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Biopreservation and Biobanking

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https://www.readbyqxmd.com/read/28426236/rna-extracted-from-formalin-fixed-paraffin-embedded-renal-biopsy-biospecimens-an-evaluation-of-alternative-extraction-kits-and-the-effects-of-storage-time
#1
Yinghui Lu, Mingchao Zhang, Qi Jiang, Ru Yin, Ping Zhu, Hongtian Wang, Zhihong Liu, Chunxia Zheng
No abstract text is available yet for this article.
April 20, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28394173/sheep-isolated-secondary-follicles-are-able-to-produce-metaphase-ii-oocytes-after-vitrification-and-long-term-in-vitro-growth
#2
Franciele Osmarini Lunardi, Francisco Leo Nascimento de Aguiar, Livia Brunetti Apolloni, Ana Beatriz Graça Duarte, Naiza Arcângela Ribeiro de Sá, Érica Suzanne Soares Leal, Antonia Debora Sales, Carlos Henrique Lobo, Cláudio Cabral Campello, Johan Smitz, Gary Allen Apgar, José Ricardo de Figueiredo, Ana Paula Ribeiro Rodrigues
The vitrification of preantral follicles followed by in vitro growth (IVG) could be valuable to produce fertilizable oocytes. However, the meiotic resumption rates of oocytes cultured from vitrified secondary follicles (SF) have been reported as suboptimal. This study aimed to verify two base media (alpha modification of minimum essential medium, α-MEM, and tissue culture medium 199, TCM199) on vitrified SF regarding different requirements during IVG. Sheep ovarian fragments were divided in six groups: (1) Fresh groups (Control α-MEM and TCM199): SF without vitrification; (2) Follicle-Vitrified (Follicle-Vit α-MEM and TCM199): SF vitrified after isolation; and (3) Tissue-Vitrified (Tissue-Vit α-MEM and TCM199): SF vitrified enclosed in ovarian fragments and, subsequently, isolated...
April 10, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28388226/a-review-of-research-grade-human-induced-pluripotent-stem-cells-qualification-and-biobanking-processes
#3
Aikaterini Ntai, Simona Baronchelli, Alberto La Spada, Anna Moles, Alessandro Guffanti, Pasquale De Blasio, Ida Biunno
Human induced pluripotent stem cell (hiPSC) biobanks are invaluable resources for basic and clinical research, since they provide a sustainable supply of accessible cell lines that meet high quality and safety standards. hiPSCs are particularly useful for understanding disease mechanisms, creating cell models for drug development, and generating novel clinical therapies. For clinical applications and drug discovery, it is fundamental that the acquired pluripotent cell lines never touch animal-derived products nor xenogeneic reagents (Good Manufacturing Practice-grade); whereas for research grade, it is sufficient to operate under Good Laboratory Practice conditions...
April 7, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28380303/conception-and-implementation-of-an-austrian-biobank-directory-integration-framework
#4
Philipp Hofer-Picout, Horst Pichler, Johann Eder, Sabrina B Neururer, Heimo Müller, Robert Reihs, Petr Holub, Thomas Insam, Georg Goebel
INTRODUCTION: Sample collections and data are hosted within different biobanks at diverse institutions across Europe. Our data integration framework aims at incorporating data about sample collections from different biobanks into a common research infrastructure, facilitating researchers' abilities to obtain high-quality samples to conduct their research. The resulting information must be locally gathered and distributed to searchable higher level information biobank directories to maximize the visibility on the national and European levels...
April 5, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28328240/insights-on-cryopreserved-sheep-fibroblasts-by-cryomicroscopy-and-gene-expression-analysis
#5
Antonella Idda, Daniela Bebbere, Giuseppina Corona, Laura Masala, Elisa Casula, Alberto Cincotti, Sergio Ledda
Cryopreservation includes a set of techniques aimed at storing biological samples and preserving their biochemical and functional features without any significant alterations. This study set out to investigate the effects induced by cryopreservation on cultured sheepskin fibroblasts (CSSF) through cryomicroscopy and gene expression analysis after subsequent in vitro culture. CSSF cells were cryopreserved in a cryomicroscope (CM) or in a straw programmable freezer (SPF) using a similar thermal profile (cooling rate -5°C/min to -120°C, then -150°C/min to -196°C)...
March 22, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28398809/tagging-of-test-tubes-with-electronic-p-chips-for-use-in-biorepositories
#6
Wlodek Mandecki, Wesley M Kopacka, Ziye Qian, Von Ertwine, Katie Gedzberg, Maryann Gruda, David Reinhardt, Efrain Rodriguez
A system has been developed to electronically tag and track test tubes used in biorepositories. The system is based on a light-activated microtransponder, also known as a "p-Chip." One of the pressing problems with storing and retrieving biological samples at low temperatures is the difficulty of reliably reading the identification (ID) number that links each storage tube with the database containing sample details. Commonly used barcodes are not always reliable at low temperatures because of poor adhesion of the label to the test tube and problems with reading under conditions of frost and ice accumulation...
February 23, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28398808/isolation-methods-of-peripheral-blood-mononuclear-cells-in-spanish-biobanks-an-overview
#7
Irene Consuegra, Clara Rodríguez-Aierbe, Inés Santiuste, Anna Bosch, Rosario Martínez-Marín, Ma Antonia Fortuto, Tatiana Díaz, Salvador Martí, Ma Ángeles Muñoz-Fernández
The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.
February 22, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28253021/the-need-for-research-infrastructures-a-narrative-review-of-large-scale-research-infrastructures-in-biobanking
#8
Anthony Larsson
BACKGROUND: Distributed Research Infrastructures are gaining political traction in Europe to facilitate scientific research. This development has gained particular momentum in the area of biobanking where cross-national attempts have been made toward harmonizing the biobanking standards across the European Union through the establishment of the organization BBMRI (BioBanking and Molecular Resource Infrastructure). BBMRI exists as separate national nodes across several European countries, although Sweden took on a pioneering role in its early stages...
February 21, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28170288/influence-of-freeze-thaw-cycles-on-rna-integrity-of-gastrointestinal-cancer-and-matched-adjacent-tissues
#9
Ying Hu, Haibo Han, Yixue Wang, Lijie Song, Xiaojing Cheng, Xiaofang Xing, Bin Dong, Xiaohong Wang, Meng Chen, Lianhai Zhang, Jiafu Ji
BACKGROUND: Comparative analysis of RNA expression profiles between cancer and adjacent noncancerous tissues is an important part of cancer research. High-quality RNA is essential for consistent, reliable results, especially for identification of cancer biomarkers. However, the impact of freeze-thaw cycles on the quality of RNA both in gastrointestinal cancer and paired adjacent tissues is still unclear. AIM: To investigate the influence of freeze-thaw cycles on RNA integrity and overall histomorphology of gastrointestinal cancer and paired adjacent noncancerous tissues...
February 7, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28157384/evaluation-of-the-analytical-and-clinical-concordance-of-25-hydroxyvitamin-d-levels-in-dried-blood-spots-dried-serum-spots-and-serum-as-potential-biorepository-specimens
#10
Gellert Karvaly, Györgyi Molnár-Világos, Krisztián Kovács, Katalin Mészáros, Attila Patócs, Barna Vásárhelyi
CONTEXT: The promising perspective of storing dried blood spot (DBS) for evaluating 25-hydroxyvitamin D (25OHD) levels is increasingly being realized. While strong correlations have been demonstrated between 25OHD levels measured in DBS and in systemic serum samples in earlier works, the clinical concordance of the assay results has not been evaluated. Moreover, the utility of dried serum spot (DSS), a highly suitable matrix for sample archiving, has not been investigated in this respect...
February 3, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28151003/engaging-diverse-stakeholders-to-inform-biobank-governance
#11
Marie Murphy, Sarah B Garrett, Elizabeth Boyd, Sarah Dry, Daniel Dohan
No abstract text is available yet for this article.
February 2, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28398834/dry-preservation-of-spermatozoa-considerations-for-different-species
#12
Jennifer Patrick, Pierre Comizzoli, Gloria Elliott
The current gold standard for sperm preservation is storage at cryogenic temperatures. Dry preservation is an attractive alternative, eliminating the need for ultralow temperatures, reducing storage maintenance costs, and providing logistical flexibility for shipping. Many seeds and anhydrobiotic organisms are able to survive extended periods in a dry state through the accumulation of intracellular sugars and other osmolytes and are capable of returning to normal physiology postrehydration. Using techniques inspired by nature's adaptations, attempts have been made to dehydrate and dry preserve spermatozoa from a variety of species...
April 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28375760/ethical-legal-and-social-issues-of-biobanking-past-present-and-future
#13
Marianna J Bledsoe
The past 15 years has seen considerable changes in the research environment. These changes include the development of new sophisticated genetic and genomic technologies, a proliferation of databases containing large amount of genotypic and phenotypic data, and wide-spread data sharing among many institutions, nationally and internationally. These changes have raised new questions regarding how best to protect the participants of biobanking research. In response to these questions, best practices for addressing the legal, ethical, and social issues of biobanking have been developed...
April 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28375759/baobab-laboratory-information-management-system-development-of-an-open-source-laboratory-information-management-system-for-biobanking
#14
Hocine Bendou, Lunga Sizani, Tim Reid, Carmen Swanepoel, Toluwaleke Ademuyiwa, Roxana Merino-Martinez, Heimo Meuller, Akin Abayomi, Alan Christoffels
A laboratory information management system (LIMS) is central to the informatics infrastructure that underlies biobanking activities. To date, a wide range of commercial and open-source LIMSs are available and the decision to opt for one LIMS over another is often influenced by the needs of the biobank clients and researchers, as well as available financial resources. The Baobab LIMS was developed by customizing the Bika LIMS software ( www.bikalims.org ) to meet the requirements of biobanking best practices...
April 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28353358/financial-sustainability-of-biobanks-from-theory-to-practice
#15
Moderator Martin Yuille, Participants Irwin Feller, Luke Georghiou, Philippe Laredo, Eric W Welch
No abstract text is available yet for this article.
April 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28346801/our-15-th-anniversary-issue
#16
Jim Vaught
No abstract text is available yet for this article.
April 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28338350/consensus-driven-development-of-a-terminology-for-biobanking-the-duke-experience
#17
Helena Ellis, Mary-Beth Joshi, Aenoch J Lynn, Anita Walden
Biobanking at Duke University has existed for decades and has grown over time in silos and based on specialized needs, as is true with most biomedical research centers. These silos developed informatics systems to support their own individual requirements, with no regard for semantic or syntactic interoperability. Duke undertook an initiative to implement an enterprise-wide biobanking information system to serve its many diverse biobanking entities. A significant part of this initiative was the development of a common terminology for use in the commercial software platform...
April 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28338345/challenges-and-driving-forces-for-business-plans-in-biobanking
#18
Tanja Macheiner, Berthold Huppertz, Michaela Bayer, Karine Sargsyan
BACKGROUND: Due to increased utilization of biospecimens for research and emergence of new technologies, the availability and quality of biospecimens and their collection are coming more and more into focus. However, the long-term economic situation of biobanks is still mostly unclear. Also, the common sustainable utilization of various international biobanks is challenging due to local differences in sample processing, law and ethics. AIM: This article discusses possible strategies to achieve a sustainable utilization of biospecimens as part of the business plan of biobanks...
April 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28338336/the-journey-commemorating-bio-s-15th-anniversary
#19
John G Baust, Sheryl Nale
No abstract text is available yet for this article.
April 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28332850/integrating-molecular-control-to-improve-cryopreservation-outcome
#20
John G Baust, Kristi K Snyder, Robert Van Buskirk, John M Baust
Cryopreservation (CP) is a critical component in enabling on-demand access to biological material (macromolecules, cells, and tissues), yet CP has evolved little over the last several decades. Today's CP processes often yield a suboptimal "product," which has slowed progression in areas such as cell therapy and stem cell research. Recent discoveries focusing on molecular control and buffering of cell stress responses to CP as well as the development of new devices that improve sample freezing and thawing are now providing a path to improve sample quality...
April 2017: Biopreservation and Biobanking
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