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Analytical Cellular Pathology (Amsterdam)

Bo Wu, Haihua Yang, Shenpeng Ying, Hongsheng Lu, Wei Wang, Jiaming Lv, Huacai Xiong, Wei Hu
Background and Aims: In patients with nasopharyngeal carcinoma (NPC), local treatment failure and distant metastasis contribute largely to poor outcomes. The nasopharynx is an important lymphoid tissue, and NPC tumourigenesis and development are partly attributed to immune system disorders. Human leukocyte antigen F (HLA-F) has shown a close correlation with NPC in many genome-wide association studies (GWASs). However, clinical studies rarely explore the relationship of HLA-F expression with the clinical parameters and outcomes in patients with NPC...
2018: Analytical Cellular Pathology (Amsterdam)
Vladimir Jurišić, Jasmina Obradovic, Sonja Pavlović, Nataša Djordjevic
Recently, epidermal growth factor receptor (EGFR) was a key molecule in investigation of lung cancer, and it was a target for a new therapeutic strategy, based on molecular analyses. In this review, we have summarized some issues considering the role of EGFR in lung cancer, its coding gene, and its promoter gene polymorphisms (SNPs) -216G/T and -191C/A in non-small-cell lung cancer (NSCLC). The position of the SNPs indicates their significant role in EGFR regulation. The accumulation of knowledge regarding SNPs lately suggests their significant and important role in the onset of carcinogenesis, the prediction of the onset of metastases, the response to therapy with TKI inhibitors, and the onset of toxic effects of the applied therapy...
2018: Analytical Cellular Pathology (Amsterdam)
Alexander Glassmann, Carmen Carrillo Garcia, Viktor Janzen, Dominik Kraus, Nadine Veit, Jochen Winter, Rainer Probstmeier
Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis. Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state...
2018: Analytical Cellular Pathology (Amsterdam)
Sebastian Marciniak, Artur Wnorowski, Katarzyna Smolińska, Beata Walczyna, Waldemar Turski, Tomasz Kocki, Piotr Paluszkiewicz, Jolanta Parada-Turska
Acute liver failure (ALF) is a life-threatening disorder of liver function. Kynurenic acid (KYNA), a tryptophan metabolite formed along the kynurenine metabolic pathway, possesses anti-inflammatory and antioxidant properties. Its presence in food and its potential role in the digestive system was recently reported. The aim of this study was to define the effect of KYNA on liver failure. The Wistar rat model of thioacetamide-induced liver injury was used. Morphological and biochemical analyses as well as the measurement of KYNA content in liver and hepatoprotective herbal remedies were conducted...
2018: Analytical Cellular Pathology (Amsterdam)
Diyou Li, Yinglin Pan, Yating Huang, Ping Zhang, Xuhong Fang
Ovarian cancer is the most lethal gynecologic cancer and currently ranks fifth in causing cancer-related deaths among women. P21cdc42/rac1 -activated kinase 5 (PAK5) is a newly identified protein that has been indicated to have oncogenic potential. The present study investigated the expression level of PAK5 in clinical ovarian cancer and the functional roles of PAK5 in ovarian cancer progression. It was initially found that PAK5 was highly expressed in ovarian cancer tissues, particularly in patients with distant metastasis...
2018: Analytical Cellular Pathology (Amsterdam)
Sonja S Mojsilovic, Slavko Mojsilovic, Suncica Bjelica, Juan F Santibanez
Transforming growth factor-beta (TGF- β ) has been demonstrated as a key regulator of immune responses including monocyte/macrophage functions. TGF- β regulates macrophage cell migration and polarization, as well as it is shown to modulate macrophage urokinase-type plasminogen activator (uPA) production, which also contributes to macrophage chemotaxis and migration toward damaged or inflamed tissues. Microtubule (MT) cytoskeleton dynamic plays a key role during the cell motility, and any interference on the MT network profoundly affects cell migration...
2018: Analytical Cellular Pathology (Amsterdam)
Bushra Nissar, Showkat A Kadla, Nuzhat Shaheen Khan, Idrees A Shah, Misbah Majid, Falaque Ul Afshan, Bashir Ahmad Ganai
Coding polymorphisms in several DNA repair genes have been reported to affect the DNA repair capacity and are associated with genetic susceptibility to many human cancers, including gastric cancer. An understanding of these DNA repair gene polymorphisms might assess not only the risk of humans exposed to environmental carcinogens but also their responses to different therapeutical approaches, which target the DNA repair pathway. In the present study, polymorphic variants of two DNA repair genes, XRCC1 Arg399Gln and XPD Lys751Gln, were chosen to be studied in association with gastric cancer susceptibility in the Kashmiri population...
2018: Analytical Cellular Pathology (Amsterdam)
Emina Babarović, Ivan Franin, Marko Klarić, Ani Mihaljević Ferrari, Ružica Karnjuš-Begonja, Senija Eminović, Damjana Verša Ostojić, Danijela Vrdoljak-Mozetič
Objective: Adult granulosa cell tumors (AGCTs) represent 2%-5% of all ovarian malignancies. The aim of this study was to analyze clinical and pathohistological parameters and their impact on recurrence, overall, and disease-free survival in FIGO stage I AGCT patients. Methods: The tumor specimens analyzed in this retrospective study were obtained from a total of 36 patients with diagnosis of ovarian AGCT surgically treated at the Department of Gynecology, Rijeka University Hospital Centre, between 1994 and 2012...
2018: Analytical Cellular Pathology (Amsterdam)
Sellamuthu Subbanna Gounder, Basri Johan Jeet Abdullah, Nur Ezzati Izyan Binti Mohd Radzuanb, Farah Dalila Binti Mohd Zain, Nurhidayah Bt Mohamad Sait, Corine Chua, Baskar Subramani
Age-associated changes in natural killer (NK) cell population, phenotype, and functions are directly attributed to the risk of several diseases and infections. It is predicted to be the major cause of the increase in mortality. Based on the surface density of CD56, NK cells are subdivided into two types, such as CD56bright and CD56dim cells, which represent cytokine production and cytotoxicity. In our study, we have examined the age-associated changes in the NK cell population and their subsets at different age groups of males and females (at a range from 41 to 80 years)...
2018: Analytical Cellular Pathology (Amsterdam)
Jenefa L Canoy, Jayzon G Bitacura
Brown macroalgae species are constantly reported as potential sources of bioactive compounds useful in inhibiting cell proliferation and vascular formation. Thus, this study was conducted to determine and compare the in vitro cytotoxic activities of Turbinaria ornata Agardh and Padina australis Hauck ethanolic extracts against baker's yeast ( Saccharomyces cerevisiae ) using the resazurin reduction test (RRT) and investigate their in vivo antiangiogenic activity through duck ( Anas platyrhynchos ) chorioallantoic membrane (CAM) assay...
2018: Analytical Cellular Pathology (Amsterdam)
Tsung-Jung Tsai, Yun-Ping Lim, Wen-Ying Chao, Chien-Chin Chen, Yi-Ju Chen, Ching-Yen Lin, Ying-Ray Lee
Objective: Human colorectal cancer (CRC) is the third most common cancer; patients with metastatic colorectal cancer (mCRC) show poor prognosis than those with CRC cases. There are no reliable molecular biomarkers for the diagnosis of CRC prognosis except with pathological features. Therefore, it is urgent to develop a biomarker for diagnosis and/or prediction of human CRC. In addition, capping actin protein (CapG) belongs to the gelsolin family and has been reported to contribute on tumor invasion/metastasis in multiple human cancers...
2018: Analytical Cellular Pathology (Amsterdam)
Ivana Miše, Majda Vučić
Syndecan-1 (Sdc1) is a transmembrane heparan sulfate proteoglycan, an extracellular matrix receptor involved in intercellular communication, proliferation, angiogenesis, and metastasis. This study determined and compared Sdc1 expression in the tumor cells and stroma of 30 invasive lobular and 30 invasive ductal breast carcinomas (ILCs/IDCs), also in the axillary node metastases of ductal type, and correlated it with clinical and tumor parameters. Sdc1 was expressed in the epithelium of 90% carcinoma of both histological types...
2018: Analytical Cellular Pathology (Amsterdam)
Ying Chen, Qianjun Chen, Fengfeng Xie, Hui Peng, Yanlan Wu, Shaowen Zhong, Zhiyu Wang, Wenxia Li, Wanjun Xie
Aims: The aim of the present study is to investigate whether the aqueous extract from Huaier, a traditional Chinese medicine (TCM), can affect the expression of Duffy antigen receptor for chemokines (DARC) and its ligands. Moreover, we compare the status of DARC in primary and metastatic breast cancer tissues from the same patient. Methods: Immunohistochemistry was used to detect the expression of DARC in primary and metastatic focuses in 30 patients with breast cancer...
2018: Analytical Cellular Pathology (Amsterdam)
Yi Ning Xie, Lian Yu, Guo Hui Guan, Yong Jun He
DNA ploidy analysis of cells is an automation technique applied in pathological diagnosis. It is important for this technique to classify various nuclei images accurately. However, the lack of overlapping nuclei images in training data (imbalanced training data) results in low recognition rates of overlapping nuclei images. To solve this problem, a new method which synthesizes overlapping nuclei images with single-nuclei images is proposed. Firstly, sample selection is employed to make the synthesized samples representative...
2018: Analytical Cellular Pathology (Amsterdam)
Svetla Nikolova, Milka Dikova, Dobrin Dikov, Assen Djerov, Alexey Savov, Ivo Kremensky, Alexandre Loukanov
Idiopathic scoliosis (IS) is a common medical condition beginning in childhood and characterized by strong evidence for a genetic susceptibility to three-dimensional spinal deformity. The primary goal of the current case-control study is to examine the association between the TGFB1 (-509C/T) functional polymorphic variant and genetic predisposition to IS in the Bulgarian population and the genotype-phenotype correlations in distinct case-control subgroups based on age at onset, family history, and gender. A total of 127 patients with primary scoliosis and 254 gender-matched control subjects were recruited...
2018: Analytical Cellular Pathology (Amsterdam)
Ping Jin, Yi Song, Guiyuan Yu
Objective: In the current study, the role of abnormal methylation of Wnt5a gene promoter regions in human epithelial ovarian cancer was investigated. Methods: Wnt5a expressions were examined by immunohistochemistry in epithelial ovarian tissues (30 normal and 79 human EOC tissues). SKOV3 cells were treated with different concentrations of 5-Aza-CdR (0.5, 5, and 50  μ mol/L). The methylation status of the Wnt5a promoter was analyzed using a methylation-specific polymerase chain reaction (MSP), and the expression level of Wnt5a mRNA was detected using quantitative real-time polymerase chain reaction (qRT-PCR)...
2018: Analytical Cellular Pathology (Amsterdam)
Neeti Swarup, Meghanand T Nayak, Zoya Chowdhary, Monica Mehendiratta, Shikha Khatana, Su Jung Choi, Chandarani Sagolsem
Growth factors like bone morphogenetic protein 4 (BMP4) and fibroblast growth factor 8 (FGF8) play a major role in organogenesis and specifically in odontogenesis. They are also believed to have a role in oncogenesis. Thus, any discrepancies in their standard behavior and activity would lead to serious abnormalities including odontogenic cyst and tumors. The present research work investigated the expression of BMP4 and FGF8 in odontogenic tumors (OT) and cyst as well as developing tooth germs to elucidate their roles...
2018: Analytical Cellular Pathology (Amsterdam)
Anubhav Chakraborty, Pragasam Viswanathan
Over the years, the epigenetic landscape has grown increasingly complex. Until recently, methylation of DNA and histones was considered one of the most important epigenetic modifications. However, with the discovery of enzymes involved in the demethylation process, several exciting prospects have emerged that focus on the dynamic regulation of methylation and its crucial role in development and disease. An interplay of the methylation-demethylation machinery controls the process of gene expression. Since acute kidney injury (AKI), a major risk factor for chronic kidney disease and death, is characterised by aberrant expression of genes, understanding the dynamics of methylation and demethylation will provide new insights into the intricacies of the disease...
2018: Analytical Cellular Pathology (Amsterdam)
Ran Gao, Rui Zhang, Cuicui Zhang, Yingwu Liang, Weining Tang
Medulloblastoma is the most common malignant brain tumor of childhood, with great potential to metastasize. However, the mechanisms of how medulloblastoma develops and progresses remain to be elucidated. The present study assessed the role of long noncoding RNA LOXL1-AS1 (lncRNA LOXL1-AS1) in the cell proliferation and metastasis in human medulloblastoma. It was initially found that LOXL1-AS1 was significantly overexpressed in clinical medulloblastoma tissues compared with the adjacent noncancerous tissues...
2018: Analytical Cellular Pathology (Amsterdam)
Marwa Al Hassan, Isabelle Fakhoury, Zeinab El Masri, Noura Ghazale, Rayane Dennaoui, Oula El Atat, Amjad Kanaan, Mirvat El-Sibai
Glioblastoma multiforme (GBM) is one of the most common and deadliest cancers of the central nervous system (CNS). GBMs high ability to infiltrate healthy brain tissues makes it difficult to remove surgically and account for its fatal outcomes. To improve the chances of survival, it is critical to screen for GBM-targeted anticancer agents with anti-invasive and antimigratory potential. Metformin, a commonly used drug for the treatment of diabetes, has recently emerged as a promising anticancer molecule. This prompted us, to investigate the anticancer potential of metformin against GBMs, specifically its effects on cell motility and invasion...
2018: Analytical Cellular Pathology (Amsterdam)
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