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Stem Cell Research & Therapy

Rosanna C Ching, Mikael Wiberg, Paul J Kingham
BACKGROUND: Adipose derived stem cells can be stimulated to produce a growth factor rich secretome which enhances axon regeneration. In this study we investigated the importance of exosomes, extracellular vesicles released by many different cell types, including stem cells and endogenous nervous system Schwann cells (SCs), on neurite outgrowth. METHODS: Adipose derived stem cells were differentiated towards a Schwann cell-like phenotype (dADSCs) by in vitro stimulation with a mix of factors (basic fibroblast growth factor, platelet derived growth factor-AA, neuregulin-1 and forskolin)...
October 11, 2018: Stem Cell Research & Therapy
Ben Antebi, Luis A Rodriguez, Kerfoot P Walker, Amber M Asher, Robin M Kamucheka, Lucero Alvarado, Arezoo Mohammadipoor, Leopoldo C Cancio
BACKGROUND: In the bone marrow, MSCs reside in a hypoxic milieu (1-5% O2 ) that is thought to preserve their multipotent state. Typically, in vitro expansion of MSCs is performed under normoxia (~ 21% O2 ), a process that has been shown to impair their function. Here, we evaluated the characteristics and function of MSCs cultured under hypoxia and hypothesized that, when compared to normoxia, dedicated hypoxia will augment the functional characteristics of MSCs. METHODS: Human and porcine bone marrow MSCs were obtained from fresh mononuclear cells...
October 11, 2018: Stem Cell Research & Therapy
Severin Mühleder, Karoline Pill, Mira Schaupper, Krystyna Labuda, Eleni Priglinger, Pablo Hofbauer, Verena Charwat, Uwe Marx, Heinz Redl, Wolfgang Holnthoner
The original article [1] contains numerous value errors in the graphs in Fig. 2b regarding the markers describing the values for total tubule length and mean tubule length without aprotinin at 2.5 mg/ml concentration of fibrinogen. The corrected version of this figure can be viewed ahead.
October 7, 2018: Stem Cell Research & Therapy
Bushra Memon, Manale Karam, Sara Al-Khawaga, Essam M Abdelalim
The original article [1] contains a number of small errors which the authors would like to clarify.
October 7, 2018: Stem Cell Research & Therapy
Fa-Ming Chen, Li-Na Gao, Bei-Min Tian, Xi-Yu Zhang, Yong-Jie Zhang, Guang-Ying Dong, Hong Lu, Qing Chu, Jie Xu, Yang Yu, Rui-Xin Wu, Yuan Yin, Songtao Shi, Yan Jin
The original article [1] contains a major error carried across the captions of Tables 1, 2, and 3. In each table caption, the data were expressed as "mean ± standard deviation (SD)"; unfortunately, the authors had mistakenly expressed the data as "mean ± standard error (SE)" instead. As such, all mentions of "mean ± standard error" in those table captions should of course state "mean ± standard deviation". The authors are deeply sorry for these errors.
October 7, 2018: Stem Cell Research & Therapy
Vitor Hugo Santos, João Pedro Hübbe Pfeifer, Jaqueline Brandão de Souza, Betsabéia Heloisa Gentilha Milani, Rogério Antonio de Oliveira, Marjorie Golim Assis, Elenice Deffune, Andrei Moroz, Ana Liz Garcia Alves
The original article [1] contained a minor error regarding the mean diameter of the alginate microcapsules described in relation to Fig. 4 in the Results section. The microcapsules had an actual mean diameter of 3000 μm instead of 1000 μm as mistakenly mentioned in the original article.
October 7, 2018: Stem Cell Research & Therapy
Yantao He, Dongmei Chen, Lingling Yang, Qiaoni Hou, Huiming Ma, Xian Xu
With the development of regenerative medicine, a variety of mesenchymal stem cells (MSCs) are increasingly considered for the treatment of premature ovarian failure (POF). Reportedly, bone marrow-derived MSCs (BMSCs) improve the ovarian reserve, which mainly depends on homing and paracrine activities. Furthermore, paracrine factors secreted by these stem cells play an important role in ovarian recovery. Relevant studies indicate that BMSC transplantation has some positive effects on the treatment of POF in animals, but BMSCs are not widely applied in clinical therapy...
October 4, 2018: Stem Cell Research & Therapy
Sumanta Chatterjee, Pratima Basak, Edward Buchel, Leigh C Murphy, Afshin Raouf
BACKGROUND: Normal human breast epithelial cells are maintained by the proliferation and differentiation of different human breast epithelial progenitors (HBEPs). However, these progenitor subsets can only be obtained at low frequencies, limiting their further characterization. Recently, it was reported that HBEPs can be minimally expanded in Matrigel cocultures with stromal feeder cells. However, variability of generating healthy feeder cells significantly impacts the effective expansion of HBEPs...
October 4, 2018: Stem Cell Research & Therapy
Kamil Kowalski, Matthieu Dos Santos, Pascal Maire, Maria A Ciemerych, Edyta Brzoska
BACKGROUND: Skeletal muscle regeneration is possible thanks to unipotent stem cells, which are satellite cells connected to the myofibers. Populations of stem cells other than muscle-specific satellite cells are considered as sources of cells able to support skeletal muscle reconstruction. Among these are bone marrow-derived mesenchymal stem cells (BM-MSCs), which are multipotent, self-renewing stem cells present in the bone marrow stroma. Available data documenting the ability of BM-MSCs to undergo myogenic differentiation are not definitive...
September 27, 2018: Stem Cell Research & Therapy
Yang Yu, Qingyun Zhang, Chengzhong Ma, Xue Yang, Rui Lin, Hongxiang Zhang, Yan Liu, Zhipeng Han, Jiwen Cheng
BACKGROUND: Androgen deprivation (AD) as the first-line treatment for advanced prostate cancer (PCa) is insufficient for a long-term effect. Castration resistance remains the greatest obstacle in PCa clinical therapy. Mesenchymal stem cells (MSCs) can migrate into PCa tissues contributing to tumor progression, therefore, in this study we explored the effect of AD on MSC migration to PCa and elicited its importance for the emergence of castration resistance. METHODS: MSC migration assay was performed in several PCa cells (LNCaP, VCaP, and 22Rv1) using in-vivo and in-vitro approaches...
September 26, 2018: Stem Cell Research & Therapy
Nayra Cárdenes, Diana Álvarez, Jacobo Sellarés, Yating Peng, Catherine Corey, Sophie Wecht, Seyed Mehdi Nouraie, Swaroop Shanker, John Sembrat, Marta Bueno, Sruti Shiva, Ana L Mora, Mauricio Rojas
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease for which age is the most important risk factor. Different mechanisms associated with aging, including stem cell dysfunction, have been described to participate in the pathophysiology of IPF. We observed an extrapulmonary effect associated with IPF: increase in cell senescence of bone marrow-derived mesenchymal stem cells (B-MSCs). METHODS: B-MSCs were obtained from vertebral bodies procured from IPF patients and age-matched normal controls...
September 26, 2018: Stem Cell Research & Therapy
Syed Shadab Raza, Aurel Popa Wagner, Yawer S Hussain, Mohsin Ali Khan
BACKGROUND: Neurodegenerative disorders have a complex pathology and are characterized by a progressive loss of neuronal architecture in the brain or spinal cord. Neuroprotective agents have demonstrated promising results at the preclinical stage, but this has not been confirmed at the clinical stage. Thus far, no neuroprotective drug that can prevent neuronal degeneration in patients with neurodegenerative disorders is available. MAIN BODY: Recent studies have focused on neurorestorative measures, such as cell-based therapy, rather than neuroprotective treatment...
September 26, 2018: Stem Cell Research & Therapy
Keykavos Gholami, Gholamreza Pourmand, Morteza Koruji, Sepideh Ashouri, Mehdi Abbasi
BACKGROUND: In-vitro spermatogenesis in mammalian species is considered an important topic in reproductive biology. New strategies for achieving a complete version of spermatogenesis ex vivo have been conducted using an organ culture method or culture of testicular cells in a three-dimensional soft agar culture system (SACS). The aim of this study was to develop a new method that supports spermatogenesis to the meiotic phase and morphologically mature spermatozoa through the culture of testicular cells and seminiferous tubules (STs) in a modified SACS, respectively...
September 26, 2018: Stem Cell Research & Therapy
Luis Barbeito
Amyotrophic lateral sclerosis (ALS) is a fatal paralytic disease with no cure or treatment to stop disease progression. Because ALS represents an urgent unmet medical need, a significant number of therapeutics are being tested in preclinical and clinical studies. A recent publication in Stem Cell Research & Therapy by Izrael and colleagues reports about embryonic stem cell-derived astrocytes as a potential cell therapy for ALS. Such cells behave as highly trophic "young astrocytes", being able to delay disease onset and prolong survival when injected intrathechally in murine models of ALS overexpressing the SOD1G93A mutation...
September 26, 2018: Stem Cell Research & Therapy
Hao Guo, Baozhu Li, Wei Wang, Na Zhao, Haopeng Gao
Inducing donor-specific immunological tolerance, which avoids the complications of long-term immunosuppression, is an important goal in organ transplantation. Interleukin-35 (IL-35), a cytokine identified in 2007, is mainly secreted by regulatory T cells (Tregs) and is essential for Tregs to exert their maximal immunoregulatory activity in vitro and in vivo. A growing number of studies show that IL-35 plays an important role in autoimmune diseases and infectious diseases. Recent research has shown that IL-35 could effectively alleviate allograft rejection and has the potential to be a novel therapeutic strategy for graft rejection...
September 26, 2018: Stem Cell Research & Therapy
Xi Ouyang, Xiaoyan Han, Zehong Chen, Jiafeng Fang, Xuna Huang, Hongbo Wei
BACKGROUND: This study investigated the therapeutic effects of MSC-derived exosomes (MSC-Exos) on erectile function in a rat model of cavernous nerve injury (CNI). METHODS: MSCs were isolated from rat bone marrow and exosomes were isolated from the supernatants by ultracentrifugation. The tissue explant adherent method was used to isolate and culture corpus cavernosum smooth muscle cells (CCSMCs). MSCs and CCSMCs were identified by flow cytometry, in vitro differentiation or immunofluorescence staining...
September 26, 2018: Stem Cell Research & Therapy
Qiang Li, Woo-Jin Song, Min-Ok Ryu, Aryung Nam, Ju-Hyun An, Jin-Ok Ahn, Dong Ha Bhang, Yun Chan Jung, Hwa-Young Youn
BACKGROUND: Through recent studies, the onset of acute pancreatitis in pancreatic acinar cells (PACs) and the regulatory role of PACs in severe acute pancreatitis (SAP) have been revealed. During the early stages of pancreatitis, the endoplasmic reticulum (ER) in PACs undergoes significant changes, including swelling and vacuolization. In response to an increase in the extracellular stress in ER, PACs lose their functions, leading to cell apoptosis and inflammation response. The beneficial effects of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) on SAP have been well documented in previous studies...
September 26, 2018: Stem Cell Research & Therapy
Arantza Infante, Clara I Rodríguez
Aging is a high risk factor for the development of osteoporosis, a multifactorial age-related progressive disease characterized by reduced bone mass and increased risk of fractures. At the cellular level, the mesenchymal stem cell pool in the bone marrow niche shows a biased differentiation into adipogenesis at the cost of osteogenesis. This differentiation shift leads to decreased bone formation, contributing to the etiology of osteoporosis. This review will focus on the most recent/relevant molecular findings driving this functional impairment of mesenchymal stem cells in the aging process...
September 26, 2018: Stem Cell Research & Therapy
Xin-Yue Zhao, Hua-Tao Xie, Chao-Ye Duan, Jing Li, Ming-Chang Zhang
BACKGROUND: Cultivated oral mucosal epithelial cells (OMECs) are widely used in the treatment of limbal stem cell deficiency (LSCD) for their ocular reconstruction capability. As the most important component of the limbal microenvironment, limbal niche cells (LNCs) play a key role in the direction of stem cell differentiation. In this study, we investigated whether LNCs can induce the transdifferentiation of rat OMECs to corneal epithelial-like cells. METHODS: We isolated OMECs and LNCs from rats by dispase and collagenase, respectively, to establish a three-dimensional or Transwell coculturing system...
September 26, 2018: Stem Cell Research & Therapy
Guping Mao, Ziji Zhang, Shu Hu, Zhiqi Zhang, Zongkun Chang, Zhiyu Huang, Weiming Liao, Yan Kang
BACKGROUND: WNT5A is known to be involved in the pathogenesis of osteoarthritis. This study investigated the molecular mechanism of exosomal miR-92a-3p and WNT5A in chondrogenesis and cartilage degeneration. METHODS: Exosomal miR-92a-3p expression was assessed in vitro in a human mesenchymal stem cell (MSC) model of chondrogenesis and in normal and OA primary human chondrocytes (PHCs). MSCs and PHCs were treated with exosomes derived from MSC-miR-92a-3p (MSC-miR-92a-3p-Exos) or its antisense inhibitor (MSC-anti-miR-92a-3p-Exos), respectively...
September 26, 2018: Stem Cell Research & Therapy
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