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Experimental and Therapeutic Medicine

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https://www.readbyqxmd.com/read/30405748/expression-of-somatostatin-and-its-receptor-1-5-in-endometriotic-tissues-and-cells
#1
Yanhua Zhao, Lin Peng, Xiang Li, Yi Zhang
The present study aimed to detect the expression of somatostatin (SS) and SS receptor (SSTR)1-5 in tissues from patients with endometriosis (EMS). Reverse transcription-quantitative polymerase chain reaction analysis was applied to examine the expression of somatostatin gene in ectopic endometrial cells (EECs). The expression of somatostatin receptor 1-5 in the ectopic endometrium (EE), eutopic endometrium and normal endometrium and their association with EMS staging were determined by immunohistochemistry...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402165/anti-inflammatory-effect-of-resolvin-d1-on-lps-treated-mg-63-cells
#2
Dan Cao, Jing Pi, Yihong Shan, Yuping Tang, Ping Zhou
Inflammation reaction plays an important role in the pathogenesis of ankle fracture. The aim of the present study was to investigate the effect of RvD1 on the inflammatory response and underlying molecular mechanisms in MG-63 cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and ELISA were used to determine the mRNA and protein expression of cytokines extracted from peripheral blood in children with or without ankle fracture, respectively. MG-63 cells were pre-treated with/without RvD1 and stimulated with 1 µg/ml LPS...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402164/optimal-sampling-time-point-for-cyclosporin-a-concentration-monitoring-in-heart-transplant-recipients
#3
Yixin Jia, Xu Meng, Yan Li, Chunlei Xu, Wen Zeng, Yuqing Jiao, Wei Han
The present study was performed to determine an optimal time-point for monitoring the concentration of the immunosuppressive drug cyclosporin A (CsA) in heart transplant patients and its efficacy in the prevention of transplant rejection. A total of 32 transplant recipients were randomly assigned for three treatment approaches. Recipients in groups A (n=11), B (n=13) and C (n=8) received oral administration of CsA at doses of 3.2, 3.5 and 4.4 mg/kg, respectively. The plasma CsA concentrations were examined at 2 h intervals over 12 h...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402163/electrocardiography-gated-dual-source-computed-tomography-in-the-detection-of-atrial-septal-aneurysm
#4
Lixiang Xie, Zhongxiao Liu, Xiuli Zhang, Kai Xu, Qian Xu, Li Lu, Chunfeng Hu, Shuguang Han, Jingjing Li
The aim of the current study was to investigate the atrial septal aneurysm (ASA) incidence rate and describe its morphologic features by dual-source computed tomography (DSCT). A total of 8,626 patients (4,284 men and 4,342 women) who underwent DSCT examinations were enrolled. The presence of ASA was defined as the protrusion of the complete or partial interatrial septum with a maximum vertical distance (MVD) from the atrial septum plane of >10 mm during a cardiac cycle and a diameter of the aneurysm base of >15 mm...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402162/down-regulated-g-protein-coupled-receptor-kinase-6-leads-to-apoptosis-in-multiple-myeloma-mm1r-cells
#5
Zhiyao Zhang, Zhenyu Li, Wenming Chen
G protein-coupled receptor kinase 6 (GRK6) is highly expressed in multiple myeloma (MM) cell lines, but absent or only weakly expressed in most primary human somatic cells. In the present study, GRK6 expression was assessed in MM patients and healthy individuals by quantitative polymerase chain reaction. Flow cytometry were performed to measure the apoptosis of lentivial-transfected MM1R cells. Western blot analysis was performed to assess the apoptosis and signal transducer and activator of transcription 3 pathway-related factors...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402161/mir-365b-3p-inhibits-the-cell-proliferation-and-migration-of-human-coronary-artery-smooth-muscle-cells-by-directly-targeting-adamts1-in-coronary-atherosclerosis
#6
Yunfei Qu, Ning Zhang
Abnormal proliferation and migration of vascular smooth muscle cells serves a crucial role in the development of atherosclerosis. Previous studies have suggested that some microRNAs (miRs) are involved in this process; however, the associated underlying molecular mechanism is unclear. In present study, human coronary artery smooth muscle cells (HCASMCs) were used to explore the function of miR-365b-3p in the coronary atherosclerosis. It was indicated that platelet-derived growth factor-BB (PDGF-BB) treatment inhibited miR-365b-3p expression and upregulated the expression of a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) in HCASMCs...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402160/distinguishing-the-dominant-species-of-pathogen-in-ethmoidal-sinusitis-by-sequencing-dna-dataset-analysis
#7
Junyi Zhang, Shuai He, Yunchuan Li, Minggang Lv, Hongzheng Wei, Bin Qu, Yani Zheng, Chunhua Hu
Identifying the predominant microbial species in patients with ethmoidal sinusitis is conducive to its successful treatment. The aim of the present study was to determine the microbial composition and the predominant fungal and bacterial species in patients with ethmoidal sinusitis. A sample was obtained from 3 patients with ethmoidal sinusitis and from the ethmoid sinus of 2 healthy volunteers. Those samples were sequenced using an Illumina/Solexa sequencing platform for mapping to human, fungal, and bacterial genomes...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402159/imb-6g-induces-endoplasmic-reticulum-stress-mediated-apoptosis-in-human-nasopharyngeal-carcinoma-cells
#8
Yeting Pan, Yanni Zhang, Liang Gong, Jianding Zou, Boxia Hu, Sicong Zhang
IMB-6G is a novel N-substituted sophoridine acid that has been reported to have anticancer effects. The purpose of the present study was to investigate the effect and underlying mechanism of IMB-6G on human nasopharyngeal carcinoma (NPC) cells. The NPC cell line C666-1 was used in the present study and treated with different concentrations of IMB-6G (0, 1, 2 and 5 µM) for 24 h. Subsequently, cell viability was determined using the Cell Counting kit-8 assay and cell apoptosis was analyzed by performing flow cytometry...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402158/human-nasopharyngeal-carcinoma-can-be-radiosensitized-by-trichosanthin-via-inhibition-of-the-pi3k-pathway
#9
Wen Wang, Bo Tan, Jingwei Zhang, Rongliang Qiu, Xinju Liu, Dongmei Liu, Xue Li, Jianhua Wang
Nasopharyngeal carcinoma (NPC) is a prevalent tumor that affects the head and neck. Radiation therapy is typically used to treat NPC; however, poor prognoses and distant metastases are common due to radiation resistance. The antitumor activities of trichosanthin (TCS) have been reported in several types of tumors. The aim of the present study was to investigate whether TCS may serve as a potential radiosensitizer in the treatment of NPC tumors. In the present study, NPC cells were treated with radiation alone or together with TCS and radiosensitivity was compared...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402157/correlation-of-vascular-endothelial-function-and-coagulation-factors-with-renal-function-and-inflammatory-factors-in-patients-with-diabetic-nephropathy
#10
Jie Sun, Caiyan Liu
Correlation of vascular endothelial function and coagulation factors with renal function and inflammatory factors in patients with diabetic nephropathy was analyzed. A total of 86 patients diagnosed with diabetes mellitus (DM) and admitted to the 89th Hospital of the People's Liberation Army (Weifang, China) from March 2014 to May 2017 were selected. Among them, 38 patients complicated with nephropathy were divided into the observation group and 48 patients without nephropathy into the control group. The general data of patients were collected, and the relevant biochemical indexes, vascular endothelial function, coagulation factors and renal function indicators and the levels of inflammatory factors were determined...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402156/outcomes-of-endovascular-interventional-therapy-for-primary-budd-chiari-syndrome-caused-by-hepatic-venous-obstruction
#11
De-Lei Cheng, Nan Zhu, Hao Xu, Cheng-Li Li, Wei-Fu Lv, Wei-Wei Fang, Chuan-Ting Li
To date, interventional therapy for patients with Budd-Chiari syndrome (BCS) due to hepatic vein obstruction (HVO) has not been standardized in China. In Western countries, BCS primarily occurs due to thrombosis and the majority of patients receive thrombolysis. In China, BCS is mostly caused by the membranous occlusion of the HV or IVC. The present retrospective study evaluated the efficacy of recanalization techniques in patients with primary BCS due to HVO. The data of 69 patients with BCS due to HVO, who underwent endovascular therapy at 2 centers in China between December 2010 and December 2012, were analyzed...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402155/comparison-of-the-predictive-value-of-four-dimensional-speckle-tracking-imaging-risk-classification-and-the-timi-system-after-stemi-reperfusion-therapy
#12
Yingchun Wang, Rong Xu, Xiaohua Yin, Jing Wang, Lan Feng, Jie Zhou
The predictive value of four-dimensional speckle tracking imaging (4D-STI) risk classification and TIMI risk scores for the prognosis of patients with ST-segment elevation myocardial infarction (STEMI) after reperfusion therapy were investigated. One hundred and twenty patients with STEMI after reperfusion therapy were involved. At 12 h after reperfusion therapy, 2nd and 3rd day, the three-dimensional longitudinal strain (LS), circumferential strain (CS) and radial strain (RS), area strain (AS), as well as other 4D-STI detection indicators, were collected...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402154/therapeutic-effect-of-irbesartan-combined-with-atorvastatin-calcium-in-the-treatment-of-rats-with-coronary-heart-disease
#13
Tao Li, Weina Yao
This study aimed to investigate the therapeutic effect of irbesartan combined with atorvastatin calcium in the treatment of rats with coronary heart disease. One hundred sixty Wistar rats were selected to establish coronary heart disease model. Rats with coronary heart disease were randomly divided into 4 groups: Model, irbesartan, atorvastatin calcium and combination groups (irbesartan combined with atorvastatin calcium group). Rats in irbesartan group were treated with 50 mg/(kg.day) irbesartan; rats in atorvastatin calcium group were given atorvastatin calcium at a dose of 10 mg/(kg...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402153/ginsenoside-rg1-attenuates-liver-injury-induced-by-d-galactose-in-mice
#14
Ming-He Xiao, Jie-Yu Xia, Zi-Ling Wang, Wen-Xu Hu, Yan-Ling Fan, Dao-Yong Jia, Jing Li, Peng-Wei Jing, Lu Wang, Ya-Ping Wang
The present study investigated the effect and underlying mechanisms of ginsenoside Rg1 (Rg1) in attenuating subacute liver injury induced by D-galactose (D-gal) in mice. Specific Pathogen Free (SPF) male C57BL/6J mice were randomly divided into 3 groups: i) D-gal-administration group (D-gal group), where the mice were intraperitoneally administrated with D-gal (120 mg/kg/day for 42 days); ii) D-gal + Rg1 group where the mice were treated with 120 mg/kg/day D-gal for 42 days and with Rg1 at a dose of 20 mg/kg/day for 35 days...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402152/calcimimetic-r568-reduced-the-blood-pressure-and-improved-aortic-remodeling-in-spontaneously-hypertensive-rats-by-inhibiting-local-renin-angiotensin-system-activity
#15
Ruixia Sun, Wenwen Zhang, Hua Zhong, Lamei Wang, Na Tang, Yongmin Liu, Yongli Zhao, Tian Zhang, Fang He
Hypertension is a cardiovascular disease that seriously affects human health. Activation of the calcium-sensing receptor (CaSR) inhibits cyclic adenosine monophosphate (cAMP) formation by increasing [Ca2+ ]i and subsequently inhibiting renin release. The renin-angiotensin system (RAS) plays an important role in the development of essential hypertension (EH). The purpose of this study was to determine the effects of NPSR568 (R568)-activated CaSR on blood pressure (BP), proliferation, and remodeling of vascular smooth muscle cells, and the activity of the RAS in spontaneously hypertensive rats (SHRs)...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402151/expression-of-tissue-factor-in-human-cervical-carcinoma-tissue
#16
Xitong Zhao, Chu Cheng, Jinhai Gou, Tao Yi, Yanping Qian, Xue Du, Xia Zhao
The present study aimed to investigate tissue factor (TF) expression in cervical cancer and explore its association with disease progression. A total of 258 cervical cancer tissues and their adjacent normal tissues were collected between September 2014 and September 2016. TF expression was detected in the tissue samples by immunohistochemistry and western blot analysis. Associations between the expression of TF and clinical stage, differentiation status and metastasis of cancer cells were examined. The mean immunohistochemistry score of TF expression in cervical cancer tissues was 2...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402150/intraoperative-and-postoperative-infusion-of-dexmedetomidine-combined-with-intravenous-butorphanol-patient-controlled-analgesia-following-total-hysterectomy-under-laparoscopy
#17
Juan Du, Jian-Wei Li, Jin Jin, Cun-Xian Shi, Jia-Hai Ma
The present prospective, randomized, double-blinded, controlled study aimed to investigate the efficacy and safety of dexmedetomidine (DEX) combined with butorphanol for patient-controlled intravenous analgesia (PCIA) following total laparoscopic hysterectomy. A total of 88 patients undergoing total laparoscopic hysterectomy and receiving postoperative PCIA were divided into two groups following surgery. Patients received DEX 0.5 µg/kg intravenously in the DEX group or 0.9% normal saline in the control (CON) group following anesthesia induction...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402149/protective-effect-of-dexpanthenol-against-cisplatin-induced-hepatotoxicity
#18
Yilmaz Bilgic, Sami Akbulut, Zeynep Aksungur, Mehmet Erman Erdemli, Onural Ozhan, Hakan Parlakpinar, Nigar Vardi, Yusuf Turkoz
The aim of the present study was to investigate the protective effect of dexpanthenol (Dexp) against cisplatin (Cis)-induced hepatotoxicity. Thirty-two Sprague Dawley rats were divided into four groups: Control group (n=8), Dexp group (n=8, 500 mg/kg/ip/daily single dose/3 days Dexp), Cis group (n=8, 7 mg/kg/ip/single dose Cis) and Cis+Dexp group (n=8, 500 mg/kg/ip/daily single dose/3 days Dexp +7 mg/kg/ip/single dose Cis). MDA, CAT, GSH, GSH-Px, TOS, TAS, OSI, Total Nitrit, IL-1β, IL-6 and TNF-α levels were analyzed in liver tissue samples...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402148/enhancement-of-osteogenesis-of-rabbit-bone-marrow-derived-mesenchymal-stem-cells-by-transfection-of-human-bmp-2-and-egfp-recombinant-adenovirus-via-wnt-signaling-pathway
#19
Cheng-Cong Wu, Fang Wang, Shu Rong, Jing Ren, Jian-Shan Wan, Li-Xiang Shi, Zhen Wu, Tao Liu, Qiang Li
Bone marrow mesenchymal stem cells (BMSCs) are considered the most important seed cells in bone tissue engineering. The present study aimed to investigate the potential of rabbit BMSCs in osteogenesis after the transfection of human BMP-2 and EGFP recombinant adenovirus. Rabbit BMSCs were isolated and the surface stem cell makers, including CD29, CD44 and CD45 were detected by flow cytometry. The expression of BMP-2 mRNA and protein in BMSCs were detected by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively...
November 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30402147/role-of-mir-520b-in-non-small-cell-lung-cancer
#20
Linlin Zhang, Shuangquan Yu
The aim of the present study was to investigate the expression of microRNA (miR)-520b in non-small cell lung cancer (NSCLC) and its biological functions. Reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-520b in 52 cases of NSCLC tissues, and its associations with tumor clinical staging and lymph node metastasis were analyzed. miR-520b mimics was transfected into A549 and Calu-3 cells. Cell proliferation, cell cycle, and cell invasion and migration abilities were assessed via cell counting kit-8 assay, flow cytometry and Transwell chamber assay, respectively...
November 2018: Experimental and Therapeutic Medicine
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