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Protein & Cell

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https://www.readbyqxmd.com/read/28434148/advancing-chimeric-antigen-receptor-t-cell-therapy-with-crispr-cas9
#1
REVIEW
Jiangtao Ren, Yangbing Zhao
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (CRISPR/Cas9) system, an RNA-guided DNA targeting technology, is triggering a revolution in the field of biology. CRISPR/Cas9 has demonstrated great potential for genetic manipulation. In this review, we discuss the current development of CRISPR/Cas9 technologies for therapeutic applications, especially chimeric antigen receptor (CAR) T cell-based adoptive immunotherapy. Different methods used to facilitate efficient CRISPR delivery and gene editing in T cells are compared...
April 22, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28434147/increasing-the-safety-and-efficacy-of-chimeric-antigen-receptor-t-cell-therapy
#2
REVIEW
Hua Li, Yangbing Zhao
Chimeric antigen receptor (CAR) T cell therapy is a promising cancer treatment that has recently been undergoing rapid development. However, there are still some major challenges, including precise tumor targeting to avoid off-target or "on-target/off-tumor" toxicity, adequate T cell infiltration and migration to solid tumors and T cell proliferation and persistence across the physical and biochemical barriers of solid tumors. In this review, we focus on the primary challenges and strategies to design safe and effective CAR T cells, including using novel cutting-edge technologies for CAR and vector designs to increase both the safety and efficacy, further T cell modification to overcome the tumor-associated immune suppression, and using gene editing technologies to generate universal CAR T cells...
April 22, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28434146/differential-regulation-of-h3s10-phosphorylation-mitosis-progression-and-cell-fate-by-aurora-kinase-b-and-c-in-mouse-preimplantation-embryos
#3
Wenzhi Li, Peizhe Wang, Bingjie Zhang, Jing Zhang, Jia Ming, Wei Xie, Jie Na
Coordination of cell division and cell fate is crucial for the successful development of mammalian early embryos. Aurora kinases are evolutionarily conserved serine/threonine kinases and key regulators of mitosis. Aurora kinase B (AurkB) is ubiquitously expressed while Aurora kinase C (AurkC) is specifically expressed in gametes and preimplantation embryos. We found that increasing AurkC level in one blastomere of the 2-cell embryo accelerated cell division and decreasing AurkC level slowed down mitosis. Changing AurkB level had the opposite effect...
April 22, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28434145/mtorc1-signaling-in-hepatic-lipid-metabolism
#4
REVIEW
Jinbo Han, Yiguo Wang
The mechanistic target of rapamycin (mTOR) signaling pathway regulates many metabolic and physiological processes in different organs or tissues. Dysregulation of mTOR signaling has been implicated in many human diseases including obesity, diabetes, cancer, fatty liver diseases, and neuronal disorders. Here we review recent progress in understanding how mTORC1 (mTOR complex 1) signaling regulates lipid metabolism in the liver.
April 22, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28421388/preferential-distribution-of-nuclear-mapk-signal-in-%C3%AE-%C3%AE-core-neurons-during-long-term-memory-consolidation-in-drosophila
#5
LETTER
Wantong Hu, Xuchen Zhang, Lianzhang Wang, Zhong-Jian Liu, Yi Zhong, Qian Li
No abstract text is available yet for this article.
April 19, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28421387/pharmacokinetics-of-monoclonal-antibodies-and-fc-fusion-proteins
#6
REVIEW
Liming Liu
There are many factors that can influence the pharmacokinetics (PK) of a mAb or Fc-fusion molecule with the primary determinant being FcRn-mediated recycling. Through Fab or Fc engineering, IgG-FcRn interaction can be used to generate a variety of therapeutic antibodies with significantly enhanced half-life or ability to remove unwanted antigen from circulation. Glycosylation of a mAb or Fc-fusion protein can have a significant impact on the PK of these molecules. mAb charge can be important and variants with pI values of 1-2 unit difference are likely to impact PK with lower pI values being favorable for a longer half-life...
April 19, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28405828/copy-number-variants-of-abcf1-il17rel-and-fcgr3a-are-associated-with-the-risk-of-gout
#7
LETTER
Zheng Dong, Yuan Li, Jingru Zhou, Shuai Jiang, Yi Wang, Yulin Chen, Dongbao Zhao, Chengde Yang, Qiaoxia Qian, Yanyun Ma, Hongjun He, Hengdong Ji, Yajun Yang, Xiaofeng Wang, Xia Xu, Yafei Pang, Hejian Zou, Li Jin, Feng Zhang, Jiucun Wang
No abstract text is available yet for this article.
April 12, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28401346/crispr-cas9-mediated-targeted-gene-correction-in-amyotrophic-lateral-sclerosis-patient-ipscs
#8
Lixia Wang, Fei Yi, Lina Fu, Jiping Yang, Si Wang, Zhaoxia Wang, Keiichiro Suzuki, Liang Sun, Xiuling Xu, Yang Yu, Jie Qiao, Juan Carlos Izpisua Belmonte, Ze Yang, Yun Yuan, Jing Qu, Guang-Hui Liu
Amyotrophic lateral sclerosis (ALS) is a complex neurodegenerative disease with cellular and molecular mechanisms yet to be fully described. Mutations in a number of genes including SOD1 and FUS are associated with familial ALS. Here we report the generation of induced pluripotent stem cells (iPSCs) from fibroblasts of familial ALS patients bearing SOD1 (+/A272C) and FUS (+/G1566A) mutations, respectively. We further generated gene corrected ALS iPSCs using CRISPR/Cas9 system. Genome-wide RNA sequencing (RNA-seq) analysis of motor neurons derived from SOD1 (+/A272C) and corrected iPSCs revealed 899 aberrant transcripts...
April 11, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28397077/investigation-on-natural-resources-and-species-conservation-of-ophiocordyceps-sinensis-the-famous-medicinal-fungus-endemic-to-the-tibetan-plateau
#9
Wenjing Wang, Ke Wang, Xiaoliang Wang, Ruiheng Yang, Yi Li, Yijian Yao
No abstract text is available yet for this article.
April 10, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28389873/molecular-barriers-to-direct-cardiac-reprogramming
#10
REVIEW
Haley Vaseghi, Jiandong Liu, Li Qian
Myocardial infarction afflicts close to three quarters of a million Americans annually, resulting in reduced heart function, arrhythmia, and frequently death. Cardiomyocyte death reduces the heart's pump capacity while the deposition of a non-conductive scar incurs the risk of arrhythmia. Direct cardiac reprogramming emerged as a novel technology to simultaneously reduce scar tissue and generate new cardiomyocytes to restore cardiac function. This technology converts endogenous cardiac fibroblasts directly into induced cardiomyocyte-like cells using a variety of cocktails including transcription factors, microRNAs, and small molecules...
April 7, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28386839/erratum-to-guards-at-the-gate-physiological-and-pathological-roles-of-tissue-resident-innate-lymphoid-cells-in-the-lung
#11
Hang Cheng, Chengyan Jin, Jing Wu, Shan Zhu, Yong-Jun Liu, Jingtao Chen
No abstract text is available yet for this article.
April 6, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28364280/oncogenic-mir-19a-and-mir-19b-co-regulate-tumor-suppressor-mtus1-to-promote-cell-proliferation-and-migration-in-lung-cancer
#12
Yuanyuan Gu, Shuoxin Liu, Xiaodan Zhang, Guimin Chen, Hongwei Liang, Mengchao Yu, Zhicong Liao, Yong Zhou, Chen-Yu Zhang, Tao Wang, Chen Wang, Junfeng Zhang, Xi Chen
MTUS1 (microtubule-associated tumor suppressor 1) has been identified that can function as a tumor suppressor gene in many malignant tumors. However, the function and mechanisms underlying the regulation of MTUS1 are unclear. In the present study, we reported that miR-19a and miR-19b (miR-19a/b) promote proliferation and migration of lung cancer cells by targeting MTUS1. First, MTUS1 was proved to function as a tumor suppressor in lung cancer and was linked to cell proliferation and migration promotion. Second, an inverse correlation between miR-19a/b expression and MTUS1 mRNA/protein expression was noted in human lung cancer tissues...
March 31, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28364279/trpv1-and-trpa1-in-cutaneous-neurogenic-and-chronic-inflammation-pro-inflammatory-response-induced-by-their-activation-and-their-sensitization
#13
REVIEW
Olivier Gouin, Killian L'Herondelle, Nicolas Lebonvallet, Christelle Le Gall-Ianotto, Mehdi Sakka, Virginie Buhé, Emmanuelle Plée-Gautier, Jean-Luc Carré, Luc Lefeuvre, Laurent Misery, Raphaele Le Garrec
Cutaneous neurogenic inflammation (CNI) is inflammation that is induced (or enhanced) in the skin by the release of neuropeptides from sensory nerve endings. Clinical manifestations are mainly sensory and vascular disorders such as pruritus and erythema. Transient receptor potential vanilloid 1 and ankyrin 1 (TRPV1 and TRPA1, respectively) are non-selective cation channels known to specifically participate in pain and CNI. Both TRPV1 and TRPA1 are co-expressed in a large subset of sensory nerves, where they integrate numerous noxious stimuli...
March 31, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28364278/the-development-and-function-of-dendritic-cell-populations-and-their-regulation-by-mirnas
#14
REVIEW
Haibo Zhou, Li Wu
Dendritic cells (DCs) are important immune cells linking innate and adaptive immune responses. DCs encounter various self and non-self antigens present in the environment and induce different types of antigen specific adaptive immune responses. DCs can be classified into lymphoid tissue-resident DCs, migratory DCs, non-lymphoid resident DCs, and monocyte derived DCs (moDCs). Recent work has also established that DCs consist of developmentally and functionally distinct subsets that differentially regulate T lymphocyte function...
March 31, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28316032/old-master-zhu-in-memory-of-virologist-guan-fu-zhu
#15
Qing Ye, Tao Jiang, Cheng-Feng Qin
No abstract text is available yet for this article.
March 18, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28315159/identification-of-critical-base-pairs-required-for-ctcf-binding-in-motif-m1-and-m2
#16
LETTER
Wufeng Li, Liping Shang, Kaimeng Huang, Jiao Li, Zhibin Wang, Hongjie Yao
No abstract text is available yet for this article.
March 17, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28290053/targeting-cancer-stem-cells-by-using-chimeric-antigen-receptor-modified-t-cells-a-potential-and-curable-approach-for-cancer-treatment
#17
REVIEW
Yelei Guo, Kaichao Feng, Yao Wang, Weidong Han
Cancer stem cells (CSCs), a subpopulation of tumor cells, have self-renewal and multi-lineage differentiation abilities that play an important role in cancer initiation, maintenance, and metastasis. An accumulation of evidence indicates that CSCs can cause conventional therapy failure and cancer recurrence because of their treatment resistance and self-regeneration characteristics. Therefore, approaches that specifically and efficiently eliminate CSCs to achieve a durable clinical response are urgently needed...
March 13, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28290052/genome-wide-in-vivo-screen-identifies-host-molecule-in-promoting-cancer-metastasis
#18
Yan Gu, Yanfang Liu, Xuetao Cao
No abstract text is available yet for this article.
March 13, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28284008/effective-and-persistent-antitumor-activity-of-her2-directed-car-t-cells-against-gastric-cancer-cells-in-vitro-and-xenotransplanted-tumors-in-vivo
#19
Yanjing Song, Chuan Tong, Yao Wang, Yunhe Gao, Hanren Dai, Yelei Guo, Xudong Zhao, Yi Wang, Zizheng Wang, Weidong Han, Lin Chen
Human epidermal growth factor receptor 2 (HER2) proteins are overexpressed in a high proportion of gastric cancer (GC) cases and affect the maintenance of cancer stem cell (CSC) subpopulations, which are used as targets for the clinical treatment of patients with HER2-positive GC. Despite improvements in survival, numerous HER2-positive patients fail treatment with trastuzumab, highlighting the need for more effective therapies. In this study, we generated a novel type of genetically modified human T cells, expressing a chimeric antigen receptor (CAR), and targeting the GC cell antigen HER2, which harbors the CD137 and CD3ζ moieties...
March 10, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28284007/erratum-to-protein-protein-interaction-analysis-in-crude-bacterial-lysates-using-combinational-method-of-19-f-site-specific-incorporation-and-19-f-nmr
#20
Dong Li, Yanan Zhang, Yao He, Chengwei Zhang, Jiefei Wang, Ying Xiong, Longhua Zhang, Yangzhong Liu, Pan Shi, Changlin Tian
No abstract text is available yet for this article.
March 10, 2017: Protein & Cell
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