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Iranian Journal of Microbiology

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https://www.readbyqxmd.com/read/29997755/a-survey-of-the-frequency-of-cytomegalovirus-associated-diarrhea-in-immunocompromised-patients-using-a-non-invasive-method
#1
Mahmoud Agholi, Akbar Safaei, Mani Ramzi, Gholam Reza Hatam, Jamal Sarvari
Background and Objectives: Cytomegalovirus (CMV) infection is the most common viral opportunistic infection causing gastrointestinal diseases such diarrhea and colitis in immunocompromised patients. The development and performance of a robust and sensitive PCR assay are usually evaluated to detect CMV DNA in human fecal specimens. In this study, our aim was to detect CMV DNA in stool samples taken from patients with HIV/AIDS, cancer, and transplant recipient patients with chronic and persistent diarrhea using a non-invasive method...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997754/culturable-rare-actinomycetes-from-indian-forest-soils-molecular-and-physicochemical-screening-for-biosynthetic-genes
#2
Sunita Bundale, Jaya Singh, Deovrat Begde, Nandita Nashikkar, Avinash Upadhyay
Background and Objectives: Rare actinomycetes are a promising source of novel metabolites of pharmaceutical importance. The current study focussed on selective isolation of specific genera of rare actinomycetes and screening the isolates for biosynthetic genes particularly polyketide synthases (PKS) and non ribosomal peptide synthetases (NRPS). Materials and Methods: The soil samples were subjected to various pre-treatments like 1.5% phenol treatment, 0.3% chloramine T treatment, benzethonium chloride treatment, etc...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997753/screening-and-characterization-of-in-vitro-probiotic-criteria-of-saccharomyces-and-kluyveromyces-strains
#3
Reyhaneh Moradi, Rahim Nosrati, Hamed Zare, Tahereh Tahmasebi, Horieh Saderi, Parviz Owlia
Background and Objectives: Probiotics are defined as live micro-organisms conferring a health benefit on the host. Although most probiotics are bacteria, some yeasts such as Saccharomyces and Kluyveromyces , has been found to have effective probiotic properties. The objective of this study was to isolate and identify indigenous Saccharomyces and Kluyveromyces yeast strains and to compare some probiotic characteristics between these two strains in vitro . Materials and Methods: Strains were isolated on yeast glucose chloramphenicol agar medium from 205 samples and identified by morphological, physiological and biochemical assays...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997752/effectiveness-of-an-inactivated-paratuberculosis-vaccine-in-iranian-sheep-flocks-using-the-mycobacterium-avium-subsp-paratuberculosis-316f-strain
#4
Rouholah Keshavarz, Nader Mosavari, Keyvan Tadayon, Masoud Haghkhah
Background and Objectives: Paratuberculosis (PTb) (John's disease) is an incurable chronic intestinal infection that mainly affects ruminants. PTb is caused by Mycobacterium avium subspecies paratuberculosis (MAP) with a global distribution. Despite evidences on MAP contribution in Crohn's disease its causal role is still a matter of controversy. In ruminant farming, vaccination is broadly accepted as an effective control measure of PTb. This article describes preparation and field trial of an inactivated PTb vaccine made from the MAP 316F strain...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997751/study-of-seminal-infection-among-an-infertile-male-population-in-qom-iran-and-its-effect-on-sperm-quality
#5
Katayon Berjis, Mahdieh Ghiasi, Sareh Sangy
Background and Objectives: There are very few analysis tools to examine seminal fluid specimens, so bacterial infections on male infertility has always been the subject of discussion. These infectious processes lead to deterioration of spermatogenesis, impairment of sperm function, and/or obstruction of the seminal tract. In this study, we aimed at determining the role of bacterial infection on semen parameters including motility, count and normal morphology in infertile male patients...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997750/molecular-detection-of-class-1-2-and-3-integrons-and-some-antimicrobial-resistance-genes-in-salmonella-infantis-isolates
#6
Fariba Asgharpour, Seyed Mahmoud, Amin Marashi, Zahra Moulana
Background and Objectives: Multidrug resistant Salmonella strains have been observed around the world in recent years. Many mechanisms contribute to the spread of antimicrobial resistance genes. This study aimed at determining the distribution and transmission of class 1, 2 and 3 integrons among MDR Salmonella isolates collected from a selection of chicken broilers in the north of Iran. Materials and Methods: PCR assays were used to detect genes for tetracyclines ( tetA, tetB and tetG ), chloramphenicol ( cat1 and floR ), and streptomycin ( strA )...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997749/first-detection-of-new-delhi-metallo-%C3%AE-lactamases-variants-ndm-1-ndm-2-among-pseudomonas-aeruginosa-isolated-from-iraqi-hospitals
#7
Sarah Jehad Ismail, Suhad Saad Mahmoud
Background and Objectives: Multidrug resistance and in particular, carbapenem resistant Gram-negative bacteria is spreading worldwide at an alarming rate. Among the clinically significant carbapenemases, the New Delhi Metallo-β-lactamase (NDM) is one of the most formidable. NDM efficiently hydrolyses β-lactams and is the last-resort among carbapenems. Hence, therapeutic options for NDM producer bacteria become restricted to a handful of antibiotics. The present study was undertaken to detect the prevalence of the bla NDM -variants Metallo β-lactamases (MBLs) among isolates of Pseudomonas aeruginosa recovered from various clinical samples of hospitalized patients in Baghdad, Iraq...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997748/assessment-of-disinfectant-and-antibiotic-susceptibility-patterns-and-multi-locus-variable-number-tandem-repeat-analysis-of-staphylococcus-epidermidis-isolated-from-blood-cultures
#8
Parisa Asadollahi, Fereshteh Jabalameli, Reza Beigverdi, Mohammad Emaneini
Background and Objectives: Variable number tandem repeat (VNTR) patterns and resistance against three commonly used hospital disinfectants [0.5% (w/w) chlorhexidine digluconate (CHG) and 75% (w/w) alcohol (A), CHG-A; Quaternary ammonium compounds (QACs) and biguanides (B), QAC-B; and 70% (w/w) isopropanol (ISP) and 0.25% (w/w) QACs, ISP-QAC], as well as frequently used antibiotics, were evaluated among 115 Staphylococcus epidermidis blood isolates recovered from a children's hospital in Tehran, Iran...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997747/genetic-diversity-of-staphylococcus-aureus-strains-from-a-teaching-hospital-in-isfahan-iran-the-emergence-of-mrsa-st639-scc-mec-iii-and-st343-scc-mec-iii
#9
Hossein Sedaghat, Bahram Nasr Esfahani, Mehrdad Halaji, Azhar Sallari Jazi, Sina Mobasherizadeh, Seyed Rohollah Havaei, Mohammad Emaneini, Seyed Asghar Havaei
Background and Objectives: Staphylococcus aureus is an important pathogen that can be colonized in the nose and increase the risk of spreading infections in hospitals. The present study aimed at determining the phenotypic and genotypic characterization of S. aureus strains isolated from patients and healthcare workers (HCWs) from a teaching hospital in Isfahan, Iran. Materials and Methods: This cross-sectional study was performed on 262 nasal swabs and 23 clinical isolates that were collected from a teaching hospital during February and April 2016...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997746/prevalence-of-biofilm-formation-and-vancomycin-resistant-genes-among-enterococcus-faecium-isolated-from-clinical-and-environmental-specimens-in-lorestan-hospitals
#10
Mansour Goudarzi, Ashraf Mohabati Mobarez, Shahin Najar-Peerayeh, Mohsen Mirzaee
Background and Objectives: The antibiotic resistance among Enterococcus faecium strains has increased worldwide. Additionally, biofilm-forming isolates of E. faecium play an important role in human infections. This study was conducted to investigate the prevalence of virulence and antibiotic resistance genes between biofilm-producing and non-biofilm-producing E. faecium strains. Materials and Methods: In this study, 228 E. faecium isolates from clinical and environmental specimens were obtained from different wards of hospitals in Lorestan province (Iran)...
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29997745/the-threat-of-colistin-resistance-among-carbapenem-resistant-klebsiella-pneumoniae-isolates-in-iran
#11
EDITORIAL
Mehri Haeili, Mohammad Mehdi Feizabadi
No abstract text is available yet for this article.
April 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922421/seroprevalence-and-genotyping-of-avian-infectious-bronchitis-virus-detected-from-iranian-unvaccinated-backyard-chickens
#12
Shima Shokri, Vahid Karimi, Arash Ghalyanchi Langeroudi, Mehdi Vasfi Marandi, Masoud Hashamzadeh, Taha Zabihipetroudi, Hamideh Najafi, Farshad Tehrani
Background and Objectives: Different epidemiological studies have found that backyard chickens are a reservoir for poultry diseases. Most backyard chicken flocks have a poor level of biosecurity, which increases the risk of spread of diseases. In recent years, the number of backyard chickens has been on the rise in Iran. However, the health status of backyard flocks is still poorly documented. Thus, this study aimed at examining the seroprevalence of antibodies against infectious bronchitis virus (IBV) and molecular surveillance and genotyping of IBV among backyard chickens (without vaccination history) in Mazandaran province, North of Iran, 2014...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922420/serological-and-molecular-characterization-of-hepatitis-b-virus-in-asymptomatic-blood-donors-in-iran
#13
Mona Seyed Attaran, Seyed Masoud Hosseini, Javad Fakhari, Zohreh Sharifi
Background and Objectives: Hepatitis B virus (HBV) is an enveloped DNA virus belongs to Hepadnaviridea family. HBV infection is a serious global health problem, with 2 billion people infected worldwide, and 350 million suffering from chronic HBV infection. The aim of this study was to determine the serological pattern and molecular characterization of HBV diversity in asymptomatic blood donors in Iran alongside with the mutation status of HBV in relation to blood safety. Materials and Methods: One hundred and sixty six samples from asymptomatic blood donors who were positive for hepatitis B surface antigen during 2012 to 2014 were selected...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922419/antimicrobial-effect-of-licochalcone-a-and-epigallocatechin-3-gallate-against-salmonella-typhimurium-isolated-from-poultry-flocks
#14
Somayyeh Hosseinzadeh, Habib Dastmalchi Saei, Malahat Ahmadi, Taghi Zahraei Salehi
Background and Objectives: Salmonellosis due to multi-drug resistant Salmonella Typhimurium with biofilm formation ability is a serious public health threat worldwide. Studies have shown that medicinal plants inhibit the growth of bacterial species. The present study aimed at determining antibiotic resistance pattern and biofilm formation ability of S. Typhimurium isolated from poultry flocks. Moreover, the antibacterial activity of Licochalcone A (LAA) and Epigallocatechin-3-gallate (EGCG) against the studied isolates were investigated in this study...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922418/-salmonella-enteritidis-and-salmonella-typhimorium-identification-in-poultry-carcasses
#15
Asma Afshari, Ahmad Baratpour, Saeed Khanzade, Abdollah Jamshidi
Background and Objectives: Salmonellosis caused by Salmonella spp. is one of the most important zoonotic diseases and transmits to human through raw food animal products including poultry meat. Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium are the most important strains that infect human. This study was conducted to evaluate the contamination rate of poultry carcasses with S. Enteritidis and S. Typhimurium using multiplex PCR assay. Materials and Methods: 100 samples were selected during the summer and fall of 2010 by cluster sampling method from 10 broiler flocks, which were slaughtered in a poultry abattoir located in Mashhad suburb...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922417/direct-disk-testing-versus-isolation-and-antimicrobial-susceptibility-testing-of-urine-from-urinary-tract-infection
#16
Raz Nawzad Mohammad, Sherko Ali Omer
Background and Objectives: Urinary tract infections are common infections that can be caused by many bacterial pathogens. The susceptibility of such pathogens to antimicrobial agents is identified by different methods including disk diffusion test, direct sensitivity testing and determination minimum inhibitory concentration. The present study was conducted to isolate and identify bacteria cultured from urine samples and compare the results of direct sensitivity test (DST) against Kirby-Bauer's disk diffusion antimicrobial sensitivity (AST) with respect to reliability, time and cost...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922416/mlva-typing-of-haemophilus-influenzae-isolated-from-two-iranian-university-hospitals
#17
Faranak Nejati, Abolfazl Fateh, Seyed Ali Nojoumi, Mohammad Rahbar, Ava Behrouzi, Farzam Vaziri, Seyed Davar Siadat
Background and Objectives: Different serotypes of Haemophilus influenzae is now divided into 2 divisions: encapsulated and unencapsulated. Multiple locus variable number tandem repeat analysis (MLVA) includes such specifications as the extra power of separation, ease of data interpretation, and epidemiological data accordance, which have made it an appropriate molecular device for good typing and phylogenetic analysis of bacterial pathogens. Materials and Methods: In this research, cultured samples were studied and strains identified through biochemical tests were recognized...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922415/gene-cassettes-of-class-i-integron-associated-with-antimicrobial-resistance-in-isolates-of-citrobacter-spp-with-multidrug-resistance
#18
Roya Chegene Lorestani, Alisha Akya, Azam Elahi, Yazdan Hamzavi
Background and Objectives: Integrons play a major role in the transmission and accumulation of resistance factors in multidrug resistant bacteria. This study was aimed to evaluate the gene cassettes of class I integron and antimicrobial resistance in isolates of Citrobacter with multidrug resistance (MDR). Materials and Methods: Ninety isolates of Citrobacter spp. were collected from the largest hospital in Kermanshah, Iran. Antimicrobial resistance patterns were determined using disc diffusion method...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922414/molecular-assessment-of-accessory-gene-regulator-agr-quorum-sensing-system-in-biofilm-forming-staphylococcus-aureus-and-study-of-the-effect-of-silver-nanoparticles-on-agr-system
#19
Ahmed Mohammed Turkey, Khadija Khalil Barzani, Ahmed Abdul Jabbar Suleiman, Jenan Jameel Abed
Background and Objectives: Staphylococcus aureus is an opportunistic human pathogen that causes a variety of diseases. Staphylococcal biofilms are a source of chronic and continual infections. This study was conducted to estimate the distribution of agr among different isolates of S. aureus and their relationship with biofilm. Also, it was aimed to check the association of operon agr with virulence factors ( seb, eta, spa and tst v8 ) and study the effect of biosynthesis silver nanoparticles on the function of the agr system...
February 2018: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29922413/distribution-of-staphylococcal-cassette-chromosome-mec-types-among-methicillin-resistant-coagulase-negative-staphylococci-in-central-iran
#20
Ehsanollah Ghaznavi-Rad, Nasimeh Fard-Mousavi, Ali Shahsavari, Ali Japoni-Nejad, Alex Van Belkum
Background and Objectives: Methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are important nosocomial pathogens. They may serve as a reservoir of SCC mec , the genomic island encoding amongst other methicillin resistance. This study was designed to determine the distribution of different SCC mec types from MR-CoNS isolated from clinical specimens in a tertiary hospital in central Iran, having high frequency of nosocomial methicillin-resistant staphylococcal infections. Materials and Methods: We evaluated isolates from patients attending the Vali-Asr Hospital located in the center of Iran, from February to December 2012...
February 2018: Iranian Journal of Microbiology
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