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Analytical Cellular Pathology (Amsterdam)

Antonio Ieni, Valeria Barresi, Giovanni Branca, Rosario Alberto Caruso, Giovanni Tuccari
Aim. The role of mast cells in cell-cell immune interactions has received increasing attention, although their functional interaction with neutrophils still remains to be clarified in tumors. The aim of the present study was to investigate the association between mast cells and neutrophils in a series of gastric carcinomas (GC). Patients and Methods. 52 surgically resected GC specimens were routinely processed for both light and electron microscopy. Only cases showing both mast cells and neutrophils in the tumor stroma were considered in the analysis...
2016: Analytical Cellular Pathology (Amsterdam)
Rogelio González-González, Nelly Molina-Frechero, Ramón G Carreón-Burciaga, Sandra López-Verdín, Carlos Robles-Bonilla, Vanesa Pereira-Prado, Ronell Bologna-Molina
Ameloblastoma is a common and unpredictable odontogenic tumor with high relapse rates. Several studies assessing the proliferative capacity of these neoplasms have been published, mainly using the protein Ki-67. Cell counts must be completed to determine the cell proliferation rate. Multiple methods have been developed for this purpose. The most widely used method is the labeling index, which has undergone changes over time to better facilitate cell counting. Here, we compared manual cell counting methods with automated cell counting (ImmunoRatio) to determine the relative effectiveness of these methods...
2016: Analytical Cellular Pathology (Amsterdam)
Răzvan Ciortea, Costin Berceanu, Andrei Mihai Măluţan, Radu Mocan, Cristian Iuhas, Carmen Elena Bucuri, Maria Patricia Rada, Dan Mihu
Introduction. The identification of biological markers that indicate an increased risk for the development or recurrence of endometrial cancer (EC) in obese women might be useful for decreasing EC mortality and morbidity. Glucose-regulated protein 78 (GRP78) is a major protein of the endoplasmic reticulum expressed in all normal cells. Overexpression of GRP78 has been reported to be a tumoral biomarker. Increased detection of GRP78 is positively correlated with the tumoral stage and prognosis. This study aimed to identify a correlation between intraperitoneal fat, plasma GRP78 levels, and EC...
2016: Analytical Cellular Pathology (Amsterdam)
Ioana Cristina Rotar, Diana Elena Dumitras, Radu Anghel Popp, Felicia Maria Petrisor, Paul Cotutiu, Florin Stamatian, Daniel Muresan
Aim. The present study aims to analyze the potential role of VEGF +936 C/T polymorphism in cervical intraepithelial neoplasia. Material and Method. One hundred and eighty-six patients were included in the study: 75 cases (patients diagnosed with CIN) and 111 controls (negative for both HPV testing and cytology). For each patient a single visit was scheduled when colposcopy was performed. From cervical specimen, cytology and HPV testing were performed and from peripheral blood VEGF +936 genotyping was determined...
2016: Analytical Cellular Pathology (Amsterdam)
Olga V Kovaleva, Daria V Samoilova, Maria S Shitova, Alexei Gratchev
Tumor associated macrophages (TAMs) are an important element of tumor stroma. They originate from blood monocytes attracted by chemokines and cytokines produced by tumor cells and, being instructed by tumor microenvironment, develop into potent tumor-supporting cell population. TAMs were demonstrated to directly stimulate tumor cell proliferation and to promote angiogenesis. Further TAMs provide for efficient immune escape by producing immunosuppressive cytokines and facilitate tumor dissemination by producing extracellular matrix remodeling enzymes...
2016: Analytical Cellular Pathology (Amsterdam)
Spoorthi Ravi Banavar, Prashanthi Chippagiri, Rohit Pandurangappa, Saileela Annavajjula, Premalatha Bidadi Rajashekaraiah
Background. Microscopes are omnipresent throughout the field of biological research. With microscopes one can see in detail what is going on at the cellular level in tissues. Though it is a ubiquitous tool, the limitation is that with high magnification there is a small field of view. It is often advantageous to see an entire sample at high magnification. Over the years technological advancements in optics have helped to provide solutions to this limitation of microscopes by creating the so-called dedicated "slide scanners" which can provide a "whole slide digital image...
2016: Analytical Cellular Pathology (Amsterdam)
Chengzhong Cai, Dmitry A Nedosekin, Yulian A Menyaev, Mustafa Sarimollaoglu, Mikhail A Proskurnin, Vladimir P Zharov
Control of sickle cell disease (SCD) stage and treatment efficiency are still time-consuming which makes well-timed prevention of SCD crisis difficult. We show here that in vivo photoacoustic (PA) flow cytometry (PAFC) has a potential for real-time monitoring of circulating sickled cells in mouse model. In vivo data were verified by in vitro PAFC and photothermal (PT) and PA spectral imaging of sickle red blood cells (sRBCs) expressing SCD-associated hemoglobin (HbS) compared to normal red blood cells (nRBCs)...
2016: Analytical Cellular Pathology (Amsterdam)
A J I S Rathnayake, H W W Goonasekera, V H W Dissanayake
Bone marrow (BM) mesenchymal stem/stromal cells (MSCs) are vital in hematopoiesis. Whether BM-MSCs alter their characteristics in Myelodysplastic Syndromes (MDS) is still controversial. We characterized MSCs of de novo MDS patients in Sri Lanka who have not been reported previously in the literature. We also analyzed MSCs derived from different MDS subtypes. MSCs were culture-expanded, characterized by flow cytometry, and induced towards osteogenic and adipogenic differentiation. Growth properties were determined using growth curves and population doubling times...
2016: Analytical Cellular Pathology (Amsterdam)
Katrin Kreienbring, Annika Franz, Rolf Richter, Duska Dragun, Harald Heidecke, Ralf Dechend, Dominik N Muller, Jalid Sehouli, Elena I Braicu
Aim. The purpose of the present study was to analyze the predictive and prognostic role of soluble (pro)renin receptor (sPRR) as a biomarker for clinicopathological outcome in patients with primary epithelial ovarian cancer (EOC). As part of the renin-angiotensin system (RAS) whose activity is known to increase in ovarian cancer patients, the relation of sPRR and ovarian cancer should be further investigated. Patients and Methods. In this study 197 patients with primary EOC in our institution from 2000 to 2011 were included...
2016: Analytical Cellular Pathology (Amsterdam)
Baixin Ye, Qingping Gao, Zhi Zeng, Creed M Stary, Zhihong Jian, Xiaoxing Xiong, Lijuan Gu
Cellular heterogeneity is a fundamental characteristic of many cancers. A lack of cellular homogeneity contributes to difficulty in designing targeted oncological therapies. Therefore, the development of novel methods to determine and characterize oncologic cellular heterogeneity is a critical next step in the development of novel cancer therapies. Single-cell sequencing (SCS) technology has been recently employed for analyzing the genetic polymorphisms of individual cells at the genome-wide level. SCS requires (1) precise isolation of the single cell of interest; (2) isolation and amplification of genetic material; and (3) descriptive analysis of genomic, transcriptomic, and epigenomic data...
2016: Analytical Cellular Pathology (Amsterdam)
Jie Su, Xuan Xu, Yongjun He, Jinming Song
We proposed a method for automatic detection of cervical cancer cells in images captured from thin liquid based cytology slides. We selected 20,000 cells in images derived from 120 different thin liquid based cytology slides, which include 5000 epithelial cells (normal 2500, abnormal 2500), lymphoid cells, neutrophils, and junk cells. We first proposed 28 features, including 20 morphologic features and 8 texture features, based on the characteristics of each cell type. We then used a two-level cascade integration system of two classifiers to classify the cervical cells into normal and abnormal epithelial cells...
2016: Analytical Cellular Pathology (Amsterdam)
Erol Aktunc, Zehra Safi Oz, Sibel Bektas, Cevdet Altinyazar, Rafet Koca, Serdar Bostan
BACKGROUND: The aim of this study was to compare the cytomorphometric characteristics of the buccal cells of Behçet's disease patients with those of healthy controls. METHODS: This case-control study compared a group of 30 patients with Behçet's disease with an age- and gender-matched control group of 30 healthy individuals. The buccal mucosal smears were stained using the Papanicolaou technique for cytomorphometric analyses. The nuclear and cytoplasmic areas were evaluated using digital image analysis; the ratio of nuclear to cytoplasmic areas and nuclear roundness are presented...
2016: Analytical Cellular Pathology (Amsterdam)
Rongwei Zhai, Na Zheng, Joshua Rizak, Xintian Hu
Many studies have reported that methanol toxicity to primates is mainly associated with its metabolites, formaldehyde (FA) and formic acid. While methanol metabolism and toxicology have been best studied in peripheral organs, little study has focused on the brain and no study has reported experimental evidence that demonstrates transformation of methanol into FA in the primate brain. In this study, three rhesus macaques were given a single intracerebroventricular injection of methanol to investigate whether a metabolic process of methanol to FA occurs in nonhuman primate brain...
2016: Analytical Cellular Pathology (Amsterdam)
Arif Malik, Misbah Sultana, Aamer Qazi, Mahmood Husain Qazi, Gulshan Parveen, Sulayman Waquar, Abdul Basit Ashraf, Mahmood Rasool
Cancer originates from genetic mutations accumulation. Cancer stem cells have been depicted as tumorigenic cells that can differentiate and self-renew. Cancer stem cells are thought to be resistant to conventional therapy like chemotherapy and radiation therapy. Radiation therapy and chemotherapy damage carcinomic DNA cells. Because of the ability of cancer stem cells to self-renew and reproduce malignant tumors, they are the subject of intensive research. In this review, CSCs radioresistant mechanisms which include DNA damage response and natural radiosensitizers have been summed up...
2016: Analytical Cellular Pathology (Amsterdam)
Mei Lin, Junxing Huang, Dongsheng Zhang, Xingmao Jiang, Jia Zhang, Hong Yu, Yanhong Xiao, Yujuan Shi, Ting Guo
An effective strategy has been developed for synthesis of radionuclide immune albumin nanospheres ((131)I-antiAFPMcAb-GCV-BSA-NPs). In vitro as well as in vivo targeting of (131)I-antiAFPMcAb-GCV-BSA-NPs to AFP-positive hepatoma was examined. In cultured HepG2 cells, the uptake and retention rates of (131)I-antiAFPMcAb-GCV-BSA-NPs were remarkably higher than those of (131)I alone. As well, the uptake rate and retention ratios of (131)I-antiAFPMcAb-GCV-BSA-NPs in AFP-positive HepG2 cells were also significantly higher than those in AFP-negative HEK293 cells...
2016: Analytical Cellular Pathology (Amsterdam)
Rene Franzen, Nasrin Kianimanesh, Rudolf Marx, Asma Ahmed, Norbert Gutknecht
Dental tooth restorative procedures may weaken the structural integrity of the tooth, with the possibility of leading to fracture. In this study we present findings of coronal dentin strength after different techniques of surface modification. The fracture strength of dentin beams after superficial material removal with a fine diamond bur high speed drill hand piece, Er:YAG (2.94 μm, 8 J/cm(2)), and Er,Cr:YSGG (2.78 μm, 7.8 J/cm(2)) laser irradiation slightly above the ablation threshold was measured by a four-point bending apparatus...
2016: Analytical Cellular Pathology (Amsterdam)
Yicheng Chen, Yueping Wang, Yanlan Yu, Liwei Xu, Youyun Zhang, Shicheng Yu, Gonghui Li, Zhigeng Zhang
Radiotherapy for prostate cancer has been gradually carried out in recent years; however, acquired radioresistance often occurred in some patients after radiotherapy. HBP1 (HMG-box transcription factor 1) is a transcriptional inhibitor which could inhibit the expression of dozens of oncogenes. In our previous study, we showed that the expression level of HBP1 was closely related to prostate cancer metastasis and prognosis, but the relationship between HBP1 and radioresistance for prostate cancer is largely unknown...
2016: Analytical Cellular Pathology (Amsterdam)
Chi-Hsuan Tsou, Yi-Chien Lu, Ang Yuan, Yeun-Chung Chang, Chung-Ming Chen
The blood vessel density in a cancerous tissue sample may represent increased levels of tumor growth. However, identifying blood vessels in the histological (tissue) image is difficult and time-consuming and depends heavily on the observer's experience. To overcome this drawback, computer-aided image analysis frameworks have been investigated in order to boost object identification in histological images. We present a novel algorithm to automatically abstract the salient regions in blood vessel images. Experimental results show that the proposed framework is capable of deriving vessel boundaries that are comparable to those demarcated manually, even for vessel regions with weak contrast between the object boundaries and background clutter...
2015: Analytical Cellular Pathology (Amsterdam)
Mei Lin, Junxing Huang, Yujuan Shi, Yanhong Xiao, Ting Guo
Radiation-gene therapy, a dual anticancer strategy of radiation therapy and gene therapy through connecting radiation-inducible regulatory sequence to therapeutic gene, leading to the gene being induced to express by radiation while radiotherapy is performed and finally resulting in a double synergistic antitumor effect of radiation and gene, has become one of hotspots in the field of cancer treatment in recent years. But under routine dose of radiation, especially in the hypoxia environment of solid tumor, it is difficult for this therapy to achieve desired effect because of low activity of radiation-inducible regulatory elements, low level and transient expression of target gene induced by radiation, inferior target specificity and poor biosecurity, and so on...
2015: Analytical Cellular Pathology (Amsterdam)
Daniela Cabibi, Fabrizio Bronte, Rossana Porcasi, Sabrina Ingrao, Antonino Giulio Giannone, Marcello Maida, Maria Grazia Bavetta, Salvatore Petta, Vito Di Marco, Vincenza Calvaruso
Background and Aim. The best staining to evaluate liver fibrosis in liver hepatitis is still a debated topic. This study aimed to compare Masson's trichrome (MT), Sirius Red (SR), and orcein stainings in evaluating liver fibrosis in chronic HCV hepatitis (CHC) with semiquantitative and quantitative methods (Collagen Proportionate Area (CPA) by Digital Image Analysis (DIA)) and correlate them with transient elastography (TE). Methods. Liver stiffness evaluation of 111 consecutive patients with CHC was performed by TE...
2015: Analytical Cellular Pathology (Amsterdam)
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