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Biofabrication

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https://www.readbyqxmd.com/read/28639943/bioprintable-form-of-chitosan-hydrogel-for-bone-tissue-engineering
#1
T Tolga Demirtas, Gülseren Irmak, Menemse Gumusderelioglu
Bioprinting can be defined as the 3D patterning of living cells and other biologics by using a computer-aided layer-by-layer deposition approach. Presence of cells within the ink to usage of a "bio-ink" presents the potential to print of living tissues and organ analogs that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration. In this study, it was shown for the first time that chitosan solution and its composite with nanostructured bone-like hydroxyapatite (HA) can be mixed with cells and printed successfully...
June 22, 2017: Biofabrication
https://www.readbyqxmd.com/read/28604361/synthetic-scaffolds-with-full-pore-interconnectivity-for-bone-regeneration-prepared-by-supercritical-foaming-using-advanced-biofunctional-plasticizers
#2
Aurelio Salerno, Sara Diéguez, Luis Diaz-Gomez, Jose Luis Gomez-Amoza, Beatriz Magariños, Angel Concheiro, Concepcion Domingo, Carmen Alvarez-Lorenzo, Carlos A García-González
Supercritical foaming allows for the solvent-free processing of synthetic scaffolds for bone regeneration. However, the control on the pore interconnectivity and throat pore size with this technique still needs to be improved. The use of plasticizers may help to overcome these limitations. Eugenol, a GRAS natural compound extracted from plants, is proposed in this work as an advanced plasticizer with bioactive properties. Eugenol-containing poly(ɛ-caprolactone) (PCL) scaffolds were obtained by supercritical foaming (20...
June 12, 2017: Biofabrication
https://www.readbyqxmd.com/read/28589919/alternately-plasma-roughened-nanosurface-of-a-hybrid-scaffold-for-aligning-myoblasts
#3
Gi Hoon Yang, Hojun Jeon, GeunHyung Kim
For successful skeletal muscle tissue regeneration, inducing alignment and fusion of myoblasts into multinucleated myotubes is critical. Many studies are ongoing to induce myoblast alignment using various micro/nanopatternings on scaffold surfaces, mechanically stretching scaffolds, or aligned micro/nanofibers. In this study, we have developed a simple method to induce myoblast alignment using a modified plasma treatment on a hybrid PCL scaffold consisting of melt-printed perpendicular PCL struts and an electrospun PCL fibrous mat...
June 7, 2017: Biofabrication
https://www.readbyqxmd.com/read/28569243/cell-sheet-based-bioink-for-3d-bioprinting-applications
#4
Ezgi Bakirci, Burak Toprakhisar, Mehmet Zeybek, Gozde Ozaydin Ince, Bahattin Koc
In this research, a novel development of bioink from cell sheets is presented for scaffold free bioprinting applications. Poly(N-isopropylacrylamide) (PNIPAAm) coated surfaces were first prepared by using initiated Chemical Vapor Deposition (iCVD) method. Cell-sheets were then grown on these thermoresposnsive pNIPAAm coated surfaces and easily detached without disturbing delicate cell-extracellular matrix (ECM) and cell-cell interactions. The detached cells sheets were used to prepare cell sheet based bioink and bioprinted to form various shapes...
June 1, 2017: Biofabrication
https://www.readbyqxmd.com/read/28504242/simultaneous-microfluidic-spinning-of-multiple-strands-of-submicron-fiber-for-the-production-of-free-standing-porous-membranes-for-biological-application
#5
DoYeun Park, JiSoo Park, Heeyeong Jang, Jie Cheng, Soo-Hyun Kim, Sang-Hoon Lee
Microfibers produced using electrospinning and microfluidics-based technologies have been developed as a powerful tool in tissue engineering applications such as drug delivery and scaffolds. The applications of these fibers, however, have been limited because of the hazardous solvents used to make them, difficulties in controlling the pore sizes of their membrane forms, and downscaling the size of the fiber. Nevertheless, extending the use of these fibers such as the production of a free-standing porous membrane appropriate for cell-based research is highly needed for tissue engineering, organ-on-a-chip, and drug delivery research and applications...
May 15, 2017: Biofabrication
https://www.readbyqxmd.com/read/28488594/large-scale-production-and-controlled-deposition-of-single-huvec-spheroids-for-bioprinting-applications
#6
Ludwig Gutzweiler, Sabrina Kartmann, Kevin Troendle, Leo Benning, Günter Finkenzeller, Roland Zengerle, Peter Koltay, Bjoern Stark, Stefan Zimmermann
We present 1.) a fast and automated method for large scale production of HUVEC spheroids based on the hanging drop method and 2.) a novel method for well-controlled lateral deposition of single spheroids by drop-on-demand printing. Large scale spheroid production is achieved via printing 1536 droplets of HUVEC cell suspension having a volume of 1 µl each within 3 minutes at a pitch of 2.3 mm within an array of 48 x 32 droplets onto a flat substrate. Printing efficiencies between 97.9% and 100% and plating efficiencies between 87...
May 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28488588/leaf-inspired-microcontact-printing-vascular-patterns
#7
Lian Wong, John Pegan, Basia Gabela-Zuniga, Michelle Khine, Kara E McCloskey
The vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft. A number of strategies are currently being investigated including very promising microfluidics systems. Here, we explored the potential for generating a vasculature-patterned endothelial cells (EC) that could be integrated into distinct layers between sheets of primary cells. Bioinspired from the leaf veins, we generated a reverse mold with a fractal vascular-branching pattern that models the unique spatial arrangement over multiple length scales that precisely mimic branching vasculature...
May 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28485302/cell-patterning-via-laser-micro-nano-structured-silicon-surfaces
#8
Christina Yiannakou, Chara Simitzi, Aleka Manousaki, Costas Fotakis, Anthi Ranella, Emmanuel Stratakis
The surface topography of biomaterials can have an important impact on the cellular adhesion, growth and proliferation. Apart from the overall roughness, the detailed morphological features at all length scales significantly affect the cell-biomaterial interactions in a plethora of applications including structural implants, tissue engineering scaffolds and biosensors. In this study, we present a simple, one-step direct laser patterning technique to fabricate nanoripples and dual-rough hierarchical micro/nano structures to control SW10 cell attachment and migration...
May 9, 2017: Biofabrication
https://www.readbyqxmd.com/read/28485301/developing-organ-on-a-chip-concepts-using-bio-mechatronic-design-methodology
#9
Jonas Christoffersson, Danny van van Noort, C-F Mandenius
Mechatronic design is an engineering methodology for conceiving, configuring, and optimising the design of a technical device or product to the needs and requirements of the final user. In this article, we show how the basic principles of this methodology can be exploited for in vitro cell cultures - often referred to as organ-on-a-chip devices. Due to the key role of the biological cells, we have introduced the term bio-mechatronic design, to highlight the complexity of designing a system that should integrate biology, mechanics and electronics in the same device structure...
May 9, 2017: Biofabrication
https://www.readbyqxmd.com/read/28471354/introducing-biomimetic-shear-and-ion-gradients-to-microfluidic-spinning-improves-silk-fiber-strength
#10
David Li, Matthew Jacobsen, Nae Rim, Daniel Backman, David Kaplan, Joyce Y Wong
Silkworm silk is an attractive biopolymer for biomedical applications due to its high mechanical strength and biocompatibility; as a result, there is increasing interest in scalable devices to spin silk and recombinant silk so as to improve and customize their properties for diverse biomedical purposes.[1] While artificial spinning of regenerated silk fibroins adds tunability to properties such as degradation rate and surface functionalization, the resulting fibers do not yet approach the mechanical strength of native silkworm silk...
May 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28467316/the-fabrication-of-double-layered-chitosan-gelatin-genipin-nanosphere-coating-for-sequential-and-controlled-release-of-therapeutic-proteins
#11
Ruiying Chen, Xinjie Cai, Kena Ma, Yi Zhou, Yining Wang, Tao Jiang
Bone regeneration is a complicated process and includes a number of distinct and sequential stages of coordinated cellular actions under the regulation of multiple growth factors. Therefore, bone grafting materials in which growth factors can be incorporated and released in a programmed order in line with the bone tissue healing process may lead to desirable clinical outcomes. In the present study, a double-layered chitosan/gelatin/genipin (d-CSG/G) nanosphere coating is developed by using layer-by-layer electrophoretic deposition (EPD) and genipin crosslinking...
May 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28462910/optimum-silicon-doping-of-carbon-coatings-by-pulsed-laser-technique-for-superior-functional-biomedical-surfaces-fabrication
#12
Ion N Mihailescu, Dorota Bociaga, Gianina Popescu-Pelin, George Stan, Liviu Duta, Gabriel Socol, Carmen Chifiriuc, Coralia Bleotu, Veronica Lazar, Marius-Adrian Husanu, Irina Zgura, Florin Miculescu, Irina Negut, Claudiu Hapenciuc
We report on the fabrication of Silicon (Si) doped Carbon (C) [C:Si] structures by combinatorial pulsed laser deposition to identify the best design for a novel generation of multi-functional coated implants. The synthesized films were characterized from the morphological, structural, compositional, mechanical and microbiological points of view. Scanning Electron Microscopy investigation highlighted the presence, on top of the deposited layers, of spheroid particulates with sizes in the micron range. No micro-cracks or delaminations were observed...
May 2, 2017: Biofabrication
https://www.readbyqxmd.com/read/28462906/biomimetic-design-and-fabrication-of-multilayered-osteochondral-scaffolds-by-low-temperature-deposition-manufacturing-and-thermal-induced-phase-separation-techniques
#13
Ting Zhang, Hefeng Zhang, Laquan Zhang, Shuaijun Jia, Jian Liu, Zhuo Xiong, Wei Sun
Integrative osteochondral repair is a useful strategy for cartilage-defect repair. To mimic the microenvironment, it is necessary that scaffolds effectively mimic the extracellular matrix of natural cartilage and subchondral bone. In this study, biomimetic osteochondral scaffolds containing an oriented cartilage layer, a compact layer, and a three-dimensional (3D)-printed core-sheath structured-bone layer were developed. The oriented cartilage layer was designed to mimic the structural and material characteristics of native cartilage tissue and was fabricated with cartilage matrix-chitosan materials, using thermal-induced phase-separation technology...
May 2, 2017: Biofabrication
https://www.readbyqxmd.com/read/28382923/a-compliant-and-biomimetic-three-layered-vascular-graft-for-small-blood-vessels
#14
Yi Zhang, Xiangshun Li, Géraldine Guex, Shanshan Liu, Eike Müller, Riccardo Innocenti Malini, Huijing Zhao, Markus Rottmar, Katharina Maniura, Rene Rossi, Fabrizio Spano
Engineering a small diameter vascular graft with mechanical and biological properties comparable to living tissues remains challenging. Often, current devices lead to thrombosis and unsatisfactory long-term patency as a result of poor blood compatibility and a mismatch between the mechanical properties of the living tissue and the implanted biomaterial. Address-ing all these requirements is essential to produce scaffolds able to survive throughout the life of the patient. For this purpose, we fabricated a novel three-layered vascular graft by combining electrospinning and braiding...
April 6, 2017: Biofabrication
https://www.readbyqxmd.com/read/28374682/biofabricated-soft-network-composites-for-cartilage-tissue-engineering
#15
Onur Bas, Elena De-Juan-Pardo, Christoph Meinert, Davide D'Angella, Jeremy Baldwin, Laura Bray, R Wellard, Stefan Kollmannsberger, Ernst Rank, Carsten Werner, Travis Klein, Isabelle Catelas, Dietmar Werner Hutmacher
Articular cartilage from a material science point of view is a soft network composite that plays a critical role in load-bearing joints during dynamic loading. Its composite structure, consisting of a collagen fiber network and a hydrated proteoglycan matrix, gives rise to the complex mechanical properties of the tissue including viscoelasticity and stress relaxation. Melt Electrospinning Writing (MEW) allows the design and fabrication of medical grade polycaprolactone (mPCL) fibrous networks for the reinforcement of soft hydrogel matrices for cartilage tissue engineering...
April 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28631624/printing-induced-cell-injury-evaluation-during-laser-printing-of-3t3-mouse-fibroblasts
#16
Zhengyi Zhang, Wenxuan Chai, Ruitong Xiong, Lei Zhou, Yong Huang
Three-dimensional bioprinting has emerged as a promising solution for the freeform fabrication of living cellular constructs, which can be used for tissue/organ transplantation and tissue models. During bioprinting, some living cells are unavoidably injured and may become necrotic or apoptotic cells. This study aims to investigate the printing-induced cell injury and evaluates injury types of post-printing cells using the annexin V/7-aminoactinomycin D and FAM-DEVD-FMK/propidium iodide assays during laser printing of NIH 3T3 mouse fibroblasts...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28631614/3d-printed-bioceramic-scaffolds-with-antibacterial-and-osteogenic-activity
#17
Yongliang Zhang, Dong Zhai, Mengchi Xu, Qingqiang Yao, Huiying Zhu, Jiang Chang, Chengtie Wu
Bacterial infection poses a significant risk with the wide application of bone graft materials. Designing bone grafts with good antibacterial performance and excellent bone-forming activity is of particular significance for bone tissue engineering. In our study, a 3D printing method was used to prepare β-tricalcium phosphate (β-TCP) bioceramic scaffolds. Silver (Ag) nanoparticles were uniformly dispersed on graphene oxide (GO) to form a homogeneous nanocomposite (named Ag@GO) with different Ag-to-graphene oxide mass ratios, with this being synthesized via the liquid chemical reduction approach...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28631613/repairing-a-bone-defect-with-a-three-dimensional-cellular-construct-composed-of-a-multi-layered-cell-sheet-on-electrospun-mesh
#18
Zhiwei Ren, Shiqing Ma, Le Jin, Zihao Liu, Deping Liu, Xu Zhang, Qing Cai, Xiaoping Yang
In addition to providing maneuverability, electrospun nanofibrous meshes can make excellent supports for constructing flexible cell sheets to regulate cell behavior by nanofiber features. With the target of bone regeneration, herein composite nanofibers with two different fiber arrangements (nestlike, random) were electrospun from a blend solution containing poly(l-lactide) (PLLA) and gelatin (1:1 in weight ratio). Unlike the non-woven morphology in a random nanofibrous mesh, PLLA/gelatin composite nanofibers in the nestlike nanofibrous mesh displayed both non-woven and parallel morphologies...
June 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28617667/a-heuristic-computational-model-of-basic-cellular-processes-and-oxygenation-during-spheroid-dependent-biofabrication
#19
T J Sego, U Kasacheuski, D Hauersperger, A Tovar, N I Moldovan
An emerging approach in biofabrication is the creation of 3D tissue constructs through scaffold-free, cell spheroid-only methods. The basic mechanism in this technology is spheroid fusion, which is driven by the minimization of energy, the same biophysical mechanism that governs spheroid formation. However, other factors such as oxygen and metabolite accessibility within spheroids impact on spheroid properties and their ability to form larger-scale structures. The goal of our work is to develop a simulation platform eventually capable of predicting the conditions that minimize metabolism-related cell loss within spheroids...
June 15, 2017: Biofabrication
https://www.readbyqxmd.com/read/28597844/study-of-gelatin-as-an-effective-energy-absorbing-layer-for-laser-bioprinting
#20
Ruitong Xiong, Zhengyi Zhang, Wenxuan Chai, Douglas B Chrisey, Yong Huang
Laser-induced forward transfer printing, also commonly known as laser printing, has been widely implemented for three-dimensional bioprinting due to its unique orifice-free nature during printing. However, the printing quality has the potential to be further improved for various laser bioprinting applications. The objectives of this study are to investigate the feasibility of using gelatin as an energy absorbing layer (EAL) material for laser bioprinting and its effects on the quality of printed constructs, bioink printability, and post-printing cell viability and process-induced DNA damage...
June 9, 2017: Biofabrication
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