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Journal of Laboratory Automation

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https://www.readbyqxmd.com/read/28095179/development-of-fully-automated-low-cost-immunoassay-system-for-research-applications
#1
Guochun Wang, Champak Das, Bradley Ledden, Qian Sun, Chien Nguyen
Enzyme-linked immunosorbent assay (ELISA) automation for routine operation in a small research environment would be very attractive. A portable fully automated low-cost immunoassay system was designed, developed, and evaluated with several protein analytes. It features disposable capillary columns as the reaction sites and uses real-time calibration for improved accuracy. It reduces the overall assay time to less than 75 min with the ability of easy adaptation of new testing targets. The running cost is extremely low due to the nature of automation, as well as reduced material requirements...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28095178/current-trends-in-multidrug-optimization
#2
Andrea Weiss, Patrycja Nowak-Sliwinska
The identification of effective and long-lasting cancer therapies still remains elusive, partially due to patient and tumor heterogeneity, acquired drug resistance, and single-drug dose-limiting toxicities. The use of drug combinations may help to overcome some limitations of current cancer therapies by challenging the robustness and redundancy of biological processes. However, effective drug combination optimization requires the careful consideration of numerous parameters. The complexity of this optimization problem is clearly nontrivial and likely requires the assistance of advanced heuristic optimization techniques...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28027447/dynamic-compound-dependent-acoustic-transfer-to-investigate-inhibitor-reversibility
#3
Jennifer Nothstein, Elisabeth MacColl, Paul Zuck, Jason Cassaday, Victor N Uebele, Jeffrey D Hermes, Michelle F Homsher
Automated mechanism of action studies are introducing the need for tailored compound delivery, which can be challenging for standard compound management procedures. Jump dilution assays investigating inhibitor reversibility require compound delivery at specific volumes to assay specific concentrations of 10 × IC50 for each inhibitor. Creating custom-made source plates with unique compound concentrations to dispense a uniform single volume can be prohibitively slow. A broadly applicable tool that enables on-the fly dispensing of variable amounts of stock concentrations was developed using the Acoustic Transfer System (ATS)...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28027446/current-trends-in-multidrug-optimization
#4
Andrea Weiss, Patrycja Nowak-Sliwinska
The identification of effective and long-lasting cancer therapies still remains elusive, partially due to patient and tumor heterogeneity, acquired drug resistance, and single-drug dose-limiting toxicities. The use of drug combinations may help to overcome some limitations of current cancer therapies by challenging the robustness and redundancy of biological processes. However, effective drug combination optimization requires the careful consideration of numerous parameters. The complexity of this optimization problem is clearly nontrivial and likely requires the assistance of advanced heuristic optimization techniques...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27920397/optimizing-combination-therapy-for-acute-lymphoblastic-leukemia-using-a-phenotypic-personalized-medicine-digital-health-platform
#5
Dong-Keun Lee, Vivian Y Chang, Theodore Kee, Chih-Ming Ho, Dean Ho
Acute lymphoblastic leukemia (ALL) is a blood cancer that is characterized by the overproduction of lymphoblasts in the bone marrow. Treatment for pediatric ALL typically uses combination chemotherapy in phases, including a prolonged maintenance phase with oral methotrexate and 6-mercaptopurine, which often requires dose adjustment to balance side effects and efficacy. However, a major challenge confronting combination therapy for virtually every disease indication is the inability to pinpoint drug doses that are optimized for each patient, and the ability to adaptively and continuously optimize these doses to address comorbidities and other patient-specific physiological changes...
December 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27867154/life-sciences-discovery-and-technology-highlights
#6
David Yeo, Tal Murthy
No abstract text is available yet for this article.
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27325106/curcumin-in-treating-breast-cancer-a-review
#7
REVIEW
Yiwei Wang, Jiayi Yu, Ran Cui, Jinjin Lin, Xianting Ding
Breast cancer is among the most common malignant tumors. It is the second leading cause of cancer mortality among women in the United States. Curcumin, an active derivative from turmeric, has been reported to have anticancer and chemoprevention effects on breast cancer. Curcumin exerts its anticancer effect through a complicated molecular signaling network, involving proliferation, estrogen receptor (ER), and human epidermal growth factor receptor 2 (HER2) pathways. Experimental evidence has shown that curcumin also regulates apoptosis and cell phase-related genes and microRNA in breast cancer cells...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26956578/a-wide-linearity-range-method-for-the-determination-of-lenalidomide-in-plasma-by-high-performance-liquid-chromatography-application-to-pharmacokinetic-studies
#8
Beatriz Guglieri-López, Alejandro Pérez-Pitarch, Maria Amparo Martinez-Gómez, Begoña Porta-Oltra, Mónica Climente-Martí, Matilde Merino-Sanjuán
A wide linearity range analytical method for the determination of lenalidomide in patients with multiple myeloma for pharmacokinetic studies is required. Plasma samples were ultrasonicated for protein precipitation. A solid-phase extraction was performed. The eluted samples were evaporated to dryness under vacuum, and the solid obtained was diluted and injected into the high-performance liquid chromatography (HPLC) system. Separation of lenalidomide was performed on an Xterra RP C18 (250 mm length × 4.6 mm i...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26882923/adapting-a-low-cost-selective-compliant-articulated-robotic-arm-for-spillage-avoidance
#9
Darren McMorran, Dwayne Chung Kim Chung, Jonathan Li, Murat Muradoglu, Oi Wah Liew, Tuck Wah Ng
Flexible automation systems provide the needed adaptability to serve shorter-term projects and specialty applications in biochemical analysis. A low-cost selective compliant articulated robotic arm designed for liquid spillage avoidance is developed here. In the vertical-plane robotic arm movement test, the signals from an inertial measurement unit (IMU) and accelerometer were able to sense collisions. In the horizontal movement test, however, only the signals from the IMU enabled collision to be detected. Using a calculation method developed, it was possible to chart the regions where the obstacle was likely to be located when a collision occurred...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26856613/implementation-of-an-automated-high-throughput-plasmid-dna-production-pipeline
#10
Karen Billeci, Christopher Suh, Tina Di Ioia, Lovejit Singh, Ryan Abraham, Anne Baldwin, Stephen Monteclaro
Biologics sample management facilities are often responsible for a diversity of large-molecule reagent types, such as DNA, RNAi, and protein libraries. Historically, the management of large molecules was dispersed into multiple laboratories. As methodologies to support pathway discovery, antibody discovery, and protein production have become high throughput, the implementation of automation and centralized inventory management tools has become important. To this end, to improve sample tracking, throughput, and accuracy, we have implemented a module-based automation system integrated into inventory management software using multiple platforms (Hamilton, Hudson, Dynamic Devices, and Brooks)...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26846143/applying-dataflow-architecture-and-visualization-tools-to-in-vitro-pharmacology-data-automation
#11
David Pechter, Serena Xu, Marc Kurtz, Steven Williams, Lisa Sonatore, Artjohn Villafania, Sony Agrawal
The pace and complexity of modern drug discovery places ever-increasing demands on scientists for data analysis and interpretation. Data flow programming and modern visualization tools address these demands directly. Three different requirements-one for allosteric modulator analysis, one for a specialized clotting analysis, and one for enzyme global progress curve analysis-are reviewed, and their execution in a combined data flow/visualization environment is outlined.
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26745976/a-simple-and-easy-process-for-the-determination-of-estimated-plasma-glucose-level-in-patients-presenting-to-hospital-an-example-of-multicentric-data-mining
#12
Muhittin A Serdar, Macit Koldaş, Mustafa Serteser, Okhan Akın, Cigdem Sonmez, Ozlem Gülbahar, Filiz Akbıyık, Ibrahim Ünsal
The aim of the present study was to determine the relation between the simultaneous fasting plasma glucose level and HbA1c in a large population of patients presenting to the hospital, based on various measurement methods available for HbA1c. HbA1c levels of 162,210 patients presenting to various hospitals and laboratories were measured based on seven different systems, and at the same time, eAG levels were calculated based on HbA1c levels. The correlation coefficients (r) between serum plasma glucose and HbA1c levels were found to be 0...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26702022/consolidating-a-distributed-compound-management-capability-into-a-single-installation-the-application-of-overall-equipment-effectiveness-to-determine-capacity-utilization
#13
Clive Green, Daniel Taylor
Compound management (CM) is a critical discipline enabling hit discovery through the production of assay-ready compound plates for screening. CM in pharma requires significant investments in manpower, capital equipment, repairs and maintenance, and information technology. These investments are at risk from external factors, for example, new technology rendering existing equipment obsolete and strategic site closures. At AstraZeneca, we faced the challenge of evaluating the number of CM sites required to support hit discovery in response to site closures and pressure on our operating budget...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26702021/automated-patch-clamp-meets-high-throughput-screening-384-cells-recorded-in-parallel-on-a-planar-patch-clamp-module
#14
Alison Obergrussberger, Andrea Brüggemann, Tom A Goetze, Markus Rapedius, Claudia Haarmann, Ilka Rinke, Nadine Becker, Takayuki Oka, Atsushi Ohtsuki, Timo Stengel, Marius Vogel, Juergen Steindl, Max Mueller, Johannes Stiehler, Michael George, Niels Fertig
We have developed an automated patch clamp module for high-throughput ion channel screening, recording from 384 cells simultaneously. The module is incorporated into a laboratory pipetting robot and uses a 384-channel pipettor head for application of cells and compounds. The module contains 384 amplifier channels for fully parallel recordings using a digital amplifier. Success rates for completed experiments (1- to 4-point concentration-response curves for cells satisfying defined quality control parameters) of greater than 85% have been routinely achieved with, for example, HEK, CHO, and RBL cell lines expressing hNaV1...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26702020/automated-processing-of-plasma-samples-for-lipoprotein-separation-by-rate-zonal-ultracentrifugation
#15
Carl N Peters, Iain E J Evans
Plasma lipoproteins are the primary means of lipid transport among tissues. Defining alterations in lipid metabolism is critical to our understanding of disease processes. However, lipoprotein measurement is limited to specialized centers. Preparation for ultracentrifugation involves the formation of complex density gradients that is both laborious and subject to handling errors. We created a fully automated device capable of forming the required gradient. The design has been made freely available for download by the authors...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26668185/toward-automated-interpretation-of-lc-ms-data-for-quality-assurance-of-a-screening-collection
#16
Daniel H Addison
The AstraZeneca Compound Management group uses high-performance liquid chromatography-mass spectrometry for structure elucidation and purity determination of the AstraZeneca compound collection. These activities are conducted in a high-throughput environment where the rate-limiting step is the review and interpretation of analytical results, which is time-consuming and experience dependent. Despite the development of a semiautomated review system, manual interpretation of results remains a bottleneck. Data-mining techniques were applied to archived data to further automate the review process...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26194105/automated-device-for-asynchronous-extraction-of-rna-dna-or-protein-biomarkers-from-surrogate-patient-samples
#17
Anna L Bitting, Hali Bordelon, Mark L Baglia, Keersten M Davis, Amy E Creecy, Philip A Short, Laura E Albert, Aditya V Karhade, David W Wright, Frederick R Haselton, Nicholas M Adams
Many biomarker-based diagnostic methods are inhibited by nontarget molecules in patient samples, necessitating biomarker extraction before detection. We have developed a simple device that purifies RNA, DNA, or protein biomarkers from complex biological samples without robotics or fluid pumping. The device design is based on functionalized magnetic beads, which capture biomarkers and remove background biomolecules by magnetically transferring the beads through processing solutions arrayed within small-diameter tubing...
December 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27899700/a-magneto-microfluidic-system-for-investigating-the-influence-of-an-externally-induced-force-gradient-in-a-collagen-type-i-ecm-on-hmvec-sprouting
#18
Sahan C B Herath, Soheila Sharghi-Namini, Yue Du, Dongan Wang, Ruowen Ge, Qing-Guo Wang, Harry Asada, Peter C Y Chen
Advances in mechanobiology have suggested that physiological and pathological angiogenesis may be differentiated based on the ways in which the cells interact with the extracellular matrix (ECM) that exhibits partially different mechanical properties. This warrants investigating the regulation of ECM stiffness on cell behavior using angiogenesis assays. In this article, we report the application of the technique of active manipulation of ECM stiffness to study in vitro angiogenic sprouting of human microvascular endothelial cells (HMVECs) in a microfluidic device...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27864340/microfluidic-tissue-mesodissection-in-molecular-cancer-diagnostics
#19
Christine Surrette, David Shoudy, Alex Corwin, Wei Gao, Maria I Zavodszky, Stanislav L Karsten, Todd Miller, Michael J Gerdes, Nichole Wood, John R Nelson, Chris M Puleo
We present a mesodissection platform that retains the advantages of laser-based dissection instrumentation with the speed and ease of manual dissection. Tissue dissection in clinical laboratories is often performed by manually scraping a physician-selected region from standard glass slide mounts. In this manner, costs associated with dissection remain low, but spatial resolution is compromised. In contrast, laser microdissection methods maintain spatial resolution that matches the requirements for analysis of important tissue heterogeneity but remains costly and labor intensive...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/27491328/life-sciences-discovery-and-technology-highlights
#20
Tal Murthy
No abstract text is available yet for this article.
October 2016: Journal of Laboratory Automation
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