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Journal of Laboratory Physicians

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https://www.readbyqxmd.com/read/30078977/incubation-and-its-effect-on-leishman-stain
#1
Rateesh Sareen, Menka Kapil, G N Gupta
BACKGROUND: Leishman stain is the universal stain used in staining of peripheral blood smears all over the world. AIM: To study the effect of incubation of buffer solution, slide alone and slide & buffer both on standard Leishman staining. The staining at times causes difficulty particularly in rainy season when there is increased moisture. METHOD: The study comprised of twenty peripheral blood smears selected consecutively in batches of ten each for two successive days ...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078976/cytologic-diagnosis-of-unusual-large-multiple-cutaneous-myxomas-in-a-case-of-carney-complex
#2
Manjusha Karegar, Mrinal Sarwate, Kanchan Kothari, Amey Rojekar, Leena Naik
Cutaneous myxomas are rare benign neoplasms which are frequently associated with Carney complex (CNC). Although more than 500 cases of CNC are reported, there is no literature on cytologic diagnosis of Cutaneous myxomas. An 18-year-old male, with no significant family history, presented with multiple cutaneous swellings, largest measuring 15 cm on the right cheek. He also had spotty skin pigmentations, raised adrenocorticotropic hormone levels and recurrent cardiac myxomas. Fine-needle aspiration cytology from the right cheek and suprapubic swellings revealed paucicellular smears with abundant myxoid material in the background, admixed with fragments of spindle and stellate cells with bland nuclear morphology, and vascular proliferation in few fragments...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078975/primitive-neuroectodermal-tumor-of-the-kidney-a-rare-case-report
#3
Amrita Talwar, Manjari Kishore, Minakshi Bhardwaj, Devender Singh Chauhan
Renal primitive neuroectodermal tumor (PNET) is a rare primary renal neoplasm. Morphologically, it may mimic small blue round-cell tumor. Hence, histopathology in conjunction with immunohistochemistry plays a significant role in correctly diagnosing this malignancy. We report a case of PNET of kidney in a 30-year-old female with an extension to inferior vena cava, who succumbed following an aggressive course of illness.
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078974/comparisons-of-metabolite-profile-from-paired-serum-and-ethylenediaminetetraacetic-acid-plasma-samples-using-dry-chemistry-technology-an-emergency-department-perspective
#4
Lokesh Kumar Sharma, Deep Dutta, Neera Sharma, Bhaskar Thakur
BACKGROUND: No data is available evaluating the difference in serum versus plasma sample assay of commonly tested parameters in the emergency department, where the sample processing time can be significantly reduced if plasma is used for analysis instead of conventionally used serum. Hence, this study aimed to evaluate the differences in serum versus plasma sample estimation of commonly evaluated biochemical parameters using dry chemistry technology. MATERIALS AND METHODS: Paired blood samples were collected from a single venipuncture of 405 patients admitted to the emergency department...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078973/second-trimester-fetal-autopsy-a-morphological-study-with-prenatal-usg-correlations-and-clinical-implications
#5
Chaitra Venkataswamy, Umamaheswari Gurusamy, S Vidhya Lakshmi
OBJECTIVES: The objective of this study is to analyze the second-trimester fetal autopsies and to reemphasize the role of autopsy by comparing autopsy findings with prenatal ultrasound observations. MATERIALS AND METHODS: Retrospective analysis of second-trimester fetal autopsies over a period of 7.5 years (January 2009-June 2016). A standard protocol of autopsy procedure was followed, which included external examination with photography, X-ray, internal examination, and histopathological examination...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078972/bloodstream-infections-and-trends-of-antimicrobial-sensitivity-patterns-at-port-blair
#6
Amit Banik, Sanjeev H Bhat, Abhay Kumar, Agnijeet Palit, Kandregula Snehaa
PURPOSE: Bloodstream infection can range from inapparent bacteremia until fulminant septic shock with high mortality. Microorganisms present in circulating blood whether continuously, intermittently, or transiently are a threat to every organ in the body. Culture of blood is a vital tool to diagnose such infections. Drug susceptibility patterns help in rationalizing therapy. OBJECTIVE: The objective of this study was to perform bacteriological analysis and assess drug sensitivity patterns of isolates from blood stream infections...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078971/seroprevalence-of-hsv-1-and-2-in-hiv-infected-males-with-and-without-gud-study-from-a-tertiary-care-setting-of-india
#7
Arshi Munawwar, Somesh Gupta, Surendra Kumar Sharma, Sarman Singh
BACKGROUND: Herpes simplex virus (HSV) infection is associated with an increased risk of both human immune deficiency virus (HIV) transmission and acquisition. However, in India, literature on HSV infections in in HIV-infected males has been scarce. The present study was carried out to assess the seroprevalence of these viruses in HIV-infected males, so as to provide a baseline data from India and report on HSV associated GUD prevalence in HIV infected males. OBJECTIVE: The aim of the study was to estimate the seroprevalence of herpes simplex type 1 and 2 viruses in HIV-infected males with and without genital ulcers disease (GUD)...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078970/determination-of-antibacterial-activity-and-metabolite-profile-of-ruta-graveolens-against-streptococcus-mutans-and-streptococcus-sobrinus
#8
Hamzah Abdulrahman Salman, Sankarasetty Venkatesh, Ramasamy Senthilkumar, B S Gnanesh Kumar, Aamer Mousa Ali
BACKGROUND: Ruta graveolens is one of the most used phytomedicines. To date, there is no report of determining the bioactivity of R. graveolens against cariogenic causing bacteria ( Streptococcus mutans and Streptococcus sobrinus ). OBJECTIVE: The objective of the present study was to determine the antibacterial activity and metabolite profile of R. graveolens against S. mutans and S. sobrinus . MATERIALS AND METHODS: R. graveolens plant material was collected and processed in the month of February...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078969/systemic-inflammatory-response-markers-in-preeclampsia
#9
Ersin Çintesun, Feyza Nur Incesu Çintesun, Huriye Ezveci, Fikret Akyürek, Çetin Çelik
PURPOSE: Neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), platelet distribution width (PDW), red cell distribution width (RDW), mean platelet volume (MPV), and plateletcrit (PCT) are known as systemic inflammatory response markers. In this study, we aimed to evaluate changes in NLR, PLR, PDW, RDW, MPV, and PCT in preeclampsia (PE) and their use in predicting its severity. MATERIALS AND METHODS: This is a retrospective case-control study. The study comprised 64 control group (healthy pregnant females), 51 females with mild PE, and 13 with severe PE...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078968/association-of-intercellular-adhesion-gene-a-with-biofilm-formation-in-staphylococci-isolates-from-patients-with-conjunctivitis
#10
Taghreed H T Elkhashab, Lamiaa A Adel, Mona Saad Nour, Magda Mahran, Mai Elkaffas
BACKGROUND: There is a great negative impact of biofilm-mediated infection on patient health which necessitates the use of reliable methods for detecting biofilm producers. AIMS: This study was done to determine biofilm-producing ability and the presence of intercellular a dhesion g ene A in clinical staphylococcal isolates and to assess the reliability of two phenotypic methods used for biofilm detection. MATERIALS AND METHODS: Fifty staphylococcal strains were isolated from 100 conjunctival swabs from patients attended the Ophthalmology Outpatient Department of the Research Institute of Ophthalmology...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078967/evaluation-of-impact-of-occult-hepatitis-b-infection-in-chronic-hcv-infected-patients-a-retrospective-cohort-study
#11
Mohit Bhatia, Ekta Gupta, Manish C Choudhary, Ankur Jindal, Shiv Kumar Sarin
CONTEXT: Occult hepatitis B infection (OBI) may contribute to liver damage and variable therapeutic response in patients with chronic hepatitis C (CHC) infection. AIMS: To study the prevalence of OBI and to evaluate its impact and/or that of anti-HBc total seropositivity on clinical outcomes and response to directly acting antiviral (DAA) therapy in CHC-infected patients. SETTINGS AND DESIGN: A retrospective cohort study was conducted in a tertiary care liver hospital from January to May 2017...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078966/direct-identification-and-susceptibility-testing-of-gram-negative-bacilli-from-turbid-urine-samples-using-vitek2
#12
Neelima Angaali, Laxmi Vemu, Chavali Padmasri, Neeraja Mamidi, Vijay Dharma Teja
INTRODUCTION: Urinary tract infections (UTIs) are the most common infectious diseases occurring in either the community or healthcare setting. Turnaround time for urine culture is about 24 h, and antimicrobial susceptibility testing (AST) requires another 24 h. Consequently, initial antibiotic therapy is mostly empirical. MATERIALS AND METHODS: This study was conducted at Nizam's Institute of Medical Sciences, Hyderabad. Turbid urine samples which showed pus cells and Gram-negative (GN) bacilli of single morphotype were included...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078965/importance-of-combined-approach-of-investigations-for-detection-of-asymptomatic-hashimoto-thyroiditis-in-early-stage
#13
Sonali Rajesh Saraf, Nitin M Gadgil, Sheetal Yadav, Alka D Kalgutkar
INTRODUCTION: Hashimoto's thyroiditis (HT) is the most common cause of goitrous hypothyroidism in iodine sufficient areas. The diagnosis of HT is important because it progresses to hypothyroidism, and also, it is associated with thyroid lymphoma and papillary thyroid carcinoma. Apart from thyroid antibodies, assessment of thyroid function test (TFT) levels, ultrasonography (USG), and cytological analysis can help in early diagnosis and management of HT. AIMS AND OBJECTIVES OF THIS STUDY: To know the age incidence and clinical presentation of HT, its association with thyroid hormone levels and with thyroid antibodies and its correlation with the cytologic grade of HT...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078964/clinical-and-laboratory-standards-institute-versus-european-committee-for-antimicrobial-susceptibility-testing-guidelines-for-interpretation-of-carbapenem-antimicrobial-susceptibility-results-for-escherichia-coli-in-urinary-tract-infection-uti
#14
Chinmoy Sahu, Vidhi Jain, Prabhakar Mishra, Kashi Nath Prasad
BACKGROUND: Carbapenems show excellent activity against resistant uropathogens, and they are the antibiotics of choice for urinary tract infections (UTIs). The choice of carbapenem prescription is strongly influenced by antimicrobial susceptibility testing (AST) report. With the publication of recent AST guidelines by the European Committee on AST (EUCAST), we were curious to evaluate the difference in results between Clinical and Laboratory Standards Institute (CLSI) and the EUCAST guidelines for the interpretation of carbapenems...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078963/occurrence-and-characterization-of-hyperviscous-k1-and-k2-serotype-in-klebsiella-pneumoniae
#15
Poothakuzhiyil Remya, Mariappan Shanthi, Uma Sekar
BACKGROUND: Klebsiella pneumoniae causes both nosocomial and community-associated infections. Hypervirulent K. pneumoniae (hvKP), new variant of K. pneumoniae , can cause invasive infections in young healthy individuals as well as in the immunocompromised population. Hypervirulent strains frequently belong to capsular serotypes K1 or K2. Emergence of antimicrobial resistance in hvKP is a cause for concern. AIM AND OBJECTIVE: The present study was done to detect the K1 and K2 serotypes among clinical isolates of K...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078962/concept-and-connotation-of-oxidative-stress-in-preeclampsia
#16
Hayder M Al-Kuraishy, Ali I Al-Gareeb, Thabat J Al-Maiahy
BACKGROUND: Preeclampsia (PE) is a systemic pregnancy-related disorder characterized by hypertension, proteinuria, and edema. Free radicals seem to play an important role in the induction of endothelial dysfunction in PE. AIM: The aim of the present study was to investigate serum levels of nitric oxide (NO), peroxynitrite (ONOO- ), paraoxonase (PON-1), malondialdehyde (MDA), and lipid profile in preeclamptic patients compared to the women with normal pregnancy. MATERIALS AND METHODS: A total of 68 pregnant women were recruited...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078961/rosuvastatin-as-forthcoming-antibiotic-or-as-adjuvant-additive-agent-in-vitro-novel-antibacterial-study
#17
Hayder M Al-Kuraishy, Ali I Al-Gareeb, Ali K Al-Buhadily
INTRODUCTION: Rosuvastatin is a lipid-lowering agent that inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase leading to a reduction of cholesterol biosynthesis. Many studies have shown an association between statins use and the reduction of sepsis. The aim of the present study was to evaluate the in vitro combined antibacterial activity of rosuvastatin and cefixime. MATERIALS AND METHODS: Five pathogenic bacteria isolates (Gram positive and Gram negative) were used for testing the antibacterial activity of rosuvastatin alone and in combination with cefixime...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078960/evaluation-of-antibacterial-properties-of-hydroxyapatite-bioactive-glass-and-fluorapatite-bioactive-glass-nanocomposite-foams-as-a-cellular-scaffold-of-bone-tissue
#18
Seyedali Seyedmajidi, Ramazan Rajabnia, Maryam Seyedmajidi
AIMS AND OBJECTIVES: Infection is a serious problem for patients after implantation surgery, which is difficult to treat with antibiotic therapy. The present study was developed to evaluate and compare the antibacterial properties of hydroxyapatite/bioactive glass (HA/BG) and fluorapatite/bioactive glass (FA/BG) nanocomposite foams as a cellular scaffold for use in bone defects by two macrodilution and disk diffusion methods. MATERIALS AND METHODS: Staphylococcus aureus, Enterococcus faecalis , and Streptococcus mutans were cultured in brain heart infusion broth medium with nanocomposite powder for 5 days, and their bioactivity levels were evaluated by daily culturing on solid agar medium plates...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078959/direct-testing-by-vitek-%C3%A2-2-a-dependable-method-to-reduce-turnaround-time-in-gram-negative-bloodstream-infections
#19
Purabi Barman, Shimpi Chopra, Tarun Thukral
CONTEXT: Bloodstream infections pose a major health-care burden worldwide. Routine microbiological methods are time-consuming, thereby delaying appropriate treatment. AIMS: The aim of this study is to evaluate the method of direct testing of identification (ID) and antimicrobial susceptibility testing (AST) of positive blood culture bottles by VITEK® 2. SETTINGS AND DESIGN: This was a prospective study at a tertiary level hospital. SUBJECTS AND METHODS: One hundred positive BACTEC blood culture bottles with monomicrobial Gram-negative organisms on microscopy were tested in parallel by direct ID/AST as well as conventional method...
July 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/30078958/spectrum-of-candidal-species-isolated-from-neonates-admitted-in-an-intensive-care-unit-of-teaching-hospital-of-kashmir-north-india
#20
Asifa Nazir, Talat Masoodi
BACKGROUND: Candidal infections are an important cause of morbidity and mortality in Neonatal Intensive Care Unit. Neonatal candidiasis is increasing in frequency, mainly because of increase in the survival of babies with low-birth weight, preterm births, advancement in medical field, life support systems, relative immunodeficiency, and extensive use of broad-spectrum antibiotics. Over the past few decades, there has been a progressive shift from the predominance of Candida albicans to nonalbicans Candida species...
July 2018: Journal of Laboratory Physicians
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