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Iranian Journal of Microbiology

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https://www.readbyqxmd.com/read/29487737/recent-approaches-in-whole-cell-pneumococcal-vaccine-development-a-review-study
#1
REVIEW
Mona Mohammadzadeh, Setareh Mamishi, Babak Pourakbari, Shima Mahmoudi
Despite the availability of relatively effective vaccines, Streptococcus pneumoniae still causes widespread morbidity and mortality. Current vaccines contain free polysaccharides or protein-polysaccharide conjugates, but do not induce protection against serotypes that are not included in the vaccines. Therefore, developing alternative vaccines is of high priority and importance. Several investigators have identified protective antigens common to pneumococci of many or all serotypes. Malley et al. in their study, have recommended unencapsulated whole cells, as an alternative vaccine, a number of such antigens unoccluded by capsule were presented in a native configuration in 2001...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487736/assessment-and-comparison-of-the-pathogenicity-of-sheeppox-virus-strains-isolated-in-morocco
#2
Saida Hajjou, Khadija Khataby, Souad Amghar, Mustapha El Fahime, Mehdi El Harrak, Malika Fakiri, Chafiqa Loutfi
Background and Objectives: Sheeppox virus causes systemic disease in sheep that is often associated with high morbidity and mortality. Protection against sheep pox is mainly based on medical prophylaxis, vaccination being the only way. In Morocco, and up to now, there is no available information about local challenge strain to use for controlling the efficiency of vaccines produced against sheep pox. Hence, the objective of the present study was to evaluate and compare the pathogenicity of seven Sheeppox virus (SPVs) isolates from 1993-1995 in Morocco...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487735/antifungal-susceptibility-patterns-of-colonized-candida-species-isolates-from-immunocompromised-pediatric-patients-in-five-university-hospitals
#3
Parisa Badiee, Maral Choopanizadeh, Abdolkarim Ghadimi Moghadam, Ali Hossaini Nasab, Hadis Jafarian, Ahmad Shamsizadeh, Jafar Soltani
Background and Objectives: Colonization of Candida species is common in pediatric patients admitted to hematology-oncology wards. The aim of this study was to identify colonized Candida species and their susceptibility patterns in hematologic pediatric patients. Materials and Methods: Samples were collected from mouth, nose, urine and stool of the patients admitted to five university hospitals and cultured on sabouraud dextrose agar. The isolates were identified by API 20 C AUX system and their susceptibility patterns were evaluated by CLSI M27-A3 and S4...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487734/natural-overproduction-of-catalase-by-kocuria-sp-asb-107-extraction-and-semi-purification
#4
Maryam Najari, Zahra Moosavi-Nejad, Elham Sadat Seyad Javad Javaheri, Ezat Asgarani
Background and Objectives: Because of importance of catalase in various industries, efforts have been made to find more suitable bacterial sources for catalase production. Kocuria is one of well-known catalase-producing genus. This is the first report about a new catalase-overproducing bacterial strain, Kocuria sp. ASB 107. Materials and Methods: Kocuria sp. ASB 107 had been isolated from Abe-Siah Spring in Ramsar in our previous report. The bacterial biomass freezed, thawed and then lysed by three different operations separately: ultrasound, lysing buffer and enzymatic digestion...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487733/antagonistic-activities-of-some-probiotic-lactobacilli-culture-supernatant-on-serratia-marcescens-swarming-motility-and-antibiotic-resistance
#5
Roya Vahedi-Shahandashti, Rouha Kasra-Kermanshahi, Maliheh Shokouhfard, Parinaz Ghadam, Mohammad Mehdi Feizabadi, Shahram Teimourian
Background and Objectives: Serratia marcescens , a potentially pathogenic bacterium, benefits from its swarming motility and resistance to antibiotic as two important virulence factors. Inappropriate use of antibiotics often results in drug resistance phenomenon in bacterial population. Use of probiotic bacteria has been recommended as partial replacement. In this study, we investigated the effects of some lactobacilli culture supernatant on swarming, motility and antibiotic resistance of S...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487732/isolation-and-identification-of-komagataeibacter-xylinus-from-iranian-traditional-vinegars-and-molecular-analyses
#6
Paria Sadat Lavasani, Elahe Motevaseli, Mahdieh Shirzad, Mohammad Hossein Modarressi
Background and Objectives: Acetic acid bacteria (AAB) are one of the major interests of researchers. Traditional vinegars are suitable sources of AAB because they are not undergone industrial process like filtering and adding preservatives. Komagataeibacter xylinus as a member of AAB is known as the main cellulose producer among other bacteria. The purpose of the current study was to isolate the bacteria from traditional vinegars and its molecular analyses. Materials and Methods: Vinegar samples were collected...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487731/evidence-of-heat-resistant-microorganisms-with-a-special-emphasis-on-filamentous-actinomycetes-in-hyper-arid-soils-of-gandom-beryan-area-lut-desert-iran
#7
Somaye Mazkour, Saeid Hosseinzadeh, Seyed Shahram Shekarforoush
Background and Objectives: In the present study, the Lut Desert, Iran was chosen as one of the hottest places in the world (with the recorded temperature of 70.7°C during 2003-2009) to find out whether any heat-resistant microorganisms were present in the soil. Materials and Methods: The samples were collected from surface and depth of three identified places of Gandom Beryan in the Lut Desert. Chemical analysis and enumeration of the total bacteria, yeasts and molds were performed...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487730/fibrinogen-and-mucin-binding-activity-of-ef0737-a-novel-protein-of-enterococcus-faecalis
#8
Samira Sabzi, Rahil Mashhadi, Mohammad Reza Pourmand
Background and Objectives: Enterococcus faecalis is the leading cause of several human infections. This opportunist pathogen expresses surface components that have various functions in the infection process including bacterial adhesion, lytic activity, and induction of host immune responses. EF0737, a novel cell wall associated protein, may play an important role in pathogenesis of E. faecalis , based on our experiments. This study was conducted to clone and express EF0737 and demonstrate its interaction with biotinylated plasma proteins and patients' sera...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487729/prevalence-of-acinetobacter-baumannii-bacteremia-in-intensive-care-units-of-ibn-rochd-university-hospital-casablanca
#9
Assiya El Kettani, Fakhreddine Maaloum, Idrissa Diawara, Khalid Katfy, Nadia Harrar, Khalid Zerouali, Houria Belabbes, Naima Elmdaghri
Background and Objectives: Acinetobacter baumannii bacteremia are grave because of the multi-resistance of the organism to antibiotics. This study aimed to determine the prevalence of A. baumannii isolated from blood cultures and to describe their antibiotic resistance patterns. Materials and Methods: A retrospective longitudinal study was conducted on blood cultures between 2010 and 2014 from all Ibn Rochd University Hospital intensive care units; it was based on the exploitation of microbiology laboratory database (duplicates were excluded)...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29487728/the-high-prevalence-of-mycobacterium-tuberculosis-beijing-strain-at-an-early-age-and-extra-pulmonary-tuberculosis-cases
#10
Hanifeh Erie, Hami Kaboosi, Naeme Javid, Hesamaddin Shirzad-Aski, Masoumeh Taziki, Maya Babaee Kuchaksaraee, Ezzat Allah Ghaemi
Background and Objectives: Tuberculosis (TB) is still responsible for a wide range of deaths worldwide. Beijing genotype is one of the most important and virulent strains in Mycobacterium tuberculosis . This study was designed for determination Beijing genotypes of M. tuberculosis in Golestan province, north of Iran. Materials and Methods: In the current descriptive study, 238 clinical MTB isolates, obtained from patients with pulmonary and extra-pulmonary TB in north of Iran, were evaluated...
December 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29296276/preparation-of-transgenic-iranian-lizard-leishmania-coding-hil-12
#11
Tahereh Donyavi, Mojgan Bandehpour, Bahram Kazemi
Background and Objectives: Leishmania are intracellular flagellate protozoan parasites cause a wide spectrum of clinical manifestations in human. The immunological basis for resistance against leishmaniasis depends on Thl responses in the course of performance of cytokines like IL-12. In this study, a transgenic Leishmania coding human IL-12 was produced that can be used in Leishmanization. Materials and Methods: A fragment of Iranian lizard Leishmania (I.L.L) gene, named Cysteine Peptidase C (CPC), was amplified separately as two parts with PCR reaction...
October 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29296275/consortium-inoculum-of-five-thermo-tolerant-phosphate-solubilizing-actinomycetes-for-multipurpose-biofertilizer-preparation
#12
Arusha P Nandimath, Dilip D Karad, Shantikumar G Gupta, Arun S Kharat
Background and Objectives: Alkaline pH of the soil facilitates the conversion of phosphate present in phosphate fertilizer applied in the field to insoluble phosphate which is not available to plants. Problem of soluble phosphate deficiency arises, primarily due to needless use of phosphate fertilizer. We sought to biofertilizer with the thermo-tolerant phosphate solubilizing actinomycetes consortium that could convert insoluble phosphate to soluble phosphate at wider temperature range...
October 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29296274/the-role-of-zoonotic-chlamydial-agents-in-ruminants-abortion
#13
Sara Barati, Naghmeh Moori-Bakhtiari, Masoud Ghorbanpoor Najafabadi, Hassan Momtaz, Leili Shokuhizadeh
Background and Objectives: Enzootic abortion of ewes (EAE) is caused by infection of sheep and goats by Chlamydia abortus bacterium. Chlamydial abortion in bovine could occur by Chlamydia abortus, Chlamydia psittaci and Chlamydia pecorum. C. psittaci is the causative agent of psittacosis or ornithosis disease in humans and birds. It also causes acute pneumonia in cattle and sheep. The present study aimed at surveying the role of chlamydial agents in ruminants abortion. Materials and Methods: A total of 117 aborted material samples (Cotyledon, liver, spleen, and abomasal contents of fetus) from 9 cattle and 100 sheep in Shahr-e-Kord and 8 sheep from Bagh-e-Malek were collected from different herds with abortion history during the lambing periods from 2014 to 2016...
October 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29296273/new-delhi-metallo-%C3%AE-lactamase-1-producing-klebsiella-pneumoniae-isolates-in-hospitalized-patients-in-kashan-iran
#14
Farzaneh Firoozeh, Zeinab Mahluji, Ehsaneh Shams, Ahmad Khorshidi, Mohammad Zibaei
Background and Objectives: New Delhi metallo-ß-lactamase (NDM) is a newly emerging metallo-ß-lactamases, which can destroy all β-lactams including carbapenems. Therefore, this study aimed at evaluating New Delhi metallo-ß-lactamase-1-production in clinical isolates of Klebsiella pneumoniae in Kashan, Iran. Materials and Methods: In a cross-sectional study, 181 K. pneumoniae isolates were collected from clinical samples of patients, who referred to Shahid Beheshi hospital in Kashan during November 2013 and October 2014...
October 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29296272/a-study-of-prevalence-of-shigella-species-and-antimicrobial-resistance-patterns-in-paediatric-medical-center-ahvaz-iran
#15
Roya Nikfar, Ahmad Shamsizadeh, Marjan Darbor, Soheila Khaghani, Mina Moghaddam
Background and Objectives: Shigella infections are one of the major causes of diarrhea worldwide, and especially in developing countries. Antimicrobial resistance has complicated the empirical treatment. The aim of this study was to define the clinical and antibiotic resistance patterns of Shigella gastroenteritis cases. Materials and Methods: Stool samples of patients with diarrhea and fever diagnosed with shigellosis were collected, from June 2013 to May 2014 at Abuzar Hospital, Iran...
October 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29296271/epidemiologic-analysis-of-central-vein-catheter-infection-in-burn-patients
#16
Maryam Roham, Mahnoush Momeni, Mohsen Saberi, Rahil Kheirkhah, Ali Jafarian, Hossein Rahbar
Background and Objectives: Currently, there are no well-defined guidelines or criteria for catheter-site care in burn patients, and there is little information about the epidemiology of central vein catheter (CVC) infection in such patients. This study aimed at addressing the epidemiological aspect of CVC infection in a sample of Iranian burn patients admitted to the largest referral burn center in Iran, Motahari Burn Center. Materials and Methods: A total of 191 burn patients were eligible for the study...
October 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29296270/phenotypic-and-genotypic-characterization-of-macrolide-resistance-among-staphylococcus-aureus-isolates-in-isfahan-iran
#17
Hossein Sedaghat, Bahram Nasr Esfahani, Sina Mobasherizadeh, Azhar Sallari Jazi, Mehrdad Halaji, Parisa Sadeghi, Mohammad Emaneini, Seyed Asghar Havaei
Background and Objectives: Macrolide, lincosamide and streptogramin B (MLSB ) are noteworthy antibiotics for the treatment of Staphylococcus aureus infections. The purpose of this study, was to determine the phenotypic and genotypic characterization of macrolide resistance, among S. aureus , isolated from clinical samples and nasal swabs. Materials and Methods: Totally, 162 non-duplicate S. aureus isolates were collected from clinical samples and nasal swabs, from patients and healthcare workers (HCWs), between March 2016 and September 2016, at four teaching hospitals in Isfahan...
October 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29296269/clinicomicrobiological-spectrum-of-infective-endocarditis-from-a-tertiary-care-centre-in-south-india
#18
Kanne Padmaja, Sukanya Sudhaharan, Lakshmi Vemu, Oruganti Sai Satish, Padmasri Chavali, Mamidi Neeraja
Background and Objectives: Infective endocarditis (IE) is a microbial infection of the endothelial surface of the cardiac-valves. Rapid diagnosis, effective treatment and prompt recognition of complications are essential, in order to improve the outcome. We retrospectively reviewed and determined the clinical characteristics, microbiological profile and management strategies of IE cases, changing microbial spectrum of pathogens and outcome in Native Valve Endocarditis (NVE) and Prosthetic Valve Endocarditis (PVE) cases...
October 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29238461/cloning-expression-and-purification-of-a-polytopic-antigen-comprising-of-surface-antigens-of-toxoplasma-gondii
#19
Abbas Alibakhshi, Mojgan Bandehpour, Bahram Kazemi
Background and Objectives: Polytopic antigens are recently applied for replacing crude antigens, for control of infectious agents. The surface of the Toxoplasma is covered with immunogenic antigens namely surface antigens (SAGs). These antigens possess several immunogenic epitopes, inducing immune responses. Materials and Methods: In this study, a DNA construct comprising of sequences encoding epitopes from SAG1, 2 and 3 was designed and cloned into pET28a expression vector and subsequently expressed and purified, using Ni-NTA column...
August 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29238460/synthetic-peptides-mimicking-lipopolysaccharide-as-a-potential-vaccine-candidates-against-vibrio-cholerae-serogroup-o1
#20
Fatemeh Mohammad Pour Ghazi, Seyed Latif Mousavi Gargari
Background and Objectives: Cholera is a life-threatening diarrhea caused mainly by Gram-negative marine habitant Vibrio cholerae serogroup O1. Cholera vaccination is limited mainly to developed countries, due to the cumbersome and expensive task of vaccine production. In the present work, the aim was to study the immunogenicity of the synthetic mimotopes through two different routes of injection and oral administration. Lipopolysaccharide (LPS) is one of the immunogenic components in Gram-negative bacteria, which cannot be used as a vaccine candidate, due to its high toxic effect...
August 2017: Iranian Journal of Microbiology
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