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Wenfang Li, Xueyan Hu, Shuaitao Yang, Shuping Wang, Chenghong Zhang, Hai Wang, Yuen Yee Cheng, Yiwei Wang, Tianqing Liu, Kedong Song
Cancer biology and drug discovery are heavily dependent on conventional 2D cell culture systems. However, 2D culture is significantly limited by its ability to reflect 'true biology' of tumor in vivo. Three-dimensional (3D) in vitro cell culture models have been introduced to aid cancer drug discovery by better modeling tumor microenvironment. Here, decellularized lung scaffold cultured with MCF-7 cancer cells were bioengineered as a platform to study tumor development and anti-cancer drug evaluation. Excellent cell compatibility of decellularized lung scaffolds promoted cell growth and proliferation...
September 19, 2018: Biofabrication
Bin Wang, Xiaolong Tu, Jin Wei, Li Wang, Yong Chen
Substrate elasticity regulates cell functions including cell aggregation and stem cell differentiation. The ability to manufacture substrates of desired elasticity over a broad range is therefore crucial for both fundamental research and advanced applications. In this work, we developed a method to fabricate dense elastomer pillars of different heights on a rigid substrate, providing an effective elasticity ranging from 3 to 168 kPa. Assisted with an elastomer stencil of honeycomb pattern for cell seeding, we obtained uniform colonies of human induced pluripotent stem cells (hiPSCs) and differentiated cardiomyocytes on the pillar substrates of different modulus...
September 12, 2018: Biofabrication
Dalila Petta, Angela R Armiento, Dirk Grijpma, Mauro Alini, David Eglin, Matteo D'Este
Extrusion-based three-dimensional bioprinting relies on bioinks engineered to combine viscoelastic properties for extrusion and shape retention, and biological properties for cytocompatibility and tissue regeneration. To satisfy these conflicting requirements, bioinks often utilize either complex mixtures or complex modifications of biopolymers. In this paper we introduce and characterize a bioink exploiting a dual crosslinking mechanism, where an enzymatic reaction forms a soft gel suitable for cell encapsulation and extrusion, while a visible light photo-crosslinking allows shape retention of the printed construct...
September 6, 2018: Biofabrication
Minghao Nie, Shoji Takeuchi
Nature builds living organisms in a bottom-up fashion, starting from the expression of genetic information on a cellular level, to the proliferation, differentiation, and self-assembly of cells into tissues/organs during embryo development and wound-healing processes. To mimic this bottom-up approach, it is essential to handle and manipulate small-scale biomaterials using specific technologies, such as microfluidic techniques. Microfluidics provides the tool-sets that deal with the behavior, precise control and manipulation of small amounts of fluids...
September 17, 2018: Biofabrication
Y Pang, S S Mao, R Yao, J Y He, Z Z Zhou, L Feng, K T Zhang, S J Cheng, W Sun
An advanced in vitro cervical tumor model was established by 3D printing to study the epithelial-to-mesenchymal transition (EMT), which is a very important stage of dissemination of carcinoma leading to metastatic tumors. A HeLa/hydrogel grid construct composed of gelatin, alginate, Matrigel and HeLa cells was fabricated by forced extrusion in a layer-by-layer fashion. HeLa cells rapidly proliferated, formed spheroids and presented tumorigenic characteristic in the 3D-printed structure. With the supplement of TGF-β, aggregated HeLa cells started to disintegrate, and some of them changed into fibroblast-like spindle morphology, which indicated that EMT was induced...
September 10, 2018: Biofabrication
Shinji Sakai, Kei Mochizuki, Yanfei Qu, Matthew Mail, Masaki Nakahata, Masahito Taya
Hydrogels were prepared by contacting air containing 10-50 ppm H2 O2 with an aqueous solution containing polymer(s) possessing phenolic hydroxyl (Ph) moieties (polymer-Ph) and horseradish peroxidase (HRP). In this system, HRP catalyzes cross-linking of the Ph moieties by consuming H2 O2 diffused from the air. The hydrogelation rate and mechanical properties of the resultant hydrogels can be tuned by controlling the H2 O2 concentration in air, the exposure time of the air containing H2 O2 to the solution containing polymer-Phs and HRP, and the HRP concentration...
September 5, 2018: Biofabrication
Carmine Onofrillo, Serena Duchi, Cathal D O'Connell, Romane Blanchard, Andrea J O'Connor, Mark Scott, Gordon G Wallace, Peter F M Choong, Claudia Di Bella
Cartilage injuries cause pain and loss of function, and if severe may result in osteoarthritis (OA). 3D bioprinting is now a tangible option for the delivery of bioscaffolds capable of regenerating the deficient cartilage tissue. Our team has developed a handheld device, the Biopen, to allow in situ additive manufacturing during surgery. Given its ability to extrude in a core/shell manner, the Biopen can preserve cell viability during the biofabrication process, and it is currently the only biofabrication tool tested as a surgical instrument in a sheep model using homologous stem cells...
August 21, 2018: Biofabrication
Giuseppe Criscenti, Carmelo De Maria, Alessia Longoni, Clemens A van Blitterswijk, Hugo A M Fernandes, Giovanni Vozzi, Lorenzo Moroni
The fabrication of bioactive scaffolds able to mimic the in vivo cellular microenvironment is a challenge for regenerative medicine. The creation of sites for the selective binding of specific endogenous proteins represents an attractive strategy to fabricate scaffolds able to elicit specific cell response. Here, electrospinning (ESP) and soft-molecular imprinting (soft-MI) techniques were combined to fabricate a soft-molecular imprinted electrospun bioactive scaffold (SMIES) for tissue regeneration. Scaffolds functionalized using different proteins and growth factors (GFs) arranged onto the surface were designed, fabricated and validated with different polyesters, demonstrating the versatility of the developed approach...
August 20, 2018: Biofabrication
Seth Polk, Nardos Sori, Nick Thayer, Nathan Kemper, Yas Maghdouri-White, Anna A Bulysheva, Michael P Francis
INTRODUCTION: Current collagen fiber manufacturing methods for biomedical applications, such as electrospinning and extrusion, have had limited success in clinical translation, partially due to scalability, cost, and complexity challenges. Here we explore an alternative, simplified and scalable collagen fiber formation method, termed 'pneumatospinning,' to generate submicron collagen fibers from benign solvents. METHODS AND RESULTS: Clinical grade type I atelocollagen from calf corium was electrospun or pneumatospun as sheets of aligned and isotropic fibrous scaffolds...
August 14, 2018: Biofabrication
Kideok Kim, Sung-Hwan Kim, Gi-Hun Lee, Joong Yull Park
A cell spheroid culture has the benefit of simulating in vivo three-dimensional cell environments. Microwell systems have been developed to mass-produce large quantities of uniform spheroids, and are frequently used in research areas, such as cell biology, anticancer drug development, and regenerative therapy. Recently reported concave-bottomed microwell systems have delivered more benefits in producing spheroids of higher quality and facilitating more effective research. However, microwell fabrication methods are often complicated or expensive, and there are inherent limitations in the functions and characteristics of existing microwells...
August 14, 2018: Biofabrication
Tilman Ahlfeld, Falko Doberenz, David Kilian, Corina Vater, Paula Korn, Günter Lauer, Anja Lode, Michael Gelinsky
Due to their characteristic resemblance of the mineral component of bone, calcium phosphates are widely accepted as optimal bone substitute materials. Recent research focused on the development of pasty calcium phosphate cement (CPC) formulations, which can be fabricated into various shapes by low-temperature extrusion-based additive manufacturing, namely 3D plotting. While it could be demonstrated that sensitive substances like growth factors can be integrated in such printed CPC scaffolds without impairment of their biological activity live cells cannot be suspended in CPC as they may not be functional when enclosed in a solid and stiff matrix...
July 27, 2018: Biofabrication
Hyeonjun Hong, Man-Il Huh, Sang Min Park, Kyoung-Pil Lee, Hong Kyun Kim, Dong Sung Kim
Recently, compressed collagen has attracted much attention as a potential alternative for a limbal epithelial stem cell (LESC) carrier to treat limbal stem cell deficiency (LSCD), in that it can provide mechanically improved collagen fibrillar structures compared to conventional collagen hydrogel. However, its clinical efficacy as an LESC carrier has not yet been studied through in vivo transplantation due to limited mechanical strength that cannot withstand a force induced by surgical suturing and low resistance to enzymatic degradation...
July 23, 2018: Biofabrication
Yang Li, Xulin Jiang, Ling Li, Zhi-Nan Chen, Ge Gao, Rui Yao, Wei Sun
Human induced pluripotent stem cells (hiPSCs) are more likely to successfully avoid the immunological rejection and ethical problems that are often encountered by human embryonic stem cells in various stem cell studies and applications. To transfer hiPSCs from the laboratory to clinical applications, researchers must obtain sufficient cell numbers. In this study, 3D cell printing was used as a novel method for iPSC scalable expansion. Hydroxypropyl chitin (HPCH), utilized as a new type of bioink, and a set of optimized printing parameters were shown to achieve high cell survival (>90%) after the printing process and high proliferation efficiency (∼32...
July 12, 2018: Biofabrication
Teng Gao, Gregory J Gillispie, Joshua S Copus, Anil Kumar Pr, Young-Joon Seol, Anthony Atala, James J Yoo, Sang Jin Lee
Three-dimensional bioprinting has emerged as a promising technique in tissue engineering applications through the precise deposition of cells and biomaterials in a layer-by-layer fashion. However, the limited availability of hydrogel bioinks is frequently cited as a major issue for the advancement of cell-based extrusion bioprinting technologies. It is well known that highly viscous materials maintain their structure better, but also have decreased cell viability due to the higher forces which are required for extrusion...
June 29, 2018: Biofabrication
Liqun Ning, Haoying Sun, Tiphanie Lelong, Romain Guilloteau, Ning Zhu, David J Schreyer, Xiongbiao Chen
Three-dimensional bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve regeneration, yet remain unachievable. This paper presents our study on bioprinting Schwann cell-encapsulated scaffolds using composite hydrogels of alginate, fibrin, hyaluronic acid, and/or RGD peptide, for nerve tissue engineering applications...
June 29, 2018: Biofabrication
Lokesh Karthik Narayanan, Trevor L Thompson, Rohan A Shirwaiker, Binil Starly
Biofabrication processes can affect biological quality attributes of encapsulated cells within constructs. Currently, assessment of the fabricated constructs is performed offline by subjecting the constructs to destructive assays that require staining and sectioning. This drawback limits the translation of biofabrication processes to industrial practice. In this work, we investigate the dielectric response of viable cells encapsulated in bioprinted 3D hydrogel constructs to an applied alternating electric field as a label-free non-destructive monitoring approach...
June 28, 2018: Biofabrication
Stephen W Sawyer, Shivkumar Vishnempet Shridhar, Kairui Zhang, Lucas D Albrecht, Alex B Filip, Jason A Horton, Pranav Soman
Despite the promise of stem cell engineering and the new advances in bioprinting technologies, one of the major challenges in the manufacturing of large scale bone tissue scaffolds is the inability to perfuse nutrients throughout thick constructs. Here, we report a scalable method to create thick, perfusable bone constructs using a combination of cell-laden hydrogels and a 3D printed sacrificial polymer. Osteoblast-like Saos-2 cells were encapsulated within a gelatin methacrylate (GelMA) hydrogel and 3D printed polyvinyl alcohol pipes were used to create perfusable channels...
June 28, 2018: Biofabrication
Jennifer Gansau, Lara Kelly, Conor Timothy Buckley
Cell delivery and leakage during injection remains a challenge for cell-based intervertebral disc regeneration strategies. Cellular microencapsulation may offer a promising approach to overcome these limitations by providing a protective niche during intradiscal injection. Electrohydrodynamic spraying (EHDS) is a versatile one-step approach for microencapsulation of cells using a high voltage electric field. The primary objective of this work was to characterise key processing parameters such as applied voltage (0, 5, 10 or 15 kV), emitter needle gauge (21, 26 or 30 G), alginate concentration (1%, 2% or 3%) and flow rate (50, 100, 250 or 500 μl min-1 ) to regulate the size and morphology of alginate microcapsules as well as subsequent cell viability when altering these parameters...
June 25, 2018: Biofabrication
M Yan, P L Lewis, R N Shah
3D-printing has expanded our ability to produce reproducible and more complex scaffold architectures for tissue engineering applications. In order to enhance the biological response within these 3D-printed scaffolds incorporating nanostructural features and/or specific biological signaling may be an effective means to optimize tissue regeneration. Peptides amphiphiles (PAs) are a versatile supramolecular biomaterial with tailorable nanostructural and biochemical features. PAs are widely used in tissue engineering applications such as angiogenesis, neurogenesis, and bone regeneration...
June 18, 2018: Biofabrication
Vladislav A Parfenov, Elizaveta V Koudan, Elena A Bulanova, Pavel A Karalkin, Frederico DAS Pereira, Nikita E Norkin, Alisa D Knyazeva, Anna A Gryadunova, Oleg F Petrov, Mikhail M Vasiliev, Maxim I Myasnikov, Valery P Chernikov, Vladimir A Kasyanov, Artem Yu Marchenkov, Kenn Brakke, Yusef D Khesuani, Utkan Demirci, Vladimir A Mironov
Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape and capable to fusion have been biofabricated from primary sheep chondrocytes using non-adhesive technology...
June 18, 2018: Biofabrication
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