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Biopreservation and Biobanking

Jia Luo, Hongkang Zhou, Libing Rao, Tianxiong Liu, Lian Liu, Yanliang Chen, Jianlong Wang, Tu Hu, Shiqi Liu, Dan Chen, Hui Wang, Leping Zeng, JuFang Huang
OBJECTIVES: We have explored a better method to preserve and store human medically amputated large size samples. The approach involved developing a special embalming solution and procedures for biopreservation and biostorage of a large-sized sample as a whole specimen rather than dissected small parts. Evaluation of the effect of our special embalming solution and procedures on whole human amputated extremities compared with excised small tissues was conducted. Histological and morphological techniques and elemental analyses were utilized to assess the effects of our new method using the special embalming solution...
June 11, 2018: Biopreservation and Biobanking
Jan-Eric Litton
Biobanking and BioMolecular resources Research Infrastructure (BBMRI)- European Research Infrastructure Consortium (ERIC) is the largest infrastructure launched in Europe in health research. By nature it is a distributed infrastructure, in which biological samples and data are hosted by the European Member States biobanks. As of today, BBMRI-ERIC consists of 19 European Member States and 1 international organization, the International Agency for Research on Cancer. This means that BBMRI-ERIC has a population of >500 million individuals in Europe...
May 21, 2018: Biopreservation and Biobanking
(no author information available yet)
No abstract text is available yet for this article.
May 15, 2018: Biopreservation and Biobanking
(no author information available yet)
No abstract text is available yet for this article.
May 15, 2018: Biopreservation and Biobanking
Jim Vaught, Zisis Kozlakidis
No abstract text is available yet for this article.
May 11, 2018: Biopreservation and Biobanking
Zisis Kozlakidis, Catherine Seiler, Daniel Simeon-Dubach
No abstract text is available yet for this article.
May 10, 2018: Biopreservation and Biobanking
Laura A Siminoff, Maureen Wilson-Genderson, Heather M Gardiner, Maghboeba Mosavel, Kathryn Laura Barker
Tissues from postmortem transplantation donors are a viable and productive option for genomic research. This entails obtaining authorization from the family decision makers (FDMs) of deceased donors. This study examined best practices for making such requests within the context of the Genotype-Tissue Expression (GTEx) project, a large national effort to collect reference tissues to establish a genomic biobank and database. Our study interviewed 413 FDMs about their donation experiences. We assessed FDM understanding of important consent concepts varied such as ability to withdraw tissues, the risks of donation, and return of results...
May 10, 2018: Biopreservation and Biobanking
Muhamad Abidalla, Pio F Roversi
Cryopreservation of viable cells and cell materials is being developed for biological and biopharmaceutical applications. The inhibition of ice formation during the cooling and warming phase of vitrified living biological samples is important for their survival. The tendency to form glasses (glass transition temperature, Tg ) upon cooling in the vitrification solution and the stability of the amorphous state upon warming to determine the critical cooling rate (Vccr ) and critical warming rates (Vcwr ) are evaluated...
May 10, 2018: Biopreservation and Biobanking
Rufus O Akinyemi, Kazeem Akinwande, Samuel Diala, Osi Adeleye, Abiodun Ajose, Kehinde Issa, Dorcas Owusu, Isaac Boamah, Isah Suleiman Yahaya, Abdulraheem Olayemi Jimoh, Lucius Imoh, Gregory Fakunle, Albert Akpalu, Fred Sarfo, Kolawole Wahab, Emmanuel Sanya, Lukman Owolabi, Reginald Obiako, Godwin Osaigbovo, Morenikeji Komolafe, Michael Fawale, Philip Adebayo, Paul Olowoyo, Yahaya Obiabo, Taofiki Sunmonu, Ijezie Chukwuonye, Olayemi Balogun, Basirat Adeoye, Florence Oladele, Peter Olowoniyi, Frederick Adeyemi, Arthur Lezzi, Ajibola Tunde Falayi, Michael Fasanya, Kolawole Ogunwale, Olabisi Adeola, Omolara Olomu, Olumayowa Aridegbe, Ruth Laryea, Ezinne Uvere, Moyinoluwalogo Faniyan, Ezinne Melikam, Raelle Tagge, Onoja Akpa, Joshua Akinyemi, Oyedunni Arulogun, Hemant K Tiwari, Bruce Ovbiagele, Mayowa Owolabi
Africa was previously insufficiently represented in the emerging discipline of biobanking despite commendable early efforts. However, with the Human, Heredity, and Health in Africa (H3Africa) initiative, biorepository science has been bolstered, regional biobanks are springing up, and awareness about biobanks is growing on the continent. The Stroke Investigative Research and Educational Network (SIREN) project is a transnational, multicenter, hospital and community-based study involving over 3000 cases and 3000 controls recruited from 16 sites in Ghana and Nigeria...
May 7, 2018: Biopreservation and Biobanking
William Mathieson, Nafia Guljar, Ignacio Sanchez, Manveer Sroya, Gerry A Thomas
DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissue blocks is amenable to analytical techniques, including sequencing. DNA extraction protocols are typically long and complex, often involving an overnight proteinase K digest. Automated platforms that shorten and simplify the process are therefore an attractive proposition for users wanting a faster turn-around or to process large numbers of biospecimens. It is, however, unclear whether automated extraction systems return poorer DNA yields or quality than manual extractions performed by experienced technicians...
May 3, 2018: Biopreservation and Biobanking
Keilla M Maia, Ana L P Souza, Erica C G Praxedes, Luana G P Bezerra, Andreia M Silva, Livia B Campos, Samara S J Moreira, Carlos A C Apolinário, João B F Souza, Alexandre R Silva
The influence of environmental factors in a semiarid climate on characteristics of fresh and frozen/thawed sperm collected from collared peccaries (Pecari tajacu) was assessed. Semen from 11 male collared peccaries was collected by electroejaculation during the peaks of the dry and rainy periods while rainfall indices, air temperatures, relative humidity levels, and wind speeds were measured. The number, motility, morphology, osmotic response, and membrane integrity of sperm in the collected ejaculates were assessed...
April 30, 2018: Biopreservation and Biobanking
Ma'n H Zawati, Anne Marie Tassé, Maimuna Mendy, Elodie Caboux, Michael Lang
As biobanking research in low- and middle-income countries (LMICs) continues to grow, novel legal and policy considerations have arisen. Also, while an expansive literature has developed around these issues, the views and concerns of individual researchers in these contexts have been less actively studied. These meeting notes aim to contribute to the growing literature on biobanking in LMICs by communicating a number of challenges and opportunities identified by biobank researchers themselves. Specifically, we describe concerns that emerge in consent and access policy domains...
April 18, 2018: Biopreservation and Biobanking
Kimberly White, Peixin Yang, Ling Li, Amna Farshori, Alexandre E Medina, Horst Ronald Zielke
Brain tissue from 1068 donors was analyzed for RNA quality as a function of postmortem interval (PMI) and years in storage. Approximately 83% of the cortical and cerebellar samples had an RNA integrity number (RIN) of 6 or greater, indicating their likely suitability for real-time quantitative polymerase chain reaction research. The average RIN value was independent of the PMI, up to at least 36 hours. The RNA quality for specific donated brains could not be predicted based on the PMI. Individual samples with a low PMI could have a poor RIN value, while a sample with a PMI over 36 hours may have a high RIN value...
April 2018: Biopreservation and Biobanking
Peggy Manders, Tessa M A Peters, Ariaan E Siezen, Iris A L M van Rooij, Roger Snijder, Dorine W Swinkels, Gerhard A Zielhuis
INTRODUCTION: Current guidelines for clinical biobanking have a strong focus on obtaining, handling, and storage of biospecimens. However, to allow for research tying biomarker analysis to clinical decision making, there should be more focus on collection of data on donor characteristics. Therefore, our aim was to develop a stepwise procedure to define a framework as a tool to help start the data collection process in clinical biobanking. MATERIALS AND METHODS: The Radboud Biobank (RB) is a central clinical biobanking facility designed in accordance with the standards set by the Parelsnoer Institute, a Dutch national biobank originally initiated with eight different disease cohorts...
April 2018: Biopreservation and Biobanking
Jesús García-Martínez, Daniel López Lacomba, Angel Castaño Pascual
The conservation of microorganisms is essential for their in-depth study. However, today's most widely used conservation methods, based on the use of distilled water, soil, oils, or silica, do not guarantee the stability of fungal cells, especially dermatophytes. This problem led us to evaluate the conservation capacity of a cryogenic vials system containing glass beads covered in a cryopreservant hypertonic solution as an alternative method of storage of fungal cells at -80°C. Up to 570 strains of fungi belonging to 27 different species, isolated from clinical samples, were inoculated into cryotubes containing 25 glass beads covered in a cryopreserving hypertonic solution...
April 2018: Biopreservation and Biobanking
Seyedeh Zeynab Sadr, Roya Fatehi, Saman Maroufizadeh, Christiani Andrade Amorim, Bita Ebrahimi
In vitro culture of ovarian follicles is a new technique in reproductive technology, which helps in understanding the process of folliculogenesis. The in vitro culture of follicles could be carried out using three-dimensional (3D) natural scaffolds that mimic the ovarian tissue stroma. Selection of the right matrix and culture media in these scaffolds could increase the survival and maturation of the follicles. In this work, the applicability of matrigel-alginate (MA) and fibrin-alginate (FA) 3D scaffolds for folliculogenesis was assessed...
April 2018: Biopreservation and Biobanking
Maksim V Puzanov, Liudmila B Vasilyeva, Polina V Popova, Elena N Grineva, Renata I Dmitrieva
It is widely accepted that endothelial dysfunction (ED) is a common feature and a risk factor for cardiovascular diseases and metabolic disorders. Cultures of human umbilical vein endothelial cells (HUVECs) are routinely used in cell-based models to study in vitro molecular and cellular mechanisms of development of different aspects of ED. The methods of the HUVEC extraction and expansion are well developed and standardized. However, when large collections of samples are needed for certain projects, or when samples from a rare population of patients should be collected for future experimental use, HUVEC samples should be transferred to a biobank to be saved in liquid nitrogen for a long period of time until the required collection is completed...
April 2018: Biopreservation and Biobanking
Petr Holub, Florian Kohlmayer, Fabian Prasser, Michaela Th Mayrhofer, Irene Schlünder, Gillian M Martin, Sara Casati, Lefteris Koumakis, Andrea Wutte, Łukasz Kozera, Dominik Strapagiel, Gabriele Anton, Gianluigi Zanetti, Osman Ugur Sezerman, Maimuna Mendy, Dalibor Valík, Marialuisa Lavitrano, Georges Dagher, Kurt Zatloukal, GertJan B van Ommen, Jan-Eric Litton
The known challenge of underutilization of data and biological material from biorepositories as potential resources for medical research has been the focus of discussion for over a decade. Recently developed guidelines for improved data availability and reusability-entitled FAIR Principles (Findability, Accessibility, Interoperability, and Reusability)-are likely to address only parts of the problem. In this article, we argue that biological material and data should be viewed as a unified resource. This approach would facilitate access to complete provenance information, which is a prerequisite for reproducibility and meaningful integration of the data...
April 2018: Biopreservation and Biobanking
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