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Biofabrication

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https://www.readbyqxmd.com/read/29199637/automated-3d-bioassembly-of-micro-tissues-for-biofabrication-of-hybrid-tissue-engineered-constructs
#1
Naveen Vijayan Mekhileri, Khoon Lim, Gabriella C J Brown, Isha Mutreja, Ben S Schon, Gary J Hooper, Tim B F Woodfield
Bottom-up biofabrication approaches combining micro-tissue fabrication techniques with extrusion-based 3D printing of thermoplastic polymer scaffolds are emerging strategies in tissue engineering. These biofabrication strategies support native self-assembly mechanisms observed in developmental stages of tissue or organoid growth as well as promoting cell-cell interactions and cell differentiation capacity. Few technologies have been developed to automate the precise assembly of micro-tissues or tissue modules into structural scaffolds...
December 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/29176036/one-step-delivery-of-a-functional-multi-layered-cell-sheet-using-a-thermally-expandable-hydrogel-with-controlled-presentation-of-cell-adhesive-proteins
#2
Yu Bin Lee, Joong-Yup Lee, Ha Yeon Byun, Taufiq Ahmad, Mitsuru Akashi, Michiya Matsusaki, Heungsoo Shin
In this study, we developed a new system enabling rapid delivery of a multi-layered cell sheet by combining layer-by-layer (LBL) coating of a cell membrane and surface engineered thermally expandable hydrogel. Human dermal fibroblasts were LBL-coated with fibronectin (FN) and gelatin to form a multi-layered cell sheet in a single seeding step via spontaneous 3D cell-cell interactions. FN was covalently immobilized onto the surface of a Tetronic®-based hydrogel at two different concentrations (1 and 5 g/ml) for stable adhesion of the multi-layered cell sheet, followed by polydopamine (PD) coating...
November 27, 2017: Biofabrication
https://www.readbyqxmd.com/read/29176035/coaxial-extrusion-bioprinting-of-3d-microfibrous-constructs-with-cell-favorable-gelatin-methacryloyl-microenvironments
#3
Wanjun Liu, Zhe Zhong, Ning Hu, Yixiao Zhou, Lucia Maggio, Amir Miri, Alessio Fragasso, Xiangyu Jin, Ali Khademhosseini, Yu Shrike Zhang
Bioinks with shear-thinning/rapid solidification properties and strong mechanics are usually needed for the bioprinting of three-dimensional (3D) cell-laden constructs. As such, it remains challenging to generate soft constructs from bioinks at low concentrations that are favorable for cellular activities. Herein, we report a strategy to fabricate cell-laden constructs with tunable 3D microenvironments achieved by bioprinting of gelatin methacryloyl (GelMA)/alginate core/sheath microfibers, where the alginate sheath serves as a template to support and confine the GelMA pre-hydrogel in the core during the extrusion process, allowing for subsequent UV crosslinking...
November 27, 2017: Biofabrication
https://www.readbyqxmd.com/read/29148982/a-dentin-derived-hydrogel-bioink-for-3d-printing-of-cell-laden-scaffolds-for-regenerative-dentistry
#4
Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane Miranda Franca, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz Bertassoni
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential...
November 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/29106379/networked-concave-microwell-arrays-for-constructing-3-d-cell-spheroids
#5
Geon Hui Lee, JaeSeo Lee, Gi-Hun Lee, WooYoul Joung, Soo-Hyun Kim, Sang-Hoon Lee, Joong Yull Park, Dong-Hwee Kim
The engineered three-dimensional (3-D) cell cultivation system for the production of multicellular spheroids has attracted considerable attention due to its improved in vivo relevance to cellular communications compared to the traditional two-dimensional (2-D) cell culture platform. The formation and maintenance of cell spheroids in healthy condition is the critical factor for tissue engineering applications such as the repair of damaged tissues, the development of organ replacement parts, and preclinical drug tests...
November 6, 2017: Biofabrication
https://www.readbyqxmd.com/read/29083312/bioprinted-chitosan-gelatin-thermosensitive-hydrogels-using-an-inexpensive-3d-printer
#6
Kevin D Roehm, Sundararajan V Madihally
The primary bottleneck in bioprinting cell-laden structures with carefully controlled spatial relation is a lack of biocompatible inks and printing conditions. In this regard, we explored using thermogelling chitosan-gelatin (CG) hydrogel as a novel bioprinting ink; CG hydrogels is unique in that it undergoes a spontaneous phase change at physiological temperature, and does not need post-processing. In addition, we used a low cost (<$800) compact 3D printer, and modified with a new extruder to print using disposable syringes and hypodermic needles...
October 30, 2017: Biofabrication
https://www.readbyqxmd.com/read/29058681/optimization-of-binding-b-lymphocytes-in-a-microfluidic-channel-surface-modification-stasis-time-and-shear-response
#7
Scott Charles McCormick, Ziqiu Tong, Angela Ivask, Mariana Morozesk, Nico Voelcker, Enzo Lombi, Craig Priest
Binding and maintaining cells inside microfluidic channels is a challenging task due to the potential release of cells from the channels with the flow and accompanying shear stress. In this work we optimized the binding of human B-lymphocyte cells (HR1K) inside a microfluidic channel and determined the strength of this binding under shear stress of flowing liquid. In order to determine the parameters required for a live/dead test in microfluidic devices, populations of both living and dead cells were tested separately...
October 23, 2017: Biofabrication
https://www.readbyqxmd.com/read/29045238/hypergravity-induced-multicellular-spheroid-generation-with-different-morphological-patterns-precisely-controlled-on-a-centrifugal-microfluidic-platform
#8
Jiheum Park, Gi-Hun Lee, Joong Yull Park, Jung Chan Lee, Hee Chan Kim
In living tissue, cells exist in three-dimensional (3D) microenvironments with intricate cell-cell interactions. To model these cellular environments, numerous techniques for generating cell spheroids have been proposed and improved. However, previously reported methods still have limitations in uniformity, reproducibility, scalability, through-put, etc. Here, we present a centrifugal microfluidic-based spheroid (CMS) formation method for generating both co-culture and mono-culture 3D spheroids in a highly controlled manner...
October 18, 2017: Biofabrication
https://www.readbyqxmd.com/read/29019465/facile-and-controllable-electrochemical-fabrication-of-cell-adhesive-polypyrrole-electrodes-using-pyrrole-rgd-peptides
#9
Lindy K Jang, Semin Kim, Jiwon Seo, Jae Young Lee
Electrically conductive polymers, such as polypyrrole (PPy), have been widely used for the fabrication of various biosensors and tissue engineering scaffolds. For their biologically relevant applications, conductive biomaterials capable of intimate cellular interactions are highly desired. However, conventional methods to incorporate biomolecules into conductive polymers do not offer fine and easy control over the surface density of the biomolecules and/or their stability. We present a novel method to electrochemically immobilize cell adhesive Arg-Gly-Asp (RGD) ligands on PPy electrode surfaces with a simple control over the peptide surface density by varying the electrodeposition time...
October 11, 2017: Biofabrication
https://www.readbyqxmd.com/read/28990579/bone-matrix-production-in-hydroxyapatite-modified-hydrogels-suitable-for-bone-bioprinting
#10
Annika Wenz, Kirsten Borchers, Günter E M Tovar, Petra Kluger
Though bioprinting is a forward-looking approach in bone tissue engineering, the development of bioinks which are on the one hand processable with the chosen printing technique, and on the other hand possess the relevant mechanical as well as osteoconductive features remains a challenge. In the present study, polymer solutions based on methacrylated gelatin and methacrylated hyaluronic acid modified with hydroxyapatite particles (5 wt%) were prepared. Encapsulation of primary human adipose-derived stem cells in the hydroxyapatite-containing gels and culture for 28 days resulted in a storage moduli significantly increased to126...
October 9, 2017: Biofabrication
https://www.readbyqxmd.com/read/28976366/recombinant-spider-silk-based-bioinks
#11
Elise DeSimone, Kristin Schacht, Alexandra Pellert, Thomas Scheibel
Bioinks, 3D cell culture systems which can be printed, are still in the early development stages. Currently, extensive research is going into designing printers to be more accommodating to bioinks, designing scaffolds with stiff materials as support structures for the often soft bioinks, and modifying the bioinks themselves. Recombinant spider silk proteins, a potential biomaterial component for bioinks, have high biocompatibility, can be processed into several morphologies and can be modified with cell adhesion motifs to enhance their bioactivity...
October 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28976364/assessing-bioink-shape-fidelity-to-aid-material-development-in-3d-bioprinting
#12
Alexandre Ribeiro, Maarten Michiel Blokzijl, Riccardo Levato, Claas Willem Visser, Miguel Castilho, Wim E Hennink, Tina Vermonden, Jos Malda
During extrusion-based bioprinting, the deposited bioink filaments are subjected to deformations, such as collapse of overhanging filaments, which compromises the ability to stack several layers of bioink, and fusion between adjacent filaments, which compromises the resolution and maintenance of a desired pore structure. When developing new bioinks, approaches to assess their shape fidelity after printing would be beneficial to evaluate the degree of deformation of the deposited filament and to estimate how similar the final printed construct would be to the design...
October 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28976359/automated-fabrication-of-hydrogel-microfibers-with-tunable-diameters-for-controlled-cell-alignment
#13
You Yang, Xiaolu Liu, Dan Wei, Meiling Zhong, Jing Sun, Likun Guo, Hongsong Fan, Xingdong Zhang
A newly designed spinning device was utilized to produce continuous hydrogel microfibers with tunable diameters. It was found that the diameter of microfiber was dependent on perfusion speed and coagulation wheel rotation rate. Their correlation was finally described by a mathematical expression and proved to be useful for a size-tunable spinning technique. Based on the controllable fabrication of hydrogel microfibers with desired size, microfiber/cell size-dependent cellular orientated spreading was studied by using PC12 and L929 as model cells...
October 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28976356/creating-hierarchical-porosity-hydroxyapatite-scaffold-with-osteoinduction-by-three-dimensional-printing-and-microwave-sintering
#14
Xuan Pei, Liang Ma, Boqing Zhang, Jianxun Sun, Yong Sun, Yujiang Fan, Zhongru Gou, Changchun Zhou, Xingdong Zhang
Hierarchical porosity, which includes micropores and macropores in scaffolds, contributes to important multiple biological functions for tissue regeneration. This paper introduced a two-step method of combining three-dimensional printing (3DP) and microwave sintering to fabricate two-level hierarchical porous scaffolds. The results showed that 3D printing made the macroporous structure well-controlled and microwave sintering generated micropores on macropore surface. The resulting hierarchical macro-/micro-porous hydroxyapatite scaffold induced bone formation following intramuscular implantation...
October 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28975895/pla-short-i-sub-micron-fibers-i-reinforcement-of-3d-bioprinted-alginate-constructs-for-cartilage-regeneration
#15
Alicja Kosik-Kozioł, Marco Costantini, Tomasz Bolek, Krisztina Szoke, Andrea Barbetta, Jan E Brinchmann, Wojciech Święszkowski
In this study, we present an innovative strategy to reinforce 3D printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1.7 kPa) can be increased threefold (up to 25.1 ± 3.8 kPa) with the addition of PLA short fibers. Furthermore, to assess the performance of such materials in cartilage tissue engineering, we loaded the bioinks with human chondrocytes and cultured in vitro the bioprinted constructs for up to 14 days...
October 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28930091/proposal-to-assess-printability-of-bioinks-for-extrusion-based-bioprinting-and-evaluation-of-rheological-properties-governing-bioprintability
#16
Naomi Claire Paxton, Willi Smolan, Thomas Böck, Ferry P W Melchels, Juergen Groll, Tomasz Juengst
The development and formulation of printable inks for extrusion-based 3D bioprinting has been a major challenge in the field of biofabrication. Inks, often polymer solutions with the addition of crosslinking to form hydrogels, must not only display adequate mechanical properties for the chosen application, but also show high biocompatibility as well as printability. Here we describe a reproducible two-step method for the assessment of the printability of inks for bioprinting, focussing firstly on screening ink formulations to assess fibre formation and the ability to form 3D constructs before presenting a method for the rheological evaluation of inks to characterise the yield point, shear thinning and recovery behaviour...
September 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/28930090/biomimetic-matrix-fabricated-by-lmp-1-gene-transduced-mc3t3-e1-cells-for-bone-regeneration
#17
Junxuan Ma, Wei Guo, Manman Gao, Baoding Huang, Qihua Qi, Zemin Ling, Yan Chen, Hao Hu, Hailian Zhou, Fei Yu, Ke Chen, Zhiyu Zhou, Deming Xiao, Xuenong Zou
Bone healing is regulated by multiple microenvironmental signals provided by the extracellular matrix (ECM). The study aimed to mimic native osteoinductive microenvironment by developing an ECM using gene-transduced cells. LIM mineralization protein-1 (LMP-1) gene was transferred to the murine pre-osteoblast cells (MC3T3-E1) using lentiviral vectors. Western blotting essay indicated that the MC3T3-E1 cells expressed an increased level of bone morphologic protein-2, -4 and-7 (BMP-2, -4 and -7) after LMP-1 gene transduction...
September 20, 2017: Biofabrication
https://www.readbyqxmd.com/read/29190216/networked-concave-microwell-arrays-for-constructing-3d-cell-spheroids
#18
Geon Hui Lee, Jae Seo Lee, Gi-Hun Lee, Woo Youl Joung, Soo Hyun Kim, Sang Hoon Lee, Joong Yull Park, Dong-Hwee Kim
The engineered three-dimensional (3D) cell cultivation system for the production of multicellular spheroids has attracted considerable attention due to its improved in vivo relevance to cellular communications compared with the traditional two-dimensional (2D) cell culture platform. The formation and maintenance of cell spheroids in a healthy condition is the critical factor for tissue engineering applications such as the repair of damaged tissues, the development of organ replacement parts and preclinical drug tests...
November 30, 2017: Biofabrication
https://www.readbyqxmd.com/read/29134949/pla-short-sub-micron-fiber-reinforcement-of-3d-bioprinted-alginate-constructs-for-cartilage-regeneration
#19
Alicja Kosik-Kozioł, Marco Costantini, Tomasz Bolek, Krisztina Szöke, Andrea Barbetta, Jan Brinchmann, Wojciech Święszkowski
In this study, we present an innovative strategy to reinforce 3D-printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1.7 kPa) can be increased threefold (up to 25.1 ± 3.8 kPa) with the addition of PLA short fibers. Furthermore, to assess the performance of such materials in cartilage tissue engineering, we loaded the bioinks with human chondrocytes and cultured in vitro the bioprinted constructs for up to 14 days...
November 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/29134948/effect-of-scaffold-morphology-and-cell-co-culture-on-tenogenic-differentiation-of-hadmsc-on-centrifugal-melt-electrospun-poly-l%C3%A2-lactic-acid-fibrous-meshes
#20
Shaohua Wu, Hao Peng, Xiuhong Li, Philipp N Streubel, Yong Liu, Bin Duan
Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. The effects of CME processing parameters, including rotational speed, voltage, and temperature, on fiber properties (i...
November 14, 2017: Biofabrication
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