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Biopreservation and Biobanking

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https://www.readbyqxmd.com/read/28594242/attitudes-and-perceptions-of-cancer-patients-toward-biospecimen-donation-for-cancer-research-a-cross-sectional-survey-among-chinese-cancer-patients
#1
Na He, Yan Guo, Min He, Wanmin Qiang, Haixin Li
BACKGROUND: High-quality biospecimen collection from consented patients is crucial for cancer research activities. Patients' attitudes and willingness toward specimen donation influence high-quality biospecimen collection for cancer research activities. METHODS: We carried out a cross-sectional study among randomly selected patients from 11 cancer departments of Tianjin Medical University Cancer Institute and Hospital between August 2014 and August 2015. A total of 784 patients were included to complete a 30-item self-administered survey...
June 8, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28586236/inspecting-targeted-deep-sequencing-of-whole-genome-amplified-dna-versus-fresh-dna-for-somatic-mutation-detection-a-genetic-study-in-myelodysplastic-syndrome-patients
#2
Laura Palomo, Francisco Fuster-Tormo, Daniel Alvira, Vera Ademà, María Pilar Armengol, Paula Gómez-Marzo, Nuri de Haro, Mar Mallo, Blanca Xicoy, Lurdes Zamora, Francesc Solé
Whole genome amplification (WGA) has become an invaluable method for preserving limited samples of precious stock material and has been used during the past years as an alternative tool to increase the amount of DNA before library preparation for next-generation sequencing. Myelodysplastic syndromes (MDS) are a group of clonal hematopoietic stem cell disorders characterized by presenting somatic mutations in several myeloid-related genes. In this work, targeted deep sequencing has been performed on four paired fresh DNA and WGA DNA samples from bone marrow of MDS patients, to assess the feasibility of using WGA DNA for detecting somatic mutations...
June 6, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28574760/targeting-future-customers-an-introductory-biobanking-course-for-undergraduate-students-of-life-sciences
#3
Ahmed Samir Abdelhafiz, Merhan Ahmed Fouda, Shaimaa Ibrahim El-Jaafary, Maysa Ibrahim Farghly, Mazen Salem, Ahmed Tammam, Hala Gabr
INTRODUCTION: Biobanking is a relatively new concept in the Arab region. Targeting different stakeholders to introduce the concept of biobanking and develop an acceptance of it among them is important for the growth of biobanking in the region. Undergraduate students of life sciences represent an important segment of stakeholders, since they constitute potential future biobank customers. Limited funding, lack of awareness of the existence of the term "biobanking" itself among these students, and questions regarding best marketing strategies presented challenges to planning for the most effective message delivery to this target group...
June 2, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28541714/a-multivariate-evaluation-of-factors-affecting-the-quality-of-freshly-frozen-tissue-specimens
#4
Tong-Hong Wang, Chin-Chuan Chen, Kung-Hao Liang, Chi-Yuan Chen, Wen-Yu Chuang, Shir-Hwa Ueng, Pao-Hsien Chu, Chung-Guei Huang, Tse-Ching Chen, Chuen Hsueh
Well-prepared and preserved freshly frozen specimens are indispensable materials for clinical studies. To manage specimen quality and to understand the factors potentially affecting specimen quality during preservation processes, we analyzed the quality of RNA and genomic DNA of various tissues collected between 2002 and 2011 in Linkou Chang Gung Memorial Hospital, Taiwan. During this period, a total of 1059 freshly frozen specimens from eight major cancer categories were examined. It was found that preservation duration, organ origin, and tissue type could all influence the quality of RNA samples...
May 25, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28441039/high-throughput-processing-to-preserve-viable-cells-a-precision-medicine-initiative-cohort-program-workshop
#5
Rebekah S Rasooly, Daniel R Gossett, Marianne K Henderson, Allison Hubel, Stephen N Thibodeau
Conventionally, biobanks supporting clinical research studies have preserved serum, plasma, urine, saliva, a variety of tissue types, and stool. With the emergence of increasingly sophisticated technologies for analyzing single cells, there is growing interest in preserving viable blood cells for future functional studies. The new All of Us Research Program (formerly the Precision Medicine Initiative Cohort Program) biobank plans to house samples from a million or more individuals as part of a cohort with rich phenotypic data and longitudinal follow-up ( www...
April 25, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28426236/rna-extracted-from-formalin-fixed-paraffin-embedded-renal-biopsy-biospecimens-an-evaluation-of-alternative-extraction-kits-and-the-effects-of-storage-time
#6
Yinghui Lu, Mingchao Zhang, Qi Jiang, Ru Yin, Ping Zhu, Hongtian Wang, Zhihong Liu, Chunxia Zheng
No abstract text is available yet for this article.
April 20, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28394173/sheep-isolated-secondary-follicles-are-able-to-produce-metaphase-ii-oocytes-after-vitrification-and-long-term-in-vitro-growth
#7
Franciele Osmarini Lunardi, Francisco Leo Nascimento de Aguiar, Livia Brunetti Apolloni, Ana Beatriz Graça Duarte, Naiza Arcângela Ribeiro de Sá, Érica Suzanne Soares Leal, Antonia Debora Sales, Carlos Henrique Lobo, Cláudio Cabral Campello, Johan Smitz, Gary Allen Apgar, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues
The vitrification of preantral follicles followed by in vitro growth (IVG) could be valuable to produce fertilizable oocytes. However, the meiotic resumption rates of oocytes cultured from vitrified secondary follicles (SF) have been reported as suboptimal. This study aimed to verify two base media (alpha modification of minimum essential medium, α-MEM, and tissue culture medium 199, TCM199) on vitrified SF regarding different requirements during IVG. Sheep ovarian fragments were divided in six groups: (1) Fresh groups (Control α-MEM and TCM199): SF without vitrification; (2) Follicle-Vitrified (Follicle-Vit α-MEM and TCM199): SF vitrified after isolation; and (3) Tissue-Vitrified (Tissue-Vit α-MEM and TCM199): SF vitrified enclosed in ovarian fragments and, subsequently, isolated...
April 10, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28388226/a-review-of-research-grade-human-induced-pluripotent-stem-cells-qualification-and-biobanking-processes
#8
Aikaterini Ntai, Simona Baronchelli, Alberto La Spada, Anna Moles, Alessandro Guffanti, Pasquale De Blasio, Ida Biunno
Human induced pluripotent stem cell (hiPSC) biobanks are invaluable resources for basic and clinical research, since they provide a sustainable supply of accessible cell lines that meet high quality and safety standards. hiPSCs are particularly useful for understanding disease mechanisms, creating cell models for drug development, and generating novel clinical therapies. For clinical applications and drug discovery, it is fundamental that the acquired pluripotent cell lines never touch animal-derived products nor xenogeneic reagents (Good Manufacturing Practice-grade); whereas for research grade, it is sufficient to operate under Good Laboratory Practice conditions...
April 7, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28380303/conception-and-implementation-of-an-austrian-biobank-directory-integration-framework
#9
Philipp Hofer-Picout, Horst Pichler, Johann Eder, Sabrina B Neururer, Heimo Müller, Robert Reihs, Petr Holub, Thomas Insam, Georg Goebel
INTRODUCTION: Sample collections and data are hosted within different biobanks at diverse institutions across Europe. Our data integration framework aims at incorporating data about sample collections from different biobanks into a common research infrastructure, facilitating researchers' abilities to obtain high-quality samples to conduct their research. The resulting information must be locally gathered and distributed to searchable higher level information biobank directories to maximize the visibility on the national and European levels...
April 5, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28574775/the-2017-isber-annual-meeting-exhibits-due-north-aligning-biobanking-practice-with-evolving-evidence-and-innovation
#10
Alison Parry-Jones, Monique Albert
No abstract text is available yet for this article.
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28530442/midyear-report-continuing-the-anniversary-celebration
#11
Jim Vaught
No abstract text is available yet for this article.
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28471704/the-international-society-for-biological-and-environmental-repositories-presents-abstracts-from-its-annual-meeting-due-north-aligning-biobanking-practice-with-evolving-evidence-and-innovation-may-9-12-2017-toronto-canada
#12
https://www.readbyqxmd.com/read/28170288/influence-of-freeze-thaw-cycles-on-rna-integrity-of-gastrointestinal-cancer-and-matched-adjacent-tissues
#13
Ying Hu, Haibo Han, Yixue Wang, Lijie Song, Xiaojing Cheng, Xiaofang Xing, Bin Dong, Xiaohong Wang, Meng Chen, Lianhai Zhang, Jiafu Ji
BACKGROUND: Comparative analysis of RNA expression profiles between cancer and adjacent noncancerous tissues is an important part of cancer research. High-quality RNA is essential for consistent, reliable results, especially for identification of cancer biomarkers. However, the impact of freeze-thaw cycles on the quality of RNA both in gastrointestinal cancer and paired adjacent tissues is still unclear. AIM: To investigate the influence of freeze-thaw cycles on RNA integrity and overall histomorphology of gastrointestinal cancer and paired adjacent noncancerous tissues...
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28103080/organization-of-bbmri-pl-the-polish-biobanking-network
#14
Małgorzata Witoń, Dominik Strapagiel, Joanna Gleńska-Olender, Anna Chróścicka, Katarzyna Ferdyn, Jarosław Skokowski, Leszek Kalinowski, Jakub Pawlikowski, Błażej Marciniak, Markus Pasterk, Agnieszka Matera-Witkiewicz, Łukasz Kozera
In Poland storage of human biological samples takes place at most universities and scientific institutions conducting research projects in the field of biomedicine. The First International Biobanking Conference organized by the Ministry of Science and Higher Education in 2014 shed a light on the situation of Polish biobanking infrastructures. The country has around 40 large biorepositories, which store unique biological material such as whole brains, muscle fibers from patients with rare diseases, as well as thousands of samples from patients with lifestyle diseases...
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28080144/a-survey-of-the-current-situation-of-clinical-biobanks-in-china
#15
Haiyan Li, Mingyu Ni, Peng Wang, Xiaomin Wang
The development of biomedical research urgently needs the support of a large number of high-quality clinical biospecimens. Therefore, human biobanks at different levels have been established successively in China and other countries at a significantly increasing pace in recent years. To better understand the general current state of clinical biobanks in China, we surveyed 42 clinical biobanks based in hospitals and collected information involving their management systems, sharing mechanisms, quality control systems, and informational management systems using closed questionnaire methods...
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28080142/whole-genome-amplification-provides-suitable-control-dna-for-use-in-dna-barcoding-applications
#16
Danielle A Bourque, Amanda M Naaum, Dan L Distel, Robert H Hanner
No abstract text is available yet for this article.
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28072924/cryopreservation-of-carp-cyprinus-carpio-l-sperm-impact-of-seeding-and-freezing-rates-on-post-thaw-outputs
#17
Sergii Boryshpolets, Denisa Sochorová, Marek Rodina, Otomar Linhart, Borys Dzyuba
In the present study, we examined various freezing protocols, effects of controlled seeding, and changes in cooling rate and determined the endpoint (temperature at which sample could be plugged into liquid nitrogen (LN) without visible effect on survival rate after thawing) to reveal the relative importance of each different stage of cooling on freezing success during cryobanking of carp sperm. Sperm samples from different individual carp males were frozen in 0.5 mL straws by conventional freezing. Cooling rates were determined by monitoring the sample's internal temperature...
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28051325/measurement-of-thermal-conductivities-of-two-cryoprotective-agent-solutions-for-vitreous-cryopreservation-of-organs-at-the-temperature-range-of-77%C3%A2-k-300%C3%A2-k-using-a-thermal-sensor-made-of-microscale-enamel-copper-wire
#18
Yufang Li, Gang Zhao, S M Chapal Hossain, Fazil Panhwar, Wenyu Sun, Fei Kong, Chuanbao Zang, Zhendong Jiang
Biobanking of organs by cryopreservation is an enabling technology for organ transplantation. Compared with the conventional slow freezing method, vitreous cryopreservation has been regarded to be a more promising approach for long-term storage of organs. The major challenges to vitrification are devitrification and recrystallization during the warming process, and high concentrations of cryoprotective agents (CPAs) induced metabolic and osmotic injuries. For a theoretical model based optimization of vitrification, thermal properties of CPA solutions are indispensable...
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28005396/the-effect-of-canthaxanthin-on-the-quality-of-frozen-ram-spermatozoa
#19
Helder M Souza, Lúcia C P Arruda, Millena M Monteiro, Igor H A V Nery, Robespierre A J Araújo Silva, André M Batista, Maria Madalena Pessoa Guerra
The addition of antioxidants to semen cryopreservation extenders has been employed for combating oxidative damage. This work aimed to evaluate the addition of carotenoid canthaxanthin to a cryopreservation extender of ram semen. Three breeder rams were used and, after semen collection, with 48-hour intervals between collection, the samples were included in the pool formation (n = 6). The experimental groups comprised 0 (control), 0.1, 1, 10, and 25 μM of canthaxanthin. After thawing (37°C/30 s) and incubation at 37°C for 2 hours, semen aliquots from each group were evaluated for sperm kinetics (CASA), the integrity of the plasma and acrosomal membranes (iPAM), intracellular reactive oxygen species (ROS) production, and lipid peroxidation (LPO) by flow cytometry associated with the image...
June 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/27992240/quality-matters-2016-annual-conference-of-the-national-infrastructures-for-biobanking
#20
Marika Doucet, Karl Friedrich Becker, Jens Björkman, Jacques Bonnet, Bruno Clément, Maria-Grazia Daidone, Charles Duyckaerts, Gilles Erb, Helmuth Haslacher, Paul Hofman, Berthold Huppertz, Christophe Junot, Joakim Lundeberg, Andres Metspalu, Marialuisa Lavitrano, Jan-Eric Litton, Helen M Moore, Manuel Morente, Ben-Youssef Naimi, Uwe Oelmueller, Bill Ollier, Barbara Parodi, Liangliang Ruan, Giorgio Stanta, Paola Turano, Jim Vaught, Peter Watson, H-Erich Wichmann, Martin Yuille, Myriam Zaomi, Kurt Zatloukal, Georges Dagher
No abstract text is available yet for this article.
June 2017: Biopreservation and Biobanking
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