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International Journal of Microbiology

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https://www.readbyqxmd.com/read/29259629/qualitative-analysis-of-microbial-dynamics-during-anaerobic-digestion-of-microalgal-biomass-in-a-uasb-reactor
#1
Anna Doloman, Yousef Soboh, Andrew J Walters, Ronald C Sims, Charles D Miller
Anaerobic digestion (AD) is a microbiologically coordinated process with dynamic relationships between bacterial players. Current understanding of dynamic changes in the bacterial composition during the AD process is incomplete. The objective of this research was to assess changes in bacterial community composition that coordinates with anaerobic codigestion of microalgal biomass cultivated on municipal wastewater. An upflow anaerobic sludge blanket reactor was used to achieve high rates of microalgae decomposition and biogas production...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29234354/differential-microbial-diversity-in-drosophila-melanogaster-are-fruit-flies-potential-vectors-of-opportunistic-pathogens
#2
Luis A Ramírez-Camejo, Génesis Maldonado-Morales, Paul Bayman
Drosophila melanogaster has become a model system to study interactions between innate immunity and microbial pathogens, yet many aspects regarding its microbial community and interactions with pathogens remain unclear. In this study wild D. melanogaster were collected from tropical fruits in Puerto Rico to test how the microbiota is distributed and to compare the culturable diversity of fungi and bacteria. Additionally, we investigated whether flies are potential vectors of human and plant pathogens. Eighteen species of fungi and twelve species of bacteria were isolated from wild flies...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29234353/enteric-fever-caused-by-salmonella-enterica-serovars-with-reduced-susceptibility-of-fluoroquinolones-at-a-community-based-teaching-hospital-of-nepal
#3
Anjeela Bhetwal, Anjila Maharjan, Puspa Raj Khanal, Narayan Prasad Parajuli
Enteric fever continues to be an important public health problem especially in developing countries of the tropical region including Nepal. In this study, we aimed to investigate the incidence of enteric fever associated with Salmonella enterica and determine its antimicrobial susceptibilities to therapeutic antimicrobials in a community based teaching hospital of Nepal. A total of 2,304 blood samples from suspected enteric fever patients attending Manmohan Memorial Teaching Hospital were processed with standard microbiological methods for the isolation and identification of bacterial pathogens...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29213286/survival-of-five-strains-of-shiga-toxigenic-escherichia-coli-in-a-sausage-fermentation-model-and-subsequent-sensitivity-to-stress-from-gastric-acid-and-intestinal-fluid
#4
Tone Mari Rode, Anette McLeod, Ingrid Måge, Even Heir, Lars Axelsson, Askild L Holck
The ability of foodborne pathogens to exhibit adaptive responses to stressful conditions in foods may enhance their survival when passing through the gastrointestinal system. We aimed to determine whether Escherichia coli surviving stresses encountered during a model dry-fermented sausage (DFS) production process exhibit enhanced tolerance and survival in an in vitro gastrointestinal model. Salami sausage batters spiked with five E. coli isolates, including enterohaemorrhagic E. coli strains isolated from different DFS outbreaks, were fermented in a model DFS process (20°C, 21 days)...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29181028/comparison-of-the-performance-of-urinary-mycobacterium-tuberculosis-antigens-cocktail-esat6-cfp10-and-mpt64-with-culture-and-microscopy-in-pulmonary-tuberculosis-patients
#5
Dewi Kartika Turbawaty, Adhi Kristianto Sugianli, Arto Yuwono Soeroto, Budi Setiabudiawan, Ida Parwati
Pulmonary tuberculosis (TB) is a major global health problem and is one of the top 10 causes of death worldwide. Our study aimed to evaluate the performance of urinary Mycobacterium tuberculosis (Mtb) antigens cocktail (ESAT6, CFP10, and MPT64) compared with culture and microscopy. This descriptive cross-sectional study was conducted in Dr. Hasan Sadikin General Hospital, Bandung, from January 2014 to October 2016. A total of 141 pulmonary tuberculosis patients were included. Sputum samples were examined for acid-fast bacilli (ZN stain) and mycobacterial culture (LJ); the Mtb antigens cocktail was examined in the urine sample...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29163641/isolation-and-characterization-of-thermophilic-bacteria-from-jordanian-hot-springs-bacillus-licheniformis-and-thermomonas-hydrothermalis-isolates-as-potential-producers-of-thermostable-enzymes
#6
Balsam T Mohammad, Hala I Al Daghistani, Atef Jaouani, Saleh Abdel-Latif, Christian Kennes
The aim of this study was the isolation and characterization of thermophilic bacteria from hot springs in Jordan. Ten isolates were characterized by morphological, microscopic, biochemical, molecular, and physiological characteristics. Sequencing of the 16S rDNA of the isolates followed by BLAST search revealed that nine strains could be identified as Bacillus licheniformis and one isolate as Thermomonas hydrothermalis. This is the first report on the isolation of Thermomonas species from Jordanian hot springs...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29147114/antibiotic-resistant-bacterial-isolates-from-captive-green-turtles-and-in-vitro-sensitivity-to-bacteriophages
#7
Alessandro Delli Paoli Carini, Ellen Ariel, Jacqueline Picard, Lisa Elliott
This study aimed to test multidrug resistant isolates from hospitalised green turtles (Chelonia mydas) and their environment in North Queensland, Australia, for in vitro susceptibility to bacteriophages. Seventy-one Gram-negative bacteria were isolated from green turtle eye swabs and water samples. Broth microdilution tests were used to determine antibiotic susceptibility. All isolates were resistant to at least two antibiotics, with 24% being resistant to seven of the eight antibiotics. Highest resistance rates were detected to enrofloxacin (77%) and ampicillin (69...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29129977/preinoculation-of-soybean-seeds-treated-with-agrichemicals-up-to-30-days-before-sowing-technological-innovation-for-large-scale-agriculture
#8
Ricardo Silva Araujo, Sonia Purin da Cruz, Edson Luiz Souchie, Thomas Newton Martin, André Shigueyoshi Nakatani, Marco Antonio Nogueira, Mariangela Hungria
The cultivation of soybean in Brazil experienced an expressive growth in the last decades. Soybean is highly demanding on nitrogen (N) that must come from fertilizers or from biological fixation. The N supply to the soybean crop in Brazil relies on the inoculation with elite strains of Bradyrhizobium japonicum, B. elkanii, and B. diazoefficiens, which are able to fulfill the crop's N requirements and enrich the soil for the following crop. The effectiveness of the association between N2-fixing bacteria and soybean plants depends on the efficacy of the inoculation process...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29123549/isolation-and-physiomorphological-characterization-of-escherichia-coli-o157-h7-infecting-bacteriophages-recovered-from-beef-cattle-operations
#9
Pushpinder Kaur Litt, Divya Jaroni
Bacteriophages, recovered from beef cattle environment and specifically targeting Escherichia coli O157:H7, were examined for their physiological and morphological characteristics. Degree of bacterial lysis and host range of isolated bacteriophages was determined against 55 isolates of E. coli O157:H7. Morphology of phages was examined under transmission electron microscope. Phage growth parameters, particularly rate of adsorption, rise period, latent period, and burst size were also determined. The stability of isolated phages was tested at acidic and alkaline pH, at high temperatures, and in cold storage...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29098004/predicted-cold-shock-proteins-from-the-extremophilic-bacterium-deinococcus-maricopensis-and-related-deinococcus-species
#10
Michael J LaGier
While many studies have examined the mechanisms by which extremophilic Deinococci survive exposure to ionizing radiation, very few publications have characterized the cold shock adaptations of this group, despite many species being found in persistent cold environments and environments prone to significant daily temperature fluctuations. Bacterial cold shock proteins (Csps) are a family of conserved, RNA chaperone proteins that commonly play a role in cold temperature adaptation, including a downward shift in temperature (i...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29090008/automated-flow-cytometry-an-alternative-to-urine-culture-in-a-routine-clinical-microbiology-laboratory
#11
REVIEW
Patricia Mejuto, Mariam Luengo, Julio Díaz-Gigante
The urine culture is the "gold standard" for the diagnosis of urinary tract infections (UTI) but constitutes a significant workload in the routine clinical laboratory. Due to the high percentage of negative results, there is a need for an efficient screening method, with a high negative predictive value (NPV) that could reduce the number of unnecessary culture tests. With the purpose of improving the efficiency of laboratory work, several methods for screening out the culture-negative samples have been developed, but none of them has shown adequate sensitivity (SE) and high NPV...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29090007/corrigendum-to-spread-of-tem-vim-shv-and-ctx-m-%C3%AE-lactamases-in-imipenem-resistant-gram-negative-bacilli-isolated-from-egyptian-hospitals
#12
El Sayed Hamdy Mohammed, Ahmed Elsadek Fakhr, Hanan Mohammed El Sayed, Said Abd Elmohsen Al Johery, Wesam Abdel Ghani Hassanein, Ahmed Mansour Alzohairy
[This corrects the article DOI: 10.1155/2016/8382605.].
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29081804/baseline-antibody-titre-against-salmonella-enterica-in-healthy-population-of-mumbai-maharashtra-india
#13
Rucha Patki, Sunil Lilani, Dhaneshwar Lanjewar
OBJECTIVE: The aim of this study was to establish a baseline titre for the population of Mumbai, Maharashtra, India. METHOD: Four hundred healthy blood donors, attending blood donation camps, were screened using a survey questionnaire. Widal tube agglutination test was performed on the diluted sera (with 0.9% normal saline) of blood donors, with final dilution ranging from 1 : 40 to 1 : 320. RESULTS: Out of 400 individuals providing samples, 78 (19...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29081803/environmentally-friendly-production-of-d-lactic-acid-by-sporolactobacillus-nakayamae-investigation-of-fermentation-parameters-and-fed-batch-strategies
#14
Susan Michelz Beitel, Luciana Fontes Coelho, Daiane Cristina Sass, Jonas Contiero
The interest in the production of lactic acid has increased due to its wide range of applications. In the present study, the variables that affect fermentative D(-) lactic acid production were investigated: neutralizing agents, pH, temperature, inoculum percentage, agitation, and concentration of the medium components. An experimental design was applied to determine the optimal concentrations of the medium components and fermentation was studied using different feeding strategies. High production (122.41 g/L) and productivity (3...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29056969/clinical-characteristics-and-laboratory-identification-of-aerococcus-infections-an-australian-tertiary-centre-perspective
#15
Shanti Narayanasamy, Katherine King, Amanda Dennison, Denis W Spelman, Ar Kar Aung
Aerococci uncommonly cause urinary tract (UTI) and bloodstream infections (BSI). The clinical characteristics and laboratory identification rates of Aerococcus in the Australian context are unknown. A retrospective observational cohort study of patients with positive Aerococcus cultures between 2010 and 2015 was performed. Patients were analysed according to predefined "asymptomatic bacteriuria," "UTI," and "BSI" groups. Forty-seven [40 (85%) for urine and 7 (15%) for blood] isolates were identified [38% male, median age of 79 (IQR 62-85) years], with corresponding identification rates of 24...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29038662/multidrug-resistant-enteric-bacterial-pathogens-in-a-psychiatric-hospital-in-ghana-implications-for-control-of-nosocomial-infections
#16
Kwabena O Duedu, George Offei, Francis S Codjoe, Eric S Donkor
Enteric bacteria are commonly implicated in hospital-acquired or nosocomial infections. In Ghana, these infections constitute an important public health problem but little is known about their contribution to antibiotic resistance. The aim of the study was to determine the extent and pattern of antibiotic resistance of enteric bacteria isolated from patients and environmental sources at the Accra Psychiatric Hospital. A total of 265 samples were collected from the study site including 142 stool and 82 urine samples from patients, 7 swab samples of door handle, and 3 samples of drinking water...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28912815/feasibility-of-metatranscriptome-analysis-from-infant-gut-microbiota-adaptation-to-solid-foods-results-in-increased-activity-of-firmicutes-at-six-months
#17
Floor Hugenholtz, Jarmo Ritari, Lotta Nylund, Mark Davids, Reetta Satokari, Willem M de Vos
Newborns are rapidly colonized by microbes and their intestinal tracts contain highly dynamic and rapidly developing microbial communities in the first months of life. In this study, we describe the feasibility of isolating mRNA from rapidly processed faecal samples and applying deep RNA-Seq analysis to provide insight into the active contributors of the microbial community in early life. Specific attention is given to the impact of removing rRNA from the mRNA on the phylogenetic and transcriptional profiling and its analysis depth...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28831285/prevalence-of-bacterial-vaginosis-and-associated-risk-factors-among-women-complaining-of-genital-tract-infection
#18
Adane Bitew, Yeshiwork Abebaw, Delayehu Bekele, Amete Mihret
BACKGROUND: Bacterial vaginosis is a global concern due to the increased risk of acquisition of sexually transmitted infections. OBJECTIVES: To determine the prevalence of bacterial vaginosis and bacteria causing aerobic vaginitis. METHODS: A cross-sectional study was conducted among 210 patients between September 2015 and July 2016 at St. Paul's Hospital. Gram-stained vaginal swabs were examined microscopically and graded as per Nugent's procedure...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28814955/characterization-of-carbapenem-resistant-acinetobacter-baumannii-strains-isolated-from-hospitalized-patients-in-palestine
#19
Regeen Handal, Lulu Qunibi, Ibrahim Sahouri, Maha Juhari, Rula Dawodi, Hiyam Marzouqa, Musa Hindiyeh
The American Centers for Disease Control and Prevention (CDC) recognizes Acinetobacter baumannii as a source of global outbreaks and epidemics especially due to its increasing resistance to commercially available antibiotics. In this study, 69 single patient multidrug resistant isolates collected from all over Palestine, except Gaza, were studied. All the isolates were resistant to all the β-lactam antibiotics including the carbapenems. Of the 69 isolates, 82.6% were positive for blaOXA-23, 14.5% were positive for blaOXA-24, and 3% were positive for blaOXA-58...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/28757872/production-characterization-and-antimicrobial-activity-of-mycocin-produced-by-debaryomyces-hansenii-dsmz70238
#20
Safaa A S Al-Qaysi, Halah Al-Haideri, Zaid Akram Thabit, Wijdan Hameed Abd Al-Razzaq Al-Kubaisy, Jamal Abd Al-Rahman Ibrahim
The present study was conducted to estimate the antimicrobial activity and the potential biological control of the killer toxin produced by D. hansenii DSMZ70238 against several pathogenic microorganisms. In this study, the effects of NaCl, pH, and temperature, killer toxin production, and antimicrobial activity were studied. The results showed that the optimum inhibitory effect of killer toxin was at 8% NaCl, and the diameters of clear zones were 20, 22, 22, 21, 14, and 13 mm for Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Candida albicans, and Candida neoformans, respectively...
2017: International Journal of Microbiology
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