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Cold Spring Harbor Protocols

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https://www.readbyqxmd.com/read/28373516/whole-cell-recording-in-the-awake-brain
#1
Doyun Lee, Albert K Lee
Intracellular recording is an essential technique for investigating cellular mechanisms underlying complex brain functions. Despite the high sensitivity of the technique to mechanical disturbances, intracellular recording has been applied to awake, behaving, and even freely moving, animals. Here we summarize recent advances in these methods and their application to the measurement and manipulation of membrane potential dynamics for understanding neuronal computations in behaving animals.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373515/mx-cassettes-for-knocking-out-genes-in-yeast
#2
John H McCusker
Precise modifications of the Saccharomyces cerevisiae genome use marker cassettes, most often in the form of "knockout" (KO) marker cassettes, to delete genes. Many different KO marker cassettes exist, some of which require strains with specific genotypes, such as auxotrophic mutations, and others that have no strain genotype requirements, such as selections for drug resistance and one of two selections for nitrogen source utilization. This introduction focuses on the most frequently used family of KO cassettes-the MX cassettes...
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373514/library-binding-buffer-2%C3%A3
#3
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373513/intracellular-pipette-solution
#4
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373512/polysomal-lysis-buffer-with-puromycin
#5
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373511/polysomal-lysis-buffer
#6
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373510/tes-buffer
#7
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373509/ypd-5-fluorocytosine-medium
#8
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373508/sdp-ura-5-foa-medium
#9
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373507/sd-ura-5-foa-medium
#10
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373506/sd-lys-%C3%AE-aminoadipate-medium
#11
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373505/sd-fluoroacetamide-medium
#12
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373504/sd-5-fluorocytosine-medium
#13
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373503/ypgal-medium
#14
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373502/ypd-liquid-medium-and-plates-with-drugs
#15
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373501/synthetic-dextrose-proline-sdp-plates-with-drugs
#16
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373500/synthetic-dextrose-glutamate-sde-plates-with-drugs
#17
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373499/synthetic-dextrose-sd-plates-without-ammonium-sulfate-or-amino-acids-with-or-without-acetamide-or-cytosine
#18
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373498/synthetic-dextrose-sd-plates-with-or-without-cytosine
#19
(no author information available yet)
No abstract text is available yet for this article.
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28373497/efficient-method-for-whole-cell-recording-in-freely-moving-rodents-using-ultraviolet-cured-collar-based-pipette-stabilization
#20
Doyun Lee, Albert K Lee
Whole-cell recording is a key technique for investigating synaptic and cellular mechanisms underlying various brain functions. However, because of its high sensitivity to mechanical disturbances, applying the whole-cell recording method to freely moving animals has been challenging. Here, we describe a technique for obtaining such recordings in freely moving, drug-free animals with a high success rate. This technique involves three major steps: obtaining a whole-cell recording from awake head-fixed animals, reliable and efficient stabilization of the pipette with respect to the animal's head using an ultraviolet (UV)-transparent collar and UV-cured adhesive, and rapid release of the animal from head fixation without loss of the recording...
April 3, 2017: Cold Spring Harbor Protocols
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