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International Journal of Microbiology

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https://www.readbyqxmd.com/read/28912815/feasibility-of-metatranscriptome-analysis-from-infant-gut-microbiota-adaptation-to-solid-foods-results-in-increased-activity-of-firmicutes-at-six-months
#1
Floor Hugenholtz, Jarmo Ritari, Lotta Nylund, Mark Davids, Reetta Satokari, Willem M de Vos
Newborns are rapidly colonized by microbes and their intestinal tracts contain highly dynamic and rapidly developing microbial communities in the first months of life. In this study, we describe the feasibility of isolating mRNA from rapidly processed faecal samples and applying deep RNA-Seq analysis to provide insight into the active contributors of the microbial community in early life. Specific attention is given to the impact of removing rRNA from the mRNA on the phylogenetic and transcriptional profiling and its analysis depth...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28831285/prevalence-of-bacterial-vaginosis-and-associated-risk-factors-among-women-complaining-of-genital-tract-infection
#2
Adane Bitew, Yeshiwork Abebaw, Delayehu Bekele, Amete Mihret
BACKGROUND: Bacterial vaginosis is a global concern due to the increased risk of acquisition of sexually transmitted infections. OBJECTIVES: To determine the prevalence of bacterial vaginosis and bacteria causing aerobic vaginitis. METHODS: A cross-sectional study was conducted among 210 patients between September 2015 and July 2016 at St. Paul's Hospital. Gram-stained vaginal swabs were examined microscopically and graded as per Nugent's procedure...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28814955/characterization-of-carbapenem-resistant-acinetobacter-baumannii-strains-isolated-from-hospitalized-patients-in-palestine
#3
Regeen Handal, Lulu Qunibi, Ibrahim Sahouri, Maha Juhari, Rula Dawodi, Hiyam Marzouqa, Musa Hindiyeh
The American Centers for Disease Control and Prevention (CDC) recognizes Acinetobacter baumannii as a source of global outbreaks and epidemics especially due to its increasing resistance to commercially available antibiotics. In this study, 69 single patient multidrug resistant isolates collected from all over Palestine, except Gaza, were studied. All the isolates were resistant to all the β-lactam antibiotics including the carbapenems. Of the 69 isolates, 82.6% were positive for blaOXA-23, 14.5% were positive for blaOXA-24, and 3% were positive for blaOXA-58...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28757872/production-characterization-and-antimicrobial-activity-of-mycocin-produced-by-debaryomyces-hansenii-dsmz70238
#4
Safaa A S Al-Qaysi, Halah Al-Haideri, Zaid Akram Thabit, Wijdan Hameed Abd Al-Razzaq Al-Kubaisy, Jamal Abd Al-Rahman Ibrahim
The present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by D. hansenii DSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicrobial activity were studied. The results showed that the optimum inhibitory effect of killer toxin was at 8% NaCl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Candida albicans, and Candida neoformans, respectively...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28751915/gene-expression-and-molecular-characterization-of-a-xylanase-from-chicken-cecum-metagenome
#5
Hind Al-Darkazali, Vithaya Meevootisom, Duangnate Isarangkul, Suthep Wiyakrutta
A xylanase gene xynAMG1 with a 1,116-bp open reading frame, encoding an endo-β-1,4-xylanase, was cloned from a chicken cecum metagenome. The translated XynAMG1 protein consisted of 372 amino acids including a putative signal peptide of 23 amino acids. The calculated molecular mass of the mature XynAMG1 was 40,013 Da, with a theoretical pI value of 5.76. The amino acid sequence of XynAMG1 showed 59% identity to endo-β-1,4-xylanase from Prevotella bryantii and Prevotella ruminicola and 58% identity to that from Prevotella copri...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28676828/prevalence-of-virulence-genes-associated-with-diarrheagenic-pathotypes-of-escherichia-coli-isolates-from-water-sediment-fish-and-crab-in-aby-lagoon-c%C3%A3-te-d-ivoire
#6
Ollo Kambire, Ama Antoinette Adingra, Konan Mathurin Yao, Rose Koffi-Nevry
This study was conducted to characterize virulence genes of Escherichia coli isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 E. coli strains. The presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) was assessed by multiplex PCR using specific primers. Based on the multiplex PCR, sixty-two isolates (42 from water, 19 from sediment, and 1 from crab) were positive for virulence genes, including 34 positive for elt (ETEC), 46 positive for est (ETEC), 24 positive for both elt and est, 6 positive for stx (EHEC), 1 positive for both stx + est, and 1 positive for both stx + elt...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28676827/isolation-and-characterization-of-yeasts-able-to-assimilate-sugarcane-bagasse-hemicellulosic-hydrolysate-and-produce-xylitol-associated-with-veturius-transversus-passalidae-coleoptera-and-insecta
#7
Italo Thiago Silveira Rocha Matos, Enedina Nogueira Assunção, Edson Junior do Carmo, Verena Makaren Soares, Spartaco Astolfi-Filho
Yeasts are an important component of insect gut microbial content, playing roles such as degradation of polymers and toxic compounds, biological control, and hormone, vitamin, and digestive enzyme production. The xylophagous beetle gut is a hyperdiverse habitat and a potential source of new species with industrial abilities such as enzyme production, pentose fermentation, and biodetoxification. In this work, samples of Veturius transversus (Passalidae, Coleoptera, and Insecta) were collected from the Central Amazon Rainforest...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28638412/molecular-identification-of-aminoglycoside-modifying-enzymes-and-plasmid-mediated-quinolone-resistance-genes-among-klebsiella-pneumoniae-clinical-isolates-recovered-from-egyptian-patients
#8
Mohamed F El-Badawy, Wael M Tawakol, Shaymaa W El-Far, Ibrahim A Maghrabi, Saleh A Al-Ghamdi, Moselhy S Mansy, Mohammed S Ashour, Mohamed M Shohayeb
Inappropriate use of antibiotics in clinical settings is thought to have led to the global emergence and spread of multidrug-resistant pathogens. The goal of this study was to investigate the prevalence of genes encoding aminoglycoside resistance and plasmid-mediated quinolone resistance among clinical isolates of Klebsiella pneumoniae. All K. pneumoniae isolates were phenotypically identified using API 20E and then confirmed genotypically through amplification of the specific K. pneumoniae phoE gene. All isolates were genotyped by the enterobacterial repetitive intergenic consensus polymerase chain reaction technique (ERIC-PCR)...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28630628/staphylococcus-saprophyticus-recovered-from-humans-food-and-recreational-waters-in-rio-de-janeiro-brazil
#9
Viviane Santos de Sousa, Ana Paula de Souza da-Silva, Leif Sorenson, Raphael Paiva Paschoal, Renata Fernandes Rabello, Eloiza Helena Campana, Márcia Soares Pinheiro, Lyssa Oliveira Ferreira Dos Santos, Natacha Martins, Ana Carolina Nunes Botelho, Renata Cristina Picão, Sérgio Eduardo Longo Fracalanzza, Lee Woodland Riley, George Sensabaugh, Beatriz Meurer Moreira
Staphylococcus saprophyticus is an important agent of urinary tract infection (UTI) in young women, but information about this pathogen in human microbiota and in common environment is lacking. The aim of this study was to characterize S. saprophyticus isolates from genitoanal microbiota of 621 pregnant women, 10 minas cheese packs, and five beaches in Rio de Janeiro city and compare PFGE profiles of these isolates with five UTI PFGE clusters described in this city. We investigated 65 S. saprophyticus isolates from microbiota, 13 from minas cheese, and 30 from beaches and 32 UTI isolates...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28596790/high-doses-of-halotolerant-gut-indigenous-lactobacillus-plantarum-reduce-cultivable-lactobacilli-in-newborn-calves-without-increasing-its-species-abundance
#10
Alexander Rodriguez-Palacios, Henry R Staempfli, J Scott Weese
To elucidate the ecological effect of high oral doses of halotolerant (resistant to table salt) indigenous-gut bacteria on other commensals early in life, we conducted a culture-based study to quantify the effect of intestinal Lactobacillus plantarum strain of bovine origin (with remarkable aerobic growth capabilities and inhibitory activity against Escherichia coli O157:H7 and F5) on clinical health and gut lactobacilli/coliforms in newborn calves. In a double-blind placebo-randomized trial twelve colostrum-fed calves, consecutively born at a farm, were fed L...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28584528/pigeon-pea-and-cowpea-based-cropping-systems-improve-vesicular-arbuscular-mycorrhizal-fungal-colonisation-of-subsequent-maize-on-the-alfisols-in-central-malawi
#11
Keston O W Njira, Ernest Semu, Jerome P Mrema, Patson C Nalivata
Mycorrhizal associations contribute to the sustainability of crop production systems through their roles in nutrient cycling and other benefits in the soil-plant ecosystems. A two-year study was conducted on the Alfisols of Lilongwe and Dowa districts, Central Malawi, to assess the vesicular-arbuscular mycorrhizal (VAM) fungal colonisation levels in pigeon pea, cowpea, and maize grown in sole cropping, legume-cereal, and legume-legume intercropping systems and in the maize grown in short rotation (year 2) as influenced by the previous cropping systems and N fertilizer application...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28567057/molecular-study-of-acinetobacter-baumannii-isolates-for-metallo-%C3%AE-lactamases-and-extended-spectrum-%C3%AE-lactamases-genes-in-intensive-care-unit-mansoura-university-hospital-egypt
#12
Nashwa M Alkasaby, Maysaa El Sayed Zaki
Acinetobacter baumannii (A. baumannii) has been known as a causative pathogen of hospital acquired infections. The aim of this study is to examine the presence of A. baumannii among clinical isolates from intensive care unit (ICU) in Mansoura University Hospital (MUH), its antibiotic resistance pattern, and prevalence of antibiotic resistance genes metallo-β-lactamases (MBLs) and extended-spectrum-β-lactamases (ESBLs) among A. baumannii isolates. A. baumannii was identified by colony morphology, API 20E, and confirmed by detecting the bla OXA-51-like carbapenemase gene by PCR...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28546811/antimicrobial-activity-of-a-cationic-guanidine-compound-against-two-pathogenic-oral-bacteria
#13
E Escamilla-García, A G Alcázar-Pizaña, J C Segoviano-Ramírez, C Del Angel-Mosqueda, A P López-Lozano, E Cárdenas-Estrada, M A De La Garza-Ramos, C E Medina-De La Garza, M Márquez
This study evaluated the potential antimicrobial properties of a polyguanidine (CatDex) on two oral bacteria. Chlorhexidine gluconate 1340 μmoL L(-1) (CHX 0.12%) was used as control. Streptococcus mutans (S. mutans) and Porphyromonas gingivalis (P. gingivalis) were grown in BHI media. Bacterial sensitivity and antimicrobial activity were determined by the minimum inhibitory concentration (MIC) and Kirby-Bauer methods. To study side effects, that is, toxicity, dental pulp stem cells (DPSCs) were used. Fluorometric cytotoxicity and confocal microscopy assays were used in order to test cell viability...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28539938/effects-of-medicinal-plant-extracts-and-photosensitization-on-aflatoxin-producing-aspergillus-flavus-raper-and-fennell
#14
Loise M Njoki, Sheila A Okoth, Peter M Wachira
This study was undertaken with an aim of exploring the effectiveness of medicinal plant extracts in the control of aflatoxin production. Antifungal properties, photosensitization, and phytochemical composition of aqueous and organic extracts of fruits from Solanum aculeastrum, bark from Syzygium cordatum, and leaves from Prunus africana, Ocimum lamiifolium, Lippia kituiensis, and Spinacia oleracea were tested. Spores from four-day-old cultures of previously identified toxigenic fungi, UONV017 and UONV003, were used...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28484494/antimicrobial-resistance-of-salmonella-enterica-serovars-typhi-and-paratyphi-isolates-from-a-general-hospital-in-karawaci-tangerang-indonesia-a-five-year-review
#15
Nata Pratama Hardjo Lugito, Cucunawangsih
Typhoid and paratyphoid fever known as enteric fever pose important global public health problem, with 21.6 million cases and approximately 250,000 deaths annually. It is a prevalent disease in Indonesia, but data on the antimicrobial resistance pattern is limited. This study aim was to provide data on the antimicrobial resistance pattern of S. Typhi and S. Paratyphi bloodstream isolates in a general hospital in Karawaci, Tangerang, Banten, Indonesia, during the period of January 2011 to December 2015. Susceptibility against antimicrobials was detected according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI)...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28458692/control-of-passion-fruit-fungal-diseases-using-essential-oils-extracted-from-rosemary-rosmarinus-officinalis-and-eucalyptus-eucalyptus-agglomerata-in-egerton-university-main-campus-njoro-kenya
#16
Paul Njenga Waithaka, Eliud Mugu Gathuru, Benson Muriuki Githaiga, Salome Nduta Kimani
Growth of fruits which form an important part of human diet has been jeopardized by the many fungal diseases that are present today. This study was conceived to isolate the most common fungal pathogens in passion fruits. Fungi were isolated using potato dextrose agar in addition to characterization using morphological, cultural, and biochemical means. Extraction of essential oils from rosemary (Rosmarinus officinalis) and eucalyptus (Eucalyptus agglomerata) was done. Before carrying the sensitivity test of essential oils to the fungal isolates, constituents of the essential oils were determined...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28386280/bacterial-isolates-and-their-antimicrobial-susceptibility-patterns-of-wound-infections-among-inpatients-and-outpatients-attending-the-university-of-gondar-referral-hospital-northwest-ethiopia
#17
Aynalem Mohammed, Mengistu Endris Seid, Teklay Gebrecherkos, Moges Tiruneh, Feleke Moges
Background. The widespread uses of antibiotics, together with the length of time over which they have been available, have led to the emergence of resistant bacterial pathogens contributing to morbidity and mortality. This study was aimed to assess bacterial isolates and their drug susceptibility patterns from inpatients and outpatients with pus and/or wound discharge. Methods. A cross-sectional study was conducted at the University of Gondar Referral Hospital from March to May, 2014. Wound swab samples were collected from each study participant and inoculated into appropriate media...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28367217/bacteriological-profile-and-antimicrobial-susceptibility-patterns-of-bacteria-isolated-from-pus-wound-swab-samples-from-children-attending-a-tertiary-care-hospital-in-kathmandu-nepal
#18
Salu Rai, Uday Narayan Yadav, Narayan Dutt Pant, Jaya Krishna Yakha, Prem Prasad Tripathi, Asia Poudel, Binod Lekhak
In Nepal, little is known about the microbiological profile of wound infections in children and their antimicrobial susceptibility patterns. Total of 450 pus/wound swab samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. The antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion technique. Methicillin-resistant Staphylococcus aureus isolates were detected by using cefoxitin disc and confirmed by determining minimum inhibitory concentrations (MIC) of oxacillin...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28348597/prevalence-of-bacteriuria-and-antimicrobial-susceptibility-patterns-among-diabetic-and-nondiabetic-patients-attending-at-debre-tabor-hospital-northwest-ethiopia
#19
Seble Worku, Awoke Derbie, Mulusew Alemneh Sinishaw, Yesuf Adem, Fantahun Biadglegne
Background. Urinary tract infection is a major health problem especially in developing countries. Information about bacterial pathogens isolated from urinary tract infection in diabetic patients and their antimicrobial susceptibility patterns is limited in Ethiopia. Therefore, this study aimed at isolating bacterial pathogens and their antimicrobial susceptibility patterns. Methods. A hospital based comparative cross-sectional study was conducted at Debre Tabor. Urine sample was inoculated onto cysteine lysine electrolyte deficient (CLED) medium...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28270849/prevalence-of-extended-spectrum-beta-lactamases-producing-microorganisms-in-patients-admitted-at-krrh-southwestern-uganda
#20
Baguma Andrew, Atek Kagirita, Joel Bazira
The emergence of extended-spectrum beta-lactamase- (ESBL-) producing pathogenic bacteria at Kabale Regional Referral Hospital (KRRH), located in southwestern Uganda, is of great concern: a phenomenon that worries clinicians and other healthcare workers due to the serious threat they pose to patients. This current study aimed at determining the phenotypic detection of ESBL-producing strains of E. coli, Klebsiella sp., and Proteus sp. isolated from clinical specimens and their prevalence in patients admitted at KRRH...
2017: International Journal of Microbiology
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