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Virologica Sinica

Xiangdong Li, Chaolin Zhang, Mingming Qiao, Jiangsong Guo, Guangyuan Xing, Chunxia Jin, Juan Wang, Ming Sun, Kegong Tian
No abstract text is available yet for this article.
July 9, 2018: Virologica Sinica
Xia Zhai, Shuo Wu, Lexun Lin, Tianying Wang, Xiaoyan Zhong, Yang Chen, Weizhen Xu, Lei Tong, Yan Wang, Wenran Zhao, Zhaohua Zhong
Stress granules (SGs) are intracellular granules formed when cellular translation is blocked and have been reported to be involved in a variety of viral infections. Our previous studies revealed that SGs are involved in the coxsackievirus B (CVB) infection process, but the role of SGs in CVB infection has not been fully explored. In this study, we found that CVB type 3 (CVB3) could induce SG formation in the early phase of infection. Results showed that levels of CVB3 RNA and protein were significantly inhibited during the early stage of CVB3 infection by the elevated formation of SGs, while viral RNA and protein synthesis were significantly promoted when SG formation was blocked...
June 29, 2018: Virologica Sinica
Phimpha Paboriboune, Thomas Vial, Philavanh Sitbounlang, Stéphane Bertani, Christian Trépo, Paul Dény, Francois-Xavier Babin, Nicolas Steenkeste, Pascal Pineau, Eric Deharo
Hepatitis C virus (HCV) is a global health concern, notably in Southeast Asia, and in Laos the presentation of the HCV-induced liver disease is poorly known. Our objective was thus to describe a comprehensive HCV infection pattern in order to guide national health policies. A study on a group of 1765 patients formerly diagnosed by rapid test in health centres was conducted at the Centre of Infectiology Lao Christophe Merieux in Vientiane. The demographic information of patients, their infection status (viral load: VL), liver function (aminotransferases) and treatments were analysed...
June 13, 2018: Virologica Sinica
Chun Xu, Mao Xia, Gang Meng, Chunyan Li, Aiqin Jiang, Jiwu Wei
Oncolytic measles virus (OMV) is a promising antitumor agent. However, the presence of anti-measles neutralizing antibodies (NAbs) against the hemagglutinin (H) protein of OMV is a major barrier to the therapeutic application of OMV in clinical practice. In order to overcome this challenge, specific types of cells have been used as carriers for OMV. Differential loading strategies appear to result in different therapeutic outcomes; despite this, only few studies have reported practical ex vivo loading strategies required for effective treatment...
May 16, 2018: Virologica Sinica
Peidian Shi, Lilin Zhang, Jiashun Wang, Dong Lu, Yi Li, Jie Ren, Menglu Shen, Lei Zhang, Jinhai Huang
Porcine reproductive and respiratory syndrome virus (PRRSV) shows characteristic antibody-dependent enhancement (ADE) of infection and causes porcine systemic inflammation, which is similar to a type I allergic reaction; however, the role of porcine FcεRI in ADE is still unclear. In this study, the expression of different Fc receptors (FcRs) on macrophages was investigated in a PRRSV 3D4/21 cell infection model in the presence or absence of PRRSV antibody. The transcription level of FcγII and FcεRI was significantly up-regulated under PRRSV-antibody complex infection...
May 14, 2018: Virologica Sinica
Yuan Hui, Zhiming Wu, Zhiran Qin, Li Zhu, Junhe Liang, Xujuan Li, Hanmin Fu, Shiyu Feng, Jianhai Yu, Xiaoen He, Weizhi Lu, Weiwei Xiao, Qinghua Wu, Bao Zhang, Wei Zhao
The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus (ZIKV) and in preventing serious neurological complications of ZIKV infection. In this study, we established micro-droplet digital polymerase chain reaction (ddPCR) and real-time quantitative PCR (RT-qPCR) protocols for the detection of ZIKV based on the amplification of the NS5 gene. For the ZIKV standard plasmid, the RT-qPCR results showed that the cycle threshold (Ct) value was linear from 101 to 108  copy/μL, with a standard curve R2 of 0...
June 2018: Virologica Sinica
Xin Chen, Lin Duo, Mei Ye, Chiyu Zhang, Yong-Tang Zheng
No abstract text is available yet for this article.
June 2018: Virologica Sinica
Qingxiu Chen, Hong Guo, Fuxian Zhang, Qin Fang
No abstract text is available yet for this article.
June 2018: Virologica Sinica
Cecilia Waruhiu, Sheila Ommeh, Vincent Obanda, Bernard Agwanda, Francis Gakuya, Xing-Yi Ge, Xing-Lou Yang, Li-Jun Wu, Ali Zohaib, Ben Hu, Zheng-Li Shi
The affiliation listed for Cecilia Waruhiu is incorrect. The byline and affiliation line should appear as shown above.
June 2018: Virologica Sinica
Jian-Feng Xie, Yan-Hua Zhang, Lin Zhao, Wen-Qiong Xiu, Hong-Bin Chen, Qi Lin, Yu-Wei Weng, Kui-Cheng Zheng
No abstract text is available yet for this article.
June 2018: Virologica Sinica
Shiyu Dai, Tao Zhang, Yanfang Zhang, Hualin Wang, Fei Deng
The newly emerged mosquito-borne Zika virus (ZIKV) strains pose a global challenge owing to its ability to cause microcephaly and neurological disorders. Several ZIKV vaccine candidates have been proposed, including inactivated and live attenuated virus vaccines, vector-based vaccines, DNA and RNA vaccines. These have been shown to be efficacious in preclinical studies in mice and nonhuman primates, but their use will potentially be a threat to immunocompromised individuals and pregnant women. Virus-like particles (VLPs) are empty particles composed merely of viral proteins, which can serve as a safe and valuable tool for clinical prevention and treatment strategies...
June 2018: Virologica Sinica
Yu Liu, Deyu Zhou, Jiabin Feng, Zhou Liu, Yue Hu, Chang Liu, Xiaohong Kong
Despite the success of combined antiretroviral therapy in recent years, the prevalence of human immunodeficiency virus (HIV)-associated neurocognitive disorders in people living with HIV-1 is increasing, significantly reducing the health-related quality of their lives. Although neurons cannot be infected by HIV-1, shed viral proteins such as transactivator of transcription (Tat) can cause dendritic damage. However, the detailed molecular mechanism of Tat-induced neuronal impairment remains unknown. In this study, we first showed that recombinant Tat (1-72 aa) induced neurotoxicity in primary cultured mouse neurons...
June 2018: Virologica Sinica
Zhenli Liang, Hongbo Pan, Xijing Wang, Yinchuan Zhu, Yiwu Dang, Xiaohui Fan, Lingxi Gao, Zengfeng Zhang
No abstract text is available yet for this article.
April 19, 2018: Virologica Sinica
Yan Yan, Yong Ren, Renfang Chen, Jing Hu, Yongjia Ji, Junyang Yang, Jiayin Shen, Lvyin Hu, Hao Pei, Jun Wang, Yuanwang Qiu, Hongzhou Lu, Lihua Huang
Little data is available on the evaluation of the occurrence rates of Epstein-Barr virus (EBV) in saliva and relationship with highly active antiretroviral therapy (HAART) use in HIV/AIDS patients in China. We conducted a retrospective cohort study of EBV serological tests for HIV/AIDS patients who were treated in the hospitals for infectious diseases in Wuxi and Shanghai, China from May 2016 to April 2017. The EBV-seropositive samples were identified by ELISA. EBV-specific primers and probes were used for the quantitative detection of viral DNA from saliva via quantitative real-time polymerase chain reaction...
April 13, 2018: Virologica Sinica
Minghao Zhang, Rui Chen, Xueping Zhou, Jianxiang Wu
Wheat dwarf disease caused by wheat dwarf virus (WDV) is currently present in wheat growing regions in China and causes serious losses in wheat yield. To develop reliable and effective serological detection methods for WDV, the coat protein (CP) gene of WDV was cloned and expressed in Escherichia coli. The purified recombinant CP protein was immunized to BALB/c mice, and four hybridoma cell lines (i.e. 18G10, 9G4, 23F4 and 22A10) secreting anti-WDV monoclonal antibodies (MAbs) were obtained through the hybridoma technique...
April 2018: Virologica Sinica
Xiangdong Li, Mingming Qiao, Ming Sun, Kegong Tian
Porcine circoviruses (PCV) include PCV1, PCV2, and the new-emerging PCV3. PCV2 is pathogenic to pigs, but the pathogenicity of PCV3 in pigs is debatable. Recently, there have been frequent reports of PCV2 and PCV3 co-infections in clinical samples. Thus, it would be practical to develop a duplex PCR method to detect PCV2 and PCV3 simultaneously. In this study, specific primers and probes were designed to target PCV2 cap and PCV3 rep genes. A duplex real-time PCR method was then developed to detect the two viruses...
April 2018: Virologica Sinica
Yilin Li, Yishuang Sun, Fuyun Sun, Rong Hua, Chenlin Li, Lang Chen, Deyin Guo, Jingfang Mu
Hepatitis B virus (HBV) infection is one of the major problems that threatens global health. There have been many studies on HBV, but the relationship between HBV and host factors is largely unexplored and more studies are needed to clarify these interactions. Filamin B is an actin-binding protein that acts as a cytoskeleton protein, and it is involved in cell development and several signaling pathways. In this study, we showed that filamin B interacted with HBV core protein, and the interaction promoted HBV replication...
April 2018: Virologica Sinica
Hewei Zhang, Mingqi Xia, Wei Wang, Decai Ju, Long Cao, Bai Wu, Xin Wang, Ying Wu, Ni Song, Jiaxin Hu, Changxiao Tian, Shucheng Zhang, Hua Wu
A novel PRRSV strain was isolated in China that was genetically similar to the NADC30 strain which is reported to have spread throughout China. The objective of the present study was to evaluate the cross-protective efficacy of the live vaccine TJM-F92 in young pigs against challenge with a NADC30-like strain, HN201605. Twenty-five PRRSV- and antibody-free pigs were randomly divided into the following five groups: Vac/ChA, Unvac/ChA, Vac/ChB, Unvac/ChB and the mock. The pigs in groups Vac/ChA and Vac/ChB were inoculated intramuscularly with 1 mL TJM-F92 (105...
April 2018: Virologica Sinica
Daniel Gonçalves, João Pereira-Vaz, Vitor Duque, Victor Bandeira, Carlos Fonseca, Ana Donato, Cristina Luxo, Ana Miguel Matos
No abstract text is available yet for this article.
April 2018: Virologica Sinica
Zhichao Xu, Huiling Zhong, Qingfeng Zhou, Yunping Du, Li Chen, Yun Zhang, Chunyi Xue, Yongchang Cao
Porcine deltacoronavirus (PDCoV) is a newly identified virus that causes watery diarrhea in newborn piglets and results in significant economic losses to the pig industry. Since first reported in Hong Kong in 2012, PDCoV has been subsequently detected in USA, South Korea, Thailand, and mainland China. Here we isolated a strain of PDCoV, named CHN-GD-2016, from the intestinal content of a diseased newborn piglet with severe diarrhea in a pig farm in Guangdong, China. PDCoV CHN-GD-2016 could be identified by immunofluorescence with PDCoV specific rabbit antisera, and typical crown-shaped particles with spiky surface projections of this PDCoV were observed with electron microscopy...
April 2018: Virologica Sinica
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