Ke Ding, April R Rodriguez, Scott Knowles, Zachary Taylor, Z Hong Zhou, Warren S Grundfest, Anna M Wu, Timothy J Deming, Daniel T Kamei
Currently, there is no curative treatment for advanced metastatic prostate cancer, and options, such as chemotherapy, are often nonspecific, harming healthy cells and resulting in severe side effects. Attaching targeting ligands to agents used in anticancer therapies has been shown to improve efficacy and reduce nonspecific toxicity. Furthermore, the use of triggered therapies can enable spatial and temporal control over the treatment. Here, we combined an engineered prostate cancer-specific targeting ligand, the A11 minibody, with a novel photothermal therapy agent, polypeptide-based gold nanoshells, which generate heat in response to near-infrared light...
September 22, 2016: Journal of Laboratory Automation
Christopher M Weikart, Alexander M Klibanov, Adam P Breeland, Ahmad H Taha, Brian R Maurer, Steven P Martin
SiO2 Medical Products, Inc. (SiO) has developed a proprietary technology that greatly enhances protein recoveries and reduces extractables from commercial microplates used for bioanalytical assays and storage of biologics. SiO technology is based on plasma treatment that chemically modifies the surface of polypropylene with predominantly hydrogen-bond-acceptor uncharged polar groups. The resultant surface resists nonspecific protein adsorption over a wide range of protein concentrations, thereby eliminating the need to passivate (and hence potentially contaminate) the microplates with blocking proteins...
September 20, 2016: Journal of Laboratory Automation
Hans-Christian Luedemann
Low-coherence interferometry is presented as a method for nondestructive, noncontact, and high-resolution measurement of liquid volumes and fill heights in microplates. A Meniscense (Bolton, MA) prototype system captures both fill height and meniscus shape and combines them into a volume measurement, allowing for the measurement of a wide range of liquids with different meniscus shapes. The system has a liquid fill height resolution of 0.7 µm, corresponding to a volume resolution of 0.02 µL in a typical 96-well plate, demonstrated in an ideal model system...
September 19, 2016: Journal of Laboratory Automation
Abhishek Pathak, Jayant Borana, Jayesh Vasudeva Adhikari, Sai Siva Gorthi
In this article, we report on the development of indicator-impregnated agarose sensor films for colorimetric estimation of the pH of different standard buffer solutions and urine samples. In contrast to the conventional paper-based dipstick method, the presented method employs dried agarose that was infused with a chemical indicator, which when exposed to the test sample results in an appropriate chromogenic outcome. The color change was then quantified by the smart camera system. The divulged instrument has been calibrated with standard buffers and also tested with clinical urine samples...
September 19, 2016: Journal of Laboratory Automation
Israel T Desta, Abdelrazak Al-Sharif, Nour AlGharibeh, Nahal Mustafa, Ajymurat Orozaliev, Nikolaos Giakoumidis, Kristin C Gunsalus, Yong-Ak Song
Microfluidic devices offer new technical possibilities for a precise manipulation of Caenorhabditis elegans due to the comparable length scale. C. elegans is a small, free-living nematode worm that is a popular model system for genetic, genomic, and high-throughput experimental studies of animal development and neurobiology. In this paper, we demonstrate a microfluidic system in polydimethylsiloxane (PDMS) for dispensing of a single C. elegans worm into a 96-well plate. It consists of two PDMS layers, a flow and a control layer...
September 14, 2016: Journal of Laboratory Automation
David Yeo, Tal Murthy
No abstract text is available yet for this article.
December 2016: Journal of Laboratory Automation
Yiwei Wang, Jiayi Yu, Ran Cui, Jinjin Lin, Xianting Ding
Breast cancer is among the most common malignant tumors. It is the second leading cause of cancer mortality among women in the United States. Curcumin, an active derivative from turmeric, has been reported to have anticancer and chemoprevention effects on breast cancer. Curcumin exerts its anticancer effect through a complicated molecular signaling network, involving proliferation, estrogen receptor (ER), and human epidermal growth factor receptor 2 (HER2) pathways. Experimental evidence has shown that curcumin also regulates apoptosis and cell phase-related genes and microRNA in breast cancer cells...
December 2016: Journal of Laboratory Automation
Beatriz Guglieri-López, Alejandro Pérez-Pitarch, Maria Amparo Martinez-Gómez, Begoña Porta-Oltra, Mónica Climente-Martí, Matilde Merino-Sanjuán
A wide linearity range analytical method for the determination of lenalidomide in patients with multiple myeloma for pharmacokinetic studies is required. Plasma samples were ultrasonicated for protein precipitation. A solid-phase extraction was performed. The eluted samples were evaporated to dryness under vacuum, and the solid obtained was diluted and injected into the high-performance liquid chromatography (HPLC) system. Separation of lenalidomide was performed on an Xterra RP C18 (250 mm length × 4.6 mm i...
December 2016: Journal of Laboratory Automation
Darren McMorran, Dwayne Chung Kim Chung, Jonathan Li, Murat Muradoglu, Oi Wah Liew, Tuck Wah Ng
Flexible automation systems provide the needed adaptability to serve shorter-term projects and specialty applications in biochemical analysis. A low-cost selective compliant articulated robotic arm designed for liquid spillage avoidance is developed here. In the vertical-plane robotic arm movement test, the signals from an inertial measurement unit (IMU) and accelerometer were able to sense collisions. In the horizontal movement test, however, only the signals from the IMU enabled collision to be detected. Using a calculation method developed, it was possible to chart the regions where the obstacle was likely to be located when a collision occurred...
December 2016: Journal of Laboratory Automation
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