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Biopreservation and Biobanking

Rebecca O Barnes, Katheryn E Shea, Peter H Watson
As health research increasingly relies on biospecimens and associated data, new demands have emerged for biorepositories to provide assurances of the quality of their overall operations, not just assurances of the quality of the biospecimens and data that they hold. The biobanking community has responded in various ways, including the creation of two different programs to disseminate biobanking best practices. This article describes in detail the Canadian Tissue Repository Network (CTRNet) Biobank Certification Program and the College of American Pathologists (CAP) Biorepository Accreditation Program...
October 14, 2016: Biopreservation and Biobanking
Rafael Calixto Bortolin, Juciano Gasparotto, Amanda Rodrigues de Vargas, Maurilio da Silva Morrone, Alice Kunzler, Bernardo Saldanha Henkin, Paloma Rodrigues Chaves, Sabrina Roncato, Daniel Pens Gelain, José Cláudio Fonseca Moreira
Most scientific studies are too long to be conducted in a single day or even in a few days. Thus, there is a need to store samples for subsequent investigations. There is sparse information about specific sample storage protocols that minimize analytical error and variability in evaluations of redox parameters. Therefore, the effects of storage temperature and freezing time on enzymatic activities, protein oxidative damage, and CAT (catalase) and SOD1 (superoxide dismutase) immunocontent of blood, liver, and brain from rats were determined for two different sample forms (frozen homogenized tissue or frozen intact tissue)...
September 23, 2016: Biopreservation and Biobanking
Wei Liang, Xuexun Zhou, Lan Yao, Baolin Liu
The preservation of DNA, RNA, and protein markers in biological specimens is essential for initial diagnosis, subsequent verification, and comparison, as well as for archival retention of pathological materials in modern molecular diagnostics and precision medicine. Considerable attention has been paid to the methods of collection, handling, and preparation of specimens for initial testing, but insufficient attention to the long-term specimen preservation for later verification, comparison, and archival retention...
September 12, 2016: Biopreservation and Biobanking
Lothar Lauterboeck, Debapriya Saha, Anamika Chatterjee, Nicola Hofmann, Birgit Glasmacher
In the previous decade, numerous biobanks were established and have created large markets for the storage of bioactive compounds, cells, and tissues for medical and diagnostic applications. For in vivo clinical and therapeutic purposes, it is critical to use well-defined and xeno-free components during cultivation, preservation, and transplantation of biological material. Safe and efficacious storage of bioactive molecules, cells, and tissues, without the addition of undefined medium components, minimizes risks of zoonotic disease transmission and is thus an essential and desirable prerequisite for biobanks...
September 7, 2016: Biopreservation and Biobanking
Lise Matzke, Sindy Babinszky, Alex Slotty, Anna Meredith, Tania Castillo-Pelayo, Marianne Henderson, Daniel Simeon-Dubach, Brent Schacter, Peter H Watson
The notion of attributing user fees to researchers for biospecimens provided by biobanks has been discussed frequently in the literature. However, the considerations around how to attribute the cost for these biospecimens and data have, until recently, not been well described. Common across most biobank disciplines are similar factors that influence user fees such as capital and operating costs, internal and external demand, and market competition. A biospecimen user fee calculator tool developed by CTRNet, a tumor biobank network, was published in 2014 and is accessible online at www...
August 30, 2016: Biopreservation and Biobanking
William E Grizzle, Dennis Otali, Katherine C Sexton, Daniel S Atherton
Frequently investigators request that tissues be collected and processed in less than one hour following removal from a patient. Some biorepositories expend significant personnel time and other resources in trying to meet such goals; however, it is unclear whether the perceived benefits of relatively short cold ischemia times warrant these added costs. The literature of human surgical tissues prospectively exposed to cold ischemia at several time points was reviewed to compare the changes in transcripts/genes and microRNA with time of cold ischemia...
August 23, 2016: Biopreservation and Biobanking
Conny Mathay, Gaël Hamot, Estelle Henry, Kathleen Mommaerts, Audur Thorlaksdottir, Johanna Trouet, Fay Betsou
BACKGROUND: This article is the fifth in a series of publications providing formal method validation for biospecimen processing. We report the optimization and validation of methodology to obtain nucleic acids of sufficient quantity and quality from blood. METHODS: DNA was extracted using the Chemagic DNA Blood Kit on an MSM I. Extraction was optimized in terms of blood volume, elution buffer volume, and lysis conditions. The optimal protocol was validated for reproducibility, robustness (delay to buffy coat extraction, blood vs...
August 22, 2016: Biopreservation and Biobanking
Laura Calleros-Basilio, María Alicia Cortés, Andrea García-Jerez, Alicia Luengo-Rodríguez, Ana Orozco-Agudo, José Manuel Valdivielso, Diego Rodríguez-Puyol, Manuel Rodríguez-Puyol
BACKGROUND: Biobanks are useful platforms to build bridges between basic, translational, and clinical research and clinical care. They are repositories of high-quality human biological samples ideal for evaluating their histological characteristics and also their genome, transcriptome, and proteome. The Spanish Renal Research Network Biobank contains more than 76,500 well-preserved frozen samples of a wide variety of kidney diseases, collected from 5450 patients seen by over 70 nephrology services throughout the Spanish territory...
August 19, 2016: Biopreservation and Biobanking
Xiaoli Liu, Gang Zhao, Zhiquan Shu, Dan Niu, Zhiguo Zhang, Ping Zhou, Yunxia Cao, Dayong Gao
Quantitative evaluation of the inherent correlation between cell cryoinjuries and intracellular ice formation (IIF) together with recrystallization (IIR) is of primary importance for both optimization of biopreservation and cryotherapy. The objective of this study is to thoroughly explore the roles of IIF on cell viability by using pig iliac endothelium cells (PIECs) as model cells during freezing and thawing. The experimental results indicated that both the probabilities of IIF (PIF) and IIR (PIR) increased along with the increase of cooling rates (p < 0...
August 17, 2016: Biopreservation and Biobanking
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October 2016: Biopreservation and Biobanking
Marianna J Bledsoe, Marianne Henderson, Anne-Marie Tassé, Bartha M Knoppers
No abstract text is available yet for this article.
October 2016: Biopreservation and Biobanking
Jim Vaught
No abstract text is available yet for this article.
October 2016: Biopreservation and Biobanking
Jim Vaught, Sheryl Nale
No abstract text is available yet for this article.
August 2016: Biopreservation and Biobanking
Jim Vaught
No abstract text is available yet for this article.
August 2016: Biopreservation and Biobanking
Federica Napolitani, Alessia Calzolari, Anne Cambon-Thomsen, Laurence Mabile, Anna Maria Rossi, Paola De Castro, Elena Bravo
Even though an increasing portion of biomedical research today relies on the use of bioresources, at present biobankers are not able to trace this use in scientific literature and measure its impact with a variety of citation metrics. The "BRIF (Bioresource Research Impact Factor) and journal editors" subgroup was created precisely with the aim to study this issue and to build a standardized system to cite bioresources in journal articles. This report aims at presenting a guideline for Citation of BioResources in journal Articles (CoBRA)...
August 2016: Biopreservation and Biobanking
Gema Lucena-Aguilar, Ana María Sánchez-López, Cristina Barberán-Aceituno, José Antonio Carrillo-Ávila, José Antonio López-Guerrero, Rocío Aguilar-Quesada
High-quality human DNA samples and associated information of individuals are necessary for biomedical research. Biobanks act as a support infrastructure for the scientific community by providing a large number of high-quality biological samples for specific downstream applications. For this purpose, biobank methods for sample preparation must ensure the usefulness and long-term functionality of the products obtained. Quality indicators are the tool to measure these parameters, the purity and integrity determination being those specifically used for DNA...
August 2016: Biopreservation and Biobanking
Sarah Nejlund, Julie Smith, Jaco Kraan, Henrik Stender, Mai N Van, Sven T Langkjer, Mikkel T Nielsen, György Sölétormos, Thore Hillig
BACKGROUND: A blood sample containing circulating tumor cells (CTCs) may serve as a surrogate for metastasis in invasive cancer. Cryopreservation will provide new opportunities in management of clinical samples in the laboratory and allow collection of samples over time for future analysis of existing and upcoming cancer biomarkers. METHODS: Blood samples from healthy volunteers were spiked with high (∼500) and low (∼50) number of tumor cells from culture. The samples were stored at -80C with cryopreservative dimethyl sulfoxide mixed with Roswell Park Memorial Institute 1640 medium...
August 2016: Biopreservation and Biobanking
Kelly A Edwards, Sharon F Terry, Dana Gold, Elizabeth J Horn, Mary Schwartz, Molly Stuart, Suzanne D Vernon
Biobanks are increasingly powerful tools used in translational research, and disease advocacy organizations (DAOs) are making their presence known as research drivers and partners. We examined DAO approaches to biobanking to inform how the enterprise of biobanking can grow and become even more impactful in human health. In this commentary, we outline overarching approaches from successful DAO biobanks. These lessons learned suggest principles that can create a more participant-centric approach and illustrate the key roles DAOs can play as partners in research initiatives...
August 2016: Biopreservation and Biobanking
Alexander Wurm, Ruth Steiger, Christoph G Ammann, David Putzer, Michael C Liebensteiner, Michael Nogler, Débora C Coraça-Huber
METHODOLOGY: We determined the content of amide I, amide III, PO4, CO3, and CH2 in samples of fresh bone, bone frozen at -80°C thawed once, bone after two freeze-thaw cycles, and chemically cleaned bone chips. A total of 750 Raman spectra were collected per sample group and the derived quantitative values compared statistically by one-way ANOVA. RESULTS: We found statistically significant differences between the investigated sample groups differing in their treatment already after one freeze-thaw cycle and as well after multiple freeze-thaw cycles, and/or chemical cleaning...
August 2016: Biopreservation and Biobanking
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