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Biopreservation and Biobanking

Qiyuan Li, Xian Wang, Xiaopan Liu, Qiuyan Liao, Jianbo Sun, Xuheng He, Ting Yang, Jiefang Yin, Jia Jia, Xue Li, Marthe Colotte, Jacques Bonnet
RNA is an essential biological material for research in genomics and translational medicine. As such, its storage for biobanking is an important field of study. Traditionally, long-term storage in the cold (generally freezers or liquid nitrogen) is used to maintain high-quality (in terms of quantity and integrity) RNA. Room temperature (RT) preservation provides an alternative to the cold, which is plagued by serious problems (mainly cost and safety), for RNA long-term storage. In this study, we evaluated the performance of several RT storage procedures, including the RNAshell(®) from Imagene, where the RNA is dried and kept protected from the atmosphere, and the vacuum drying of RNA with additives such as the Imagene stabilization solution and a home-made trehalose solution...
October 12, 2017: Biopreservation and Biobanking
Guobo Quan, Guoquan Wu, Qionghua Hong
The establishment of a cryopreservation procedure for sheep oocytes will enhance the development of long-term conservation of sheep female genetic resources and the advancement of sheep embryonic biotechnology. However, higher cytoplasmic lipid content and complex subcellular structures affect the relatively higher sensitivity of oocytes to chilling and freezing stresses. Currently, the reports related to sheep oocyte cryopreservation lag behind bovine or pig studies. A standardized freezing procedure has not been established...
October 5, 2017: Biopreservation and Biobanking
Dayong Gao, Zisis Kozlakidis, Brent Schacter, Jim Vaught
No abstract text is available yet for this article.
September 28, 2017: Biopreservation and Biobanking
Catherine Y Seiler
No abstract text is available yet for this article.
September 22, 2017: Biopreservation and Biobanking
Xiaoli Ji, Min Wang, Lingling Li, Fang Chen, Yanyang Zhang, Qian Li, Junmei Zhou
High-quality biosamples are valuable resources for biomedical research. However, some tissues are stored without being sectioned into small aliquots and have to undergo repeated freeze-thaw cycles throughout prolonged experimentation. Little is known regarding the effects of repeated freeze-thaw cycles on the quality of biomolecules in tissues. The aim of this study was to evaluate the impact of repeated freeze-thaw (at room temperature or on ice) cycles on biomolecules and gene expression in four different types of tissues...
September 20, 2017: Biopreservation and Biobanking
Emmanuelle Gormally, Isabelle Hardy, Elodie Caboux, Jeanne-Hélène di Donato, Pierre Hainaut, Paul Hofman
The growing complexity of biobanking requires dedicated professional staff who are trained in multiple aspects of the biobanking process, including technical, managerial, regulatory, and ethical aspects, and who have a good understanding of the challenges of biospecimen research, but also of the challenges related to the sustainability of future biobanks. Up to the present, biobanking staff have been trained in an ad-hoc manner, usually through specific short duration courses, for example, summer schools. In this article, we describe the development/establishment of a systematic 2-year training program at the Master level intended for students with a background in life sciences and providing them with a professional qualification as a "Biobank Manager...
September 18, 2017: Biopreservation and Biobanking
Lívia C Magalhães, Maajid H Bhat, Jeferson L S Freitas, Luciana M Melo, Dárcio I A Teixeira, Luiz C A Pinto, Lília M C Câmara, José M B Duarte, Vicente J F Freitas
The brown brocket deer Mazama gouazoubira is 1 of the 10 recognized brocket deer of the Neotropical region. Recently, this species has suffered a population decline due to current threats, mainly poaching and habitat loss. Several studies have shown that some endangered species can benefit from interspecies somatic cell nuclear transfer technology through the use of their somatic cells, such as the fibroblasts. Thus, the aim of this study was to verify the viability and the effect of cryopreservation on fibroblasts after several passages...
September 18, 2017: Biopreservation and Biobanking
Jae-Eun Lee, Donghyok Kwon, Meehee Lee, Young-Youl Kim
No abstract text is available yet for this article.
September 15, 2017: Biopreservation and Biobanking
Takehiro Matsubara, Shuta Tomida, Junichi Soh, Takahiro Uwabo, Yoshiko Mori, Mizuki Morita, Yasutomo Nasu, Susumu Kanazawa, Shinichi Toyooka
No abstract text is available yet for this article.
September 14, 2017: Biopreservation and Biobanking
Murat Sariyar, Stephanie Suhr, Irene Schlünder
The rising demand to use genetic data for research goes hand in hand with an increased awareness of privacy issues related to its use. Using human genetic data in a legally compliant way requires an examination of the legal basis as well as an assessment of potential disclosure risks. Focusing on the relevant legal framework in the European Union, we discuss open questions and uncertainties around the handling of genetic data in research, which can result in the introduction of unnecessary hurdles for data sharing...
September 7, 2017: Biopreservation and Biobanking
Takahiro Yamanaka, Teppei Goto, Masumi Hirabayashi, Shinichi Hochi
The practical requirements of islet transplantation necessitate that a large quantity of pancreatic islets be cryopreserved for a long period of time in a simple and convenient manner. We cryopreserved rat islets (size range 101-150 μm in mean diameter) by vitrification with either a Cryotop(®) device or a ø = 57-μm nylon mesh device in units of 10 islets, or by conventional freezing with a Bicell(®) vessel in units of 50 islets. Postwarm/thaw survival rates of the islets were 68.1% ± 5.9%, 64...
September 5, 2017: Biopreservation and Biobanking
Mahsa Shabani, Edward S Dove, Madeleine Murtagh, Bartha Maria Knoppers, Pascal Borry
Discussions regarding responsible genomic data sharing often center around ethical and legal issues such as the consent, privacy, and confidentiality of individuals, families, and communities. To ensure the ethical grounds of genomic data sharing, oversight by both research ethics and Data Access Committees (DACs) across the research lifecycle is warranted. In this article, we review these oversight practices and argue that they reveal a compelling need to clarify the scope of ethical considerations by oversight bodies and to delineate core elements such as "objectionable" data uses...
August 24, 2017: Biopreservation and Biobanking
Yu Huang, Jianye Wang, Xiaoming Zhou, Ji Peng, Zhengliang Zhang, Lingxiao Shen, Frankliu Gao, Yunxia Cao
The currently used commercial cooling-rate control device is the liquid nitrogen controlled rate freezer (LNF), which has some shortcomings such as high cost, high liquid nitrogen consumption, and potential operational risks in quality control. Based on thermophysical properties of new materials, we improved, manufactured, and optimized a reliable yet simple device named the "passive cooling rate-controlled device (PCD)" with real-time temperature tracing. In this study, using the improved PCD we cryopreserved human umbilical vein endothelial cells (HUVECs) and compared the results with a standard commercial CryoMed LNF...
August 22, 2017: Biopreservation and Biobanking
Fatemeh Mohammadipanah, Leila Parvizi, Javad Hamedi, Farzaneh Azizmohseni
Streptomyces sp. are bacteria recognized as the producers of more than half of the known bioactive compounds. Developing appropriate preservation methods for industrial strains of Streptomyces is necessary, as continuous subculture could have significant negative effects on their characteristics, including their potential to produce secondary metabolites. The effects of two common preservation methods on a bioactive metabolite producer, Streptomyces zagrosensis UTMC 1154, were studied. In the cryopreservation method, glycerol and dimethyl sulfoxide (DMSO) were evaluated as cryoprotectants...
August 18, 2017: Biopreservation and Biobanking
Harriëtte Oldenhof, Judith Bigalk, Christiane Hettel, Lawrence de Oliveira Barros, Bulat Sydykov, Árpád Cs Bajcsy, Harald Sieme, Willem F Wolkers
In this study, modeling and experimental approaches were used to investigate the interplay between cooling rate and protectant concentration for cryopreservation of stallion sperm. Glycerol (GLY), ethylene glycol (EG), dimethylformamide (DMF), propylene glycol (PG), and dimethyl sulfoxide (DMSO) were tested as cryoprotective agents (CPAs), using concentrations up to 1500 mM and cooling rates ranging from 5°C to 55°C min(-1). Modeling of the extent of sperm dehydration during freezing was done using previously determined values of the sperm membrane permeability to water to predict optimal cooling rates for cryopreservation...
August 14, 2017: Biopreservation and Biobanking
Jiaji Pan, Zhiquan Shu, Shen Ren, Dayong Gao
In the rewarming process during cryopreservation, preventing ice recrystallization and thermal stress is important, especially for large tissues and organs. Uniform and rapid heating is essential in ameliorating the problem and maintaining the viability of cryopreserved biological samples. Currently, the most promising method is heating by application of electromagnetic (EM) waves, the effectiveness of which is dependent on the dielectric properties (DP) of the cryopreserved materials. In this work, the cavity perturbation method was adopted to measure the DP of cryoprotectant solutions...
August 7, 2017: Biopreservation and Biobanking
Weiwei Qin, Chan Zhao, Linpei Zhang, Ting Wang, Youhe Gao
Tears covering the ocular surface are important biofluids containing thousands of molecules, including proteins, lipids, metabolites, nucleic acids, and electrolytes. Tears are valuable resources for biomarker research of ocular and even systemic diseases. For application in biomarker studies, tear samples should ideally be stored using a simple, lowcost, and efficient method along with the patient's medical records. For this purpose, we developed a novel Schirmer's strip-based dry method that allows for storage of tear samples in vacuum bags at room temperature...
July 26, 2017: Biopreservation and Biobanking
Marianna J Bledsoe
No abstract text is available yet for this article.
August 2017: Biopreservation and Biobanking
Clare M Allocca, Marianna J Bledsoe, Koh Furuta, Nilsa C Ramirez
No abstract text is available yet for this article.
August 2017: Biopreservation and Biobanking
Na He, Yan Guo, Min He, Wanmin Qiang, Haixin Li
BACKGROUND: High-quality biospecimen collection from consented patients is crucial for cancer research activities. Patients' attitudes and willingness toward specimen donation influence high-quality biospecimen collection for cancer research activities. METHODS: We carried out a cross-sectional study among randomly selected patients from 11 cancer departments of Tianjin Medical University Cancer Institute and Hospital between August 2014 and August 2015. A total of 784 patients were included to complete a 30-item self-administered survey...
August 2017: Biopreservation and Biobanking
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