Lance P Encell, Rachel Friedman Ohana, Kris Zimmerman, Paul Otto, Gediminas Vidugiris, Monika G Wood, Georgyi V Los, Mark G McDougall, Chad Zimprich, Natasha Karassina, Randall D Learish, Robin Hurst, James Hartnett, Sarah Wheeler, Pete Stecha, Jami English, Kate Zhao, Jacqui Mendez, Hélène A Benink, Nancy Murphy, Danette L Daniels, Michael R Slater, Marjeta Urh, Aldis Darzins, Dieter H Klaubert, Robert F Bulleit, Keith V Wood
Our fundamental understanding of proteins and their biological significance has been enhanced by genetic fusion tags, as they provide a convenient method for introducing unique properties to proteins so that they can be examinedin isolation. Commonly used tags satisfy many of the requirements for applications relating to the detection and isolation of proteins from complex samples. However, their utility at low concentration becomes compromised if the binding affinity for a detection or capture reagent is not adequate to produce a stable interaction...
2012: Current Chemical Genomics