journal
MENU ▼
Read by QxMD icon Read
search

Cell Medicine

journal
https://www.readbyqxmd.com/read/28174675/comparison-of-purification-solutions-with-different-osmolality-for-porcine-islet-purification
#1
Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi
The osmolality of the purification solution is one of the most critical variables in human islet purification during islet isolation. We previously reported the effectiveness of a combined continuous density/osmolality gradient for the supplemental purification of human islets. We herein applied a combined continuous density/osmolality gradient for regular purification. The islets were purified with a continuous density gradient without osmolality preparation [continuous density/normal osmolality (CD/NO)] or continuous density/osmolality solution with osmolality preparation by 10× Hank's balanced salt solution (HBSS) [continuous density/continuous osmolality (CD/CO)]...
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28174674/the-evaluation-of-islet-purification-methods-that-use-large-bottles-to-create-a-continuous-density-gradient
#2
Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi
Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. The most common method of islet purification is density gradient centrifugation using a COBE 2991 cell processor. However, this method can damage islets mechanically through its high shearing force. We recently reported that a new purification method using large plastic bottles effectively achieves a high yield of islets from the porcine pancreas. In the present study, we evaluated the methods of making a continuous density gradient...
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28174673/enhanced-adipogenic-differentiation-of-human-adipose-derived-stem-cells-in-an-in-vitro-microenvironment-the-preparation-of-adipose-like-microtissues-using-a-three-dimensional-culture
#3
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi
The application of stem cells for cell therapy has been extensively studied in recent years. Among the various types of stem cells, human adipose tissue-derived stem cells (ASCs) can be obtained in large quantities with relatively few passages, and they possess a stable quality. ASCs can differentiate into a number of cell types, such as adipose cells and ectodermal cells. We therefore focused on the in vitro microenvironment required for such differentiation and attempted to induce the differentiation of human stem cells into microtissues using a microelectromechanical system...
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28174672/immunomodulatory-effects-of-adipose-tissue-derived-stem-cells-on-concanavalin-a-induced-acute-liver-injury-in-mice
#4
Yasuma Yoshizumi, Hiroshi Yukawa, Ryoji Iwaki, Sanae Fujinaka, Ayano Kanou, Yuki Kanou, Tatsuya Yamada, Shingo Nakagawa, Tomomi Ohara, Kenta Nakagiri, Yusuke Ogihara, Yoko Tsutsui, Yumi Hayashi, Masatoshi Ishigami, Yoshinobu Baba, Tetsuya Ishikawa
Cell therapy with adipose tissue-derived stem cells (ASCs) is expected to be a candidate for the treatment of fulminant hepatic failure (FHF), which is caused by excessive immune responses. In order to evaluate the therapeutic effects of ASCs on FHF, the in vitro and in vivo immunomodulatory effects of ASCs were examined in detail in the mouse model. The in vitro effects of ASCs were examined by assessing their influence on the proliferation of lymphomononuclear cells (LMCs) stimulated with three kinds of mitogens: phorbol 12-myristate 13-acetate (PMA) plus ionomycin, concanavalin A (ConA), and lipopolysaccharide (LPS)...
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28174671/evaluation-of-serum-free-xeno-free-cryopreservation-solutions-for-human-adipose-derived-mesenchymal-stem-cells
#5
Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi
Adipose-derived mesenchymal stem cells (ASCs) have the potential to differentiate into cells of mesodermal origin, such as osteoblasts, adipocytes, myocytes, and chondrocytes, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. For clinical application of ASCs, serum-free, xeno-free cryopreservation solutions should be used. This study determined the viability and adipo-osteogenic potential of cryopreserved ASCs using four cryopreservation solutions: 10% DMSO, Cell Banker 2 (serum free), Stem Cell Banker (=Cell Banker 3: serum free, xeno free), and TC protector (serum free, xeno free)...
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28174670/challenges-for-production-of-human-transplantable-organ-grafts
#6
REVIEW
Eiji Kobayashi
The described research methods explain how you could generate a three-dimensional kidney, based on recent research results. The first method is to fabricate human organs in a pig body. The second is to transplant the so-called "organ bud" into a patient's body for further development. The third method is to regenerate organs by filling cells into the cytoskeleton as a scaffold. Research for the in vitro fabrication of organ buds has been elaborately accelerated. The organ bud transplantation has been confronted with issues of continuity with the original organs, so the development of technology for achieving continuity between a transplanted organ bud and the existing organs is progressing well...
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28174669/cell-therapy-for-liver-disease-using-bioimaging-rats
#7
REVIEW
Junko Haga, Shin Enosawa, Eiji Kobayashi
Advances in stem cell research suggest that cell therapy is a potential alternative to liver transplantation. The use of individualized and minimally invasive cell therapy is desirable to avoid rejection and reduce patient burden. While allo-hepatocyte transplantation has been performed for metabolic hepatic disease, auto-bone marrow transplantation (BMT) has shifted toward mesenchymal stem cells (MSCs) transplantation for liver cirrhosis. In this article, an overview of cell transplantation research for liver disease is provided through our recent rat studies...
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28174668/facilitating-transplantation
#8
Hirofumi Noguchi
No abstract text is available yet for this article.
January 8, 2017: Cell Medicine
https://www.readbyqxmd.com/read/28003935/aggregation-of-engineered-human-%C3%AE-cells-into-pseudoislets-insulin-secretion-and-gene-expression-profile-in-normoxic-and-hypoxic-milieu
#9
Marie-José Lecomte, Séverine Pechberty, Cécile Machado, Sandra Da Barroca, Philippe Ravassard, Raphaël Scharfmann, Paul Czernichow, Bertrand Duvillié
Innovative treatments to cure type 1 diabetes are being actively researched. Among the different strategies, the replacement of β-cells has given promising results. Classically, islets from cadaveric donors are transplanted into diabetic patients, but recently phase I clinical trials that use stem cell-derived β-cells have been started. Such protocols require either an immunosuppressive treatment or the macroencapsulation of the β-cells. They involve cell aggregation and the exposure of the cells to hypoxia...
December 3, 2016: Cell Medicine
https://www.readbyqxmd.com/read/28003934/stability-enhancement-using-hyaluronic-acid-gels-for-delivery-of-human-fetal-progenitor-tenocytes
#10
A Grognuz, C Scaletta, A Farron, D P Pioletti, W Raffoul, L A Applegate
Tendon afflictions are very common, and their negative impact is high both at the workplace and in leisure activities. Tendinopathies are increasing in prevalence and can lead to tendon ruptures, where healing is a long process with outcomes that are often disappointing. Human fetal progenitor tenocytes (hFPTs) have been recently tested in vitro as a potential cell source to stimulate tendon regeneration. The aim of the present study was to compare different commercial hyaluronic acid (HA) gels, which could be used to resuspend hFPTs in a formulation that would allow for good delivery of the cells...
December 3, 2016: Cell Medicine
https://www.readbyqxmd.com/read/28003933/%C3%AE-sma-expression-in-large-colonies-of-colony-forming-units-fibroblast-as-an-early-predictor-of-bone-marrow-msc-expandability
#11
Irina Aizman, William S Holland, Cher Yang, Damien Bates
Clinical applications of mesenchymal stromal cells (MSCs) require the manufacture of large cell lots, which involves multiple passages for cell expansion and sometimes genetic modification. MSCs from various sources, including bone marrow (BM), exhibit high donor-to-donor variability in their growth characteristics. This can lead to unpredictable manufacturing outcomes with respect to success or failure of individual lots. Early determination of lot success has the potential to reduce the cost and improve the efficiency of the MSC manufacturing process...
December 3, 2016: Cell Medicine
https://www.readbyqxmd.com/read/28003932/allogeneic-mesenchymal-stem-cell-transplantation-in-dogs-with-keratoconjunctivitis-sicca
#12
Maura K W Bittencourt, Michele A Barros, João Flávio P Martins, Jose Paulo C Vasconcellos, Bruna P Morais, Celine Pompeia, Matheus Domingues Bittencourt, Karine Dos Santos Evangelho, Irina Kerkis, Cristiane V Wenceslau
Keratoconjunctivitis sicca (KCS) is a dysfunction in tear production associated with clinical signs, which include conjunctival hyperemia, ocular discharge, discomfort, pain, and, eventually, corneal vascularization and pigmentation. Immunosuppressive drugs are routinely administrated for long periods to treat KCS but with side effects and limited results. Evaluation of the clinical benefits of intralacrimal transplantation of allogeneic mesenchymal stem cells (MSCs) in dogs with mild-moderate and severe KCS was done...
December 3, 2016: Cell Medicine
https://www.readbyqxmd.com/read/26858909/fluorescence-quenching-of-cdse-zns-quantum-dots-by-using-black-hole-quencher-molecules-intermediated-with-peptide-for-biosensing-application
#13
Sreenadh Sasidharan Pillai, Hiroshi Yukawa, Daisuke Onoshima, Vasudevanpillai Biju, Yoshinobu Baba
Quantum dots (QDs) have recently been investigated as fluorescent probes for detecting a very small number of biomolecules and live cells; however, the establishment of molecular imaging technology with on-off control of QD fluorescence remains to be established. Here we have achieved the fluorescence off state of QDs with the conjugation of black hole quencher (BHQ) molecules intermediated with peptide by using streptavidin-QDs585 and biotin-pep-BHQ-1. The fluorescence of streptavidin-QDs585 was decreased by the addition of biotin-pep-BHQ-1 in a dose-dependent manner...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858908/spheroid-formation-and-evaluation-of-hepatic-cells-in-a-three-dimensional-culture-device
#14
Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, Tohru Yagi, Shuji Hayashi
In drug discovery, it is very important to evaluate liver cells within an organism. Compared to 2D culture methods, the development of 3D culture techniques for liver cells has been successful in maintaining long-term liver functionality with the formation of a hepatic-specific structure. The key to performing drug testing is the establishment of a stable in vitro evaluation system. In this article, we report a Tapered Stencil for Cluster Culture (TASCL) device developed to create liver spheroids in vitro. The TASCL device will be applied as a toxicity evaluation system for drug discovery...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858907/human-laminin-isotype-coating-for-creating-islet-cell-sheets
#15
Shingo Yamashita, Kazuo Ohashi, Rie Utoh, Teruo Okano, Masakazu Yamamoto
Our experimental approach toward the development of new islet-based treatment for diabetes mellitus has been the creation of a monolayered islet cell construct (islet cell sheet), followed by its transplantation into a subcutaneous pocket. Previous studies describe rat laminin-5 (chain composition: α3, β3, γ2) as a suitable extracellular matrix (ECM) for surfaces comprised of a coated temperature-responsive polymer, poly(N-isopropylacrylamide) (PIPAAm). To progress toward the clinical application of this approach, the present study attempted to identify an optimal human ECM as a coating material on PIPAAm surfaces, which allowed islet cells to attach on the surfaces and subsequently to be harvested as a monolithic cell sheet...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858906/efficient-gene-transduction-of-dispersed-islet-cells-in-culture-using-fiber-modified-adenoviral-vectors
#16
Hiroyuki Hanayama, Kazuo Ohashi, Rie Utoh, Hirofumi Shimizu, Kazuya Ise, Fuminori Sakurai, Hiroyuki Mizuguchi, Hiroyuki Tsuchiya, Teruo Okano, Mitsukazu Gotoh
To establish novel islet-based therapies, our group has recently developed technologies for creating functional neo-islet tissues in the subcutaneous space by transplanting monolithic sheets of dispersed islet cells (islet cell sheets). Improving cellular function and viability are the next important challenges for enhancing the therapeutic effects. This article describes the adenoviral vector-mediated gene transduction of dispersed islet cells under culture conditions. Purified pancreatic islets were obtained from Lewis rats and dissociated into single islet cells...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858905/islet-culture-preservation-before-islet-transplantation
#17
REVIEW
Hirofumi Noguchi, Chika Miyagi-Shiohira, Kiyoto Kurima, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita
Although islet culture prior to transplantation provides flexibility for the evaluation of isolated islets and the pretreatment of patients, it is well known that isolated islets deteriorate rapidly in culture. Human serum albumin (HSA) is used for medium supplementation instead of fetal bovine serum (FBS), which is typically used for islet culture research, to avoid the introduction of xenogeneic materials. However, FBS contains several factors that are beneficial to islet viability and which also neutralize the endogenous pancreatic enzymes or exogenous enzymes left over from the isolation process...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858904/choice-of-feeders-is-important-when-first-establishing-ipscs-derived-from-primarily-cultured-human-deciduous-tooth-dental-pulp-cells
#18
Issei Saitoh, Emi Inada, Yoko Iwase, Hirofumi Noguchi, Tomoya Murakami, Miki Soda, Naoko Kubota, Hiroko Hasegawa, Eri Akasaka, Yuko Matsumoto, Kyoko Oka, Youichi Yamasaki, Haruaki Hayasaki, Masahiro Sato
Feeder cells are generally required to maintain embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs). Mouse embryonic fibroblasts (MEFs) isolated from fetuses and STO mouse stromal cell line are the most widely used feeder cells. The aim of this study was to determine which cells are suitable for establishing iPSCs from human deciduous tooth dental pulp cells (HDDPCs). Primary cultures of HDDPCs were cotransfected with three plasmids containing human OCT3/4, SOX2/KLF4, or LMYC/LIN28 and pmaxGFP by using a novel electroporation method, and then cultured in an ESC qualified medium for 15 days...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858903/cryopreservation-of-adipose-derived-mesenchymal-stem-cells
#19
REVIEW
Chika Miyagi-Shiohira, Kiyoto Kurima, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, Hirofumi Noguchi
Mesenchymal stem cells (MSCs) have the potential to differentiate into cells of mesodermal origin such as osteoblasts, adipocytes, myocytes, and chondrocytes. They possess an immunosuppressive effect, which makes them a viable cell population for the cell-based therapy of treatment-resistant immune diseases. Adipose-derived mesenchymal stem cells (ASCs) have been demonstrated to have the ability to acquire the properties of subcutaneous adipose tissue particularly easily, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells...
December 17, 2015: Cell Medicine
https://www.readbyqxmd.com/read/26858902/creating-a-future-of-transplantation
#20
Hirofumi Noguchi
No abstract text is available yet for this article.
December 17, 2015: Cell Medicine
journal
journal
43308
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"