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Journal of Tropical Medicine

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https://www.readbyqxmd.com/read/29681951/lateral-flow-loop-mediated-isothermal-amplification-test-with-stem-primers-detection-of-cryptosporidium-species-in-kenyan-children-presenting-with-diarrhea
#1
Timothy S Mamba, Cecilia K Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu, Zablon K Njiru
Background. Cryptosporidium is a protozoan parasite and a major cause of diarrhea in children and immunocompromised patients. Current diagnostic methods for cryptosporidiosis such as microscopy have low sensitivity while techniques such as PCR indicate higher sensitivity levels but are seldom used in developing countries due to their associated cost. A loop-mediated isothermal amplification (LAMP) technique, a method with shorter time to result and with equal or higher sensitivity compared to PCR, has been developed and applied in the detection of Cryptosporidium species...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29666658/phylogenetic-analysis-of-dengue-virus-in-bangkalan-madura-island-east-java-province-indonesia
#2
Teguh Hari Sucipto, Tomohiro Kotaki, Kris Cahyo Mulyatno, Siti Churrotin, Amaliah Labiqah, Soegeng Soegijanto, Masanori Kameoka
Dengue virus (DENV) infection is a major health issue in tropical and subtropical areas. Indonesia is one of the biggest dengue endemic countries in the world. In the present study, the phylogenetic analysis of DENV in Bangkalan, Madura Island, Indonesia, was performed in order to obtain a clearer understanding of its dynamics in this country. A total of 359 blood samples from dengue-suspected patients were collected between 2012 and 2014. Serotyping was conducted using a multiplex Reverse Transcriptase-Polymerase Chain Reaction and a phylogenetic analysis of E gene sequences was performed using the Bayesian Markov chain Monte Carlo (MCMC) method...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29666657/the-effects-of-aloe-vera-cream-on-the-expression-of-cd4-and-cd8-lymphocytes-in-skin-wound-healing
#3
Yos Adi Prakoso, Kurniasih
The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150-200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29666656/hepatitis-b-virus-infection-in-tanzania-current-status-and-challenges
#4
REVIEW
Semvua B Kilonzo, Daniel W Gunda, Bonaventura C T Mpondo, Fatma A Bakshi, Hyasinta Jaka
Hepatitis B is one of the most common infectious diseases in the world with high prevalence in most of sub-Saharan Africa countries. The complexity in its diagnosis and treatment poses a significant management challenge in the resource-limited settings including Tanzania, where most of the tests and drugs are either unavailable or unaffordable. This mini review aims at demonstrating the current status of the disease in the country and discussing the concomitant challenges in diagnosis, treatment, and prevention...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29666655/determinants-of-chronic-respiratory-symptoms-among-pharmaceutical-factory-workers
#5
Sahle Asfaw, Fikre Enquselassie, Yifokire Tefera, Muluken Gizaw, Samson Wakuma, Messay Woldemariam
Background: Chronic respiratory symptoms including chronic cough, chronic phlegm, wheezing, shortness of breath, and chest pain are manifestations of respiratory problems which are mainly evolved as a result of occupational exposures. This study aims to assess determinants of chronic respiratory symptoms among pharmaceutical factory workers. Methods: A case control study was carried out among 453 pharmaceutical factory workers with 151 cases and 302 controls. Data was collected using pretested and structured questionnaire...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29666654/determinants-of-antenatal-healthcare-utilisation-by-pregnant-women-in-third-trimester-in-peri-urban-ghana
#6
Jones Asafo Akowuah, Peter Agyei-Baffour, Dadson Awunyo-Vitor
Access to quality healthcare still remains a major challenge in the efforts at reversing maternal morbidity and mortality. Despite the availability of established maternal health interventions, the health of the expectant mother and the unborn child remains poor due to low utilisation of interventions. The study examined the socioeconomic determinants of antenatal care utilisation in peri-urban Ghana using pregnant women who are in their third trimester. Two-stage sampling technique was used to sample 200 pregnant women who were in their third trimester from the District Health Information Management System software...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29623095/understanding-unlicensed-drug-vendor-practices-related-to-childhood-malaria-in-one-rural-district-of-uganda-an-exploratory-study
#7
Eric Liow, Rosemin Kassam, Richard Sekiwunga
This study investigated unlicensed drug outlets' practices for the management of malaria in the rural district of Butaleja, Uganda. A qualitative design using semistructured interviews was used. Interviews were recorded, translated, transcribed, and analyzed using thematic analysis. A total of 75 vendors, representing 85% of the outlets in the study area, were interviewed. Most of the vendors were associated with a drug shop type of outfit. About three-quarters reported having completed some level of postsecondary education, but just one-tenth of the vendors had qualifications that made them eligible to apply for a license to operate a drug shop...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29623094/health-related-quality-of-life-among-tb-patients-question-mark-on-performance-of-tb-dots-in-pakistan
#8
Madeeha Malik, Rida Nasir, Azhar Hussain
Due to long duration of treatment and use of several agents, tuberculosis can lead to poor health related quality of life among patients. Objective. The present study was designed to assess health related quality of life among pulmonary tuberculosis patients in Pakistan. Methodology. A descriptive cross-sectional study design was used. SF-36 was self-administered to a sample of 382 pulmonary tuberculosis patients receiving self-administered or directly observed types of treatment, in baseline, initial, or continuous phase of treatment...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29552040/repellent-activity-of-trig-n-n-diethyl-benzamide-against-man-biting-mosquitoes
#9
Shandala Msangi, Eliningaya Kweka, Aneth Mahande
A study was conducted to assess efficacy of a new repellent brand TRIG (15% N-N Diethyl Benzamide) when compared to DEET (20% N-N Methyl Toluamide). The repellents were tested in laboratory and field. In the laboratory, the repellence was tested on human volunteers, by exposing their repellent-treated arms on starved mosquitoes in cages for 3 minutes at hourly intervals, while counting the landing and probing attempts. Anopheles gambiae and Aedes aegypti mosquitoes were used. Field evaluation was conducted by Human Landing Catch technique...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29410683/knowledge-and-acceptability-of-human-papillomavirus-vaccination-among-women-attending-the-gynaecological-outpatient-clinics-of-a-university-teaching-hospital-in-lagos-nigeria
#10
Kehinde S Okunade, Oyebola Sunmonu, Gbemisola E Osanyin, Ayodeji A Oluwole
Objectives: This study was aimed at determining the knowledge and acceptability of HPV vaccine among women attending the gynaecology clinics of the Lagos University Teaching Hospital (LUTH). Methods: This was a descriptive cross-sectional study involving 148 consecutively selected women attending the gynaecology clinic of LUTH. Relevant information was obtained from these women using an interviewer-administered questionnaire. The data was analysed and then presented by simple descriptive statistics using tables and charts...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29379526/performance-evaluation-of-commercial-dengue-diagnostic-tests-for-early-detection-of-dengue-in-clinical-samples
#11
Tuan Nur Akmalina Mat Jusoh, Rafidah Hanim Shueb
The shattering rise in dengue virus infections globally has created a need for an accurate and validated rapid diagnostic test for this virus. Rapid diagnostic test (RDT) and reverse transcription-polymerase chain reaction (RT-PCR) diagnostic detection are useful tools for diagnosis of early dengue infection. We prospectively evaluated the diagnostic performance of nonstructural 1 (NS1) RDT and real-time RT-PCR diagnostic kits in 86 patient serum samples. Thirty-six samples were positive for dengue NS1 antigen while the remaining 50 were negative when tested with enzyme-linked immunosorbent assay (ELISA)...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29358959/a-review-on-the-cosmeceutical-and-external-applications-of-nigella-sativa
#12
REVIEW
Ahmad M Eid, Nagib A Elmarzugi, Laila M Abu Ayyash, Maher N Sawafta, Hadeel I Daana
It is estimated by the World Health Organization (WHO) that most of the world's population depends on herbal medicine for their health care. Nigella sativa (N. sativa), also known as black-caraway and as "Kalonji," is a well-known seed all over the world. It is one of the most common medicinal plants worldwide and contains many useful chemical constituents that we can find in its fixed oil, such as thymoquinone, thymohydroquinone, dithymoquinone, thymol, nigellicine, carvacrol, nigellimine, nigellicine, nigellidine, and alpha-hederin...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29225629/synthesis-sar-and-docking-studies-disclose-2-arylfuran-1-4-naphthoquinones-as-in-vitro-antiplasmodial-hits
#13
Tatiane Freitas Borgati, Maria Fernanda Alves do Nascimento, Juma Fortunato Bernardino, Lunamaura Claudia Oliveira Martins, Alex Gutterres Taranto, Alaíde Braga de Oliveira
A total of 28 lapachol-related naphthoquinones with four different scaffolds were synthesized and spectroscopically characterized. In vitro antiplasmodial activity was assayed against the chloroquine-resistant Plasmodium falciparum W2 strain by the parasite lactate dehydrogenase ( p LDH) method. Cytotoxicity against Hep G2A16 cell was determined by the MTT assay. All compounds disclosed higher in vitro antiplasmodial activity than lapachol. Ortho- and para -naphthoquinones with a furan ring fused to the quinonoid moiety were more potent than 2-hydroxy-3-(1'-alkenyl)-1,4-naphthoquinones, while ortho -furanonaphthoquinones were more cytotoxic...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29209371/corrigendum-to-dengue-virus-ns1-protein-as-a-diagnostic-marker-commercially-available-elisa-and-comparison-to-qrt-pcr-and-serological-diagnostic-assays-currently-used-by-the-state-of-florida
#14
Jason H Ambrose, Shamala Devi Sekaran, Azliyati Azizan
[This corrects the article DOI: 10.1155/2017/8072491.].
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29181037/the-burden-and-trend-of-blood-borne-pathogens-among-asymptomatic-adult-population-in-akwatia-a-retrospective-study-at-the-st-dominic-hospital-ghana
#15
Sylvester Yao Lokpo, Mavis Popuelle Dakorah, Gameli Kwame Norgbe, James Osei-Yeboah, Godwin Adzakpah, Isaac Sarsah, John Gameli Deku, Innocent Afeke, Emmanuel Akomanin Asiamah, Nana Yaw Barimah Manaphraim, Isaac Asare, Bright Justice Ayidzoe, Emmanuel Alote Allotey, Emmanuel Agbeko Nani, Paul Amoah
Background: This study was aimed at evaluating the seroprevalence and trend of blood-borne pathogens (HIV, HCV, HBV, and Syphilis) among asymptomatic adults at Akwatia during a four-year period (2013-2016). Materials and Methods: The study was a retrospective analysis of secondary data of blood donors who visited the hospital from January 2013 to December 2016. Archival data from 11,436 prospective donors was extracted. Data included age, sex, and place of residence as well as results of infectious markers (HIV, HBV, HCV, and Syphilis)...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29138642/observation-of-blood-donor-recipient-malaria-parasitaemia-patterns-in-a-malaria-endemic-region
#16
Jamilu Abdullahi Faruk, Gboye Olufemi Ogunrinde, Aisha Indo Mamman
Background: Asymptomatic malaria parasitaemia has been documented in donor blood in West Africa. However, donated blood is not routinely screened for malaria parasites (MPs). The present study therefore aimed to document the frequency of blood transfusion-induced donor-recipient malaria parasitaemia patterns, in children receiving blood transfusion in a tertiary health-centre. Methodology: A cross-sectional, observational study involving 140 children receiving blood transfusion was carried out...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29138641/naturally-acquired-antibody-responses-to-a-synthetic-malaria-antigen-as202-11
#17
Rebeka Nazareth, Pius Horumpende, Tolbert Sonda, Arnold Ndaro, Edson Mollel, Eliakim Paul, Emmanuel Athanase, Jaffu Chilongola
Background: A major challenge to malaria vaccine development is identification of protective epitopes and respective protective immune responses. Objective: To characterize naturally acquired Immunoglobulin G (IgG) responses to the synthetic peptide AS202.11, a malaria vaccine candidate. Methodology: This community based cross-sectional study enrolled 320 participants aged 1 year and above. Demographic information was recorded through interviews...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29138640/prevalence-of-soil-transmitted-helminthiases-and-schistosomiasis-in-preschool-age-children-in-mwea-division-kirinyaga-south-district-kirinyaga-county-and-their-potential-effect-on-physical-growth
#18
Stephen Sifuna Wefwafwa Sakari, Amos K Mbugua, Gerald M Mkoji
Intestinal parasitic infections can significantly contribute to the burden of disease, may cause nutritional and energetic stress, and negatively impact the quality of life in low income countries of the world. This cross-sectional study done in Mwea irrigation scheme, in Kirinyaga, central Kenya, assessed the public health significance of soil-transmitted helminthiases (STH), schistosomiasis, and other intestinal parasitic infections, among 361 preschool age children (PSAC) through fecal examination, by measuring anthropometric indices, and through their parents/guardians, by obtaining sociodemographic information...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29123551/discovery-of-antimalarial-drugs-from-streptomycetes-metabolites-using-a-metabolomic-approach
#19
REVIEW
Siti Junaidah Ahmad, Mohd Badrin Hanizam Abdul Rahim, Syarul Nataqain Baharum, Mohd Shukri Baba, Noraziah Mohamad Zin
Natural products continue to play an important role as a source of biologically active substances for the development of new drug. Streptomyces , Gram-positive bacteria which are widely distributed in nature, are one of the most popular sources of natural antibiotics. Recently, by using a bioassay-guided fractionation, an antimalarial compound, Gancidin-W, has been discovered from these bacteria. However, this classical method in identifying potentially novel bioactive compounds from the natural products requires considerable effort and is a time-consuming process...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28828009/liver-fibrosis-and-hepatitis-b-coinfection-among-art-na%C3%A3-ve-hiv-infected-patients-at-a-tertiary-level-hospital-in-northwestern-tanzania-a-cross-sectional-study
#20
Semvua B Kilonzo, Daniel W Gunda, Flora Kashasha, Bonaventura C Mpondo
BACKGROUND: Liver fibrosis which is a common complication of chronic hepatitis B infection is rarely diagnosed in low-resource countries due to limited capacity to perform biopsy studies. Data on the utilization of noninvasive techniques which are feasible for diagnosis of liver fibrosis in these settings among HIV-infected patients is scarce. The objective of this study was to establish the magnitude of liver fibrosis by using both aspartate-aminotransferase-to-platelets ratio and fibrosis-4 scores with associated hepatitis B coinfection among antiretroviral therapy naïve HIV-infected patients...
2017: Journal of Tropical Medicine
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