journal
https://read.qxmd.com/read/37530737/cell-reprogramming-techniques-contributions-to-cancer-therapy
#21
REVIEW
Tongtong Guo, Qi Wei
The reprogramming of terminally differentiated cells over the past few years has become important for induced pluripotent stem cells (iPSCs) in the field of regenerative medicine and disease drug modeling. At the same time, iPSCs have also played an important role in human cancer research. iPSCs derived from cancer patients can be used to simulate the early progression of cancer, for drug testing, and to study the molecular mechanism of cancer occurrence. In recent years, with the application of cellular immunotherapy in cancer therapy, patient-derived iPSC-induced immune cells (T, natural killer, and macrophage cells) solve the problem of immune rejection and have higher immunogenicity, which greatly improves the therapeutic efficiency of immune cell therapy...
August 3, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37590008/a-novel-method-for-human-adipose-derived-stem-cell-isolation-and-cryopreservation
#22
JOURNAL ARTICLE
Young-Cheol Lim, Jung-Il Jung, In-Kee Hong
Adipose-derived stem cells (ADSCs) are isolated from abundant adipose tissue and have the capacity to differentiate into multiple cell lineages. ADSCs have raised big interest in therapeutic applications in regenerative medicine and demonstrated to fulfill the criteria for a successful cell therapy. There are several methods for isolation of ADSCs from adipose tissue and cryopreservation of ADSCs. Here, novel methods for the isolation and cryopreservation of ADSCs are presented and focused. Microscopic pieces of adipose tissue were placed on transwell inserts, and the ADSCs were induced to migrate to the lower wells for 1 week...
August 2023: Cellular Reprogramming
https://read.qxmd.com/read/37590007/unleashing-ascl1-exploring-cross-lineage-potential-in-reprogramming-and-regenerative-frontiers
#23
JOURNAL ARTICLE
Camila Vazquez Echegaray
In the era of stem cell research and regenerative medicine, understanding the regulatory networks that drive cellular reprogramming is fundamental. The study entitled "Cross-lineage potential of Ascl1 uncovered by comparing diverse reprogramming regulatomes" published in Stem Cell Reports sheds light on the remarkable versatility of Ascl1, a transcription factor known for its pivotal role in neurogenesis. By comparing regulatomes across multiple cell lineages, the authors have elucidated the potential of Ascl1 to facilitate the conversion of non-neural cells into various lineages beyond its canonical neural fate, suggesting its potential as a master regulator for lineage reprogramming...
August 2023: Cellular Reprogramming
https://read.qxmd.com/read/37590006/reprogramming-stars-13-establishing-connections-with-cellular-reprogramming-an-interview-with-dr-daniella-rylander-ottosson
#24
JOURNAL ARTICLE
Daniella Rylander Ottosson, Carlos-Filipe Pereira
No abstract text is available yet for this article.
August 2023: Cellular Reprogramming
https://read.qxmd.com/read/37498857/rosalind-franklin-society-proudly-announces-the-2022-award-recipient-for-c-ellular-reprogramming
#25
JOURNAL ARTICLE
Cristiana F Pires
No abstract text is available yet for this article.
July 26, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37471050/cell-transdifferentiation-a-challenging-strategy-with-great-potential
#26
REVIEW
Fuping Wang, Runting Li, Limeng Zhang, Xiaoning Nie, Linqing Wang, Longxin Chen
With the discovery and development of somatic cell nuclear transfer, cell fusion, and induced pluripotent stem cells, cell transdifferentiation research has presented unique advantages and stimulated a heated discussion worldwide. Cell transdifferentiation is a phenomenon by which a cell changes its lineage and acquires the phenotype of other cell types when exposed to certain conditions. Indeed, many adult stem cells and differentiated cells were reported to change their phenotype and transform into other lineages...
July 20, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37384924/transcriptomic-heterogeneity-of-human-mesenchymal-stem-cells-derived-from-bone-marrow-dental-pulp-adipose-tissue-and-umbilical-cord
#27
JOURNAL ARTICLE
Xiaoxiao Zhu, Xinchen Xu, Mengyuan Shen, Yingying Wang, Tao Zheng, Huitao Li, Xing Wang, Jian Meng
Compared with mesenchymal stem cells (MSCs) obtained from other tissue sources, those derived from umbilical cord (UC) tissue exhibit numerous advantages and vast potential for therapeutic applications. However, MSCs from different tissue sources are heterogeneous, and therefore, the therapeutic efficacy of UC-derived MSCs as a replacement for other tissue-derived MSCs needs to be studied. To better understand the distinctions between UC-derived MSCs and MSCs derived from other tissues, we conducted a transcriptome analysis of MSCs obtained from UC and three other tissues...
June 29, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37366790/human-induced-pluripotent-stem-cell-derived-pericytes-as-scalable-and-editable-source-to-study-direct-lineage-reprogramming-into-induced-neurons
#28
JOURNAL ARTICLE
Radhika Menon, Linda Petrucci, Benjamin Lohrer, Jingzhong Zhang, Markus Schulze, Christian Schichor, Beate Winner, Jürgen Winkler, Markus J Riemenschneider, Ralf Kühn, Sven Falk, Marisa Karow
Studying human somatic cell-to-neuron conversion using primary brain-derived cells as starting cell source is hampered by limitations and variations in human biopsy material. Thus, delineating the molecular variables that allow changing the identity of somatic cells, permit adoption of neuronal phenotypes, and foster maturation of induced neurons (iNs) is challenging. Based on our previous results that pericytes derived from the adult human cerebral cortex can be directly converted into iNs (Karow et al., 2018; Karow et al...
June 27, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37327373/a-safer-path-to-cellular-rejuvenation-endogenous-oct4-activation-via-crispr-dcas9-in-progeria-mouse-models
#29
Di Hu, Enora Le Borgne, Rico Meinl
A recent study in Aging Cell showed that transcriptional activation of endogenous Oct4 using the CRISPR/dCas9 activator system is sufficient for cellular rejuvenation and extending the lifespan of a progeria mouse model. Although transient expression of reprogramming factors Oct4, Sox2, Klf4, and c-Myc (OSKM) has been shown to ameliorate age-related phenotypes in vivo , oncogenic risk, for example, from c-Myc, has raised safety concerns for its use in therapeutics. The authors demonstrated that transient activation of endogenous Oct4 expression restored age-related epigenetic patterns, suppressed expression of mutant progerin, and reduced vascular pathological features associated with the disease...
June 16, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37318768/reprogramming-stars-12-at-the-heart-of-in-vivo-reprogramming-an-interview-with-dr-li-qian
#30
JOURNAL ARTICLE
Li Qian, Carlos-Filipe Pereira
No abstract text is available yet for this article.
June 2023: Cellular Reprogramming
https://read.qxmd.com/read/37200520/microrna26a-overexpression-hastens-osteoblast-differentiation-capacity-in-dental-stem-cells
#31
JOURNAL ARTICLE
Steven Kaufman, Peter Chang, Elisha Pendleton, Nalini Chandar
Dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHED) are a source of mesenchymal stem cells with the potential to differentiate into several cell types. We initially isolated SHED cells and compared their osteogenic capacity with commercially available DPSCs. Both cells exhibited similar capacities of growth and osteogenic differentiation. A fourfold to sixfold increase in endogenous microRNA26a (miR26a) expression during osteogenic differentiation of preosteoblasts and a similar but attenuated increase (twofold to fourfold) in differentiating SHED was observed, suggesting a role in the process...
May 18, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37184657/ameliorative-effects-of-extracellular-vesicles-derived-from-mesenchymal-stem-cells-on-apoptosis-and-differentiation-of-osteoblasts-treated-with-cocl-2
#32
JOURNAL ARTICLE
Qicheng Li, Wei Zhang, Jin Deng, Qiuya Li, Xiaoyang Fu, Yuhui Kou, Na Han
Severe osteoporotic fracture occurring in sites with inadequate blood supply can cause irreversible damage to cells, particularly osteoblasts, with current drug and surgical interventions exhibiting limitations for elderly individuals. As participants mediating intercellular communication, extracellular vesicles (EVs) are rarely reported to play functional roles in osteoblasts under hypoxia. Our study mainly investigated the effects of bone marrow mesenchymal stem cells-derived EVs (BMSCs-EVs) on apoptosis and differentiation of osteoblasts treated with CoCl2 ...
May 15, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37172278/functions-of-key-enzymes-of-glycolytic-metabolism-in-tumor-microenvironment
#33
REVIEW
Wenxin Xu, Jialei Weng, Minghao Xu, Qiang Zhou, Shaoqing Liu, Zhiqiu Hu, Ning Ren, Chenhao Zhou, Yinghao Shen
The tumor microenvironment (TME) plays a crucial role in tumor initiation, growth and metastasis. Metabolic enzymes involved in tumor glycolytic reprogramming, including hexokinase, pyruvate kinase, and lactate dehydrogenase, not only play key roles in tumorigenesis and maintaining tumor cell survival, but also take part in the modulation of the TME. Many studies have been devoted to the role of key glycolytic enzymes in the TME over the past decades. We summarize the studies on the role of glycolytic enzymes in the TME of these years and found that glycolytic enzymes remodel the TME primarily through regulating immune escape, angiogenesis, and affecting stromal cells and exosomes...
May 12, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37155628/the-emerging-role-of-b1-sine-in-pluripotent-reprogramming
#34
Jere Weltner, Ras Trokovic
By screening a CRISPR knockout library for mouse pluripotent reprogramming roadblock genes, Kaemena et al. identify the KRAB-ZFP factor Zfp266 as a suppressor of efficient reprogramming. Furthermore, by analyzing DNA binding and chromatin openness, the authors found that ZFP266 has a role in suppressing reprogramming by targeting the B1 SINE sequences for silencing.
May 8, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37042654/production-of-mstn-gene-edited-embryos-of-buffalo-using-the-crispr-cas9-system-and-scnt
#35
JOURNAL ARTICLE
Seema Dua, Sonu Bansal, Devika Gautam, Bosco Jose, Priyanka Singh, Manoj Kumar Singh, Sachinandan De, Dharmendra Kumar, Prem Singh Yadav, Wilfried Kues, Naresh L Selokar
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and somatic cell nuclear transfer (SCNT) have been used to produce genome-edited farm animal species for improved production and health traits; however, these tools are rarely used in the buffalo and can play a pivotal role in milk and meat production in tropical and subtropical countries. In this study, we aimed to produce myostatin ( MSTN ) gene-edited embryos of the Murrah buffalo using the CRISPR/Cas9 system and SCNT. For this, fibroblast cells were electroporated with sgRNAs carrying all-in-one CRISPR/Cas9 plasmids targeting the first exon of the MSTN gene...
April 11, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37053510/the-emerging-biological-functions-of-exosomes-from-dental-tissue-derived-mesenchymal-stem-cells
#36
REVIEW
Shu Ma, Yidi Jiang, Yuyan Qian, Jing Du, Xiaoyan Yu, Shiyi Luo, Zhu Chen
Exosomes are one kind of small-cell extracellular membranous vesicles that can regulate intercellular communication and give rise to mediating the biological behaviors of cells, involving in tissue formation, repair, the modulation of inflammation, and nerve regeneration. The abundant kinds of cells can secret exosomes, among them, mesenchymal stem cells (MSCs) are very perfect cells for mass production of exosomes. Dental tissue-derived mesenchymal stem cells (DT-MSCs), including dental pulp stem cells, stem cells from exfoliated deciduous teeth, stem cells from apical papilla, stem cells from human periodontal ligament (PDLSCs), gingiva-derived mesenchymal stem cells, dental follicle stem cells, tooth germ stem cells, and alveolar bone-derived mesenchymal stem cells, are now known as a potent tool in the area of cell regeneration and therapy, more importantly, DT-MSCs can also release numerous types of exosomes, participating in the biological functions of cells...
April 2023: Cellular Reprogramming
https://read.qxmd.com/read/37053509/reprogramming-stars-11-teaming-up-to-uncover-the-epitranscriptomics-of-reprogramming-an-interview-with-dr-miguel-fidalgo-and-dr-diana-guallar
#37
JOURNAL ARTICLE
Miguel Fidalgo, Diana Guallar, Carlos-Filipe Pereira
No abstract text is available yet for this article.
April 2023: Cellular Reprogramming
https://read.qxmd.com/read/36939858/treatments-of-porcine-nuclear-recipient-oocytes-and-somatic-cell-nuclear-transfer-generated-embryos-with-various-reactive-oxygen-species-scavengers-lead-to-improvements-of-their-quality-parameters-and-developmental-competences-by-mitigating-oxidative-stress
#38
JOURNAL ARTICLE
Seung-Hwan Oh, Seung-Eun Lee, Dong-Hun Han, Jae-Wook Yoon, So-Hee Kim, Eun-Seo Lim, Han-Bi Lee, Eun-Young Kim, Se-Pill Park
This study investigated the antioxidant effects of β-cryptoxanthin (BCX), hesperetin (HES), and icariin (ICA), and their effects on in vitro maturation of porcine oocytes and subsequent embryonic development of somatic cell nuclear transfer (SCNT). Treatment with 1 μM BCX (BCX-1) increased the developmental rate of porcine oocytes more than treatment with 100 μM HES (HES-100) or 5 μM ICA (ICA-5). The glutathione level and mRNA expression of antioxidant genes ( NFE2L2 , SOD1 , and SOD2) were more increased in the BCX-1 group than in the HES-100 and ICA-5 groups, while the reactive oxygen species level was more decreased...
March 20, 2023: Cellular Reprogramming
https://read.qxmd.com/read/36927112/evaluation-of-all-human-transcription-factors-on-the-directed-differentiation-of-pluripotent-stem-cells
#39
Rodrigo L Dos Santos
No abstract text is available yet for this article.
March 16, 2023: Cellular Reprogramming
https://read.qxmd.com/read/36847738/a-fundamental-research-in-in-vitro-spermatogonial-stem-cell-culturing-what-are-clump-cells
#40
JOURNAL ARTICLE
Kiana Sojoudi, Hossein Azizi, Thomas Skutella
Spermatogonial stem cells (SSCs) are a small group of testicular cells located in the basement membrane of seminiferous tubules and can balance self-renewal and differentiation during spermatogenesis. Our in vitro culture experiments of mouse SSCs indicated heterogeneity of cultured cells. Highly compact colonies were observed next to SSC colonies, which we call clump cells. We used immunocytochemical staining to identify SSCs and somatic cells with VASA and Vimentin antibodies. Subsequently, we compared mRNA expression levels of VASA , DAZL , PLZF , GFRA1 , Lin28 , Kit , Myc and Vimentin genes using Fluidigm real-time RT-polymerase chain reaction in clump cells, SSCs, and testicular stromal cells...
February 27, 2023: Cellular Reprogramming
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