journal
https://read.qxmd.com/read/38300527/induction-of-transient-morula-like-cells-in-mice-through-stat3-activation
#1
Celia Fernandez-Rial, Miguel Fidalgo
Developing in vitro cell models that faithfully replicate the molecular and functional traits of cells from the earliest stages of mammalian development presents a significant challenge. The strategic induction of signal transducer and activator of transcription 3 (STAT3) phosphorylation, coupled with carefully defined culture conditions, facilitates the efficient reprogramming of mouse pluripotent cells into a transient morula-like cell (MLC) state. The resulting MLCs closely mirror their in vivo counterparts, exhibiting not only molecular resemblance but also the ability to differentiate into both embryonic and extraembryonic lineages...
January 31, 2024: Cellular Reprogramming
https://read.qxmd.com/read/38261417/cloned-foal-born-from-postmortem-obtained-ear-sample-refrigerated-for-5-days-before-fibroblast-isolation-and-decontamination-of-the-infected-monolayer-culture
#2
JOURNAL ARTICLE
Jenin V Cortez, Kylie Hardwicke, Christopher G Grupen, Muren Herrid, Zoltan Machaty, Gábor Vajta
A 6-year-old mare, a valuable polo horse, died of complications following postcolic surgery. To preserve its genetics, ear skin samples were collected immediately after death and stored in an equine embryo transfer medium at 4°C for 5 days. After trypsin digestion, monolayer fibroblast cultures were established, but signs of massive bacterial infection were found in all of them. As an ultimate attempt for rescue, rigorously and repeatedly washed cells were individually cultured in all wells of four 96-well dishes...
January 23, 2024: Cellular Reprogramming
https://read.qxmd.com/read/38127804/acknowledgment-of-reviewers-2023
#3
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
December 22, 2023: Cellular Reprogramming
https://read.qxmd.com/read/38381405/gene-therapy-mediated-partial-reprogramming-extends-lifespan-and-reverses-age-related-changes-in-aged-mice
#4
JOURNAL ARTICLE
Carolina Cano Macip, Rokib Hasan, Victoria Hoznek, Jihyun Kim, Yuancheng Ryan Lu, Louis E Metzger, Saumil Sethna, Noah Davidsohn
Aging is a complex progression of changes best characterized as the chronic dysregulation of cellular processes leading to deteriorated tissue and organ function. Although aging cannot currently be prevented, its impact on life- and healthspan in the elderly can potentially be minimized by interventions that aim to return these cellular processes to optimal function. Recent studies have demonstrated that partial reprogramming using the Yamanaka factors (or a subset; OCT4 , SOX2, and KLF4; OSK) can reverse age-related changes in vitro and in vivo ...
February 2024: Cellular Reprogramming
https://read.qxmd.com/read/38381404/reprogramming-stars-14-fast-forwarding-cellular-reprogramming-an-interview-with-dr-mark-kotter
#5
JOURNAL ARTICLE
Mark R Kotter, Carlos-Filipe Pereira
No abstract text is available yet for this article.
February 2024: Cellular Reprogramming
https://read.qxmd.com/read/38381403/acknowledgment-of-reviewers-2023
#6
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
February 2024: Cellular Reprogramming
https://read.qxmd.com/read/38381402/partial-reprogramming-as-a-method-for-regenerating-neural-tissues-in-aged-organisms
#7
REVIEW
Ali Saber Sichani, Somayeh Khoddam, Shayan Shakeri, Zahra Tavakkoli, Arad Ranji Jafroodi, Reza Dabbaghipour, Mohsen Sisakht, Jafar Fallahi
Aging causes numerous age-related diseases, leading the human species to death. Nevertheless, rejuvenating strategies based on cell epigenetic modifications are a possible approach to counteract disease progression while getting old. Cell reprogramming of adult somatic cells toward pluripotency ought to be a promising tool for age-related diseases. However, researchers do not have control over this process as cells lose their fate, and cause potential cancerous cells or unexpected cell phenotypes. Direct and partial reprogramming were introduced in recent years with distinctive applications...
February 2024: Cellular Reprogramming
https://read.qxmd.com/read/38060815/sustained-vision-recovery-by-osk-gene-therapy-in-a-mouse-model-of-glaucoma
#8
JOURNAL ARTICLE
Margarete M Karg, Yuancheng Ryan Lu, Nasrin Refaian, James Cameron, Emma Hoffmann, Cindy Hoppe, Shintaro Shirahama, Madhura Shah, Drenushe Krasniqi, Anitha Krishnan, Maleeka Shrestha, Yinjie Guo, Jennifer M Cermak, Michel Walthier, Kasia Broniowska, Sharon Rosenzweig-Lipson, Meredith Gregory-Ksander, David A Sinclair, Bruce R Ksander
Glaucoma, a chronic neurodegenerative disease, is a leading cause of age-related blindness worldwide and characterized by the progressive loss of retinal ganglion cells (RGCs) and their axons. Previously, we developed a novel epigenetic rejuvenation therapy, based on the expression of the three transcription factors Oct4 , Sox2 , and Klf4 (OSK), which safely rejuvenates RGCs without altering cell identity in glaucomatous and old mice after 1 month of treatment. In the current year-long study, mice with continuous or cyclic OSK expression induced after glaucoma-induced vision damage had occurred were tracked for efficacy, duration, and safety...
December 7, 2023: Cellular Reprogramming
https://read.qxmd.com/read/38134212/gene-regulatory-networks-improving-inferences-with-transfer-learning
#9
Marcelo Tigre Moura
Deep transfer learning improves the inference of gene regulatory networks in human cells, reveals disease-associated genes, and identifies network-based druggable targets in human heart disease.
December 2023: Cellular Reprogramming
https://read.qxmd.com/read/38011697/low-intensity-nanosecond-pulsed-electric-field-accelerates-osteogenic-transformation-of-human-dermal-fibroblasts-by-enhancing-cell-pluripotency
#10
JOURNAL ARTICLE
Jingtian Lai, Zewei Wang, Haiying Zhou, Pengfei Li, Hui Lu, Tian Tu
Autologous human fibroblasts have the potential to differentiate into the osteogenic lineage under specific conditions and can be utilized for bone regeneration. However, their efficiency is currently unsatisfactory. Recently, low-intensity nanosecond pulsed electric field (nsPEF) stimulation has been demonstrated to enhance cell pluripotency by activating epigenetic regulatory pathways. In this study, human dermal fibroblasts were exposed to different intensities of nsPEF to assess whether these exposures resulted in changes in proliferation rate, calcium salt deposition, and expression of differentiation-related markers in different experimental groups...
November 27, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37971885/allogeneic-mesenchymal-stem-cells-after-in-vivo-transplantation-a-review
#11
REVIEW
Derek B Asserson
Autologous mesenchymal stem cells (MSCs) are ideal for tissue regeneration because of their ability to circumvent host rejection, but their procurement and processing present logistical and time-sensitive challenges. Allogeneic MSCs provide an alternative cell-based therapy capable of positively affecting all human organ systems, and can be readily available. Extensive research has been conducted in the treatment of autoimmune, degenerative, and inflammatory diseases with such stem cells, and has demonstrated predominantly safe outcomes with minimal complications...
November 16, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37847899/pioneer-of-cloning-and-inspirational-figure-for-cellular-reprogramming-scientists-sir-ian-wilmut-july-7-1944-september-10-2023
#12
JOURNAL ARTICLE
Carlos-Filipe Pereira
No abstract text is available yet for this article.
October 2023: Cellular Reprogramming
https://read.qxmd.com/read/37847898/microscopic-analysis-of-cell-fate-alteration-induced-by-cell-fusion
#13
JOURNAL ARTICLE
Taisei Kumazaki, Chinatsu Yonekawa, Tomomi Tsubouchi
In mammals, differentiated cells generally do not de-differentiate nor undergo cell fate alterations. However, they can be experimentally guided toward a different lineage. Cell fusion involving two different cell types has long been used to study this process, as this method induces cell fate alterations within hours to days in a subpopulation of fused cells, as evidenced by changes in gene-expression profiles. Despite the robustness of this system, its use has been restricted by low fusion rates and difficulty in eliminating unfused populations, thereby compromising resolution...
October 2023: Cellular Reprogramming
https://read.qxmd.com/read/37847897/reprogramming-cell-identity-past-lessons-challenges-and-future-directions
#14
JOURNAL ARTICLE
José C R Silva
Reprogramming is traditionally defined as the fate conversion of a cell to a stage of increased developmental potential. In its broader meaning, the reprogramming term is also applied to all forms of cell fate conversion that do not follow a developmental trajectory. Reprogramming is now a well-established field of research that gained rapid progress upon the advent of induced pluripotency. In this perspective, I reflect on the reprogramming lessons of the past, in the contributions to other fields of research and on the potential transformative future use of reprogrammed cells and of its cell derivatives...
October 2023: Cellular Reprogramming
https://read.qxmd.com/read/37782910/induced-pluripotent-stem-cell-derived-chimeric-antigen-receptor-t-cells-the-intersection-of-stem-cells-and-immunotherapy
#15
REVIEW
Mohammad Reza Lahimchi, Faezeh Maroufi, Amirhosein Maali
Chimeric antigen receptor (CAR) T cell therapy is a promising cell-based immunotherapy applicable to various cancers. High cost of production, immune rejection, heterogeneity of cell product, limited cell source, limited expandability, and relatively long production time have created the need to achieve a universal allogeneic CAR-T cell product for "off-the-shelf" application. Since the innovation of induced pluripotent stem cells (iPSCs) by Yamanaka et al., extensive efforts have been made to prepare an unlimited cell source for regenerative medicine, that is, immunotherapy...
October 2023: Cellular Reprogramming
https://read.qxmd.com/read/37769039/hepatitis-b-virus-x-protein-increases-cellular-oct3-4-and-myc-and-facilitates-cellular-reprogramming
#16
JOURNAL ARTICLE
Madhusudana Girija Sanal, Sarita Gupta, Rahul Saha, Nisha Vats, Shiv Kumar Sarin
Hepatitis B virus x (HBx) is a multifunctional protein coded by the Hepatitis B virus that is involved in various cellular processes such as proliferation, cell survival/apoptosis, and histone methylation. HBx was reported to be associated with liver "cancer stem cells." The stemness inducing properties of HBx could also facilitate the generation of pluripotent stem cells from somatic cells. It is well established that somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) using a cocktail of transcription factors called Yamanaka's factors (YFs) (OCT4, SOX2, KLF4, and MYC)...
September 26, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37738321/the-ethics-of-cellular-reprogramming
#17
REVIEW
Anna Smajdor, Adrian Villalba
Louise Brown's birth in 1978 heralded a new era not just in reproductive technology, but in the relationship between science, cells, and society. For the first time, human embryos could be created, selected, studied, manipulated, frozen, altered, or destroyed, outside the human body. But with this possibility came a plethora of ethical questions. Is it acceptable to destroy a human embryo for the purpose of research? Or to create an embryo with the specific purpose of destroying it for research? In an attempt to construct ethical and legal frameworks for the new era of cellular reprogramming, legislators and ethicists have tried to distinguish between different kinds of biological entity...
September 22, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37725013/sirt6-knockdown-in-buffalo-fetal-fibroblasts-exacerbates-premature-senescence-caused-by-dna-and-telomere-damage
#18
JOURNAL ARTICLE
Jingyuan Liang, Jiayu Cui, Juanru Cheng, Yu Pan, Ruimen Zhang, Sufang Yang, Lingxiu Zou
As a gene with antiaging functions, sirtuin6 ( SIRT6 ) belonging to the sirtuin family plays a vital role in DNA repair, telomerase function, and cellular senescence, as well as maintains epigenomic stability and promotes longevity. However, its role in cell senescence in large animals, such as buffaloes, remains unknown. Fibroblasts are commonly used for somatic reprogramming, and their physiological characteristics affect the efficiency of this process. We aimed to elucidate the role of SIRT6 in cellular senescence and proliferation and analyze its effect on the biological function of buffalo fibroblasts to help improve the efficiency of buffalo somatic cell reprogramming...
September 19, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37725012/proteomic-analysis-and-reprogramming-potential-of-the-porcine-intra-ooplasmic-nanovesicles
#19
JOURNAL ARTICLE
Islam M Saadeldin, Seonggyu Bang, Abdulkadir Y Maigoro, Sung Ho Yun, Seung Il Kim, Sanghoon Lee, Jongki Cho
Oocytes contain reprogramming machinery that can transform somatic cells into totipotent cells. In this study, we aimed to isolate and characterize nanovesicles from mature porcine oocytes and described them for the first time as "intra-ooplasmic vesicles (IOVs)". Isolated IOVs had an average diameter of 186.3 ± 10.8 nm. Proteomic analysis revealed 467 peptide reads, with the top 20 proteins related to reprogramming, antioxidative defense, cytoskeleton, heat shock proteins, and metabolism...
September 19, 2023: Cellular Reprogramming
https://read.qxmd.com/read/37725011/efficient-a%C3%A2-t-to-c%C3%A2-g-base-editing-via-adenine-transversion-editors
#20
Muhammad Arslan Mahmood
Generating A-to-C transversions to correct defective alleles or introduce novel alleles has posed significant challenges. However, two recent studies focusing on adenine transversions have achieved successful A-to-C transversions in mouse embryos and plant cell. These remarkable accomplishments notably broaden the range of base editing and their applications both in fundamental research and in therapeutics.
September 19, 2023: Cellular Reprogramming
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