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Cellular Logistics

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https://www.readbyqxmd.com/read/27738552/neo1-and-phosphatidylethanolamine-contribute-to-vacuole-membrane-fusion-in-saccharomyces-cerevisiae
#1
Yuantai Wu, Mehmet Takar, Andrea A Cuentas-Condori, Todd R Graham
NEO1 is an essential gene in budding yeast and belongs to a highly conserved subfamily of P-type ATPase genes that encode phospholipid flippases. Inactivation of temperature sensitive neo1(ts) alleles produces pleiomorphic defects in the secretory and endocytic pathways, including fragmented vacuoles. A screen for multicopy suppressors of neo1-2(ts) growth defects yielded YPT7, which encodes a Rab7 homolog involved in SNARE-dependent vacuolar fusion. YPT7 suppressed the vacuole fragmentation phenotype of neo1-2, but did not suppress Golgi-associated protein trafficking defects...
July 2016: Cellular Logistics
https://www.readbyqxmd.com/read/27738551/an-improved-reversibly-dimerizing-mutant-of-the-fk506-binding-protein-fkbp
#2
Juan J Barrero, Effrosyni Papanikou, Jason C Casler, Kasey J Day, Benjamin S Glick
FK506-binding protein (FKBP) is a monomer that binds to FK506, rapamycin, and related ligands. The F36M substitution, in which Phe36 in the ligand-binding pocket is changed to Met, leads to formation of antiparallel FKBP dimers, which can be dissociated into monomers by ligand binding. This FKBP(M) mutant has been employed in the mammalian secretory pathway to generate aggregates that can be dissolved by ligand addition to create cargo waves. However, when testing this approach in yeast, we found that dissolution of FKBP(M) aggregates was inefficient...
July 2016: Cellular Logistics
https://www.readbyqxmd.com/read/27294009/allosteric-properties-of-ph-domains-in-arf-regulatory-proteins
#3
Neeladri Sekhar Roy, Marielle E Yohe, Paul A Randazzo, James M Gruschus
Pleckstrin Homology (PH) domains bind phospholipids and proteins. They are critical regulatory elements of a number enzymes including guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) for Ras-superfamily guanine nucleotide binding proteins such as ADP-ribosylation factors (Arfs). Recent studies have indicated that many PH domains may bind more than one ligand cooperatively. Here we discuss the molecular basis of PH domain-dependent allosteric behavior of 2 ADP-ribosylation factor exchange factors, Grp1 and Brag2, cooperative binding of ligands to the PH domains of Grp1 and the Arf GTPase-activating protein, ASAP1, and the consequences for activity of the associated catalytic domains...
April 2016: Cellular Logistics
https://www.readbyqxmd.com/read/27217979/small-gtpases-in-trafficking-a-family-approach-introducing-a-rolling-series-focused-on-groups-or-families-of-small-gtpases-in-trafficking
#4
Jennifer L Stow
No abstract text is available yet for this article.
January 2016: Cellular Logistics
https://www.readbyqxmd.com/read/27217978/degradation-elements-coincide-with-cofactor-binding-sites-in-a-short-lived-transcription-factor
#5
Christopher M Hickey
Elaborate control of gene expression by transcription factors is common to all kingdoms of life. In eukaryotes, transcription factor abundance and activity are often regulated by targeted proteolysis via the ubiquitin-proteasome system (UPS). The yeast MATα2 (α2) cell type regulator has long served as a model for UPS-dependent transcription factor degradation. Proteolysis of α2 is complex: it involves at least 2 ubiquitylation pathways and multiple regions of α2 affect its degradation. Such complexity also exists for the degradation of other UPS substrates...
January 2016: Cellular Logistics
https://www.readbyqxmd.com/read/27217977/sequential-recruitment-of-rab-gtpases-during-early-stages-of-phagocytosis
#6
Jeremy C Yeo, Adam A Wall, Lin Luo, Jennifer L Stow
The phagocytosis and destruction of pathogens and dead cells by macrophages is important for innate immunity and tissue maintenance. Multiple Rab family GTPases engage effector molecules to coordinate the early stages of phagocytosis, which include rapid changes in actin polymerization, membrane phospholipids, trafficking and the activation of receptors. Defining the spatiotemporal, sequential recruitment of these Rabs is critical for insights into how phagocytosis is initiated and coordinated. Here, we screened GFP-tagged Rabs expressed in fixed and live cells to identify and stratify those recruited to early phagocytic membranes at stages defined by phospholipid transitions...
January 2016: Cellular Logistics
https://www.readbyqxmd.com/read/27054048/plasma-membrane-regulates-ras-signaling-networks
#7
Tanmay Sanjeev Chavan, Serena Muratcioglu, Richard Marszalek, Hyunbum Jang, Ozlem Keskin, Attila Gursoy, Ruth Nussinov, Vadim Gaponenko
Ras GTPases activate more than 20 signaling pathways, regulating such essential cellular functions as proliferation, survival, and migration. How Ras proteins control their signaling diversity is still a mystery. Several pieces of evidence suggest that the plasma membrane plays a critical role. Among these are: (1) selective recruitment of Ras and its effectors to particular localities allowing access to Ras regulators and effectors; (2) specific membrane-induced conformational changes promoting Ras functional diversity; and (3) oligomerization of membrane-anchored Ras to recruit and activate Raf...
October 2015: Cellular Logistics
https://www.readbyqxmd.com/read/27054047/rock1-and-rock2-inhibition-alters-dendritic-spine-morphology-in-hippocampal-neurons
#8
Sharon A Swanger, Alexa L Mattheyses, Erik G Gentry, Jeremy H Herskowitz
Communication among neurons is mediated through synaptic connections between axons and dendrites, and most excitatory synapses occur on actin-rich protrusions along dendrites called dendritic spines. Dendritic spines are structurally dynamic, and synapse strength is closely correlated with spine morphology. Abnormalities in the size, shape, and number of dendritic spines are prevalent in neurologic diseases, including autism spectrum disorders, schizophrenia, and Alzheimer disease. However, therapeutic targets that influence spine morphology are lacking...
October 2015: Cellular Logistics
https://www.readbyqxmd.com/read/27057421/tool-box-plasmids-for-the-expression-or-knockdown-of-human-arf-family-gtpases-arf-arl-sar-and-their-co-expression-in-bacteria-with-n-myristoyltransferases
#9
Shana C Kerr, Richard A Kahn
No abstract text is available yet for this article.
July 2015: Cellular Logistics
https://www.readbyqxmd.com/read/27057420/arf-like-gtpase-arl8-moving-from-the-periphery-to-the-center-of-lysosomal-biology
#10
Divya Khatter, Aastha Sindhwani, Mahak Sharma
Lysosomes are dynamic organelles that not only mediate degradation of cellular substrates but also play critical roles in processes such as cholesterol homeostasis, plasma membrane repair, antigen presentation, and cell migration. The small GTPase Arl8, a member of Arf-like (Arl) family of proteins, has recently emerged as a crucial regulator of lysosome positioning and membrane trafficking toward lysosomes. Through interaction with its effector SKIP, the human Arl8 paralog (Arl8b) mediates kinesin-1 dependent motility of lysosomes on microtubule tracks toward the cell periphery...
July 2015: Cellular Logistics
https://www.readbyqxmd.com/read/27057419/diacylglycerol-kinases-in-membrane-trafficking
#11
Shuwei Xie, Naava Naslavsky, Steve Caplan
Diacylglycerol kinases (DGKs) belong to a family of cytosolic kinases that regulate the phosphorylation of diacylglycerol (DAG), converting it into phosphatidic acid (PA). There are 10 known mammalian DGK isoforms, each with a different tissue distribution and substrate specificity. These differences allow regulation of cellular responses by fine-tuning the delicate balance of cellular DAG and PA. DGK isoforms are best characterized as mediators of signal transduction and immune function. However, since recent studies reveal that DAG and PA are also involved in the regulation of endocytic trafficking, it is therefore anticipated that DGKs also plays an important role in membrane trafficking...
April 2015: Cellular Logistics
https://www.readbyqxmd.com/read/27057418/role-of-the-epithelial-cell-specific-clathrin-adaptor-complex-ap-1b-in-cell-polarity
#12
Heike Fölsch
Epithelial cells are important for organ development and function. To this end, they polarize their plasma membrane into biochemically and physically distinct membrane domains. The apical membrane faces the luminal site of an organ and the basolateral domain is in contact with the basement membrane and neighboring cells. To establish and maintain this polarity it is important that newly synthesized and endocytic cargos are correctly sorted according to their final destinations at either membrane. Sorting takes place at one of 2 major sorting stations in the cells, the trans-Golgi network (TGN) and recycling endosomes (REs)...
April 2015: Cellular Logistics
https://www.readbyqxmd.com/read/26196023/role-of-tetanus-neurotoxin-insensitive-vesicle-associated-membrane-protein-in-membrane-domains-transport-and-homeostasis
#13
Diana Molino, Sébastien Nola, Sin Man Lam, Agathe Verraes, Véronique Proux-Gillardeaux, Gaëlle Boncompain, Franck Perez, Markus Wenk, Guanghou Shui, Lydia Danglot, Thierry Galli
Biological membranes in eukaryotes contain a large variety of proteins and lipids often distributed in domains in plasma membrane and endomembranes. Molecular mechanisms responsible for the transport and the organization of these membrane domains along the secretory pathway still remain elusive. Here we show that vesicular SNARE TI-VAMP/VAMP7 plays a major role in membrane domains composition and transport. We found that the transport of exogenous and endogenous GPI-anchored proteins was altered in fibroblasts isolated from VAMP7-knockout mice...
January 2015: Cellular Logistics
https://www.readbyqxmd.com/read/26196022/an-optimized-talen-application-for-mutagenesis-and-screening-in-drosophila-melanogaster
#14
Han B Lee, Zachary L Sebo, Ying Peng, Yi Guo
Transcription activator-like effector nucleases (TALENs) emerged as powerful tools for locus-specific genome engineering. Due to the ease of TALEN assembly, the key to streamlining TALEN-induced mutagenesis lies in identifying efficient TALEN pairs and optimizing TALEN mRNA injection concentrations to minimize the effort to screen for mutant offspring. Here we present a simple methodology to quantitatively assess bi-allelic TALEN cutting, as well as approaches that permit accurate measures of somatic and germline mutation rates in Drosophila melanogaster...
January 2015: Cellular Logistics
https://www.readbyqxmd.com/read/25767741/interaction-with-the-effector-dynamin-related-protein-1-drp1-is-an-ancient-function-of-rab32-subfamily-proteins
#15
Carolina G Ortiz-Sandoval, Sarah C Hughes, Joel B Dacks, Thomas Simmen
The mitochondria-associated membrane (MAM) is an endoplasmic reticulum (ER) domain that forms contacts with mitochondria and accommodates Ca(2+) transfer between the two organelles. The GTPase Rab32 regulates this function of the MAM via determining the localization of the Ca(2+) regulatory transmembrane protein calnexin to the MAM. Another function of the MAM is the regulation of mitochondrial dynamics mediated by GTPases such as dynamin-related protein 1 (Drp1). Consistent with the importance of the MAM for mitochondrial dynamics and the role of Rab32 in MAM enrichment, the inactivation of Rab32 leads to mitochondrial collapse around the nucleus...
October 2014: Cellular Logistics
https://www.readbyqxmd.com/read/25750764/the-vps39-like-trap1-is-an-effector-of-rab5-and-likely-the-missing-vps3-subunit-of-human-corvet
#16
Jens Lachmann, Elina Glaubke, Patrick S Moore, Christian Ungermann
Membrane fusion in the endocytic pathway is mediated by a protein machinery consistent of Rab GTPases, tethering factors and SNAREs. In yeast, the endosomal CORVET and lysosomal HOPS tethering complexes share 4 of their 6 subunits. The 2 additional subunits in each complex - Vps3 and Vps8 for CORVET, and the homologous Vps39 and Vps41 for HOPS - bind directly to Rab5 and Rab7, respectively. In humans, all subunits for HOPS have been described. However, human CORVET remains poorly characterized and a homolog of Vps3 is still missing...
October 2014: Cellular Logistics
https://www.readbyqxmd.com/read/25610722/ypt-rab-gtpases-regulate-two-intersections-of-the-secretory-and-the-endosomal-lysosomal-pathways
#17
Zhanna Lipatova, Nava Segev
A prevailing question in the Ypt/Rab field is whether these conserved GTPases are specific to cellular compartments. The established role for Ypt1 and its human homolog Rab1 is in endoplasmic reticulum (ER)-to-Golgi transport. More recently these regulators were implicated also in autophagy. Two different TRAPP complexes, I and III, were identified as the guanine-nucleotide-exchange factors (GEFs) of Ypt1 in ER-to-Golgi transport and autophagy, respectively. Confusingly, Ypt1 and TRAPP III were also suggested to regulate endosome-to-Golgi transport, implying that they function at multiple cellular compartments, and bringing into question the nature of Ypt/Rab specificity...
July 2014: Cellular Logistics
https://www.readbyqxmd.com/read/25610721/tgn-exit-of-the-cation-independent-mannose-6-phosphate-receptor-does-not-require-acid-hydrolase-binding
#18
Eline van Meel, Judith Klumperman
The cation-independent mannose 6-phosphate (Man-6-P) receptor (CI-MPR) binds newly synthesized, Man-6-P-containing lysosomal acid hydrolases in the trans-Golgi network (TGN) for clathrin-mediated transport to endosomes. It has remained unresolved, however, whether acid hydrolase binding is required for exit of the CI-MPR from the TGN. To address this question we used a B cell line derived from a Mucolipidosis type II (MLII)/I-cell disease patient. In MLII patients, acid hydrolases do not acquire the Man-6-P recognition marker and as a consequence do not bind to the CI-MPR...
July 2014: Cellular Logistics
https://www.readbyqxmd.com/read/25610720/akap350c-targets-to-mitochondria-via-a-novel-amphipathic-alpha-helical-domain
#19
Twila A Mason, James R Goldenring, Elena Kolobova
Mitochondria regulate metabolism and homeostasis within cells. Mitochondria are also very dynamic organelles, constantly undergoing fission and fusion. The importance of maintaining proper mitochondrial dynamics is evident in the various diseases associated with defects in these processes. Protein kinase A (PKA) is a key regulator of mitochondrial dynamics. PKA is spatially regulated by A-Kinase Anchoring Proteins (AKAPs). We completed cloning of a novel AKAP350 isoform, AKAP350C. Immunostaining for endogenous AKAP350C showed localization to mitochondria...
July 2014: Cellular Logistics
https://www.readbyqxmd.com/read/25610719/class-c-abc-transporters-and-saccharomyces-cerevisiae-vacuole-fusion
#20
Terry L Sasser, Rutilio A Fratti
Membrane fusion is carried out by core machinery that is conserved throughout eukaryotes. This is comprised of Rab GTPases and their effectors, and SNARE proteins, which together are sufficient to drive the fusion of reconstituted proteoliposomes. However, an outer layer of factors that are specific to individual trafficking pathways in vivo regulates the spatial and temporal occurrence of fusion. The homotypic fusion of Saccharomyces cerevisiae vacuolar lysosomes utilizes a growing set of factors to regulate the fusion machinery that include members of the ATP binding cassette (ABC) transporter family...
July 2014: Cellular Logistics
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