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Biofabrication

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https://www.readbyqxmd.com/read/29417931/3d-printed-lego%C3%A2-like-modular-microfluidic-devices-based-on-capillary-driving
#1
Jing Nie, Qing Gao, Jing-Jiang Qiu, Miao Sun, An Liu, Lei Shao, Jianzhong Fu, Peng Zhao, Yong He
The field of how to rapidly assemble microfluidics with modular components continuously attracts researchers' attention, however extra efforts must be devoted to solving the problems of leaking and aligning between individual modules. This paper presents a novel type of modular microfluidic device, driven by capillary force. There is no necessity for a strict seal or special alignment, and its open structures make it easy to integrate various stents and reactants. The key rationale for this method is to print different functional modules with a low-cost three-dimensional (3D) printer, then fill the channels with capillary materials and assemble them with plugs like Lego® bricks...
February 8, 2018: Biofabrication
https://www.readbyqxmd.com/read/29350200/leaf-templated-microwell-integrated-microfluidic-chips-for-high-throughput-cell-experiments
#2
Mao Mao, Jiankang He, Yongjie Lu, Xiao Li, Tianjiao Li, Wenxing Zhou, Dichen Li
As an alternative to conventional cell culture and animal testing, organ-on-a-chip is applied to study the biological phenomena of organ development and disease, as well as the interactions between human tissues and external stimuli such as chemicals, forces and electricity. The pattern design of microfluidic channel is one of the key approaches to regulate cell growth and differentiation, because these channels work as a crucial vasculature system to control the fluidic flow throughout the organ-on-a-chip device...
January 19, 2018: Biofabrication
https://www.readbyqxmd.com/read/29327690/laser-direct-writing-by-two-photon-polymerization-of-3d-honeycomb-like-structures-for-bone-regeneration
#3
Irina Alexandra Paun, Roxana Cristina Popescu, Cosmin Catalin Mustaciosu, Marian Zamfirescu, Bogdan Stefanita Calin, Mona Mihailescu, Maria Dinescu, Andrei Popescu, Diana Georgiana Chioibasu, Mihai Sopronyi, Catalin Luculescu
A major limitation of existing 3D implantable structures for bone tissue engineering is that most of the cells rapidly attach on the outer edges of the structure, restricting the cells penetration into the inner parts and causing the formation of a necrotic core. Furthermore, these structures generally possess a random spatial arrangement and do not preserve the isotropy on the whole volume. Here, we report on the fabrication and testing of an innovative 3D hierarchical, honeycomb-like structure (HS), with reproducible and isotropic arhitecture, that allows "in volume" migration of osteoblasts...
January 12, 2018: Biofabrication
https://www.readbyqxmd.com/read/29319535/scalable-and-physiologically-relevant-microenvironments-for-human-pluripotent-stem-cell-expansion-and-differentiation
#4
Qiang Li, Haishuang Lin, Qian Du, Kan Liu, Ou Wang, Catelyn Antionette Evans, Hannah Marie Christian, Chi Zhang, Yuguo Lei
Human pluripotent stem cells (hPSCs) are required in large numbers for various biomedical applications. However, the scalable and cost-effective culturing of high quality hPSCs and their derivatives remains very challenging. Here, we report a novel and physiologically relevant 3D culture system (called the AlgTube cell culture system) for hPSC expansion and differentiation. With this system, cells are processed into and cultured in microscale alginate hydrogel tubes that are suspended in the cell culture medium in a culture vessel...
January 10, 2018: Biofabrication
https://www.readbyqxmd.com/read/29313825/microspheres-containing-decellularized-cartilage-induce-chondrogenesis-i-in-vitro-i-and-remain-functional-after-incorporation-within-a-poly-caprolactone-filament-useful-for-fabricating-a-3d-scaffold
#5
Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin, Patrick Whitlock
In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS+dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. The microspheres were spherical in shape and protein encapsulation efficiency within MS+dCM was 63.4%. The sustained release of proteins from MS+dCM was observed over 4 weeks in vitro. Both MS+dCM and MS(-) were cytocompatible...
January 9, 2018: Biofabrication
https://www.readbyqxmd.com/read/29235445/direct-three-dimensional-printing-of-polymeric-scaffolds-with-nanofibrous-topography
#6
Aruna Prasopthum, Kevin Shakesheff, Jing Yang
Three-dimensional (3D) printing is a powerful manufacturing tool for making 3D structures with well-defined architectures for a wide range of applications. The field of tissue engineering has also adopted this technology to fabricate scaffolds for tissue regeneration. The ability to control architecture of scaffolds, e.g. matching anatomical shapes and having defined pore size, has since been improved significantly. However, the material surface of these scaffolds is smooth and does not resemble that found in natural extracellular matrix (ECM), in particular, the nanofibrous morphology of collagen...
December 13, 2017: Biofabrication
https://www.readbyqxmd.com/read/29235444/contact-guidance-for-cardiac-tissue-engineering-using-3d-bioprinted-gelatin-patterned-hydrogel
#7
Ajay Tijore, Scott Alexander Irvine, Udi Sarig, Priyadarshini Mhaisalkar, Vrushali Baisane, Subbu S Venkatraman
Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes (CMs) contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the alignment, elongation, and differentiation of hMSC. Notably, the cells displayed well defined F-actin anisotropy and elongated morphology on the microchanneled hydrogel, hence showing the effects of topographical control over cell behavior...
December 13, 2017: Biofabrication
https://www.readbyqxmd.com/read/29199637/automated-3d-bioassembly-of-micro-tissues-for-biofabrication-of-hybrid-tissue-engineered-constructs
#8
Naveen Vijayan Mekhileri, Khoon Lim, Gabriella C J Brown, Isha Mutreja, Ben S Schon, Gary J Hooper, Tim B F Woodfield
Bottom-up biofabrication approaches combining micro-tissue fabrication techniques with extrusion-based 3D printing of thermoplastic polymer scaffolds are emerging strategies in tissue engineering. These biofabrication strategies support native self-assembly mechanisms observed in developmental stages of tissue or organoid growth as well as promoting cell-cell interactions and cell differentiation capacity. Few technologies have been developed to automate the precise assembly of micro-tissues or tissue modules into structural scaffolds...
December 4, 2017: Biofabrication
https://www.readbyqxmd.com/read/29176036/one-step-delivery-of-a-functional-multi-layered-cell-sheet-using-a-thermally-expandable-hydrogel-with-controlled-presentation-of-cell-adhesive-proteins
#9
Yu Bin Lee, Joong-Yup Lee, Ha Yeon Byun, Taufiq Ahmad, Mitsuru Akashi, Michiya Matsusaki, Heungsoo Shin
In this study, we developed a new system enabling rapid delivery of a multi-layered cell sheet by combining layer-by-layer (LBL) coating of a cell membrane and surface engineered thermally expandable hydrogel. Human dermal fibroblasts were LBL-coated with fibronectin (FN) and gelatin to form a multi-layered cell sheet in a single seeding step via spontaneous 3D cell-cell interactions. FN was covalently immobilized onto the surface of a Tetronic®-based hydrogel at two different concentrations (1 and 5 g/ml) for stable adhesion of the multi-layered cell sheet, followed by polydopamine (PD) coating...
November 27, 2017: Biofabrication
https://www.readbyqxmd.com/read/29176035/coaxial-extrusion-bioprinting-of-3d-microfibrous-constructs-with-cell-favorable-gelatin-methacryloyl-microenvironments
#10
Wanjun Liu, Zhe Zhong, Ning Hu, Yixiao Zhou, Lucia Maggio, Amir Miri, Alessio Fragasso, Xiangyu Jin, Ali Khademhosseini, Yu Shrike Zhang
Bioinks with shear-thinning/rapid solidification properties and strong mechanics are usually needed for the bioprinting of three-dimensional (3D) cell-laden constructs. As such, it remains challenging to generate soft constructs from bioinks at low concentrations that are favorable for cellular activities. Herein, we report a strategy to fabricate cell-laden constructs with tunable 3D microenvironments achieved by bioprinting of gelatin methacryloyl (GelMA)/alginate core/sheath microfibers, where the alginate sheath serves as a template to support and confine the GelMA pre-hydrogel in the core during the extrusion process, allowing for subsequent UV crosslinking...
November 27, 2017: Biofabrication
https://www.readbyqxmd.com/read/29394158/microspheres-containing-decellularized-cartilage-induce-chondrogenesis-in-vitro-and-remain-functional-after-incorporation-within-a-poly-caprolactone-filament-useful-for-fabricating-a-3d-scaffold
#11
Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin, Patrick W Whitlock
In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS + dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. The microspheres were spherical in shape and protein encapsulation efficiency within MS + dCM was 63.4%. The sustained release of proteins from MS + dCM was observed over 4 weeks in vitro...
February 2, 2018: Biofabrication
https://www.readbyqxmd.com/read/29360631/proof-of-concept-3d-bioprinting-of-pigmented-human-skin-constructs
#12
Wei Long Ng, Jovina Tan Zhi Qi, Wai Yee Yeong, May Win Naing
Three-dimensional (3D) pigmented human skin constructs have been fabricated using a 3D bioprinting approach. The 3D pigmented human skin constructs are obtained from using three different types of skin cells (keratinocytes, melanocytes and fibroblasts from three different skin donors) and they exhibit similar constitutive pigmentation (pale pigmentation) as the skin donors. A two-step drop-on-demand bioprinting strategy facilitates the deposition of cell droplets to emulate the epidermal melanin units (pre-defined patterning of keratinocytes and melanocytes at the desired positions) and manipulation of the microenvironment to fabricate 3D biomimetic hierarchical porous structures found in native skin tissue...
January 23, 2018: Biofabrication
https://www.readbyqxmd.com/read/29337695/automated-fabrication-of-photopatterned-gelatin-hydrogels-for-organ-on-chips-applications
#13
Janna C Nawroth, Lisa L Scudder, Ryan T Halvorson, Jason Tresback, John P Ferrier, Sean P Sheehy, Alex Cho, Suraj Kannan, Ilona Sunyovszki, Josue A Goss, Patrick H Campbell, Kevin Kit Parker
Organ-on-chip platforms aim to improve preclinical models for organ-level responses to novel drug compounds. Heart-on-a-chip assays in particular require tissue engineering techniques that rely on labor-intensive photolithographic fabrication or resolution-limited 3D printing of micropatterned substrates, which limits turnover and flexibility of prototyping. We present a rapid and automated method for large scale on-demand micropatterning of gelatin hydrogels for organ-on-chip applications using a novel biocompatible laser-etching approach...
January 16, 2018: Biofabrication
https://www.readbyqxmd.com/read/29320372/a-dentin-derived-hydrogel-bioink-for-3d-bioprinting-of-cell-laden-scaffolds-for-regenerative-dentistry
#14
Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane M França, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz E Bertassoni
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential...
January 10, 2018: Biofabrication
https://www.readbyqxmd.com/read/29190216/networked-concave-microwell-arrays-for-constructing-3d-cell-spheroids
#15
Geon Hui Lee, Jae Seo Lee, Gi-Hun Lee, Woo Youl Joung, Soo Hyun Kim, Sang Hoon Lee, Joong Yull Park, Dong-Hwee Kim
The engineered three-dimensional (3D) cell cultivation system for the production of multicellular spheroids has attracted considerable attention due to its improved in vivo relevance to cellular communications compared with the traditional two-dimensional (2D) cell culture platform. The formation and maintenance of cell spheroids in a healthy condition is the critical factor for tissue engineering applications such as the repair of damaged tissues, the development of organ replacement parts and preclinical drug tests...
November 30, 2017: Biofabrication
https://www.readbyqxmd.com/read/29148982/a-dentin-derived-hydrogel-bioink-for-3d-printing-of-cell-laden-scaffolds-for-regenerative-dentistry
#16
Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane Miranda Franca, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz Bertassoni
Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential...
November 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/29134949/pla-short-sub-micron-fiber-reinforcement-of-3d-bioprinted-alginate-constructs-for-cartilage-regeneration
#17
Alicja Kosik-Kozioł, Marco Costantini, Tomasz Bolek, Krisztina Szöke, Andrea Barbetta, Jan Brinchmann, Wojciech Święszkowski
In this study, we present an innovative strategy to reinforce 3D-printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1.7 kPa) can be increased threefold (up to 25.1 ± 3.8 kPa) with the addition of PLA short fibers. Furthermore, to assess the performance of such materials in cartilage tissue engineering, we loaded the bioinks with human chondrocytes and cultured in vitro the bioprinted constructs for up to 14 days...
November 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/29134948/effect-of-scaffold-morphology-and-cell-co-culture-on-tenogenic-differentiation-of-hadmsc-on-centrifugal-melt-electrospun-poly-l%C3%A2-lactic-acid-fibrous-meshes
#18
Shaohua Wu, Hao Peng, Xiuhong Li, Philipp N Streubel, Yong Liu, Bin Duan
Engineered tendon grafts offer a promising alternative for grafting during the reconstruction of complex tendon tears. The tissue-engineered tendon substitutes have the advantage of increased biosafety and the option to customize their biochemical and biophysical properties to promote tendon regeneration. In this study, we developed a novel centrifugal melt electrospinning (CME) technique, with the goal of optimizing the fabrication parameters to generate fibrous scaffolds for tendon tissue engineering. The effects of CME processing parameters, including rotational speed, voltage, and temperature, on fiber properties (i...
November 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/29106379/networked-concave-microwell-arrays-for-constructing-3-d-cell-spheroids
#19
Geon Hui Lee, JaeSeo Lee, Gi-Hun Lee, WooYoul Joung, Soo-Hyun Kim, Sang-Hoon Lee, Joong Yull Park, Dong-Hwee Kim
The engineered three-dimensional (3-D) cell cultivation system for the production of multicellular spheroids has attracted considerable attention due to its improved in vivo relevance to cellular communications compared to the traditional two-dimensional (2-D) cell culture platform. The formation and maintenance of cell spheroids in healthy condition is the critical factor for tissue engineering applications such as the repair of damaged tissues, the development of organ replacement parts, and preclinical drug tests...
November 6, 2017: Biofabrication
https://www.readbyqxmd.com/read/29083312/bioprinted-chitosan-gelatin-thermosensitive-hydrogels-using-an-inexpensive-3d-printer
#20
Kevin D Roehm, Sundararajan V Madihally
The primary bottleneck in bioprinting cell-laden structures with carefully controlled spatial relation is a lack of biocompatible inks and printing conditions. In this regard, we explored using thermogelling chitosan-gelatin (CG) hydrogel as a novel bioprinting ink; CG hydrogels is unique in that it undergoes a spontaneous phase change at physiological temperature, and does not need post-processing. In addition, we used a low cost (<$800) compact 3D printer, and modified with a new extruder to print using disposable syringes and hypodermic needles...
October 30, 2017: Biofabrication
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