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Tilman Ahlfeld, Falko Doberenz, David Kilian, Corina Vater, Paula Korn, Günter Lauer, Anja Lode, Michael Gelinsky
Due to their characteristic resemblance of the mineral component of bone, calcium phosphates are widely accepted as optimal bone substitute materials. Recent research focused on the development of pasty calcium phosphate cement (CPC) formulations, which can be fabricated into various shapes by low-temperature extrusion based additive manufacturing, namely 3D plotting. While it could be demonstrated that sensitive substances like growth factors can be integrated in such printed CPC scaffolds without impairment of their biological activity live cells cannot be suspended in CPC as they may not be functional when enclosed in a solid and stiff matrix...
July 13, 2018: Biofabrication
Hyeonjun Hong, Man-Il Huh, Sang Min Park, Kyoung-Pil Lee, Hong Kyun Kim, Dong Sung Kim
Recently, a compressed collagen has attracted much attention as a potential alternative for a limbal epithelial stem cell (LESC) carrier to treat limbal stem cell deficiency (LSCD), in that it can provide mechanically improved collagen fibrillar structure as compared to a conventional collagen hydrogel. However, its clinical efficacy as an LESC carrier has not yet been studied through in vivo transplantation due to limited mechanical strength to withstand a force induced by surgical suturing and low resistance to enzymatic degradation...
July 6, 2018: Biofabrication
Yang Li, Xulin Jiang, Ling Li, Zhi-Nan Chen, Ge Gao, Rui Yao, Wei Sun
Human induced pluripotent stem cells (hiPSCs) are more likely to successfully avoid the immunological rejection and ethical problems that are often encountered by human embryonic stem cells in various stem cell studies and applications. To transfer hiPSCs from the laboratory to clinical applications, researchers must obtain sufficient cell numbers. In this study, 3D cell printing was used as a novel method for iPSC scalable expansion. Hydroxypropyl chitin (HPCH), utilized as a new type of bioink, and a set of optimized printing parameters were shown to achieve high cell survival (> 90%) after the printing process and high proliferation efficiency (~ 32...
June 28, 2018: Biofabrication
Teng Gao, Gregory James Gillispie, Joshua S Copus, Anil Kumar Pallickaveedu Rajan Asari, Young-Joon Seol, Anthony Atala, James J Yoo, Sang Jin Jin Lee
Three-dimensional (3D) bioprinting has emerged as a promising technique in tissue engineering applications through the precise deposition of cells and biomaterials in a layer-by-layer fashion. However, the limited availability of hydrogel bioinks is frequently cited as a major issue for the advancement of cell-based extrusion bioprinting technologies. It is well known that highly viscous materials maintain their structure better, but also have decreased cell viability due to the higher forces which are required for extrusion...
June 20, 2018: Biofabrication
Liqun Ning, Haoying Sun, Tiphanie Lelong, Romain Guilloteau, Ning Zhu, David J Schreyer, Daniel Xiongbiao Chen
Three-dimensional (3D) bioprinting of biomaterials shows great potential for producing cell-encapsulated scaffolds to repair nerves after injury or disease. For this, preparation of biomaterials and bioprinting itself are critical to create scaffolds with both biological and mechanical properties appropriate for nerve regeneration, yet remain unachievable. This paper presents our study on bioprinting Schwann cell-encapsulated scaffolds using composite hydrogels of alginate, fibrin, hyaluronic acid, and/or RGD peptide, for nerve tissue engineering applications...
June 18, 2018: Biofabrication
Lokesh Karthik Narayanan, Trevor L Thompson, Rohan A Shirwaiker, Binil Starly
Biofabrication processes can affect biological quality attributes of encapsulated cells within constructs. Currently, assessment of the fabricated constructs is performed offline by subjecting the constructs to destructive assays that require staining and sectioning. This drawback limits the translation of biofabrication processes to industrial practice. In this work, we investigate the dielectric response of viable cells encapsulated in bioprinted 3D hydrogel constructs to an applied alternating electric field as a label-free non-destructive monitoring approach...
June 14, 2018: Biofabrication
Stephen W Sawyer, Shivkumar Vishnempet Shridhar, Kairui Zhang, Lucas D Albrecht, Alex B Filip, Jason A Horton, Pranav Soman
Despite the promise of stem cell engineering and the new advances in bioprinting technologies, one of the major challenges in the manufacturing of large scale bone tissue scaffolds is the inability to perfuse nutrients throughout thick constructs. Here, we report a scalable method to create thick, perfusable bone constructs using a combination of cell-laden hydrogels and a 3D printed sacrificial polymer. Osteoblast-like Saos-2 cells were encapsulated within a gelatin methacrylate (GelMA) hydrogel and 3D printed polyvinyl alcohol pipes were used to create perfusable channels...
June 28, 2018: Biofabrication
Jennifer Gansau, Lara Kelly, Conor Timothy Buckley
Cell delivery and leakage during injection remains a challenge for cell-based intervertebral disc regeneration strategies. Cellular microencapsulation may offer a promising approach to overcome these limitations by providing a protective niche during intradiscal injection. Electrohydrodynamic spraying (EHDS) is a versatile one-step approach for microencapsulation of cells using a high voltage electric field. The primary objective of this work was to characterise key processing parameters such as applied voltage (0, 5, 10 or 15 kV), emitter needle gauge (21, 26 or 30 G), alginate concentration (1%, 2% or 3%) and flow rate (50, 100, 250 or 500 μl min-1 ) to regulate the size and morphology of alginate microcapsules as well as subsequent cell viability when altering these parameters...
June 25, 2018: Biofabrication
M Yan, P L Lewis, R N Shah
3D-printing has expanded our ability to produce reproducible and more complex scaffold architectures for tissue engineering applications. In order to enhance the biological response within these 3D-printed scaffolds incorporating nanostructural features and/or specific biological signaling may be an effective means to optimize tissue regeneration. Peptides amphiphiles (PAs) are a versatile supramolecular biomaterial with tailorable nanostructural and biochemical features. PAs are widely used in tissue engineering applications such as angiogenesis, neurogenesis, and bone regeneration...
June 18, 2018: Biofabrication
Vladislav A Parfenov, Elizaveta V Koudan, Elena A Bulanova, Pavel A Karalkin, Frederico DAS Pereira, Nikita E Norkin, Alisa D Knyazeva, Anna A Gryadunova, Oleg F Petrov, Mikhail M Vasiliev, Maxim I Myasnikov, Valery P Chernikov, Vladimir A Kasyanov, Artem Yu Marchenkov, Kenn Brakke, Yusef D Khesuani, Utkan Demirci, Vladimir A Mironov
Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape and capable to fusion have been biofabricated from primary sheep chondrocytes using non-adhesive technology...
June 18, 2018: Biofabrication
Johan Göhl, Kajsa Markstedt, Andreas Mark, Karl Håkansson, Paul Gatenholm, Fredrik Edelvik
3D bioprinting with cell containing bioinks show great promise in the biofabrication of patient specific tissue constructs. To fulfil the multiple requirements of a bioink, a wide range of materials and bioink composition are being developed and evaluated with regard to cell viability, mechanical performance and printability. It is essential that the printability and printing fidelity is not neglected since failure in printing the targeted architecture may be catastrophic for the survival of the cells and consequently the function of the printed tissue...
June 18, 2018: Biofabrication
Chen Chen, Paulus G M Jochems, Lucia Salz, Kerstin Schneeberger, Louis C Penning, Stan F J van de Graaf, Ulrich Beuers, Hans Clevers, Niels Geijsen, Rosalinde Masereeuw, Bart Spee
Investigation of diseases of the bile duct system and identification of potential therapeutic targets are hampered by the lack of tractable in vitro systems to model cholangiocyte biology. Here, we show a step-wise method for the differentiation of murine Lgr5+ liver stem cells (organoids) into cholangiocyte-like cells (CLCs) using a combination of growth factors and extracellular matrix components. Organoid-derived CLCs display key properties of primary cholangiocytes, such as expressing cholangiocyte markers, forming primary cilia, transporting small molecules and responding to farnesoid X receptor agonist...
June 12, 2018: Biofabrication
Lise De Moor, Idriz Merovci, Sarah Baetens, Julien Verstraeten, Paulina Kowalska, Dmitri V Krysko, Winnok H De Vos, Heidi Declercq
Overcoming the problem of vascularization remains the main challenge in the field of tissue engineering. As three-dimensional (3D) bioprinting is the rising technique for the fabrication of large tissue constructs, small prevascularized building blocks were generated that can be incorporated throughout a printed construct, answering the need for a microvasculature within the small micron range (<10 μm). Uniform spheroids with an ideal geometry and diameter for bioprinting were formed, using a high-throughput non-adhesive agarose microwell system...
June 12, 2018: Biofabrication
Donggu Kang, Geunseon Ahn, Donghwan Kim, Hyun-Wook Kang, Seokhwan Yun, Won-Soo Yun, Jin-Hyung Shim, Songwan Jin
Recent advances in three-dimensional bioprinting technology have led to various attempts in fabricating human tissue-like structures. However, current bioprinting technologies have limitations for creating native tissue-like structures. To resolve these issues, we developed a new pre-set extrusion bioprinting technique that can create heterogeneous, multicellular, and multimaterial structures simultaneously. The key to this ability lies in the use of a precursor cartridge that can stably preserve a multimaterial with a pre-defined configuration that can be simply embedded in a syringe-based printer head...
June 6, 2018: Biofabrication
Zhichang Du, Shengli Mi, Xiaoman Yi, Yuanyuan Xu, Wei Sun
Tumour invasion into the surrounding stroma is a critical step in metastasis, and it is necessary to clarify the role of microenvironmental factors in tumour invasion. We present a microfluidic system that simulated and controlled multi-factors of the tumour microenvironment for three-dimensional (3D) assessment of tumour invasion into the stroma. The simultaneous, precise and continuous arrangement of two 3D matrices was visualised to observe the migration of cancer cell populations or single cells by transfecting cells with a fluorescent protein...
June 6, 2018: Biofabrication
Khoon S Lim, Riccardo Levato, Pedro F Costa, Miguel D Castilho, Cesar R Alcala-Orozco, Kim M A van Dorenmalen, Ferry P W Melchels, Debby Gawlitta, Gary J Hooper, Jos Malda, Tim B F Woodfield
Lithography-based three-dimensional (3D) printing technologies allow high spatial resolution that exceeds that of typical extrusion-based bioprinting approaches, allowing to better mimic the complex architecture of biological tissues. Additionally, lithographic printing via digital light processing (DLP) enables fabrication of free-form lattice and patterned structures which cannot be easily produced with other 3D printing approaches. While significant progress has been dedicated to the development of cell-laden bioinks for extrusion-based bioprinting, less attention has been directed towards the development of cyto-compatible bio-resins and their application in lithography-based biofabrication, limiting the advancement of this promising technology...
May 11, 2018: Biofabrication
Shida Miao, Haitao Cui, Margaret Nowicki, Se-Jun Lee, José Almeida, Xuan Zhou, Wei Zhu, Xiaoliang Yao, Fahed Masood, Michael W Plesniak, Muhammad Mohiuddin, Lijie Grace Zhang
4D printing is a highly innovative additive manufacturing process for fabricating smart structures with the ability to transform over time. Significantly different from regular 4D printing techniques, this study focuses on creating novel 4D hierarchical micropatterns using a unique photolithographic-stereolithographic-tandem strategy (PSTS) with smart soybean oil epoxidized acrylate (SOEA) inks for effectively regulating human bone marrow mesenchymal stem cell (hMSC) cardiomyogenic behaviors. The 4D effect refers to autonomous conversion of the surficial-patterned scaffold into a predesigned construct through an external stimulus delivered immediately after printing...
May 2, 2018: Biofabrication
F Kawecki, W P Clafshenkel, F A Auger, J-M Bourget, J Fradette, R Devillard
A major challenge during the engineering of voluminous bone tissues is to maintain cell viability in the central regions of the construct. In vitro prevascularization of bone substitutes relying on endothelial cell bioprinting has the potential to resolve this issue and to replicate the native bone microvasculature. Laser-assisted bioprinting (LAB) commonly uses biological layers of hydrogel, called 'biopapers', to support patterns of printed cells and constitute the basic units of the construct. The self-assembly approach of tissue engineering allows the production of biomimetic cell-derived bone extracellular matrix including living cells...
April 30, 2018: Biofabrication
Xinda Li, Libiao Liu, Xinzhi Zhang, Tao Xu
Artificial blood vessels must be strong, flexible, and must not lead to blockage after implantation. It is therefore important to select an appropriate fabrication process for products to meet these requirements. This review discusses the current methods for making artificial blood vessels, focusing on fabrication principle, materials, and applications. Among these methods, 3D printing is very promising since it has the unique capability to make complicated three-dimensional structures with multiple types of materials, and can be completely digitalized...
April 30, 2018: Biofabrication
Lothar Koch, Andrea Deiwick, Annika Franke, Kristin Schwanke, Axel Haverich, Robert Zweigerdt, Boris Chichkov
Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to the patient without immunological concerns. 3D printing of hiPSCs could enable the generation of functional organs for replacement therapies or realization of organ-on-chip systems for individualized medicine. Printing of living cells was demonstrated with immortalized cell lines, primary cells, and adult stem cells with different printing technologies and biomaterials...
April 25, 2018: Biofabrication
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