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Cold Spring Harbor Protocols

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https://www.readbyqxmd.com/read/28250233/elementary-protein-analysis-in-schizosaccharomyces-pombe
#1
Agnes Grallert, Iain M Hagan
Biochemical monitoring and interrogation of protein function is a critical component of most fission yeast studies. In particular, its small proteome size, high conservation of core molecular cell biology, and genetic malleability make Schizosaccharomyces pombe an excellent model organism in which to use mass spectrometry to conduct proteome-wide approaches. Here we discuss issues encountered during the analysis of fission yeast protein preparations.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250232/building-cell-structures-in-three-dimensions-electron-tomography-methods-for-budding-yeast
#2
Eileen T O'Toole, Thomas H Giddings, Mark Winey
Saccharomyces cerevisiae has been an important model system for numerous cellular, genetic, and molecular studies. However, this small eukaryote presents a challenge for imaging at the electron microscope level. Preparation of yeast using high-pressure freezing followed by freeze-substitution (HPF/FS) results in excellent preservation of cell structure in these difficult-to-fix samples. In particular, cells prepared by HPF/FS can be used for 3D electron tomography (ET) studies where optimum cell preservation is critical...
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250231/dmso-1-5-m-cryoprotectant-solution
#3
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250230/igg-wash-buffer
#4
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250229/modified-tap-lysis-buffer
#5
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250228/modified-tev-cleavage-buffer
#6
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250227/yeast-suspension-buffer
#7
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250226/bead-wash-buffer-d
#8
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250225/bead-wash-buffer-d
#9
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250224/bead-wash-buffer-c
#10
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250223/bead-wash-buffer-b
#11
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250222/bead-wash-buffer-a
#12
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250221/guanidine-hydrochloride-guhcl-buffer
#13
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250220/isothermal-reaction-gibson-assembly-master-mix
#14
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250219/iso-buffer-5%C3%A3
#15
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250218/low-salt-lb-solid-medium-containing-kanamycin-and-x-gal
#16
(no author information available yet)
No abstract text is available yet for this article.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250217/mouse-ovary-cryopreservation
#17
Jorge Sztein
Ovary cryopreservation in combination with orthotopic ovary transplantation can produce live offspring and complement embryo and sperm cryopreservation programs. One method for ovary cryopreservation is described here.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250216/mouse-ovary-transplantation
#18
Richard Behringer
Ovary transplantation is a useful technique when there is a valuable female mouse (e.g., transgenic, unique allele) that is ill, not breeding, or cannot breed naturally. The ovaries from the valuable female are transplanted into a histocompatible wild-type female recipient whose ovaries have been surgically removed. The donor ovaries can then function in the wild-type recipient to generate oocytes that will be ovulated and can be fertilized after natural matings. The donor ovaries can be cut into smaller pieces and each piece can be transplanted into separate females...
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250215/isolation-of-high-molecular-weight-dna-from-mammalian-tissues-using-proteinase-k-and-phenol
#19
Michael R Green, Joseph Sambrook
This procedure is the method of choice for purification of genomic DNA from mammalian tissues when large amounts of DNA are required, for example, for Southern blotting.
March 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28250214/improved-tandem-affinity-purification-tag-and-methods-for-isolation-of-proteins-and-protein-complexes-from-schizosaccharomyces-pombe
#20
Nicola Zilio, Michael N Boddy
The tandem affinity purification (TAP) method uses an epitope that contains two different affinity purification tags separated by a site-specific protease site to isolate a protein rapidly and easily. Proteins purified via the TAP tag are eluted under mild conditions, allowing them to be used for structural and biochemical analyses. The original TAP tag contains a calmodulin-binding peptide and the IgG-binding domain from protein A separated by a tobacco etch virus (TEV) protease cleavage site. After capturing the Protein A epitope on an IgG resin, bound proteins are released by incubation with the TEV protease and then isolated on a calmodulin matrix in the presence of calcium; elution from this resin is achieved by chelating calcium with EGTA...
March 1, 2017: Cold Spring Harbor Protocols
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