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Biofabrication

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https://www.readbyqxmd.com/read/28726681/a-novel-cylindrical-microwell-featuring-inverted-pyramidal-opening-for-efficient-cell-spheroid-formation-without-cell-loss
#1
Jae Min Cha, HyungDal Park, Eun Kyoung Shin, Ji Hee Sung, Ockchul Kim, Woohyun Jung, Oh Young Bang, Jinseok Kim
Spheroid cultures have been often used to simulate and understand in situ biological occurrences with potential to be further applied to therapeutic approaches, such as cell transplantation. However, traditional lab-scale techniques hardly reached the needed large scale production of cell spheroids, thus limiting their versatility in many biomedical fields. Microscale technologies have rapidly improved in the last decade, and contributed to the large scale production of cell spheroids with high controllability and reproducibility...
July 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28726680/towards-4d-printed-scaffolds-for-tissue-engineering-exploiting-3d-shape-memory-polymers-to-deliver-time-controlled-stimulus-on-cultured-cells
#2
Wim Hendrikson, Jeroen Rouwkema, Federico Clementi, Clemens A van Blitterswijk, Silvia Fare, Lorenzo Moroni
Tissue engineering needs innovative solutions to better fit the requirements of a minimally-invasive approach, providing at the same time instructive cues to cells. The use of shape memory polyurethane has been investigated by producing 4D scaffolds via 3D printing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized by dynamic-mechanical analysis (DMA). The thermo-mechanical analysis showed a Tg at about 32 °C (Tg = Ttrans), indicating no influence of the fabrication process on the transition temperature...
July 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28707627/mussel-inspired-nano-building-block-assemblies-for-mimicking-extracellular-matrix-microenvironments-with-multiple-functions
#3
Zhenming Wang, Zhanrong Jia, Yanan Jiang, Pengfei Li, Lu Han, Xiong Lu, Fuzeng Ren, Ke-Feng Wang, Huipin Yuan
The assembly of nano-building blocks is an effective way to produce artificial extracellular matrix microenvironments with hierarchical micro/nano structures. However, it is hard to assemble different types of nano-building blocks, to form composite coatings with multiple functions, by traditional layer-by-layer (LbL) self-assembly methods. Inspired by the mussel adhesion mechanism, we developed polydopamine (PDA)-decorated bovine serum albumin nanoparticles (BSA-NPs) and nano-hydroxyapatite (nano-HA), and assembled them to form bioactive coatings with micro/nano structures encapsulating bone morphogenetic protein-2 (BMP-2)...
July 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28707625/bioprinting-of-functional-vascularized-mouse-thyroid-gland-construct
#4
Elena A Bulanova, Elizaveta V Koudan, Jonathan Degosserie, Charlotte Heymans, Frederico DAS Pereira, Vladislav A Parfenov, Yi Sun, Qi Wang, Suraya A Akhmedova, Irina K Sviridova, Natalia S Sergeeva, Georgy A Frank, Yusef D Khesuani, Christophe E Pierreux, Vladimir A Mironov
Bioprinting can be defined as additive biofabrication of 3D tissues and organ constructs using tissue spheroids, capable of self-assembly, as building blocks. Thyroid gland, a relatively simple endocrine organ, is suitable for testing the proposed bioprinting technology. Here we report the bioprinting of functional vascularized mouse thyroid gland construct from embryonic tissue spheroids as a proof of concept. Based on the self-assembly principle, we generated thyroid tissue starting from thyroid spheroids (TS) and allantoic spheroids (AS), as a source of thyrocytes and endothelial cells (EC), respectively...
July 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28707624/three-dimensional-3d-culture-in-sarcoma-research-and-the-clinical-significance
#5
Songtao Gao, Jacson Shen, Francis Hornicek, Zhenfeng Duan
Sarcomas are rare malignant tumors that arise from transformed cells of mesenchymal origin. Despite the progress in diagnosis and treatment, sarcomas have a high mortality rate due to local recurrence, metastasis, and the development of drug resistance to chemotherapy. New models for sarcoma research are required to further understand the disease and to develop new therapies. In vitro sarcoma modeling is challenging because of significant genetic heterogeneities, diverse pathological, and overlapping clinical characteristics...
July 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28691696/decellularized-extracellular-matrix-a-step-towards-the-next-generation-source-for-bioink-manufacturing
#6
Byoung Soo Kim, Hyeonji Kim, Ge Gao, Jinah Jang, Dong-Woo Cho
In tissue engineering, the need for hierarchical assembly of three-dimensional (3D) tissues becomes increasingly important, considering that a new technology is essential for advanced tissue fabrication. 3D cell printing has emerged as a powerful technology to recapitulate the microenvironment of native tissue, allowing for the precise deposition of multiple cells onto the pre-defined position. Parallel to these technological advances, seeking for an appropriate bioink to provide a suitable microenvironment supporting cellular activities has been into the spotlight...
July 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28691691/development-of-a-clay-based-bioink-for-3d-cell-printing-for-skeletal-application
#7
Tilman Ahlfeld, Gianluca Cidonio, David Kilian, Sarah Duin, Ashwini Rahul Akkineni, Jon I Dawson, Shoufeng Yang, Anja Lode, Richard Oreffo, Michael Gelinsky
Three-dimensional printing of cell-laden hydrogels has evolved as a promising approach on the route to patient-specific or complex tissue engineered constructs. However, it is still challenging to print structures with both, high shape fidelity and cell vitality. Herein, we used a synthetic nanosilicate clay, called Laponite, to build up scaffolds utilizing the extrusion-based method 3D plotting. By blending with alginate and methylcellulose, a bioink was developed which allowed easy extrusion, achieving scaffolds with high printing fidelity...
July 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28671108/cell-instructive-high-resolution-micropatterned-polylactic-acid-surfaces
#8
David Baiao Barata, Paulo Dias, Paul Wieringa, Clemens A van Blitterswijk, Pamela Habibovic
Micro- and nanoscale topographical structuring of biomaterial surfaces has been a valuable tool for influencing cell behavior, including cell attachment, proliferation and differentiation. However, most fabrication techniques for surface patterning of implantable biomaterials suffer from a limited resolution, not allowing controlled generation of sub-cellular three-dimensional features. Here, a direct laser lithography technique based on two-photon absorption was used to construct several patterns varying in size between 500 nm and 15 µm...
July 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28639943/bioprintable-form-of-chitosan-hydrogel-for-bone-tissue-engineering
#9
T Tolga Demirtas, Gülseren Irmak, Menemse Gumusderelioglu
Bioprinting can be defined as the 3D patterning of living cells and other biologics by using a computer-aided layer-by-layer deposition approach. Presence of cells within the ink to usage of a "bio-ink" presents the potential to print of living tissues and organ analogs that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration. In this study, it was shown for the first time that chitosan solution and its composite with nanostructured bone-like hydroxyapatite (HA) can be mixed with cells and printed successfully...
June 22, 2017: Biofabrication
https://www.readbyqxmd.com/read/28604361/synthetic-scaffolds-with-full-pore-interconnectivity-for-bone-regeneration-prepared-by-supercritical-foaming-using-advanced-biofunctional-plasticizers
#10
Aurelio Salerno, Sara Diéguez, Luis Diaz-Gomez, Jose Luis Gomez-Amoza, Beatriz Magariños, Angel Concheiro, Concepcion Domingo, Carmen Alvarez-Lorenzo, Carlos A García-González
Supercritical foaming allows for the solvent-free processing of synthetic scaffolds for bone regeneration. However, the control on the pore interconnectivity and throat pore size with this technique still needs to be improved. The use of plasticizers may help to overcome these limitations. Eugenol, a GRAS natural compound extracted from plants, is proposed in this work as an advanced plasticizer with bioactive properties. Eugenol-containing poly(ɛ-caprolactone) (PCL) scaffolds were obtained by supercritical foaming (20...
June 12, 2017: Biofabrication
https://www.readbyqxmd.com/read/28589919/alternately-plasma-roughened-nanosurface-of-a-hybrid-scaffold-for-aligning-myoblasts
#11
Gi Hoon Yang, Hojun Jeon, GeunHyung Kim
For successful skeletal muscle tissue regeneration, inducing alignment and fusion of myoblasts into multinucleated myotubes is critical. Many studies are ongoing to induce myoblast alignment using various micro/nanopatternings on scaffold surfaces, mechanically stretching scaffolds, or aligned micro/nanofibers. In this study, we have developed a simple method to induce myoblast alignment using a modified plasma treatment on a hybrid PCL scaffold consisting of melt-printed perpendicular PCL struts and an electrospun PCL fibrous mat...
June 7, 2017: Biofabrication
https://www.readbyqxmd.com/read/28569243/cell-sheet-based-bioink-for-3d-bioprinting-applications
#12
Ezgi Bakirci, Burak Toprakhisar, Mehmet Zeybek, Gozde Ozaydin Ince, Bahattin Koc
In this research, a novel development of bioink from cell sheets is presented for scaffold free bioprinting applications. Poly(N-isopropylacrylamide) (PNIPAAm) coated surfaces were first prepared by using initiated Chemical Vapor Deposition (iCVD) method. Cell-sheets were then grown on these thermoresposnsive pNIPAAm coated surfaces and easily detached without disturbing delicate cell-extracellular matrix (ECM) and cell-cell interactions. The detached cells sheets were used to prepare cell sheet based bioink and bioprinted to form various shapes...
June 1, 2017: Biofabrication
https://www.readbyqxmd.com/read/28504242/simultaneous-microfluidic-spinning-of-multiple-strands-of-submicron-fiber-for-the-production-of-free-standing-porous-membranes-for-biological-application
#13
DoYeun Park, JiSoo Park, Heeyeong Jang, Jie Cheng, Soo-Hyun Kim, Sang-Hoon Lee
Microfibers produced using electrospinning and microfluidics-based technologies have been developed as a powerful tool in tissue engineering applications such as drug delivery and scaffolds. The applications of these fibers, however, have been limited because of the hazardous solvents used to make them, difficulties in controlling the pore sizes of their membrane forms, and downscaling the size of the fiber. Nevertheless, extending the use of these fibers such as the production of a free-standing porous membrane appropriate for cell-based research is highly needed for tissue engineering, organ-on-a-chip, and drug delivery research and applications...
May 15, 2017: Biofabrication
https://www.readbyqxmd.com/read/28488594/large-scale-production-and-controlled-deposition-of-single-huvec-spheroids-for-bioprinting-applications
#14
Ludwig Gutzweiler, Sabrina Kartmann, Kevin Troendle, Leo Benning, Günter Finkenzeller, Roland Zengerle, Peter Koltay, Bjoern Stark, Stefan Zimmermann
We present 1.) a fast and automated method for large scale production of HUVEC spheroids based on the hanging drop method and 2.) a novel method for well-controlled lateral deposition of single spheroids by drop-on-demand printing. Large scale spheroid production is achieved via printing 1536 droplets of HUVEC cell suspension having a volume of 1 µl each within 3 minutes at a pitch of 2.3 mm within an array of 48 x 32 droplets onto a flat substrate. Printing efficiencies between 97.9% and 100% and plating efficiencies between 87...
May 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28488588/leaf-inspired-microcontact-printing-vascular-patterns
#15
Lian Wong, John Pegan, Basia Gabela-Zuniga, Michelle Khine, Kara E McCloskey
The vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft. A number of strategies are currently being investigated including very promising microfluidics systems. Here, we explored the potential for generating a vasculature-patterned endothelial cells (EC) that could be integrated into distinct layers between sheets of primary cells. Bioinspired from the leaf veins, we generated a reverse mold with a fractal vascular-branching pattern that models the unique spatial arrangement over multiple length scales that precisely mimic branching vasculature...
May 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28485302/cell-patterning-via-laser-micro-nano-structured-silicon-surfaces
#16
Christina Yiannakou, Chara Simitzi, Aleka Manousaki, Costas Fotakis, Anthi Ranella, Emmanuel Stratakis
The surface topography of biomaterials can have an important impact on the cellular adhesion, growth and proliferation. Apart from the overall roughness, the detailed morphological features at all length scales significantly affect the cell-biomaterial interactions in a plethora of applications including structural implants, tissue engineering scaffolds and biosensors. In this study, we present a simple, one-step direct laser patterning technique to fabricate nanoripples and dual-rough hierarchical micro/nano structures to control SW10 cell attachment and migration...
May 9, 2017: Biofabrication
https://www.readbyqxmd.com/read/28485301/developing-organ-on-a-chip-concepts-using-bio-mechatronic-design-methodology
#17
Jonas Christoffersson, Danny van van Noort, C-F Mandenius
Mechatronic design is an engineering methodology for conceiving, configuring, and optimising the design of a technical device or product to the needs and requirements of the final user. In this article, we show how the basic principles of this methodology can be exploited for in vitro cell cultures - often referred to as organ-on-a-chip devices. Due to the key role of the biological cells, we have introduced the term bio-mechatronic design, to highlight the complexity of designing a system that should integrate biology, mechanics and electronics in the same device structure...
May 9, 2017: Biofabrication
https://www.readbyqxmd.com/read/28471354/introducing-biomimetic-shear-and-ion-gradients-to-microfluidic-spinning-improves-silk-fiber-strength
#18
David Li, Matthew Jacobsen, Nae Rim, Daniel Backman, David Kaplan, Joyce Y Wong
Silkworm silk is an attractive biopolymer for biomedical applications due to its high mechanical strength and biocompatibility; as a result, there is increasing interest in scalable devices to spin silk and recombinant silk so as to improve and customize their properties for diverse biomedical purposes.[1] While artificial spinning of regenerated silk fibroins adds tunability to properties such as degradation rate and surface functionalization, the resulting fibers do not yet approach the mechanical strength of native silkworm silk...
May 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28467316/the-fabrication-of-double-layered-chitosan-gelatin-genipin-nanosphere-coating-for-sequential-and-controlled-release-of-therapeutic-proteins
#19
Ruiying Chen, Xinjie Cai, Kena Ma, Yi Zhou, Yining Wang, Tao Jiang
Bone regeneration is a complicated process and includes a number of distinct and sequential stages of coordinated cellular actions under the regulation of multiple growth factors. Therefore, bone grafting materials in which growth factors can be incorporated and released in a programmed order in line with the bone tissue healing process may lead to desirable clinical outcomes. In the present study, a double-layered chitosan/gelatin/genipin (d-CSG/G) nanosphere coating is developed by using layer-by-layer electrophoretic deposition (EPD) and genipin crosslinking...
May 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28462910/optimum-silicon-doping-of-carbon-coatings-by-pulsed-laser-technique-for-superior-functional-biomedical-surfaces-fabrication
#20
Ion N Mihailescu, Dorota Bociaga, Gianina Popescu-Pelin, George Stan, Liviu Duta, Gabriel Socol, Carmen Chifiriuc, Coralia Bleotu, Veronica Lazar, Marius-Adrian Husanu, Irina Zgura, Florin Miculescu, Irina Negut, Claudiu Hapenciuc
We report on the fabrication of Silicon (Si) doped Carbon (C) [C:Si] structures by combinatorial pulsed laser deposition to identify the best design for a novel generation of multi-functional coated implants. The synthesized films were characterized from the morphological, structural, compositional, mechanical and microbiological points of view. Scanning Electron Microscopy investigation highlighted the presence, on top of the deposited layers, of spheroid particulates with sizes in the micron range. No micro-cracks or delaminations were observed...
May 2, 2017: Biofabrication
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