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Journal of Parasitology Research

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https://www.readbyqxmd.com/read/29318036/cysticercus-fasciolaris-in-brown-rats-rattus-norvegicus-in-grenada-west-indies
#1
Ravindra Sharma, Keshaw Tiwari, Kristen Birmingham, Elan Armstrong, Andrea Montanez, Reneka Guy, Yvette Sepulveda, Veronica Mapp-Alexander, Claude DeAllie
Cat is the definitive host of Taenia taeniaeformis (T. taeniaeformis). Cysticercus fasciolaris (C. fasciolaris), the larval stage of T. taeniaeformis, develops in small rodents which act as intermediate host. The aim of this study was to estimate the prevalence of C. fasciolaris in brown rats (Rattus norvegicus) in the densely human populated parishes, St. George's and St. David's of Grenada, West Indies. One hundred and seventy rats were trapped near the residential areas from May to July, 2017 and examined for C...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29250439/the-effect-of-deworming-using-triple-dose-albendazole-on-nutritional-status-of-children-in-perobatang-village-southwest-sumba-indonesia
#2
Saleha Sungkar, Asri S Ridwan, Gladys Kusumowidagdo
High prevalence of STH leads to malnutrition, anemia, cognitive impairment, and growth disorders. Triple-dose albendazole 400 mg is a broad-spectrum anthelminthic; however, its effectiveness varies in every region. This study aims to determine the benefits of deworming using triple-dose albendazole on children's nutritional status in Perobatang Village, Southwest Sumba, Indonesia. This pre-post study was conducted in July 2016 and January 2017. Children aged 1-15 years were asked to collect stool for diagnosis of STH infection (Kato-Katz method), were measured for anthropometry status to obtain the nutritional status, and took albendazole 400 mg for three consecutive days...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29238615/profile-of-geohelminth-eggs-cysts-and-oocysts-of-protozoans-contaminating-the-soils-of-ten-primary-schools-in-dschang-west-cameroon
#3
Vanessa Rosine Nkouayep, Blandine Ngatou Tchakounté, Josué Wabo Poné
Helminthiasis and protozoans infections have been recognized as an important public health problem. The aim of the present study was to screen soil samples collected from 10 primary schools in the city of Dschang for the presence of soil-transmitted helminth eggs, cysts, and oocysts of protozoans. A total of 400 soil samples were collected around latrines, at playgrounds, and behind classrooms in each school. These samples were examined using the sucrose flotation method. From the result obtained, an overall contamination rate of 7...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29225962/tlr-specific-immune-responses-against-helminth-infections
#4
REVIEW
Sivaprakasam Rajasekaran, Rajamanickam Anuradha, Ramalingam Bethunaickan
Despite marked improvement in the quality of lives across the globe, more than 2 million individuals in socioeconomically disadvantaged environments remain infected by helminth (worm) parasites. Owing to the longevity of the worms and paucity of immunologic controls, these parasites survive for long periods within the bloodstream, lymphatics, and gastrointestinal tract resulting in pathologic conditions such as anemia, cirrhosis, and lymphatic filariasis. Despite infection, an asymptomatic state may be maintained by the host immunoregulatory environment, which involves multiple levels of regulatory cells and cytokines; a breakdown of this regulation is observed in pathological disease...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29181192/selecting-pcr-for-the-diagnosis-of-intestinal-parasitosis-choice-of-targets-evaluation-of-in-house-assays-and-comparison-with-commercial-kits
#5
G N Hartmeyer, S V Hoegh, M N Skov, R B Dessau, M Kemp
Microscopy of stool samples is a labour-intensive and inaccurate technique for detection of intestinal parasites causing diarrhoea and replacement by PCR is attractive. Almost all cases of diarrhoea induced by parasites over a nine-year period in our laboratory were due to Giardia lamblia, Cryptosporidium species, or Entamoeba histolytica detected by microscopy. We evaluated and selected in-house singleplex real-time PCR (RT-PCR) assays for these pathogens in 99 stool samples from patients suspected of having intestinal parasitosis tested by microscopy...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29138693/investigations-on-the-occurrence-and-associated-risk-factors-of-avian-coccidiosis-in-osun-state-southwestern-nigeria
#6
Shola David Ola-Fadunsin
Avian coccidiosis is one of the most important diseases of poultry and it is responsible for a large number of poultry mortalities worldwide. This study was carried out to investigate the occurrence and associated risk factors of avian coccidiosis in Osun State, Nigeria. Fecal samples were collected and examined from 5,544 avian species that were brought for treatment at the state veterinary hospitals over a 10-year period. Parameters such as age, sex, season, and species of birds were determined. Also, the months of the year were taken into consideration...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29082037/new-scenarios-of-chagas-disease-transmission-in-northern-colombia
#7
Catalina Tovar Acero, Jorge Negrete Peñata, Camila González, Cielo León, Mario Ortiz, Julio Chacón Pacheco, Elkin Monterrosa, Abraham Luna, Dina Ricardo Caldera, Lyda Espitia-Pérez
Chagas disease (CD) is a systemic parasitic infection caused by the flagellated form of Trypanosoma cruzi. Córdoba department, located in the Colombian Caribbean Coast, was not considered as a region at risk of T. cruzi transmission. In this article, we describe the first acute CD case in Salitral village in Sahagún, Córdoba, confirmed by microscopy and serological tests. Our results draw attention to a new scenario of transmission of acute CD in nonendemic areas of Colombia and highlight the need to include CD in the differential diagnosis of febrile syndromes in this region...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/29057116/prevalence-of-intestinal-helminths-infestation-in-children-attending-princess-marie-louise-children-s-hospital-in-accra-ghana
#8
Robert Mirisho, Margaret L Neizer, Bismark Sarfo
The deworming exercise program does not cover all children who are not in school. This study determined the prevalence and species type of helminth infestation and associated factors among children attending Princess Marie Louise Children's Hospital in Accra, Ghana. Children (225) below the age of 10 who have not taken antihelminthic drugs prior to the study period were recruited between May and June 2015. Children or guardians were interviewed using structured questionnaires and fresh stools were collected and processed for helminths species identification using microscopy...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28912965/medically-important-parasites-carried-by-cockroaches-in-melong-subdivision-littoral-cameroon
#9
R J Atiokeng Tatang, H G Tsila, J Wabo Poné
Cockroaches have been recognized as mechanical vectors of pathogens that can infest humans or animals. A total of 844 adult cockroaches (436 males and 408 females) were caught. In the laboratory, cockroaches were first washed in saturated salt solution to remove ectoparasites and then rinsed with 70% alcohol, dried, and dissected for endoparasites. An overall transport rate of 47.39% was recorded. Six genera of parasites were identified. These were Ascaris (33.76%), Trichuris (11.97%), Capillaria (6.16%), Toxocara (4...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28884022/identification-characterization-and-structure-of-tm16-from-trichuris-muris
#10
Zhuyun Liu, Alan Kelleher, Shanii Tabb, Junfei Wei, Jeroen Pollet, Peter J Hotez, Maria Elena Bottazzi, Bin Zhan, Oluwatoyin A Asojo
Trichuriasis is a disease of poverty for which excretory and secretory (ES) products that induce the protective immunity are being investigated as candidate vaccines antigens. In this study, ES products of T. muris and immune sera were produced. The immune sera recognized more than 20 proteins on a 2D-gel of ES products of T. muris adult worms. Tm16 was one of the proteins identified by mass spectrometry. Tm16 shares 57% sequence identity with Ov16, an immunodominant diagnostic antigen from Onchocerca volvulus...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28656101/a-brief-view-of-the-surface-membrane-proteins-from-trypanosoma-cruzi
#11
REVIEW
Ángel de la Cruz Pech-Canul, Victor Monteón, Rosa-Lidia Solís-Oviedo
Trypanosoma cruzi is the causal agent of Chagas' disease which affects millions of people around the world mostly in Central and South America. T. cruzi expresses a wide variety of proteins on its surface membrane which has an important role in the biology of these parasites. Surface molecules of the parasites are the result of the environment to which the parasites are exposed during their life cycle. Hence, T. cruzi displays several modifications when they move from one host to another. Due to the complexity of this parasite's cell surface, this review presents some membrane proteins organized as large families, as they are the most abundant and/or relevant throughout the T...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28611925/prevalence-and-correlates-of-intestinal-parasites-among-patients-admitted-to-mirembe-national-mental-health-hospital-dodoma-tanzania
#12
Azan A Nyundo, David Z Munisi, Ainory P Gesase
BACKGROUND: Neglected tropical diseases continue to be one of the leading causes of morbidity and mortality in the developing world. Psychiatric patients are among groups at risk for parasitic infection although control and monitoring programs largely overlook this population. This study aimed at determining prevalence and factors associated with intestinal parasitic infection among patients admitted to a psychiatric facility. METHOD: The study followed cross-sectional design; all the residing patients that met the inclusion criteria were included in the survey...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28348881/in-vitro-assessment-of-anthelmintic-activities-of-rauwolfia-vomitoria-apocynaceae-stem-bark-and-roots-against-parasitic-stages-of-schistosoma-mansoni-and-cytotoxic-study
#13
Emmanuel Mouafo Tekwu, Kwabena Mante Bosompem, William Kofi Anyan, Regina Appiah-Opong, Kofi Baffour-Awuah Owusu, Mabel Deladem Tettey, Felicia Amanfo Kissi, Alfred Ampomah Appiah, Veronique Penlap Beng, Alexander Kwadwo Nyarko
Schistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots of Rauwolfia vomitoria against two life stages of Schistosoma mansoni. Both plant parts were found to be active against cercariae and adult worms. Within 2 h of exposure all cercariae were killed at a concentration range of 62...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28286673/construction-of-two-mcherry-plasmids-pxg-mcherry-for-transgenic-leishmania-valuable-tools-for-future-molecular-analysis
#14
Andrés Vacas, Conor Sugden, Óscar Velasco-Rodriguez, Miriam Algarabel-Olona, José Peña-Guerrero, Esther Larrea, Celia Fernández-Rubio, Paul A Nguewa
Leishmania is the causative agent of leishmaniasis, a neglected tropical disease that affects more than 12 million people around the world. Current treatments are toxic and poorly effective due to the acquisition of resistance within Leishmania populations. Thus, the pursuit for new antileishmanial drugs is a priority. The available methods for drug screening based on colorimetric assays using vital dyes are time-consuming. Currently, the use of fluorescent reporter proteins is replacing the use of viability indicator dyes...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28210503/strongyloidiasis-current-status-with-emphasis-in-diagnosis-and-drug-research
#15
REVIEW
Tiago Mendes, Karen Minori, Marlene Ueta, Danilo Ciccone Miguel, Silmara Marques Allegretti
Strongyloidiasis is a parasitic neglected disease caused by the nematode Strongyloides stercoralis affecting 30 to 100 million people worldwide. Complications, strongly associated with alcoholism, organ transplants, and HTLV-1 virus, often arise due to late diagnosis, frequently leading to patient death. Lack of preemptive diagnosis is not the only difficulty when dealing with this parasite, since there are no gold standard diagnostic techniques, and the ones used have problems associated with sensitivity, resulting in false negatives...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28168042/coinfection-with-schistosoma-haematobium-and-plasmodium-falciparum-and-anaemia-severity-among-pregnant-women-in-munyenge-mount-cameroon-area-a-cross-sectional-study
#16
Judith K Anchang-Kimbi, Dillys Mansoh Elad, Gemain Taiwe Sotoing, Eric Akum Achidi
Background. Malaria and urogenital schistosomiasis are coendemic in Mount Cameroon Area. This study investigated the prevalence of S. haematobium, P. falciparum, and coinfections and their effect on anaemia in pregnancy. Methods. Pregnant women reporting for antenatal care (ANC) clinic visit in Munyenge were enrolled. S. haematobium and P. falciparum infections were determined by urine filtration and microscopy, respectively. Haemoglobin (Hb) levels were measured using haemoglobinometer. Of 250 women, 46.8%, 39...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27980861/molecular-evidence-of-high-proportion-of-plasmodium-vivax-malaria-infection-in-white-nile-area-in-sudan
#17
Makarim M Adam Suliman, Bushra M Hamad, Musab M Ali Albasheer, Maytha Elhadi, Mutaz Amin Mustafa, Maha Elobied, Muzamil Mahdi Abdel Hamid
Plasmodium falciparum is a predominant malaria species that infects humans in the African continent. A recent WHO report estimated 95% and 5% of P. falciparum and P. vivax malaria cases, respectively, in Sudan. However many laboratory reports from different areas in Sudan indicated otherwise. In order to verify, we selected four hundred suspected malaria cases from Aljabalain area located in the White Nile state, central Sudan, and diagnosed them with quality insured microscopy and species-specific nested PCR...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27882242/development-of-a-loop-mediated-isothermal-amplification-for-diagnosis-of-ascaris-lumbricoides-in-fecal-samples
#18
Esther A Shiraho, Agola L Eric, Ibrahim N Mwangi, Geoffrey M Maina, Joseph M Kinuthia, Martin W Mutuku, Robert M Mugambi, Jackson M Mwandi, Gerald M Mkoji
Ascaris lumbricoides is a nematode parasite that causes the common tropical infection ascariasis in humans. It is also considered among the neglected tropical diseases. Diagnosis relies mainly on microscopy-based methods which are laborious, are limited by low sensitivity, and require high expertise. We have developed a loop mediated isothermal amplification (LAMP) for diagnosis of ascariasis in fecal samples, based on the first internal transcribed (ITS-1) spacer region of the ribosomal DNA. We used Primer Explorer V4 software to design primers...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27882241/immune-profile-of-honduran-schoolchildren-with-intestinal-parasites-the-skewed-response-against-geohelminths
#19
José Antonio Gabrie, María Mercedes Rueda, Carol Anahelka Rodríguez, Maritza Canales, Ana Lourdes Sanchez
Soil-transmitted helminth infections typically induce a type-2 immune response (Th2), but no immunoepidemiological studies have been undertaken in Honduras, an endemic country where the main control strategy is children's annual deworming. We aimed to characterize the immune profile of Honduran schoolchildren harbouring these parasitoses. Demographic and epidemiological data were obtained through a survey; nutritional status was assessed through anthropometry; intestinal parasites were diagnosed by formol-ether and Kato-Katz; and blood samples were collected to determine immunological markers including Th1/Th2 cytokines, IgE, and eosinophil levels...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27872753/multifunctional-thioredoxin-like-protein-from-the-gastrointestinal-parasitic-nematodes-strongyloides-ratti-and-trichuris-suis-affects-mucosal-homeostasis
#20
Dana Ditgen, Emmanuela M Anandarajah, Jan Hansmann, Dominic Winter, Guido Schramm, Klaus D Erttmann, Eva Liebau, Norbert W Brattig
The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S) products of Strongyloides ratti and Trichuris suis sequences for thioredoxin (Trx) and Trx-like protein (Trx-lp) were identified. To characterize the antioxidant Trx-lp and its interaction with the parasite's mucosal habitat, S. ratti and T. suis Trx-lps were cloned and recombinantly expressed. The primary antioxidative activity was assured by reduction of insulin and IgM...
2016: Journal of Parasitology Research
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