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Stem Cell Research & Therapy

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https://www.readbyqxmd.com/read/29921325/inhibition-of-the-rhogtpase-cdc42-by-ml141-enhances-hepatocyte-differentiation-from-human-adipose-derived-mesenchymal-stem-cells-via-the-wnt5a-pi3k-mir-122-pathway-impact-of-the-age-of-the-donor
#1
Diana Chaker, Charbel Mouawad, Albert Azar, Didier Quilliot, Ibrahim Achkar, Ziad Fajloun, Nehman Makdissy
BACKGROUND: Human adipose-derived mesenchymal stem cells (hADSCs) are promising cells that may promote hepatocyte differentiation (Hep-Dif) and improve liver function, but the involvement of Cdc42, a key small RhoGTPase which plays a crucial role in aging, is still not well established. We hypothesized that the inhibition of Cdc42 may rescue the hepatogenic potential of hADSCs derived from aged donors. METHODS: hADSCs isolated from 61 women of different ages were cultured for evaluation of the proliferation of cells, adherence, apoptosis, immunomodulation, immunophenotyping, multipotency, gene expression, and cell function during Hep-Dif...
June 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29921317/bone-marrow-mesenchymal-stem-cells-do-not-enhance-intra-synovial-tendon-healing-despite-engraftment-and-homing-to-niches-within-the-synovium
#2
Mohammad R Khan, Jayesh Dudhia, Frederic H David, Roberta De Godoy, Vedika Mehra, Gillian Hughes, Stephanie G Dakin, Andrew J Carr, Allen E Goodship, Roger K W Smith
BACKGROUND: Intra-synovial tendon injuries display poor healing, which often results in reduced functionality and pain. A lack of effective therapeutic options has led to experimental approaches to augment natural tendon repair with autologous mesenchymal stem cells (MSCs) although the effects of the intra-synovial environment on the distribution, engraftment and functionality of implanted MSCs is not known. This study utilised a novel sheep model which, although in an anatomically different location, more accurately mimics the mechanical and synovial environment of the human rotator cuff, to determine the effects of intra-synovial implantation of MSCs...
June 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29921316/induced-pluripotent-stem-cell-derived-mesenchymal-stem-cells-activate-quiescent-t-cells-and-elevate-regulatory-t-cell-response-via-nf-%C3%AE%C2%BAb-in-allergic-rhinitis-patients
#3
Xing-Liang Fan, Qing-Xiang Zeng, Xin Li, Cheng-Lin Li, Zhi-Bin Xu, Xue-Quan Deng, Jianbo Shi, Dong Chen, Song Guo Zheng, Qing-Ling Fu
BACKGROUND: It has been demonstrated previously that induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (MSCs) have immunosuppressive effects on activated T cells. However, the effects of iPSC-MSCs on quiescent T cells are still unknown. The aim of this study was to identify the immunomodulatory role of iPSC-MSCs on resting peripheral blood mononuclear cells (PBMCs) from allergic rhinitis (AR) patients. METHODS: PBMCs were cocultured with iPSC-MSCs without any stimulation, following which lymphocyte proliferation, activation of T cells, TH 1/TH 2 and regulatory T (Treg) cell differentiation, and Treg cell function were analyzed...
June 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29921311/adipose-derived-and-bone-marrow-mesenchymal-stem-cells-a-donor-matched-comparison
#4
Samih Mohamed-Ahmed, Inge Fristad, Stein Atle Lie, Salwa Suliman, Kamal Mustafa, Hallvard Vindenes, Shaza B Idris
BACKGROUND: Adipose-derived stem cells (ASCs) have been introduced as an alternative to bone marrow mesenchymal stem cells (BMSCs) for cell-based therapy. However, different studies comparing ASCs and BMSCs have shown conflicting results. In fact, harvesting ASCs and BMSCs from different individuals might influence the results, making comparison difficult. Therefore, this study aimed to characterize donor-matched ASCs and BMSCs in order to investigate proliferation, differentiation potential and possible effects of donor variation on these mesenchymal stem cells (MSCs)...
June 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29921287/deer-thymosin-beta-10-functions-as-a-novel-factor-for-angiogenesis-and-chondrogenesis-during-antler-growth-and-regeneration
#5
Wei Zhang, Wenhui Chu, Qingxiu Liu, Dawn Coates, Yudong Shang, Chunyi Li
BACKGROUND: Deer antlers are the only known mammalian organ with vascularized cartilage that can completely regenerate. Antlers are of real significance as a model of mammalian stem cell-based regeneration with particular relevance to the fields of chondrogenesis, angiogenesis, and regenerative medicine. Recent research found that thymosin beta 10 (TMSB10) is highly expressed in the growth centers of growing antlers. The present study reports here the expression, functions, and molecular interactions of deer TMSB10...
June 19, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29914569/clonal-dynamics-studied-in-cultured-induced-pluripotent-stem-cells-reveal-major-growth-imbalances-within-a-few-weeks
#6
David Brenière-Letuffe, Aya Domke-Shibamiya, Arne Hansen, Thomas Eschenhagen, Boris Fehse, Kristoffer Riecken, Justus Stenzig
BACKGROUND: Human induced pluripotent stem (iPS) cells have revolutionised research and spark hopes for future tissue replacement therapies. To obtain high cell numbers, iPS cells can be expanded indefinitely. However, as long-term expansion can compromise cell integrity and quality, we set out to assess potential reduction of clonal diversity by inherent growth imbalances. METHODS: Using red, green, blue marking as a lentiviral multi-colour clonal cell tracking technology, we marked three different iPS cell lines as well as three other cell lines, assigning a unique fluorescent colour to each cell at one point in culture...
June 18, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29907164/optimizing-the-method-for-generation-of-integration-free-induced-pluripotent-stem-cells-from-human-peripheral-blood
#7
Haihui Gu, Xia Huang, Jing Xu, Lili Song, Shuping Liu, Xiao-Bing Zhang, Weiping Yuan, Yanxin Li
BACKGROUND: Generation of induced pluripotent stem cells (iPSCs) from human peripheral blood provides a convenient and low-invasive way to obtain patient-specific iPSCs. The episomal vector is one of the best approaches for reprogramming somatic cells to pluripotent status because of its simplicity and affordability. However, the efficiency of episomal vector reprogramming of adult peripheral blood cells is relatively low compared with cord blood and bone marrow cells. METHODS: In the present study, integration-free human iPSCs derived from peripheral blood were established via episomal technology...
June 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29907129/a-pivotal-role-of-bex1-in-liver-progenitor-cell-expansion-in-mice
#8
Yuting Gu, Weiting Wei, Yiji Cheng, Bing Wan, Xinyuan Ding, Hui Wang, Yanyun Zhang, Min Jin
BACKGROUND: The activation and expansion of bipotent liver progenitor cells (LPCs) are indispensable for liver regeneration after severe or chronic liver injury. However, the underlying molecular mechanisms regulating LPCs and LPC-mediated liver regeneration remain elusive. METHODS: Hepatic brain-expressed X-linked 1 (BEX1) expression was evaluated using microarray screening, real-time polymerase chain reaction, immunoblotting and immunofluorescence. LPC activation and liver injury were studied following a choline-deficient, ethionine-supplemented (CDE) diet in wild-type (WT) and Bex1-/- mice...
June 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29898789/co-stimulation-of-lpar-1-and-s1pr-1-3-increases-the-transplantation-efficacy-of-human-mesenchymal-stem-cells-in-drug-induced-and-alcoholic-liver-diseases
#9
Mianhuan Li, Yi Lv, Feng Chen, Xiaoyan Wang, Jiang Zhu, Hao Li, Jia Xiao
BACKGROUND: One of the major obstacles facing stem cell therapy is the limited number of functional stem cells available after transplantation due to the harsh microenvironment surrounding the damaged tissue. The aim of this study was to delineate the mechanistic involvement of lysophosphatidic acid receptors (LPARs) and sphingosine-1-phosphate receptors (S1PRs) in the regulation of anti-stress and transplantation efficacy of stem cells. METHODS: Human adipose-derived mesenchymal stem cells (hADMSCs) were treated with chemical toxin or ethanol to induce cell stress...
June 14, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29898779/melatonin-rescued-interleukin-1%C3%AE-impaired-chondrogenesis-of-human-mesenchymal-stem-cells
#10
Bo Gao, Wenjie Gao, Zizhao Wu, Taifeng Zhou, Xianjian Qiu, Xudong Wang, Chengjie Lian, Yan Peng, Anjing Liang, Jincheng Qiu, Yuanxin Zhu, Caixia Xu, Yibing Li, Peiqiang Su, Dongsheng Huang
BACKGROUND: Osteoarthritis (OA) is a widespread arthritic disease and a primary cause of disability. Increasing evidence suggests that inflammation has a pivotal part in its pathogenesis. Interleukin-1β (IL-1β) is a primary mediator of local inflammatory processes in OA. Current therapies for OA mainly focus on the symptoms of the advanced stage of the disease. The possible utilization of bone marrow mesenchymal stem cells (BMSCs) to regenerate cartilage is an appealing method, but in the case of OA requires chondrogenesis to take place within an inflamed environment...
June 14, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29895334/ex-vivo-evaluation-of-intravitreal-mesenchymal-stromal-cell-viability-using-bioluminescence-imaging
#11
Carolina Assis P Vilela, Lucas Eduardo B Souza, Rubens C Siqueira, Rodrigo T Calado, Dimas T Covas, Jayter S Paula
BACKGROUND: Bone marrow-derived mesenchymal stromal cell (MSC) therapy is a promising treatment for several degenerative ocular diseases; however, no reproducible method of monitoring these cells into the eye has been established. The aim of this study was to describe successful bioluminescence imaging (BLI) to detect viable luciferase-expressing MSC in the eye. METHODS: Human donor MSC in culture were transduced with 50 μl luciferase lentiviral vector (three viral particles/cell) prior to intraocular injection...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29895333/exosomes-secreted-by-adipose-derived-mesenchymal-stem-cells-regulate-type-i-collagen-metabolism-in-fibroblasts-from-women-with-stress-urinary-incontinence
#12
Xiaochun Liu, Shiwei Wang, Suhui Wu, Qian Hao, Ying Li, Zhuodan Guo, Wenzhen Wang
BACKGROUND: Mesenchymal stem cells (MSC) have gained credibility as a therapeutic tool partly due to their potential to secrete factors such as cytokines and chemokines. Recently, exosomes, which mediate intercellular communication by delivering biomolecules such as mRNA and miRNA into recipient cells, have gained attention as a new and valuable therapeutic strategy in regenerative medicine. However, the potential role of exosomes secreted by adipose-derived mesenchymal stem cells (adMSC-Exos) in collagen metabolism is not well understood...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29895327/conditioned-medium-from-bone-marrow-derived-mesenchymal-stem-cells-inhibits-vascular-calcification-through-blockade-of-the-bmp2-smad1-5-8-signaling-pathway
#13
Shuangshuang Wang, Siwang Hu, Jian Wang, Yahui Liu, Ruochi Zhao, Maoqing Tong, Hanbin Cui, Nan Wu, Xiaomin Chen
BACKGROUND: Arterial calcification is associated with cardiovascular disease as a complication of advanced atherosclerosis and is a significant contributor to cardiovascular morbidity and mortality. Osteoblastic differentiation of vascular smooth muscle cells (VSMCs) plays an important role in arterial calcification and is characterized by cellular necrosis, inflammation, and lipoprotein and phospholipid complexes, especially in atherosclerotic calcification. The conditioned medium from bone marrow-derived mesenchymal stem cells (MSC-CM) is well known as a rich source of autologous cytokines and is universally used for tissue regeneration in current clinical medicine...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29895321/neural-stem-cell-therapy-for-subacute-and-chronic-ischemic-stroke
#14
REVIEW
Austin C Boese, Quan-Son Eric Le, Dylan Pham, Milton H Hamblin, Jean-Pyo Lee
Neural stem cells (NSCs) play vital roles in brain homeostasis and exhibit a broad repertoire of potentially therapeutic actions following neurovascular injury. One such injury is stroke, a worldwide leading cause of death and disability. Clinically, extensive injury from ischemic stroke results from ischemia-reperfusion (IR), which is accompanied by inflammation, blood-brain barrier (BBB) damage, neural cell death, and extensive tissue loss. Tissue plasminogen activator (tPA) is still the only US Food and Drug Administration-approved clot-lysing agent...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29895317/hypoxia-with-wharton-s-jelly-mesenchymal-stem-cell-coculture-maintains-stemness-of-umbilical-cord-blood-derived-cd34-cells
#15
Dewan Zhao, Lingjia Liu, Qiang Chen, Fangfang Wang, Qiuyang Li, Qiang Zeng, Jingcao Huang, Maowen Luo, Wenxian Li, Yuhuan Zheng, Ting Liu
BACKGROUND: The physiological approach suggests that an environment associating mesenchymal stromal cells with low O2 concentration would be most favorable for the maintenance of hematopoietic stem/progenitor cells (HSPCs). To test this hypothesis, we performed a coculture of cord blood CD34+ cells with Wharton's jelly mesenchymal stem cells (WJ-MSCs) under different O2 concentration to simulate the growth of HSPCs in vivo, and assessed the impacts on stemness maintenance and proliferation of cord blood HSPCs in vitro...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29895313/use-of-bioreactors-for-culturing-human-retinal-organoids-improves-photoreceptor-yields
#16
Patrick Ovando-Roche, Emma L West, Matthew J Branch, Robert D Sampson, Milan Fernando, Peter Munro, Anastasios Georgiadis, Matteo Rizzi, Magdalena Kloc, Arifa Naeem, Joana Ribeiro, Alexander J Smith, Anai Gonzalez-Cordero, Robin R Ali
BACKGROUND: The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies and disease modelling relies on the ability to obtain healthy and organised retinal tissue in sufficient quantities. Generating such tissue is a lengthy process, often taking over 6 months of cell culture, and current approaches do not always generate large quantities of the major retinal cell types required. METHODS: We adapted our previously described differentiation protocol to investigate the use of stirred-tank bioreactors...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29895312/mesenchymal-stem-cells-and-porous-%C3%AE-tricalcium-phosphate-composites-prepared-through-stem-cell-screen-enrich-combine-biomaterials-circulating-system-for-the-repair-of-critical-size-bone-defects-in-goat-tibia
#17
Wenxiang Chu, Yaokai Gan, Yifu Zhuang, Xin Wang, Jie Zhao, Tingting Tang, Kerong Dai
BACKGROUND: Efficacious bone substitute is essential for the treatment of a critical size bone defect. Currently, the bone substitutes commonly used in clinical practice lack osteogenic capacity and the therapeutic efficacy is not ideal. Herein, a novel stem cell screen-enrich-combine(-biomaterials) circulating system (SECCS) was introduced to provide the substitutes with osteogenic ability. The stem cell screening, enrichment, and recombination with substitutes could be integrated during the surgical operation...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29895311/epithelial-disruption-a-new-paradigm-enabling-human-airway-stem-cell-transplantation
#18
Nigel Farrow, Patricia Cmielewski, Martin Donnelley, Nathan Rout-Pitt, Yuben Moodley, Ivan Bertoncello, David Parsons
BACKGROUND: Airway disease is a primary cause of morbidity and early mortality for patients with cystic fibrosis (CF). Cell transplantation therapy has proven successful for treating immune disorders and may have the potential to correct the airway disease phenotype associated with CF. Since in vivo cell delivery into unconditioned mouse airways leads to inefficient engraftment, we hypothesised that disrupting the epithelial cell layer using the agent polidocanol (PDOC) would facilitate effective transplantation of cultured stem cells in mouse nasal airways...
June 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29871694/safety-and-efficacy-of-human-embryonic-stem-cell-derived-astrocytes-following-intrathecal-transplantation-in-sod1-g93a-and-nsg-animal-models
#19
Michal Izrael, Shalom Guy Slutsky, Tamar Admoni, Louisa Cohen, Avital Granit, Arik Hasson, Joseph Itskovitz-Eldor, Lena Krush Paker, Graciela Kuperstein, Neta Lavon, Shiran Yehezkel Ionescu, Leonardo Javier Solmesky, Rachel Zaguri, Alina Zhuravlev, Ella Volman, Judith Chebath, Michel Revel
BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a motor neuron (MN) disease characterized by the loss of MNs in the central nervous system. As MNs die, patients progressively lose their ability to control voluntary movements, become paralyzed and eventually die from respiratory/deglutition failure. Despite the selective MN death in ALS, there is growing evidence that malfunctional astrocytes play a crucial role in disease progression. Thus, transplantation of healthy astrocytes may compensate for the diseased astrocytes...
June 6, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29848383/mir-10a-rejuvenates-aged-human-mesenchymal-stem-cells-and-improves-heart-function-after-myocardial-infarction-through-klf4
#20
Jun Dong, Zhenhui Zhang, Hongshen Huang, Pei Mo, Chuanfan Cheng, Jianwei Liu, Weizhao Huang, Chaowei Tian, Chongyu Zhang, Jiao Li
BACKGROUND: Aging is one of the key factors that regulate the function of human bone marrow mesenchymal stem cells (hBM-MSCs) and related changes in microRNA (miRNA) expression. However, data reported on aging-related miRNA changes in hBM-MSCs are limited. METHODS: We demonstrated previously that miR-10a is significantly decreased in aged hBM-MSCs and restoration of the miR-10a level attenuated cell senescence and increased the differentiation capacity of aged hBM-MSCs by repressing Krüpple-like factor 4 (KLF4)...
May 30, 2018: Stem Cell Research & Therapy
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