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Biofabrication

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https://www.readbyqxmd.com/read/28504242/simultaneous-microfluidic-spinning-of-multiple-strands-of-submicron-fiber-for-the-production-of-free-standing-porous-membranes-for-biological-application
#1
DoYeun Park, JiSoo Park, Heeyeong Jang, Jie Cheng, Soo-Hyun Kim, Sang-Hoon Lee
Microfibers produced using electrospinning and microfluidics-based technologies have been developed as a powerful tool in tissue engineering applications such as drug delivery and scaffolds. The applications of these fibers, however, have been limited because of the hazardous solvents used to make them, difficulties in controlling the pore sizes of their membrane forms, and downscaling the size of the fiber. Nevertheless, extending the use of these fibers such as the production of a free-standing porous membrane appropriate for cell-based research is highly needed for tissue engineering, organ-on-a-chip, and drug delivery research and applications...
May 15, 2017: Biofabrication
https://www.readbyqxmd.com/read/28488594/large-scale-production-and-controlled-deposition-of-single-huvec-spheroids-for-bioprinting-applications
#2
Ludwig Gutzweiler, Sabrina Kartmann, Kevin Troendle, Leo Benning, Günter Finkenzeller, Roland Zengerle, Peter Koltay, Bjoern Stark, Stefan Zimmermann
We present 1.) a fast and automated method for large scale production of HUVEC spheroids based on the hanging drop method and 2.) a novel method for well-controlled lateral deposition of single spheroids by drop-on-demand printing. Large scale spheroid production is achieved via printing 1536 droplets of HUVEC cell suspension having a volume of 1 µl each within 3 minutes at a pitch of 2.3 mm within an array of 48 x 32 droplets onto a flat substrate. Printing efficiencies between 97.9% and 100% and plating efficiencies between 87...
May 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28488588/leaf-inspired-microcontact-printing-vascular-patterns
#3
Lian Wong, John Pegan, Basia Gabela-Zuniga, Michelle Khine, Kara E McCloskey
The vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft. A number of strategies are currently being investigated including very promising microfluidics systems. Here, we explored the potential for generating a vasculature-patterned endothelial cells (EC) that could be integrated into distinct layers between sheets of primary cells. Bioinspired from the leaf veins, we generated a reverse mold with a fractal vascular-branching pattern that models the unique spatial arrangement over multiple length scales that precisely mimic branching vasculature...
May 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28485303/synthesis-of-cell-composite-alginate-microfibers-by-microfluidics-with-the-application-potential-of-small-diameter-vascular-grafts
#4
Mingying Liu, Zhizhi Zhou, Yongchao Chai, Shuai Zhang, Xiaomeng Wu, Shaopeng Huang, Jiaqiang Su, Jiahuan Jiang
Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application remains is still challenging. In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the methods of microfluidics were considered original. A simple glass microfluidic device with a "L-shape" cylindrical-flow channel in the microfluidic platform was developed. The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow respectively...
May 9, 2017: Biofabrication
https://www.readbyqxmd.com/read/28485302/cell-patterning-via-laser-micro-nano-structured-silicon-surfaces
#5
Christina Yiannakou, Chara Simitzi, Aleka Manousaki, Costas Fotakis, Anthi Ranella, Emmanuel Stratakis
The surface topography of biomaterials can have an important impact on the cellular adhesion, growth and proliferation. Apart from the overall roughness, the detailed morphological features at all length scales significantly affect the cell-biomaterial interactions in a plethora of applications including structural implants, tissue engineering scaffolds and biosensors. In this study, we present a simple, one-step direct laser patterning technique to fabricate nanoripples and dual-rough hierarchical micro/nano structures to control SW10 cell attachment and migration...
May 9, 2017: Biofabrication
https://www.readbyqxmd.com/read/28485301/developing-organ-on-a-chip-concepts-using-bio-mechatronic-design-methodology
#6
Jonas Christoffersson, Danny van van Noort, C-F Mandenius
Mechatronic design is an engineering methodology for conceiving, configuring, and optimising the design of a technical device or product to the needs and requirements of the final user. In this article, we show how the basic principles of this methodology can be exploited for in vitro cell cultures - often referred to as organ-on-a-chip devices. Due to the key role of the biological cells, we have introduced the term bio-mechatronic design, to highlight the complexity of designing a system that should integrate biology, mechanics and electronics in the same device structure...
May 9, 2017: Biofabrication
https://www.readbyqxmd.com/read/28471354/introducing-biomimetic-shear-and-ion-gradients-to-microfluidic-spinning-improves-silk-fiber-strength
#7
David Li, Matthew Jacobsen, Nae Rim, Daniel Backman, David Kaplan, Joyce Y Wong
Silkworm silk is an attractive biopolymer for biomedical applications due to its high mechanical strength and biocompatibility; as a result, there is increasing interest in scalable devices to spin silk and recombinant silk so as to improve and customize their properties for diverse biomedical purposes.[1] While artificial spinning of regenerated silk fibroins adds tunability to properties such as degradation rate and surface functionalization, the resulting fibers do not yet approach the mechanical strength of native silkworm silk...
May 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28467316/the-fabrication-of-double-layered-chitosan-gelatin-genipin-nanosphere-coating-for-sequential-and-controlled-release-of-therapeutic-proteins
#8
Ruiying Chen, Xinjie Cai, Kena Ma, Yi Zhou, Yining Wang, Tao Jiang
Bone regeneration is a complicated process and includes a number of distinct and sequential stages of coordinated cellular actions under the regulation of multiple growth factors. Therefore, bone grafting materials in which growth factors can be incorporated and released in a programmed order in line with the bone tissue healing process may lead to desirable clinical outcomes. In the present study, a double-layered chitosan/gelatin/genipin (d-CSG/G) nanosphere coating is developed by using layer-by-layer electrophoretic deposition (EPD) and genipin crosslinking...
May 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28462910/optimum-silicon-doping-of-carbon-coatings-by-pulsed-laser-technique-for-superior-functional-biomedical-surfaces-fabrication
#9
Ion N Mihailescu, Dorota Bociaga, Gianina Popescu-Pelin, George Stan, Liviu Duta, Gabriel Socol, Carmen Chifiriuc, Coralia Bleotu, Veronica Lazar, Marius-Adrian Husanu, Irina Zgura, Florin Miculescu, Irina Negut, Claudiu Hapenciuc
We report on the fabrication of Silicon (Si) doped Carbon (C) [C:Si] structures by combinatorial pulsed laser deposition to identify the best design for a novel generation of multi-functional coated implants. The synthesized films were characterized from the morphological, structural, compositional, mechanical and microbiological points of view. Scanning Electron Microscopy investigation highlighted the presence, on top of the deposited layers, of spheroid particulates with sizes in the micron range. No micro-cracks or delaminations were observed...
May 2, 2017: Biofabrication
https://www.readbyqxmd.com/read/28462906/biomimetic-design-and-fabrication-of-multilayered-osteochondral-scaffolds-by-low-temperature-deposition-manufacturing-and-thermal-induced-phase-separation-techniques
#10
Ting Zhang, Hefeng Zhang, Laquan Zhang, Shuaijun Jia, Jian Liu, Zhuo Xiong, Wei Sun
Integrative osteochondral repair is a useful strategy for cartilage-defect repair. To mimic the microenvironment, it is necessary that scaffolds effectively mimic the extracellular matrix of natural cartilage and subchondral bone. In this study, biomimetic osteochondral scaffolds containing an oriented cartilage layer, a compact layer, and a three-dimensional (3D)-printed core-sheath structured-bone layer were developed. The oriented cartilage layer was designed to mimic the structural and material characteristics of native cartilage tissue and was fabricated with cartilage matrix-chitosan materials, using thermal-induced phase-separation technology...
May 2, 2017: Biofabrication
https://www.readbyqxmd.com/read/28436920/hybrid-3d-printing-and-electrodeposition-approach-for-controllable-3d-alginate-hydrogel-formation
#11
Wanfeng Shang, Yanting Liu, Wenfeng Wan, Chengzhi Hu, Zeyang Liu, Chin To Wong, Toshio Fukuda, Yajing Shen
Calcium alginate hydrogel is one of the most widely used biocompatible materials in biomedical and tissue engineering. This paper reports a hybrid 3D printing and electrodeposition approach to form the 3D calcium alginate hydrogel in a controllable manner. Firstly, a specific 3D printing system is developed by combining a 3D printer and a customized ejection syringe. Then, the mixed solution (1% w/v sodium alginate and 0.25% w/v CaCO3 nano particles) is filled in the syringe and ejected out from the nozzle to the substrate continuously...
April 24, 2017: Biofabrication
https://www.readbyqxmd.com/read/28393759/laser-technique-for-the-fabrication-of-blood-vessels-like-models-for-preclinical-studies-of-pathologies-under-flow-conditions
#12
María Aymerich, Ezequiel Álvarez, Carmen Bao, Isabel Moscoso, José Ramón González-Juanatey, Maria Flores Arias
In this work a method for fabricating functionalized preclinical devices is presented. The manufacturing process combines a laser indirect writing technique to fabricate a soda-lime glass master and soft-lithography methods to obtain the final structure in polydimethylsiloxane (PDMS). The roughness of the device is modified in a controlled manner by applying a post-thermal treatment to the master and devices with different roughness values are created. The PDMS devices are fully covered with human umbilical vein cells (HUVEC) in a two-step process...
April 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28382923/a-compliant-and-biomimetic-three-layered-vascular-graft-for-small-blood-vessels
#13
Yi Zhang, Xiangshun Li, Géraldine Guex, Shanshan Liu, Eike Müller, Riccardo Innocenti Malini, Huijing Zhao, Markus Rottmar, Katharina Maniura, Rene Rossi, Fabrizio Spano
Engineering a small diameter vascular graft with mechanical and biological properties comparable to living tissues remains challenging. Often, current devices lead to thrombosis and unsatisfactory long-term patency as a result of poor blood compatibility and a mismatch between the mechanical properties of the living tissue and the implanted biomaterial. Address-ing all these requirements is essential to produce scaffolds able to survive throughout the life of the patient. For this purpose, we fabricated a novel three-layered vascular graft by combining electrospinning and braiding...
April 6, 2017: Biofabrication
https://www.readbyqxmd.com/read/28374682/biofabricated-soft-network-composites-for-cartilage-tissue-engineering
#14
Onur Bas, Elena De-Juan-Pardo, Christoph Meinert, Davide D'Angella, Jeremy Baldwin, Laura Bray, R Wellard, Stefan Kollmannsberger, Ernst Rank, Carsten Werner, Travis Klein, Isabelle Catelas, Dietmar Werner Hutmacher
Articular cartilage from a material science point of view is a soft network composite that plays a critical role in load-bearing joints during dynamic loading. Its composite structure, consisting of a collagen fiber network and a hydrated proteoglycan matrix, gives rise to the complex mechanical properties of the tissue including viscoelasticity and stress relaxation. Melt Electrospinning Writing (MEW) allows the design and fabrication of medical grade polycaprolactone (mPCL) fibrous networks for the reinforcement of soft hydrogel matrices for cartilage tissue engineering...
April 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/28300041/surface-micromorphology-of-cross-linked-tetrafunctional-polylactide-scaffolds-inducing-vessel-growth-and-bone-formation
#15
Daria Kuznetsova, Aleksey Ageykin, Anastasia Koroleva, Andrea Deiwick, Anastasia Shpichka, Anna Solovieva, Sergey Kostjuk, Aleksandra Meleshina, Svetlana Rodimova, Anastasia Akovanceva, Denis Butnaru, Anastasia Frolova, Elena Zagaynova, Boris Chichkov, Victor Bagratashvili, Peter Timashev
In the presented study, we have developed a synthetic strategy allowing a gradual variation of a polylactide arms' length, which later influences the micromorphology of the scaffold surface, formed by a two-photon polymerization technique. It has been demonstrated that the highest number of cells is present on the scaffolds with the roughest surface made of the polylactide with longer arms (PLA760), and osteogenic differentiation of mesenchymal stem cells is most pronounced on such scaffolds. According to the results of biological testing, the PLA760 scaffolds were implanted into a created cranial defect in a mouse for an in vivo assessment of the bone tissue formation...
March 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28548045/3d-liver-membrane-system-by-co-culturing-human-hepatocytes-sinusoidal-endothelial-and-stellate-cells
#16
Haysam Mohamed Magdy Ahmed, Simona Salerno, Sabrina Morelli, Lidetta Giorno, Loredana De Bartolo
In this study, a designed approach has been utilized for the development of a 3D liver system. This approach makes use of primary human sinusoidal endothelial cells, stellate cells and hepatocytes that are seeded sequentially on hollow fiber membranes (HF) in order to mimic the layers of cells found in vivo. To this purpose modified polyethersulfone (PES) HF membranes were used for the creation of a 3D human liver system in static and dynamic conditions. In order to verify the positive effect of non-parenchymal cells on the maintenance of hepatocyte viability and functions, homotypic cultures of hepatocytes alone on the HF membranes were further investigated...
May 26, 2017: Biofabrication
https://www.readbyqxmd.com/read/28530207/fabrication-of-biomimetic-bone-grafts-with-multi-material-3d-printing
#17
Nicholas Sears, Prachi Dhavalikar, Michael Whitely, Elizabeth Cosgriff-Hernandez
Extrusion deposition is a versatile method for the 3D printing of biomaterials such as hydrogels, ceramics, and suspensions. Recently, a new class of emulsion inks were developed that can be used to create tunable, hierarchically porous materials with a cure-on-dispense method. Propylene fumarate dimethacrylate (PFDMA) was selected to fabricate bone grafts using this technology due to its established biocompatibility, osteoconductivity, and good compressive properties. Scaffolds fabricated from PFDMA emulsion inks displayed compressive modulus and yield strength of approximately 15 and 1 MPa, respectively...
May 22, 2017: Biofabrication
https://www.readbyqxmd.com/read/28516893/design-of-electrohydrodynamic-sprayed-polyethylene-glycol-hydrogel-microspheres-for-cell-encapsulation
#18
Anisa S Qayyum, Era Jain, Grant Kolar, Yonghyun Kim, Scott A Sell, Silviya P Zustiak
Electrohydrodynamic spraying (EHS) has recently gained popularity for microencapsulation of cells for applications in cell delivery and tissue engineering. Some of the polymers compatible with EHS are alginate, chitosan, and other similar natural polymers, which are subject to ionotropic or physical gelation. It is desirable to further extend the use of the EHS technique beyond such polymers for wider biofabrication applications. Here, building upon our previous work of making PEG microspheres via EHS, we utilized the principles of EHS to fabricate cell-laden polyethylene glycol (PEG) hydrogel microspheres...
May 18, 2017: Biofabrication
https://www.readbyqxmd.com/read/28513477/microtube-array-membrane-bioreactor-promotes-neuronal-differentiation-and-orientation
#19
Sabrina Morelli, Antonella Piscioneri, Simona Salerno, Chien-Chung Chen, Chee Ho Chew, Lidietta Giorno, Enrico Drioli, Loredana De Bartolo
An important challenge in neuronal tissue engineering is to create innovative tools capable of promoting cellular response in terms of neuronal differentiation and neurite orientation that may be used as investigational platforms for studying neurobiological events and neurodegenerative disorders. A novel membrane bioreactor was created to provide a 3D well-controlled microenvironment for neuronal outgrowth. The bioreactor consisted of poly-L-lactic acid highly aligned microtube array (PLLA-MTA) membranes assembled in parallel within a chamber that establish an intraluminal and an extraluminal compartment whose communication occurs through the pores of the MTA membrane walls...
May 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/28493850/importance-of-endogenous-extracellular-matrix-in-biomechanical-properties-of-human-skin-model
#20
Flavien Pillet, Laure Gibot, Moinecha Madi, Marie-Pierre Rols, Etienne Dague
The physical and mechanical properties of cells modulate their behavior such proliferation rate, migration and extracellular matrix remodeling. In order to study cell behavior in a tissue-like environment in vitro, it is of utmost importance to develop biologically and physically relevant 3D cell models. Here, we characterized the physical properties of a single cell type growing in configurations of increasing complexity. From one human skin biopsy, primary dermal fibroblasts were isolated and seeded to give monolayer (2D model), spheroid (3D model poor in extracellular matrix) and tissue-engineered cell sheet (3D model rich in endogenous extracellular matrix)...
May 11, 2017: Biofabrication
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