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Virologica Sinica

Zheng Fang, Jingxu Shao, Qingbei Weng
Cells of the P8-Se301-C1 strain are Spodoptera exigua cell clones that each harbor a partial version of the S. exigua multiple nucleopolyhedrovirus (SeMNPV) genome and which are resistant to homologous SeMNPV infections. The cells produce no viral progeny, suggesting that the infection is a latent-like viral infection. To investigate the SeMNPV genes harbored in the P8-Se301-C1 cells, the de novo transcriptomes of P8-Se301-C1 cells and S. exigua Se301 cells were analyzed and compared. A total of 54,569,296 reads were obtained from the P8-Se301-C1 cells that yielded 112,565 final unigenes with a mean length of 1,093 nt...
October 18, 2016: Virologica Sinica
Xiaowei Zhang, Fei Zhang, Xiaohe Ma, Xing Zhao, Wei Li, Zhiping Zhang, Jibin Zhang, Xian-En Zhang, Zongqiang Cui
Human immunodeficiency virus-1 (HIV-1) encodes 15 viral proteins. Protein-protein interactions play a large role in the function of these proteins. In this study, we attempted to identify novel interactions between the HIV-1 proteins to better understand the role played by viral protein-protein interactions in the life cycle of HIV-1. Genes encoding the 15 viral proteins from the HIV-1 strain AD8 were inserted into the plasmids of a yeast two-hybrid system. By screening 120 pairs of proteins, interactions between seven pairs were found...
September 26, 2016: Virologica Sinica
Hailiang Sun, Jian-Li Xue, Elizabeth Bailey, Yifei Xu, Guoliang Hu, John Baroch, Yi Zhang, Lanny Pace, Thomas J DeLiberto, Xiu-Feng Wan
No abstract text is available yet for this article.
September 18, 2016: Virologica Sinica
Fang Wei, Qing Zhu, Ling Ding, Qing Liang, Qiliang Cai
The cell membrane regulates many physiological processes including cellular communication, homing and metabolism. It is therefore not surprising that the composition of the host cell membrane is manipulated by intracellular pathogens. Among these, the human oncogenic herpesviruses Epstein-Barr virus (EBV) and Kaposi's sarcoma-associated herpesvirus (KSHV) exploit the host cell membrane to avoid immune surveillance and promote viral replication. Accumulating evidence has shown that both EBV and KSHV directly encode several similar membrane-associated proteins, including receptors and receptor-specific ligands (cytokines and chemokines), to increase virus fitness in spite of host antiviral immune responses...
September 12, 2016: Virologica Sinica
Fengling Luo, Tielong Chen, Jun Liu, Xihui Shen, Yinnan Zhao, Rongge Yang, Xiaolian Zhang
Ficolin-2 is a lectin complement pathway activator present in normal human plasma and usually associated with infectious diseases, but little is known about the role of ficolin-2 in human immunodeficiency virus (HIV) infection. Here, we describe our novel findings that serum ficolin-2 concentrations of 103 HIV-1 patients were much higher compared to those of 57 healthy donors. In vitro analysis showed that HIV-1 infection could enhance ficolin-2 expression. We further demonstrated that recombinant ficolin-2 protein could bind with HIV-1 envelope glycoprotein gp120, and subsequently induce complement dependent cytotoxicity...
August 29, 2016: Virologica Sinica
Yousong Peng, Dayan Wang, Yuelong Shu, Taijiao Jiang
No abstract text is available yet for this article.
August 9, 2016: Virologica Sinica
Wei Wang, Zheng Zhou, Leike Zhang, Shaobo Wang, Gengfu Xiao
Mammarenaviruses, including lethal pathogens such as Lassa virus and Junín virus, can cause severe hemorrhagic fever in humans. Entry is a key step for virus infection, which starts with binding of the envelope glycoprotein (GP) to receptors on target cells and subsequent fusion of the virus with target cell membranes. The GP precursor is synthesized as a polypeptide, and maturation occurs by two cleavage events, yielding a tripartite GP complex (GPC) formed by a stable signal peptide (SSP), GP1 and GP2. The unique retained SSP interacts with GP2 and plays essential roles in virion maturation and infectivity...
August 4, 2016: Virologica Sinica
Can Zhang, Yanxiang Ma, Ting Wang, Huzhi Sun, Guomin Lu, Huiying Ren
No abstract text is available yet for this article.
August 2016: Virologica Sinica
Yuting Qiao, Zhiwei Sui, Guoliang Hu, Huabin Cao, Guoxiang Yang, Yong Li, Yongsong Lei, Lihua Zhao, Quanjiao Chen
Hepatitis A virus is a pathogen associated with water pollution. Contaminated drinking water can cause hepatitis A outbreaks, lead to economic losses, and even threaten human lives. It is difficult to detect low levels of hepatitis A virus in water, so the virus must be concentrated in order to quantify it accurately. Here, we present a simple, rapid, efficient technique for the concentration and detection of hepatitis A virus in water. Our data showed that adding phosphate-buffered saline to the water, pre-filtering the water, and adding Trizol reagent directly to the filtration membrane can significantly improve concentration efficiency...
August 2016: Virologica Sinica
Xiaojing Kuang, Lingling Sun, Shuzhen Liu, Zhenzhen Zhao, Danrui Zhao, Song Liu, Bing Luo
The relationship between the Filaggrin gene (FLG) rs2065955 polymorphism and susceptibility to Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) and EBV-negative gastric carcinoma (EBVnGC) was investigated in Shandong Province, China. We detected the FLG rs2065955 genotype and allele distribution by using PCR and restriction fragment length polymorphism (RFLP) in 64 EBVaGC, 82 EBVnGC, and 111 normal control samples. Immunohistochemistry was used to detect the level of FLG protein in 35 EBVaGC and 51 EBVnGC tumor tissues...
August 2016: Virologica Sinica
Hedi Zhou, Bryan Eaton, Zhihong Hu, Basil Arif
No abstract text is available yet for this article.
August 2016: Virologica Sinica
Yuhai Bi, Jianjun Chen, Zhenjie Zhang, Mingxin Li, Tianlong Cai, Kirill Sharshov, Ivan Susloparov, Alexander Shestopalov, Gary Wong, Yubang He, Zhi Xing, Jianqing Sun, Di Liu, Yingxia Liu, Lei Liu, Wenjun Liu, Fumin Lei, Weifeng Shi, George F Gao
A novel Clade H5N1 reassortant virus caused several outbreaks in wild birds in some regions of China from late 2014 to 2015. Based on the genetic and phylogenetic analyses, the viruses possess a stable gene constellation with a Clade HA, a H9N2-derived PB2 gene and the other six genes of Asian H5N1-origin. The Clade H5N1 reassortants displayed a high genetic relationship to a human H5N1 strain (A/Alberta/01/2014). Further analysis showed that similar viruses have been circulating in wild birds in China, Russia, Dubai (Western Asia), Bulgaria and Romania (Europe), as well as domestic poultry in some regions of Africa...
August 2016: Virologica Sinica
Zhen Liu, Zhe Chen, Jian Hong, Xuefeng Wang, Changyong Zhou, Xueping Zhou, Jianxiang Wu
Citrus tristeza virus (CTV) is one of the most economically important citrus viruses and harms the citrus industry worldwide. To develop reliable and effective serological detection assays of CTV, the major capsid protein (CP) gene of CTV was expressed in Escherichia coli BL21 (DE3) using the expression vector pET-28a and purified through Ni+-NTA affinity chromatography. The recombinant protein was used to immunize BALB/c mice. Four hybridoma cell lines (14B10, 14H11, 20D5, and 20G12) secreting monoclonal antibodies (MAbs) against CTV were obtained through conventional hybridoma technology...
August 2016: Virologica Sinica
Jinliang Liu, Shiyu Dai, Manli Wang, Zhihong Hu, Hualin Wang, Fei Deng
Emerging infectious diseases are major threats to human health. Most severe viral disease outbreaks occur in developing regions where health conditions are poor. With increased international travel and business, the possibility of eventually transmitting infectious viruses between different countries is increasing. The most effective approach in preventing viral diseases is vaccination. However, vaccines are not currently available for numerous viral diseases. Virus-like particles (VLPs) are engineered vaccine candidates that have been studied for decades...
August 2016: Virologica Sinica
Qingxiu Chen, Jie Zhang, Fuxian Zhang, Hong Guo, Qin Fang
Aquareovirus species vary with respect to pathogenicity, and the nonstructural protein NS80 of aquareoviruses has been implicated in the regulation of viral replication and assembly, which can form viral inclusion bodies (VIBs) and recruit viral proteins to its VIBs in infected cells. NS80 consists of 742 amino acids with a molecular weight of approximately 80 kDa. Interestingly, a short specific fragment of NS80 has also been detected in infected cells. In this study, an approximately 58-kDa product of NS80 was confirmed in various infected and transfected cells by immunoblotting analyses using α-NS80C...
August 2016: Virologica Sinica
Jian Lei, Rolf Hilgenfeld
The papain-like protease (PL(pro)) of Middle-East respiratory syndrome coronavirus (MERS-CoV) has proteolytic, deubiquitinating, and deISGylating activities. The latter two are involved in the suppression of the antiviral innate immune response of the host cell. To contribute to an understanding of this process, we present here the X-ray crystal structure of a complex between MERS-CoV PL(pro) and human ubiquitin (Ub) that is devoid of any covalent linkage between the two proteins. Five regions of the PL(pro) bind to two areas of the Ub...
August 2016: Virologica Sinica
Xin Huang, Xuan Zhou, Xiaoyan He, Pei Wang, Shuai Yue, Lixin Wu, Yu Zhang, Qian Xie, Bao Zhang, Wei Zhao
No abstract text is available yet for this article.
August 2016: Virologica Sinica
Wenhua Kuang, Huanyu Zhang, Dianhai Hou, Manli Wang, Fei Deng, Hualin Wang, Zhihong Hu
No abstract text is available yet for this article.
August 2016: Virologica Sinica
Hui Ding, Wuritu Yang, Hua Tang, Peng-Mian Feng, Jian Huang, Wei Chen, Hao Lin
No abstract text is available yet for this article.
August 2016: Virologica Sinica
Xiaoli Wang, Chaoyun Shen, Tan Chen, Ke Lan, Zhong Huang, Yunfang Zhang, Qingwei Liu
No abstract text is available yet for this article.
August 2016: Virologica Sinica
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