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Biofabrication

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https://www.readbyqxmd.com/read/28224971/biofabrication-of-bundles-of-poly-lactic-acid-collagen-blends-mimicking-the-fascicles-of-the-human-achille-tendon
#1
Alberto Sensini, Chiara Gualandi, Luca Cristofolini, Gianluca Tozzi, Manuela Dicarlo, Gabriella Teti, Monica Mattioli-Belmonte, Maria Letizia Focarete
Electrospinning is a promising technique for the production of scaffolds aimed at the regeneration of soft tissues. The aim of this work was to develop electrospun bundles mimicking the architecture and mechanical properties of the fascicles of the human Achille tendon. Two different blends of poly(L-lactic acid) (PLLA) and collagen (Coll) were tested, PLLA/Coll-75/25 and PLLA/Coll-50/50, and compared with bundles of pure PLLA. First, a complete physico-chemical characterization was performed on non-woven mats made of randomly arranged fibers...
February 22, 2017: Biofabrication
https://www.readbyqxmd.com/read/28211365/converging-biofabrication-and-organoid-technologies-the-next-frontier-in-hepatic-and-intestinal-tissue-engineering
#2
Kerstin Schneeberger, Bart Spee, Pedro Costa, Norman Sachs, Hans Clevers, Jos Malda
Adult tissue stem cells can form self-organizing 3D organoids in vitro. Organoids resemble small units of their organ of origin and have great potential for tissue engineering, as well as models of disease. However, current culture technology limits the size, architecture and complexity of organoids. Here, we review the establishment of intestinal and hepatic organoids and discuss how the convergence of organoids and biofabrication technologies can help overcome current limitations, and thereby further advance the translational application of organoids in tissue engineering and regenerative medicine...
February 17, 2017: Biofabrication
https://www.readbyqxmd.com/read/28169834/development-of-biomimetic-micro-patterned-device-incorporated-with-neurotrophic-gradient-and-supportive-schwann-cells-for-the-applications-in-neural-tissue-engineering
#3
Chia-Wei Yeh, Li-Wen Wang, Hsi-Chin Wu, Yi-Kong Hsieh, Jane Wang, Ming-Hong Chen, Tzu-Wei Wang
In these years, the artificial nerve guidance conduit has been developed as an alternative way to repair peripheral nerve injury. Unlike autologous nerve graft, the artificial nerve guidance conduit without proper stimulating factors and guidance cues still cannot obtain satisfactory prognosis for clinical patients. In this study, a biodegradable polymer-based implantable device has been developed and characterized. By incorporating three stimulating factors: 1) micro-patterned surface that can directionally guide the axon as physical cue; 2) neurotrophic gradient membrane that can continually attract axon outgrowth from the proximal to distal stump as chemical cue; 3) Schwann cells that can support the growth of neurite and form myelin sheath around axon as biological cue, we expect that this construct can be used as a promising nerve guidance conduit for peripheral nerve regeneration...
February 7, 2017: Biofabrication
https://www.readbyqxmd.com/read/27973346/3d-bioprinting-of-gelma-scaffolds-triggers-mineral-deposition-by-primary-human-osteoblasts
#4
Christine McBeth, Jasmin Lauer, Michael Ottersbach, Jennifer Campbell, Andre Sharon, Alexis Sauer-Budge
Due to its relatively low level of antigenicity and high durability, titanium has successfully been used as the major material for biological implants. However, because the typical interface between titanium and tissue precludes adequate transmission of load into the surrounding bone, over time, load-bearing implants tend to loosen and revision surgeries are required. Osseointegration of titanium implants requires presentation of both biological and mechanical cues that promote attachment of and trigger mineral deposition by osteoblasts...
December 14, 2016: Biofabrication
https://www.readbyqxmd.com/read/28222045/validation-of-scaffold-design-optimization-in-bone-tissue-engineering-finite-element-modeling-versus-designed-experiments
#5
Nicholas Uth, Jens Mueller, Byran Smucker, Azizeh-Mitra Yousefi
This study reports the development of biological/synthetic scaffolds for bone tissue engineering (TE) via 3D bioplotting. These scaffolds were composed of poly(L-lactic-co-glycolic acid) (PLGA), type I collagen, and nano-hydroxyapatite (nHA) in an attempt to mimic the extracellular matrix of bone. The solvent used for processing the scaffolds was 1,1,1,3,3,3-hexafluoro-2-propanol. The produced scaffolds were characterized by scanning electron microscopy, microcomputed tomography, thermogravimetric analysis, and unconfined compression test...
February 21, 2017: Biofabrication
https://www.readbyqxmd.com/read/28222044/endocrine-system-on-chip-for-a-diabetes-treatment-model
#6
Dao Thi Thuy Nguyen, Danny van Noort, In-Kyung Jeong, Sungsu Park
The endocrine system is a collection of glands producing hormones which, among others, regulates metabolism, growth and development. One important group of endocrine diseases is diabetes, which is caused by a deficiency or diminished effectiveness of endogenous insulin. By using a microfluidic perfused 3D cell-culture chip, we developed an 'endocrine system on chip' to potentially be able to screen drugs for the treatment of diabetes by measuring insulin release over time. Insulin-secreting β-cells are located in the pancreas, while L-cells, located in the small intestines, stimulate insulin secretion...
February 21, 2017: Biofabrication
https://www.readbyqxmd.com/read/28140346/increased-lipid-accumulation-and-adipogenic-gene-expression-of-adipocytes-in-3d-bioprinted-nanocellulose-scaffolds
#7
I Henriksson, P Gatenholm, D A Hägg
Compared to standard 2D culture systems, new methods for 3D cell culture of adipocytes could provide more physiologically accurate data and a deeper understanding of metabolic diseases such as diabetes. By resuspending living cells in a bioink of nanocellulose and hyaluronic acid, we were able to print 3D scaffolds with uniform cell distribution. After one week in culture, cell viability was 95%, and after two weeks the cells displayed a more mature phenotype with larger lipid droplets than standard 2D cultured cells...
February 21, 2017: Biofabrication
https://www.readbyqxmd.com/read/28195834/surface-acoustic-waves-induced-micropatterning-of-cells-in-gelatin-methacryloyl-gelma-hydrogels
#8
Shahid M Naseer, Amir Manbachi, Mohamadmahdi Samandari, Philipp Walch, Yuan Gao, Yu Shrike Zhang, Farideh Davoudi, Wesley Wang, Karen Abrinia, Jonathan M Cooper, Ali Khademhosseini, Su Ryon Shin
Acoustic force patterning is an emerging technology that provides a platform to control the spatial location of cells in a rapid, accurate, yet contactless manner. However, very few studies have been reported on the usage of acoustic force patterning for the rapid arrangement of biological objects, such as cells, in a three-dimensional (3D) environment. In this study, we report on a bio-acoustic force patterning technique, which uses surface acoustic waves (SAWs) for the rapid arrangement of cells within an extracellular matrix-based hydrogel such as gelatin methacryloyl (GelMA)...
February 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28140360/assembling-of-electrospun-meshes-into-three-dimensional-porous-scaffolds-for-bone-repair
#9
Juqing Song, Guanglin Zhu, Lin Wang, Geng An, Xuetao Shi, Yingjun Wang
Technical limitations of traditional electrospinning make it hard to produce three-dimensional (3D) scaffolds with hierarchical pore structures. Here, porous polycaprolactone (PCL) nanofiber meshes with different nano-hydroxyapatite (nHA) concentrations were prepared by electrospinning with stainless steel mesh as the collector, and 3D porous nanofiber scaffolds were fabricated via layer-by-layer assembly with a special binder (18% PCL/DCM solution). The single layer nanofiber mesh possessed very regular morphology with a hollow structure, and the nHA was not only embedded in the nanofiber but also exposed on the surfaces of the fiber, resulting in the improved surface chemical properties...
February 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28140345/ultrathin-transparent-membranes-for-cellular-barrier-and-co-culture-models
#10
Robert N Carter, Stephanie M Casillo, Andrea R Mazzocchi, Jon-Paul S DesOrmeaux, James A Roussie, Thomas R Gaborski
Typical in vitro barrier and co-culture models rely upon thick semi-permeable polymeric membranes that physically separate two compartments. Polymeric track-etched membranes, while permeable to small molecules, are far from physiological with respect to physical interactions with co-cultured cells and are not compatible with high-resolution imaging due to light scattering and autofluorescence. Here we report on an optically transparent ultrathin membrane with porosity exceeding 20%. We optimize deposition and annealing conditions to create a tensile and robust porous silicon dioxide membrane that is comparable in thickness to the vascular basement membrane (100-300 nm)...
February 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28140336/cell-like-features-imprinted-in-the-physical-nano-and-micro-topography-of-the-environment-modify-the-responses-to-anti-cancer-drugs-of-endometrial-cancer-cells
#11
Li Hui Tan, Peter H Sykes, Maan M Alkaisi, John J Evans
Topographical features of cells at nanometre resolution were fabricated in polystyrene. The study investigated the effect of physical topography on the response of cancer cells to the common anticancer drugs, paclitaxel and doxorubicin. Human endometrial cancer cells (Ishikawa) were incubated on substrates containing cell-like features that had been fabricated using our bioimprint methodology to create moulds of cells with positive (convex) and negative (concave) topography. Control cultures were performed on flat substrates...
February 14, 2017: Biofabrication
https://www.readbyqxmd.com/read/28000609/an-in-vitro-3d-model-using-collagen-coated-gelatin-nanofibers-for-studying-breast-cancer-metastasis
#12
G Janani, Mamatha M Pillai, R Selvakumar, Amitava Bhattacharyya, C Sabarinath
The study of breast cancer metastasis is limited due to poor knowledge of molecular progression of breast tumor and varied heterogeneity. For a better understanding of tumor metastasis, a reliable 3D in vitro model bridging the gap between 2D cultures and in vivo animal model studies is essential. Our study is focused on two key points: (i) designing a 3D microenvironment for studying metastasis and (ii) simulating the metastasis milieu by inducing epithelial to mesenchymal transition (EMT) and mesenchymal to epithelial transition (MET)...
February 7, 2017: Biofabrication
https://www.readbyqxmd.com/read/28071591/high-throughput-micropatterning-of-interspersed-cell-arrays-using-capillary-assembly
#13
François-Damien Delapierre, Guillaume Mottet, Vélan Taniga, Julie Boisselier, Jean-Louis Viovy, Laurent Malaquin
A novel technology is reported to immobilize different types of particles or cells on a surface at predefined positions with a micrometric precision. The process uses capillary assembly on arrays of crescent-shaped structures with different orientations. Sequential assemblies in different substrate orientations with different types of particles allow for the creation of imbricated and multiplexed arrays. In this work up to four different types of particles were deterministically localized on a surface. Using this process, antibody coated microparticles were assembled on substrates and used as capture patterns for the creation of complex cell networks...
February 6, 2017: Biofabrication
https://www.readbyqxmd.com/read/28155851/robust-tissue-growth-and-angiogenesis-in-large-sized-scaffold-by-reducing-h2o2-mediated-oxidative-stress
#14
Girdhari Rijal, Byoung Soo Kim, Falguni Pati, Dong-Heon Ha, Sung Won Kim, Dong-Woo Cho
The implantation of cell-seeded large-sized scaffold often results in insufficient tissue regeneration, which is still a challenge for successful grafting. Excess hydrogen peroxide (H2O2) released by cells propagates oxidative stress, which is the primary cause of tissue injury leading to failure in tissue regeneration. Hence, preventing tissue from oxidative damage becomes imperative. For the first time, we entrapped catalase, an antioxidant in a scaffold as a novel approach in bioengineering to prevent tissue from H2O2-induced damage...
February 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28155845/design-and-fabrication-of-a-scalable-liver-lobule-on-a-chip-microphysiological-platform
#15
Amin A Banaeiyan, Jannick Theobald, Jurgita Paukštyte, Stefan Wölfl, Caroline B Adiels, Mattias Goksör
The design and fabrication of a very large-scale liver-lobule (VLSLL)-on-a-chip device, providing a microphysiological niche for hepatocytes, is described. The device consists of an integrated network of liver-lobule-like hexagonal tissue-culture chambers constructed in a hybrid layout with a separate seed-feed network. As a key feature, each chamber contains a central outlet mimicking the central vein of a liver lobule. Separating chamber walls located between the culture area and feed network protects cells from the shear force of the convective flow...
February 3, 2017: Biofabrication
https://www.readbyqxmd.com/read/28092639/lithographic-patterning-of-antibodies-by-direct-lift-off-and-improved-surface-adhesion
#16
Sankalp Verma, Vivek Verma
The inherent property of antibodies binding to their antigen with high specificity makes them a strong candidate for sensing and detection applications. Microscale patterning of antibodies is desired for the miniaturization of sensors and fundamental cell biology studies. However, existing methodologies to pattern antibodies at the microscale are multi-step. In this work, we demonstrate microscale patterning of antibodies on a glass coverslip in a single step photolithography process. The microscale features of the photoresist were generated on the coverslip using photolithography, and the antibody solution was incubated...
January 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28092631/optoelectrofluidic-printing-system-for-fabricating-hydrogel-sheets-with-on-demand-patterned-cells-and-microparticles
#17
Hyun Ji Gi, Dongsik Han, Je-Kyun Park
This paper presents a novel optoelectrofluidic printing system that facilitates not only the optoelectrofluidic patterning of microparticles and mammalian cells but also the harvesting of the patterned microparticles encapsulated within poly(ethylene glycol) dicarylate (PEGDA) hydrogel sheets. Although optoelectrofluidic technology has numerous advantages for programmable and on-demand patterning and the feasibility of manipulating single microparticles, practical applications using existing laboratory infrastructure in biological and clinical research fields have been strictly restricted due to the impossibility of recovering the final patterned product...
January 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28071597/three-dimensional-printing-of-a-microneedle-array-on-personalized-curved-surfaces-for-dual-pronged-treatment-of-trigger-finger
#18
Seng Han Lim, Jian Yao Ng, Lifeng Kang
The hand function of patients who suffer from trigger finger can be impaired by the use of traditional splints. There is also a risk of systemic side effects with oral non-steroidal anti-inflammatory drugs (NSAIDs) used for pain relief. Microneedle-assisted transdermal drug delivery offers an attractive alternative for local delivery of NSAIDs. However, traditional microneedle arrays fabricated on flat surfaces are unable to deliver drugs effectively across the undulating skin surface of affected finger(s)...
January 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28071596/3d-bioprinting-of-gelma-scaffolds-triggers-mineral-deposition-by-primary-human-osteoblasts
#19
Christine McBeth, Jasmin Lauer, Michael Ottersbach, Jennifer Campbell, Andre Sharon, Alexis F Sauer-Budge
Due to its relatively low level of antigenicity and high durability, titanium has successfully been used as the major material for biological implants. However, because the typical interface between titanium and tissue precludes adequate transmission of load into the surrounding bone, over time, load-bearing implants tend to loosen and revision surgeries are required. Osseointegration of titanium implants requires presentation of both biological and mechanical cues that promote attachment of and trigger mineral deposition by osteoblasts...
January 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28052048/a-biomimetic-mesh-for-treating-female-stress-urinary-incontinence
#20
Kuilin Lai, Jiazhen Zhang, Guoshuai Wang, Xin Luo, Man Liu, Xinzhi Zhang, Yuyu Yuan, Bin Liu, Tao Xu
For patients with medium to severe incontinence, sub-urethral support surgery has a high cure rate, but using synthetic meshes leads to some complications such as mesh erosion/exposure and thigh pain. Autologous or acellular extracellular matrix grafts present few complications but have a high recurrence rate. Regensling(™) is a new sling product made of a synthetic material with a biomimetic structure, aiming to provide long-term mechanical support with a lower complication rate. To assess the safety and effectiveness of Regensling(™), both in vitro and in vivo experiments were performed...
January 4, 2017: Biofabrication
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