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Virologica Sinica

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https://www.readbyqxmd.com/read/29987490/molecular-epidemiology-of-porcine-circovirus-type-3-infection-in-swine-herds-in-china
#1
LETTER
Xiangdong Li, Chaolin Zhang, Mingming Qiao, Jiangsong Guo, Guangyuan Xing, Chunxia Jin, Juan Wang, Ming Sun, Kegong Tian
No abstract text is available yet for this article.
July 9, 2018: Virologica Sinica
https://www.readbyqxmd.com/read/30155853/genome-characteristics-of-the-cyclophragma-undans-nucleopolyhedrovirus-a-distinct-species-in-group-i-of-alphabaculovirus
#2
Zheng Zhu, Jun Wang, Qianran Wang, Feifei Yin, Xiaoping Liu, Dianhai Hou, Lei Zhang, Haizhou Liu, Jiang Li, Basil M Arif, Hualin Wang, Fei Deng, Zhihong Hu, Manli Wang
The Cyclophragma undans nucleopolyhedrovirus (CyunNPV), a potential pest control agent, was isolated from Cyclophragma undans (Lepidoptera: Lasiocampidae), an important forest pest. In the present study, we performed detailed genome analysis of CyunNPV and compared its genome to those of other Group I alphabaculoviruses. Sequencing of the CyunNPV genome using the Roche 454 sequencing system generated 142,900 bp with a G + C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group I Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to CyunNPV...
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/30155852/serological-cross-reactivity-between-zika-and-dengue-viruses-in-experimentally-infected-monkeys
#3
LETTER
Shailendra Mani, Chee Wah Tan, Lin-Fa Wang, Danielle E Anderson
No abstract text is available yet for this article.
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/30069823/tandem-3-utr-patterns-and-gene-expression-profiles-of-marc-145-cells-during-prrsv-infection
#4
Ying Wei, Jie Li, Yun Zhang, Chunyi Xue, Yongchang Cao
Porcine reproductive and respiratory syndrome virus (PRRSV) causes substantial economic losses to the global pig industry. Alternative polyadenylation (APA) is a mechanism that diversifies gene expression, which is important for tumorigenesis, development, and cell differentiation. However, it is unclear whether APA plays a role in the course of PRRSV infection. To address this issue, in this study we carried out a whole-genome transcriptome analysis of PRRSV-infected Marc-145 African green monkey kidney cells and identified 185 APA switching genes and 393 differentially expressed genes (DEGs)...
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/30066045/serological-investigation-of-laboratory-confirmed-and-suspected-ebola-virus-disease-patients-during-the-late-phase-of-the-ebola-outbreak-in-sierra-leone
#5
Yang Liu, YuLan Sun, Wei Wu, AQian Li, XianDa Yang, Shuo Zhang, Chuan Li, QiuDong Su, ShaoJian Cai, DaPeng Sun, HaiYang Hu, Zhe Zhang, XiuXu Yang, Idrissa Kamara, Sheku Koroma, Gerald Bangura, Alie Tia, Abdul Kamara, Matt Lebby, Brima Kargbo, Jiandong Li, Shiwen Wang, XiaoPing Dong, YueLong Shu, WenBo Xu, George F Gao, GuiZhen Wu, DeXin Li, William J Liu, MiFang Liang
This study aimed to investigate the serological characteristics of Ebola virus (EBOV) infection during the late phase of the Ebola outbreak in Sierra Leone. In total, 877 blood samples from 694 suspected Ebola virus disease (EVD) cases assessed from March to December 2015, were analyzed via real-time reverse transcription polymerase chain reaction (RT-PCR) for viral RNA and enzyme-linked immunosorbent assay (ELISA) and Luminex to detect antibodies against EBOV. Viral load and EBOV-specific IgM/IgG titers displayed a declining trend during March to December 2015...
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/30062589/molecular-genetic-characterization-of-human-rotavirus-a-strains-circulating-in-moscow-russia-2009-2014
#6
Victoria Kiseleva, Evgeny Faizuloev, Elena Meskina, Anna Marova, Alexey Oksanich, Tatiana Samartseva, Georgy Bakhtoyarov, Natalia Bochkareva, Nikolay Filatov, Andrey Linok, Yulia Ammour, Vitaly Zverev
Enteric viruses are the most common cause of acute gastroenteritis (AGE) in young children and a significant public health problem globally. Hospital admissions of children under 5 years of age with diarrhea are primarily associated with group A rotavirus (RVA) infection. In this retrospective study, the population structure of viruses linked to AGE etiology in young children hospitalized with AGE in Moscow was evaluated, and molecular characterization of RVA strains was performed. Fecal specimens were collected from children under 5 years old hospitalized with AGE between 2009 and 2014 in Moscow, Russia...
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/30046995/insights-into-the-temporal-gene-expression-pattern-in-lymantria-dispar-larvae-during-the-baculovirus-induced-hyperactive-stage
#7
Upendra Raj Bhattarai, Mandira Katuwal Bhattarai, Fengjiao Li, Dun Wang
Baculoviruses are effective biological control agents for many insect pests. They not only efficiently challenge the host immune system but also make them hyperactive for better virus dispersal. Some investigations have focused on the viral mechanisms for induction of such altered response from the host. However, there are no current studies monitoring changes in gene expression during this altered phenotype in infected larvae. The L. dispar multiple nucleopolyhedrovirus (LdMNPV) induces hyperactivity in third instar L...
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/30039498/kir2dl2-c1-is-a-risk-factor-for-chronic-infection-and-associated-with-non-response-to-peg-ifn-and-rbv-combination-therapy-in-hepatitis-c-virus-genotype-1b-patients-in-china
#8
LETTER
Song Hu, Fahu Yuan, Lingyan Feng, Fang Zheng, Feili Gong, Hanju Huang, Binlian Sun
No abstract text is available yet for this article.
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29959686/stress-granule-formation-is-one-of-the-early-antiviral-mechanisms-for-host-cells-against-coxsackievirus-b-infection
#9
Xia Zhai, Shuo Wu, Lexun Lin, Tianying Wang, Xiaoyan Zhong, Yang Chen, Weizhen Xu, Lei Tong, Yan Wang, Wenran Zhao, Zhaohua Zhong
Stress granules (SGs) are intracellular granules formed when cellular translation is blocked and have been reported to be involved in a variety of viral infections. Our previous studies revealed that SGs are involved in the coxsackievirus B (CVB) infection process, but the role of SGs in CVB infection has not been fully explored. In this study, we found that CVB type 3 (CVB3) could induce SG formation in the early phase of infection. Results showed that levels of CVB3 RNA and protein were significantly inhibited during the early stage of CVB3 infection by the elevated formation of SGs, while viral RNA and protein synthesis were significantly promoted when SG formation was blocked...
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29948850/hepatitis-c-in-laos-a-7-year-retrospective-study-on-1765-patients
#10
Phimpha Paboriboune, Thomas Vial, Philavanh Sitbounlang, Stéphane Bertani, Christian Trépo, Paul Dény, Francois-Xavier Babin, Nicolas Steenkeste, Pascal Pineau, Eric Deharo
Hepatitis C virus (HCV) is a global health concern, notably in Southeast Asia, and in Laos the presentation of the HCV-induced liver disease is poorly known. Our objective was thus to describe a comprehensive HCV infection pattern in order to guide national health policies. A study on a group of 1765 patients formerly diagnosed by rapid test in health centres was conducted at the Centre of Infectiology Lao Christophe Merieux in Vientiane. The demographic information of patients, their infection status (viral load: VL), liver function (aminotransferases) and treatments were analysed...
August 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29931514/micro-droplet-digital-polymerase-chain-reaction-and-real-time-quantitative-polymerase-chain-reaction-technologies-provide-highly-sensitive-and-accurate-detection-of-zika-virus
#11
Yuan Hui, Zhiming Wu, Zhiran Qin, Li Zhu, Junhe Liang, Xujuan Li, Hanmin Fu, Shiyu Feng, Jianhai Yu, Xiaoen He, Weizhi Lu, Weiwei Xiao, Qinghua Wu, Bao Zhang, Wei Zhao
The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus (ZIKV) and in preventing serious neurological complications of ZIKV infection. In this study, we established micro-droplet digital polymerase chain reaction (ddPCR) and real-time quantitative PCR (RT-qPCR) protocols for the detection of ZIKV based on the amplification of the NS5 gene. For the ZIKV standard plasmid, the RT-qPCR results showed that the cycle threshold (Ct) value was linear from 101 to 108  copy/μL, with a standard curve R2 of 0...
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29931513/non-chinese-immigrants-challenge-faced-by-yunnan-of-china-to-achieve-the-90-90-90-goals
#12
Xin Chen, Lin Duo, Mei Ye, Chiyu Zhang, Yong-Tang Zheng
No abstract text is available yet for this article.
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29869748/n-terminal-myristoylated-vp5-is-required-for-penetrating-cell-membrane-and-promoting-infectivity-in-aquareoviruses
#13
LETTER
Qingxiu Chen, Hong Guo, Fuxian Zhang, Qin Fang
No abstract text is available yet for this article.
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29808439/correction-to-molecular-detection-of-viruses-in-kenyan-bats-and-discovery-of-novel-astroviruses-caliciviruses-and-rotaviruses
#14
Cecilia Waruhiu, Sheila Ommeh, Vincent Obanda, Bernard Agwanda, Francis Gakuya, Xing-Yi Ge, Xing-Lou Yang, Li-Jun Wu, Ali Zohaib, Ben Hu, Zheng-Li Shi
The affiliation listed for Cecilia Waruhiu is incorrect. The byline and affiliation line should appear as shown above.
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29797225/emergence-of-eurasian-avian-like-swine-influenza-a-h1n1-virus-from-an-adult-case-in-fujian-province-china
#15
LETTER
Jian-Feng Xie, Yan-Hua Zhang, Lin Zhao, Wen-Qiong Xiu, Hong-Bin Chen, Qi Lin, Yu-Wei Weng, Kui-Cheng Zheng
No abstract text is available yet for this article.
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29774519/zika-virus-baculovirus-expressed-virus-like-particles-induce-neutralizing-antibodies-in-mice
#16
Shiyu Dai, Tao Zhang, Yanfang Zhang, Hualin Wang, Fei Deng
The newly emerged mosquito-borne Zika virus (ZIKV) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders. Several ZIKV vaccine candidates have been proposed, including inactivated and live attenuated virus vaccines, vector-based vaccines, DNA and RNA vaccines. These have been shown to be efficacious in preclinical studies in mice and nonhuman primates, but their use will potentially be a threat to immunocompromised individuals and pregnant women. Virus-like particles (VLPs) are empty particles composed merely of viral proteins, which can serve as a safe and valuable tool for clinical prevention and treatment strategies...
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29737506/hiv-1-protein-tat-1-72-impairs-neuronal-dendrites-via-activation-of-pp1-and-regulation-of-the-creb-bdnf-pathway
#17
Yu Liu, Deyu Zhou, Jiabin Feng, Zhou Liu, Yue Hu, Chang Liu, Xiaohong Kong
Despite the success of combined antiretroviral therapy in recent years, the prevalence of human immunodeficiency virus (HIV)-associated neurocognitive disorders in people living with HIV-1 is increasing, significantly reducing the health-related quality of their lives. Although neurons cannot be infected by HIV-1, shed viral proteins such as transactivator of transcription (Tat) can cause dendritic damage. However, the detailed molecular mechanism of Tat-induced neuronal impairment remains unknown. In this study, we first showed that recombinant Tat (1-72 aa) induced neurotoxicity in primary cultured mouse neurons...
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29675605/histopathological-features-and-viral-antigen-distribution-in-the-lung-of-fatal-patients-with-enterovirus-71-infection
#18
LETTER
Zhenli Liang, Hongbo Pan, Xijing Wang, Yinchuan Zhu, Yiwu Dang, Xiaohui Fan, Lingxi Gao, Zengfeng Zhang
No abstract text is available yet for this article.
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29654554/evaluation-of-epstein-barr-virus-salivary-shedding-in-hiv-aids-patients-and-haart-use-a-retrospective-cohort-study
#19
Yan Yan, Yong Ren, Renfang Chen, Jing Hu, Yongjia Ji, Junyang Yang, Jiayin Shen, Lvyin Hu, Hao Pei, Jun Wang, Yuanwang Qiu, Hongzhou Lu, Lihua Huang
Little data is available on the evaluation of the occurrence rates of Epstein-Barr virus (EBV) in saliva and relationship with highly active antiretroviral therapy (HAART) use in HIV/AIDS patients in China. We conducted a retrospective cohort study of EBV serological tests for HIV/AIDS patients who were treated in the hospitals for infectious diseases in Wuxi and Shanghai, China from May 2016 to April 2017. The EBV-seropositive samples were identified by ELISA. EBV-specific primers and probes were used for the quantitative detection of viral DNA from saliva via quantitative real-time polymerase chain reaction...
June 2018: Virologica Sinica
https://www.readbyqxmd.com/read/29594957/exhaustive-exercise-does-not-affect-humoral-immunity-and-protection-after-rabies-vaccination-in-a-mouse-model
#20
Lun Xia, Minrui Li, Yajing Zhang, Juncheng Ruan, Jie Pei, Jiale Shi, Zhen F Fu, Ling Zhao, Ming Zhou, Dayong Tian
Rabies is one of the most dangerous and widespread zoonosis and is characterized by severe neurological signs and a high case-mortality rate of nearly 100%. Vaccination is the most effective way to prevent rabies in humans and animals. In this study, the relationship between exhaustive exercise and the humoral immune response after immunization with inactivated rabies vaccine was investigated in a mouse model with one-time exhaustive exercise. It was found that compared with the mice with no exercise after vaccination, no significant differences were found in those with exhaustive exercise after vaccination on body-weight changes, virus-neutralizing antibody (VNA) titers, antibody subtypes and survivor ratio after lethal rabies virus (RABV) challenge...
June 2018: Virologica Sinica
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