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Stem Cell Research & Therapy

Cinzia Rota, Marina Morigi, Domenico Cerullo, Martino Introna, Ornella Colpani, Daniela Corna, Chiara Capelli, Ton J Rabelink, Danielle G Leuning, Daniela Rottoli, Ariela Benigni, Carlamaria Zoja, Giuseppe Remuzzi
BACKGROUND: Mesenchymal stromal cell (MSC)-based therapy is a promising strategy for preventing the progression of chronic kidney disease (CKD), with the potential to induce tissue regeneration. In search of the best cellular source we compared, in the rat model of adriamycin (ADR) nephropathy, the regenerative potential of human stromal cells of non-renal origin, such as bone marrow (bm) MSCs and umbilical cord (uc) MSCs, with that of newly discovered stromal cells of renal origin, the kidney perivascular cells (kPSCs) known to exhibit tissue-specific properties...
August 14, 2018: Stem Cell Research & Therapy
Lijian Shao, Wuping Yang, Rui Xu, Shuqin Zhu, Yanqiu Huang, Huan Li, Xincheng Wu, Mengzhen Yue, Xiaoliang Xiong, Xiaowen Chen, Bohai Kuang, Guangqin Fan, Qingxian Zhu, Huihong Zeng
BACKGROUND: Irradiation-induced kidney damage is inevitable during radiotherapeutic practice, which limits effective radiotherapy doses on tumor treatment. In the present study, the role of mTOR complex 1 (mTORC1) signaling was investigated in irradiation-induced renal injuries. METHODS: Mice were exposed to 8.0-Gy X-ray of total body irradiation and subsequently treated with rapamycin. Changes of renal morphology were assessed by hematoxylin and eosin staining...
August 14, 2018: Stem Cell Research & Therapy
Jianfeng Tang, Xiaoming Wang, Kezhe Tan, Hongtao Zhu, Youming Zhang, Weili Ouyang, Xueqing Liu, Zhaoping Ding
BACKGROUND: Injury may induce a sequential activation of intrinsic reparative activity that supports the maintenance of tissue homeostasis. METHOD: In the present experiments, we investigated whether myocardial infarction (MI) was able to reinstate the expression of Wilms' tumor factor 1 (WT1) as a key hallmark of fetal reprograming in the pericardial adipose-derived stem cells (pADSC). We characterized the immunophenotypical markers, cardiac potential, and reparative activity of WT1-expressing pADSC (WT1pos ) isolated MI Wistar rats with an intact pericardial sac in which cardiac transudate was accumulated, sampled, and analyzed...
August 13, 2018: Stem Cell Research & Therapy
Yena Kim, Narae Park, Yeri Alice Rim, Yoojun Nam, Hyerin Jung, Kijun Lee, Ji Hyeon Ju
BACKGROUND: Skin is an organ that plays an important role as a physical barrier and has many other complex functions. Skin mimetics may be useful for studying the pathophysiology of diseases in vitro and for repairing lesions in vivo. Cord blood mononuclear cells (CBMCs) have emerged as a potential cell source for regenerative medicine. Human induced pluripotent stem cells (iPSCs) derived from CBMCs have great potential for allogenic regenerative medicine. Further study is needed on skin differentiation using CBMC-iPSCs...
August 13, 2018: Stem Cell Research & Therapy
Chongyang Shen, Qingli Quan, Chuan Yang, Yueqiang Wen, Hong Li
BACKGROUND: Adipose-derived mesenchymal stem cells (ADSCs) have been extensively explored as a promising therapeutic agent due to their differentiation, proliferation and migration abilities. The epigenetic mechanisms that regulate the fate of mesenchymal stem cells (MSCs) have been described in detail. However, the epigenetic modulation of ADSCs proliferation and migration is poorly understood. METHODS: The present study examined histone demethylases roles and expression by RT-PCR, as well as through siRNA screening and ChIP-qPCR assay...
August 9, 2018: Stem Cell Research & Therapy
Kyoung-Woon Kim, Hye Joung Kim, Bo-Mi Kim, Yong-Rim Kwon, Hae-Rim Kim, Yoo-Jin Kim
BACKGROUND: The aim of this study was to investigate if epigenetically modified human mesenchymal stromal cells (hMSCs) can regulate the Th17-related immune responses. METHODS: We tested epigenetic drug combinations at various doses and selected the four combinations that resulted in maximal interleukin (IL)-10 and indoleamine 2,3-dioxygenase gene expression in hMSCs. We examined the effects of epigenetically modified hMSCs (epi-hMSCs) on CD4+ T-cell proliferation and inflammatory cytokine secretion under Th0- and Th17-polarizing conditions using mixed lymphocyte reactions and enzyme-linked immunosorbent assays (ELISAs)...
August 9, 2018: Stem Cell Research & Therapy
Cecilie Gjerde, Kamal Mustafa, Sølve Hellem, Markus Rojewski, Harald Gjengedal, Mohammed Ahmed Yassin, Xin Feng, Siren Skaale, Trond Berge, Annika Rosen, Xie-Qi Shi, Aymen B Ahmed, Bjørn Tore Gjertsen, Hubert Schrezenmeier, Pierre Layrolle
BACKGROUND: Autologous grafting, despite some disadvantages, is still considered the gold standard for reconstruction of maxillofacial bone defects. The aim of this study was to evaluate bone regeneration using bone marrow-derived mesenchymal stromal cells (MSCs) in a clinical trial, a less invasive approach than autologous bone grafting. This comprehensive clinical trial included subjects with severe mandibular ridge resorption. METHODS: The study included 11 subjects aged 52-79 years with severe mandibular ridge resorption...
August 9, 2018: Stem Cell Research & Therapy
Dandan Li, Hui Li, Ying Wang, Ahmed Eldomany, Jing Wu, Chao Yuan, Jing Xue, Juan Shi, Yuanyuan Jia, Chunfang Ha, Shuxia Han, Xiaoming Liu, Jiali Yang, Dan Liu
Human endometrial epithelia undergo injury repair and regeneration with the menstrual cycle; however, mechanisms underpinning the roles of endometrial epithelial cells in endometrial lesions and regeneration remain incompletely understood, mainly owing to the difficulty in the isolation and expansion of primary endometrial epithelial cells and the lack of reliable models for in vitro and in vivo studies. In this report, we sought to improve methods for the isolation and expansion of human endometrial epithelial cells with a Rho-associated protein kinase (ROCK) inhibitor-modified medium and subsequently characterize endometrial epithelium generated with primary cells cultured in an air-liquid interface (ALI) state...
August 9, 2018: Stem Cell Research & Therapy
Chang Tu, Yifan Xiao, Yongzhuang Ma, Hua Wu, Mingyu Song
BACKGROUND: The effects of electromagnetic fields (EMF) on bone nonunion have been reported for many years. Many studies and randomized controlled trials have demonstrated that EMF exhibited benefits in curing delayed union and nonunion of long bone fractures. Most of them focused on the immediate effects, while the legacy effects of EMF remain poorly investigated. METHODS: In this study, rat bone marrow mesenchymal stem cells (BMSCs) were treated with EMF, and after a period of time the BMSC proliferation and differentiation were detected...
August 9, 2018: Stem Cell Research & Therapy
Zheying Min, Qian Gao, Xiumei Zhen, Yong Fan, Tao Tan, Rong Li, Yue Zhao, Yang Yu
BACKGROUND: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that affects female fertility. However, with the lack of a corresponding research model, the pathology mechanism of PCOS is poorly understood. Induced pluripotent stem cell (iPSC) technology has been recognized as means to generate patient-specific stem cells for disease modeling. METHODS: The mRNA abundance of iPSCs was analyzed by RNA microarray and real-time polymerase chain reaction (RT-PCR)...
August 9, 2018: Stem Cell Research & Therapy
Ziran Xu, Xia He, Xu Shi, Yuhan Xia, Xiaomei Liu, Haitao Wu, Pengdong Li, Hongyu Zhang, Weisi Yin, Xiubo Du, Lisha Li, Yulin Li
BACKGROUND: Differentiation of human induced pluripotent stem cells (hiPSCs) into hepatocytes has important clinical significance in providing a new stem cell source for cell therapy of terminal liver disease. The differential gene expression analysis of hiPSCs, induced hepatocyte-like cells (HLCs), and primary human hepatocytes (PHHs) provides valuable information for optimization of an induction scheme and exploration of differentiation mechanisms. METHODS: Human hair follicle-derived iPSCs (hHF-iPSCs) were induced in vitro by mimicking the environment of a developing liver for 19 days...
August 9, 2018: Stem Cell Research & Therapy
Barbara Bellei, Emilia Migliano, Marinella Tedesco, Silvia Caputo, Federica Papaccio, Gianluca Lopez, Mauro Picardo
BACKGROUND: Adipose tissue-derived stem cells are considered to be a promising source in the field of cell therapy and regenerative medicine. In addition to direct cell replacement using adipose tissue or purified stem cells, intercellular molecule exchange by the adipose tissue complex, a vast array of bioactive secretory factors, demonstrated beneficial effects by reducing tissue damage and stimulation of endogenous repair. However, for therapeutic purposes, the use of secretome derivatives, such as full conditioned media or purified exosomes generated in vitro, may present considerable disadvantages for cell manufacturing, storage, product safety, and their potential as a ready-to-go therapeutic product...
August 9, 2018: Stem Cell Research & Therapy
Boxian Huang, Jiafeng Lu, Chenyue Ding, Qinyan Zou, Wei Wang, Hong Li
BACKGROUND: Although many reports show that various kinds of stem cells have the ability to recover the function of premature ovarian insufficiency (POI), few studies are associated with the mechanism of stem cell treatment of POI. We designed this experimental study to investigate whether human adipose stem cell-derived exosomes (hADSC-Exos) retain the ability to restore ovarian function and how hADSC-Exos work in this process. METHODS: A POI mouse model was established and human ovarian granule cells (hGCs) collected from individuals with POI were prepared to assess the therapeutic effects and illuminate the mechanism of hADSCs in curing POI...
August 9, 2018: Stem Cell Research & Therapy
Chen Li, Zhuoquan Li, Yan Zhang, Abdel Hamid Fathy, Min Zhou
BACKGROUND: Several studies have confirmed that gold nanoparticles (AuNPs) of specific concentration and size exert a boosting effect on cell proliferation; however, the mechanism through which this effect occurs remains unknown. This study explores the canonical Wnt signaling pathway in AuNP promotion of human periodontal ligament stem cell (hPDLSC) proliferation. METHODS: MTS was employed to evaluate hPDLSC proliferation. The interference of LRP5 and β-catenin was steered by shRNA plasmids and siRNA, respectively, at which point the expression of MYC, CCND1, AXIN2, and POU5F1 had been estimated via real-time PCR...
August 9, 2018: Stem Cell Research & Therapy
Samuel E Taylor, Ruth D Taylor, Jack Price, Laura C Andreae
BACKGROUND: Deletions and mutations in the SHANK3 gene are strongly associated with autism spectrum disorder and underlie the autism-associated disorder Phelan-McDermid syndrome. SHANK3 is a scaffolding protein found at the post-synaptic membrane of excitatory neurons. METHODS: Single-molecule fluorescence in-situ hybridization (smFISH) allows the visualization of single mRNA transcripts in vitro. Here we perform and quantify smFISH in human inducible pluripotent stem cell (hiPSC)-derived cortical neurons, targeting the SHANK3 transcript...
July 31, 2018: Stem Cell Research & Therapy
Alexia Mattei, Jérémy Magalon, Baptiste Bertrand, Fanny Grimaud, Joana Revis, Mélanie Velier, Julie Veran, Patrick Dessi, Florence Sabatier, Antoine Giovanni
No abstract text is available yet for this article.
July 27, 2018: Stem Cell Research & Therapy
Fuyu Duan, Rujin Huang, Fengzhi Zhang, Yonglin Zhu, Lin Wang, Xia Chen, Lufeng Bai, Wei Guo, Sophia Chia-Ning Chang, Xiaoyu Hu, Jie Na
BACKGROUND: Hematopoietic lineage cells derived from human pluripotent stem cells (hPSCs) hold great promise for the treatment of hematological diseases and providing sufficient cells for immune therapy. However, a simple, cost-effective method to generate large quantities of hematopoietic stem/progenitor cells (HSPCs) is not yet available. METHODS: We established a monolayer, chemically defined culture system to induce hematopoietic differentiation from hPSCs in 8 days...
July 27, 2018: Stem Cell Research & Therapy
Sean K Sweeney, Gohar S Manzar, Nicholas Zavazava, Jose G Assouline
BACKGROUND: As the prevalence of therapeutic approaches involving transplanted cells increases, so does the need to noninvasively track the cells to determine their homing patterns. Of particular interest is the fate of transplanted embryonic stem cell-derived hematopoietic progenitor cells (HPCs) used to restore the bone marrow pool following sublethal myeloablative irradiation. The early homing patterns of cell engraftment are not well understood at this time. Until now, longitudinal studies were hindered by the necessity to sacrifice several mice at various time points of study, with samples of the population of lymphoid compartments subsequently analyzed by flow cytometry or fluorescence microscopy...
July 27, 2018: Stem Cell Research & Therapy
Haissam Abou-Saleh, Fouad A Zouein, Ahmed El-Yazbi, Despina Sanoudou, Christophe Raynaud, Christopher Rao, Gianfranco Pintus, Hassan Dehaini, Ali H Eid
Cardiovascular disease (CVD) continues to be the leading cause of global morbidity and mortality. Heart failure remains a major contributor to this mortality. Despite major therapeutic advances over the past decades, a better understanding of molecular and cellular mechanisms of CVD as well as improved therapeutic strategies for the management or treatment of heart failure are increasingly needed. Loss of myocardium is a major driver of heart failure. An attractive approach that appears to provide promising results in reducing cardiac degeneration is stem cell therapy (SCT)...
July 27, 2018: Stem Cell Research & Therapy
Hanna Hanna, Lluis M Mir, Franck M Andre
BACKGROUND: Differentiation of mesenchymal stem cells to osteoblasts is widely performed in research laboratories. Classical tests to prove this differentiation employ procedures such as cell fixation, cell lysis or cell scraping. Very few studies report gentle dissociation of mesenchymal stem cells undergoing an osteodifferentiation process. Here we used this technique to reveal the presence of several cell layers during osteogenesis and to study their different properties. METHODS: Through the sequential enzymatic detachment of the cells, we confirm the presence of several layers of differentiated cells and we compare them in terms of enzymatic sensitivity for dissociation, expression of cluster of differentiation, cytosolic calcium oscillations and osteogenic potential...
July 27, 2018: Stem Cell Research & Therapy
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