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Cold Spring Harbor Protocols

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https://www.readbyqxmd.com/read/28148885/stimulating-neurons-with-heterologously-expressed-light-gated-ion-channels
#1
J Simon Wiegert, Christine E Gee, Thomas G Oertner
Heterologous expression of ion channels that can be directly gated by light has made it possible to stimulate almost any excitable cell with light. Optogenetic stimulation has been particularly powerful in the neurosciences, as it allows the activation of specific, genetically defined neurons with precise timing. Organotypic hippocampal slice cultures are a favored preparation for optogenetic experiments. They can be cultured for many weeks and, after transfection with optogenetic actuators and sensors, allow the study of individual synapses or small networks...
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148884/prions
#2
Dmitry Kryndushkin, Herman K Edskes, Frank P Shewmaker, Reed B Wickner
Infectious proteins (prions) are usually self-templating filamentous protein polymers (amyloids). Yeast prions are genes composed of protein and, like the multiple alleles of DNA-based genes, can have an array of "variants," each a distinct self-propagating amyloid conformation. Like the lethal mammalian prions and amyloid diseases, yeast prions may be lethal, or only mildly detrimental, and show an array of phenotypes depending on the protein involved and the prion variant. Yeast prions are models for both rare mammalian prion diseases and for several very common amyloidoses such as Alzheimer's disease, type 2 diabetes, and Parkinson's disease...
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148883/k-gluconate-based-intracellular-solution
#3
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148882/slice-culture-transduction-solution
#4
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148881/slice-culture-medium
#5
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148880/dissection-solution
#6
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148879/tris-immunoprecipitation-buffer
#7
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148878/sodium-phosphate-immunoprecipitation-buffer
#8
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148877/hepes-immunoprecipitation-buffer
#9
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148876/na3vo4-stock-solution
#10
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148875/stop-buffer-for-cell-harvesting
#11
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148874/sds-loading-buffer-2%C3%A3
#12
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148873/protein-quantification-buffer
#13
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148872/yeast-extract-peptone-glycerol-ypg-medium
#14
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148871/half-strength-ypd-medium
#15
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148870/amyloid-prion-transfer-buffer
#16
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148869/modified-ripa-buffer-supplemented-with-nucleases
#17
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148868/amyloid-prion-resuspension-buffer
#18
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148867/amyloid-prion-buffer-r
#19
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28148866/yeast-disruption-buffer
#20
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2017: Cold Spring Harbor Protocols
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