journal
MENU ▼
Read by QxMD icon Read
search

Cell Medicine

journal
https://www.readbyqxmd.com/read/26858909/fluorescence-quenching-of-cdse-zns-quantum-dots-by-using-black-hole-quencher-molecules-intermediated-with-peptide-for-biosensing-application
#1
Sreenadh Sasidharan Pillai, Hiroshi Yukawa, Daisuke Onoshima, Vasudevanpillai Biju, Yoshinobu Baba
Quantum dots (QDs) have recently been investigated as fluorescent probes for detecting a very small number of biomolecules and live cells; however, the establishment of molecular imaging technology with on-off control of QD fluorescence remains to be established. Here we have achieved the fluorescence off state of QDs with the conjugation of black hole quencher (BHQ) molecules intermediated with peptide by using streptavidin-QDs585 and biotin-pep-BHQ-1. The fluorescence of streptavidin-QDs585 was decreased by the addition of biotin-pep-BHQ-1 in a dose-dependent manner...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858908/spheroid-formation-and-evaluation-of-hepatic-cells-in-a-three-dimensional-culture-device
#2
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi
In drug discovery, it is very important to evaluate liver cells within an organism. Compared to 2D culture methods, the development of 3D culture techniques for liver cells has been successful in maintaining long-term liver functionality with the formation of a hepatic-specific structure. The key to performing drug testing is the establishment of a stable in vitro evaluation system. In this article, we report a Tapered Stencil for Cluster Culture (TASCL) device developed to create liver spheroids in vitro. The TASCL device will be applied as a toxicity evaluation system for drug discovery...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858907/human-laminin-isotype-coating-for-creating-islet-cell-sheets
#3
Shingo Yamashita, Kazuo Ohashi, Rie Utoh, Teruo Okano, Masakazu Yamamoto
Our experimental approach toward the development of new islet-based treatment for diabetes mellitus has been the creation of a monolayered islet cell construct (islet cell sheet), followed by its transplantation into a subcutaneous pocket. Previous studies describe rat laminin-5 (chain composition: α3, β3, γ2) as a suitable extracellular matrix (ECM) for surfaces comprised of a coated temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm). To progress toward the clinical application of this approach, the present study attempted to identify an optimal human ECM as a coating material on PIPAAm surfaces, which allowed islet cells to attach on the surfaces and subsequently to be harvested as a monolithic cell sheet...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858906/efficient-gene-transduction-of-dispersed-islet-cells-in-culture-using-fiber-modified-adenoviral-vectors
#4
Hiroyuki Hanayama, Kazuo Ohashi, Rie Utoh, Hirofumi Shimizu, Kazuya Ise, Fuminori Sakurai, Hiroyuki Mizuguchi, Hiroyuki Tsuchiya, Teruo Okano, Mitsukazu Gotoh
To establish novel islet-based therapies, our group has recently developed technologies for creating functional neo-islet tissues in the subcutaneous space by transplanting monolithic sheets of dispersed islet cells (islet cell sheets). Improving cellular function and viability are the next important challenges for enhancing the therapeutic effects. This article describes the adenoviral vector-mediated gene transduction of dispersed islet cells under culture conditions. Purified pancreatic islets were obtained from Lewis rats and dissociated into single islet cells...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858905/islet-culture-preservation-before-islet-transplantation
#5
REVIEW
Hirofumi Noguchi, Chika Miyagi-Shiohira, Kiyoto Kurima, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita
Although islet culture prior to transplantation provides flexibility for the evaluation of isolated islets and the pretreatment of patients, it is well known that isolated islets deteriorate rapidly in culture. Human serum albumin (HSA) is used for medium supplementation instead of fetal bovine serum (FBS), which is typically used for islet culture research, to avoid the introduction of xenogeneic materials. However, FBS contains several factors that are beneficial to islet viability and which also neutralize the endogenous pancreatic enzymes or exogenous enzymes left over from the isolation process...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858904/choice-of-feeders-is-important-when-first-establishing-ipscs-derived-from-primarily-cultured-human-deciduous-tooth-dental-pulp-cells
#6
Issei Saitoh, Emi Inada, Yoko Iwase, Hirofumi Noguchi, Tomoya Murakami, Miki Soda, Naoko Kubota, Hiroko Hasegawa, Eri Akasaka, Yuko Matsumoto, Kyoko Oka, Youichi Yamasaki, Haruaki Hayasaki, Masahiro Sato
Feeder cells are generally required to maintain embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs). Mouse embryonic fibroblasts (MEFs) isolated from fetuses and STO mouse stromal cell line are the most widely used feeder cells. The aim of this study was to determine which cells are suitable for establishing iPSCs from human deciduous tooth dental pulp cells (HDDPCs). Primary cultures of HDDPCs were cotransfected with three plasmids containing human OCT3/4, SOX2/KLF4, or LMYC/LIN28 and pmaxGFP by using a novel electroporation method, and then cultured in an ESC qualified medium for 15 days...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858903/cryopreservation-of-adipose-derived-mesenchymal-stem-cells
#7
REVIEW
Chika Miyagi-Shiohira, Kiyoto Kurima, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi
Mesenchymal stem cells (MSCs) have the potential to differentiate into cells of mesodermal origin such as osteoblasts, adipocytes, myocytes, and chondrocytes. They possess an immunosuppressive effect, which makes them a viable cell population for the cell-based therapy of treatment-resistant immune diseases. Adipose-derived mesenchymal stem cells (ASCs) have been demonstrated to have the ability to acquire the properties of subcutaneous adipose tissue particularly easily, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858902/creating-a-future-of-transplantation
#8
Hirofumi Noguchi
No abstract text is available yet for this article.
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858901/disease-and-stem-cell-based-analysis-of-the-2014-asntr-meeting
#9
David J Eve
A wide variety of subjects are presented at the annual American Society of Neural Therapy and Repair meeting every year, as typified by this summary of the 2014 meeting. Parkinson's disease-related presentations were again the most popular topic, with traumatic brain injury, spinal cord injury, and stroke being close behind. Other disorders included Huntington's disease, brain cancer, and bipolar disorders. Several studies were related to multiple diseases, and many studies attempted to reveal more about the disease process...
October 1, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858900/a-combination-of-low-intensity-pulsed-ultrasound-and-nanohydroxyapatite-concordantly-enhances-osteogenesis-of-adipose-derived-stem-cells-from-buccal-fat-pad
#10
Rika Nagasaki, Yoshiki Mukudai, Yasumasa Yoshizawa, Masahiro Nagasaki, Sunao Shiogama, Maiko Suzuki, Seiji Kondo, Satoru Shintani, Tatsuo Shirota
The osteogenic induction of adipose-derived stem cells (ADSCs) has been regarded as an important step in bone tissue engineering. In the present study, we focused on the buccal fat pad (BFP) as a source of adipose tissue, since BFPs are encapsulated by adipose tissue and are often coextirpated during oral surgery. Low-intensity pulsed ultrasound (LIPUS) is effective in the treatment of fractures, and nanohydroxyapatite (NHA) is known as a bone substitute material. Here we investigated the synergistic effects of LIPUS and NHA in the osteogenesis of ADSCs...
October 1, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858899/a-rotating-bioreactor-for-scalable-culture-and-differentiation-of-respiratory-epithelium
#11
Micha Sam Brickman Raredon, Mahboobe Ghaedi, Elizabeth A Calle, Laura E Niklason
Respiratory epithelium is difficult to grow in vitro, as it requires a well-maintained polarizing air-liquid interface (ALI) to maintain differentiation. Traditional methods rely on permeable membrane culture inserts, which are difficult to work with and are ill-suited for the production of large numbers of cells, such as the quantities required for cell-based clinical therapies. Herein, we investigate an alternative form of culture in which the cells are placed on a porous substrate that is continuously rolled, such that the monolayer of cells is alternately submerged in media or apically exposed to air...
October 1, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858898/immature-dental-pulp-stem-cells-showed-renotropic-and-pericyte-like-properties-in-acute-renal-failure-in-rats
#12
Michele A Barros, João Flávio Panattoni Martins, Durvanei Augusto Maria, Crisitiane Valverde Wenceslau, Dener Madeiro De Souza, Alexandre Kerkis, Niels Olsen S Câmara, Julio Cesar C Balieiro, Irina Kerkis
Acute renal failure (ARF) is a common renal disease that can lead to high mortality. Recovery from ARF occurs with the replacement of necrotic tubular cells by functional tubular epithelial cells and the normalization of microvascular endothelial cell function in the peritubular capillaries. Conventional therapeutic techniques are often ineffective against ARF. Hence, stem cell therapies, which act through multiple trophic and regenerative mechanisms, are encouraging. We investigated the homing of human immature dental pulp stem cells (IDPSCs) after endovenous (EV) or intraperitoneal (IP) injection, in immunocompetent Wistar rats with ARF induced by intramuscular injection of glycerol, without the use of immunosuppression...
October 1, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858897/potential-factors-for-the-differentiation-of-escs-ipscs-into-insulin-producing-cells
#13
Takako Tsugata, Naruo Nikoh, Tatsuya Kin, Issei Saitoh, Yasufumi Noguchi, Hideo Ueki, Masami Watanabe, Andrew M James Shapiro, Hirofumi Noguchi
The low efficiency of in vitro differentiation of human embryonic stem cells (ESCs) or human induced pluripotent stem cells (iPSCs) into insulin-producing cells thus creates a crucial hurdle for the clinical implementation of human pluripotent stem cells (PSCs). In this study, we investigated the key factors for the differentiation of PSCs into insulin-producing cells. We obtained microarray data of HUES8 and HUES6 from two GeneChips (GPL3921: Affymetrix HT Human Genome U133A Array, GPL570: Affymetrix Human Genome U133 Plus 2...
February 8, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858896/influence-of-autofluorescence-derived-from-living-body-on-in-vivo-fluorescence-imaging-using-quantum-dots
#14
Hiroshi Yukawa, Masaki Watanabe, Noritada Kaji, Yoshinobu Baba
Quantum dots (QDs) are thought to be a novel inorganic probe for in vivo fluorescence imaging because of their excellent fluorescence properties. Autofluorescence is generally known to be produced from various living bodies including humans, rats, and mice. However, the influence of the autofluorescence on in vivo fluorescence imaging using QDs remains poorly understood. In this article, we assessed the autofluorescence derived from a mouse body and the influence of the autofluorescence on in vivo fluorescence imaging using QDs...
February 8, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858895/three-dimensional-in-vitro-hepatic-constructs-formed-using-combinatorial-tapered-stencil-for-cluster-culture-tascl-device
#15
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi
Attempts to create artificial liver tissue from various cells have been reported as an alternative method for liver transplantation and pharmaceutical testing. In the construction of artificial liver tissue, the selection of the cell source is the most important factor. However, if an appropriate environment (in vitro/in vivo) cannot be provided for various cells, it is not possible to obtain artificial liver tissue with the desired function. Therefore, we focused on the in vitro environment and produced liver tissues using MEMS technology...
February 8, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858894/improvement-of-infusion-process-in-cell-transplantation-effect-of-shear-stress-on-hepatocyte-viability-under-horizontal-and-vertical-syringe-orientation
#16
Sandi Sufiandi, Hiromichi Obara, Shin Enosawa, Huai-Che Hsu, Naoto Matsuno, Hiroshi Mizunuma
Improving cell viability and function are important for enhancing the clinical results of cell transplantation. The relationship between cell viability and shear stress remains unexplained, and sedimentation effects during the infusion process are important to the hepatocyte transplantation process. In the present study, the relationship between cell viability and shear stress in the presence of sedimentation effect was investigated using a microchannel simulating the cell transplantation process under several shear stress conditions...
February 8, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858893/synergistic-effects-of-calcineurin-inhibitors-and-steroids-on-steroid-sensitivity-of-peripheral-blood-mononuclear-cells
#17
Hironori Takeuchi, Hitoshi Iwamoto, Yuki Nakamura, Toshihiko Hirano, Osamu Konno, Yu Kihara, Naokazu Chiba, Takayoshi Yokoyama, Kiminori Takano, Tatsunori Toraishi, Kiyoshi Okuyama, Chie Ikeda, Sachiko Tanaka, Kenji Onda, Akiko Soga, Yukiko Kikuchi, Takashi Kawaguchi, Shigeyuki Kawachi, Sakae Unezaki, Motohide Shimazu
The steroid receptor (SR) complex contains FKBP51 and FKBP52, which bind to tacrolimus (TAC) and cyclophilin 40, which, in turn, bind to cyclosporine (CYA); these influence the intranuclear mobility of steroid-SR complexes. Pharmacodynamic interactions are thought to exist between steroids and calcineurin inhibitors (CNIs) on the SR complex. We examined the effect of CNIs on steroid sensitivity. Methylprednisolone (MPSL) sensitivity was estimated as the concentration inhibiting mitosis in 50% (IC50) of peripheral blood mononuclear cells and as the area under the MPSL concentration-proliferation suppressive rate curves (CPS-AUC) in 30 healthy subjects...
February 8, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858892/basic-and-clinical-science-for-organ-biology
#18
Hirofumi Noguchi
No abstract text is available yet for this article.
February 8, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858891/preculturing-islets-with-adipose-derived-mesenchymal-stromal-cells-is-an-effective-strategy-for-improving-transplantation-efficiency-at-the-clinically-preferred-intraportal-site
#19
Chloe L Rackham, Paramjeet K Dhadda, Aurélie M Le Lay, Aileen J F King, Peter M Jones
We have recently shown that preculturing islets with kidney-derived mesenchymal stromal cells (MSCs) improves transplantation outcome in streptozotocin-diabetic mice implanted with a minimal mass of islets beneath the kidney capsule. In the present study, we have extended our previous observations to investigate whether preculturing islets with MSCs can also be used to enhance islet function at the clinically used intraportal site. We have used MSCs derived from adipose tissue, which are more readily accessible than alternative sources in human subjects and can be expanded to clinically efficacious numbers, to preculture islets throughout this study...
November 5, 2014: Cell Medicine
https://www.readbyqxmd.com/read/26858890/human-menstrual-blood-derived-mesenchymal-cells-as-new-human-feeder-layer-system-for-human-embryonic-stem-cells
#20
Danúbia Silva Dos Santos, Vanessa Carvalho Coelho de Oliveira, Karina Dutra Asensi, Leandro Vairo, Adriana Bastos Carvalho, Antonio Carlos Campos de Carvalho, Regina Coeli Dos Santos Goldenberg
Human embryonic stem cells (hESCs) in general require coculture with feeder layers in order to remain undifferentiated. However, the use of animal-derived feeder layers is incompatible with the clinical setting. The objective of this work was to investigate whether human menstrual blood-derived mesenchymal cells (MBMCs) can substitute mouse embryonic fibroblasts (MEFs) as a feeder layer for H9-hESCs. Both feeder cell types were isolated and cultured in DMEM F-12 and high glucose DMEM, respectively. After three passages, they were inactivated with mitomycin C...
November 5, 2014: Cell Medicine
journal
journal
43308
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"