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Acta Naturae

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https://www.readbyqxmd.com/read/30116621/role-of-the-scaffold-protein-mim-in-the-actin-dependent-regulation-of-epithelial-sodium-channels-enac
#1
L S Shuyskiy, V V Levchenko, Y A Negulyaev, A V Staruschenko, D V Ilatovskaya
Epithelial Sodium Channels (ENaCs) are expressed in different organs and tissues, particularly in the cortical collecting duct (CCD) in the kidney, where they fine tune sodium reabsorption. Dynamic rearrangements of the cytoskeleton are one of the common mechanisms of ENaC activity regulation. In our previous studies, we showed that the actin-binding proteins cortactin and Arp2/3 complex are involved in the cytoskeleton-dependent regulation of ENaC and that their cooperative work decreases a channel's probability of remaining open; however, the specific mechanism of interaction between actin-binding proteins and ENaC is unclear...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116620/atp-reduces-the-entry-of-calcium-ions-into-the-nerve-ending-by-blocking-l-type-calcium-channels
#2
E F Khaziev, D V Samigullin, A N Tsentsevitsky, E A Bukharaeva, E E Nikolsky
At neuromuscular junctions, ATP inhibits both the evoked and spontaneous acetylcholine release and inward calcium current operating via presynaptic P2Y receptors. It was shown in the experiments with the frog neuromuscular synapse using specific calcium-sensitive dye Oregon Green Bapta 1 that exogenous ATP reduces the amplitude of calcium transient, which reflects the changes in the entry of calcium ions in response to the nerve pulse. The depressing effect of ATP on the transient was prevented by suramin, the blocker of P2 receptors...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116619/phylogenetic-analysis-and-molecular-typing-of-trichothecene-producing-fusarium-fungi-from-russian-collections
#3
A A Stakheev, L V Samokhvalova, O D Mikityuk, S K Zavriev
We performed a three-locus phylogenetic analysis of Fusarium strains presumably capable of trichothecene production, which were deposited in the Russian national collections. The intra- and interspecific polymorphism of partial sequences of the translation elongation factor 1 alpha ( TEF1α ) gene and two genes from the trichothecene cluster TRI5 and TRI14 was studied. A study of 60 strains of different origins using DNA markers confirmed, and in the case for several strains, clarified their taxonomic characteristics...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116618/soluble-guanylate-cyclase-as-the-key-enzyme-in-the-modulating-effect-of-no-on-metabotropic-glutamate-receptors
#4
I V Ryzhova, A D Nozdrachev, T V Tobias, E A Vershinina
The synaptic plasticity of the afferent synapse of the vestibular apparatus is defined by the dynamic interaction of ionotropic and metabotropic glutamate receptors and the modulators of synaptic transmission. It was shown that nitric oxide modulates iGluR responses. In this paper, the effect of NO on the function of the afferent synapse mGluR was investigated. Inhibitor of nitric oxide synthase lowered the level of background activity but increased the amplitude of the responses of groups I and II mGluR agonist ACPD...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116617/reversible-cyclic-thermal-inactivation-of-oligopeptidase-b-from-serratia-proteamaculans
#5
M V Ovchinnikova, A G Mikhailova, D M Karlinsky, V A Gorlenko, L D Rumsh
A unique property was found for oligopeptidase B from Serratia proteamaculans (PSP) as well as its mutants: they can undergo reversible thermal inactivation at 37°C, with activity being restored or even increased with respect to the initial one upon subsequent cooling. The process can be repeated several times, with the same results achieved (up to 5 cycles). This effect can be explained by a shift in the equilibrium between the inactive open form of the enzyme and the active closed one upon variation of the incubation temperature...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116616/1-4-phenoxybenzyl-5-aminouracil-derivatives-and-their-analogues-novel-inhibitors-of-human-adenovirus-replication
#6
N A Nikitenko, E S Gureeva, A A Ozerov, A I Tukhvatulin, F M Izhaeva, V S Prassolov, P G Deryabin, M S Novikov, D Y Logunov
Adenovirus infections are characterized by widespread distribution. The lack of causal therapy, which is effective in treating this group of diseases, explains the need for new therapeutic drugs. Notably, anti-adenoviral activity of [4-(phenoxy)benzyl]-5-(phenylamino)-6-azauracil, 1-[4-(phenoxy)benzyl]-5-(morpholino) uracil, 1-[4-(4-chlorophenoxy)benzyl]-5-(morpholino) uracil, and 1-[4-(4-fluorophenoxy)-benzyl]-5-(morpholino) uracil was observed.
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116615/characterization-of-the-t-cell-repertoire-after-autologous-hsct-in-patients-with-ankylosing-spondylitis
#7
E A Komech, I V Zvyagin, M V Pogorelyy, I Z Mamedov, D A Fedorenko, Y B Lebedev
Autologous hematopoietic stem cell transplantation (HSCT), a safer type of HSCT than allogeneic HSCT, is a promising therapy for patients with severe autoimmune diseases (ADs). Despite the long history of medical practice, structural changes in the adaptive immune system as a result of autologous HSCT in patients with various types of ADs remain poorly understood. In this study, we used high-throughput sequencing to investigate the structural changes in the peripheral blood T-cell repertoire in adult patients with ankylosing spondylitis (AS) during two years after autologous HSCT...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116614/cognitive-tagging-of-neurons-cremediated-genetic-labeling-and-characterization-of-the-cells-involved-in-learning-and-memory
#8
O I Ivashkina, N S Vorobyeva, A M Gruzdeva, M A Roshchina, K A Toropova, K V Anokhin
In this study, we describe use of Cre-mediated recombination to obtain a permanent genetic labeling of the brain neuronal networks activated during a new experience in animals. This method utilizes bitransgenic Fos-Cre-eGFP mice in which a green fluorescent protein is expressed upon tamoxifen-induced Cre-recombination only in the cells where immediate early gene c-fos expression takes place due to the new experience. We used the classical fear conditioning model to show that ex vivo microscopy of the eGFP protein in Fos-Cre-eGFP mice enables mapping of the neurons of the various brain regions that undergo Cre-recombination during acquisition of a new experience...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116613/the-development-and-study-of-recombinant-immunoglobulin-a-to-hemagglutinins-of-the-influenza-virus
#9
T K Aliev, I G Dement'yeva, V A Toporova, V V Argentova, L P Pozdnyakova, M N Bokov, Yu A Votchitseva, D A Dolgikh, S D Varfolomeyev, P G Sveshnikov, M P Kirpichnikov
We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins of the influenza A virus. On the basis of a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for the expression of the heavy and light chains of the immunoglobulins of IgA1-, IgA2m1-, and IgG-isotypes. Following transfection and selection, stable Chinese hamster ovary (CHO) cell lines were produced. The antibodies of IgA1-, IgA2m1-, and IgG-isotypes were purified from culture media. We performed an immunochemical characterization and studied their interactions with influenza A strains of the H1N1- and H3N2-subtypes...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116612/organism-level-tumor-models-in-zebrafish-danio-rerio
#10
I V Mizgirev, D R Safina, I V Demidyuk, S V Kostrov
Development and implementation of adequate organism-level models is one of the key elements in biomedical research that focuses on experimental oncology. Over the last decade, studies using Zebrafish ( Danio rerio ) have gained in popularity in this area of research. This review describes the various approaches that have been used in developing highly effective models for oncological (clinical term, better cancer or tumor) studies based on D. rerio . Priority is given to transplantation models of cancer and their application to optically transparent D...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116611/molecular-approaches-to-safe-and-controlled-engineered-t-cell-therapy
#11
R S Kalinin, A V Petukhov, V D Knorre, M A Maschan, A V Stepanov, A G Gabibov
Chimeric antigen receptor-modified T-cell therapy (CAR-T therapy) is one of the fastest developing areas of immuno-oncology. Over the past decade, it has revolutionized the cell therapy modality and expedited its pace of development, from optimization of the structure of chimeric antigen receptors and animal model experiments to successful clinical application. The initial designs of the CAR configuration focused on increasing T-cell activation, cytotoxicity, and persistence. However, the first attempts to treat patients with CAR T cells have demonstrated the need for increased safety and controlled activation of genetically modified T cells...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116610/hypertension-and-cerebral-microangiopathy-cerebral-small-vessel-disease-genetic-and-epigenetic-aspects-of-their-relationship
#12
L A Dobrynina, M R Zabitova, L A Kalashnikova, E V Gnedovskaya, M A Piradov
Hypertension (HT) and its cerebral complications are extremely vexing medical and social problems. Despite the obvious association between hypertension and the clinical and neuroimaging features of cerebral microangiopathy (CMA) (also known as cerebral small vessel disease), the causal links between them remain ambiguous. Besides, antihypertensive therapy as the only way to manage these patients does not always prevent brain damage. Knowledge about the key factors and mechanisms involved in HT and CMA development is important for predicting the risk of cerebral complications and developing new approaches to their prevention and treatment...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713522/influence-of-the-linking-order-of-fragments-of-ha2-and-m2e-of-the-influenza-a-virus-to-flagellin-on-the-properties-of-recombinant-proteins
#13
L A Stepanova, R Y Kotlyarov, M A Shuklina, E A Blochina, M V Sergeeva, M V Potapchuk, A A Kovaleva, N V Ravin, L M Tsybalova
The ectodomain of the M2 protein (M2e) and the conserved fragment of the second subunit of hemagglutinin (HA2) are promising candidates for broadly protective vaccines. In this paper, we report on the design of chimeric constructs with differing orders of linkage of four tandem copies of M2e and the conserved fragment of HA2 (76-130) from phylogenetic group II influenza A viruses to the C-terminus of flagellin. The 3D-structure of two chimeric proteins showed that interior location of the M2e tandem copies (Flg-4M2e-HA2) provides partial α-helix formation nontypical of native M2e on the virion surface...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713521/new-experimental-models-of-retinal-degeneration-for-screening-molecular-photochromic-ion-channel-blockers
#14
A Yu Rotov, L A Astakhova, V S Sitnikova, A A Evdokimov, V M Boitsov, M V Dubina, M N Ryazantsev, M L Firsov
Application of molecular photochromic ion channel blockers to recover the visual function of a degenerated retina is one of the promising trends in photopharmacology. To this day, several photochromic azobenzene-based compounds have been proposed and their functionality has been demonstrated on cell lines and knockout mouse models. Further advance necessitates testing of the physiological activity of a great number of new compounds. The goal of this study is to propose animal models of photoreceptor degeneration that are easier to obtain than knockout mouse models but include the main features required for testing the physiological activity of molecular photoswitches...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713520/gene-expression-in-the-three-spined-stickleback-gasterosteus-aculeatus-of-marine-and-freshwater-ecotypes
#15
S M Rastorguev, A V Nedoluzhko, N M Gruzdeva, E S Boulygina, S V Tsygankova, D Y Oshchepkov, A M Mazur, E B Prokhortchouk, K G Skryabin
Three-spine stickleback (Gasterosteus aculeatus) is a well-known model organism that is routinely used to explore microevolution processes and speciation, and the number of studies related to this fish has been growing recently. The main reason for the increased interest is the processes of freshwater adaptation taking place in natural populations of this species. Freshwater three-spined stickleback populations form when marine water three-spined sticklebacks fish start spending their entire lifecycle in freshwater lakes and streams...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713519/a-highly-productive-cho-cell-line-secreting-human-blood-clotting-factor-ix
#16
S V Kovnir, N A Orlova, M I Shakhparonov, K G Skryabin, A G Gabibov, I I Vorobiev
Hemophilia B patients suffer from an inherited blood-clotting defect and require regular administration of blood-clotting factor IX replacement therapy. Recombinant human factor IX produced in cultured CHO cells is nearly identical to natural, plasma-derived factor IX and is widely used in clinical practice. Development of a biosimilar recombinant human factor IX for medical applications requires the generation of a clonal cell line with the highest specific productivity possible and a high level of specific procoagulant activity of the secreted factor IX...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713518/the-ptenp1-pseudogene-unlike-the-pten-gene-is-methylated-in-normal-endometrium-as-well-as-in-endometrial-hyperplasias-and-carcinomas-in-middle-aged-and-elderly-females
#17
T F Kovalenko, K V Morozova, L A Ozolinya, I A Lapina, L I Patrushev
The tumor suppressor PTEN controls multiple cellular functions, including cell cycle, apoptosis, senescence, transcription, and mRNA translation of numerous genes. In tumor cells, PTEN is frequently inactivated by genetic mutations and epimutations. The aim of this study was to investigate the methylation patterns of the PTEN gene and its pseudogene PTENP1 as potential genetic markers of endometrial hyperplasia (EH) and endometrial carcinoma (EC). Methylation of the 5'-terminal regions of the PTEN and PTENP1 sequences was studied using methyl-sensitive PCR of genomic DNA isolated from 57 cancer, 43 endometrial hyperplasia, and normal tissue samples of 24 females aged 17-34 years and 19 females aged 45-65 years, as well as 20 peripheral venous blood samples of EC patients...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713517/the-effect-of-tnf-and-vegf-on-the-properties-of-ea-hy926-endothelial-cells-in-a-model-of-multi-cellular-spheroids
#18
S Sh Gapizov, L E Petrovskaya, L N Shingarova, E V Svirschevskaya, D A Dolgikh, M P Kirpichnikov
Endothelial cells play a major role in the development of inflammation and neoangiogenesis in cancer and chronic inflammatory diseases. In 3D cultures, cells are under conditions that closely resemble those existing in healthy and disease-stricken human organs and tissues. Therefore, the development of a 3D model based on the Ea.hy926 endothelial cell line is an urgent need in molecular and cellular biology. Cell cultivation on an anti-adhesive substrate under static conditions was shown to lead to the formation of spheroids (3D cultures)...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713516/pathogenesis-of-type-1-diabetes-mellitus-and-rodent-experimental-models
#19
I G Gvazava, O S Rogovaya, M A Borisov, E A Vorotelyak, A V Vasiliev
The global prevalence of diabetes mellitus and its severe complications is on the rise. The study of the pathogenesis of the onset and the progression of complications related to the disease, as well as the search for new therapeutic agents and methods of treatment, remains relevant. Experimental models are extremely important in the study of diabetes. This survey contains a synthesis of the most commonly used experimental animal models described in scientific literature. The mechanisms of the streptozotocin model are also analyzed and discussed, as it is considered as the most adequate and easily reproducible diabetes model...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713515/the-role-of-tal1-in-hematopoiesis-and-leukemogenesis
#20
E R Vagapova, P V Spirin, T D Lebedev, V S Prassolov
TAL1 (SCL/TAL1, T-cell acute leukemia protein 1) is a transcription factor that is involved in the process of hematopoiesis and leukemogenesis. It participates in blood cell formation, forms mesoderm in early embryogenesis, and regulates hematopoiesis in adult organisms. TAL1 is essential in maintaining the multipotency of hematopoietic stem cells (HSC) and keeping them in quiescence (stage G0). TAL1 forms complexes with various transcription factors, regulating hematopoiesis (E2A/HEB, GATA1-3, LMO1-2, Ldb1, ETO2 , RUNX1, ERG, FLI1)...
January 2018: Acta Naturae
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