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Stem Cell Research & Therapy

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https://www.readbyqxmd.com/read/29212557/preconditioning-of-murine-mesenchymal-stem-cells-synergistically-enhanced-immunomodulation-and-osteogenesis
#1
Tzuhua Lin, Jukka Pajarinen, Akira Nabeshima, Laura Lu, Karthik Nathan, Eemeli Jämsen, Zhenyu Yao, Stuart B Goodman
BACKGROUND: Mesenchymal stem cells (MSCs) are capable of immunomodulation and tissue regeneration, highlighting their potential translational application for treating inflammatory bone disorders. MSC-mediated immunomodulation is regulated by proinflammatory cytokines and pathogen-associated molecular patterns such as lipopolysaccharide (LPS). Previous studies showed that MSCs exposed to interferon gamma (IFN-γ) and the proinflammatory cytokine tumor necrosis factor alpha (TNF-α) synergistically suppressed T-cell activation...
December 6, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29208033/evaluating-the-oxysterol-combination-of-22-s-hydroxycholesterol-and-20-s-hydroxycholesterol-in-periodontal-regeneration-using-periodontal-ligament-stem-cells-and-alveolar-bone-healing-models
#2
Jin-Sun Lee, EunJi Kim, Seonggu Han, Kyung Lhi Kang, Jung Sun Heo
BACKGROUND: Oxysterols, oxygenated by-products of cholesterol biosynthesis, play roles in various physiological and pathological systems. However, the effects of oxysterols on periodontal regeneration are unknown. This study investigated the effects of the specific oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol (SS) on the regeneration of periodontal tissues using in-vitro periodontal ligament stem cells (PDLSCs) and in-vivo models of alveolar bone defect...
December 6, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29208029/tissue-source-determines-the-differentiation-potentials-of-mesenchymal-stem-cells-a-comparative-study-of-human-mesenchymal-stem-cells-from-bone-marrow-and-adipose-tissue
#3
Liangliang Xu, Yamei Liu, Yuxin Sun, Bin Wang, Yunpu Xiong, Weiping Lin, Qiushi Wei, Haibin Wang, Wei He, Bin Wang, Gang Li
BACKGROUND: Mesenchymal stem cells (MSCs) possess intrinsic regeneration capacity as part of the repair process in response to injury, such as fracture or other tissue injury. Bone marrow and adipose tissue are the major sources of MSCs. However, which cell type is more effective and suitable for cell therapy remains to be answered. The intrinsic molecular mechanism supporting the assertion has also been lacking. METHODS: Human bone marrow-derived MSCs (BMSCs) and adipose tissue-derived MSCs (ATSCs) were isolated from bone marrow and adipose tissue obtained after total hip arthroplasty...
December 6, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29202871/mesenchymal-stem-cell-derived-extracellular-vesicles-for-kidney-repair-current-status-and-looming-challenges
#4
REVIEW
Arash Aghajani Nargesi, Lilach O Lerman, Alfonso Eirin
Novel therapies are urgently needed to address the rising incidence and prevalence of acute kidney injury (AKI) and chronic kidney disease (CKD). Mesenchymal stem/stromal cells (MSCs) have shown promising results in experimental AKI and CKD, and have been used in the clinic for more than a decade with an excellent safety profile. The regenerative effects of MSCs do not rely on their differentiation and ability to replace damaged tissues, but are primarily mediated by the paracrine release of factors, including extracellular vesicles (EVs), composed of microvesicles and exosomes...
December 4, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29202812/celastrol-improves-self-renewal-and-differentiation-of-human-tendon-derived-stem-cells-by-suppressing-smad7-through-hypoxia
#5
Tianyi Wu, Shenghe Liu, Gen Wen, Jia Xu, Yaling Yu, Yimin Chai
BACKGROUND: We aimed to evaluate the potential enhancing effect of celastrol on the stemness of human tendon-derived stem cells (hTSCs) in vitro and the underlying molecular mechanisms. METHODS: The capability of hTSC self-renewal was assessed by cell proliferation and colony formation as determined with the CCK-8 kit. Adipogenesis, chondrogenesis, and osteogenesis were determined by Oil Red O, Alcian Blue, and Alizarin Red staining, respectively. The relative mRNA levels of Sox9, PPARγ, Runx2, Smad7, and HIF1α were determined by real-time polymerase chain reaction (PCR)...
December 4, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29191249/tracing-gfp-labeled-wjmscs-in-vivo-using-a-chronic-salpingitis-model-an-animal-experiment
#6
Zhe Li, Zhao Zhang, Wai-Kit Ming, Xin Chen, Xiao-Min Xiao
BACKGROUND: The present study was conducted to evaluate the distribution of Wharton's jelly-derived mesenchymal stem cells (WJMSCs) and their repairing function on the oviduct. METHODS: WJMSCs were transfected with the LV3-GFP-PURO lentivirus. Female New Zealand rabbits (n = 24) were divided randomly into control A and B groups and experimental C and D groups to establish inflammation models. Sterile saline solution or WJMSCs were injected into rabbits via ear veins and/or genital tract perfusion once weekly for 3 weeks...
December 1, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29183402/two-sides-of-the-same-coin-unraveling-subtle-differences-between-human-embryonic-and-induced-pluripotent-stem-cells-by-raman-spectroscopy
#7
Elvira Parrotta, Maria Teresa De Angelis, Stefania Scalise, Patrizio Candeloro, Gianluca Santamaria, Mariagrazia Paonessa, Maria Laura Coluccio, Gerardo Perozziello, Stefania De Vitis, Antonella Sgura, Elisa Coluzzi, Vincenzo Mollace, Enzo Mario Di Fabrizio, Giovanni Cuda
BACKGROUND: Human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells, hold enormous promise for many biomedical applications, such as regenerative medicine, drug testing, and disease modeling. Although induced pluripotent stem cells resemble embryonic stem cells both morphologically and functionally, the extent to which these cell lines are truly equivalent, from a molecular point of view, remains controversial. METHODS: Principal component analysis and K-means cluster analysis of collected Raman spectroscopy data were used for a comparative study of the biochemical fingerprint of human induced pluripotent stem cells and human embryonic stem cells...
November 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29179771/paracrine-effects-of-human-amniotic-epithelial-cells-protect-against-chemotherapy-induced-ovarian-damage
#8
Qiuwan Zhang, Shixia Bu, Junyan Sun, Minhua Xu, Xiaofen Yao, Kunyan He, Dongmei Lai
BACKGROUND: Human amniotic epithelial cells (hAECs) are attractive candidates for regenerative medical therapy, with the potential to replace deficient cells and improve functional recovery after injury. Previous studies have demonstrated that transplantation of hAECs effectively alleviate chemotherapy-induced ovarian damage via inhibiting granulose cells apoptosis in animal models of premature ovarian failure/insufficiency (POF/POI). However, the underlying molecular mechanism accounting for hAECs-mediated ovarian function recovery is not fully understood...
November 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29179738/laser-modified-titanium-surfaces-enhance-the-osteogenic-differentiation-of-human-mesenchymal-stem-cells
#9
Tatiana A B Bressel, Jana Dara Freires de Queiroz, Susana Margarida Gomes Moreira, Jéssyca T da Fonseca, Edson A Filho, Antônio Carlos Guastaldi, Silvia Regina Batistuzzo de Medeiros
BACKGROUND: Titanium surfaces have been modified by various approaches with the aim of improving the stimulation of osseointegration. Laser beam (Yb-YAG) treatment is a controllable and flexible approach to modifying surfaces. It creates a complex surface topography with micro and nano-scaled patterns, and an oxide layer that can improve the osseointegration of implants, increasing their usefulness as bone implant materials. METHODS: Laser beam irradiation at various fluences (132, 210, or 235 J/cm2) was used to treat commercially pure titanium discs to create complex surface topographies...
November 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29178928/microrna-133-overexpression-promotes-the-therapeutic-efficacy-of-mesenchymal-stem-cells-on-acute-myocardial-infarction
#10
Yueqiu Chen, Yunfeng Zhao, Weiqian Chen, Lincen Xie, Zhen-Ao Zhao, Junjie Yang, Yihuan Chen, Wei Lei, Zhenya Shen
BACKGROUND: Our study aim was to evaluate the therapeutic efficacy and mechanisms of miR-133-overexpressing mesenchymal stem cells (MSCs) on acute myocardial infarction. METHODS: Rat MSCs were isolated and purified by whole bone marrow adherent culturing. After transfection with the agomir or antagomir of miR-133, MSCs were collected for assay of cell vitality, apoptosis, and cell cycle progression. At the same time, exosomes were isolated from the supernatant to analyze the paracrine miR-133...
November 25, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29157299/the-similarities-between-smdcs-and-regdcs-in-alleviating-the-immune-injury-caused-by-transplantation-of-hepatocytes-differentiated-from-escs
#11
Cheng Zhang, Wenwei Liao, Bing Cai, Furong Liu, Qiong Ke, Xiaofeng Zhu, Xiaoshun He, Anbin Hu
BACKGROUND: This study aimed to investigate the tolerogenic mechanisms induced by semimature dendritic cells (smDCs) and regulatory dendritic cells (regDCs) after transplantation of hepatocytes differentiated from mouse embryonic stem cells (ESCs) and to confirm the low immunogenicity of hepatocytes differentiated from ESCs. METHODS: Green fluorescent protein-labeled ESCs collected from 129 mice were cultured to differentiate into hepatocytes. smDCs and regDCs were cultured in vitro...
November 21, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29157289/vitamin-c-plus-hydrogel-facilitates-bone-marrow-stromal-cell-mediated-endometrium-regeneration-in-rats
#12
Huan Yang, Su Wu, Ran Feng, Junjiu Huang, Lixiang Liu, Feng Liu, Yuqing Chen
BACKGROUND: Intrauterine adhesion (IUA) is a common uterine cavity disease which can be caused by mechanical damage that may eventually lead to infertility and pregnancy abnormalities. Since the effect of therapeutic drugs appears disappointing, cell therapy has emerged as an alternative choice for endometrium regeneration. The aim of this study is to investigate whether the combination of hydrogel Pluronic F-127 (PF-127), Vitamin C (Vc), and a bone marrow stromal cell (BMSC) mixture could be a feasible strategy to improve the endometrial regeneration in a mechanical damage model of IUA in rats...
November 21, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29145866/calcium-containing-scaffolds-induce-bone-regeneration-by-regulating-mesenchymal-stem-cell-differentiation-and-migration
#13
Rubén Aquino-Martínez, Alcira P Angelo, Francesc Ventura Pujol
BACKGROUND: Osteoinduction and subsequent bone formation rely on efficient mesenchymal stem cell (MSC) recruitment. It is also known that migration is induced by gradients of growth factors and cytokines. Degradation of Ca2+-containing biomaterials mimics the bone remodeling compartment producing a localized calcium-rich osteoinductive microenvironment. The aim of our study was to determine the effect of calcium sulfate (CaSO4) on MSC migration. In addition, to evaluate the influence of CaSO4 on MSC differentiation and the potential molecular mechanisms involved...
November 16, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29141687/autologous-adipose-derived-regenerative-cell-therapy-modulates-development-of-hypertrophic-scarring-in-a-red-duroc-porcine-model
#14
Philippe Foubert, Diana Zafra, Mike Liu, Rohit Rajoria, Damian Gutierrez, Mayer Tenenhaus, John K Fraser
BACKGROUND: Effective prevention and treatment of hypertrophic scars (HTSs), a common consequence of deep-partial thickness injury, remain a significant clinical challenge. Previous studies from our group have shown that autologous adipose-derived regenerative cells (ADRCs) represent a promising approach to improve wound healing and, thereby, impact HTS development. The purpose of this study was to assess the influence of local delivery of ADRCs immediately following deep-partial thickness cutaneous injury on HTS development in the red Duroc (RD) porcine model...
November 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29141683/pellet-coculture-of-osteoarthritic-chondrocytes-and-infrapatellar-fat-pad-derived-mesenchymal-stem-cells-with-chitosan-hyaluronic-acid-nanoparticles-promotes-chondrogenic-differentiation
#15
Shu Huang, Xiongbo Song, Tao Li, Jingfang Xiao, Yemiao Chen, Xiaoyuan Gong, Weinan Zeng, Liu Yang, Cheng Chen
BACKGROUND: Cell source plays a key role in cell-based cartilage repair and regeneration. Recent efforts in cell coculture have attempted to combine the advantages and negate the drawbacks of the constituent cell types. The aim of this study was to evaluate the chondrogenic outcome of articular chondrocytes (ACs) and infrapatellar fat pad (IPFP)-derived mesenchymal stem cells (MSCs) in direct coculture. METHODS: ACs and IPFP MSCs from the same patients with knee osteoarthritis (OA) were cocultured in monolayer and in pellets...
November 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29141662/safety-and-tolerability-of-intradiscal-implantation-of-combined-autologous-adipose-derived-mesenchymal-stem-cells-and-hyaluronic-acid-in-patients-with-chronic-discogenic-low-back-pain-1-year-follow-up-of-a-phase-i-study
#16
Hemant Kumar, Doo-Hoe Ha, Eun-Jong Lee, Jun Hee Park, Jeong Hyun Shim, Tae-Keun Ahn, Kyoung-Tae Kim, Alexander E Ropper, Seil Sohn, Chung-Hun Kim, Devang Kashyap Thakor, Soo-Hong Lee, In-Bo Han
BACKGROUND: Adipose tissue-derived mesenchymal stem cells (AT-MSCs) offer potential as a therapeutic option for chronic discogenic low back pain (LBP) because of their immunomodulatory functions and capacity for cartilage differentiation. The goal of this study was to assess the safety and tolerability of a single intradiscal implantation of combined AT-MSCs and hyaluronic acid (HA) derivative in patients with chronic discogenic LBP. METHODS: We performed a single-arm phase I clinical trial with a 12-month follow-up and enrolled 10 eligible chronic LBP patients...
November 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29141658/thioredoxin-mitigates-radiation-induced-hematopoietic-stem-cell-injury-in-mice
#17
Pasupathi Sundaramoorthy, Qinhong Wang, Zhihong Zheng, Yiqun Jiao, Benny J Chen, Phuong L Doan, Nelson J Chao, Yubin Kang
BACKGROUND: Radiation exposure poses a significant threat to public health. Hematopoietic injury is one of the major manifestations of acute radiation sickness. Protection and/or mitigation of hematopoietic stem cells (HSCs) from radiation injury is an important goal in the development of medical countermeasure agents (MCM). We recently identified thioredoxin (TXN) as a novel molecule that has marked protective and proliferative effects on HSCs. In the current study, we investigated the effectiveness of TXN in rescuing mice from a lethal dose of total body radiation (TBI) and in enhancing hematopoietic reconstitution following a lethal dose of irradiation...
November 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29132420/substance-p-and-patterned-silk-biomaterial-stimulate-periodontal-ligament-stem-cells-to-form-corneal-stroma-in-a-bioengineered-three-dimensional-model
#18
Jialin Chen, Wei Zhang, Peyman Kelk, Ludvig J Backman, Patrik Danielson
BACKGROUND: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP...
November 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29126441/tgf%C3%AE-3-recruits-endogenous-mesenchymal-stem-cells-to-initiate-bone-regeneration
#19
Moyuan Deng, Tieniu Mei, Tianyong Hou, Keyu Luo, Fei Luo, Aijun Yang, Bo Yu, Hao Pang, Shiwu Dong, Jianzhong Xu
BACKGROUND: The recruitment of a sufficient number of endogenous mesenchymal stem cells (MSCs) is the first stage of in-situ tissue regeneration. Transforming growth factor beta-3 (TGFβ3) could recruit stem or progenitor cells and endothelial cells to participate in tissue regeneration. However, the mechanism of TGFβ3 recruiting MSCs toward bone regeneration has remained obscure. METHODS: We estimated the promigratory property of TGFβ3 on human bone marrow MSCs (hBMSCs) cocultured with the vascular cells (human umbilical artery smooth muscle cells or human umbilical vein endothelial cells) or not by Transwell assay...
November 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29126438/therapeutic-effects-of-bone-marrow-derived-mononuclear-cells-from-healthy-or-silicotic-donors-on-recipient-silicosis-mice
#20
Helena D'Anunciação de Oliveira, Elga Bernardo Bandeira de Melo, Johnatas Dutra Silva, Jamil Zola Kitoko, Bianca Gutfilen, Thiago Barboza, Sergio Augusto Lopes de Souza, Christina Maeda Takiya, Patricia Rieken Macedo Rocco, Miquéias Lopes-Pacheco, Marcelo Marcos Morales
BACKGROUND: Administration of bone marrow mononuclear cells (BMMCs) modulates lung inflammation and fibrosis in experimental silicosis. However, no studies have evaluated whether silicosis affects the efficacy of autologous BMMCs treatment. We hypothesized that BMMCs obtained from healthy or silicotic mice may improve lung function, but they might affect the inflammatory and fibrotic processes differently in experimental silicosis. METHODS: C57BL/6 mice were randomly divided into control (C) and silicosis (SIL) groups...
November 10, 2017: Stem Cell Research & Therapy
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