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Ticks and Tick-borne Diseases

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https://www.readbyqxmd.com/read/27908773/does-the-effect-of-a-metarhizium-anisopliae-isolate-on-rhipicephalus-microplus-depend-on-the-tick-population-evaluated
#1
Anelise Webster, Endrigo Pradel, Ugo Araújo Souza, João Ricardo Martins, José Reck, Augusto Schrank, Guilherme Klafke
The filamentous fungus Metarhizium anisopliae is an arthropod pathogen used in the biological control of pests in agriculture and livestock. The effect of the fungus M. anisopliae on ticks has been shown in experiments in vitro. The aim of the present study was to compare the susceptibility of different field isolates of Rhipicephalus microplus to M. anisopliae. A total of 67 field isolates were evaluated. Rhipicephalus microplus larvae were immersed in an M. anisopliae suspension (10(8) conidia/mL) for 5min...
November 24, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27894863/molecular-evidence-for-toxoplasma-gondii-in-feeding-and-questing-ixodes-ricinus-ticks
#2
Małgorzata Adamska, Bogumiła Skotarczak
The aim of the present study was to detect Toxoplasma gondii in ticks collected from ponies and field vegetation and to determine the role of Shetland ponies as a potential reservoir host for T. gondii. A total of 1737 feeding Ixodes ricinus collected from 49 horses and 371 questing ticks were tested by PCR and sequencing for the presence and genotyping of T. gondii. All ticks were examined in a previous study to detect and identify pathogenic bacterial species. The aim of this study was also to detect co-infection of ticks with these bacteria and T...
November 21, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27884572/tick-borne-encephalitis-virus-neutralization-by-high-dose-intravenous-immunoglobulin
#3
Jana Elsterova, Martin Palus, Jana Sirmarova, Jan Kopecky, Hans Helmut Niller, Daniel Ruzek
Tick-borne encephalitis (TBE) is a potentially lethal neuroinfection in humans, caused by TBE virus (TBEV). Currently, there are no approved therapeutic agents to treat TBE. Previously, it was suggested that application of high dose intravenous immunoglobulin (IVIG) may pose potentially successful treatment for severe cases of TBE. In this study, we determined the titers of TBEV-neutralizing antibodies in two IVIG lots originating from the same manufacturer, and tested their ability to treat a lethal TBEV-infection in a mouse model...
November 18, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27908772/anaplasma-platys-in-dogs-from-uruguay
#4
Luis Carvalho, Maria Teresa Armua-Fernandez, Nicolás Sosa, María Laura Félix, José Manuel Venzal
Anaplasmataceae family members include vector-borne bacteria of veterinary importance that may also affect humans. Ehrlichia canis and Anaplasma platys are the main members of this family detected in dogs worldwide. In Uruguay there are not many published studies on tick-borne pathogens affecting dogs, the only haemoparasite molecularly confirmed in dogs, is the piroplasm Rangelia vitalii. The aim of the present work was to detect the presence of A. platys and E. canis in dogs and dogs-associated ticks of two localities in Northwestern Uruguay...
November 17, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27908771/molecular-detection-and-genetic-characterization-of-babesia-theileria-and-anaplasma-amongst-apparently-healthy-sheep-and-goats-in-the-central-region-of-turkey
#5
Mo Zhou, Shinuo Cao, Ferda Sevinc, Mutlu Sevinc, Onur Ceylan, Sepil Ekici, Charoonluk Jirapattharasate, Paul Franck Adjou Moumouni, Mingming Liu, Guanbo Wang, Aiko Iguchi, Patrick Vudriko, Hiroshi Suzuki, Xuenan Xuan
Babesia spp., Theileria spp. and Anaplasma spp. are significant tick-borne pathogens of livestock globally. In this study, we investigated the presence and distribution of Babesia ovis, Theileria ovis and Anaplasma ovis in 343 small ruminants (249 sheep and 94 goats) from 13 towns in the Central Anatolia region of Turkey using species-specific PCR assays. The PCR were conducted using the primers based on the B. ovis ssu rRNA (BoSSUrRNA), T. ovis ssu rRNA (ToSSUrRNA) and A. ovis major surface protein 4 (AoMSP4) genes, respectively...
November 16, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27856176/identification-of-i-ricinus-i-persulcatus-and-i-trianguliceps-species-by-multiplex-pcr
#6
Kairi Värv, Anna Ivanova, Julia Geller, Jaanus Remm, Kertu Jaik, Nina Tikunova, Vera Rar, Åke Lundkvist, Irina Golovljova
Correct identification of tick species is an essential requirement for any scientific study engaged in tick-associated research. However, morphological identification can lead to misinterpretations, especially when dealing with vector-host research and sub-adult, engorged or damaged specimens. To overcome this limitation, we developed a novel assay to discriminate between Ixodes ricinus, I. persulcatus and I. trianguliceps species collected from rodents or vegetation, using the second internal transcribed spacer (ITS2) as a genetic marker...
November 10, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27838290/duration-of-borrelia-mayonii-infectivity-in-an-experimental-mouse-model-for-feeding-ixodes-scapularis-larvae
#7
Marc C Dolan, Nicole E Breuner, Andrias Hojgaard, J Charles Hoxmeier, Mark A Pilgard, Adam J Replogle, Lars Eisen
A novel species within the Borrelia burgdorferi sensu lato complex, Borrelia mayonii, was recently described and found to be associated with Lyme borreliosis in the Upper Midwest of the United States. The blacklegged tick, Ixodes scapularis, is naturally infected with B. mayonii in the Upper Midwest and has been experimentally demonstrated to serve as a vector for this spirochete. Natural vertebrate reservoirs for B. mayonii remain unknown. In this study, we demonstrate that an experimental spirochete host, the CD-1 strain outbred white mouse, can maintain active infection with B...
November 5, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27838291/surveillance-for-ixodes-ricinus-ticks-acari-ixodidae-on-the-faroe-islands
#8
Jolyon M Medlock, Kayleigh Hansford, Alexander G C Vaux, William Simonsen, Jens-Kjeld Jensen, Christina Joensen, Veerle Versteirt, Leivur Janus Hansen
Ixodes ricinus ticks are expanding their geographic range in Europe, both latitudinally in Scandinavia, and altitudinally in the European Alps. This paper details the findings of both passive and active surveillance on the Faroe Islands. Active field surveillance, using tick dragging, was conducted at 38 sites across the main seven inhabited islands of the Faroes during June-August 2015. Field sampling was conducted at all wooded sites on the islands of Vágar, Streymoy, Eysturoy, Borðoy, Kunoy and Suðuroy as well as in urban parks in the capital Tórshavn, among seabird colonies and at a bird observatory on Nólsoy, at moorland sites on Vágar and Borðoy, and a coastal headland on Suðuroy...
November 4, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27825733/a-comparison-of-dna-extraction-protocols-from-blood-spotted-on-fta-cards-for-the-detection-of-tick-borne-pathogens-by-reverse-line-blot-hybridization
#9
Zerihun Hailemariam, Jabbar Sabir Ahmed, Peter-Henning Clausen, Ard Menzo Nijhof
An essential step in the molecular detection of tick-borne pathogens (TBPs) in blood is the extraction of DNA. When cooled storage of blood under field conditions prior to DNA extraction in a dedicated laboratory is not possible, the storage of blood on filter paper forms a promising alternative. We evaluated six DNA extraction methods from blood spotted on FTA Classic(®) cards (FTA cards), to determine the optimal protocol for the subsequent molecular detection of TBPs by PCR and the Reverse Line Blot hybridization assay (RLB)...
November 3, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27825659/ferritin-1-silencing-effect-in-rhipicephalus-sanguineus-sensu-lato-acari-ixodidae-during-experimental-infection-with-ehrlichia-canis
#10
Joana Ferrolho, Sandra Antunes, Gustavo S Sanches, Joana Couto, Patrícia M Évora, Catarina Rosa, Marcos R André, Rosângela Z Machado, Gervásio H Bechara, Ana Domingos
Rhipicephalus sanguineus sensu lato (s.l.) is a very common ectoparasite of domestic dogs able to transmit several pathogens of human and veterinary importance. Tick infestations and tick-borne diseases (TBDs) remain a serious and persistent problem, due to the lack of efficient control measures. It is therefore vital that novel approaches to control are pursued. Whilst vaccination is recognised as a potential control method to reduce tick infestation, no anti-R. sanguineus vaccine is available. Ticks depend on their blood meals to obtain nutrients and to achieve sexual maturity, which exposes them to vast amounts of iron...
October 29, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27825658/tick-acari-ixodidae-infestations-in-cattle-along-geba-river-basin-in-guinea-bissau
#11
Sara Tudela Zúquete, João Coelho, Fernanda Rosa, Yolanda Vaz, Bernardo Cassamá, Ludovina Padre, Dulce Santos, Afonso P Basto, Alexandre Leitão
Tick infestations are a major problem for animal production in tropical areas where prevention and control remain deficient. The present study sought to assess the awareness of traditional cattle producers towards the importance of ticks and aimed at the identification of tick species infesting bovines within the Geba River basin, Guinea-Bissau. Interviews with producers revealed that the majority directly correlates the presence of ticks with the occurrence of diseases in cattle. However, insufficient or inadequate control approaches prevail...
October 29, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27818182/novel-phlebovirus-detected-in-haemaphysalis-parva-ticks-in-a-greek-island
#12
Anna Papa, Anastasia Kontana, Katerina Tsioka, Anastasios Saratsis, Smaragda Sotiraki
During the last decade the number of novel tick-borne phleboviruses has increased rapidly, especially after the identification of severe fever with thrombocytopenia syndrome and Heartland viruses which can cause severe disease in humans. A novel virus, Antigone virus was recently detected in ticks collected from the mainland of Greece. The aim of the present study was to investigate the presence of tick-borne phleboviruses in an island in Greece. During November 2015, 31 ticks were collected from sheep in Lesvos island...
October 29, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27802919/the-microbiome-of-neotropical-ticks-parasitizing-on-passerine-migratory-birds
#13
Khemraj Budachetri, Jaclyn Williams, Nabanita Mukherjee, Michael Sellers, Frank Moore, Shahid Karim
Seasonal migration of passerine birds between temperate North America and tropical Central and South America is an ecological phenomenon. Migration of birds has been associated with the introduction of ectoparasites like ticks or tick-borne pathogens across the avian migration routes. In this study, the microbial diversity was determined in the ticks and bird DNA samples using 454 pyrosequencing of bacterial 16S rRNA gene. Tick DNA samples showed the dominance of genera Lactococcus, Francisella, Raoultella, Wolbachia and Rickettsia across all the ticks, but birds DNA did not share common microbial diversity with ticks...
October 28, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27793613/comparative-biology-of-the-tropical-and-temperate-species-of-rhipicephalus-sanguineus-sensu-lato-acari-ixodidae-under-different-laboratory-conditions
#14
Marcelo B Labruna, Monize Gerardi, Felipe S Krawczak, Jonas Moraes-Filho
Recent studies have shown that the taxon Rhipicephalus sanguineus sensu lato (s.l.) is represented in Latin America by two distinct species, designated as 'tropical species' (distributed from Mexico to Brazil) and 'temperate species' (restricted to the southern cone of South America). Since both tropical and temperate species of R. sanguineus s.l. are parasites primarily of domestic dogs, the reasons for their distinct geographical distribution in South America could be related to particular requirements of abiotic conditions for off-host development...
October 24, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27793612/high-seroprevalence-for-indigenous-spotted-fever-group-rickettsiae-in-forestry-workers-from-the-federal-state-of-brandenburg-eastern-germany
#15
Silke Wölfel, Stephanie Speck, Sandra Essbauer, Bryan R Thoma, Marc Mertens, Sandra Werdermann, Olaf Niederstrasser, Eckhardt Petri, Rainer G Ulrich, Roman Wölfel, Gerhard Dobler
In the last decade six Rickettsia species, including Rickettsia slovaca have been characterized in Germany. All of these species could be linked to distinct clinical syndromes in humans. However, due to lack of seroepidemiological data an estimation of the prevalence and the public health impact of rickettsial infections in Germany is difficult. The aim of the present study was to determine the seroprevalence of spotted fever group (SFG) rickettsiae in a population with an elevated exposure risk to ticks. For that purpose, 559 sera of forestry workers in the federal state of Brandenburg, Eastern Germany, were screened for SFG-rickettsiae reactive IgG antibodies...
October 22, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27789159/seroprevalence-of-babesia-bovis-b-bigemina-trypanosoma-evansi-and-anaplasma-marginale-antibodies-in-cattle-in-southern-egypt
#16
Ragab M Fereig, Samy G A Mohamed, Hassan Y A H Mahmoud, Mahmoud Rezk AbouLaila, Azirwan Guswanto, Thu-Thuy Nguyen, Adel Elsayed Ahmed Mohamed, Noboru Inoue, Ikuo Igarashi, Yoshifumi Nishikawa
Babesia bovis, B. bigemina, Trypanosoma evansi, and Anaplasma marginale infections cause serious diseases in cattle, and are primarily transmitted by arthropod vectors (ticks for B. bovis, B. bigemina, and A. marginale and various types of flies for T. evansi). In the last few years, there have been many reports of a high prevalence of certain protozoan infections in northern Egypt, but no accurate or adequate data are available for the southern regions. Therefore, in this study, we screened for evidence of such diseases in economically important cattle species using serum samples...
October 20, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27769656/putative-rna-viral-sequences-detected-in-an-ixodes-scapularis-derived-cell-line
#17
Ryo Nakao, Keita Matsuno, Yongjin Qiu, Junki Maruyama, Nao Eguchi, Naganori Nao, Masahiro Kajihara, Kentaro Yoshii, Hirofumi Sawa, Ayato Takada, Chihiro Sugimoto
Ticks harbour various microorganisms, some of which act as pathogens of humans and animals. The recent advancement of genome sequencing technologies revealed that a wide range of previously unrecognised microorganisms exist in ticks. Continuous cell lines established from ticks could play a key role in the isolation of such microorganisms; however, tick cells themselves have been known to harbour symbiotic microorganisms. The present study aimed to characterise putative RNA viral sequences detected in the culture supernatant of one of the most frequently used tick cell lines, ISE6, which was derived from embryos of the blacklegged tick Ixodes scapularis...
October 15, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27769655/microorganisms-in-ticks-acari-ixodidae-collected-on-marsupials-and-rodents-from-santa-catarina-paran%C3%A3-and-mato-grosso-do-sul-states-brazil
#18
Carolina Moreira Blanco, Bernardo Rodrigues Teixeira, Alexandro Guterres da Silva, Renata Carvalho de Oliveira, Liana Strecht, Maria Ogrzewalska, Elba Regina S de Lemos
Information about tick fauna and monitoring of pathogen prevalences in ticks (Acari: Ixodidae) in various habitat types can enhance knowledge about the epidemiology of tick-borne pathogens in Brazil. This work shows the results of a study of tick parasitism of wild rodents and marsupials collected in seven localities in the southern part of Brazil, within Atlantic Forest and Cerrado biomes. A total of 61 ticks were collected from small mammals, and after identification to the species level, the ticks were individually tested for the presence of bacteria of the genera Rickettsia, Borrelia, family Anaplasmataceae, and protozoa of the genus Babesia...
October 15, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27773826/risk-of-encountering-ticks-and-tick-borne-pathogens-in-a-rapidly-growing-metropolitan-area-in-the-u-s-great-plains
#19
Bruce H Noden, Scott R Loss, Courtney Maichak, Faithful Williams
The prevalence of tick-borne diseases has increased dramatically in many urban areas of the U.S., yet little is known about the ecology of ticks and tick-borne pathogens in relation to characteristics of North American urban and suburban landscapes. This study aimed to begin identification of the risk of encountering ticks and tick-borne pathogens within a rapidly expanding metropolitan area in the U.S. Great Plains region. Ten sites across Oklahoma City, Oklahoma were selected for tick sampling based on presence of tick habitat and level of urbanization intensity...
October 14, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/27771333/ixodid-ticks-in-the-megapolis-of-kyiv-ukraine
#20
Artem S Rogovskyy, Igor V Nebogatkin, Glen A Scoles
The Ixodidae include the most common tick species encountered in Europe. The ticks transmit a variety of bacterial and protozoan agents of medical and veterinary significance. The aim of the current work was to investigate distribution of Ixodes ricinus and Dermacentor reticulatus ticks in Kyiv, the largest and most densely populated megapolis of Ukraine. Ticks were collected at various recreational areas by flagging during May, the month that showed the highest tick abundance in the past. Sex distribution among I...
October 14, 2016: Ticks and Tick-borne Diseases
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