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Ticks and Tick-borne Diseases

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https://www.readbyqxmd.com/read/28927817/development-and-evaluation-of-a-two-step-multiplex-taqman-real-time-pcr-assay-for-detection-quantification-of-different-genospecies-of-borrelia-burgdorferi-sensu-lato
#1
Mónica Nunes, Ricardo Parreira, Teresa Carreira, João Inácio, Maria Luísa Vieira
Nowadays, at least four clinically important B. burgdorferi sensu lato (s.l.) genospecies (B. afzelii, B. garinii, B. burgdorferi sensu stricto (s.s.) and B. lusitaniae) circulate in Portugal. Each genospecies has a different tropism that resuls in a diverse array of clinical manifestations. The standard diagnostic procedure used is normally simple, nevertheless, during the "window-period" phase, in which specific antibodies cannot yet be detected, diagnosis becomes difficult, and calls for reliable, sensitive and specific laboratory methods, such as molecular tests...
September 11, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28919405/exploration-of-stable-isotope-analysis-for-tick-host-identification
#2
Kathleen LoGiudice, Kathryn Kurchena, Katherine Christopher, Natasha Scott
Due to the problem of tick-borne diseases, there is a need to better understand the importance of different host species in maintaining enzootic disease cycles. We explored the utility of stable isotope analysis to identify the larval hosts of questing ixodid ticks. In laboratory experiments, we used Ixodes scapularis and two host species that are important in the Lyme disease system in eastern North America. First, we tested how effectively a short-term dietary tracer ((13)C in corn) was reflected in molted ticks...
September 7, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28899663/prevalence-of-antibodies-against-severe-fever-with-thrombocytopenia-syndrome-virus-in-shelter-dogs-in-the-republic-of-korea
#3
Seung-Hun Lee, Hyun-Joo Kim, Min-Jung Lee, Jae-Won Byun, Da-Young Kim, Neung-Hee Kim, Doo-Hwan Kim, Dongmi Kwak, Hae-Eun Kang, Hyang-Mi Nam
The purpose of this study was to investigate the seroprevalence of antibodies against severe fever with thrombocytopenia syndrome virus (SFTSV) in shelter dogs in the Republic of Korea (ROK) using an indirect immunofluorescence assay and virus neutralization test. Sera were collected from 426 dogs in 12 animal shelters throughout the ROK from March 2016 to November 2016. Overall, 59 of 426 (13.9%) samples were seropositive for antibodies against SFTSV. A significant difference was observed in accordance with the sampling region (p<0...
September 5, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28890111/tick-borne-pathogens-and-their-reservoir-hosts-in-northern-italy
#4
Ivana Baráková, Markéta Derdáková, Diana Selyemová, Michal Chvostáč, Eva Špitalská, Fausta Rosso, Margherita Collini, Roberto Rosà, Valentina Tagliapietra, Matteo Girardi, Claudio Ramponi, Heidi C Hauffe, Annapaola Rizzoli
The aim of this study was to determine the occurrence of Anaplasma phagocytophilum, Rickettsia spp., Babesia spp., and Candidatus Neoehrlichia mikurensis in Ixodes spp. ticks removed from wildlife, domestic animals and humans in the Province of Trento (northern Italy) in order to better understand their ecology and provide public health professionals with an updated list of pathogens which should be considered during their diagnostic procedures after a tick bite. During 2011-2012, 848 feeding ticks at all life stages (adults, nymphs and larvae) from various hosts (wild ungulates, birds and rodents; domestic sheep, dogs and humans) were collected...
September 1, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28887102/molecular-detection-of-vector-borne-pathogens-in-greek-cats
#5
Mathios E Mylonakis, Megan Schreeg, Manolis K Chatzis, Julian Pearce, Henry S Marr, Manolis N Saridomichelakis, Adam J Birkenheuer
Infectious diseases have been increasingly recognized in cats worldwide. The objective of this study was the molecular investigation of the prevalence of selected pathogens in healthy and sick cats from Greece, a country highly endemic for several canine vector-borne pathogens. Blood and/or bone marrow samples from 50 clinically healthy and 50 sick adult (>1 year-old) cats were retrospectively examined for the amplification of Bartonella spp., haemoplasmas, Ehrlichia spp., Anaplasma spp., Babesia spp., and Cytauxzoon spp...
September 1, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28882512/epidemiology-of-babesia-anaplasma-and-trypanosoma-species-using-a-new-expanded-reverse-line-blot-hybridization-assay
#6
Martina Soledad Paoletta, Ludmila López Arias, Sofía de la Fournière, Eliana Carolina Guillemi, Carlos Luciani, Néstor Fabián Sarmiento, Juan Mosqueda, Marisa Diana Farber, Silvina Elizabeth Wilkowsky
Vector-borne hemoparasitic infections are a major problem that affects livestock industries worldwide, particularly in tropical and subtropical regions. In this work, a reverse line blot (RLB) hybridization assay was developed for the simultaneous detection and identification of Anaplasma, Babesia and bovine trypanosomes, encompassing in this way the most relevant hemoparasites that affect cattle. A total of 186 bovine blood samples collected from two different ecoepidemiological regions of northeast Argentina, with and without tick control, were analyzed with this new RLB...
September 1, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28918352/report-of-the-19th-annual-meeting-of-the-international-scientific-working-group-on-tick-borne-encephalitis-isw-tbe-tbe-in-a-changing-world
#7
LETTER
Ursula Kunze
The 19th meeting of the International Scientific Working Group on Tick-Borne Encephalitis (ISW-TBE) - a group of neurologists, general practitioners, clinicians, travel physicians, virologists, pediatricians and epidemiologists-was held under the title "TBE in a changing world". Key topics within virology, current epidemiological developments and investigations, expansion of risk areas, clinical aspects and cases, traveling and mobility, vaccination rates, and latest news on vaccination were presented and extensively discussed...
August 30, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28869190/year-to-year-variation-in-the-density-of-ixodes-ricinus-ticks-and-the-prevalence-of-the-rodent-associated-human-pathogens-borrelia-afzelii-and-b-miyamotoi-in-different-forest-types
#8
Sanne C Ruyts, Wesley Tack, Evy Ampoorter, Elena C Coipan, Erik Matthysen, Dieter Heylen, Hein Sprong, Kris Verheyen
The human pathogens Borrelia afzelii, which causes Lyme borreliosis and B. miyamotoi, which causes relapsing fever, both circulate between Ixodes ricinus ticks and rodents. The spatiotemporal dynamics in the prevalence of these pathogens have not yet been fully elucidated, but probably depend on the spatiotemporal population dynamics of small rodents. We aimed to evaluate the effect of different forest types on the density of infected nymphs in different years and to obtain more knowledge about the spatial and temporal patterns of ticks and tick-borne pathogens...
August 26, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28887101/molecular-epidemiological-haematological-and-biochemical-evaluation-in-asymptomatic-theileria-annulata-infected-cattle-from-an-endemic-region-in-spain
#9
Lydia Calleja-Bueno, Ángel Sainz, Mercedes García-Sancho, Fernando Rodríguez-Franco, Juan V González-Martín, Alejandra Villaescusa
Mediterranean theileriosis is one of the most fatal theilerioses, with considerable economic impact on livestock production. The potential consequences of asymptomatic infection on the animal's health and on the epidemiology in endemic regions are still unclear. The objectives of this study were to determine the molecular prevalence of T. annulata in a representative population of asymptomatic cattle in extensive management in Madrid, Central Spain, an area where practitioners frequently report cases of clinical theileriosis, and to evaluate the existence of associations between infection by this pathogen and haematological, biochemical and epidemiological data...
August 25, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28887100/verification-of-post-chemotherapeutic-clearance-of-theileria-equi-through-concordance-of-nested-pcr-and-immunoblot
#10
L N Wise, L S Kappmeyer, M G Silva, S N White, J F Grause, D P Knowles
Certain countries including the United States remain non-endemic for particular infectious diseases such as equine piroplasmosis through import restrictions and surveillance. Endemic regions often employ premunition as the primary method to control disease, however in non-endemic countries, chemosterilization combined with methods to confirm parasite elimination are required to maintain disease-free status. The ability of imidocarb diproprionate (ID) to clear persistent Theileria equi infection from infected horses has been shown through the inability of treated horses to transmit via blood transfer...
August 25, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28865955/nanoparticles-as-effective-acaricides-against-ticks-a-review
#11
REVIEW
Giovanni Benelli, Filippo Maggi, Donato Romano, Cesare Stefanini, Baskaralingam Vaseeharan, Suresh Kumar, Akon Higuchi, Abdullah A Alarfaj, Heinz Mehlhorn, Angelo Canale
Ticks serve as vectors of a wide range of infectious agents deleterious to humans and animals. Tick bite prevention is based to a large extent on the use of chemical repellents and acaricides. However, development of resistance in targeted ticks, environmental pollution, and contamination of livestock meat and milk are major concerns. Recently, metal, metal oxide and carbon nanoparticles, particularly those obtained through green fabrication routes, were found to be highly effective against a wide array of arthropod pests and vectors...
August 25, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28869191/co-infection-with-babesia-divergens-and-anaplasma-phagocytophilum-in-cattle-bos-taurus-sweden
#12
Martin O Andersson, Bronislava Víchová, Conny Tolf, Sandra Krzyzanowska, Jonas Waldenström, Maria E Karlsson
Babesiosis is a severe disease in cattle worldwide. In Europe, the main causative agent of bovine babesiosis is Babesia divergens. In some areas, this species is reported to have declined or even disappeared, and its etiological role overtaken by other piroplasmid species. Moreover, co-infection with other tick-transmitted pathogens can be expected to complicate diagnosis in cattle. Hence, molecular identification of the causative agent of babesiosis should be a priority. Therefore, samples from 71 domestic cattle, 39 with clinical signs of babesiosis and 32 without, from southern Sweden were screened for Babesia spp...
August 24, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28847698/borrelia-burgdorferi-sensu-lato-in-ixodes-longiscutatus-ticks-from-brazilian-pampa
#13
Bruno Dall'Agnol, Thaís Michel, Bárbara Weck, Ugo Araújo Souza, Anelise Webster, Bruna Ferreira Leal, Guilherme Marcondes Klafke, João Ricardo Martins, Ricardo Ott, José Manuel Venzal, Carlos Alexandre Sanchez Ferreira, José Reck
Borrelia burgdorferi sensu lato (s.l.) complex includes the agents of Lyme disease/borreliosis in North America, Europe, and Asia, such Borrelia burgdorferi sensu stricto, Borrelia afzelii, Borrelia garinii, Borrelia bavariensis, Borrelia spielmanii, Borrelia bissettiae, and Borrelia mayonii. In 2013 B. burgdorferi s.l. was reported for the first time in the Neotropical region, from Ixodes aragaoi ticks in Uruguayan Pampa. In addition, from 2011 to 2016, 17 suspected human cases of borreliosis-like syndrome were reported in Rio Grande do Sul (RS) state, Brazil, which contains only part of country in the Pampa biome...
August 23, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28843481/prevalence-of-borrelia-miyamotoi-and-borrelia-burgdorferi-sensu-lato-in-questing-ticks-from-a-recreational-coniferous-forest-of-east-saxony-germany
#14
Sándor Szekeres, Jenny Lügner, Volker Fingerle, Gabriele Margos, Gábor Földvári
The hard tick Ixodes ricinus is the most important vector of tick-transmitted pathogens in Europe, frequently occurring in urban parks and greenbelts utilized for recreational activities. This species is the most common vector of the causative agents of Lyme borreliosis in Europe. Similarly, the species spreads Borrelia miyamotoi, causing a relapsing-fever like illness. A total of 1774 Ixodes ricinus (50 females, 68 males, 840 nymphs and 818 larvae) were collected with flagging between March and September 2014 in a coniferous forest patch in Niederkaina near the town of Bautzen in Saxony, Germany...
August 18, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28882513/enhanced-detection-of-rickettsia-species-in-ixodes-pacificus-using-highly-sensitive-fluorescence-in-situ-hybridization-coupled-with-tyramide-signal-amplification
#15
Ghazaleh Bagheri, Jeremy D Lehner, Jianmin Zhong
Ixodes pacificus is a host of many bacteria including Rickettsia species phylotypes G021 and G022. As part of the overall goal of understanding interactions of phylotypes with their tick host, this study focused on molecular detection of rickettsiae in ovarian and midgut tissue of I. pacificus by fluorescent in situ hybridization (FISH), PCR, and ultrastructural analysis. Of three embedding media (Technovit 8100, Unicryl, and paraffin) tested for generating thin sections, tissues embedded in paraffin resulted in the visualization of bacteria with low autofluorescence in FISH...
August 9, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28802807/acute-phase-response-in-babesia-canis-and-dirofilaria-immitis-co-infections-in-dogs
#16
Zorana Milanović, Anja Ilić, Jelena Francuski Andrić, Vladimir Radonjić, Anđelo Beletić, Milica Kovačević Filipović
Babesia canis and Dirofilaria immitis are emerging and geographically overlapping vector-borne pathogens in dogs. Infection with B. canis leads to acute-phase response (APR) that can be mild to severe and results in either non-complicated or complicated forms of the disease. The aim of this study was to determine whether acute B. canis infection is more severe in dogs with underlying asymptomatic D. immitis infection. Dogs of both sexes, different ages and breeds, with naturally occurring mono-infections with B...
August 2, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28784308/properties-of-the-tick-borne-encephalitis-virus-population-during-persistent-infection-of-ixodid-ticks-and-tick-cell-lines
#17
Oxana A Belova, Alexander G Litov, Ivan S Kholodilov, Liubov I Kozlovskaya, Lesley Bell-Sakyi, Lidiya Iu Romanova, Galina G Karganova
Tick-borne encephalitis virus (TBEV) is the causative agent of tick-borne encephalitis (TBE), a vector-borne zoonotic neuroinfection. For successful circulation in natural foci the virus has to survive in the vector for a long period of time. Information about the effect of long-term infection of ticks on properties of the viral population is of great importance. In recent years, changes in the eco-epidemiology of TBEV due to changes in distribution of ixodid ticks have been observed. These changes in TBEV-endemic areas could result in a shift of the main tick vector species, which in turn may lead to changes in properties of the virus...
July 25, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28774495/wild-and-domestic-animals-likely-involved-in-rickettsial-endemic-zones-of-northwestern-colombia
#18
Andrés F Londoño, Leidy Y Acevedo-Gutiérrez, Diana Marín, Verónica Contreras, Francisco J Díaz, Gustavo Valbuena, Marcelo B Labruna, Marylin Hidalgo, Margarita Arboleda, Salim Mattar, Sergio Solari, Juan D Rodas
Between 2006 and 2008, three outbreaks of human rickettsiosis occurred in Northwestern Colombia (municipalities of Necoclí, Los Córdobas and Turbo), with case fatality rates between 27% and 54%. The aim of this study was to determine previous exposure of wild and domestic animals to spotted fever group (SFG) rickettsiae through serological tests, to detect rickettsial evidence in their ectoparasites, and to analyze their possible role in the epidemiology of rickettsial diseases in this zone of the country...
July 21, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28736193/relapsing-fever-causative-agent-in-southern-iran-is-a-closely-related-species-to-east-african-borreliae
#19
Saied Reza Naddaf, Behnaz Ghazinezhad, Elham Kazemirad, Sally Jane Cutler
We obtained two blood samples from relapsing fever patients residing in Jask County, Hormozgan Province, southern Iran in 2013. Sequencing of a partial fragment of glpQ from two samples, and further characterization of one of them by analyzing flaB gene, and 16S-23S spacer (IGS) revealed the greatest sequence identity with East African borreliae, Borrelia recurrentis, and Borrelia duttonii, and Borrelia microti from Iran. Phylogenetic analyses of glpQ, flaB, and concatenated sequences (glpQ, flab, and IGS) clustered these sequences amongst East African Relapsing fever borreliae and B...
July 17, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28768603/tick-surveillance-for-borrelia-miyamotoi-and-phylogenetic-analysis-of-isolates-in-mongolia-and-japan
#20
Yukie Iwabu-Itoh, Boldbaatar Bazartseren, Oyunnomin Naranbaatar, Enkhmandakh Yondonjamts, Kiwa Furuno, Kyunglee Lee, Kozue Sato, Hiroki Kawabata, Nobuhiro Takada, Masako Andoh, Hiroko Kajita, Yosaburo Oikawa, Minoru Nakao, Makoto Ohnishi, Masahisa Watarai, Hiroshi Shimoda, Ken Maeda, Ai Takano
Borrelia miyamotoi, recently recognized as a human pathogenic spirochete, was isolated from Ixodes persulcatus and I. ovatus in northern Mongolia and Honshu Island, a major island in Japan. Although no human B. miyamotoi infections have been reported in Mongolia, the prevalence of B. miyamotoi in ticks from Mongolia is higher than that in ticks from Hokkaido, Japan, where human cases have been reported. Moreover, the multi-locus sequence analysis of cultured isolates revealed that B. miyamotoi isolates in Mongolia belong to the Siberian type, a sequence type that was originally reported from isolates from I...
July 14, 2017: Ticks and Tick-borne Diseases
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