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Iranian Journal of Microbiology

Shahram Habibzadeh, Hadi Peeri-Doghaheh, Jafar Mohammad-Shahi, Elham Mobini, Samira Shahbazzadegan
BACKGROUND AND OBJECTIVES: Trans-placental transmission of parvovirus B19 during pregnancy can causes adverse outcomes. Regarding its importance in prenatal care, we decided to study prevalence of parvovirus B19 infection among pregnant woman in Ardabil, Iran. MATERIALS AND METHODS: In a community based study with a cluster sampling, 350 pregnant women that attended in health care centers in Ardabil were selected. Serum samples were collected and Anti-B19 specific IgG was detected using commercial enzyme-linked immunosorbent assays (Euroimmune Elisa kit, Germany)...
June 2016: Iranian Journal of Microbiology
Mostafa Dahmardehei, Faranak Alinejad, Fereshteh Ansari, Mahnaz Bahramian, Mitra Barati
BACKGROUND AND OBJECTIVES: Infection is the most common cause of death among burnt patients and infection control decrease the rate of mortality. The use of sticky mat can control contamination by preventing the entrance microorganisms into the hospital wards. This study was designed to evaluate the sticky mats effect in reduction of microorganism's entry by personnel shoes to burn intensive care unit (BICU). MATERIALS AND METHODS: This is a simple cross sectional study...
June 2016: Iranian Journal of Microbiology
Ramezan Ali Ataee, Ali Mehrabi-Tavana, Seyed Mohammad Javad Hosseini, Farshad Kaviani
BACKGROUND AND OBJECTIVES: Neisseria meningitidis is transmitted from person-to-person. Thus, close contact with a healthy carrier can facilitate the spread of the bacteria and lead to life-threatening meningococcal disease. The aim of this study was to identify oropharyngeal carriers of N. meningitidis in volunteers preparing for military service before vaccination. MATERIALS AND METHODS: In a cross-sectional study, 226 volunteers entering military service were referred to the Shemiranat Health Center for meningococcal vaccination and assayed...
June 2016: Iranian Journal of Microbiology
Abbas Abdollahi, Shahla Mansouri, Jafar Amani, Mahdi Fasihi-Ramandi, Mohammad Moradi
BACKGROUND AND OBJECTIVES: Brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. Because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against Brucella is desirable. In this study, we evaluated the immune responses induced by a designed recombinant chimera protein in murine model. MATERIALS AND METHODS: Three immunodominant antigens of Brucella have been characterized as potential immunogenic and protective antigens including: trigger factor (TF), Omp31 and Bp26 were fused together by EAAAK linkers to produce a chimera (structure were designed in silico), which was synthesized, cloned, and expressed in E...
June 2016: Iranian Journal of Microbiology
Taifeh Hoseinzadeh, Reza Ghanbarpour, Farokh Rokhbakhsh-Zamin
BACKGROUND AND OBJECTIVES: Diarrheagenic Escherichia coli (DEC) strains are a major cause of intestinal syndromes in the developing countries. The aim of this study was to determine the prevalence of enterotoxigenic E. coli (ETEC) and enteroinvasive E. coli (EIEC) in relation to phylogenetic background from patients with diarrhea. MATERIALS AND METHODS: A total of 110 E. coli isolates were obtained from diarrhea patients in Sirjan, southeast of Iran. The E. coli isolates were confirmed using biochemical and bacteriological tests...
June 2016: Iranian Journal of Microbiology
Padmaja Ananth-Shenoy, Shashidhar Vishwanath, Ryumzook Targain, Seema Shetty, Gabriel Sunil-Rodrigues, Chiranjay Mukhopadhyay, Kiran Chawla
BACKGROUND AND OBJECTIVES: Anaerobic bacteria are recognized as important pathogens in surgical infections. However, they are the most overlooked microorganisms by the clinic and the laboratory because of the tedious culture techniques with longer turn-around times. The study was aimed to analyze the frequency of anaerobic bacterial surgical infections and their predisposing factors. MATERIALS AND METHODS: A retrospective study was conducted over a period of two years including patients with surgical infections...
June 2016: Iranian Journal of Microbiology
Mansour Rezaei, Alisha Akya, Azam Elahi, Keyghobad Ghadiri, Somayeh Jafari
BACKGROUND AND OBJECTIVES: Citrobacter freundii is an opportunistic pathogen causing nosocomial infections and resistant to various antibiotics. We aimed to determine the clonal relationship of C. freundii isolates using Pulsed-Field Gel Electrophoresis (PFGE). MATERIALS AND METHODS: Fifty clinical isolates of C. freundii were collected from the main hospital in Kermanshah. After antibiotic susceptibility testing and screening for extended-spectrum beta lactamase (ESBL), all isolates were genotyped by PFGE...
June 2016: Iranian Journal of Microbiology
Amir Peymani, Taghi Naserpour-Farivar, Moein Yeylagh-Beygi, Shahiin Bolori
BACKGROUND AND OBJECTIVES: The emergence of plasmid-mediated AmpC (pAmpC) β-lactamases conferring resistance to third-generation cephalosporins has become a major clinical concern worldwide. The aims of this study were to determine the prevalence of pAmpC-producing E. cloacae isolates and typing of them in Qazvin and Tehran provinces, Iran. MATERIALS AND METHODS: A total of 120 cefoxitin non-susceptible isolates of E. cloacae were obtained from educational hospitals of Qazvin and Tehran, Iran...
June 2016: Iranian Journal of Microbiology
Sareh Sadat Hosseini, Mohammad Niakan, Horieh Saderi, Mitra Motallebi, Morovat Taherikalani, Khairollah Asadollahi, Mohammad Emaneini
BACKGROUND AND OBJECTIVES: Macrolide, lincosamide and streptogramin type B (MLSB) antibiotics are important in the treatment of Staphylococcus aureus infections and existence of isolates with ability to resist against MLSB antibiotics is worrisome. MATERIALS AND METHODS: In this cross sectional study, 101 S. aureus isolates were collected from patients of five selected hospitals in Tehran over a period of five months. Disk diffusion tests and differentiation between constitutive and inducible resistances were carried out by D-test...
June 2016: Iranian Journal of Microbiology
Willem B van Leeuwen
No abstract text is available yet for this article.
June 2016: Iranian Journal of Microbiology
Hadis Kiani, Manoochehr Makvandi, Alireza Samarbafzadeh, Ali Teimoori, Niloofar Nisi, Hamide Mehravaran, Hashem Radmehr, Zeinab Hosseini, Azadeh Haghi, Toran Shahani, Mehran Varnaseri, Nastran Ranjbari
BACKGROUND AND OBJECTIVES: Human Herpes 6 virus (HHV-6) could remain latent and chronic in the host cells after primary infection. HHV-6 genome encodes certain transactivation proteins which may results in development of malignant lymphoma. The association of human herpes six virus (HHV-6) infection and Hodgkin and Non-Hodgkin lymphomas is strongly supported by epidemiological studies. The aim of this study was to determine the prevalence of HHV-6 among the patients with Hodgkin, Non-Hodgkin's lymphoma...
April 2016: Iranian Journal of Microbiology
Parviz Majidy, Mazaher Khodabandehloo, Nammam-Ali Azadi
BACKGROUND AND OBJECTIVES: Varicella Zoster Virus (VZV) infection in pregnant women can cause complications for the mother and fetus. The aim of this study was to assess the immunity against VZV among young women before marriage. MATERIALS AND METHODS: In this cross-sectional study 250 women attending health centers in Sanandaj, Iran, for pre-marital medical check-up were randomly selected. The VZV IgG measured by ELISA and demographic characteristics of participants including their age, place of residence, number of siblings, occupation, education and history of chickenpox were also recorded...
April 2016: Iranian Journal of Microbiology
Farzan Modarresi, Omid Azizi, Mohammad Reza Shakibaie, Mohammad Motamedifar, Shahla Mansouri
BACKGROUND AND OBJECTIVES: In present study we aimed to clone the luxI gene encoding N-acyl-homoserine synthase detected in clinical isolates of Acinetobacter baumannii and study its expression in Escherichia coli transformants. MATERIALS AND METHODS: Four A. baumannii hospital strains which demonstrated strong biofilm activity were selected in this investigation. The presence of luxI gene was detected using PCR technique. Purified PCR product DNA was initially cloned into pTG19 and transformed to E...
April 2016: Iranian Journal of Microbiology
Roghayeh Teimourpour, Hosna Zare, Ramazan Rajabnia, Yousef Yahyapour, Zahra Meshkat
BACKGROUND AND OBJECTIVES: HBHA and Mtb32C have been isolated from culture supernatants of Mycobacterium tuberculosis (M. tuberculosis) and Mycobacterium bovis (M. bovis) and their immunogenicity previously studies have been confirmed. In this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (Mtb32C-HBHA), in producing new chimeric protein under the in-vitro condition was examined. MATERIALS AND METHODS: In present study Huh7...
April 2016: Iranian Journal of Microbiology
Fatemeh Yeganeh-Sefidan, Reza Ghotaslou, Mohammad Taghi Akhi, Mohammad Reza Sadeghi, Yalda Mohammadzadeh-Asl, Hussein Bannazadeh Baghi
BACKGROUND AND OBJECTIVES: The emergence and spread of multidrug resistant (MDR) and extended spectrum β-lactamase (ESBL) producing strains reduces the number of effective drugs that can be used for treatment. The aim of this study was to evaluate the susceptibility profile of Enterobacteriaceae isolated from UTIs, specifically MDR and ESBL producing strains, to fosfomycin and other antibiotics. MATERIALS AND METHODS: The study was performed during a 6 month period (February 2014 to August 2015)...
April 2016: Iranian Journal of Microbiology
Sukanya Sudhaharan, Padmasri Chavali, Lakshmi Vemu
BACKGROUND AND OBJECTIVES: Brain abscess remains a potentially fatal central nervous system (CNS) disease, especially in developing countries. Anaerobic abscess is difficult to diagnose because of cumbersome procedures associated with the isolation of anaerobes. MATERIALS AND METHODS: This is a hospital-based retrospective microbiological analysis of 430 brain abscess materials (purulent aspirates and/or tissue), for anaerobic organisms, that were received between 1987-2014, by the Microbiology Laboratory in our Institute...
April 2016: Iranian Journal of Microbiology
Farhan Zameer, Rukmangada Ms, Jyoti Bala Chauhan, Shaukath Ara Khanum, Pramod Kumar, Aishwarya Tripurasundari Devi, Nagendra Prasad Mn, Dhananjaya Bl
BACKGROUND AND OBJECTIVES: Adhesion and colonization are prerequisites for the establishment of bacterial pathogenesis. The biofilm development of Pseudomonas aeruginosa was assessed on adhesive surfaces like dialysis membrane, stainless steel, glass and polystyrene. MATERIALS AND METHODS: Microtiter plate biofilm assay was performed to assess the effect of nutrient medium and growth parameters of P. aeruginosa. Further, its growth on adhesive surfaces namely hydrophilic (dialysis membrane) and hydrophobic (polystyrene plate, square glass and stainless steel coupon) was assessed...
April 2016: Iranian Journal of Microbiology
Sahar Irankhah, Mohammad Reza Soudi, Sara Gharavi
BACKGROUND AND OBJECTIVES: The US Environmental Protection Agency has suggested faecal enterococci as the primary bacterial indicators. Of more importance is their direct correlation with swimmer-associated gastroenteritis in recreation water quality monitoring. In contrast to other seawater bodies with 3.5% salinity, the recreational waters in the southern coast of the Caspian Sea possess its own salinity (about 1% w/v) and thus require further investigations to determine the capacity of Enterococcus faecalis as the sole primary microbial index in this unique aquatic environment...
April 2016: Iranian Journal of Microbiology
Azam Aliasghari, Mohammad Rabbani Khorasgani, Sedigheh Vaezifar, Fateh Rahimi, Habibollah Younesi, Maryam Khoroushi
BACKGROUND AND OBJECTIVES: The most prevalent and worldwide oral disease is dental caries that affects a significant proportion of the world population. There are some classical approaches for control, prevention and treatment of this pathologic condition; however, the results are still not completely successful. Therefore new methods are needed for better management of this important challenge. Chitosan is a natural and non-toxic polysaccharide with many biological applications, particularly as an antimicrobial agent...
April 2016: Iranian Journal of Microbiology
Elnaz Bayroodi, Razieh Jalal
BACKGROUND AND OBJECTIVES: Bacterial resistance to conventional antibiotics has become a widespread public health problem. The aim of this study was to investigate the influence of zinc oxide nanoparticles (ZnO NPs) on the antibacterial activity of several conventional antibiotics against Pseudomonas aeruginosa. MATERIALS AND METHODS: ZnO NPs were prepared by solvothermal method and dispersed in glycerol with the help of ammonium citrate as a dispersant. The antibacterial effects of the resulting ZnO nanofluid, ceftazidime, tobramycin, and ciprofloxacin were investigated against two P...
April 2016: Iranian Journal of Microbiology
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