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Acta Naturae

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https://www.readbyqxmd.com/read/29713522/influence-of-the-linking-order-of-fragments-of-ha2-and-m2e-of-the-influenza-a-virus-to-flagellin-on-the-properties-of-recombinant-proteins
#1
L A Stepanova, R Y Kotlyarov, M A Shuklina, E A Blochina, M V Sergeeva, M V Potapchuk, A A Kovaleva, N V Ravin, L M Tsybalova
The ectodomain of the M2 protein (M2e) and the conserved fragment of the second subunit of hemagglutinin (HA2) are promising candidates for broadly protective vaccines. In this paper, we report on the design of chimeric constructs with differing orders of linkage of four tandem copies of M2e and the conserved fragment of HA2 (76-130) from phylogenetic group II influenza A viruses to the C-terminus of flagellin. The 3D-structure of two chimeric proteins showed that interior location of the M2e tandem copies (Flg-4M2e-HA2) provides partial α-helix formation nontypical of native M2e on the virion surface...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713521/new-experimental-models-of-retinal-degeneration-for-screening-molecular-photochromic-ion-channel-blockers
#2
A Yu Rotov, L A Astakhova, V S Sitnikova, A A Evdokimov, V M Boitsov, M V Dubina, M N Ryazantsev, M L Firsov
Application of molecular photochromic ion channel blockers to recover the visual function of a degenerated retina is one of the promising trends in photopharmacology. To this day, several photochromic azobenzene-based compounds have been proposed and their functionality has been demonstrated on cell lines and knockout mouse models. Further advance necessitates testing of the physiological activity of a great number of new compounds. The goal of this study is to propose animal models of photoreceptor degeneration that are easier to obtain than knockout mouse models but include the main features required for testing the physiological activity of molecular photoswitches...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713520/gene-expression-in-the-three-spined-stickleback-gasterosteus-aculeatus-of-marine-and-freshwater-ecotypes
#3
S M Rastorguev, A V Nedoluzhko, N M Gruzdeva, E S Boulygina, S V Tsygankova, D Y Oshchepkov, A M Mazur, E B Prokhortchouk, K G Skryabin
Three-spine stickleback (Gasterosteus aculeatus) is a well-known model organism that is routinely used to explore microevolution processes and speciation, and the number of studies related to this fish has been growing recently. The main reason for the increased interest is the processes of freshwater adaptation taking place in natural populations of this species. Freshwater three-spined stickleback populations form when marine water three-spined sticklebacks fish start spending their entire lifecycle in freshwater lakes and streams...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713519/a-highly-productive-cho-cell-line-secreting-human-blood-clotting-factor-ix
#4
S V Kovnir, N A Orlova, M I Shakhparonov, K G Skryabin, A G Gabibov, I I Vorobiev
Hemophilia B patients suffer from an inherited blood-clotting defect and require regular administration of blood-clotting factor IX replacement therapy. Recombinant human factor IX produced in cultured CHO cells is nearly identical to natural, plasma-derived factor IX and is widely used in clinical practice. Development of a biosimilar recombinant human factor IX for medical applications requires the generation of a clonal cell line with the highest specific productivity possible and a high level of specific procoagulant activity of the secreted factor IX...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713518/the-ptenp1-pseudogene-unlike-the-pten-gene-is-methylated-in-normal-endometrium-as-well-as-in-endometrial-hyperplasias-and-carcinomas-in-middle-aged-and-elderly-females
#5
T F Kovalenko, K V Morozova, L A Ozolinya, I A Lapina, L I Patrushev
The tumor suppressor PTEN controls multiple cellular functions, including cell cycle, apoptosis, senescence, transcription, and mRNA translation of numerous genes. In tumor cells, PTEN is frequently inactivated by genetic mutations and epimutations. The aim of this study was to investigate the methylation patterns of the PTEN gene and its pseudogene PTENP1 as potential genetic markers of endometrial hyperplasia (EH) and endometrial carcinoma (EC). Methylation of the 5'-terminal regions of the PTEN and PTENP1 sequences was studied using methyl-sensitive PCR of genomic DNA isolated from 57 cancer, 43 endometrial hyperplasia, and normal tissue samples of 24 females aged 17-34 years and 19 females aged 45-65 years, as well as 20 peripheral venous blood samples of EC patients...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713517/the-effect-of-tnf-and-vegf-on-the-properties-of-ea-hy926-endothelial-cells-in-a-model-of-multi-cellular-spheroids
#6
S Sh Gapizov, L E Petrovskaya, L N Shingarova, E V Svirschevskaya, D A Dolgikh, M P Kirpichnikov
Endothelial cells play a major role in the development of inflammation and neoangiogenesis in cancer and chronic inflammatory diseases. In 3D cultures, cells are under conditions that closely resemble those existing in healthy and disease-stricken human organs and tissues. Therefore, the development of a 3D model based on the Ea.hy926 endothelial cell line is an urgent need in molecular and cellular biology. Cell cultivation on an anti-adhesive substrate under static conditions was shown to lead to the formation of spheroids (3D cultures)...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713516/pathogenesis-of-type-1-diabetes-mellitus-and-rodent-experimental-models
#7
I G Gvazava, O S Rogovaya, M A Borisov, E A Vorotelyak, A V Vasiliev
The global prevalence of diabetes mellitus and its severe complications is on the rise. The study of the pathogenesis of the onset and the progression of complications related to the disease, as well as the search for new therapeutic agents and methods of treatment, remains relevant. Experimental models are extremely important in the study of diabetes. This survey contains a synthesis of the most commonly used experimental animal models described in scientific literature. The mechanisms of the streptozotocin model are also analyzed and discussed, as it is considered as the most adequate and easily reproducible diabetes model...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713515/the-role-of-tal1-in-hematopoiesis-and-leukemogenesis
#8
E R Vagapova, P V Spirin, T D Lebedev, V S Prassolov
TAL1 (SCL/TAL1, T-cell acute leukemia protein 1) is a transcription factor that is involved in the process of hematopoiesis and leukemogenesis. It participates in blood cell formation, forms mesoderm in early embryogenesis, and regulates hematopoiesis in adult organisms. TAL1 is essential in maintaining the multipotency of hematopoietic stem cells (HSC) and keeping them in quiescence (stage G0). TAL1 forms complexes with various transcription factors, regulating hematopoiesis (E2A/HEB, GATA1-3, LMO1-2, Ldb1, ETO2 , RUNX1, ERG, FLI1)...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29713514/-social-life-of-senescent-cells-what-is-sasp-and-why-study-it
#9
A V Borodkina, P I Deryabin, A A Giukova, N N Nikolsky
Cellular senescence was first described as a failure of normal human cells to divide indefinitely in culture. Until recently, the emphasis in the study of cell senescence has been focused on the accompanying intracellular processes. The focus of the attention has been on the irreversible growth arrest and two important physiological functions that rely on it: suppression of carcinogenesis due to the proliferation loss of damaged cells, and the acceleration of organism aging due to the deterioration of the tissue repair mechanism with age...
January 2018: Acta Naturae
https://www.readbyqxmd.com/read/29340224/camkii-is-involved-in-the-choline-induced-downregulation-of-acetylcholine-release-in-mouse-motor-synapses
#10
A E Gaydukov, O P Balezina
We investigated the involvement of calcium-dependent enzymes, protein kinase C (PKC) and calcium-calmodulin-dependent protein kinase II (CaMKII), in the signaling pathway triggered by the activation of presynaptic alpha7-type nicotinic acetylcholine receptors by exogenous choline, leading to downregulation of the evoked acetylcholine (ACh) release in mouse motor synapses. Blockade of PKC with chelerythrine neither changed the evoked release of ACh by itself nor prevented the inhibitory effect of choline. The CaMKII blocker KN-62 did not affect synaptic activity but fully prevented the choline-induced downregulation of ACh release...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340223/yabby3-orthologous-genes-in-wild-tomato-species-structure-variability-and-expression
#11
M A Filyushin, M A Slugina, A V Shchennikova, E Z Kochieva
Evolution of the genes encoding YABBY transcription factors is believed to be one of the key reasons for flat leaf emergence from the radially symmetrical stem and gynoecium diversity. YABBY genes determine the identity of the abaxial surface of all aboveground lateral organs in seed plants. In the present study, complete sequences of YABBY3- orthologous genes were identified and characterized in 13 accessions of cultivated and wild tomato species with diverse morphophysiology of leaves, flowers, and fruits...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340222/direct-molecular-fishing-of-new-protein-partners-for-human-thromboxane-synthase
#12
A V Svirid, P V Ershov, E O Yablokov, L A Kaluzhskiy, Yu V Mezentsev, A V Florinskaya, T A Sushko, N V Strushkevich, A A Gilep, S A Usanov, A E Medvedev, A S Ivanov
Thromboxane synthase (TBXAS1) catalyzes the isomerization reaction of prostaglandin H2 producing thromboxane A2, the autocrine and paracrine factor in many cell types. A high activity and metastability by these arachidonic acid derivatives suggests the existence of supramolecular structures that are involved in the regulation of the biosynthesis and directed translocation of thromboxane to the receptor. The objective of this study was to identify TBXAS1 protein partners from human liver tissue lysate using a complex approach based on the direct molecular fishing technique, LC-MS/MS protein identification, and protein-protein interaction validation by surface plasmon resonance (SPR)...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340221/recombinant-antibodies-to-the-ebola-virus-glycoprotein
#13
A A Panina, I G Dementieva, T K Aliev, V A Toporova, D S Balabashin, M N Bokov, L P Pozdnyakova, O B Shchemchukova, D A Dolgikh, P G Sveshnikov, M P Kirpichnikov
Currently, there are no approved therapies for targeted prevention and treatment of Ebola hemorrhagic fever. In the present work, we describe the development of a eukaryotic expression system for the production of three full-length chimeric antibodies (IgG1-kappa isotypes) GPE118, GPE325, and GPE534 to the recombinant glycoprotein of the Ebola virus (EBOV GP), which is a key factor in the pathogenicity of the disease. The immunochemical properties of the obtained antibodies were studied by immunoblotting and indirect, direct, and competitive ELISA using the recombinant EBOV proteins rGPdTM, NP, and VP40...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340220/multilocus-analysis-of-genetic-susceptibility-to-myocardial-infarction-in-russians-replication-study
#14
N G Kukava, B V Titov, G J Osmak, N A Matveeva, O G Kulakova, A V Favorov, R M Shakhnovich, M Ya Ruda, O O Favorova
In search of genetic markers of myocardial infarction (MI) risk, which have prognostic significance for Russians, we performed a replication study of MI association with genetic variants of PCSK9 (rs562556), APOE (epsilon polymorphism, rs7412 and rs429358), LPL (rs320), MTHFR (rs1801133), eNOS (rs2070744), and the 9p21 region (rs1333049) in 405 patients with MI and 198 controls. Significant MI association was observed with variants of the lipid metabolism genes ( PCSK9 , APOE and LPL ), and of eNOS . The SNPs in the MTHFR gene and the 9p21 region were not significantly associated with MI one by one but were included in several different MI-associated allelic combinations identified by multilocus analysis...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340219/a-novel-hybrid-promoter-are-htert-for-cancer-gene-therapy
#15
S V Kalinichenko, M V Shepelev, P N Vikhreva, I V Korobko
describe a novel hybrid tumor-specific promoter, ARE-hTERT, composed of the human TERT gene promoter (hTERT) and the antioxidant response element (ARE) from the human GCLM gene promoter. The hybrid promoter retains the tumor specificity of the basal hTERT promoter but is characterized by an enhanced transcriptional activity in cancer cells with abnormal activation of the Nrf2 transcription factor and upon induction of oxidative stress. In the in vitro enzyme-prodrug cancer gene therapy scheme, ARE-hTERT promoter-driven expression of CD : UPRT (yeast cytosine deaminase : uracil phosphoribosyltransferase) chimeric protein induced a more pronounced death of cancer cells either upon treatment with 5-fluorouracil (5FC) alone or when 5FC was combined with chemotherapeutic drugs as compared to the hTERT promoter...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340218/synthesis-and-characterization-of-hybrid-core-shell-fe3o4-sio2-nanoparticles-for-biomedical-applications
#16
I V Zelepukin, V O Shipunova, A B Mirkasymov, P I Nikitin, M P Nikitin, S M Deyev
The creation of markers that provide both visual and quantitative information is of considerable importance for the mapping of tissue macrophages and other cells. We synthesized magnetic and magneto-fluorescent nanomarkers for the labeling of cells which can be detected with high sensitivity by the magnetic particle quantification (MPQ) technique. For stabilization under physiological conditions, the markers were coated with a dense silica shell. In this case, the size and zeta-potential of nanoparticles were controlled by a modified Stober reaction...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340217/translational-cross-activation-of-the-encapsidated-rna-of-potexviruses
#17
M V Arkhipenko, N A Nikitin, E K Donchenko, O V Karpova, J G Atabekov
We had shown the genomic RNA of potexviruses potato virus X and the alternanthera mosaic virus to be inaccessible in vitro to ribosomes while in intact virion form, but the RNAs can be translationally activated following the binding of movement protein 1 (MP1) to virus particles. Here, we present the results of the follow-up study targeting two more potexvirus species - the Narcissus mosaic virus and the Potato aucuba mosaic virus. We found encapsidated potexviral RNA to share common translational features in vitro and the MP1 to be potent over homological virions of its "own" species and over heterological virions of other species, as well exhibiting selective specificity...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340216/super-resolution-microscopy-in-studying-the-structure-and-function-of-the-cell-nucleus
#18
S S Ryabichko, A N Ibragimov, L A Lebedeva, E N Kozlov, Y V Shidlovskii
In recent decades, novel microscopic methods commonly referred to as super- resolution microscopy have been developed. These methods enable the visualization of a cell with a resolution of up to 10 nm. The application of these methods is of great interest in studying the structure and function of the cell nucleus. The review describes the main achievements in this field.
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340215/genome-stability-maintenance-in-naked-mole-rat
#19
I O Petruseva, A N Evdokimov, O I Lavrik
The naked mole-rat ( Heterocephalus glaber ) is one of the most promising models used to study genome maintenance systems, including the effective repair of damage to DNA. The naked mole-rat is the longest lived rodent species, which is extraordinarily resistant to cancer and has a number of other unique phenotypic traits. For at least 80% of its lifespan, this animal shows no signs of aging or any increased likelihood of death and retains the ability to reproduce. The naked mole-rat draws the heightened attention of researchers who study the molecular basis of lengthy lifespan and cancer resistance...
October 2017: Acta Naturae
https://www.readbyqxmd.com/read/29340214/the-contribution-of-ribosomal-protein-s1-to-the-structure-and-function-of-q%C3%AE-replicase
#20
Z S Kutlubaeva, H V Chetverina, A B Chetverin
The high resolution crystal structure of bacterial ribosome was determined more than 10 years ago; however, it contains no information on the structure of the largest ribosomal protein, S1. This unusual protein comprises six flexibly linked domains; therefore, it lacks a fixed structure and this prevents the formation of crystals. Besides being a component of the ribosome, protein S1 also serves as one of the four subunits of Qβ replicase, the RNA-directed RNA polymerase of bacteriophage Qβ. In each case, the role of this RNA-binding protein has been thought to consist in holding the template close to the active site of the enzyme...
October 2017: Acta Naturae
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