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Journal of Parasitology Research

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https://www.readbyqxmd.com/read/27882242/development-of-a-loop-mediated-isothermal-amplification-for-diagnosis-of-ascaris-lumbricoides-in-fecal-samples
#1
Esther A Shiraho, Agola L Eric, Ibrahim N Mwangi, Geoffrey M Maina, Joseph M Kinuthia, Martin W Mutuku, Robert M Mugambi, Jackson M Mwandi, Gerald M Mkoji
Ascaris lumbricoides is a nematode parasite that causes the common tropical infection ascariasis in humans. It is also considered among the neglected tropical diseases. Diagnosis relies mainly on microscopy-based methods which are laborious, are limited by low sensitivity, and require high expertise. We have developed a loop mediated isothermal amplification (LAMP) for diagnosis of ascariasis in fecal samples, based on the first internal transcribed (ITS-1) spacer region of the ribosomal DNA. We used Primer Explorer V4 software to design primers...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27882241/immune-profile-of-honduran-schoolchildren-with-intestinal-parasites-the-skewed-response-against-geohelminths
#2
José Antonio Gabrie, María Mercedes Rueda, Carol Anahelka Rodríguez, Maritza Canales, Ana Lourdes Sanchez
Soil-transmitted helminth infections typically induce a type-2 immune response (Th2), but no immunoepidemiological studies have been undertaken in Honduras, an endemic country where the main control strategy is children's annual deworming. We aimed to characterize the immune profile of Honduran schoolchildren harbouring these parasitoses. Demographic and epidemiological data were obtained through a survey; nutritional status was assessed through anthropometry; intestinal parasites were diagnosed by formol-ether and Kato-Katz; and blood samples were collected to determine immunological markers including Th1/Th2 cytokines, IgE, and eosinophil levels...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27872753/multifunctional-thioredoxin-like-protein-from-the-gastrointestinal-parasitic-nematodes-strongyloides-ratti-and-trichuris-suis-affects-mucosal-homeostasis
#3
Dana Ditgen, Emmanuela M Anandarajah, Jan Hansmann, Dominic Winter, Guido Schramm, Klaus D Erttmann, Eva Liebau, Norbert W Brattig
The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S) products of Strongyloides ratti and Trichuris suis sequences for thioredoxin (Trx) and Trx-like protein (Trx-lp) were identified. To characterize the antioxidant Trx-lp and its interaction with the parasite's mucosal habitat, S. ratti and T. suis Trx-lps were cloned and recombinantly expressed. The primary antioxidative activity was assured by reduction of insulin and IgM...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27830083/the-seroprevalence-of-human-cystic-echinococcosis-in-iran-a-systematic-review-and-meta-analysis-study
#4
REVIEW
Reza Shafiei, Saeed Hosseini Teshnizi, Kurosh Kalantar, Maryam Gholami, Golnush Mirzaee, Fatemeh Mirzaee
Human cystic echinococcosis (HCE), a zoonotic infection of the larval stage of Echinococcus granulosus, has high effect on public health in human population all around the world. Iran is one of the most important endemic areas in the Middle East. This systematic review and meta-analysis was performed to evaluate the seroprevalence of HCE in Iranian population. An electronic search for articles from 1985 until April 2015 was performed using data bases PubMed, Scopus, Google Scholar, Magiran, IranMedex, Iran Doc, and Scientific Information Database (SID) both in English and in Persian...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27822385/intestinal-schistosomiasis-among-primary-schoolchildren-in-two-on-shore-communities-in-rorya-district-northwestern-tanzania-prevalence-intensity-of-infection-and-associated-risk-factors
#5
David Z Munisi, Joram Buza, Emmanuel A Mpolya, Safari M Kinung'hi
In Tanzania, Schistosoma mansoni is of great public health importance. Understanding the prevalence and infection intensity is important for targeted, evidence-based control strategies. This study aimed at studying the prevalence, intensity, and risk factors of S. mansoni among schoolchildren in the study area. A cross-sectional study was conducted in Busanga and Kibuyi villages. Sampled 513 schoolchildren provided stool specimens which were examined using kato-katz method. Pretested questionnaire was used to collect sociodemographic data and associated risk factors...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27747097/comparative-performance-evaluation-of-routine-malaria-diagnosis-at-ho-municipal-hospital
#6
James Osei-Yeboah, Gameli Kwame Norgbe, Sylvester Yao Lokpo, Mohammed Khadijah Kinansua, Loverage Nettey, Emmanuel Alote Allotey
Differences in quality performance score had been reported for the routinely used diagnostic methods for malaria at different settings. There is therefore a need to evaluate the test performance of the routine diagnostic methods for malaria detection in Ho, a setting with no recorded quality evaluation on malaria diagnosis. The hospital-based cross-sectional study was conducted comprising 299 outpatients. Patients were first seen and presumptively diagnosed with malaria by a clinician and were referred to the laboratory for confirmation (microscopy and Rapid Diagnostic Test)...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27738522/distribution-of-helminth-parasites-in-intestines-and-their-seasonal-rate-of-infestation-in-three-freshwater-fishes-of-kashmir
#7
Asifa Wali, Masood-Ul Hassan Balkhi, Rafia Maqbool, Mohammed Maqbool Darzi, Feroz Ahmad Shah, Farooz Ahmad Bhat, Bilal Ahmad Bhat
The present study was undertaken to determine the incidence of helminth parasites in fishes with special reference to water quality parameters in Dal Lake and River Jhelum and correlate the observations. Water, fish, and parasite samples were collected during different seasons from various sites and processed. Three fish species, namely, Schizothorax niger Heckel 1838, Schizothorax esocinus Heckel 1838, and Schizothorax curvifrons Heckel 1838, were recovered from these water bodies. The physicochemical parameters temperature, dissolved oxygen, pH, and free carbon dioxide showed variation vis-à-vis the season and location of the stations in water bodies...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27597893/prevalence-and-scanning-electron-microscopic-identification-of-anoplocephalid-cestodes-among-small-ruminants-in-senegal
#8
Mallé Ndom, Gora Diop, Yann Quilichini, Tetsuya Yanagida, Cheikh Tidiane Ba, Bernard Marchand
This study was undertaken to determine the prevalence of anoplocephalid cestodes in sheep and goats in Senegal. Intestines of 462 sheep and 48 goats were examined; 47.4% of sheep and 6.2% of goats were infected. The species identified and their prevalence were, among sheep, Avitellina centripunctata 38.7%, Moniezia expansa 15.4%, Stilesia globipunctata 16.7%, and Thysaniezia ovilla 0.4%. Among goats, they were M. expansa 6.2% and T. ovilla 2.1%. The prevalence of all species was not statistically different between dry and rainy seasons...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27597892/could-kdna-pcr-in-peripheral-blood-replace-the-examination-of-bone-marrow-for-the-diagnosis-of-visceral-leishmaniasis
#9
Natalia Souza de Godoy, Marcos Luiz Alves Andrino, Regina Maia de Souza, Erika Gakiya, Valdir Sabbaga Amato, José Ângelo Lauletta Lindoso, Lucia Maria Almeida Braz
The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL). PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM. Based on the GS, 38 samples from 32 patients were grouped: Group 1, 20 samples of VL cases, and Group 2, 18 samples of non-VL cases...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27559480/checklists-of-parasites-of-farm-fishes-of-babylon-province-iraq
#10
REVIEW
Furhan T Mhaisen, Abdul-Razzak L Al-Rubaie
Literature reviews of all references concerning the parasitic fauna of fishes in fish farms of Babylon province, middle of Iraq, showed that a total of 92 valid parasite species are so far known from the common carp (Cyprinus carpio), the grass carp (Ctenopharyngodon idella), and the silver carp (Hypophthalmichthys molitrix) as well as from three freshwater fish species (Carassius auratus, Liza abu, and Heteropneustes fossilis) which were found in some fish farms of the same province. The parasitic fauna included one mastigophoran, three apicomplexans, 13 ciliophorans, five myxozoans, five trematodes, 45 monogeneans, five cestodes, three nematodes, two acanthocephalans, nine arthropods, and one mollusc...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27525108/current-status-of-soil-transmitted-helminths-among-school-children-in-kakamega-county-western-kenya
#11
Teresia Ngonjo, Collins Okoyo, Julius Andove, Elses Simiyu, Agola Eric Lelo, Ephantus Kabiru, Jimmy Kihara, Charles Mwandawiro
Background. School age children are at high risk of soil-transmitted helminth (STH) worldwide. In Kenya, STH infections in children remain high despite the periodic administration of anthelmintic drugs. Our study assessed the prevalence and intensity of STH in primary school-aged children in Kakamega County, western Kenya. Methodology. We carried out a cross-sectional study on a population of 731 children attending 7 primary schools in March 2014. Children aged 4-16 years were examined for STH by the quantitative Kato-Katz technique...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27313865/vectors-and-spatial-patterns-of-angiostrongylus-cantonensis-in-selected-rice-farming-villages-of-mu%C3%A3-oz-nueva-ecija-philippines
#12
Ma Angelica A Tujan, Ian Kendrich C Fontanilla, Vachel Gay V Paller
In the Philippines, rats and snails abound in agricultural areas as pests and source of food for some of the local people which poses risks of parasite transmission to humans such as Angiostrongylus cantonensis. This study was conducted to determine the extent of A. cantonensis infection among rats and snails collected from rice-farming villages of Muñoz, Nueva Ecija. A total of 209 rats, 781 freshwater snails, and 120 terrestrial snails were collected for the study. Heart and lungs of rats and snail tissues were examined and subjected to artificial digestion for parasite collection...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27239333/horizontal-gene-transfers-from-bacteria-to-entamoeba-complex-a-strategy-for-dating-events-along-species-divergence
#13
Miguel Romero, R Cerritos, Cecilia Ximenez
Horizontal gene transfer has proved to be relevant in eukaryotic evolution, as it has been found more often than expected and related to adaptation to certain niches. A relatively large list of laterally transferred genes has been proposed and evaluated for the parasite Entamoeba histolytica. The goals of this work were to elucidate the importance of lateral gene transfer along the evolutionary history of some members of the genus Entamoeba, through identifying donor groups and estimating the divergence time of some of these events...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27190634/neobenedenia-melleni-parasite-of-red-snapper-lutjanus-erythropterus-with-regression-statistical-analysis-between-fish-length-temperature-and-parasitic-intensity-in-infected-fish-cultured-at-jerejak-island-penang-malaysia
#14
Rajiv Ravi, Zary Shariman Yahaya
The fish parasites collected from Lutjanus erythropterus fish species showed a correlation with parasitic intensity, fish size, and temperature, and statistical model summary was produced using SPSS version 20, statistical software. Statistical model summary concluded that among the variables which significantly predict the prevalence of Neobenedenia melleni parasites are fish length and water temperature, both significant at 1% and 5%. Furthermore, the increase in one unit of fish length, holding other variables constant, increases the prevalence of parasite by approximately 1 (0...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27127643/prevalence-of-intestinal-parasitic-infection-among-food-handlers-in-northwest-iran
#15
Davoud Balarak, Mohammad Jafari Modrek, Edris Bazrafshan, Hossein Ansari, Ferdos Kord Mostafapour
Parasitic diseases are among the most important infectious diseases and pose health problems in many countries, most especially in developing countries. Workers at food centers could transmit parasitic infections in the absence of sanitation. This is a descriptive study conducted to determine the prevalence of intestinal parasitic infections in food clerks in the city of Tabriz in 2014. Data was recorded in the offices of the health center for all food handlers who were referred to the laboratory for demographic and stool tests to receive the health card...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27110390/genetic-polymorphism-of-msp1-and-msp2-in-plasmodium-falciparum-isolates-from-c%C3%A3-te-d-ivoire-versus-gabon
#16
William Yavo, Abibatou Konaté, Denise Patricia Mawili-Mboumba, Fulgence Kondo Kassi, Marie L Tshibola Mbuyi, Etienne Kpongbo Angora, Eby I Hervé Menan, Marielle K Bouyou-Akotet
Introduction. The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods. Isolates collected from 100 and 73 falciparum malaria infections in sites of Côte d'Ivoire (West Africa) and Gabon (Central Africa), respectively, were analyzed by a nested PCR amplification of msp1 and msp2 genes. Results. The K1 allelic family was widespread in Côte d'Ivoire (64...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/27042341/quality-of-sulfadoxine-pyrimethamine-given-as-antimalarial-prophylaxis-in-pregnant-women-in-selected-health-facilities-in-central-region-of-ghana
#17
Danny F Yeboah, Richmond Afoakwah, Ekene K Nwaefuna, Orish Verner, Johnson N Boampong
The use of sulfadoxine-pyrimethamine (SP) as an intermittent preventive treatment (IPT) against malaria during pregnancy has become a policy in most sub-Sahara African countries and crucially depends on the efficacy of SP. This study sets out to evaluate the effectiveness of the SP given to the pregnant women in some selected health facilities in the Central Region of Ghana to prevent maternal malaria in pregnant women. A total of 543 pregnant women recruited from 7 selected health centres in Central Region of Ghana participated in the study...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/26989496/therapeutic-and-safety-evaluation-of-combined-aqueous-extracts-of-azadirachta-indica-and-khaya-senegalensis-in-chickens-experimentally-infected-with-eimeria-oocysts
#18
J G Gotep, J T Tanko, G E Forcados, I A Muraina, N Ozele, B B Dogonyaro, O O Oladipo, M S Makoshi, O B Akanbi, H Kinjir, A L Samuel, T E Onyiche, G O Ochigbo, O B Aladelokun, H A Ozoani, V Z Viyoff, C C Dapuliga, A A Atiku, P A Okewole, D Shamaki, M S Ahmed, C I Nduaka
Coccidiosis is a disease of economic importance in poultry causing morbidity and mortality. Reports show that Azadirachta indica and Khaya senegalensis have been used individually in the treatment of avian coccidiosis. We thus investigated the efficacy and safety of the combined aqueous extracts of these plants for the treatment of experimentally induced coccidiosis in broiler chickens using oocyst count, oxidative stress biomarkers, serum biochemistry, histology, and haematological parameters. The phytochemical screening revealed the presence of tannins, saponins, cardiac glycosides, and steroids in both extracts...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/26981275/feeding-patterns-and-xenomonitoring-of-trypanosomes-among-tsetse-flies-around-the-gashaka-gumti-national-park-in-nigeria
#19
Solomon Ngutor Karshima, Idris A Lawal, Oluseyi Oluyinka Okubanjo
In order to understand the epidemiology of trypanosomoses in Gashaka-Gumti National Park, Nigeria, we determined the density, infection rates, and feeding patterns of tsetse flies using biconical traps, ITS, and mitochondrial cytochrome b PCRs. A total of 631 tsetse flies were captured, of which 531 (84.2%) and 100 (15.8%) were analyzed for trypanosomes and blood meals, respectively. Tsetse distribution varied significantly (p < 0.05) across study sites with average trap and daily catches of 4.39 and 26...
2016: Journal of Parasitology Research
https://www.readbyqxmd.com/read/26941997/soil-transmitted-helminth-reinfection-and-associated-risk-factors-among-school-age-children-in-chencha-district-southern-ethiopia-a-cross-sectional-study
#20
Zerihun Zerdo, Tsegaye Yohanes, Befikadu Tariku
Mass drug administration (MDA) to the most risky population including school-age children (SAC) is the central strategy to control soil-transmitted helminth (STH) infection. The present study was aimed at estimating the prevalence of STHs reinfection three months posttreatment and associated risk factors among SAC in Chencha district. A cross-sectional study design was employed from April 20 to May 5, 2015, to enroll 408 SAC. Structured questionnaire and Kato-Katz thick smear technique were used to interview parents or guardians and quantify the number of eggs per gram of stool...
2016: Journal of Parasitology Research
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