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Science China. Life Sciences

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https://www.readbyqxmd.com/read/28616808/crop-3d-a-lidar-based-platform-for-3d-high-throughput-crop-phenotyping
#1
Qinghua Guo, Fangfang Wu, Shuxin Pang, Xiaoqian Zhao, Linhai Chen, Jin Liu, Baolin Xue, Guangcai Xu, Le Li, Haichun Jing, Chengcai Chu
With the growing population and the reducing arable land, breeding has been considered as an effective way to solve the food crisis. As an important part in breeding, high-throughput phenotyping can accelerate the breeding process effectively. Light detection and ranging (LiDAR) is an active remote sensing technology that is capable of acquiring three-dimensional (3D) data accurately, and has a great potential in crop phenotyping. Given that crop phenotyping based on LiDAR technology is not common in China, we developed a high-throughput crop phenotyping platform, named Crop 3D, which integrated LiDAR sensor, high-resolution camera, thermal camera and hyperspectral imager...
December 6, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28914434/human-infection-with-a-further-evolved-avian-h9n2-influenza-a-virus-in-sichuan-china
#2
LETTER
Jianan Xu, Shuang Li, Yong Yang, Bing Liu, Huiping Yang, Tiansu Li, Lin Zhang, Wei Li, Xiaohu Luo, Liquan Zhang, Ming Pan
No abstract text is available yet for this article.
September 13, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28914433/platelet-transfusion-refractoriness-after-t-cell-replete-haploidentical-transplantation-is-associated-with-inferior-clinical-outcomes
#3
Qiang Fu, Lanping Xu, Xiaohui Zhang, Yu Wang, Yingjun Chang, Kaiyan Liu, Xiaojun Huang
Haploidentical stem cell transplantation (haplo-SCT) has been an alternative source of bone marrow for patients without human leukocyte antigen (HLA)-matched donors. The aim of this study was to investigate the relationships between platelet transfusion refractoriness (PTR) and clinical outcomes in the setting of haplo-SCT. Between May 2012 and March 2014, 345 patients who underwent unmanipulated haplo-SCT were retrospectively enrolled. PTR occurred in 20.6% of all patients. Patients in the PTR group experienced higher transplant-related mortality (TRM, 43...
September 13, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28895115/sumoylation-of-suvr2-contributes-to-its-role-in-transcriptional-gene-silencing
#4
Yu-Xi Luo, Yong-Feng Han, Qiu-Yuan Zhao, Jin-Lu Du, Kun Dou, Lin Li, She Chen, Xin-Jian He
The SU(VAR)-3-9-related protein family member SUVR2 has been previously identified to be involved in transcriptional gene silencing both in RNA-dependent and -independent pathways. It interacts with the chromatin-remodeling proteins CHR19, CHR27, and CHR28 (CHR19/27/28), which are also involved in transcriptional gene silencing. Here our study demonstrated that SUVR2 is almost fully mono-sumoylated in vivo. We successfully identified the exact SUVR2 sumoylation site by combining in vitro mass spectrometric analysis and in vivo immunoblotting confirmation...
September 8, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28887625/overexpressed-brh1-a-ring-finger-gene-alters-rosette-leaf-shape-in-arabidopsis-thaliana
#5
Xiaoqian Wang, Eryong Chen, Xiaoyang Ge, Qian Gong, HamamaIslam Butt, Chaojun Zhang, Zuoren Yang, Fuguang Li, Xueyan Zhang
Leaves are the most important plant parts for photosynthesis and respiration. Many genes are involved in determining leaf shape; however, little is known about the effects of brassinosteroid (BR) signaling-pathway genes on the development of leaf shape. Here, the brassinosteroid-responsive RING-H2 (BRH1) gene, which is suppressed by 24-epi-brassinolide treatment, was isolated from Arabidopsis thaliana. The amino acid sequence contained a highly conserved RING finger domain. In a phylogenetic analysis, BRH1 clustered closely with GLYMA11G02470...
September 7, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28887624/china-in-action-national-strategies-to-combat-against-emerging-infectious-diseases
#6
Min Han, Jinhui Gu, George F Gao, William J Liu
No abstract text is available yet for this article.
September 7, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28861868/photoperiodic-refractoriness-in-energy-balance-a-specific-pattern-in-mongolian-gerbils-meriones-unguiculatus
#7
LETTER
Wei Yao, Dehua Wang, Xueying Zhang
No abstract text is available yet for this article.
August 29, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28861867/urine-glucose-levels-are-disordered-before-blood-glucose-level-increase-was-observed-in-zucker-diabetic-fatty-rats
#8
Wei Yin, Weiwei Qin, Youhe Gao
Many patients with diabetes are not diagnosed at all or are diagnosed too late to be effectively treated, resulting in nonspecific symptoms and a long period of incubation of the disease. Pre-diabetes is an early warning signal of diabetes, and the change of urine glucose in this period has been ignored even though urine has long been related with diabetes. In this study, Zucker diabetic fatty (ZDF) rats were used to test if there were changes in urine glucose before blood glucose increases. Six 8-week-old male ZDF rats (fa/fa) and Zucker lean (ZL) rats (fa/+) were fed with Purina 5008 high-fat diet and tested for fasting blood glucose and urine glucose...
August 29, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28861869/a-comparative-study-of-distant-hybridization-in-plants-and-animals
#9
REVIEW
Jie Chen, Mi Luo, Shengnan Li, Min Tao, Xiaolan Ye, Wei Duan, Chun Zhang, Qinbo Qin, Jun Xiao, Shaojun Liu
Distant hybridization refers to crosses between two different species, genera, or higher-ranking taxa, which can break species limits, increase genetic variation, and combine the biological characteristics of existing species. It is an important way of creating genetic variation, fertile strains, and excellent characteristics in new strains and populations. Combining analyses and summaries from many inter-related documents in plants and animals, both domestic and international, including examples and long-standing research on distant hybridization in fish from our laboratory, we summarize and compare the similarities and differences in plant and animal distant hybridization...
August 28, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28853059/synaptobrevin-2-facilitates-the-trafficking-and-function-of-atrial-sk2-channel
#10
LETTER
Tao Li, Xuehui Fan, Yiyan Yu, Linlin Chen, Wenjun Huang, Yan Yang, Jimin Cao, Xiaorong Zeng, Xiaoqiu Tan
No abstract text is available yet for this article.
August 25, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28844090/role-of-trim33-in-wnt-signaling-during-mesendoderm-differentiation
#11
Xiaojie Xia, Feifei Zuo, Maoguo Luo, Ye Sun, Jianbo Bai, Qiaoran Xi
Tripartite motif 33 (TRIM33), a member of the transcription intermediate factor 1 (TIF1) family of transcription cofactors, mediates transforming growth factor-beta (TGF-β) signaling through its PHD-Bromo cassette in mesendoderm differentiation during early mouse embryonic development. However, the role of the TRIM33 RING domain in embryonic differentiation is less clear. Here, we report that TRIM33 mediates Wnt signaling by directly regulating the expression of a specific subset of Wnt target genes, and this action is independent of its RING domain...
August 24, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28840534/gut-liver-axis-gut-microbiota-in-shaping-hepatic-innate-immunity
#12
REVIEW
Xunyao Wu, Zhigang Tian
Gut microbiota play an essential role in shaping immune cell responses. The liver was continuously exposed to metabolic products of intestinal commensal bacterial through portal vein and alteration of gut commensal bateria was always associated with increased risk of liver inflammation and autoimmune disease. Considered as a unique immunological organ, the liver is enriched with a large number of innate immune cells. Herein, we summarize the available literature of gut microbiota in shaping the response of hepatic innate immune cells including NKT cells, NK cells, γδ T cells and Kupffer cells during health and disease...
August 22, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28840533/statistical-characteristics-of-forest-litterfall-in-china
#13
LETTER
Bingrui Jia, Zhenzhu Xu, Guangsheng Zhou, Xiaojie Yin
No abstract text is available yet for this article.
August 22, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28840532/reconstruction-of-a-hybrid-nucleoside-antibiotic-gene-cluster-based-on-scarless-modification-of-large-dna-fragments
#14
Jiming Zhuo, Binbin Ma, Jingjing Xu, Weihong Hu, Jihui Zhang, Huarong Tan, Yuqing Tian
Genetic modification of large DNA fragments (gene clusters) is of great importance in synthetic biology and combinatorial biosynthesis as it facilitates rational design and modification of natural products to increase their value and productivity. In this study, we developed a method for scarless and precise modification of large gene clusters by using RecET/RED-mediated polymerase chain reaction (PCR) targeting combined with Gibson assembly. In this strategy, the biosynthetic genes for peptidyl moieties (HPHT) in the nikkomycin biosynthetic gene cluster were replaced with those for carbamoylpolyoxamic acid (CPOAA) from the polyoxin biosynthetic gene cluster to generate a ~40 kb hybrid gene cluster in Escherichia coli with a reusable targeting cassette...
August 21, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28842897/initial-gut-microbiota-structure-affects-sensitivity-to-dss-induced-colitis-in-a-mouse-model
#15
Min Li, Yanqiu Wu, Yingxin Hu, Liping Zhao, Chenhong Zhang
The dextran sulfate sodium (DSS)-induced colitis model is a widely applied mouse model, but controversial results have been obtained from experiments using the same mouse strain under the same conditions. Because the gut microbiota play an important role in DSS-induced colitis, it is essential to evaluate the influence of the initial gut microbiota in this model. Here, we identified significant variations in the initial gut microbiota of different batches of mice and found that the initial intestinal microbiota had a profound influence on DSS-induced colitis...
August 15, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28812299/conversion-of-the-high-yield-salinomycin-producer-streptomyces-albus-bk3-25-into-a-surrogate-host-for-polyketide-production
#16
Xiaojie Zhang, Chenyang Lu, Linquan Bai
An ideal surrogate host for heterologous production of various natural products is expected to have efficient nutrient utilization, fast growth, abundant precursors and energy supply, and a pronounced gene expression. Streptomyces albus BK3-25 is a high-yield industrial strain producing type-I polyketide salinomycin, with a unique ability of bean oil utilization. Its potential of being a surrogate host for heterologous production of PKS was engineered and evaluated herein. Firstly, introduction of a three-gene cassette for the biosynthesis of ethylmalonyl-CoA resulted in accumulation of ethylmalonyl-CoA precursor and salinomycin, and subsequent deletion of the salinomycin biosynthetic gene cluster resulted in a host with rich supplies of common polyketide precursors, including malonyl-CoA, methylmalonyl-CoA, and ethylmalonyl-CoA...
August 14, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28812298/a-gata-type-transcription-factor-acareb-for-nitrogen-metabolism-is-involved-in-regulation-of-cephalosporin-biosynthesis-in-acremonium-chrysogenum
#17
Feifei Guan, Yuanyuan Pan, Jinyang Li, Gang Liu
In filamentous fungi, nitrogen metabolism is repressed by GATA-type zinc finger transcription factors. Nitrogen metabolite repression has been found to affect antibiotic production, but the mechanism is still poorly understood. AcareB, encoding a homologue of fungal GATA-type regulatory protein, was cloned from Acremonium chrysogenum. Gene disruption and genetic complementation demonstrated that AcareB plays a key role in utilization of ammonium, glutamine and urea. In addition, significant reduction of cephalosporin production in the AcareB disruption mutant indicated that AcareB is important for cephalosporin production...
August 14, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28812297/neomycin-biosynthesis-is-regulated-positively-by-afsa-g-and-neor-in-streptomyces-fradiae-cgmcc-4-7387
#18
Xiangxi Meng, Wenzhao Wang, Zhoujie Xie, Pengwei Li, Yue Li, Zhengyan Guo, Yingjian Lu, Jie Yang, Kaile Guan, Zhaoxin Lu, Huarong Tan, Yihua Chen
Neomycins are a group of aminoglycoside antibiotics with both clinical and agricultural applications. To elucidate the regulatory mechanism of neomycin biosynthesis, we completed draft genome sequencing of a neomycin producer Streptomyces fradiae CGMCC 4.7387 from marine sediments, and the neomycin biosynthesis gene cluster was identified. Inactivation of the afsA-g gene encoding a γ-butyrolactone (GBL) synthase in S. fradiae CGMCC 4.7387 resulted in a significant decrease of neomycin production. Quantitative RT-PCR analysis revealed that the transcriptional level of neoR and the aphA-neoGH operon were reduced in the afsA-g::aac(3)IV mutant...
August 14, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28812296/mxr7-facilitates-liver-cancer-metastasis-via-epithelial-mesenchymal-transition
#19
Guishuai Lv, Yexiong Tan, Hongwei Lv, Tian Fang, Changzheng Wang, Ting Li, Yanting Yu, Congli Hu, Wen Wen, Hongyang Wang, Wen Yang
MXR7 is a cell-surface protein and highly expressed in hepatocellular carcinoma (HCC). The aim of this study is to determine the expression profile of MXR7 in HCC and investigate the influence of MXR7 on invasion and metastasis of HCC cells. For this purpose, immunohistochemical assay was used to identify the differential expression of MXR7 in 94 HCC specimens. Expression of MXR7 in 4 pairs of HCC and portal vein tumor thrombus (PVTT) was also tested. The motility of HCC cells were characterized by transwell migration and matrigel invasion assays...
August 11, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28801861/human-piwi-hiwi-is-an-azoospermia-factor
#20
Hidetoshi Hasuwa, Kyoko Ishino, Haruhiko Siomi
No abstract text is available yet for this article.
August 10, 2017: Science China. Life Sciences
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