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International Journal of Biochemistry and Molecular Biology

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https://www.readbyqxmd.com/read/27853625/targeting-of-slug-sensitizes-anaplastic-thyroid-carcinoma-sw1736-cells-to-doxorubicin-via-puma-upregulation
#1
Anbing Dong, Xuelong Jiao, Dong Chen, Fengyun Hao, Kejun Zhang
Anaplastic thyroid carcinoma (ATC) is one of the most aggressive human cancers and often shows resistance to multimodal therapeutic approaches. It has been shown that the transcriptional repressor Slug inhibits the chemotherapeutic agent-induced apoptosis of cancer cells. We evaluated whether targeting of Slug could augment doxorubicin (DOX)-induced apoptosis of ATC cells. We also determined changes in PUMA (p53-upregulated modulator of apoptosis) expression levels to identify possible mechanisms of their combined actions...
2016: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27570640/transient-resistance-to-dna-damaging-agents-is-associated-with-expression-of-micrornas-135b-and-196b-in-human-leukemia-cell-lines
#2
Tsui-Ting Ho, Xiaolong He, Yin-Yuan Mo, William T Beck
The acquisition of resistance to anticancer drugs is widely viewed as a key obstacle to successful cancer therapy. However, detailed knowledge of the initial molecular events in the response of cancer cells to these chemotherapeutic and stress responses, and how these lead to the development of chemoresistance, remains incompletely understood. Using microRNA array and washout and rechallenge experiments, we found that short term treatment of leukemia cells with etoposide led a few days later to transient resistance that was associated with a corresponding transient increase in expression of ABCB1 mRNA, as well as microRNA (miR)-135b and miR-196b...
2016: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27335683/serum-and-glucocorticoid-inducible-kinase-sgk2-stimulates-the-transport-activity-of-human-organic-anion-transporters-1-by-enhancing-the-stability-of-the-transporter
#3
Da Xu, Haozhe Huang, May Fern Toh, Guofeng You
Human organic anion transporter 1 (hOAT1) belongs to a family of organic anion transporters that play critical roles in the body disposition of clinically important drugs, including anti-viral therapeutics, anti-cancer drugs, antibiotics, antihypertensives, and anti-inflammatories. hOAT1 is abundantly expressed in the kidney and brain. In the current study, we examined the regulation of hOAT1 by serum- and glucocorticoid-inducible kinase 2 (sgk2) in the kidney COS-7 cells. We showed that sgk2 stimulated hOAT1 transport activity...
2016: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27335682/inhibitory-effects-of-chemotherapeutics-on-human-organic-anion-transporter-hoat4
#4
May Fern Toh, Wonmo Suh, Haoxun Wang, Peter Zhou, Longqin Hu, Guofeng You
Human organic anion transporter 4 (hOAT4) belongs to a family of organic anion transporters which play critical roles in the body disposition of clinically important drugs. hOAT4 is expressed in the kidney and placenta. In the current study, we examined the inhibitory effects of 101 anticancer drugs from a clinical drug library on hOAT4 transport activity. The studies were carried out in hOAT4-expressing human kidney HEK-293 cells and human placenta BeWo cells. Among these drugs, only chlorambucil and cabazitaxel demonstrated more than 50% cis-inhibitory effect on hOAT4-mediated uptake of (3)H-labeled estrone sulfate, a prototypical substrate for the transporter...
2016: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27335681/characterization-of-a-starch-hydrolyzing-%C3%AE-amylase-produced-by-aspergillus-niger-wlb42-mutated-by-ethyl-methanesulfonate-treatment
#5
Shihui Wang, Chaoyang Lin, Yun Liu, Zhicheng Shen, Jenasia Jeyaseelan, Wensheng Qin
Aspergillus niger is the most commonly used fungus for commercial amylase production, the increase of amylase activity will be beneficial to the amylase industry. Herein we report a high α-amylase producing (HAP) A. niger WLB42 mutated from A. niger A4 by ethyl methanesulfonate treatment. The fermentation conditions for the amylase production were optimized. The results showed that both the amylase activity and total protein content reached highest after 48-h incubation in liquid medium using starch as the sole carbon source...
2016: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/26823987/establishing-a-cellular-fret-based-fluorescence-plate-reader-assay-to-monitor-prongf-induced-cross-linking-of-sortilin-and-the-neurotrophin-receptor-p75-ntr
#6
Sune Skeldal, Maj M Kjaergaard, Saleh Alwasel, Jens R Nyengaard
Whereas the proform of the nerve growth factor (proNGF) is crucial for eliminating superfluous cells during neuronal development it also promotes apoptosis following brain trauma and neuronal injury. The apoptotic signal is elicited upon formation of a trimeric receptor complex also containing the vps10p domain receptor sortilin and the neurotrophin receptor p75(NTR). However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75(NTR) in live cells...
2015: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/26045972/interleukin-34-induces-monocytic-like-differentiation-in-leukemia-cell-lines
#7
Burthia E Booker, Ryan S Clark, Samuel T Pellom, Samuel E Adunyah
Interleukin-34 (IL-34) is a cytokine consisting of a 39kD homodimer, shown to be a ligand for both the Macrophage Colony Stimulating Factor (M-CSF/CSF-1) receptor and the Receptor-like protein tyrosine phosphatase-zeta (RPTP-ƺ). IL-34 has been shown to promote monocyte viability and proliferation as well as the differentiation of bone marrow cells into macrophage progenitors. Published work on IL-34 involves its effects on normal hematopoietic and osteoclast progenitors. However, it is not known whether IL-34 has biologic effects in cancer, including leukemia...
2015: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24955285/the-interaction-of-the-erbb4-intracellular-domain-p80-with-%C3%AE-enolase-in-the-nuclei-is-associated-with-the-inhibition-of-the-neuregulin1-dependent-cell-proliferation
#8
Satomi Yamada, Masaki Marutsuka, Miyabi Inoue, Jidong Zhang, Shin-Ichi Abe, Ken-Ichi Ishibashi, Naoto Yamaguchi, Ko Eto
We have shown that the receptor tyrosine kinase ErbB4 signals neuregulin1-stimulated proliferation of human cells. Some isoforms of ErbB4 are cleaved to release the soluble intracellular domain p80; however, the function of p80 in cell proliferation remained unclear. Here we propose the possibility for p80 as a negative feedback modulator of ErbB4-mediated cell proliferation. Cells exposed to lower doses of neuregulin1 displayed a stimulated proliferation and contained ErbB4 but barely p80. By contrast, cells exposed to its higher doses displayed a suppressed proliferation and contained p80 but barely ErbB4...
2014: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24955284/transgene-mediated-co-suppression-of-dna-topoisomerase-1-gene-in-caenorhabditis-elegans
#9
Myon-Hee Lee, Dong Seok Cha, Srivalli Swathi Mamillapalli, Young Chul Kwon, Hyeon-Sook Koo
Ectopic expression of multi-transgenic copies can result in reduced expression of the transgene and can induce silence of endogenous gene; this process is called as co-suppression. Using a transgene-mediated co-suppression technique, we demonstrated the biological function of DNA topoisomerase-1 (top-1) in C. elegans development. Introduction of full-length top-1 transgene sufficiently induced the co-suppression of endogenous top-1 gene, causing embryonic lethality and abnormal germline development. We also found that the co-suppression of top-1 gene affected morphogenesis, lifespan and larval growth that were not observed in top-1 (RNAi) animals...
2014: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24955283/role-of-brca1-and-brca2-gene-mutations-in-epithelial-ovarian-cancer-in-indian-population-a-pilot-study
#10
Shikha Sharma, Shalini Rajaram, Tusha Sharma, Neerja Goel, Sarla Agarwal, Basu Dev Banerjee
Ovarian cancer is a silent killer as most patients have non-specific symptoms and usually present in advanced stage of the disease. It occurs due to certain genetic alterations and mutations namely founder mutations, 187delAG and 5385insC in BRCA1 and 6174delT in BRCA2 which are associated with specific family histories. These highly penetrant susceptibility genes responsible for approximately half of families containing 2 or more ovarian cancer cases account for less than 40% of the familial excess malignancy risk...
2014: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24380025/gene-environment-interaction-in-preterm-delivery-with-special-reference-to-organochlorine-pesticide-a-case-control-study
#11
Tusha Sharma, Basu Dev Banerjee, Md Mustafa, Kiran Guleria, Rafat S Ahmed, Ashok K Tripathi
OBJECTIVES: To assess the Gene-Environmental interaction between maternal organochlorine pesticides (OCPs) level and CYP17 gene polymorphism with the risk of preterm delivery (PTD). MATERIALS AND METHODS: Maternal blood samples of hundred cases (n = 100) of PTD and of equal number of healthy controls were collected at the time of delivery. OCPs levels were estimated by Gas chromatography system equipped with electron capture detector and PCR-RFLP was used for polymorphic analysis of CYP17 gene...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24380024/insert-sequence-length-determines-transfection-efficiency-and-gene-expression-levels-in-bicistronic-mammalian-expression-vectors
#12
Andrew J Payne, Bryan C Gerdes, Simon Kaja, Peter Koulen
Bicistronic expression vectors have been widely used for co-expression studies since the initial discovery of the internal ribosome entry site (IRES) about 25 years ago. IRES sequences allow the 5' cap-independent initiation of translation of multiple genes on a single messenger RNA strand. Using a commercially available mammalian expression vector containing an IRES sequence with a 3' green fluorescent protein fluorescent marker, we found that sequence length of the gene of interest expressed 5' of the IRES site influences both expression of the 3' fluorescent marker and overall transfection efficiency of the vector construct...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24380023/caenorhabditis-elegans-neuron-degeneration-and-mitochondrial-suppression-caused-by-selected-environmental-chemicals
#13
Shaoyu Zhou, Zemin Wang, James E Klaunig
Mitochondrial alterations have been documented for many years in the brains of Parkinson's disease (PD), a disorder that is characterized by the selective loss of dopamine neurons. Recent studies have demonstrated that Parkinson's disease-associated proteins are either present in mitochondria or translocated into mitochondria in response to stress, further reinforcing the importance of the mitochondrial function in the pathogenesis of Parkinson's disease. Exposure to environmental chemicals such as pesticides and heavy metals has been suggested as risk factors in the development of Parkinson's disease...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24380022/modulation-of-formation-of-the-3-end-of-the-human-argininosuccinate-synthetase-mrna-by-gt-repeat-polymorphism
#14
Shih-Heng Tseng, Cheng-Yi Cheng, Miao-Zeng Huang, Ming-Yi Chung, Tsung-Sheng Su
Microsatellites are abundant in the human genome and may acquire context-dependent functions. A highly polymorphic GT microsatellite is located downstream of the poly(A) signal of the human argininosuccinate synthetase (ASS1) gene. The ASS1 participates in urea and nitric oxide production and is a rate-limiting enzyme in arginine biosynthesis. To examine possible involvement of the GT microsatellite in ASS1 mRNA 3'-end formation, ASS1 minigene constructs were used in transient transfection for assessment of poly(A) site usage by S1 nuclease mapping...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24380021/structure-of-chitinase-d-from-serratia-proteamaculans-reveals-the-structural-basis-of-its-dual-action-of-hydrolysis-and-transglycosylation
#15
Jogi Madhuprakash, Avinash Singh, Sanjit Kumar, Mau Sinha, Punit Kaur, Sujata Sharma, Appa R Podile, Tej P Singh
Chitinases are known to hydrolyze chitin polymers into smaller chitooligosaccharides. Chitinase from bacterium Serratia proteamaculans (SpChiD) is found to exhibit both hydrolysis and transglycosylation activities. SpChiD belongs to family 18 of glycosyl hydrolases (GH-18). The recombinant SpChiD was crystallized and its three-dimensional structure was determined at 1.49 Å resolution. The structure was refined to an R-factor of 16.2%. SpChiD consists of 406 amino acid residues. The polypeptide chain of SpChiD adopts a (β/α)8 triosephosphate isomerase (TIM) barrel structure...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24380020/functions-of-the-hsp90-chaperone-system-lifting-client-proteins-to-new-heights
#16
REVIEW
Julia M Eckl, Klaus Richter
The molecular chaperone Hsp90 is an essential protein in eukaryotic organisms and is highly conserved throughout all kingdoms of life. It serves as a platform for the folding and maturation of many client proteins including protein kinases and steroid hormone receptors. To fulfill this task Hsp90 performs conformational changes driven by the hydrolysis of ATP. Further, it can resort to a broad set of co-chaperones, which fit the Hsp90 machinery to the needs of specific client proteins. During the last years the number of identified co-chaperones has been consistently rising, implying that the client spectrum of Hsp90 may be much more diverse and larger than currently known...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24049670/the-role-of-calcium-nf-%C3%AE%C2%BAb-and-nfat-in-the-regulation-of-cxcl8-and-il-6-expression-in-jurkat-t-cells
#17
Hazem Khalaf, Jana Jass, Per-Erik Olsson
T-cells play an important role in host immunity against invading pathogens. Determining the underlying regulatory mechanisms will provide a better understanding of T-cell-derived immune responses. In this study, we have shown the differential regulation of IL-6 and CXCL8 by NF-κB and NFAT in Jurkat T-cells, in response to PMA, heat killed Escherichia coli and calcium. CXCL8 was closely associated with the activation pattern of NFAT, while IL-6 expression was associated with NF-κB. Furthermore, increasing the intracellular Ca(2+) concentration by calcium ionophore treatment of the cells resulted in NFAT induction without affecting the NF-κB activity...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24049669/redundancy-or-specificity-the-role-of-the-cdk-pho85-in-cell-cycle-control
#18
REVIEW
Javier Jiménez, Natalia Ricco, Carmen Grijota-Martínez, Rut Fadó, Josep Clotet
It is generally accepted that progression through the eukaryotic cell cycle is driven by cyclin-dependent kinases (CDKs), which are regulated by interaction with oscillatory expressed proteins called cyclins. CDKs may be separated into 2 categories: essential and non-essential. Understandably, more attention has been focused on essential CDKs because they are shown to control cell cycle progression to a greater degree. After clearly determining the basic and "core" mechanisms of essential CDKs, several questions arise...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24049668/biochemical-and-structural-insights-into-mesotrypsin-an-unusual-human-trypsin
#19
REVIEW
Moh'd A Salameh, Evette S Radisky
Thirty five years ago mesotrypsin was first isolated from the human pancreas. It was described as a minor trypsin isoform with the remarkable property of near total resistance to biological trypsin inhibitors. Another unusual feature of mesotrypsin was discovered later, when it was found that mesotrypsin has defective affinity toward many protein substrates of other trypsins. As the younger sibling of the two major trypsins secreted by the pancreas, cationic and the anionic trypsin, it has been speculated to represent an evolutionary waste with no apparent function...
2013: International Journal of Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/24049667/lactoperoxidase-structural-insights-into-the-function-ligand-binding-and-inhibition
#20
REVIEW
Sujata Sharma, Amit Kumar Singh, Sanket Kaushik, Mau Sinha, Rashmi Prabha Singh, Pradeep Sharma, Harshverdhan Sirohi, Punit Kaur, Tej P Singh
Lactoperoxidase (LPO) is a member of a large group of mammalian heme peroxidases that include myeloperoxidase (MPO), eosinophil peroxidase (EPO) and thyroid peroxidase (TPO). The LPO is found in exocrine secretions including milk. It is responsible for the inactivation of a wide range of micro-organisms and hence, is an important component of defense mechanism in the body. With the help of hydrogen peroxide, it catalyzes the oxidation of halides, pseudohalides and organic aromatic molecules. Historically, LPO was isolated in 1943, nearly seventy years ago but its three-dimensional crystal structure has been elucidated only recently...
2013: International Journal of Biochemistry and Molecular Biology
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