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Beneficial Microbes

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https://www.readbyqxmd.com/read/27903093/challenges-in-simulating-the-human-gut-for-understanding-the-role-of-the-microbiota-in-obesity
#1
M Aguirre, K Venema
There is an elevated incidence of cases of obesity worldwide. Therefore, the development of strategies to tackle this condition is of vital importance. This review focuses on the necessity of optimising in vitro systems to model human colonic fermentation in obese subjects. This may allow to increase the resolution and the physiological relevance of the information obtained from this type of studies when evaluating the potential role that the human gut microbiota plays in obesity. In light of the parameters that are currently used for the in vitro simulation of the human gut (which are mostly based on information derived from healthy subjects) and the possible difference with an obese condition, we propose to revise and improve specific standard operating procedures...
December 1, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27903092/habitual-intake-of-fermented-milk-products-containing-lactobacillus-casei-strain-shirota-and-a-reduced-risk-of-hypertension-in-older-people
#2
Y Aoyagi, S Park, S Matsubara, Y Honda, R Amamoto, A Kushiro, K Miyazaki, R J Shephard
This study investigated relationships between the frequent intake of fermented milk products containing Lactobacillus casei strain Shirota (LcS) and the onset of hypertension (resting systemic pressure ≥140 mmHg [systolic]/≥90 mmHg [diastolic], a doctor's diagnosis and/or antihypertensive medicine use) during a 5-year period in 352 communityliving Japanese aged 65 to 93 years (125 men and 227 women). Initially normotensive subjects were divided into two groups (n=254 and n=98) on the basis of their intake of fermented milk products (<3 or ≥3 times/week, respectively), as estimated during an interview by a certified nutritionist...
December 1, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27903091/quantification-of-fitc-labelled-probiotic-lactobacillus-salivarius-dspv-001p-during-gastrointestinal-transit-in-broilers
#3
J E Blajman, D M Astesana, J A Zimmermann, E Rossler, A Romero Scharpen, A P Berisvil, M V Zbrun, L P Soto, M R Rosmini, L S Frizzo
The knowledge related to the fate of probiotics in the complex environment of the intestinal microbiota in broilers is just beginning to be elucidated; however, it is not yet well understood. A good method to investigate the mechanisms by which probiotics mediate their effects is to mark probiotic bacteria and trace them. The aim of this research was to develop a new method to estimate in vivo fluorescein isothiocyanate (FITC)-labelled Lactobacillus salivarius DSPV 001P counts during passage through the gastrointestinal tract (GIT) of broilers...
December 1, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27903090/pediococcus-acidilactici-lab4-and-lactobacillus-plantarum-lab12-assimilate-cholesterol-and-modulate-abca1-cd36-npc1l1-and-scarb1-in-vitro
#4
F T Lim, S M Lim, K Ramasamy
There is growing interest in the use of probiotic lactic acid bacteria (LAB) for prevention of hypercholesterolaemia. This study assessed the cholesterol lowering ability of Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in growth media. Both LAB yielded >98% (39.2 μg/ml) cholesterol lowering in growth media. Nile Red staining indicated direct assimilation of cholesterol by the LAB. The LAB were then explored for their prophylactic (pre-treatment of HT29 cells with LAB prior to cholesterol exposure) and biotherapeutic (treatment of HT29 cells with LAB after exposure to cholesterol) use against short and prolonged exposure of HT29 cells to cholesterol, respectively...
December 1, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27873547/daily-ingestion-of-the-probiotic-lactobacillus-paracasei-st11-decreases-vaccinia-virus-dissemination-and-lethality-in-a-mouse-model
#5
A C Dos Santos Pereira Andrade, M Teixeira Lima, G Pereira Oliveira, R Silva Calixto, É Lorenna de Sales E Souza, D da Glória de Souza, C M de Almeida Leite, J M Siqueira Ferreira, E G Kroon, D Bretas de Oliveira, F Dos Santos Martins, J S Abrahão
Vaccinia virus (VACV) is an important pathogen. Although studies have shown relationships between probiotics and viruses, the effect of probiotics on VACV infection is unknown. Therefore, this work aims to investigate the probiotics effects on VACV infection. Mice were divided into four groups, two non-infected groups, one receiving the probiotic, the other one not receiving it, and two groups infected intranasally with VACV Western Reserve (VACV-WR) receiving or not receiving the probiotic. Viral titres in organs and cytokine production in the lungs were analysed...
November 22, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27873546/evaluation-of-the-effect-of-soymilk-fermented-by-a-riboflavin-producing-lactobacillus-plantarum-strain-in-a-murine-model-of-colitis
#6
R Levit, G Savoy de Giori, A de Moreno de LeBlanc, J G LeBlanc
Inflammatory bowel diseases (IBD) are idiopathic diseases of the gastrointestinal tract characterised by recurrent inflammation that require lifelong treatments. It has been shown that certain strains of lactic acid bacteria (LAB) can produce specific health-promoting compounds in foods or in the gastrointestinal tract that can in turn prevent and/or treat IBD. This study was designed to evaluate the possible therapeutic potential of soymilk fermented by the riboflavin-producing strain Lactobacillus plantarum CRL 2130 in a trinitrobenzene sulfonic induced colitis mouse model...
November 22, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27873545/presence-of-lactobacillus-reuteri-in-saliva-coincide-with-higher-salivary-iga-in-young-adults-after-intake-of-probiotic-lozenges
#7
G Braathen, V Ingildsen, S Twetman, D Ericson, M R Jørgensen
The aim of this study was to compare the concentration of salivary immunoglobulin A (IgA) and the selected interleukins (IL)-1β, IL-6, IL-8 and IL-10 in young individuals with presence and non-presence of Lactobacillus reuteri in saliva after a three-week intervention with probiotic lozenges. The study group consisted of 47 healthy individuals aged 18-32 years with no clinical signs of oral inflammation. In a randomised, double-blind, placebo-controlled, cross-over trial participants ingested two lozenges per day containing two strains of the probiotic bacterium L...
November 22, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27824278/modulation-of-porcine-intestinal-epitheliocytes-immunetranscriptome-response-by-lactobacillus-jensenii-tl2937
#8
H Kobayashi, L Albarracin, N Sato, P Kanmani, A K M H Kober, W Ikeda-Ohtsubo, Y Suda, T Nochi, H Aso, S Makino, H Kano, S Ohkawara, T Saito, J Villena, H Kitazawa
In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27824277/novel-bioactive-from-lactobacillus-brevis-dsm17250-to-stimulate-the-growth-of-staphylococcus-epidermidis-a-pilot-study
#9
C Holz, J Benning, M Schaudt, A Heilmann, J Schultchen, D Goelling, C Lang
Commensal skin microbiota plays an important role in both influencing the immune response of the skin and acting as a barrier against colonisation of potentially pathogenic microorganisms and overgrowth of opportunistic pathogens. Staphylococcus epidermidis is a key constituent of the normal microbiota on human skin. It balances the inflammatory response after skin injury and produces antimicrobial molecules that selectively inhibit skin pathogens. Here we describe Lactobacillus brevis DSM17250 that was identified among hundreds of Lactobacillus strains to exhibit an anti-inflammatory effect in human keratinocytes in vitro and specific stimulatory impact on the growth of S...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27824276/identification-of-a-new-small-bioactive-peptide-from-lactobacillus-gasseri-supernatant
#10
A Zanfardino, G Criscuolo, B Di Luccia, E Pizzo, M L Ciavatta, E Notomista, A Carpentieri, A Pezzella, M Varcamonti
Ten lactic acid bacteria (LAB) strains, previously isolated from human ileal biopsy of healthy volunteers, were screened for production and secretion of molecules having anti-bacterial and anti-biofilm activities. Because many recent reports indicate that LAB secreted molecules may exert immune-modulatory action, we also tested the effect on human intestinal HCT116 cells challenged with bacterial lipopolysaccharides. One of the Lactobacillus gasseri strains, SF1109, strongly inhibited: (1) Pseudomonas aeruginosa growth; (2) Escherichia coli biofilm production; (3) LPS induction of P-ERK1/2 in HCT116 cells, and was selected for further characterisation of the secreted active molecule...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27824275/potential-influence-of-dairy-propionibacteria-on-the-growth-and-acid-metabolism-of-streptococcus-bovis-and-megasphaera-elsdenii
#11
J Luo, C S Ranadheera, S King, C A Evans, S K Baines
Ruminal acidosis is a prevalent disorder among dairy cows and feedlot cattle, which can significantly impair their health and productivity. This study, involving seven different strains of dairy propionibacteria, represents an in vitro investigation of the feasibility of using these organisms as direct-fed microbials to control lactic acid acumulation in the rumen. Interactions between the propionibacteria, Streptococcus bovis and Megasphaera elsdenii were evaluated in terms of effects on lactic, acetic and propionic acid metabolism, following co-incubation...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27824274/in-vitro-colonisation-of-the-distal-colon-by-akkermansia-muciniphila-is-largely-mucin-and-ph-dependent
#12
F Van Herreweghen, P Van den Abbeele, T De Mulder, R De Weirdt, A Geirnaert, E Hernandez-Sanabria, R Vilchez-Vargas, R Jauregui, D H Pieper, C Belzer, W M De Vos, T Van de Wiele
Host mucin is the main constituent of the mucus layer that covers the gut epithelium of the host, and an important source of glycans for the bacteria colonising the intestine. Akkermansia muciniphila is a mucin-degrading bacterium, abundant in the human gut, that is able to produce acetate and propionate during this degradation process. A. muciniphila has been correlated with human health in previous studies, but a mechanistic explanation is lacking. In this study, the main site of colonisation was characterised alongside additional conditions, such as differences in colon pH, prebiotic supplementation and variable mucin supply...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27824273/anti-inflammaging-effects-of-lactobacillus-brevis-ow38-in-aged-mice
#13
J-J Jeong, K A Kim, Y-J Hwang, M J Han, D-H Kim
In the present study, lactic acid bacteria (LAB) strains were collected from kimchi and were screened to isolate strains that inhibit lipopolysaccharide (LPS) production by Escherichia coli and p16 expression and nuclear factor-kappa B (NF-κB) activation in LPS-stimulated macrophages. Oral administration of Lactobacillus brevis OW38 (1×10(9) cfu/mouse) to aged mice (male, 18 months old) for 8 weeks reduced the LPS level in colon fluid and blood. In addition, OW38 treatment also reduced the ratio of Firmicutes or Proteobacteria to Bacteroidetes, which was significantly higher in aged mice than in young mice...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27824272/in-vivo-effects-of-s-pantoprazole-polaprenzinc-and-probiotic-blend-on-chronic-small-intestinal-injury-induced-by-indomethacin
#14
S J Byun, T J Lim, Y J Lim, J G Seo, M J Chung
Treatment and prevention methods for non-steroidal anti-inflammatory drug-induced enteropathy have not yet been established. We tested the preventive effects of s-pantoprazole sodium trihydrate (PAN), polaprezinc (PZ), and probiotics on an indomethacin (Indo)-induced small intestinal injury in a rat model. Rats were randomised into 6 groups to receive: normal saline (control), Indo (6 mg/kg), PZ plus Indo, PAN plus Indo, or probiotics plus Indo (at 10(8) and 10(9) cfu/head) for 2 weeks. We measured body weight, food intake, severity of small intestinal damage, haemoglobin (Hb) levels in the small intestinal fluid, intestinal inflammatory cytokines, and a few groups of faecal bacteria...
November 8, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27726419/effects-of-in-ovo-supplementation-of-probiotics-on-performance-and-immunocompetence-of-broiler-chicks-to-an-eimeria-challenge
#15
C M Pender, S Kim, T D Potter, M M Ritzi, M Young, R A Dalloul
Coccidiosis is regarded as the parasitic disease with the greatest economic impact on the poultry industry due to reduced performance and increased mortality. A study was conducted to investigate the effects of in ovo administration of probiotics on hatchability, performance, immune organ weights, and lesion scores in broiler chicks during a mixed Eimeria infection. At embryonic day 18, 210 eggs were injected with either sterile water or 1×10(6) cfu probiotic bacteria. On day 3 post-hatch, half of the chicks from each treatment group were challenged with a mixed inoculum of Eimeria acervulina, Eimeria maxima and Eimeria tenella...
October 11, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27680209/feed-supplementation-with-avian-propionibacterium-acidipropionici-contributes-to-mucosa-development-in-early-stages-of-rearing-broiler-chickens
#16
E Argañaraz Martínez, J D Babot, M J Lorenzo-Pisarello, M C Apella, A Perez Chaia
Different studies in animal rearing claim the probiotic potential of species of the genus Propionibacterium. The effects of strains of Propionibacterium acidipropionici isolated from poultry intestine on microbiota activity and intestinal mucosa development were investigated in the early stage of rearing chicks and the safety of the dose used was investigated. The strains P. acidipropionici LET105 and LET107, administered as monoculture to chicks from the 1(st) to 14(th) day of life in a daily dose of 106 cfu/ml administered in the drinking water resulted harmless...
September 29, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27680208/the-different-effects-of-probiotics-treatment-on-salmonella-induced-interleukin-8-response-in-intestinal-epithelia-cells-via-pi3k-akt-and-nod2-expression
#17
F-C Huang, S-C Huang
Salmonella spp. remains a major public health problem for the whole world. Intestinal epithelial cells serve as an essential component of the innate mucosal immune system to defend against Salmonella infection. A substantial amount of evidence has accumulated that probiotics can regulate interleukin 8 (IL-8) involved in innate immunity. However, the exact effect of probiotics on epithelial IL-8 response to Salmonella infection is not well understood. Therefore, we investigated the action of probiotics on Salmonella-infected Caco-2 cells and its novel mechanisms...
September 29, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27633175/the-effect-of-a-probiotic-escherichia-coli-strain-on-regulatory-t-cells-in-six-year-old-children
#18
J Hrdý, I Kocourková, R Lodinová-Žádníková, L Kolářová, L Prokešová
Probiotics are believed to prevent or reduce allergy development but the mechanism of their beneficial effect is still poorly understood. Immune characteristics of regulatory T cells (Tregs) in peripheral blood of perinatally probiotic-supplemented children of allergic mothers (51 children), non-supplemented children of allergic mothers (42 children), and non-supplemented children of healthy mothers (28 children) were compared at the age of 6-7 years. A first dose of a probiotic Escherichia coli strain (E. coli O83:K24:H31) was administered within 2 days after the birth and then 12 times during the first months of life and children were followed longitudinally...
September 16, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27633174/the-effect-of-probiotic-treatment-on-elderly-patients-with-distal-radius-fracture-a-prospective-double-blind-placebo-controlled-randomised-clinical-trial
#19
M Lei, L-M Hua, D-W Wang
Probiotic treatment has been shown to improve bone formation, increase bone mass density and prevent bone loss. We aimed to assess the effect of probiotic treatment on functional recovery in elderly patients with a distal radius fracture. A total of 417 elderly patients with an acute distal radius fracture were enrolled in this double-blind placebo-controlled clinical trial. They were randomised to receive skimmed milk containing either a commercial probiotic (Lactobacillus casei Shirota) or placebo daily for a period of 6 months after the fracture...
September 16, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27633173/citrus-juice-fermented-with-lactobacillus-plantarum-yit-0132-alleviates-symptoms-of-perennial-allergic-rhinitis-in-a-double-blind-placebo-controlled-trial
#20
N Harima-Mizusawa, M Kano, Nozaki, C Nonaka, K Miyazaki, T Enomoto
This study aimed to examine whether citrus juice fermented with Lactobacillus plantarum YIT 0132 (LP0132), which was pasteurised after fermentation, could alleviate the symptoms of perennial allergic rhinitis in a double-blind, placebo-controlled, parallel-group trial. Subjects with perennial allergic rhinitis consumed LP0132-fermented juice (n=17) or unfermented citrus juice (placebo; n=16) once a day for 8 weeks. During the pre-intervention and intervention periods, the subjects recorded nasal symptoms (number of sneezing attacks, number of nose-blowing incidents, and stuffy nose score)...
September 16, 2016: Beneficial Microbes
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