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International Journal of Microbiology

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https://www.readbyqxmd.com/read/29721021/molecular-typing-and-antimicrobial-susceptibility-of-methicillin-resistant-staphylococcus-aureus-isolated-from-bovine-milk-in-tanzania
#1
Jibril Mohammed, Michael Henry Ziwa, Yaovi Mahuton Gildas Hounmanou, Adela Kisanga, Huruma Nelwike Tuntufye
Methicillin-resistant Staphylococcus aureus (MRSA) in raw milk can be transmitted from animals to humans, and in Tanzania raw milk is sold in local markets and consumed as purchased. This study was performed to determine the molecular characteristics and antimicrobial susceptibility pattern of MRSA strains isolated from raw bovine milk sold at local markets in Tanzania. A total of 117 raw milk samples were cultured on Baird-Parker medium to isolate S. aureus and PCR was used for amplification of gltB gene for S...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29692813/prevalence-of-bacterial-vaginosis-and-its-association-with-risk-factors-among-nonpregnant-women-a-hospital-based-study
#2
Eliza Ranjit, Bijendra Raj Raghubanshi, Smrity Maskey, Pramila Parajuli
Bacterial vaginosis (BV) is an ecological imbalance of the vaginal microbiota affecting mostly women of reproductive age group. This study was carried out among 160 nonpregnant women registered at the Outpatient Department of Gynaecology/Obstetrics of KIST Medical College Teaching Hospital, Imadol, Lalitpur, Nepal, from November 2014 to May 2015. The aim of the study was to assess the association of the risk factors with BV and analyze the type of bacteria associated with BV. Nugent's scoring method was used for diagnosis of BV in this study...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29681943/morphological-and-molecular-identification-of-the-causal-agent-of-anthracnose-disease-of-avocado-in-kenya
#3
S K Kimaru, E Monda, R C Cheruiyot, J Mbaka, A Alakonya
Anthracnose disease of avocado contributes to a huge loss of avocado fruits due to postharvest rot in Kenya. The causal agent of this disease has not been clear but presumed to be Colletotrichum gloeosporioides as reported in other regions where avocado is grown. The fungus mainly infects fruits causing symptoms such as small blackish spots, "pepper spots," and black spots with raised margin which coalesce as infection progresses. Due to economic losses associated with the disease and emerging information of other species of fungi as causal agents of the disease, this study was aimed at identifying causal agent(s) of the disease...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29681942/bacterial-profile-antibacterial-resistance-pattern-and-associated-factors-from-women-attending-postnatal-health-service-at-university-of-gondar-teaching-hospital-northwest-ethiopia
#4
Abebaw Bitew Kifilie, Mulat Dagnew, Birhanemeskel Tegenie, Biruk Yeshitela, Rawleigh Howe, Ebba Abate
Introduction: Surgical site infection is a vital cause of maternal mortality and morbidity, especially in resource-limited countries. The rise of antibiotic resistance bacterial infection poses a big threat to this vulnerable population. However, there is lack of studies around the study area. Objective: The purpose of this study was to identify bacterial profile, antibacterial resistance pattern, and associated factors among mothers attending postnatal care health service...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29675044/effects-of-bile-acids-and-nisin-on-the-production-of-enterotoxin-by-clostridium-perfringens-in-a-nutrient-rich-medium
#5
Miseon Park, Fatemeh Rafii
Clostridium perfringens is the second most common cause of bacterial foodborne illness in the United States, with nearly a million cases each year. C. perfringens enterotoxin (CPE), produced during sporulation, damages intestinal epithelial cells by pore formation, which results in watery diarrhea. The effects of low concentrations of nisin and bile acids on sporulation and toxin production were investigated in C. perfringens SM101, which carries an enterotoxin gene on the chromosome, in a nutrient-rich medium...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29666650/bacterial-community-of-the-rice-floodwater-using-cultivation-independent-approaches
#6
Michele Pittol, Erin Scully, Daniel Miller, Lisa Durso, Lidia Mariana Fiuza, Victor Hugo Valiati
In agricultural systems, interactions between plants and microorganisms are important to maintaining production and profitability. In this study, bacterial communities in floodwaters of rice fields were monitored during the vegetative and reproductive stages of rice plant development using 16S amplicon sequencing. The study was conducted in the south of Brazil, during the crop years 2011/12 and 2012/13. Comparative analyses showed strong differences between the communities of floodwaters associated with the two developmental stages...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29623093/prevalence-and-antibiotics-susceptibility-pattern-of-salmonella-and-shigella-species-among-diarrheal-patients-attending-nekemte-referral-hospital-oromia-ethiopia
#7
Alemayehu Terfassa, Mulissa Jida
The main objective of this study was determining the prevalence and antibiotics resistance pattern of Salmonella and Shigella sp. from diarrheal patients attending Nekemte Referral Hospital. A total of 422 patients were included in the study and their sociodemographic and clinical information was collected using questionnaire. Stool samples of the patients were collected and processed following standard bacteriological protocols. Presumptive colonies of Salmonella and Shigella species were identified and subcultured to their respective genera by standard biochemical tests...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29552038/assessment-of-two-years-sputum-smear-results-among-tuberculosis-suspected-patients-in-gambella-regional-hospital-western-ethiopia
#8
Mistire Wolde, Alemayehu Fereja
Background: Determining burden of pulmonary tuberculosis (PTb) may be associated with success of directly observed theraphy (DOT) services in high PTb areas, such as Gambella, Ethiopia. Objective: To assess burden of sputum smear positive SSP-PTb reports in Gambella Regional Hospital, in two consecutive years: mid 2013 to 2015. Methods: From March 2013 to February 2015, PTb suspected patients' sputum Acid Fast Bacilli test results were collected from laboratory registration book of Gambella Regional Hospital, western Ethiopia...
2018: International Journal of Microbiology
https://www.readbyqxmd.com/read/29463983/morphological-and-genetic-diversity-of-rhizobia-nodulating-cowpea-vigna-unguiculata-l-from-agricultural-soils-of-lower-eastern-kenya
#9
Damaris K Ondieki, Evans N Nyaboga, John M Wagacha, Francis B Mwaura
Limited nitrogen (N) content in the soil is a major challenge to sustainable and high crop production in many developing countries. The nitrogen fixing symbiosis of legumes with rhizobia plays an important role in supplying sufficient N for legumes and subsequent nonleguminous crops. To identify rhizobia strains which are suitable for bioinoculant production, characterization of rhizobia is a prerequisite. The objective of this study was to assess the morphological and genetic diversity of rhizobia that nodulates cowpea in agricultural soils of lower eastern Kenya...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29422915/epidemiological-features-of-clostridium-difficile-colonizing-the-intestine-of-jordanian-infants
#10
Eman N Abu-Khader, Eman F Badran, Asem A Shehabi
Clostridium difficile is commonly found in the intestine of infants without causing any disease. This study investigated the most important epidemiological features of C. difficile strains colonizing intestine of Jordanian infants. A total of 287 fecal samples were collected from infants admitted to the Jordan University Hospital (JUH) over the period of 2015. Samples were cultured for C. difficile and their growth was identified using microbiological culture and PCR. The overall C. difficile colonization rate among hospitalized and nonhospitalized infants was 37/287 (12...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29362565/-staphylococcus-aureus-and-escherichia-coli-in-curd-cheese-sold-in-the-northeastern-region-of-south-america
#11
Tamiles Barreto de Deus, Ludmilla Santana Soares Barros, Ricardo Mendes da Silva, Wanessa Karine da Silva Lima, Danuza das Virgens Lima, Adriana Dos Santos Silva
The present study evaluated the microbiological and sanitary quality of curd cheese sold on the beaches of the Itaparica Island, Brazil, and verified whether a correlation exists between the commercialization conditions and the microbiological data. The research was performed between December 2015 and March 2017. Sixty samples of rennet-containing cheese were collected to estimate the populations of mesophylls, psychrotrophic microorganisms, mold and yeast, Staphylococcus aureus , total coliforms, and Escherichia coli ...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29358951/persistence-of-toxic-activity-of-fermentation-extracts-from-bacillus-thuringiensis-var-israelensis-after-more-than-three-decades-of-storage
#12
Luis Jesús Galán-Wong, Rossana Gamiño-Hernández, David Fernández-Chapa, Graciela García-Díaz, Myriam A De La Garza-Ramos, Claudio Guajardo-Barbosa, Hugo Alberto Luna-Olvera
This study was carried out to determine the persistence of toxicity of fermentation extracts of Bacillus thuringiensis var. israelensis after more than three decades of storage. For this purpose, a population of Aedes aegypti was established. The mortality rate of 20 spore-crystal extracts purified using the acetone-lactose coprecipitation method was measured and evaluated by bioassays according to a modified WHO protocol. The extracts with the highest mortality rate were determined in triplicate by their LD50 and LD98 ...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29259629/qualitative-analysis-of-microbial-dynamics-during-anaerobic-digestion-of-microalgal-biomass-in-a-uasb-reactor
#13
Anna Doloman, Yousef Soboh, Andrew J Walters, Ronald C Sims, Charles D Miller
Anaerobic digestion (AD) is a microbiologically coordinated process with dynamic relationships between bacterial players. Current understanding of dynamic changes in the bacterial composition during the AD process is incomplete. The objective of this research was to assess changes in bacterial community composition that coordinates with anaerobic codigestion of microalgal biomass cultivated on municipal wastewater. An upflow anaerobic sludge blanket reactor was used to achieve high rates of microalgae decomposition and biogas production...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29234354/differential-microbial-diversity-in-drosophila-melanogaster-are-fruit-flies-potential-vectors-of-opportunistic-pathogens
#14
Luis A Ramírez-Camejo, Génesis Maldonado-Morales, Paul Bayman
Drosophila melanogaster has become a model system to study interactions between innate immunity and microbial pathogens, yet many aspects regarding its microbial community and interactions with pathogens remain unclear. In this study wild D. melanogaster were collected from tropical fruits in Puerto Rico to test how the microbiota is distributed and to compare the culturable diversity of fungi and bacteria. Additionally, we investigated whether flies are potential vectors of human and plant pathogens. Eighteen species of fungi and twelve species of bacteria were isolated from wild flies...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29234353/enteric-fever-caused-by-salmonella-enterica-serovars-with-reduced-susceptibility-of-fluoroquinolones-at-a-community-based-teaching-hospital-of-nepal
#15
Anjeela Bhetwal, Anjila Maharjan, Puspa Raj Khanal, Narayan Prasad Parajuli
Enteric fever continues to be an important public health problem especially in developing countries of the tropical region including Nepal. In this study, we aimed to investigate the incidence of enteric fever associated with Salmonella enterica and determine its antimicrobial susceptibilities to therapeutic antimicrobials in a community based teaching hospital of Nepal. A total of 2,304 blood samples from suspected enteric fever patients attending Manmohan Memorial Teaching Hospital were processed with standard microbiological methods for the isolation and identification of bacterial pathogens...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29213286/survival-of-five-strains-of-shiga-toxigenic-escherichia-coli-in-a-sausage-fermentation-model-and-subsequent-sensitivity-to-stress-from-gastric-acid-and-intestinal-fluid
#16
Tone Mari Rode, Anette McLeod, Ingrid Måge, Even Heir, Lars Axelsson, Askild L Holck
The ability of foodborne pathogens to exhibit adaptive responses to stressful conditions in foods may enhance their survival when passing through the gastrointestinal system. We aimed to determine whether Escherichia coli surviving stresses encountered during a model dry-fermented sausage (DFS) production process exhibit enhanced tolerance and survival in an in vitro gastrointestinal model. Salami sausage batters spiked with five E. coli isolates, including enterohaemorrhagic E. coli strains isolated from different DFS outbreaks, were fermented in a model DFS process (20°C, 21 days)...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29181028/comparison-of-the-performance-of-urinary-mycobacterium-tuberculosis-antigens-cocktail-esat6-cfp10-and-mpt64-with-culture-and-microscopy-in-pulmonary-tuberculosis-patients
#17
Dewi Kartika Turbawaty, Adhi Kristianto Sugianli, Arto Yuwono Soeroto, Budi Setiabudiawan, Ida Parwati
Pulmonary tuberculosis (TB) is a major global health problem and is one of the top 10 causes of death worldwide. Our study aimed to evaluate the performance of urinary Mycobacterium tuberculosis (Mtb) antigens cocktail (ESAT6, CFP10, and MPT64) compared with culture and microscopy. This descriptive cross-sectional study was conducted in Dr. Hasan Sadikin General Hospital, Bandung, from January 2014 to October 2016. A total of 141 pulmonary tuberculosis patients were included. Sputum samples were examined for acid-fast bacilli (ZN stain) and mycobacterial culture (LJ); the Mtb antigens cocktail was examined in the urine sample...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29163641/isolation-and-characterization-of-thermophilic-bacteria-from-jordanian-hot-springs-bacillus-licheniformis-and-thermomonas-hydrothermalis-isolates-as-potential-producers-of-thermostable-enzymes
#18
Balsam T Mohammad, Hala I Al Daghistani, Atef Jaouani, Saleh Abdel-Latif, Christian Kennes
The aim of this study was the isolation and characterization of thermophilic bacteria from hot springs in Jordan. Ten isolates were characterized by morphological, microscopic, biochemical, molecular, and physiological characteristics. Sequencing of the 16S rDNA of the isolates followed by BLAST search revealed that nine strains could be identified as Bacillus licheniformis and one isolate as Thermomonas hydrothermalis . This is the first report on the isolation of Thermomonas species from Jordanian hot springs...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29147114/antibiotic-resistant-bacterial-isolates-from-captive-green-turtles-and-in-vitro-sensitivity-to-bacteriophages
#19
Alessandro Delli Paoli Carini, Ellen Ariel, Jacqueline Picard, Lisa Elliott
This study aimed to test multidrug resistant isolates from hospitalised green turtles (Chelonia mydas) and their environment in North Queensland, Australia, for in vitro susceptibility to bacteriophages. Seventy-one Gram-negative bacteria were isolated from green turtle eye swabs and water samples. Broth microdilution tests were used to determine antibiotic susceptibility. All isolates were resistant to at least two antibiotics, with 24% being resistant to seven of the eight antibiotics. Highest resistance rates were detected to enrofloxacin (77%) and ampicillin (69...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29129977/preinoculation-of-soybean-seeds-treated-with-agrichemicals-up-to-30-days-before-sowing-technological-innovation-for-large-scale-agriculture
#20
Ricardo Silva Araujo, Sonia Purin da Cruz, Edson Luiz Souchie, Thomas Newton Martin, André Shigueyoshi Nakatani, Marco Antonio Nogueira, Mariangela Hungria
The cultivation of soybean in Brazil experienced an expressive growth in the last decades. Soybean is highly demanding on nitrogen (N) that must come from fertilizers or from biological fixation. The N supply to the soybean crop in Brazil relies on the inoculation with elite strains of Bradyrhizobium japonicum, B. elkanii, and B. diazoefficiens , which are able to fulfill the crop's N requirements and enrich the soil for the following crop. The effectiveness of the association between N2 -fixing bacteria and soybean plants depends on the efficacy of the inoculation process...
2017: International Journal of Microbiology
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