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Cellular Reprogramming

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https://www.readbyqxmd.com/read/29782192/the-histone-deacetylase-inhibitor-ci994-improves-nuclear-reprogramming-and-in-vitro-developmental-potential-of-cloned-pig-embryos
#1
Long Jin, Qing Guo, Guang-Lei Zhang, Xiao-Xu Xing, Mei-Fu Xuan, Qi-Rong Luo, Zhao-Bo Luo, Jun-Xia Wang, Xi-Jun Yin, Jin-Dan Kang
Epigenetic reprogramming and somatic cell nuclear transfer (SCNT) cloning efficiency were recently enhanced using histone deacetylase inhibitors (HDACis). In this study, we investigated the time effect of CI994, an HDACi, on the blastocyst formation rate, acetylation levels of H3K9 and H4K12, DNA methylation levels of anti-5-methylcytosine (5mC), and some mRNA expression of pluripotency-related genes in pig SCNT embryos. Treatment with 10 μM CI994 for 24 hours significantly improved the blastocyst formation rate of SCNT embryos in comparison with the untreated group (p < 0...
May 21, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29782191/lipid-protein-and-microrna-composition-within-mesenchymal-stem-cell-derived-exosomes
#2
Hao Deng, Chun Sun, Yingxin Sun, Huhu Li, Lin Yang, Danbin Wu, Qing Gao, Xijuan Jiang
Mesenchymal stem cells (MSCs) were regarded as one of the most promising type of seed cells in tissue engineering due to its easy accessibility and multipotent feature of being able to differentiate into adipocyte, osteoblast, cardiomyocytes, and neurons. For years, MSCs have been applied in treating cardiovascular disease, reconstructing kidney injury, and remodeling immune system with remarkable achievements. Basic researches revealed that its clinic effects are not only due to their pluripotent ability but also through their paracrine function that they synthesize and secrete a broad spectrum of growth factors and cytokines...
May 21, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29782184/the-growth-of-preantral-follicles-and-the-impact-of-different-supplementations-and-circumstances-a-review-study-with-focus-on-bovine-and-human-preantral-follicles
#3
Zahra Rajabi, Zunair Khokhar, Hossein Yazdekhasti
One of the most important concerns cancer survivors face is fertility. Current treatment modalities often result in damage to the reproductive system. Different options have been proposed to preserve the fertility of affected women, and many attempts have been made to improve their chance of childbearing after therapy. Cryopreservation of ovarian tissue and follicles before the onset of cancer treatment and then either transplantation of ovarian tissue or culture of ovarian tissue and individual follicles in vitro is a commonly cited approach...
May 21, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29708768/fibroblast-growth-factor-10-enhances-the-developmental-efficiency-of-somatic-cell-nuclear-transfer-embryos-by-accelerating-the-kinetics-of-cleavage-during-in-vitro-maturation
#4
Yeo-Jin Son, Seung-Eun Lee, Yun-Gwi Park, Sang-Gi Jeong, Min-Young Shin, Eun-Young Kim, Se-Pill Park
Somatic cell nuclear transfer (SCNT) is required for the generation of transgenic animals as disease models. During the in vitro development of SCNT embryos, the quality of matured oocytes is one of the major factors regulating the developmental potential of embryos. Time-lapse monitoring systems are new tools that assess the developmental capacity of embryos for use in embryo transfer. In this study, we investigated the effect of fibroblast growth factor 10 (FGF 10) on the developmental potential of SCNT embryos...
April 30, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29688743/serial-culture-is-critical-for-in-vitro-development-of-parthenogenetic-embryos-in-the-golden-syrian-hamster
#5
Guangbin Zhou, Hengxi Wei, Xinrong Wang, Min Yang, Thomas D Bunch, Irina A Polejaeva, Kenneth L White, Zhongde Wang, Qinggang Meng
Unlike oocytes of many other mammalian species, parthenogenetically activated hamster oocytes have not been reported to develop beyond the two-cell stage. This study investigated the in vitro development into blastocysts of parthenogenetic embryos of Golden Syrian hamsters. We observed that hamster oocytes could easily be artificially activated (AA) by treatment with ionomycin plus 6-dimethylaminopurine + cycloheximide + cytochalasin B as assessed by embryo cleavage in HECM-9 (63.15%) or HECM-10 (63.82%). None of the cleaved embryos developed beyond the two-cell stage when cultured in either of the two media...
April 24, 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29620445/induction-of-human-adipose-derived-mesenchymal-stem-cells-into-germ-lineage-using-retinoic-acid
#6
Haihui Liu, Mingtai Chen, Lulu Liu, Saisai Ren, Panpan Cheng, Hao Zhang
Accumulating evidence indicates that mesenchymal stem cells (MSCs) have been widely used in tissue engineering and regenerative medicine due to their multilineage differentiation potentials. Recent studies show that germ-like cells can also be derived from stem cells, such as human umbilical cord MSCs and human bone marrow MSCs in vitro. However, whether human adipose-derived MSCs (hAD-MSCs) can be induced into germ-like cells has never been reported. In this study, we isolated hAD-MSCs and confirmed that their characteristics were in accordance with that of MSCs established before...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29620444/cloning-of-buffalo-a-highly-valued-livestock-species-of-south-and-southeast-asia-any-achievements
#7
Naresh L Selokar, Monika Saini, Prabhat Palta, Manmohan S Chauhan, Radhey S Manik, Suresh K Singla
Buffalo (Bubalus bubalis) is a major source of milk, meat, and draught power in many developing countries in Asia. Animal cloning holds a lot of potential for fast multiplication of elite buffaloes and conservation of their valuable germplasm. Although the progress of buffalo cloning has been slow in comparison to cattle or pig, several breakthroughs were reported in buffalo cloning such as the production of cloned calves from somatic cells isolated from over one-decade old frozen-thawed semen or from urine-derived cells...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29620443/mir-2861-behaves-as-a-biomarker-of-lung-cancer-stem-cells-and-regulates-the-hdac5-erk-system-genes
#8
Mengya Zhao, Lin Li, Jundong Zhou, Xueyuan Cui, Qingmei Tian, Yaqing Jin, Yimin Zhu
Cancer stem cells (CSCs) are responsible for cancer initiating, recurrence, and drug resistance. Discovery of novel biomarkers for CSCs is helpful for early diagnosis and prognosis. Lung cancer stem cells (LCSCs) were closely related to the occurrence and development of lung cancer. In our study, the important role of miR-2861 in maintaining the stemness of LCSCs was investigated. The LCSC differentiation model was established through introducing serum into the medium of H460 spheres. miR-2861 expression was significantly higher in LCSCs no matter compared to the differentiation cells or normal cells...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29620442/mir-21-stat3-signal-is-involved-in-odontoblast-differentiation-of-human-dental-pulp-stem-cells-mediated-by-tnf-%C3%AE
#9
Ke Xu, Jingwen Xiao, Ke Zheng, Xingmei Feng, Jinlong Zhang, Donghui Song, Chenfei Wang, Xiang Shen, Xin Zhao, Changbo Wei, Dan Huang, Guijuan Feng
Dental pulp stem cells (DPSCs), as one type of mesenchymal stem cells (MSCs), have the capability of self-renewal and multipotency to differentiate into several cell lineages, including osteogenesis, odontoblasts, chondrogenesis, neurogenesis, and adipogenesis. It has found that tumor necrosis factor-α (TNF-α) can promote osteogenic differentiation of human DPSCs in our previous studies. Other experimentation revealed that signal transducer and activator of transcription 3 (STAT3) underwent a rapid activation both in osteogenesis and inflammation microenvironment of MSCs in vitro...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29620441/acknowledgment-of-reviewers-2017
#10
(no author information available yet)
No abstract text is available yet for this article.
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29486132/the-role-of-neuropilin-1-fyn-interaction-in-odontoblast-differentiation-of-dental-pulp-stem-cells
#11
Yihua Song, Peipei Cao, Zhifeng Gu, Jingwen Xiao, Min Lian, Dan Huang, Jing Xing, Ye Zhang, Xingmei Feng, Chenfei Wang
Abnormal odontoblast differentiation of dental pulp stem cells (DPSCs) caused by inflammation is closely related to the development of dental caries. Neuropilin-1 (NRP1) is one of the members of neuropilin family. It can combine with disparate ligands involved in regulating cell differentiation. FYN belongs to the protein-tyrosine kinase family, which has been implicated in the control of cell growth, and the effect can be further strengthened by inflammatory factors. In our studies, we verified that NRP1 can form complexes with FYN and have the correlation changes in odontoblast differentiation of DPSCs...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29446977/establishment-growth-proliferation-and-gene-expression-of-buffalo-bubalus-bubalis-transgenic-fetal-fibroblasts-containing-human-insulin-gene-and-production-of-embryos-by-handmade-cloning-using-these-cells
#12
Parul Mehta, Ramakant Kaushik, Karn Pratap Singh, Ankur Sharma, Manoj Kumar Singh, Manmohan Singh Chauhan, Prabhat Palta, Suresh Kumar Singla, Radhey Sham Manik
The aim of the present study was to compare transgenic cells, containing human insulin gene kept under the control of mammary gland-specific buffalo beta-lactoglobulin promoter, and their counterparts, that is, nontransgenic cells, for examining their potential for the production of embryos following somatic cell nuclear transfer (SCNT). The gene construct was delivered into buffalo fetal fibroblasts (BFF) by nucleofection following which, the transfected cells were selected by culture in the presence of G418 for 3 weeks...
April 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29412742/cloning-after-dolly
#13
Ian Wilmut, Jane Taylor
No abstract text is available yet for this article.
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29412741/pharmacologic-reprogramming-designed-to-induce-a-warburg-effect-in-porcine-fetal-fibroblasts-alters-gene-expression-and-quantities-of-metabolites-from-conditioned-media-without-increased-cell-proliferation
#14
Bethany R Mordhorst, Stephanie L Murphy, Rence M Ross, Melissa S Samuel, Shirley Rojas Salazar, Tieming Ji, Susanta K Behura, Kevin D Wells, Jonathan A Green, Randall S Prather
The Warburg effect is a metabolic phenomenon characterized by increased glycolytic activity, decreased mitochondrial oxidative phosphorylation, and the production of lactate. This metabolic phenotype is characterized in rapidly proliferative cell types such as cancerous cells and embryonic stem cells. We hypothesized that a Warburg-like metabolism could be achieved in other cell types by treatment with pharmacological agents, which might, in turn, facilitate nuclear reprogramming. The aim of this study was to treat fibroblasts with CPI-613 and PS48 to induce a Warburg-like metabolic state...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29412740/measles-virus-persistent-infection-of-human-induced-pluripotent-stem-cells
#15
Hila Naaman, Tatiana Rabinski, Avi Yizhak, Solly Mizrahi, Yonat Shemer Avni, Ran Taube, Bracha Rager, Yacov Weinstein, Glenn Rall, Jacob Gopas, Rivka Ofir
In this study, we found that the measles virus (MV) can infect human-induced pluripotent stem cells (hiPSCs). Wild-type MV strains generally use human signaling lymphocyte activation molecule (SLAM; CD150) as a cellular receptor, while vaccine strains such as the Edmonston strain can use both CD150 and CD46 as receptors. It is not yet known how early in the embryonal differentiation stages these receptors are expressed. We established two hiPSCs (BGU-iPSCs and EMF-iPSCs) which express CD46 and CD150. Both cell types can be infected by MV to form persistent, noncytopathic cell lines that release infectious MV particles...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29412739/mc1568-enhances-histone-acetylation-during-oocyte-meiosis-and-improves-development-of-somatic-cell-nuclear-transfer-embryos-in-pig
#16
Huili Wang, Wei Cui, Chunhua Meng, Jun Zhang, Yinxia Li, Yong Qian, Guangdong Xing, Dongmin Zhao, Shaoxian Cao
An increasing number of studies have revealed that histone deacetylase (HDAC) mediated histone deacetylation is important for mammalian oocyte development. However, nonselective HDAC inhibitors (HDACi) were applied in most studies; the precise functions of specific HDAC classes during meiosis are poorly defined. In this study, the class IIa-specific HDACi MC1568 was used to reveal a crucial role of class IIa HDACs in the regulation of histone deacetylation during porcine oocyte meiosis. Besides, the functions of HDACs and histone acetyltransferases in regulating the balance of histone acetylation/deacetylation were also confirmed during oocyte maturation...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29412738/reciprocity-of-action-of-increasing-oct4-and-repressing-p53-in-transdifferentiation-of-mouse-embryonic-fibroblasts-into-cardiac-myocytes
#17
Hongran Wang, Shuying Zhao, Michelle Barton, Todd Rosengart, Austin J Cooney
p53 is a barrier to somatic cell reprogramming. Deletion or transient suppression of p53 increases the efficiency of reprogramming of somatic cells into induced pluripotent stem cells. Whether p53 represents an obstacle to a similar process transdifferentiation of somatic cells is unknown. However, it is predicted that inhibition of p53 would promote transdifferentiation of fibroblasts into cardiomyocytes. In this study, the effect of p53 on the capacity of cardiogenic transdifferentiation is evaluated using p53 wild-type (p53+/+ ), p53 heterozygous mutant (p53+/- ), and p53 homozygous mutant (p53-/- ) mouse embryonic fibroblasts (MEFs)...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29412737/high-pregnancy-and-calving-rates-with-a-limited-number-of-transferred-handmade-cloned-bovine-embryos
#18
Jenin Victor Cortez, Gábor Vajta, Nilton Murga Valderrama, Gleni Segura Portocarrero, Jorge Maicelo Quintana
A major obstacle of widespread commercial application of bovine somatic cell nuclear transfer is the low overall efficiency, that is, healthy calf-late pregnancy per transferred embryo rate. In this study, we report a series of experiments with a limited number of embryos created with handmade cloning (HMC) and transferred without or after open pulled straw vitrification. Embryo reconstruction was performed by using in vitro matured oocytes and adult ear skin fibroblasts. In two experiments, a total of 53 D7 blastocysts were developed from 188 reconstructed embryos...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29412736/epigenetic-alteration-of-donor-cells-with-histone-deacetylase-inhibitor-m-carboxycinnamic-acid-bishydroxymide-improves-the-in-vitro-developmental-competence-of-buffalo-bubalus-bubalis-cloned-embryos
#19
Himanshu Agrawal, Naresh Lalaji Selokar, Monika Saini, Manoj Kumar Singh, Manmohan Singh Chauhan, Prabhat Palta, Suresh Kumar Singla, Radhey Sham Manik
Epigenetic reprogramming is an indispensable process during the course of mammalian development, but aberrant in cloned embryos. The aim of this study was to examine the effect of donor cell treatment with histone deacetylase (HDAC) inhibitor m-carboxycinnamic acid bishydroxymide (CBHA) on cloned embryo development and establish its optimal concentration. Different concentrations of CBHA (2.5, 5.0, 10.0, and 20.0 μM) were used to treat buffalo adult fibroblast cells for 24 hours and effect on cell proliferation, gene expression, and histone modifications was analyzed...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29412735/analysis-of-h19-igf2-methylation-status-in-the-sperm-of-cloned-goats-and-their-offspring
#20
Yongjie Wan, Mingtian Deng, Guomin Zhang, Caifang Ren, Zifei Liu, Feng Wang
The H19/insulin-like growth factor 2 (Igf2) gene plays vital roles during development, but their expression and methylation status have not been elucidated in male cloned animals. In this study, we investigated the methylation status of imprinting control region (ICR) and differentially methylated region (DMR) of the H19/Igf2 locus in the sperm and testes of naturally reproduced Saanen dairy goats (NG, control), transgenic cloned dairy goats (CG), and the first generation of mating the CGs with the female NGs (FG)...
February 2018: Cellular Reprogramming
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