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Ticks and Tick-borne Diseases

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https://www.readbyqxmd.com/read/28196774/is-the-free-ranging-jaguar-panthera-onca-a-reservoir-for-cytauxzoon-felis-in-brazil
#1
Mariana Malzoni Furtado, Sueli Akemi Taniwaki, Betina Metzger, Karina Dos Santos Paduan, Helena Lucia O'Dwyer, Anah Tereza de Almeida Jácomo, Grasiela E O Porfírio, Leandro Silveira, Rahel Sollmann, Natália Mundim Tôrres, José Soares Ferreira Neto
This study investigated the occurrence of Cytauxzoon felis and Babesia spp. in free-ranging jaguars (Panthera onca), domestic dogs (Canis lupus familiaris) and domestic cats (Felis catus) from the Cerrado, Amazon and Pantanal biomes of Brazil. Blood samples were collected from 30 jaguars, 129 dogs and 22 cats for detection of the 18S rRNA genes of piroplasmids. All of the jaguars from the Pantanal (n=22) and Cerrado (n=4) and three of four jaguars from the Amazon were positive for C. felis, but no dogs or cats were positive for the agent...
February 6, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28196664/new-records-of-ornithodoros-puertoricensis-fox-1947-ixodida-argasidae-parasitizing-humans-in-rural-and-urban-dwellings-panama
#2
Sergio E Bermúdez, Eduardo Castillo, Tyler D Pohlenz, Alexander Kneubehl, Aparna Krishnavajhala, Lillian Domínguez, Antonio Suárez, Job E López
The presence of ticks inside human constructions was evaluated in two localities from Colon province (Charco La Piedra and Espinar) and one from Panama province (Ancon, City of Panama). In two of houses, eight people from Charco La Piedra and one from Ancón reported "insect bites," which produced blisters for several weeks. The investigation resulted in the collection of argasid ticks, which were identified by morphology and sequencing the 16s ribosomal RNA gene, and later evaluated for the presence of relapsing fever Borrelia DNA...
February 5, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28214195/first-clinical-case-of-canine-granulocytic-anaplasmosis-in-korea-and-genotypic-analyses-of-anaplasma-phagocytophilum
#3
Seung-Hun Lee, Nara Kim, Dongmi Kwak
A 2-year-old male Jindo dog was presented to a local veterinary clinic for anorexia, lethargy, edema around neck, and vomiting. Based on the clinical history, physical examination, hematology, blood chemistry, serology, and PCR, the dog was diagnosed with canine granulocytic anaplasmosis (CGA). PCR and phylogenetic analyses targeting the 16S rRNA, groEL, and msp2 genes of Anaplasma phagocytophilum revealed that the A. phagocytophilum identified in this study subgrouped into alanine and USA groups according to the groEL and msp2 gene sequences, respectively...
February 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28188085/species-of-ticks-and-carried-pathogens-in-owned-dogs-in-spain-results-of-a-one-year-national-survey
#4
Agustín Estrada-Peña, Xavier Roura, Angel Sainz, Guadalupe Miró, Laia Solano-Gallego
This study presents the results of a national survey in Spain on the distribution of ticks on owned dogs, their phenology and the associated pathogens over one year. In the study, 1628 adult ticks were collected on 660 dogs presented to 26 veterinary practices, of which 507 dogs (76.8%) carried at least one adult tick. The primary species of ticks were Rhipicephalus sanguineus s.l. (53%), Dermacentor reticulatus (9%), Ixodes ricinus (9%), and I. hexagonus (4%). Parasitism by two species of these ticks was rare...
February 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28174118/molecular-and-structural-characterization-of-novel-cystatins-from-the-taiga-tick-ixodes-persulcatus
#5
Carolina K Rangel, Luís F Parizi, Gabriela A Sabadin, Evenilton P Costa, Nelilma C Romeiro, Masayoshi Isezaki, Naftaly W Githaka, Adriana Seixas, Carlos Logullo, Satoru Konnai, Kazuhiko Ohashi, Itabajara da Silva Vaz
Cystatins are cysteine peptidase inhibitors that in ticks mediate processes such as blood feeding and digestion. The ixodid tick Ixodes persulcatus is endemic to the Eurasia, where it is the principal vector of Lyme borreliosis. To date, no I. persulcatus cystatin has been characterized. In the present work, we describe three novel cystatins from I. persulcatus, named JpIpcys2a, JpIpcys2b and JpIpcys2c. In addition, the potential of tick cystatins as cross-protective antigens was evaluated by vaccination of hamsters using BrBmcys2c, a cystatin from Rhipicephalus microplus, against I...
January 31, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28169171/seasonal-infestation-of-birds-with-immature-stages-of-ixodes-ricinus-and-ixodes-arboricola
#6
Elena Kocianová, Veronika Rusňáková Tarageľová, Danka Haruštiaková, Eva Špitalská
This study assessed the parasitization of cavity-nesting birds and ground-nesting/foraging birds with larvae and nymphs of two Ixodes species, Ixodes ricinus and Ixodes arboricola. Totals of 679 (52.3%) I. ricinus and 619 (47.7%) I. arboricola ticks were collected from 15 species of passerine birds which were caught during the nesting and non-nesting periods of 2003-2006, in the south-eastern part of the Czech Republic, the Drahanská Vrchovina Uplands. In the non-nesting period from October to March, 6.8% (101/1492) of birds were infested with ticks, mainly with I...
January 29, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28143697/first-detection-of-tick-borne-pathogens-of-dogs-from-malta
#7
Elaine Licari, Nóra Takács, Norbert Solymosi, Róbert Farkas
The knowledge about the vector-borne infections in domestic dogs has been increasing worldwide. However no studies have been done on hard tick infestation and tick-borne pathogens (TBPs) of dogs present in Malta. Therefore, a total of 99 dogs was selected and inspected between March and July 2013 in 18 urban and 17 rural areas on the islands of Malta and Gozo. All ticks were removed from the dogs and identified. Blood samples were taken and tested for protozoa (Babesia spp. and Hepatozoon spp.) and bacteria (Anaplasma spp...
January 18, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28169172/molecular-characterisation-of-anaplasma-species-from-african-buffalo-syncerus-caffer-in-kruger-national-park-south-africa
#8
Danielle Sisson, Jasmin Hufschmid, Anna Jolles, Brianna Beechler, Abdul Jabbar
Bovine anaplasmosis is a tick-borne disease, mainly caused by Anaplasma marginale and A. centrale and is distributed in tropical and sub-tropical areas. This study aimed to characterise A. marginale and A. centrale from African buffaloes in Kruger National Park (KNP), South Africa, using the DNA sequences of the genes coding for major surface protein (msp1β) and heat shock protein (groEL), respectively. A total of 747 blood samples were collected from February 2014 to August 2016 from African buffaloes kept in KNP, and DNAs were tested using a molecular-phylogenetic approach...
January 17, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28131594/detection-of-borrelia-miyamotoi-and-other-tick-borne-pathogens-in-human-clinical-specimens-and-ixodes-scapularis-ticks-in-new-york-state-2012-2015
#9
Danielle Wroblewski, Linda Gebhardt, Melissa A Prusinski, Lisa J Meehan, Tanya A Halse, Kimberlee A Musser
Borrelia miyamotoi (Bm) is a recently emerging bacterial agent transmitted by several species of ixodid ticks. Diagnosis of Bm infection can be challenging, as the organism is not easily cultivable. We have developed and validated a multiplex real-time PCR to simultaneously identify Bm infection and the agents causing human granulocytic anaplasmosis and human monocytic ehrlichiosis, Anaplasma phagocytophilum and Ehrlichia chaffeensis, respectively. The assay is 100% specific; highly sensitive, detecting 11 gene copies of Bm DNA in both whole blood and cerebral spinal fluid; and provides rapid results in less than two hours...
January 16, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28109769/molecular-typing-and-diagnosis-of-anaplasma-spp-closely-related-to-anaplasma-phagocytophilum-in-ruminants-from-tunisia
#10
Mourad Ben Said, Hanène Belkahia, Narjesse El Mabrouk, Mariem Saidani, Mariem Ben Hassen, Alberto Alberti, Rosanna Zobba, Skander Bouattour, Ali Bouattour, Lilia Messadi
Accurate diagnosis of animal and zoonotic diseases, such as granulocytic anaplasmosis, is crucial to estimate risk during control programs. In this study, 16S rRNA nested PCR and RFLP assay were combined to investigate the presence of Anaplasma phagocytophilum and genetically related strains (namely A. phagocytophilum-like 1 and 2) in 936 Tunisian ruminants. By using this method, A. phagocytophilum was not detected in any of the tested animals, while A. phagocytophilum-like 1 and A. phagocytophilum-like 2 were detected at variable prevalence rates in sheep, goats and cattle at coinfection rates respectively of 3...
January 16, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28119040/ixodes-ricinus-immunogenic-saliva-protein-homologue-to-amblyomma-americanum-av422-determining-its-potential-for-use-in-tick-bite-confirmation
#11
Darko Mihaljica, Dragana Marković, Željko Radulović, Albert Mulenga, Sanja Ćakić, Ratko Sukara, Jelena Samardžić, Snežana Tomanović
Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I...
January 10, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28117273/novel-sub-lineages-recombinants-and-reassortants-of-severe-fever-with-thrombocytopenia-syndrome-virus
#12
Qiang Lv, Hong Zhang, Lei Tian, Ruiling Zhang, Zhenjie Zhang, Juan Li, Yigang Tong, Hang Fan, Michael J Carr, Weifeng Shi
Severe fever with thrombocytopenia syndrome virus (SFTSV) has been continuously circulating in Eastern Asia in recent years, without the availability of effective vaccines or antiviral drugs, posing a serious threat to public health, particularly in the central-eastern provinces of China. In this study, we isolated and sequenced four new SFTSV strains from Shandong Province identified in 2015. Phylogenetic analysis, with all publicly available L, M and S gene segments, revealed that the four newly described SFTSV strains belonged to genotype C3...
January 3, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28063831/life-cycle-and-parasitic-competence-of-dermacentor-nitens-neumann-1897-acari-ixodidae-on-different-animal-species
#13
Vinicius da Silva Rodrigues, Marcos Valério Garcia, Breno Cayeiro Cruz, Willian Giquelin Maciel, Namor Pinheiro Zimmermann, Wilson Werner Koller, Jacqueline Cavalcante Barros, Renato Andreotti
The objective of this study was to evaluate the life cycle and parasitic competence of Dermacentor nitens (Neumann, 1897) on different animal species. Experimental infestations were induced in five specimens each of seven species of possible hosts: rabbits, horses, sheep, cows, guinea pigs, birds and dogs. Rabbits were infested in the ear using artificial feeding chambers, and the horses, sheep, cows and dogs were infested in the ear without feeding chambers. For the infestation of guinea pigs, artificial feeding chambers were fixed on the back...
December 31, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28065617/borrelia-miyamotoi-in-vectors-and-hosts-in-the-netherlands
#14
Alex Wagemakers, Seta Jahfari, Bob de Wever, Lodewijk Spanjaard, Markus V Starink, Henry J C de Vries, Hein Sprong, Joppe W Hovius
Ixodes ticks transmit Borrelia burgdorferi sensu lato (s.l.), the causative agent of Lyme borreliosis (LB). These tick species also transmit Borrelia miyamotoi, which was recently found to cause infections in humans. We were interested in the prevalence of B. miyamotoi infection in ticks and natural hosts in The Netherlands, and to what extent ticks are co-infected with B. burgdorferi. In addition, erythema migrans has been sporadically described in B. miyamotoi-infected patients, but these skin lesions might as well represent co-infections with B...
December 30, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28063832/the-risk-of-exposure-to-rickettsial-infections-and-human-granulocytic-anaplasmosis-associated-with-ixodes-ricinus-tick-bites-in-humans-in-romania-a-multiannual-study
#15
Ioana Adriana Matei, Zsuzsa Kalmár, Mihaela Lupşe, Gianluca D'Amico, Angela Monica Ionică, Mirabela Oana Dumitrache, Călin Mircea Gherman, Andrei Daniel Mihalca
Anaplasma phagocytophilum and spotted fever group Rickettsia are obligate intracellular Gram-negative tick-borne bacteria, among which several may cause clinical infections in humans. Several Rickettsia spp. and A. phagocytophilum are transmitted in Europe by Ixodes ricinus, the most common tick species feeding on humans in this area. The aim of this study was to evaluate the annual prevalence of Rickettsia spp. and A. phagocytophilum in I. ricinus collected from humans during three consecutive years. The mean prevalences of the infection with the investigated pathogens in I...
December 29, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28089651/canine-tick-borne-pathogens-in-cyprus-and-a-unique-canine-case-of-multiple-co-infections
#16
Charalampos Attipa, Chelsea A E Hicks, Emily N Barker, Vasiliki Christodoulou, Kyriaki Neofytou, Mathios E Mylonakis, Victoria I Siarkou, Elpida I Vingopoulou, Francesca Soutter, Dimosthenis Chochlakis, Anna Psaroulaki, Kostas Papasouliotis, Séverine Tasker
Canine tick-borne pathogens such as Ehrlichia canis and Hepatozoon canis are widespread in the Mediterranean basin but have never been reported or investigated in Cyprus. We describe herein the presence of canine tick-borne pathogens in three dogs with clinical signs compatible with vector-borne diseases from Paphos area of Cyprus. Molecular and phylogenetic analysis revealed the presence of E. canis, Anaplasma platys, H. canis, Babesia vogeli and Mycoplasma haemocanis in Cyprus. One dog co-infected with E...
December 26, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28089124/local-scale-spatio-temporal-distribution-of-questing-ixodes-ricinus-l-acari-ixodidae-a-case-study-from-a-riparian-urban-forest-in-wroc%C3%A5-aw-sw-poland
#17
Dorota Kiewra, Ewa Stefańska-Krzaczek, Mariusz Szymanowski, Anna Szczepańska
This paper presents the distribution of questing Ixodes ricinus ticks in suburban forest intensively visited by people. The local-scale observations conducted during a 4-year study at 99 plots (of 100m(2) each) located throughout the entire area of a riparian urban forest, showed a high variation in the density of ticks from year to year. Although I. ricinus is generally permanent in the study area, spatial distribution of sample plots harbouring I. ricinus is variable, i.e. mainly random for adults and larvae, and random or clustered for nymphs...
December 23, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28089123/ticks-and-borrelia-in-urban-and-peri-urban-green-space-habitats-in-a-city-in-southern-england
#18
Kayleigh M Hansford, Manoj Fonville, Emma L Gillingham, Elena Claudia Coipan, Maaike E Pietzsch, Aleksandra I Krawczyk, Alexander G C Vaux, Benjamin Cull, Hein Sprong, Jolyon M Medlock
Ticks are becoming increasingly recognised as important vectors of pathogens in urban and peri-urban areas, including green space used for recreational activities. In the UK, the risk posed by ticks in such areas is largely unknown. In order to begin to assess the risk of ticks in urban/peri-urban areas in southern England, questing ticks were collected from five different habitat types (grassland, hedge, park, woodland and woodland edge) in a city during the spring, summer and autumn of 2013/2014 and screened for Borrelia burgdorferi sensu lato...
December 21, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28017622/first-report-of-exotic-ticks-amblyomma-rotundatum-parasitizing-invasive-cane-toads-rhinella-marina-on-the-island-of-hawai-i
#19
Crystal Kelehear, Cameron M Hudson, James W Mertins, Richard Shine
Our surveys of 1401 invasive cane toads (Rhinella marina) from the Hawaiian islands of Hawai'i, O'ahu, and Maui revealed the presence of an exotic tick, Amblyomma rotundatum. Immature and adult female ticks infested three wild adult toads at a single site in the vicinity of a zoo south of Hilo, Island of Hawai'i, Hawai'i, USA. We found no tick-infested toads on O'ahu or Maui. This tick infests cane toads in their native Neotropical range, but it was excluded from Hawai'i when the original founder toads were introduced over 80 years ago...
December 19, 2016: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28110915/detection-of-rickettsia-hoogstraalii-rickettsia-helvetica-rickettsia-massiliae-rickettsia-slovaca-and-rickettsia-aeschlimannii-in-ticks-from-sardinia-italy
#20
Valentina Chisu, Hamza Leulmi, Giovanna Masala, Mariano Piredda, Cipriano Foxi, Philippe Parola
Tick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. The presence of Rickettsia spp. was evaluated by standard PCR and sequencing in 123 ticks collected from several mammals and vegetation in Sardinia, Italy. This study provides the first evidence of the presence of Rickettsia hoogstralii in Haemaphysalis punctata and Haemaphysalis sulcata ticks from mouflon and Rickettsia helvetica in Ixodes festai ticks from hedgehog. In addition, Rickettsia massiliae, Rickettsia slovaca and Rickettsia aeschlimannii were detected in Rhipicephalus sanguineus, Dermacentor marginatus and Hyalomma marginatum marginatum ticks from foxes, swine, wild boars, and mouflon...
December 15, 2016: Ticks and Tick-borne Diseases
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