Read by QxMD icon Read

Biopreservation and Biobanking

Michaela Th Mayrhofer, Irene Schlünder
With the ethical, legal, and societal issues (ELSI) Knowledge Base, we introduce a key element of the Biobanking and Biomolecular Resources Research Infrastructure-European Research Infrastructure Consortium (BBMRI-ERIC) Common Service ELSI, which provides ethical, legal, and societal support for researchers and biobankers involved in transnational research. In contrast to the customized support provided by the ELSI Helpdesk, the ELSI Knowledge Base will be available to the user on a self-serve basis. The information that is made available through a knowledge base comes from multiple sources, usually from several expert contributors who are well versed in the subject matter...
August 13, 2018: Biopreservation and Biobanking
José Carlos Ferreira-Silva, Sarah Romini Lima Basto, Marcelo Tigre Moura, Jorge Motta Rocha, Leopoldo Mayer Freitas Neto, José Pompeu Santos Filho, Manoel Lopes Silva Filho, Marcos Antonio Lemos Oliveira
The work described here aimed to verify the efficiency of different extenders for cryopreservation of equine semen using sperm motility and acrosin activity as spermatic parameters. The semen was fractioned into two equal parts and resuspended in an 11% lactose solution in a 1:1 proportion, where it remained for 20 minutes at room temperature. The semen was centrifuged at 600 g for 10 minutes, and after the second centrifugation, each pellet received the freezing extender (Merck or Zorlesco) and was loaded into 4 mL straws...
July 30, 2018: Biopreservation and Biobanking
Xiaowei Shi, Huanhuan Hu, Guojie Ji, Jing Zhang, Rui Liu, Han Zhang, Mingwen Li
Currently the most popular mouse sperm freezing medium is R18SM3+MTG containing 18% raffinose, 3% skim milk, and 0.5 mM monothioglycerol (MTG), but there is no information available about whether MTG and other antioxidants can cryoprotect mouse sperm DNA integrity. It is also uncertain if sucrose can be used successfully for sperm cryopreservation. In this report we compared the cryoprotective effects of sucrose and raffinose, as well as the antioxidants MTG, reduced glutathione (GSH), and quercetin on sperm motility, DNA integrity, and fertility in the C57BL/6J mouse strain...
July 23, 2018: Biopreservation and Biobanking
Sarah R Greytak, Kelly B Engel, Helen M Moore
No abstract text is available yet for this article.
July 13, 2018: Biopreservation and Biobanking
David Lewandowski
No abstract text is available yet for this article.
July 13, 2018: Biopreservation and Biobanking
Gesine Pless-Petig, Ursula Rauen
AIMS: The use of primary hepatocytes in pharmacological and toxicological research as well as for clinical and biotechnological applications requires adequate storage options for these cells. However, hepatocytes are very susceptible to cryopreservation injury. Based on experience in hypothermic storage of hepatocytes, we, in this study, aimed to optimize hepatocyte cryopreservation. MATERIALS AND METHODS: Rat hepatocytes were cryopreserved in serum-containing cell culture medium or in serum-free solutions optimized for hypothermic storage, all with 10% dimethyl sulfoxide, using a standard protocol (-1°C/min in a controlled-rate freezer)...
July 3, 2018: Biopreservation and Biobanking
Beyza Goncu, Emrah Yucesan, Burcu Ozdemir, Harun Basoglu, Nur Ozten Kandas, Fahri Akbas, Erhan Aysan
BACKGROUND: Cold ischemia protects organs and tissues by slowing their metabolism, but it also causes ischemic injury. Minimizing cold ischemia has been an important goal in parathyroid auto- and allotransplantation, as well as the transplantation of other major organs. Parathyroid glands are responsible for calcium homeostasis by releasing parathormone (PTH) into the blood circulation. Functionality of a new parathyroid transport solution (NPTS) and effects on cell viability, PTH secretion, and calcium-sensing receptor (CaSR) levels during cold ischemia were evaluated...
July 2, 2018: Biopreservation and Biobanking
Shaunak Phatak, Harishankar Natesan, Jeunghwan Choi, Kelvin G M Brockbank, John C Bischof
Cryopreservation represents one if not the only long-term option for tissue and perhaps future organ banking. In one particular approach, cryopreservation is achieved by completely avoiding ice formation (or crystallization) through a process called vitrification. This "ice-free" approach to tissue banking requires a combination of high-concentration cryoprotective additives such as M22 (9.4 M), VS55 (8.4 M), or DP6 (6 M) and sufficiently fast rates of cooling and warming to avoid crystallization...
June 29, 2018: Biopreservation and Biobanking
Luciana M Silva, Gildas T Mbemya, Denise D Guerreiro, Danielle Cristina C Brito, Nathalie J Donfack, Maria Luana G S Morais, Giovanna Q Rodrigues, Jamily B Bruno, Rebeca M P Rocha, Benner G Alves, Gary A Apgar, Francielli W S Cibin, José Ricardo Figueiredo, Ana Paula R Rodrigues
AIM: The present study evaluates the effect of different concentrations of antioxidants (catalase - CAT and alpha lipoic acid - ALA) on the follicular activation and morphology, DNA damage, ROS production, and mitochondrial activity in vitrified sheep ovarian tissue. METHODS: This experiment was divided into two steps. First, ovarian fragments were distributed into the following treatments: fresh tissue or control (CTR), incubation (INC), vitrification without antioxidant (VWA), with CAT (10, 20, or 40 IU mL-1 ) or ALA (25, 50, or 100 μM mL-1 )...
June 29, 2018: Biopreservation and Biobanking
Sarah R Greytak, Kelly B Engel, Erik Zmuda, Esmeralda Casas-Silva, Ping Guan, Katherine A Hoadley, Andrew J Mungall, David A Wheeler, Harsha V Doddapaneni, Helen M Moore
No abstract text is available yet for this article.
June 19, 2018: Biopreservation and Biobanking
Jia Luo, Hongkang Zhou, Libing Rao, Tianxiong Liu, Lian Liu, Yanliang Chen, Jianlong Wang, Tu Hu, Shiqi Liu, Dan Chen, Hui Wang, Leping Zeng, JuFang Huang
OBJECTIVES: We have explored a better method to preserve and store human medically amputated large size samples. The approach involved developing a special embalming solution and procedures for biopreservation and biostorage of a large-sized sample as a whole specimen rather than dissected small parts. Evaluation of the effect of our special embalming solution and procedures on whole human amputated extremities compared with excised small tissues was conducted. Histological and morphological techniques and elemental analyses were utilized to assess the effects of our new method using the special embalming solution...
June 11, 2018: Biopreservation and Biobanking
Jan-Eric Litton
Biobanking and BioMolecular resources Research Infrastructure (BBMRI)- European Research Infrastructure Consortium (ERIC) is the largest infrastructure launched in Europe in health research. By nature it is a distributed infrastructure, in which biological samples and data are hosted by the European Member States biobanks. As of today, BBMRI-ERIC consists of 19 European Member States and 1 international organization, the International Agency for Research on Cancer. This means that BBMRI-ERIC has a population of >500 million individuals in Europe...
May 21, 2018: Biopreservation and Biobanking
Nana Cecilie Halmsted Kongsholm, Søren Tvorup Christensen, Janne Rothmar Hermann, Lars Allan Larsen, Timo Minssen, Lotte Bang Pedersen, Neethu Rajam, Niels Tommerup, Aaro Tupasela, Jens Schovsbo
Most university biobanks begin like other university research projects, that is, with an idea conceived by an individual researcher in pursuit of his/her own research interests, publications, funding, and career. Some biobanks, however, come to have scientific value that goes beyond the projects that were initially responsible for the collection of the samples and data they contain. Such value may derive from among other things the uniqueness of the samples in terms of their sheer volume, the quality of the samples, the ability to link the samples with information retrieved in disease registries, or the fact that the samples represent very rare diseases...
August 2018: Biopreservation and Biobanking
Sook Young Yoon, Jin Hee Eum, Soo Kyoung Cha, Tae Ki Yoon, Dong Ryul Lee, Woo Sik Lee
This study investigates the possible causes for low development of blastocysts in vitrified immature oocytes by evaluating the changes of mitochondrial membrane potential and reactive oxygen species (ROS) production and finds a recovery mechanism for these conditions in vitrified immature oocytes. To recover from the cryoinjury, we cultured vitrified immature oocytes in milrinone containing medium for 1, 3, and 5 hours and then extended the culture for oocyte maturation. There was no difference in in vitro maturation and fertilization rate between fresh and vitrified/warmed oocytes...
August 2018: Biopreservation and Biobanking
Luciana Da Silveira Cavalcante, Jason P Acker, Jelena L Holovati
BACKGROUND: Ex vivo cold storage of red blood cells (RBCs) for transfusion has long been associated with hypothermic storage lesions. It has been proposed that liposomes can be used to mitigate hemorheological elements of RBC membrane storage lesions. This study aimed to determine the appropriate liposome treatment time and assess the effects of liposome treatment on RBC's hemorheological and metabolic profiles. MATERIALS AND METHODS: Unilamellar liposomes were synthesized to contain a bilayer of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC):cholesterol (7:3 mol%)...
August 2018: Biopreservation and Biobanking
(no author information available yet)
No abstract text is available yet for this article.
May 15, 2018: Biopreservation and Biobanking
(no author information available yet)
No abstract text is available yet for this article.
May 15, 2018: Biopreservation and Biobanking
Jim Vaught, Zisis Kozlakidis
No abstract text is available yet for this article.
May 11, 2018: Biopreservation and Biobanking
Zisis Kozlakidis, Catherine Seiler, Daniel Simeon-Dubach
No abstract text is available yet for this article.
May 10, 2018: Biopreservation and Biobanking
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"