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Biofabrication

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https://www.readbyqxmd.com/read/29786607/pre-set-extrusion-bioprinting-for-multiscale-heterogeneous-tissue-structure-fabrication
#1
Donggu Kang, Geunseon Ahn, Donghwan Kim, Hyun-Wook Kang, Seokhwan Yun, Won-Soo Yun, Jin-Hyung Shim, Songwan Jin
Recent advances in three-dimensional (3D) bioprinting technology have led to various attempts in fabricating human tissue-like structures. However, current bioprinting technologies have limitations for creating native tissue-like structures. To resolve these issues, we developed a new pre-set extrusion bioprinting technique that can create heterogeneous, multicellular, and multimaterial structures simultaneously. The key to this ability lies in the use of a precursor cartridge that can stably preserve a multimaterial with a pre-defined configuration that can be simply embedded in a syringe-based printer head...
May 22, 2018: Biofabrication
https://www.readbyqxmd.com/read/29786602/microfluidic-system-for-modelling-3d-tumour-invasion-into-surrounding-stroma-and-drug-screening
#2
Zhichang Du, Shengli Mi, Xiaoman Yi, Yuanyuan Xu, Wei Sun
Tumour invasion into the surrounding stroma is a critical step in metastasis, and it is necessary to clarify the role of microenvironmental factors in tumour invasion. We present a microfluidic system that simulated and controlled multi-factors of the tumour microenvironment for three-dimensional (3D) assessment of tumour invasion into the stroma. The simultaneous, precise and continuous arrangement of two 3D matrices was visualised to observe the migration of cancer cell populations or single cells by transfecting cells with a fluorescent protein...
May 22, 2018: Biofabrication
https://www.readbyqxmd.com/read/29693552/bio-resin-for-high-resolution-lithography-based-biofabrication-of-complex-cell-laden-constructs
#3
Khoon Lim, Riccardo Levato, Pedro F Costa, Miguel Castilho, Cesar R Alcala-Orozco, Kim M A van Dorenmalen, Ferry P W Melchels, Debby Gawlitta, Gary J Hooper, Jos Malda, Tim B F Woodfield
Lithography-based three-dimensional (3D) printing technologies allow high spatial resolution that exceeds that of typical extrusion-based bioprinting approaches, allowing to better mimic the complex architecture of biological tissues. Additionally, lithographic printing via digital light processing (DLP) enables fabrication of free-form lattice and patterned structures which cannot be easily produced with other 3D printing approaches. While significant progress has been dedicated to the development of cell-laden bioinks for extrusion-based bioprinting, less attention has been directed towards the development of cyto-compatible bioresins and their application in lithography-based biofabrication, limiting the advancement of this promising technology...
April 25, 2018: Biofabrication
https://www.readbyqxmd.com/read/29651999/photolithographic-stereolithographic-tandem-fabrication-of-4d-smart-scaffolds-for-improved-stem-cell-cardiomyogenic-differentiation
#4
Shida Miao, Haitao Cui, Margaret Nowicki, Se-Jun Lee, Jose Almeida, Xuan Zhou, Wei Zhu, Xiaoliang Yao, Fahed Masood, Michael W Plesniak, Muhammad Mohiuddin, Lijie Grace Zhang
4D printing is a highly innovative additive manufacturing process for fabricate smart structures with the ability to transform over time. Significantly different from regular 4D printing techniques, this study focuses on creating novel 4D hierarchical micropatterns using a unique photolithographic-stereolithographic-tandem strategy (PSTS) with smart soybean oil epoxidized acrylate (SOEA) inks for effectively regulating human bone marrow mesenchymal stem cell (hMSC) cardiomyogenic behaviors. The 4D effect refers to autonomous conversion of the surficial-patterned scaffold into a pre-designed construct through an external stimulus delivered immediately after printing...
April 13, 2018: Biofabrication
https://www.readbyqxmd.com/read/29638221/self-assembled-human-osseous-cell-sheets-as-living-biopapers-for-the-laser-assisted-bioprinting-of-human-endothelial-cells
#5
Fabien Kawecki, William P Clafshenkel, Francois A Auger, Jean-Michel Bourget, Julie Fradette, Raphael Devillard
A major challenge during the engineering of voluminous bone tissues is to maintain cell viability in the central regions of the construct. In vitro prevascularization of bone substitutes relying on endothelial cell bioprinting has the potential to resolve this issue and to replicate the native bone microvasculature. Laser-assisted bioprinting (LAB) commonly uses biological layers of hydrogel, called "biopapers", to support patterns of printed cells and constitute the basic units of the construct. The self-assembly approach of tissue engineering allows the production of biomimetic cell-derived bone extracellular matrix including living cells...
April 11, 2018: Biofabrication
https://www.readbyqxmd.com/read/29637901/research-and-development-of-3d-printed-vasculature-constructs
#6
Xinda Li, Libiao Liu, Xinzhi Zhang, Tao Xu
Artificial blood vessels must be strong, flexible, and do not lead to blockage after implantation. It is therefore important to select an appropriate fabrication process for products to meet these requirements. This review discusses the current methods for making artificial blood vessels, focusing on fabrication principle, materials, and applications. Among these methods, 3D printing is very promising since it has unique capability to make complicated 3-dimensional structure with multiple types of materials, and can be completely digitalized...
April 11, 2018: Biofabrication
https://www.readbyqxmd.com/read/29578448/laser-bioprinting-of-human-induced-pluripotent-stem-cells-the-effect-of-printing-and-biomaterials-on-cell-survival-pluripotency-and-differentiation
#7
Lothar Koch, Andrea Deiwick, Annika Franke, Kristin Schwanke, Axel Haverich, Robert Zweigerdt, Boris Chichkov
Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to the patient without immunological concerns. 3D printing of hiPSCs could enable the generation of functional organs for replacement therapies or realization of organ-on-chip systems for individualized medicine. Printing of living cells was demonstrated with immortalized cell lines, primary cells, and adult stem cells with different printing technologies and biomaterials...
April 25, 2018: Biofabrication
https://www.readbyqxmd.com/read/29595143/matrix-stiffness-and-tumor-associated-macrophages-modulate-epithelial-to-mesenchymal-transition-of-human-adenocarcinoma-cells
#8
Marta Alonso-Nocelo, Theresa M Raimondo, Kyle H Vining, Rafael López-López, Maria de la Fuente, David J Mooney
The tumor microenvironment (TME) is gaining increasing attention in oncology, as it is recognized to be functionally important during tumor development and progression. Tumors are heterogeneous tissues that, in addition to tumor cells, contain tumor-associated cell types such as immune cells, fibroblasts, and endothelial cells. These other cells, together with the specific extracellular matrix (ECM), create a permissive environment for tumor growth. While the influence of tumor-infiltrating cells and mechanical properties of the ECM in tumor invasion and progression have been studied separately, their interaction within the complex TME and the epithelial -to-mesenchymal transition (EMT) is still unclear...
March 28, 2018: Biofabrication
https://www.readbyqxmd.com/read/29570458/3d-printing-strategies-for-peripheral-nerve-regeneration
#9
Eugen B Petcu, Rajiv Midha, Erin McColl, Aurel Popa-Wagner, Traian V Chirila, Paul D Dalton
After many decades of biomaterials research for peripheral nerve regeneration, a clinical product (the nerve guide), is emerging as a proven alternative for relatively short injury gaps. This review identifies aspects where 3D printing can assist in improving long-distance nerve guide regeneration strategies. These include (1) 3D printing of the customizable nerve guides, (2) fabrication of scaffolds that fill nerve guides, (3) 3D bioprinting of cells within a matrix/bioink into the nerve guide lumen and the (4) establishment of growth factor gradients along the length a nerve guide...
March 23, 2018: Biofabrication
https://www.readbyqxmd.com/read/29451128/signal-amplifying-nanoparticle-hydrogel-hybrid-microarray-biosensor-for-metal-enhanced-fluorescence-detection-of-organophosphorus-compounds
#10
Minsu Kim, Ji Eon Kwon, Kangwon Lee, Won-Gun Koh
In this study, we developed an enzyme-based miniaturized fluorescence biosensor to detect paraoxon, one of the most well-known neurotoxic organophosphorus compounds. The biosensor was fabricated with poly(ethylene glycol) (PEG) hydrogel microarrays that entrapped acetylcholinesterase (AChE) and quantum dots (QDs) as fluorescence reporters. Metal-enhanced fluorescence (MEF) was utilized to amplify the fluorescence signal, which was achieved by decorating QDs on the surface of silica-coated silver nanoparticles (Ag@Silica)...
March 16, 2018: Biofabrication
https://www.readbyqxmd.com/read/29451122/sprouting-angiogenesis-in-engineered-pseudo-islets
#11
Monika Hospodiuk, Madhuri Dey, Bugra Ayan, Donna Sosnoski, Kazim K Moncal, Yang Wu, Ibrahim T Ozbolat
Despite the recent achievements in cell-based therapies for curing type-1 diabetes (T1D), capillarization in beta (β)-cell clusters is still a major roadblock as it is essential for long-term viability and function of β-cells in vivo. In this research, we report sprouting angiogenesis in engineered pseudo islets (EPIs) made of mouse insulinoma βTC3 cells and rat heart microvascular endothelial cells (RHMVECs). Upon culturing in three-dimensional (3D) constructs under angiogenic conditions, EPIs sprouted extensive capillaries into the surrounding matrix...
March 16, 2018: Biofabrication
https://www.readbyqxmd.com/read/29417931/3d-printed-lego-%C3%A2-like-modular-microfluidic-devices-based-on-capillary-driving
#12
Jing Nie, Qing Gao, Jing-Jiang Qiu, Miao Sun, An Liu, Lei Shao, Jian-Zhong Fu, Peng Zhao, Yong He
The field of how to rapidly assemble microfluidics with modular components continuously attracts researchers' attention, however, extra efforts must be devoted to solving the problems of leaking and aligning between individual modules. This paper presents a novel type of modular microfluidic device, driven by capillary force. There is no necessity for a strict seal or special alignment, and its open structures make it easy to integrate various stents and reactants. The key rationale for this method is to print different functional modules with a low-cost three-dimensional (3D) printer, then fill the channels with capillary materials and assemble them with plugs like Lego® bricks...
March 12, 2018: Biofabrication
https://www.readbyqxmd.com/read/29460846/construction-of-a-liver-sinusoid-based-on-the-laminar-flow-on-chip-and-self-assembly-of-endothelial-cells
#13
Shengli Mi, Xiaoman Yi, Zhichang Du, Yuanyuan Xu, Wei Sun
The liver is one of the main metabolic organs, and nearly all ingested drugs will be metabolized by the liver. Only a small fraction of drugs are able to come onto the market during drug development, and hepatic toxicity is a major cause for drug failure. Since drug development is costly in both time and materials, an in vitro liver model that can accelerate bioreactions in the liver and reduce drug consumption is imperative in the pharmaceutical industry. The liver on a chip is an ideal alternative for its controllable environment and tiny size, which means constructing a more biomimetic model, reducing material consumption as well as promoting drug diffusion and reaction...
February 20, 2018: Biofabrication
https://www.readbyqxmd.com/read/29350200/leaf-templated-microwell-integrated-microfluidic-chips-for-high-throughput-cell-experiments
#14
Mao Mao, Jiankang He, Yongjie Lu, Xiao Li, Tianjiao Li, Wenxing Zhou, Dichen Li
As an alternative to conventional cell culture and animal testing, an organ-on-a-chip is applied to study the biological phenomena of organ development and disease, as well as the interactions between human tissues and external stimuli such as chemicals, forces and electricity. The pattern design of a microfluidic channel is one of the key approaches to regulate cell growth and differentiation, because these channels work as a crucial vasculature system to control the fluidic flow throughout the organ-on-a-chip device...
February 5, 2018: Biofabrication
https://www.readbyqxmd.com/read/29327690/laser-direct-writing-by-two-photon-polymerization-of-3d-honeycomb-like-structures-for-bone-regeneration
#15
Irina Alexandra Paun, Roxana Cristina Popescu, Cosmin Catalin Mustaciosu, Marian Zamfirescu, Bogdan Stefanita Calin, Mona Mihailescu, Maria Dinescu, Andrei Popescu, Diana Chioibasu, Mihai Soproniy, Catalin Romeo Luculescu
A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a random spatial arrangement and do not preserve the isotropy on the whole volume. Here, we report on the fabrication and testing of an innovative 3D hierarchical, honeycomb-like structure (HS), with reproducible and isotropic arhitecture, that allows in 'volume' migration of osteoblasts...
February 5, 2018: Biofabrication
https://www.readbyqxmd.com/read/29394158/microspheres-containing-decellularized-cartilage-induce-chondrogenesis-in-vitro-and-remain-functional-after-incorporation-within-a-poly-caprolactone-filament-useful-for-fabricating-a-3d-scaffold
#16
Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin, Patrick W Whitlock
In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS + dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. The microspheres were spherical in shape and protein encapsulation efficiency within MS + dCM was 63.4%. The sustained release of proteins from MS + dCM was observed over 4 weeks in vitro...
February 2, 2018: Biofabrication
https://www.readbyqxmd.com/read/29319535/scalable-and-physiologically-relevant-microenvironments-for-human-pluripotent-stem-cell-expansion-and-differentiation
#17
Qiang Li, Haishuang Lin, Qian Du, Kan Liu, Ou Wang, Catelyn Evans, Hannah Christian, Chi Zhang, Yuguo Lei
Human pluripotent stem cells (hPSCs) are required in large numbers for various biomedical applications. However, the scalable and cost-effective culturing of high quality hPSCs and their derivatives remains very challenging. Here, we report a novel and physiologically relevant 3D culture system (called the AlgTube cell culture system) for hPSC expansion and differentiation. With this system, cells are processed into and cultured in microscale alginate hydrogel tubes that are suspended in the cell culture medium in a culture vessel...
February 1, 2018: Biofabrication
https://www.readbyqxmd.com/read/29360631/proof-of-concept-3d-bioprinting-of-pigmented-human-skin-constructs
#18
Wei Long Ng, Jovina Tan Zhi Qi, Wai Yee Yeong, May Win Naing
Three-dimensional (3D) pigmented human skin constructs have been fabricated using a 3D bioprinting approach. The 3D pigmented human skin constructs are obtained from using three different types of skin cells (keratinocytes, melanocytes and fibroblasts from three different skin donors) and they exhibit similar constitutive pigmentation (pale pigmentation) as the skin donors. A two-step drop-on-demand bioprinting strategy facilitates the deposition of cell droplets to emulate the epidermal melanin units (pre-defined patterning of keratinocytes and melanocytes at the desired positions) and manipulation of the microenvironment to fabricate 3D biomimetic hierarchical porous structures found in native skin tissue...
January 23, 2018: Biofabrication
https://www.readbyqxmd.com/read/29337695/automated-fabrication-of-photopatterned-gelatin-hydrogels-for-organ-on-chips-applications
#19
Janna C Nawroth, Lisa L Scudder, Ryan T Halvorson, Jason Tresback, John P Ferrier, Sean P Sheehy, Alex Cho, Suraj Kannan, Ilona Sunyovszki, Josue A Goss, Patrick H Campbell, Kevin Kit Parker
Organ-on-chip platforms aim to improve preclinical models for organ-level responses to novel drug compounds. Heart-on-a-chip assays in particular require tissue engineering techniques that rely on labor-intensive photolithographic fabrication or resolution-limited 3D printing of micropatterned substrates, which limits turnover and flexibility of prototyping. We present a rapid and automated method for large scale on-demand micropatterning of gelatin hydrogels for organ-on-chip applications using a novel biocompatible laser-etching approach...
January 16, 2018: Biofabrication
https://www.readbyqxmd.com/read/29235445/direct-three-dimensional-printing-of-polymeric-scaffolds-with-nanofibrous-topography
#20
Aruna Prasopthum, Kevin M Shakesheff, Jing Yang
Three-dimensional (3D) printing is a powerful manufacturing tool for making 3D structures with well-defined architectures for a wide range of applications. The field of tissue engineering has also adopted this technology to fabricate scaffolds for tissue regeneration. The ability to control architecture of scaffolds, e.g. matching anatomical shapes and having defined pore size, has since been improved significantly. However, the material surface of these scaffolds is smooth and does not resemble that found in natural extracellular matrix (ECM), in particular, the nanofibrous morphology of collagen...
January 12, 2018: Biofabrication
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