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Biopreservation and Biobanking

William E Grizzle, Katherine C Sexton
Many classic biobanks collect more human tissues than they distribute, leading to increased inventories, unnecessary storage, increased expenses, and reduced chargeback income. This situation is a result of biobanks operating without well-defined goals, having incorrect views of the potential number of investigators who will utilize specimens, and collection of biospecimens without adequately considering the need for specific tissues by investigators. These deficiencies frequently lead to unrealistic plans for biospecimen utilization and biobanks that are larger than necessary...
December 1, 2018: Biopreservation and Biobanking
Iiro Hämäläinen, Outi Törnwall, Birgit Simell, Kurt Zatloukal, Markus Perola, Gert-Jan B van Ommen
Public-private partnerships (PPP) are an efficient means to advance scientific discoveries and boost the medical innovations needed to improve precision medicine. The increasing number and novel nature of such collaborations is keeping the biomedical field in a constant flux. Here we provide an update on PPP development involving academic biobanks in the BBMRI community (the European Biobanking and BioMolecular Resources Research Infrastructure) and report the views on PPP of 20 key players from this field...
November 30, 2018: Biopreservation and Biobanking
Chunrong Lv, Guoquan Wu, Qionghua Hong, Guobo Quan
The cryotolerance of farm animal spermatozoa varies according to their specific features, such as size, shape, and lipid composition. Thus, it is impossible to develop a standardized freezing procedure for different kinds of livestock species. The establishment of an efficient semen cryopreservation procedure will facilitate long-term conservation of small ruminant genetic resources and extension of artificial insemination in daily production. Different from sheep, goat seminal plasma contains a phospholipase, which can affect spermatozoa viability through interaction with milk or egg yolk...
November 30, 2018: Biopreservation and Biobanking
Ariadna Corral, Reyes López, Marcin Balcerzyk, Ángel Parrado-Gallego, Isabel Fernández-Gómez, Alberto Olmo, Ramón Risco
One of the main problems in the cryopreservation of biological samples is the formation of ice and the consequent mechanical damage to cells and tissues, due to the crystalline structure of ice and its associated mechanical damage. It is necessary to detect this deleterious formation of ice, especially in tissues and organs, because of their large volume and the complexity of their vascular system in the case of bulky organs. In this work, we propose the use of X-ray Computed Tomography (CT) to detect this ice formation inside tissues and organs...
November 29, 2018: Biopreservation and Biobanking
Min Wang, Xiaoli Ji, Bingjie Wang, Qian Li, Junmei Zhou
A major concern in biomedical research is the quality of biological samples. RNAlater is a stabilizer, which was originally developed for RNA preservation in fresh tissues and is important for collection and transportation. However, this reagent lacks a comprehensive and systematic evaluation of its preservative effect on different mammalian tissues under consistent experimental conditions. In this study, we collected liver, kidney, testis, brain, and colon tissues from mice and divided the samples into the following respective groups: fresh, RNAlater preserved, and liquid nitrogen snap frozen...
November 28, 2018: Biopreservation and Biobanking
Jianping Sun, Mengdan Gao, Kang Li, Ling Qin, Huanqin Sun, Guifang Qiao, Yan Zhao, Yonghong Zhang
BACKGROUND: Peripheral blood mononuclear cells (PBMCs) count among the most important samples in a biobank, and the quality of cryopreserved PBMCs is crucial for further research. This study evaluated the quality of PBMCs recovered from the Beijing Capital Medical University Hepatitis/AIDS Biobank after 2-11 years of cryopreservation. MATERIALS AND METHODS: A total of 87 PBMC samples with different cryopreservation times (2006, 2007, 2013, and 2015) were thawed, and the cell number and cell viability were determined by acridine orange/propidium iodide staining...
November 27, 2018: Biopreservation and Biobanking
Jufang Huang, Liangliang Ruan, Rongxing Gan
No abstract text is available yet for this article.
November 21, 2018: Biopreservation and Biobanking
Xia Zhang, Qiu-Yue Han, Zhang-Sheng Zhao, Jian-Gang Zhang, Wen-Jun Zhou, Aifen Lin
BACKGROUND: Molecular research is increasingly dependent on high-quality biobanked biospecimens. Preanalytical variables in tissue processing and preservation may influence the RNA quality and research results. Hence, the effect of long-term storage and clinicopathological parameters on RNA quality needs to be elucidated. METHODS: Ninety gastric cancer tissue samples were collected and fresh-frozen in a -80°C freezer for 12 years (2006-2017). The histology was assessed and RNA integrity number (RIN) was detected by an Agilent 2100 Bioanalyzer...
November 20, 2018: Biopreservation and Biobanking
William E Grizzle, Katherine C Sexton, Diane McGarvey, Zachery V Menchhofen, Virginia LiVolsi
Prospective collection is a model through which biospecimens are provided for research. Using this model, biospecimens are collected based on real-time requests from the research community instead of being collected based on the prediction of future requests. We describe the lessons learned by two bioresources that have operated successfully using a prospective model for over three decades. Our goal is to improve other bioresources by increasing utilization of biospecimens that honor consented donors who provide biospecimens to the research community; this provides strong evidence of stewardship of the public trust...
November 20, 2018: Biopreservation and Biobanking
Ricássio S Barberino, José Roberto V Silva, José Ricardo Figueiredo, Maria Helena T Matos
Short-term storage of ovaries during their transport from the collection sites to the specialized laboratories allows the recovery of thousands of oocytes from females of high genetic value, endangered species, and companion or transgenic animals, which sometimes die unexpectedly in the field, or are ovariectomized for medical reasons. Therefore, several studies have been performed to find ideal protocols to preserve oocyte viability during ovarian tissue transport, thus ensuring the success of techniques that are performed after the storage, such as cryopreservation and/or in vitro follicle culture...
November 10, 2018: Biopreservation and Biobanking
Marianne K Henderson, Kirstin Goldring, Daniel Simeon-Dubach
Quality specimens from biobanks are key resources to support reproducible research. Sustaining biobanks requires robust management. We recently published a pilot survey that indicated that over half the participating biobanks had business plans in place and another third were working on business planning. While the results provided a clue to the status of business planning in biobanking, it was concluded that a longer and more in-depth survey and analysis were required. In April 2017, an extended survey was distributed worldwide in English, French, Chinese, German, and Spanish, through multiple channels...
November 9, 2018: Biopreservation and Biobanking
Bénedith Oben, Charlotte Cosemans, Ingrid Arijs, Loes Linsen, Annick Daniëls, Jeroen Declercq, Brigitte Maes, Kimberly Vanhees, Guy Froyen, Jean-Luc Rummens
Biobanking is increasingly important in studying complex heterogeneous diseases. Therefore, it is essential to ensure the sample quality after long-term storage for reliable downstream analyses. The Clinical Biobank of the Jessa Hospital and the University Biobank Limburg (UBiLim) hold a continuously growing collection of hematological samples, including May-Grünwald-Giemsa (MGG)- and Perls' Prussian Blue (PPB)-stained bone marrow (BM) smears, stored at room temperature (RT) for up to 20 years. In this study, we investigated the effect of short- and long-term storage on the quality of DNA and RNA extracted from these BM smears to assess their fitness-for-purpose in downstream molecular applications, including agarose gel electrophoresis, bio-analyzer analysis, quantitative polymerase chain reaction (qPCR), and targeted next-generation sequencing (NGS)...
November 9, 2018: Biopreservation and Biobanking
Qiyuan Li, Xian Wang, Xue Li, Xuheng He, Qian Wan, Jiefang Yin, Jianbo Sun, Xiaoping Yang, Qiaohong Chen, Xinyuan Miao
Blood is a biological fluid that contains multiple blood fraction and cellular components. High-quality blood specimens are essential prerequisites for various downstream applications such as molecular epidemiology studies, genomics, and proteomics studies. Currently, protocols and research publications concerning the collection, handling, preservation, and stability of blood or blood fractions are constantly emerging. Moreover, standardized guidelines are a requirement for biorepositories to tightly control preanalytical variables originating from these procedures and obtain high-quality blood specimen for downstream analyses...
October 31, 2018: Biopreservation and Biobanking
Zhang Heng, Liangliang Ruan, Rongxing Gan
Leukocytes function as central effectors in innate immunity (such as phagocytosis) as well as adaptive immunity (e.g., antigen-dependent T cell activation), and serve as an important resource in the fields of translational medicine, precision medicine, and cell therapy. Isolation of leukocytes from whole blood is necessary for high-quality RNA and downstream research. This process is susceptible to the variability of many factors, such as blood collection, isolation reagents, and extraction methods. In this study, three methods were applied for leukocytes separation, followed by RNA extraction and quality testing to evaluate the methods...
October 31, 2018: Biopreservation and Biobanking
David G Nohle, Randal L Mandt, Marta E Couce, Anil V Parwani, Leona W Ayers
BACKGROUND: The Cooperative Human Tissue Network, Midwestern Division, is a National Cancer Institute-funded program that provides quality research biospecimens to qualified investigators. Consented human tissues are procured according to researcher specifications for weight (size) and preservation type; weights of samples in significant demand and limited supply are negotiated. Weights of procured tissues are entered into a dedicated biospecimen database. This study seeks to provide guidance for acceptable tissue weights for researchers...
October 31, 2018: Biopreservation and Biobanking
Shanshan Qiu, Yunhai Song, Jing Wang, Pingping Gao, Jing Chen, Ru Chen, Nan Bao, Shijian Liu
OBJECTIVE: To examine the factors that may influence Chinese parent's willingness to donate their children's biospecimens for use in pediatric research. STUDY DESIGN: Parents or caregivers of the patients in the neurosurgery ward, oncological surgery ward, and internal medical wards at Shanghai Children's Medical Center were recruited during the period of March 1, 2016 to July 8, 2018. The questionnaire included the willingness to provide consent for donating their children's clinical biospecimens, their attitudes toward and motivations for donating their children's clinical biospecimens, opinions of contributing specimens, and an ethical consideration for their children's future willingness to donate biospecimens...
October 26, 2018: Biopreservation and Biobanking
Helmuth Haslacher, Marlene Gerner, Philipp Hofer, Andreas Jurkowitsch, Johannes Hainfellner, Renate Kain, Oswald F Wagner, Thomas Perkmann
BACKGROUND AND AIM: It is increasingly recognized that biomedical research has serious reproducibility issues, which could be overcome at least in part by standardized processing of biomaterials. Therefore, professional biobanks have emerged, positively influencing sample and data quality. However, quantitative data about a biobank's contribution to published results are still hard to find, although they could serve as valuable benchmark figures for the community. We therefore aimed to report usage data from the MedUni Wien Biobank facility regarding its prospective fluid cohorts...
October 18, 2018: Biopreservation and Biobanking
Lucie Gavin-Plagne, Loris Commin, Pierre Bruyère, Samuel Buff, Thierry Joly
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility...
October 16, 2018: Biopreservation and Biobanking
Andrey Nikolayevich Khudyakov, Larisa Georgievna Kuleshova, Oksana Olegovna Zaitseva, Marta Igorevna Sergushkina, Konstantin Aleksandrovich Vetoshkin, Tatyana Vitalyevna Polezhaeva
The ability of various pectin polysaccharides to modify the morphological structure of ice during the phase transitions from water to ice was studied. Pectins were isolated from Sosnowsky's hogweed Heracleum sosnowskyi Manden (heracleuman-N6HS), tansy Tanacetum vulgare L. (tanacetan-N7TVF), and Rauwolfia serpentina Benth callus (rauwolfian-N8RS). Pectins were isolated by multistep extraction. The effect of pectins was assessed using osmometry, thermographic analysis, and cryomicroscopy. A concentrate of leukocytes was used as the sample for the subsequent freezing step...
October 9, 2018: Biopreservation and Biobanking
Yanhong Liu, Hong Gao, Yue Hu, Jie Ding, Meiling Ge, Qing Ye
AIM: To ensure that sample quality meets the requirements of experimental research, the gynecology and obstetrics biobank of the Nanjing Drum Tower hospital designed different quality control methods for relevant types of samples. A range of quality control procedures has been formulated. METHODS: The sample types were frozen tissue, paraffin-embedded tissue, optimal cutting temperature (OCT)-embedded tissue, plasma, buffy coat, serum, blood clots, and urine. Different categories of samples from a random selection of 1% of cases were analyzed for quality control experiments: (i) frozen tissue, buffy coat, and blood clots: RNA and DNA were extracted and the concentration, purity, and integrity were determined; (ii) paraffin-embedded tissue: morphological observations were made after hematoxylin-eosin staining and immunohistochemical detection of β-actin or CD10; (iii) OCT-embedded tissue: hematoxylin-eosin staining and immunofluorescence detection of β-actin; and (iv) frozen tissue samples derived from different organs of 18 fetal autopsy specimens with different cold ischemia times (CITs), 0-12 hours, 12-18 hours, 18-24 hours, and 24-48 hours, were chosen to study RNA quality...
October 6, 2018: Biopreservation and Biobanking
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