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Biomedical Optics Express

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https://www.readbyqxmd.com/read/28271016/x-ray-phase-microtomography-with-a-single-grating-for-high-throughput-investigations-of-biological-tissue
#1
Marie-Christine Zdora, Joan Vila-Comamala, Georg Schulz, Anna Khimchenko, Alexander Hipp, Andrew C Cook, Daniel Dilg, Christian David, Christian Grünzweig, Christoph Rau, Pierre Thibault, Irene Zanette
The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271015/lactate-based-optical-screening-of-dengue-virus-infection-in-human-sera-using-raman-spectroscopy
#2
Muhammad Bilal, Rahat Ullah, Saranjam Khan, Hina Ali, Muhammad Saleem, Mushtaq Ahmed
This study presents the screening of dengue virus (DENV) infection in human blood sera based on lactate concentration using Raman spectroscopy. A total of 70 samples, 50 from confirmed DENV infected patients and 20 from healthy volunteers have been used in this study. Raman spectra of all these samples have been acquired in the spectral range from 600 cm(-1) to 1800 cm(-1) using a 532 nm laser as an excitation source. Spectra of all these samples have been analyzed for assessing the biochemical changes resulting from infection...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271014/cmos-based-image-cytometry-for-detection-of-phytoplankton-in-ballast-water
#3
J M Pérez, M Jofre, P Martínez, M A Yáñez, V Catalan, A Parker, M Veldhuis, V Pruneri
We introduce an image cytometer (I-CYT) for the analysis of phytoplankton in fresh and marine water environments. A linear quantification of cell numbers was observed covering several orders of magnitude using cultures of Tetraselmis and Nannochloropsis measured by autofluorescence in a laboratory environment. We assessed the functionality of the system outside the laboratory by phytoplankton quantification of samples taken from a marine water environment (Dutch Wadden Sea, The Netherlands) and a fresh water environment (Lake Ijssel, The Netherlands)...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271013/ultrahigh-resolution-oct-imaging-of-the-human-cornea
#4
René M Werkmeister, Sabina Sapeta, Doreen Schmidl, Gerhard Garhöfer, Gerald Schmidinger, Valentin Aranha Dos Santos, Gerold C Aschinger, Isabella Baumgartner, Niklas Pircher, Florian Schwarzhans, Anca Pantalon, Harminder Dua, Leopold Schmetterer
We present imaging of corneal pathologies using optical coherence tomography (OCT) with high resolution. To this end, an ultrahigh-resolution spectral domain OCT (UHR-OCT) system based on a broad bandwidth Ti:sapphire laser is employed. With a central wavelength of 800 nm, the imaging device allows to acquire OCT data at the central, paracentral and peripheral cornea as well as the limbal region with 1.2 µm x 20 µm (axial x lateral) resolution at a rate of 140 000 A-scans/s. Structures of the anterior segment of the eye, not accessible with commercial OCT systems, are visualized...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271012/deep-feature-learning-for-automatic-tissue-classification-of-coronary-artery-using-optical-coherence-tomography
#5
Atefeh Abdolmanafi, Luc Duong, Nagib Dahdah, Farida Cheriet
Kawasaki disease (KD) is an acute childhood disease complicated by coronary artery aneurysms, intima thickening, thrombi, stenosis, lamellar calcifications, and disappearance of the media border. Automatic classification of the coronary artery layers (intima, media, and scar features) is important for analyzing optical coherence tomography (OCT) images recorded in pediatric patients. OCT has been known as an intracoronary imaging modality using near-infrared light which has recently been used to image the inner coronary artery tissues of pediatric patients, providing high spatial resolution (ranging from 10 to 20 μm)...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271011/optical-coherence-elastography-oct-at-work-in-tissue-biomechanics-invited
#6
REVIEW
Kirill V Larin, David D Sampson
Optical coherence elastography (OCE), as the use of OCT to perform elastography has come to be known, began in 1998, around ten years after the rest of the field of elastography - the use of imaging to deduce mechanical properties of tissues. After a slow start, the maturation of OCT technology in the early to mid 2000s has underpinned a recent acceleration in the field. With more than 20 papers published in 2015, and more than 25 in 2016, OCE is growing fast, but still small compared to the companion fields of cell mechanics research methods, and medical elastography...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271010/measurement-of-dynamic-cell-induced-3d-displacement-fields-in-vitro-for-traction-force-optical-coherence-microscopy
#7
Jeffrey A Mulligan, François Bordeleau, Cynthia A Reinhart-King, Steven G Adie
Traction force microscopy (TFM) is a method used to study the forces exerted by cells as they sense and interact with their environment. Cell forces play a role in processes that take place over a wide range of spatiotemporal scales, and so it is desirable that TFM makes use of imaging modalities that can effectively capture the dynamics associated with these processes. To date, confocal microscopy has been the imaging modality of choice to perform TFM in 3D settings, although multiple factors limit its spatiotemporal coverage...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271009/label-free-cell-substrate-adhesion-imaging-on-plasmonic-nanocup-arrays
#8
L P Hackett, S Seo, S Kim, L L Goddard, G L Liu
Cell adhesion is a crucial biological and biomedical parameter defining cell differentiation, cell migration, cell survival, and state of disease. Because of its importance in cellular function, several tools have been developed in order to monitor cell adhesion in response to various biochemical and mechanical cues. However, there remains a need to monitor cell adhesion and cell-substrate separation with a method that allows real-time measurements on accessible equipment. In this article, we present a method to monitor cell-substrate separation at the single cell level using a plasmonic extraordinary optical transmission substrate, which has a high sensitivity to refractive index changes at the metal-dielectric interface...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271008/influence-of-water-content-on-raman-spectroscopy-characterization-of-skin-sample
#9
Soogeun Kim, Kyung Min Byun, Soo Yeol Lee
We report that the Raman spectrum obtained from porcine skin varies significantly with the change of skin water content. At different water contents from 40 to 55 wt.%, the Raman spectra results using confocal Raman spectroscopy show that the spectral variation of porcine skin is highly affected by skin water content. Experimental data are consistent with the Monte Carlo calculation and it is proved that the intensity of the Raman spectrum depends on the angle distribution and collection efficiency of backscattered light from the sample surface for a varied water content...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271007/terahertz-imaging-of-metastatic-lymph-nodes-using-spectroscopic-integration-technique
#10
Jae Yeon Park, Hyuck Jae Choi, Hwayeong Cheon, Seong Whi Cho, Seungkoo Lee, Joo-Hiuk Son
Terahertz (THz) imaging was used to differentiate the metastatic states of frozen lymph nodes (LNs) by using spectroscopic integration technique (SIT). The metastatic states were classified into three groups: healthy LNs, completely metastatic LNs, and partially metastatic LNs, which were obtained from three mice without infection and six mice infected with murine melanoma cells for 30 days and 15 days, respectively. Under histological examination, the healthy LNs and completely metastatic LNs were found to have a homogeneous cellular structure but the partially metastatic LNs had interfaces of the melanoma and healthy tissue...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271006/vcsel-based-swept-source-for-low-cost-optical-coherence-tomography
#11
Sucbei Moon, Eun Seo Choi
We present a novel wavelength-swept laser source for optical coherence tomography (OCT) which is based on the conventional laser diode technology of the vertical-cavity surface-emitting laser (VCSEL). In our self-heating sweep VCSEL (SS-VCSEL), a VCSEL device is simply driven by ramped pulses of currents in direct intensity modulation. The intrinsic property of VCSEL produces a frequency-swept output through the self-heating effect. By the injected current, the temperature of the active region is gradually increased in this effect...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271005/automated-detection-of-dilated-capillaries-on-optical-coherence-tomography-angiography
#12
Changlei Dongye, Miao Zhang, Thomas S Hwang, Jie Wang, Simon S Gao, Liang Liu, David Huang, David J Wilson, Yali Jia
Automated detection and grading of angiographic high-risk features in diabetic retinopathy can potentially enhance screening and clinical care. We have previously identified capillary dilation in angiograms of the deep plexus in optical coherence tomography angiography as a feature associated with severe diabetic retinopathy. In this study, we present an automated algorithm that uses hybrid contrast to distinguish angiograms with dilated capillaries from healthy controls and then applies saliency measurement to map the extent of the dilated capillary networks...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271004/influence-of-wave-front-sampling-in-adaptive-optics-retinal-imaging
#13
Marie Laslandes, Matthias Salas, Christoph K Hitzenberger, Michael Pircher
A wide range of sampling densities of the wave-front has been used in retinal adaptive optics (AO) instruments, compared to the number of corrector elements. We developed a model in order to characterize the link between number of actuators, number of wave-front sampling points and AO correction performance. Based on available data from aberration measurements in the human eye, 1000 wave-fronts were generated for the simulations. The AO correction performance in the presence of these representative aberrations was simulated for different deformable mirror and Shack Hartmann wave-front sensor combinations...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271003/optical-coherence-tomography-based-angiography-invited
#14
REVIEW
Chieh-Li Chen, Ruikang K Wang
Optical coherence tomography (OCT)-based angiography (OCTA) provides in vivo, three-dimensional vascular information by the use of flowing red blood cells as intrinsic contrast agents, enabling the visualization of functional vessel networks within microcirculatory tissue beds non-invasively, without a need of dye injection. Because of these attributes, OCTA has been rapidly translated to clinical ophthalmology within a short period of time in the development. Various OCTA algorithms have been developed to detect the functional micro-vasculatures in vivo by utilizing different components of OCT signals, including phase-signal-based OCTA, intensity-signal-based OCTA and complex-signal-based OCTA...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271002/coherence-properties-of-short-cavity-swept-lasers
#15
Bart Johnson, Walid Atia, Mark Kuznetsov, Brian D Goldberg, Peter Whitney, Dale C Flanders
It has been shown theoretically and experimentally that short cavity swept lasers are passively mode locked. We develop a mathematical model of these lasers and the light field solutions are used to predict the coherence length and coherence revival behavior. The calculations compare favorably with data from a 990-1100 nm laser swept at 100 kHz suitable for optical coherence tomography applications.
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271001/fully-integrated-optical-coherence-tomography-ultrasound-and-indocyanine-green-based-fluorescence-tri-modality-system-for-intravascular-imaging
#16
Yan Li, Joseph Jing, Yueqiao Qu, Yusi Miao, Buyun Zhang, Teng Ma, Mingyue Yu, Qifa Zhou, Zhongping Chen
We present a tri-modality imaging system and fully integrated tri-modality probe for intravascular imaging. The tri-modality imaging system is able to simultaneously acquire optical coherence tomography (OCT), ultrasound (US), and fluorescence imaging. Moreover, for fluorescence imaging, we used the FDA-approved indocyanine green (ICG) dye as the contrast agent to target lipid-loaded macrophages. We conducted imaging from a male New Zealand white rabbit to evaluate the performance of the tri-modality system...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28271000/core-shell-nanofiber-characterization-by-raman-scanning-microscopy
#17
Lauren Sfakis, Anna Sharikova, David Tuschel, Felipe Xavier Costa, Melinda Larsen, Alexander Khmaladze, James Castracane
Core/shell nanofibers are becoming increasingly popular for applications in tissue engineering. Nanofibers alone provide surface topography and increased surface area that promote cellular attachment; however, core/shell nanofibers provide the versatility of incorporating two materials with different properties into one. Such synthetic materials can provide the mechanical and degradation properties required to make a construct that mimics in vivo tissue. Many variations of these fibers can be produced. The challenge lies in the ability to characterize and quantify these nanofibers post fabrication...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28270999/fully-automated-diagnosis-of-papilledema-through-robust-extraction-of-vascular-patterns-and-ocular-pathology-from-fundus-photographs
#18
Khush Naseeb Fatima, Taimur Hassan, M Usman Akram, Mahmood Akhtar, Wasi Haider Butt
Rapid development in the field of ophthalmology has increased the demand of computer aided diagnosis of various eye diseases. Papilledema is an eye disease in which the optic disc of the eye is swelled due to an increase in intracranial pressure. This increased pressure can cause severe encephalic complications like abscess, tumors, meningitis or encephalitis, which may lead to a patient's death. Although there have been several papilledema case studies reported from a medical point of view, only a few researchers have presented automated algorithms for this problem...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28270998/ultra-fast-line-field-low-coherence-holographic-elastography-using-spatial-phase-shifting
#19
Chih-Hao Liu, Alexander Schill, Raksha Raghunathan, Chen Wu, Manmohan Singh, Zhaolong Han, Achuth Nair, Kirill V Larin
Optical coherence elastography (OCE) is an emerging technique for quantifying tissue biomechanical properties. Generally, OCE relies on point-by-point scanning. However, long acquisition times make point-by-point scanning unfeasible for clinical use. Here we demonstrate a noncontact single shot line-field low coherence holography system utilizing an automatic Hilbert transform analysis based on a spatial phase shifting technique. Spatio-temporal maps of elastic wave propagation were acquired with only one air-pulse excitation and used to quantify wave velocity and sample mechanical properties at a line rate of 200 kHz...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28270997/in-vivo-imaging-of-hepatic-hemodynamics-and-light-scattering-property-during-ischemia-reperfusion-in-rats-based-on-spectrocolorimetry
#20
Sharmin Akter, Satoko Kawauchi, Shunichi Sato, Suefumi Aosasa, Junji Yamamoto, Izumi Nishidate
A red-green-blue camera-based imaging method is proposed for estimating spatial maps of concentrations of oxyhemoglobin (CHbO), deoxyhemoglobin (CHbR), total hemoglobin (CHbT), tissue oxygen saturation (StO2), and scattering power (b) in liver tissue. Hemodynamic responses to hepatic ischemia-reperfusion of in vivo rat liver tissues induced by portal triad occlusion were evaluated. Upon portal triad occlusion, this method yielded images of decreased CHbO, CHbT, StO2, and b, and increased CHbR followed by a progressive increase in CHbO and StO2 during reperfusion...
February 1, 2017: Biomedical Optics Express
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