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Journal of Tissue Engineering

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https://www.readbyqxmd.com/read/29118967/cold-perfusion-decellularization-of-whole-organ-porcine-pancreas-supports-human-fetal-pancreatic-cell-attachment-and-expression-of-endocrine-and-exocrine-markers
#1
Erik Elebring, Vijay K Kuna, Niclas Kvarnström, Suchitra Sumitran-Holgersson
Despite progress in the field of decellularization and recellularization, the outcome for pancreas has not been adequate. This might be due to the challenging dual nature of pancreas with both endocrine and exocrine tissues. We aimed to develop a novel and efficient cold-perfusion method for decellularization of porcine pancreas and recellularize acellular scaffolds with human fetal pancreatic stem cells. Decellularization of whole porcine pancreas at 4°C with sodium deoxycholate, Triton X-100 and DNase efficiently removed cellular material, while preserving the extracellular matrix structure...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29081966/design-of-biomimetic-cellular-scaffolds-for-co-culture-system-and-their-application
#2
Yun-Min Kook, Yoon Jeong, Kangwon Lee, Won-Gun Koh
The extracellular matrix of most natural tissues comprises various types of cells, including fibroblasts, stem cells, and endothelial cells, which communicate with each other directly or indirectly to regulate matrix production and cell functionality. To engineer multicellular interactions in vitro, co-culture systems have achieved tremendous success achieving a more realistic microenvironment of in vivo metabolism than monoculture system in the past several decades. Recently, the fields of tissue engineering and regenerative medicine have primarily focused on three-dimensional co-culture systems using cellular scaffolds, because of their physical and biological relevance to the extracellular matrix of actual tissues...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29051810/controlled-dexamethasone-delivery-via-double-walled-microspheres-to-enhance-long-term-adipose-tissue-retention
#3
Arta Kelmendi-Doko, J Peter Rubin, Katarina Klett, Christopher Mahoney, Sheri Wang, Kacey G Marra
Current materials used for adipose tissue reconstruction have critical shortcomings such as suboptimal volume retention, donor-site morbidity, and poor biocompatibility. The aim of this study was to examine a controlled delivery system of dexamethasone to generate stable adipose tissue when mixed with disaggregated human fat in an athymic mouse model for 6 months. The hypothesis that the continued release of dexamethasone from polymeric microspheres would enhance both adipogenesis and angiogenesis more significantly when compared to the single-walled microsphere model, resulting in long-term adipose volume retention, was tested...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29051809/impact-of-expansion-and-redifferentiation-under-hypothermia-on-chondrogenic-capacity-of-cultured-human-septal-chondrocytes
#4
Achim von Bomhard, Joseph Faust, Alexander F Elsaesser, Silke Schwarz, Katharina Pippich, Nicole Rotter
A critical limitation in the cultivation of cartilage for tissue engineering is the dedifferentiation in chondrocytes, mainly during in vitro amplification. Despite many previous studies investigating the influence of various conditions, no data exist concerning the effects of hypothermia. Our aim has been to influence chondrocyte dedifferentiation in vitro by hypothermic conditions. Chondrocytes were isolated from cartilage biopsies and seeded in monolayer and in three-dimensional pellet-cultures. Each cell culture was either performed at 32...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29051808/making-microenvironments-a-look-into-incorporating-macromolecular-crowding-into-in-vitro-experiments-to-generate-biomimetic-microenvironments-which-are-capable-of-directing-cell-function-for-tissue-engineering-applications
#5
Paula Benny, Michael Raghunath
Biomimetic microenvironments are key components to successful cell culture and tissue engineering in vitro. One of the most accurate biomimetic microenvironments is that made by the cells themselves. Cell-made microenvironments are most similar to the in vivo state as they are cell-specific and produced by the actual cells which reside in that specific microenvironment. However, cell-made microenvironments have been challenging to re-create in vitro due to the lack of extracellular matrix composition, volume and complexity which are required...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28989698/recent-advances-to-accelerate-re-endothelialization-for-vascular-stents
#6
Tarek M Bedair, Mahmoud A ElNaggar, Yoon Ki Joung, Dong Keun Han
Cardiovascular diseases are considered as one of the serious diseases that leads to the death of millions of people all over the world. Stent implantation has been approved as an easy and promising way to treat cardiovascular diseases. However, in-stent restenosis and thrombosis remain serious problems after stent implantation. It was demonstrated in a large body of previously published literature that endothelium impairment represents a major factor for restenosis. This discovery became the driving force for many studies trying to achieve an optimized methodology for accelerated re-endothelialization to prevent restenosis...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28912946/crosslinking-method-of-hyaluronic-based-hydrogel-for-biomedical-applications
#7
Sureerat Khunmanee, Younghyen Jeong, Hansoo Park
In the field of tissue engineering, there is a need for advancement beyond conventional scaffolds and preformed hydrogels. Injectable hydrogels have gained wider admiration among researchers as they can be used in minimally invasive surgical procedures. Injectable gels completely fill the defect area and have good permeability and hence are promising biomaterials. The technique can be effectively applied to deliver a wide range of bioactive agents, such as drugs, proteins, growth factors, and even living cells...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28890781/thermo-responsive-cell-culture-carrier-effects-on-macrophage-functionality-and-detachment-efficiency
#8
Knut Rennert, Mirko Nitschke, Maria Wallert, Natalie Keune, Martin Raasch, Stefan Lorkowski, Alexander S Mosig
Harvesting cultivated macrophages for tissue engineering purposes by enzymatic digestion of cell adhesion molecules can potentially result in unintended activation, altered function, or behavior of these cells. Thermo-responsive polymer is a promising tool that allows for gentle macrophage detachment without artificial activation prior to subculture within engineered tissue constructs. We therefore characterized different species of thermo-responsive polymers for their suitability as cell substrate and to mediate gentle macrophage detachment by temperature shift...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28890780/recellularization-of-decellularized-heart-valves-progress-toward-the-tissue-engineered-heart-valve
#9
REVIEW
Mitchell C VeDepo, Michael S Detamore, Richard A Hopkins, Gabriel L Converse
The tissue-engineered heart valve portends a new era in the field of valve replacement. Decellularized heart valves are of great interest as a scaffold for the tissue-engineered heart valve due to their naturally bioactive composition, clinical relevance as a stand-alone implant, and partial recellularization in vivo. However, a significant challenge remains in realizing the tissue-engineered heart valve: assuring consistent recellularization of the entire valve leaflets by phenotypically appropriate cells...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28890779/elements-of-the-niche-for-adult-stem-cell-expansion
#10
Patricia A Redondo, Marina Pavlou, Marilena Loizidou, Umber Cheema
Adult stem cells are crucial for tissue homeostasis. These cells reside within exclusive locations in tissues, termed niches, which protect adult stem cell fidelity and regulate their many functions through biophysical-, biochemical- and cellular-mediated mechanisms. There is a growing understanding of how these mechanisms and their components contribute towards maintaining stem cell quiescence, self-renewal, expansion and differentiation patterns. In vitro expansion of adult stem cells is a powerful tool for understanding stem cell biology, and for tissue engineering and regenerative medicine applications...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28835816/a-quantitative-multi-national-and-multi-stakeholder-assessment-of-barriers-to-the-adoption-of-cell-therapies
#11
Benjamin M Davies, James Smith, Sarah Rikabi, Karolina Wartolowska, Mark Morrey, Anna French, Robert MacLaren, David Williams, Kim Bure, Rafael Pinedo-Villanueva, Anthony Mathur, Martin Birchall, Evan Snyder, Anthony Atala, Brock Reeve, David Brindley
Cellular therapies, such as stem cell-based treatments, have been widely researched and numerous products and treatments have been developed. Despite this, there has been relatively limited use of these technologies in the healthcare sector. This study sought to investigate the perceived barriers to this more widespread adoption. An anonymous online questionnaire was developed, based on the findings of a pilot study. This was distributed to an audience of clinicians, researchers and commercial experts in 13 countries...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28815010/development-and-characterisation-of-a-low-concentration-sodium-dodecyl-sulphate-decellularised-porcine-dermis
#12
Jack A Helliwell, Daniel S Thomas, Vaia Papathanasiou, Shervanthi Homer-Vanniasinkam, Amisha Desai, Louise M Jennings, Paul Rooney, John N Kearney, Eileen Ingham
The aim of this study was to adapt a proprietary decellularisation process for human dermis for use with porcine skin. Porcine skin was subject to: sodium chloride (1 M) to detach the epidermis, trypsin paste to remove hair follicles, peracetic acid (0.1% v/v) disinfection, washed in hypotonic buffer and 0.1% (w/v) sodium dodecyl sulphate in the presence of proteinase inhibitors followed by nuclease treatment. Cellular porcine skin, decellularised porcine and human dermis were compared using histology, immunohistochemistry, GSL-1 lectin (alpha-gal epitope) staining, biochemical assays, uniaxial tensile and in vitro cytotoxicity tests...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28794849/scaffolds-for-retinal-pigment-epithelial-cell-transplantation-in-age-related-macular-degeneration
#13
REVIEW
Corina E White, Ronke M Olabisi
In several retinal degenerative diseases, including age-related macular degeneration, the retinal pigment epithelium, a highly functionalized cell monolayer, becomes dysfunctional. These retinal diseases are marked by early retinal pigment epithelium dysfunction reducing its ability to maintain a healthy retina, hence making the retinal pigment epithelium an attractive target for treatment. Cell therapies, including bolus cell injections, have been investigated with mixed results. Since bolus cell injection does not promote the proper monolayer architecture, scaffolds seeded with retinal pigment epithelium cells and then implanted have been increasingly investigated...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28794848/bioactive-calcium-phosphate-based-glasses-and-ceramics-and-their-biomedical-applications-a-review
#14
Md Towhidul Islam, Reda M Felfel, Ensanya A Abou Neel, David M Grant, Ifty Ahmed, Kazi M Zakir Hossain
An overview of the formation of calcium phosphate under in vitro environment on the surface of a range of bioactive materials (e.g. from silicate, borate, and phosphate glasses, glass-ceramics, bioceramics to metals) based on recent literature is presented in this review. The mechanism of bone-like calcium phosphate (i.e. hydroxyapatite) formation and the test protocols that are either already in use or currently being investigated for the evaluation of the bioactivity of biomaterials are discussed. This review also highlights the effect of chemical composition and surface charge of materials, types of medium (e...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28758007/re-epithelialization-of-whole-porcine-kidneys-with-renal-epithelial-cells
#15
Nafiseh Poornejad, Evan Buckmiller, Lara Schaumann, Haonan Wang, Jonathan Wisco, Beverly Roeder, Paul Reynolds, Alonzo Cook
Decellularized porcine kidneys were recellularized with renal epithelial cells by three methods: perfusion through the vasculature under high pressure, perfusion through the ureter under high pressure, or perfusion through the ureter under moderate vacuum. Histology, scanning electron microscopy, confocal microscopy, and magnetic resonance imaging were used to assess vasculature preservation and the distribution of cells throughout the kidneys. Cells were detected in the magnetic resonance imaging by labeling them with iron oxide...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28717506/injectable-scaffold-materials-differ-in-their-cell-instructive-effects-on-primary-human-myoblasts
#16
Eva Kildall Hejbøl, Jeeva Sellathurai, Prabha Damodaran Nair, Henrik Daa Schrøder
Scaffolds are materials used for delivery of cells for regeneration of tissues. They support three-dimensional organization and improve cell survival. For the repair of small skeletal muscles, injections of small volumes of cells are attractive, and injectable scaffolds for delivery of cells offer a minimally invasive technique. In this study, we examined in vitro the cell instructive effects of three types of injectable scaffolds, fibrin, alginate, and poly(lactic-co-glycolic acid)-based microparticles on primary human myoblasts...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28634532/cellularizing-hydrogel-based-scaffolds-to-repair-bone-tissue-how-to-create-a-physiologically-relevant-micro-environment
#17
Mathieu Maisani, Daniele Pezzoli, Olivier Chassande, Diego Mantovani
Tissue engineering is a promising alternative to autografts or allografts for the regeneration of large bone defects. Cell-free biomaterials with different degrees of sophistication can be used for several therapeutic indications, to stimulate bone repair by the host tissue. However, when osteoprogenitors are not available in the damaged tissue, exogenous cells with an osteoblast differentiation potential must be provided. These cells should have the capacity to colonize the defect and to participate in the building of new bone tissue...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28616152/magnetically-levitated-mesenchymal-stem-cell-spheroids-cultured-with-a-collagen-gel-maintain-phenotype-and-quiescence
#18
Natasha S Lewis, Emily El Lewis, Margaret Mullin, Helen Wheadon, Matthew J Dalby, Catherine C Berry
Multicellular spheroids are an established system for three-dimensional cell culture. Spheroids are typically generated using hanging drop or non-adherent culture; however, an emerging technique is to use magnetic levitation. Herein, mesenchymal stem cell spheroids were generated using magnetic nanoparticles and subsequently cultured within a type I collagen gel, with a view towards developing a bone marrow niche environment. Cells were loaded with magnetic nanoparticles, and suspended beneath an external magnet, inducing self-assembly of multicellular spheroids...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28616151/therapeutic-potential-of-dental-stem-cells
#19
REVIEW
Elna Paul Chalisserry, Seung Yun Nam, Sang Hyug Park, Sukumaran Anil
Stem cell biology has become an important field in regenerative medicine and tissue engineering therapy since the discovery and characterization of mesenchymal stem cells. Stem cell populations have also been isolated from human dental tissues, including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, dental follicle progenitor cells, and periodontal ligament stem cells. Dental stem cells are relatively easily obtainable and exhibit high plasticity and multipotential capabilities...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28567273/bone-and-cartilage-differentiation-of-a-single-stem-cell-population-driven-by-material-interface
#20
Hannah Donnelly, Carol-Anne Smith, Paula E Sweeten, Nikolaj Gadegaard, Rm Dominic Meek, Matteo D'Este, Alvaro Mata, David Eglin, Matthew J Dalby
Adult stem cells, such as mesenchymal stem cells, are a multipotent cell source able to differentiate towards multiple cell types. While used widely in tissue engineering and biomaterials research, they present inherent donor variability and functionalities. In addition, their potential to form multiple tissues is rarely exploited. Here, we combine an osteogenic nanotopography and a chondrogenic hyaluronan hydrogel with the hypothesis that we can make a complex tissue from a single multipotent cell source with the exemplar of creating a three-dimensional bone-cartilage boundary environment...
January 2017: Journal of Tissue Engineering
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