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Journal of Tropical Medicine

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https://www.readbyqxmd.com/read/28163725/development-and-validation-of-a-pcr-elisa-for-the-diagnosis-of-symptomatic-and-asymptomatic-infection-by-leishmania-leishmania-infantum
#1
Fernanda Alvarenga Cardoso Medeiros, Luciana Inácia Gomes, Edward Oliveira, Carolina Senra Alves de Souza, Maria Vitória Mourão, Gláucia Fernandes Cota, Letícia Helena Dos Santos Marques, Mariângela Carneiro, Ana Rabello
A kDNA PCR enzyme-linked immunosorbent assay (kDNA PCR-ELISA) for the diagnosis of human visceral leishmaniasis (HVL) was developed. The detection limit of the reaction, precision measurements, and cut-off of the kDNA PCR-ELISA were defined in a proof-of-concept phase. A reference strain of Leishmania (Leishmania) infantum and a bank of 14 peripheral blood samples from immunocompetent patients with VL were characterized using techniques considered gold standards, and 11 blood samples obtained from healthy individuals of an endemic area were also assessed...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28115947/molecular-epidemiology-and-genetic-diversity-of-norovirus-in-young-children-in-phnom-penh-cambodia
#2
Kaewkanya Nakjarung, Ladaporn Bodhidatta, Pimmnapar Neesanant, Paphavee Lertsethtakarn, Orntipa Sethabutr, Ket Vansith, Chhour Y Meng, Brett E Swierczewski, Carl J Mason
This study investigated the genetic diversity of noroviruses identified from a previous surveillance study conducted at the National Pediatric Hospital in Phnom Penh, Cambodia, from 2004 to 2006. In the previous study, 926 stool samples were collected from children aged 3-60 months with acute diarrhea (cases) and without diarrhea (controls) with reported 6.7% of cases and 3.2% of controls being positive for norovirus. The initial norovirus diagnostic assay was performed with real-time reverse transcription-polymerase chain reaction (real-time RT PCR) which also distinguished between genogroups I and II (GI and GII)...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28115946/potential-distribution-of-chagas-disease-vectors-hemiptera-reduviidae-triatominae-in-colombia-based-on-ecological-niche-modeling
#3
Gabriel Parra-Henao, Laura C Suárez-Escudero, Sebastián González-Caro
Ecological niche modeling of Triatominae bugs allow us to establish the local risk of transmission of the parasite Trypanosoma cruzi, which causes Chagas disease. This information could help to guide health authority recommendations on infection monitoring, prevention, and control. In this study, we estimated the geographic distribution of triatomine species in Colombia and identified the relationship between landscape structure and climatic factors influencing their occurrence. A total of 2451 records of 4 triatomine species (Panstrongylus geniculatus, Rhodnius pallescens, R...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28096819/urinary-schistosomiasis-among-children-in-murbai-and-surbai-communities-of-ardo-kola-local-government-area-taraba-state-nigeria
#4
R S Houmsou, H Agere, B E Wama, J B Bingbeng, E U Amuta, S L Kela
Background. This cross-sectional study was conducted to determine the prevalence, intensity of infection, and risk factors associated with urinary schistosomiasis among children in Murbai and Surbai communities of Ardo-Kola Local Government Area (LGA), Taraba State, Nigeria. Methods. Urine samples were analysed by the standard filtration technique using 10 ml syringe, Swinnex polypropylene filter holder (13 mm diameter), and polycarbonate membrane filters (12 μm porosity). Sociodemographic data and water contact activities were collated from children using structured questionnaires...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28096818/associated-factors-to-seroprevalence-of-ehrlichia-spp-in-dogs-of-quintana-roo-mexico
#5
Pedro Pablo Martínez-Vega, Manuel Emilio Bolio-Gonzalez, Roger Iván Rodríguez-Vivas, Eduardo Gutierrez-Blanco, Carlos Pérez-Osorio, Sandra Luz Villegas-Perez, Carlos Humberto Sauri-Arceo
The objective of this study was to determine the seroprevalence to Ehrlichia spp. in dogs from Xcalak, Quintana Roo, Mexico, and the associated factors. Serum samples were obtained from 118 dogs and used in an indirect immunofluorescent assay test for the detection of antibodies against Ehrlichia spp. A questionnaire was used to obtain information about possible variables associated with seroprevalence. These variables were analyzed through Chi(2) test and logistic regression. Dog seroprevalence of antibodies against Ehrlichia spp...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28050172/lipid-profile-of-children-with-malaria-by-plasmodium-vivax
#6
Rosa Maria Dias, Jose Luiz Fernandes Vieira, Bianca da Conceição Cabral, Isameriliam Rosaulem Pereira da Silva, Laelia Maria Barra Feio Brasil, Eliete da Cunha Araújo, Marcieni Ataíde de Andrade
Background. Changes in lipid profile are commonly reported in adult patients with malaria. However, a few studies evaluated lipid abnormalities in children continuously exposed to P. vivax. Objective. To evaluate lipid abnormalities in children with P. vivax infection and to assess if parasite count or the history of malaria correlates with lipid levels at admission. Methods. A total of 75 children were included in the study, from which 43 were slide confirmed infection by P. vivax. Serial blood samples were collected at admission and, on days 7 and 14, evaluated for the colorimetric measurements of triglycerides, very low-density lipoprotein (VLDL), total cholesterol, high-density lipoprotein (HDL), and low-density lipoprotein (LDL)...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28050171/current-epidemiological-assessment-of-bancroftian-filariasis-in-tanga-region-northeastern-tanzania
#7
Happyness J Mshana, Vito Baraka, Gerald Misinzo, Williams H Makunde
Background. Tanzania started a countrywide lymphatic filariasis elimination programme in 2000 adopting the mass drug administration (MDA) strategy. The drug used for the programme was the combination of ivermectin and albendazole. However, there is limited information on the current epidemiological trend of the infections, where MDA implementation is ongoing. The present study aimed at assessing the current status of Bancroftian filariasis infection rate and morbidity where MDA has been distributed and administered for over eight rounds...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28042300/phlebotomines-diptera-psychodidae-in-a-hydroelectric-system-affected-area-from-northern-amazonian-brazil-further-insights-into-the-effects-of-environmental-changes-on-vector-ecology
#8
Nercy Virginia Rabelo Furtado, Allan Kardec Ribeiro Galardo, Clicia Denis Galardo, Viviane Caetano Firmino, Thiago Vasconcelos Dos Santos
During 2012-2015, an entomological survey was conducted as part of a phlebotomine (Diptera: Psychodidae) monitoring program in an area influenced by the Santo Antônio do Jari hydroelectric system (Amapá State, Brazil). The purpose was to study aspects of Amazon/Guianan American cutaneous leishmaniasis (ACL) vectors subjected to stresses by anthropogenic environmental changes. For sampling, CDC light traps were positioned 0.5, 1, and 20 m above ground at five capture locations along the Jari River Basin...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28042299/antimalarial-potential-of-carica-papaya-and-vernonia-amygdalina-in-mice-infected-with-plasmodium-berghei
#9
Oche Okpe, Nathan Habila, Joseph Ikwebe, Vincent A Upev, Stanley I R Okoduwa, Omiagocho T Isaac
The study determined if administration of Vernonia amygdalina and Carica papaya plants provides synergistic effects in ameliorating plasmodium infection in mice. Thirty mice (17.88-25.3 g) were divided into 6 groups of 5 mice each. Group 1 was normal control, while groups 2-6 were intraperitoneally inoculated 2.5 × 10(7)Plasmodium berghei parasitized red blood cell, followed by daily administration of 350 mg/kg aqueous leaf extracts after establishment of infection. Group 2 was disease control, while group 6 was treated with standard drug for four consecutive days...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28025597/clinical-profiles-of-dengue-infection-during-an-outbreak-in-northern-india
#10
Anish Laul, Poonam Laul, Vamsi Merugumala, Ravi Pathak, Urvashi Miglani, Pinkee Saxena
Introduction. Dengue fever is an arboviral disease, which is transmitted by mosquito vector and presents as varied clinical spectrum of dengue fever (DF), dengue hemorrhagic fever (DHF), dengue shock syndrome (DSS), and expanded dengue syndrome (EDS) with atypical presentations, thus posing a diagnostic dilemma. Unless we are aware of these presentations, diagnosis as well as early initiation of treatment becomes difficult. We studied the various clinical presentations of dengue infection during an outbreak of disease in 2015...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27956905/molecular-characterization-of-cryptosporidium-spp-in-wild-rodents-of-southwestern-iran-using-18s-rrna-gene-nested-pcr-rflp-and-sequencing-techniques
#11
Jasem Saki, Masoud Foroutan-Rad, Reza Asadpouri
Background. Rodents could act as reservoir for Cryptosporidium spp. specially C. parvum, a zoonotic agent responsible for human infections. Since there is no information about Cryptosporidium infection in rodents of Ahvaz city, southwest of Iran, hence, this survey was performed to determine the prevalence and molecular characterization of Cryptosporidium spp. in this region. Materials and Methods. One hundred rodents were trapped from different regions of Ahvaz city. Intestine contents and fecal specimens of rodents were studied using both microscopy examination to identify oocyst and nested-polymerase chain reaction (PCR) technique for 18s rRNA gene detection...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27956904/antibacterial-action-of-curcumin-against-staphylococcus-aureus-a-brief-review
#12
REVIEW
Sin-Yeang Teow, Kitson Liew, Syed A Ali, Alan Soo-Beng Khoo, Suat-Cheng Peh
Curcumin, the major constituent of Curcuma longa L. (Zingiberaceae family) or turmeric, commonly used for cooking in Asian cuisine, is known to possess a broad range of pharmacological properties at relatively nontoxic doses. Curcumin is found to be effective against Staphylococcus aureus (S. aureus). As demonstrated by in vitro experiment, curcumin exerts even more potent effects when used in combination with various other antibacterial agents. Hence, curcumin which is a natural product derived from plant is believed to have profound medicinal benefits and could be potentially developed into a naturally derived antibiotic in the future...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27891154/preparation-of-ecofriendly-formulations-containing-biologically-active-monoterpenes-with-their-fumigant-and-residual-toxicities-against-adults-of-culex-pipiens
#13
Mohamed E I Badawy, Nehad E M Taktak, Osama M Awad, Souraya A Elfiki, Nadia E Abou El-Ela
Different mixtures of monoterpenes (ketone, alcohol, and alkene) were loaded on paper discs and wax and their knockdown activities were evaluated against Culex pipiens adults. Some individual monoterpenes were also evaluated by residual toxicity technique. Citronella oil as a reference was also loaded separately or in combination with monoterpenes on paper discs and wax. The ketone monoterpenes mixture (camphor, menthone, carvone, and fenchone) on paper discs was the most active (KT50 = 17.20 min) followed by ketone monoterpenes with citronella oil (KT50 = 20...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27872647/antimalarial-properties-of-aqueous-crude-extracts-of-gynostemma-pentaphyllum-and-moringa-oleifera-leaves-in-combination-with-artesunate-in-plasmodium-berghei-infected-mice
#14
Voravuth Somsak, Preeyanuch Borkaew, Chokdee Klubsri, Kittiyaporn Dondee, Panatda Bootprom, Butsarat Saiphet
Due to the emergence and spread of malaria parasite with resistance to antimalarial drugs, discovery and development of new, safe, and affordable antimalarial are urgently needed. In this respect, medicinal plant extracts are targets to optimize antimalarial actions and restore efficacy of standard antimalarial drugs. The present study was aimed at determining the antimalarial activities of Gynostemma pentaphyllum and Moringa oleifera leaf extracts in combination with artesunate against Plasmodium berghei-infected mice...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27829843/health-care-seeking-behavior-of-persons-with-acute-chagas-disease-in-rural-argentina-a-qualitative-view
#15
Ignacio Llovet, Graciela Dinardi, Cecilia Canevari, Nahal Torabi
Chagas disease (CD) is a tropical parasitic disease largely underdiagnosed and mostly asymptomatic affecting marginalized rural populations. Argentina regularly reports acute cases of CD, mostly young individuals under 14 years old. There is a void of knowledge of health care seeking behavior in subjects experiencing a CD acute condition. Early treatment of the acute case is crucial to limit subsequent development of disease. The article explores how the health outcome of persons with acute CD may be conditioned by their health care seeking behavior...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27829842/the-role-of-anethum-graveolens-l-dill-in-the-management-of-diabetes
#16
REVIEW
Mohammad Taghi Goodarzi, Iraj Khodadadi, Heidar Tavilani, Ebrahim Abbasi Oshaghi
Aim. There is evidence that Anethum graveolens (AG) has been used for centuries in Asian traditional medicine, and its constituents have useful effects on the control and management of diabetes and cardiovascular disorders. AG has many useful effects, including hypolipidemic and hypoglycemic effects, and it has been reported to reduce the incidence of diabetic complications. It acts mainly by affecting antioxidant capacity and change in some genes in glucose and lipid pathways. The aim of the present paper was to summarize pharmacological effects of AG in the management of diabetes...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27807452/child-survival-strategies-assessment-of-knowledge-and-practice-of-rural-women-of-reproductive-age-in-cross-river-state-nigeria
#17
Aniekan Jumbo Etokidem, Ofonime Johnson
Introduction. Nigeria is one of the five countries that account for about 50% of under-five mortality in the world. The objective of this study was to assess the knowledge and practice of child survival strategies among rural community caregivers in Cross River State of Nigeria. Materials and Methods. This descriptive cross-sectional survey used a pretested questionnaire to obtain information from 150 women of reproductive age. Data analysis was done using SPSS version 20. Results. The child survival strategy known to most of the respondents was oral rehydration therapy as indicated by 98% followed by female education by 73...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27738439/electrocardiographic-study-in-adult-homozygous-sickle-cell-disease-patients-in-lagos-nigeria
#18
Adedoyin Dosunmu, Akinsegun Akinbami, Ebele Uche, Adewumi Adediran, Sarah John-Olabode
Background. This study sought to identify the pattern of electrocardiographic changes in steady state adult sickle cell anaemia. Methods. A case-control, cross-sectional study was conducted amongst sickle cell patients attending the sickle cell clinic of Lagos State University Teaching Hospital, Ikeja, and HbAA controls. All consenting participants had haemoglobin electrophoresis done and were subjected to electrocardiography (ECG). The descriptive data were given as means ± standard deviation (SD). The differences were considered to be statistically significant when the p value obtained was <0...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27651801/pattern-clinical-characteristics-and-outcome-of-meningitis-among-hiv-infected-adults-admitted-in-a-tertiary-hospital-in-north-western-tanzania-a-cross-sectional-study
#19
Matobogolo M Boaz, Samuel Kalluvya, Jennifer A Downs, Bonaventura C T Mpondo, Stephen E Mshana
Background. Limited information exists on the etiologies, clinical characteristics, and outcomes of meningitis among HIV-infected patients in Africa. We conducted a study to determine the etiology, clinical characteristics, and outcomes of meningitis among HIV-infected adults. Methods. A prospective cross-sectional hospital based study was conducted among HIV-infected patients aged ≥18 years admitted to the medical wards with symptoms and signs of meningitis. Sociodemographic and clinical information were collected using a standardized data collection tool...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27635146/detection-of-urinary-tract-pathology-in-some-schistosoma-haematobium-infected-nigerian-adults
#20
O S Onile, H O Awobode, V S Oladele, A M Agunloye, C I Anumudu
Screening for Schistosoma haematobium infection and its possible morbidity was carried out in 257 adult participants in Eggua community, Ogun State, Nigeria. Parasitological assessment for the presence of ova of S. haematobium in urine and abdominopelvic ultrasonographic examination for bladder and secondary kidney pathology were carried out. S. haematobium prevalence of 25.68% (66/257) was recorded among the participants. There was a significantly higher prevalence of 69.2% of urinary schistosomiasis in the females than the prevalence of 31...
2016: Journal of Tropical Medicine
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