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Iranian Journal of Microbiology

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https://www.readbyqxmd.com/read/28491253/isolation-molecular-identification-and-antifungal-susceptibility-profiles-of-vaginal-isolates-of-candida-species
#1
Ali Rezaei-Matehkolaei, Shokoofe Shafiei, Ali Zarei-Mahmoudabadi
BACKGROUND AND OBJECTIVES: Vulvovaginal candidiasis is a common fungal infection among women during reproductive ages. Although, Candida albicans is accounted as the main etiologic agent of vaginitis, non-albicans species have arisen during last years. Resistant to antifungal drugs especially, fluconazole has been more reported by researchers from around the World. The aims of this study were to determine the prevalence of vulvovaginal candidiasis among suspected patients with vaginitis, the frequency of Candida species, and the susceptibility profiles of isolates to caspofungin, fluconazole and clotrimazole...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491252/inhibitory-effects-of-carvacrol-on-the-expression-of-secreted-aspartyl-proteinases-1-3-in-fluconazole-resistant-candida-albicans-isolates
#2
Seyedeh Sedigheh Hosseini, Mohammad Hossein Yadegari, Masoumeh Rajabibazl, Ezzat Allah Ghaemi
BACKGROUND AND OBJECTIVES: Secreted Aspartyl Proteinase (SAP) is one of the main virulence factors in the pathogenesis of Candida. This enzyme is encoded by a family of at least ten genes. Among these genes, the role of SAP1-3 in mucosal infections is evident. This study aimed to investigate the expression of SAP1-3 genes of Candida albicans isolates after treatment with Echinophora platyloba extract, carvacrol and caspofungin drug. MATERIALS AND METHODS: Vaginal samples of 68 women with suspected vaginitis were obtained and cultured...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491251/significance-of-igg-optical-density-ratios-index-value-in-single-reactive-anti-dengue-virus-igg-capture-elisa
#3
Shiv Sekhar Chatterjee, Ankush Sharma, Shilpee Choudhury, Sushil Kumar Chumber, Mandeep Kaur, Ras Bage, Nittin Parkhe, Uma Khanduri
BACKGROUND AND OBJECTIVES: A single reactive IgG anti-Dengue virus ELISA test in the absence of IgM antibodies or NS1 antigen may denote current infection or past exposure to the virus. To determine whether IgG index value can be used to identify true current dengue infection we conducted a prospective observational study. MATERIALS AND METHODS: Suspected dengue patients (n =1745) were tested in their first specimen by MAC-ELISA, GAC-ELISA and NS1 antigen ELISA...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491250/prevalence-of-hepatitis-c-virus-genotype-3a-in-patients-with-hodgkin-and-non-hodgkin-lymphoma
#4
Hashem Radmehr, Manoochehr Makvandi, Alireza Samarbafzadeh, Ali Teimoori, Niloofar Neisi, Mojtaba Rasti, Sara Abasifar, Hasan Soltani, Samaneh Abbasi, Hadis Kiani, Hamide Mehravaran, Azarakhsh Azaran, Toran Shahani
BACKGROUND AND OBJECTIVES: Hepatitis C virus (HCV) is a major public health problem worldwide. Replication and persistence of HCV genome have been described in the liver tissue as well as B cells lymphocyte. Several investigations have reported that long-term persistence of HCV in B cells may result in Hodgkin and Non-Hodgkin lymphoma. This study was aimed to determine frequency of HCV RNA in histological tissues obtained from patients suffered from Hodgkin and Non-Hodgkin lymphoma. MATERIALS AND METHODS: 52 formalin-fixed paraffin-embedded tissue blocks including 23 (44...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491249/the-seroprevalence-of-mycoplasma-pneumoniae-igm-and-igg-antibodies-in-patients-with-ischemic-stroke
#5
Maryam Roham, Khatereh Anbari, Samira Mirhabibi, Gholamreza Goudarzi
BACKGROUND AND OBJECTIVES: Association between Mycoplasma pneumoniae infection and increased risk for brain stroke has been well understood. Hence, the value of serologic tests for assessing causative relationship between this infection and brain stroke seems to be high. The present study aimed to determine serum level of anti-Mycoplasma pneumoniae antibodies in patients with brain stroke and to compare it with non-stroke patients. MATERIALS AND METHODS: This cross-sectional study was performed on 97 consecutive ischemic stroke patients and 97 sex and age-matched non-stroke patients...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491248/the-comparison-of-five-low-cost-liquid-formulations-to-preserve-two-phosphate-solubilizing-bacteria-from-the-genera-pseudomonas-and-pantoea
#6
Sanaz Goljanian-Tabrizi, Soleiman Amiri, Donya Nikaein, Zeinab Motesharrei
BACKGROUND AND OBJECTIVES: Phosphorus is one of the low bioavailable macroelements. Use of microorganisms in biofertilizers could release phosphorus from insoluble compounds. Pseudomonas putida P13 and Pantoea agglomerans P5 are well recognized for application as phosphate solubilizing bioinoculants and are used as solid carrier based. Liquid bioinoculants are preferred for economizing production process and longer shelf-life. MATERIALS AND METHODS: Five low cost liquid formulations were examined...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491247/the-antibacterial-activity-of-methanolic-anacyclus-pyrethrum-and-pistacia-lentiscus-l-extract-on-escherichia-coli
#7
Noushin Jalayer-Naderi, Mohammad Niakan, Elham Khodadadi, Maryam Mohamadi-Motlagh
BACKGROUND AND OBJECTIVES: Antibiotic therapy is the main choice in treatment of Escherichia coli induced infections. Using herbal medication is an alternative choice in treatment of diseases. The aim of this study was to determine the antibacterial activity of two traditionally used herbs in Iranian medicine, Anacyclus pyrethrum and Pistacia lentiscus L., on Escherichia coli. MATERIALS AND METHODS: The antibacterial effect of methanolic extract of Anacyclus pyrethrum and Pistacia lentiscus L...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491246/significance-of-coagulase-negative-staphylococcus-from-blood-cultures-persisting-problems-and-partial-progress-in-resource-constrained-settings
#8
Shailpreet K Sidhu, Sita Malhotra, Pushpa Devi, Arpandeep K Tuli
BACKGROUND AND OBJECTIVES: Coagulase negative Staphylococcus (CoNS) is frequently isolated from blood cultures but their significance is difficult to interpret. CoNS bacteria which are often previously dismissed as culture contaminants are attracting greater importance as true pathogens in the past decades. Clinical evaluation of these isolates suggests that although there is a relative increase of CoNS associated bloodstream infections in recent years, the microorganisms still remain the most common contaminants in blood cultures...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491245/frequency-evaluation-of-genes-encoding-siderophores-and-the-effects-of-different-concentrations-of-fe-ions-on-growth-rate-of-uropathogenic-escherichia-coli
#9
Behnoush Khasheii, Shaghayegh Anvari, Ailar Jamalli
BACKGROUND AND OBJECTIVES: Bacteria need iron for growth and most of them can actively acquire Fe ions using especial iron-chelating proteins which named siderophores. We aimed to determine the frequencies of iucA, iroN and irp2 genes in the uropathogenic Escherichia coli (UPEC) isolates. We also analyzed the effects of siderophore genes beside iron supplements on growth rate of the isolates. MATERIALS AND METHODS: Totally, 170 E. coli strains were isolated from urinary tract infections and the presence of 3 siderophore genes were analyzed using PCR among them...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28491244/diarrheagenic-escherichia-coli-pathotypes-frequency-in-khuzestan-province-of-iran
#10
Atieh Darbandi, Parviz Owlia, Saeid Bouzari, Horieh Saderi
BACKGROUND AND OBJECTIVES: Diarrheagenic Escherichia coli (DEC) is an emerging agent among pathogens that causes diarrhea. Studies showed that diarrheagenic E. coli such as enterohaemorrhagic E. coli (EHEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), diffusely adhering E. coli (DAEC) and shiga toxin producing E. coli (STEC) strains are among the most frequent causative agents in acute diarrhea. The aim of this study was to determine the frequency of DEC pathotypes in Khuzestan province...
December 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149497/professor-caterina-mammina-1957-2016
#11
EDITORIAL
Mohammad Mehdi Feizabadi, Giovanni M Giammanco, Reza Ranjbar
No abstract text is available yet for this article.
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149496/pseudomoniasis-phytotherapy-a-review-on-most-important-iranian-medicinal-plants-effective-on-pseudomonas-aeruginosa
#12
REVIEW
Mahmoud Bahmani, Mahmoud Rafieian-Kopaei, Hassan Hassanzadazar, Morovat Taherikalani
BACKGROUND AND OBJECTIVES: Pseudomonas aeruginosa is a Gram-negative, aerobic bacterium found in water and soil. It is a normal flora in skin and gastrointestinal tract of human beings. P. aeruginosa as an opportunistic pathogen involved in nosocomial infections having multiple pathogenic factors and shows high rate of resistance to different antibiotics. The aim of this study was to identify the most important native medicinal plants of Iran effective on P. aeruginosa. MATERIALS AND METHODS: All required information was obtained by searching keywords such as P...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149495/molecular-typing-methods-used-in-studies-of-mycobacterium-tuberculosis-in-iran-a-systematic-review
#13
REVIEW
Hassan Ravansalar, Keyvan Tadayon, Kiarash Ghazvini
BACKGROUND AND OBJECTIVES: Molecular typing methods are important and useful tools to assess the transmission, diversity of strains and differentiation between new infections and relapses which can effectively help in controlling infections. The aim of this study was to evaluate the molecular typing methods which have been used in Iran. By evaluating the results and discriminatory power of each method, we can assign appropriate weight to each technique and ultimately offer a common strategy for future epidemiological studies...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149494/cloning-expression-and-immunoreactivity-of-recombinant-toxoplasma-gondii-gra5-protein
#14
Zahra Arab-Mazar, Shirzad Fallahi, Ameneh Koochaki, Hadi Mirahmadi, Seyyed Javad Seyyed Tabaei
BACKGROUND AND OBJECTIVES: Toxoplasma gondii is an obligatory intracellular parasite which causes severe diseases in the fetus of pregnant women and immunocopmromised patients. Serological tests based on recombinant protein are one of the main diagnosis methods for the detection of specific antibodies in serum samples. Dense granule antigenic proteins derived from T. gondii (TgGRAs) are potential antigens for the development of diagnostic tools. MATERIALS AND METHODS: DNA was extracted from T...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149493/prevalence-of-colonization-and-mitochondrial-large-subunit-rrna-mutation-of-pneumocystis-jiroveci-among-iranian-children
#15
Parisa Badiee, Ali Rezapour, Amin Abbasian, Hamid Reza Foroutan, Hadis Jafarian
BACKGROUND AND OBJECTIVES: Pneumocystis jiroveci is an important causative agent of Pneumocystis pneumonia. During childhood, exposure to Pneumocystis occurs and antibody was built in early childhood. The aim of this study was to describe the molecular epidemiology of P. jiroveci in children without any respiratory syndrome and survey the distribution of different mitochondrial large subunit, ribosomal ribonucleic acid (mtLSU- rRNA) genotypes. MATERIALS AND METHODS: Mini-bronchoalveolar lavage (mini-BAL) fluids from pediatric patients with no history of lung disorders were obtained during a 14-month period...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149492/distribution-of-hepatitis-c-virus-genotypes-in-arak-city-central-province-of-iran
#16
Masoomeh Sofian, Amitis Ramezani, Hossein Imani, Ali Asghar Farazi, Mohammad Banifazl, Ali Jourabchi, Arezoo Aghakhani
BACKGROUND AND OBJECTIVES: Hepatitis C virus (HCV) infection is a worldwide concern and it is the major cause of liver disease. Several genotypes of the HCV have been reported from different regions of the world. The determination of the HCV genotypes is important for the prediction of response to antiviral treatment and clinical outcomes. So, HCV genotyping in each region is of great importance. This investigation was performed to determine the distribution of HCV genotypes in Arak city, Central province of Iran...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149491/evidence-of-human-coroanvirus-229e-in-patients-with-respiratory-infection-iran-2015-the-first-report
#17
Ali Madhi, Arash Ghalyanchilangeroudi, Mohammad Soleimani
BACKGROUND AND OBJECTIVES: Human coronaviruses (HCoVs) are one of the main causes of upper respiratory tract infections in humans. While more often responsible for mild illness, they have been associated with illnesses that require hospitalization. MATERIALS AND METHODS: 270 Samples from patients hospitalized with the respiratory infection during the autumn season of 2015 were evaluated for the presence of four HCoVs (OC43, 229E, HUK1, and NL63) using an optimized SYBR green RT-PCR assay...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149490/occurrence-of-chlamydophila-felis-feline-herpesvirus-1-and-calcivirus-in-domestic-cats-of-iran
#18
Nadi Maazi, Shahram Jamshidi, Payman Kayhani, Hassan Momtaz
BACKGROUND AND OBJECTIVES: Feline herpesvirus-1, feline calicivirus and Chlamydophila felis are the main causes of feline upper respiratory tract disease. This study was conducted to identify of FeHV-1, FCV and C. felis infections in domestic cat population and also to estimate the prevalence of each specific infection in Iran. MATERIALS AND METHODS: The ocular conjunctiva and oropharyngeal specimens obtained from 80 cats were examined using PCR and reverse transcription PCR...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149489/a-comparison-of-antibiotic-disks-from-different-sources-on-quicolor-and-mueller-hinton-agar-media-in-evaluation-of-antibacterial-susceptibility-testing
#19
Neda Saffari, Siavosh Salmanzadeh-Ahrabi, Ahya Abdi-Ali, Mohsen Rezaei-Hemami
BACKGROUND AND OBJECTIVES: Antibacterial susceptibility testing of clinical bacterial isolates through disk diffusion method plays a major role in antibacterial treatment. One of the main factors affecting the result of these tests is the type, structure and quality of the disks. The main objective of this study was to compare the agreement of antibiotic disks originated from three companies on Quicolor and Mueller-Hinton agar. MATERIALS AND METHODS: Quicolor and Mueller-Hinton agar media were used in disk diffusion method...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28149488/multi-locus-sequence-type-analysis-of-shigellas-pp-isolates-from-tehran-iran
#20
Shadi Shahsavan, Maliheh Nobakht, Abdolaziz Rastegar-Lari, Parviz Owlia, Bita Bakhshi
BACKGROUND AND OBJECTIVES: Strains of Shigella spp. can cause shigellosis, or bacillary dysentery. that is a public health problem worldwide. The aim of this study was to describe the population structure and genetic relatedness of multidrug resistant S. sonnei and S. flexneri isolated during a one year period from children with diarrhea in Tehran, Iran. MATERIALS AND METHODS: A total of 70 Shigella spp. were detected during the study period. Twenty MDR isolates of Shigella spp...
October 2016: Iranian Journal of Microbiology
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