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Iranian Journal of Microbiology

Kiran Iqbal Masood, Bushra Jamil, Maryam Rahim, Muniba Islam, Muhammad Farhan, Zahra Hasan
Background and Objectives: Dengue virus infections (Dengue) have become increasingly common in Pakistan and can result in case fatalities if not managed appropriately. Patients with Dengue virus infection may be asymptomatic or present with Dengue fever (DF), Dengue with warning signs (DWS) or severe Dengue (SD). Severity in Dengue is coincident with an exacerbated production of lymphocyte-induced cytokines and chemokines which are associated with plasma leakage. We investigated the association of circulating levels of cytokines such as Interleukin (IL)-6, tumor necrosis factor (TNF)-alpha and CXCL-10 in Dengue patients with differing severity of disease...
June 2018: Iranian Journal of Microbiology
Maryam Yousefi, Ahmad Nejati, Seyed Mohsen Zahraei, Sussan Mahmoudi, Najmeh Parhizgari, Seyed Mohammad Jazayeri Farsani, Mahmood Mahmoodi, Rakhshandeh Nategh, Shohreh Shahmahmoodi
Background and Objectives: Acute flaccid paralysis (AFP) is a complicated clinical syndrome with a wide range of potential etiologies. Several infectious agents including different virus families have been isolated from AFP cases. In most surveys, Non-polio Enteroviruses (NPEVs) have been detected as main infectious agents in AFP cases; however, there are also some reports about Adenovirus isolation in these patients. In this study, NPEVs and Adenoviruses in stool specimens of AFP cases with or without Residual Paralysis (RP) with negative results for poliovirus are investigated...
June 2018: Iranian Journal of Microbiology
Marzieh Sobhani, Mahdi Abbas-Mohammadi, Samad Nejad Ebrahimi, Atousa Aliahmadi
Background and Objectives: Due to the importance of finding new and more effective antifungal and antibacterial compounds against invasive vaginitis strains, this study was conducted for fast screening of plant samples. Materials and Methods: Thirty Iranian plant samples were successively extracted by n -hexane, ethyl acetate and methanol to obtain a total of 90 extracts. Each extract was prepared in six concentrations and evaluated for antifungal activity via a micro-broth dilution method...
June 2018: Iranian Journal of Microbiology
Mohaddeseh Ramezani, Seyed Masoud Hosseini, Seyed Abolhassan Shahzadeh Fazeli, Mohammad Ali Amozegar, Javad Fakhari
Background and Objectives: The microbial communities of traditional milk-based food are of great importance in its manufacturing process, especially when using raw milk with natural cultures. Liqvan (Lighvan or Levan) is a traditional Iranian buried cheese, which is made from raw ewe's milk without a starter addition. The aim of this study was to explore the fungal active population during this cheese manufacturing process by comparing DNA and RNA based culture independent method Denaturing Gradient Gel Electrophoresis (DGGE)...
June 2018: Iranian Journal of Microbiology
Sheida Mohammadi, Nima Hosseini Jazani, Mehri Kouhkan, Leila Ashrafi Babaganjeh
Background and Objectives: Bacterial resistance is an emerging public health problem worldwide. Metallic nanoparticles and nanoalloys open a promising field due to their excellent antimicrobial effects. The aim of the present study was to investigate the antibacterial effects of Ag-Cu nanoalloys, which were biosynthesized by Lactobacillus casei ATCC 39392, on some of the important bacterial pathogens, including Escherichia coli , Burkholderia cepacia, Listeria monocytogenes and Brucella abortus ...
June 2018: Iranian Journal of Microbiology
Amjad Ahmadi, Fariba Farhadifar, Masoome Rezaii, Farnaz Zandvakili, Fariba Seyedoshohadaei, Mozhdeh Zarei, Sholeh Shahgheibi, Rashid Ramazanzadeh, Daem Roshani
Background and Objectives: Group B Streptococcali (GBS) is an important factor in newborn deaths in developed and developing countries. Trichomoniasis is one of the most prevalent sexually transmitted diseases (STDs) in the world, which is caused by protozoan Trichomonas vaginalis (T. vaginalis) . The present study compares the frequency of GBS and T. vaginalis genital infections in pregnant women, women with spontaneous abortion, as well as its role in spontaneous abortion. Materials and Methods: In this case-control study, 109 women were included with spontaneous abortion with gestational ages between 11-20 weeks and 109 pregnant women with gestational ages between 35-37 weeks in Sanandaj, Iran...
June 2018: Iranian Journal of Microbiology
Mojtaba Mohseni, Fariba Rafiei, Ezzat Allah Ghaemi
Background and Objectives: Exfoliative toxins (ETs) of Staphylococcus aureus are the main reason of scalded skin syndrome in infants and young children. The aim of this study was to investigate the prevalence of eta, etb and etd genes in S. aureus. Materials and Methods: A total of 150 S. aureus isolates were collected from clinical specimens during the years 2014 to 2016 in the north of Iran. After confirmation of the species using standard diagnostic procedures, polymerase chain reaction was used for detection of the eta, etb and etd genes among the isolates...
June 2018: Iranian Journal of Microbiology
Saeid Bouzari, Elham Farhang, Seyed Mostafa Hosseini, Mohammad Yousef Alikhani
Background and Objectives: Diarrheal disease is still a major health problem in developing countries, where it is considered as one of the leading causes of morbidity and mortality especially in children. Escherichia coli is one of the important enteropathogenic bacteria which causes diarrhea in people. The aim of this study was to investigate the prevalence and antimicrobial resistance of shiga toxin-producing E. coli (STEC), Enterohaemorrhagic E. coli (EHEC), and Enteropathogenic E...
June 2018: Iranian Journal of Microbiology
Mahmoud Agholi, Akbar Safaei, Mani Ramzi, Gholam Reza Hatam, Jamal Sarvari
Background and Objectives: Cytomegalovirus (CMV) infection is the most common viral opportunistic infection causing gastrointestinal diseases such diarrhea and colitis in immunocompromised patients. The development and performance of a robust and sensitive PCR assay are usually evaluated to detect CMV DNA in human fecal specimens. In this study, our aim was to detect CMV DNA in stool samples taken from patients with HIV/AIDS, cancer, and transplant recipient patients with chronic and persistent diarrhea using a non-invasive method...
April 2018: Iranian Journal of Microbiology
Sunita Bundale, Jaya Singh, Deovrat Begde, Nandita Nashikkar, Avinash Upadhyay
Background and Objectives: Rare actinomycetes are a promising source of novel metabolites of pharmaceutical importance. The current study focussed on selective isolation of specific genera of rare actinomycetes and screening the isolates for biosynthetic genes particularly polyketide synthases (PKS) and non ribosomal peptide synthetases (NRPS). Materials and Methods: The soil samples were subjected to various pre-treatments like 1.5% phenol treatment, 0.3% chloramine T treatment, benzethonium chloride treatment, etc...
April 2018: Iranian Journal of Microbiology
Reyhaneh Moradi, Rahim Nosrati, Hamed Zare, Tahereh Tahmasebi, Horieh Saderi, Parviz Owlia
Background and Objectives: Probiotics are defined as live micro-organisms conferring a health benefit on the host. Although most probiotics are bacteria, some yeasts such as Saccharomyces and Kluyveromyces , has been found to have effective probiotic properties. The objective of this study was to isolate and identify indigenous Saccharomyces and Kluyveromyces yeast strains and to compare some probiotic characteristics between these two strains in vitro . Materials and Methods: Strains were isolated on yeast glucose chloramphenicol agar medium from 205 samples and identified by morphological, physiological and biochemical assays...
April 2018: Iranian Journal of Microbiology
Rouholah Keshavarz, Nader Mosavari, Keyvan Tadayon, Masoud Haghkhah
Background and Objectives: Paratuberculosis (PTb) (John's disease) is an incurable chronic intestinal infection that mainly affects ruminants. PTb is caused by Mycobacterium avium subspecies paratuberculosis (MAP) with a global distribution. Despite evidences on MAP contribution in Crohn's disease its causal role is still a matter of controversy. In ruminant farming, vaccination is broadly accepted as an effective control measure of PTb. This article describes preparation and field trial of an inactivated PTb vaccine made from the MAP 316F strain...
April 2018: Iranian Journal of Microbiology
Katayon Berjis, Mahdieh Ghiasi, Sareh Sangy
Background and Objectives: There are very few analysis tools to examine seminal fluid specimens, so bacterial infections on male infertility has always been the subject of discussion. These infectious processes lead to deterioration of spermatogenesis, impairment of sperm function, and/or obstruction of the seminal tract. In this study, we aimed at determining the role of bacterial infection on semen parameters including motility, count and normal morphology in infertile male patients...
April 2018: Iranian Journal of Microbiology
Fariba Asgharpour, Seyed Mahmoud, Amin Marashi, Zahra Moulana
Background and Objectives: Multidrug resistant Salmonella strains have been observed around the world in recent years. Many mechanisms contribute to the spread of antimicrobial resistance genes. This study aimed at determining the distribution and transmission of class 1, 2 and 3 integrons among MDR Salmonella isolates collected from a selection of chicken broilers in the north of Iran. Materials and Methods: PCR assays were used to detect genes for tetracyclines ( tetA, tetB and tetG ), chloramphenicol ( cat1 and floR ), and streptomycin ( strA )...
April 2018: Iranian Journal of Microbiology
Sarah Jehad Ismail, Suhad Saad Mahmoud
Background and Objectives: Multidrug resistance and in particular, carbapenem resistant Gram-negative bacteria is spreading worldwide at an alarming rate. Among the clinically significant carbapenemases, the New Delhi Metallo-β-lactamase (NDM) is one of the most formidable. NDM efficiently hydrolyses β-lactams and is the last-resort among carbapenems. Hence, therapeutic options for NDM producer bacteria become restricted to a handful of antibiotics. The present study was undertaken to detect the prevalence of the bla NDM -variants Metallo β-lactamases (MBLs) among isolates of Pseudomonas aeruginosa recovered from various clinical samples of hospitalized patients in Baghdad, Iraq...
April 2018: Iranian Journal of Microbiology
Parisa Asadollahi, Fereshteh Jabalameli, Reza Beigverdi, Mohammad Emaneini
Background and Objectives: Variable number tandem repeat (VNTR) patterns and resistance against three commonly used hospital disinfectants [0.5% (w/w) chlorhexidine digluconate (CHG) and 75% (w/w) alcohol (A), CHG-A; Quaternary ammonium compounds (QACs) and biguanides (B), QAC-B; and 70% (w/w) isopropanol (ISP) and 0.25% (w/w) QACs, ISP-QAC], as well as frequently used antibiotics, were evaluated among 115 Staphylococcus epidermidis blood isolates recovered from a children's hospital in Tehran, Iran...
April 2018: Iranian Journal of Microbiology
Hossein Sedaghat, Bahram Nasr Esfahani, Mehrdad Halaji, Azhar Sallari Jazi, Sina Mobasherizadeh, Seyed Rohollah Havaei, Mohammad Emaneini, Seyed Asghar Havaei
Background and Objectives: Staphylococcus aureus is an important pathogen that can be colonized in the nose and increase the risk of spreading infections in hospitals. The present study aimed at determining the phenotypic and genotypic characterization of S. aureus strains isolated from patients and healthcare workers (HCWs) from a teaching hospital in Isfahan, Iran. Materials and Methods: This cross-sectional study was performed on 262 nasal swabs and 23 clinical isolates that were collected from a teaching hospital during February and April 2016...
April 2018: Iranian Journal of Microbiology
Mansour Goudarzi, Ashraf Mohabati Mobarez, Shahin Najar-Peerayeh, Mohsen Mirzaee
Background and Objectives: The antibiotic resistance among Enterococcus faecium strains has increased worldwide. Additionally, biofilm-forming isolates of E. faecium play an important role in human infections. This study was conducted to investigate the prevalence of virulence and antibiotic resistance genes between biofilm-producing and non-biofilm-producing E. faecium strains. Materials and Methods: In this study, 228 E. faecium isolates from clinical and environmental specimens were obtained from different wards of hospitals in Lorestan province (Iran)...
April 2018: Iranian Journal of Microbiology
Mehri Haeili, Mohammad Mehdi Feizabadi
No abstract text is available yet for this article.
April 2018: Iranian Journal of Microbiology
Shima Shokri, Vahid Karimi, Arash Ghalyanchi Langeroudi, Mehdi Vasfi Marandi, Masoud Hashamzadeh, Taha Zabihipetroudi, Hamideh Najafi, Farshad Tehrani
Background and Objectives: Different epidemiological studies have found that backyard chickens are a reservoir for poultry diseases. Most backyard chicken flocks have a poor level of biosecurity, which increases the risk of spread of diseases. In recent years, the number of backyard chickens has been on the rise in Iran. However, the health status of backyard flocks is still poorly documented. Thus, this study aimed at examining the seroprevalence of antibodies against infectious bronchitis virus (IBV) and molecular surveillance and genotyping of IBV among backyard chickens (without vaccination history) in Mazandaran province, North of Iran, 2014...
February 2018: Iranian Journal of Microbiology
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