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Biopreservation and Biobanking

Peggy Manders, Tessa M A Peters, Ariaan E Siezen, Iris A L M van Rooij, Roger Snijder, Dorine W Swinkels, Gerhard A Zielhuis
INTRODUCTION: Current guidelines for clinical biobanking have a strong focus on obtaining, handling, and storage of biospecimens. However, to allow for research tying biomarker analysis to clinical decision making, there should be more focus on collection of data on donor characteristics. Therefore, our aim was to develop a stepwise procedure to define a framework as a tool to help start the data collection process in clinical biobanking. MATERIALS AND METHODS: The Radboud Biobank (RB) is a central clinical biobanking facility designed in accordance with the standards set by the Parelsnoer Institute, a Dutch national biobank originally initiated with eight different disease cohorts...
February 13, 2018: Biopreservation and Biobanking
Daniel Simeon-Dubach, Zisis Kozlakidis
No abstract text is available yet for this article.
February 2, 2018: Biopreservation and Biobanking
Shannon J McCall, Philip A Branton, Victoria M Blanc, Sarah M Dry, Julie M Gastier-Foster, James H Harrison, Scott D Jewell, Rajesh C Dash, Rebecca C Obeng, Joan Rose, Dawna L Mateski, Albi Liubinskas, James A Robb, Nilsa C Ramirez, Kathi Shea
The College of American Pathologists (CAP) developed the Biorepository Accreditation Program (BAP) in 2012. This program integrates best practices from the International Society for Biological and Environmental Biorepositories, the National Cancer Institute, the Organisation for Economic Cooperation and Development, the Center for Medicare and Medicaid Services, and the CAP Laboratory Accreditation Program. The goal of this elective program is to provide requirements for standardization in biorepository processes that will result in high-quality specimens that can be used to support research, drug discovery, and personalized medicine...
February 2, 2018: Biopreservation and Biobanking
Koh Furuta, Clare M Allocca, Brent Schacter, Marianna J Bledsoe, Nilsa C Ramirez
Recent advances in biotechnology are making it possible to advance science and improve healthcare with increasing speed and precision. Biobanking, as a foundation of the biotechnology infrastructure, is critical to the assurance of quality for many of the key components for these advancing technologies in both the human and nonhuman domains. Biobanking must advance to support the increased complexity and required precision needs of biological resources. Standards development can provide an important link for the research and development community by providing tools to ensure quality, fitness-for-purpose, and reproducibility in biobanking...
February 2, 2018: Biopreservation and Biobanking
Lori D Campbell, Jonas J Astrin, Yvonne DeSouza, Judith Giri, Ashokkumar A Patel, Melissa Rawley-Payne, Amanda Rush, Nicole Sieffert
An increased need for specimens of reliable and consistent quality for research purposes requires the development of standardized policies and practices for the collection, handling, storage, retrieval, and distribution of specimens and specimen-related data. Providers of specimen resources should strive to incorporate new technologies and state-of-the-science approaches and thus ensure the availability of fit-for-purpose research specimens. Strategies to achieve quality outcomes and performance improvements often include adherence to established standards and implementation of best practices...
February 2, 2018: Biopreservation and Biobanking
Fay Betsou, Roberto Bilbao, Jamie Case, Rodrigo Chuaqui, Judith Ann Clements, Yvonne De Souza, Annemieke De Wilde, Jörg Geiger, William Grizzle, Fiorella Guadagni, Elaine Gunter, Stacey Heil, Michael Kiehntopf, Iren Koppandi, Sabine Lehmann, Loes Linsen, Jacqueline Mackenzie-Dodds, Rocio Aguilar Quesada, Riad Tebbakha, Teresa Selander, Katheryn Shea, Mark Sobel, Stella Somiari, Demetri Spyropoulos, Mars Stone, Gunnel Tybring, Klara Valyi-Nagy, Lalita Wadhwa
No abstract text is available yet for this article.
January 29, 2018: Biopreservation and Biobanking
Jesús García-Martínez, Daniel López Lacomba, Angel Castaño Pascual
The conservation of microorganisms is essential for their in-depth study. However, today's most widely used conservation methods, based on the use of distilled water, soil, oils, or silica, do not guarantee the stability of fungal cells, especially dermatophytes. This problem led us to evaluate the conservation capacity of a cryogenic vials system containing glass beads covered in a cryopreservant hypertonic solution as an alternative method of storage of fungal cells at -80°C. Up to 570 strains of fungi belonging to 27 different species, isolated from clinical samples, were inoculated into cryotubes containing 25 glass beads covered in a cryopreserving hypertonic solution...
January 25, 2018: Biopreservation and Biobanking
Sheila O'Donoghue, Lise Matzke, Peter Watson
No abstract text is available yet for this article.
January 24, 2018: Biopreservation and Biobanking
Seyedeh Zeynab Sadr, Roya Fatehi, Saman Maroufizadeh, Christiani Andrade Amorim, Bita Ebrahimi
In vitro culture of ovarian follicles is a new technique in reproductive technology, which helps in understanding the process of folliculogenesis. The in vitro culture of follicles could be carried out using three-dimensional (3D) natural scaffolds that mimic the ovarian tissue stroma. Selection of the right matrix and culture media in these scaffolds could increase the survival and maturation of the follicles. In this work, the applicability of matrigel-alginate (MA) and fibrin-alginate (FA) 3D scaffolds for folliculogenesis was assessed...
January 24, 2018: Biopreservation and Biobanking
Maksim V Puzanov, Liudmila B Vasilyeva, Polina V Popova, Elena N Grineva, Renata I Dmitrieva
It is widely accepted that endothelial dysfunction (ED) is a common feature and a risk factor for cardiovascular diseases and metabolic disorders. Cultures of human umbilical vein endothelial cells (HUVECs) are routinely used in cell-based models to study in vitro molecular and cellular mechanisms of development of different aspects of ED. The methods of the HUVEC extraction and expansion are well developed and standardized. However, when large collections of samples are needed for certain projects, or when samples from a rare population of patients should be collected for future experimental use, HUVEC samples should be transferred to a biobank to be saved in liquid nitrogen for a long period of time until the required collection is completed...
January 24, 2018: Biopreservation and Biobanking
Petr Holub, Florian Kohlmayer, Fabian Prasser, Michaela Th Mayrhofer, Irene Schlünder, Gillian M Martin, Sara Casati, Lefteris Koumakis, Andrea Wutte, Łukasz Kozera, Dominik Strapagiel, Gabriele Anton, Gianluigi Zanetti, Osman Ugur Sezerman, Maimuna Mendy, Dalibor Valík, Marialuisa Lavitrano, Georges Dagher, Kurt Zatloukal, GertJan B van Ommen, Jan-Eric Litton
The known challenge of underutilization of data and biological material from biorepositories as potential resources for medical research has been the focus of discussion for over a decade. Recently developed guidelines for improved data availability and reusability-entitled FAIR Principles (Findability, Accessibility, Interoperability, and Reusability)-are likely to address only parts of the problem. In this article, we argue that biological material and data should be viewed as a unified resource. This approach would facilitate access to complete provenance information, which is a prerequisite for reproducibility and meaningful integration of the data...
January 23, 2018: Biopreservation and Biobanking
Fay Betsou
No abstract text is available yet for this article.
January 23, 2018: Biopreservation and Biobanking
Sepideh Jalilzadeh-Tabrizi, Younes Pilehvar-Soltanahmadi, Effat Alizadeh, Shahriar Alipour, Mehdi Dadashpour, Kazem Nejati-Koshki, Nosratollah Zarghami
BACKGROUND: Emu oil (EO) with anti-inflammatory, antioxidative, and wound healing properties can be blended for preparing bioactive nanofibrous scaffold. Adipose tissue-derived stem cells (ADSCs) are promising candidates for tissue engineering, and preserving their stemness potential is vital for further therapeutic applications. AIM: The aim of this study was to fabricate EO-blended nanofiber and investigate its effect on proliferation, survival, and stemness preservation of ADSCs...
January 22, 2018: Biopreservation and Biobanking
Zisis Kozlakidis
No abstract text is available yet for this article.
January 9, 2018: Biopreservation and Biobanking
Wenlong Carl Chen, Robyn Kerr, Andrew May, Babongile Ndlovu, Anelisa Sobalisa, Sanelisiwe T Duze, Lavania Joseph, Christopher G Mathew, Chantal Babb de Villiers
Long-term storage of whole blood can affect the integrity of DNA if it is not done under optimal conditions. The aim of this study was to determine whether long-term storage (2-19 years) of whole blood samples at -30°C had a negative effect on the quality or quantity of genomic DNA that could be recovered at extraction. Genomic DNA was isolated from 2758 whole blood samples collected in 4 mL EDTA vacutainers from 1997 to 2012. DNA was extracted using the Qiagen® FlexiGene® DNA kit. The average storage duration at -30°C was 12 years...
January 3, 2018: Biopreservation and Biobanking
Collin Dickerson, Yanshen Hsu, Sandra Mendoza, Iman Osman, Jennifer Ogilvie, Kepal Patel, Andre L Moreira
Biospecimen quality can vary depending on many pre- and post-collection variables. In this study, we consider a natural disaster as a post-collection variable that may have compromised the quality of frozen tissue specimens. To investigate this possible link, we compared the quality of nucleic acids, the level of antigenicity, and the preservation of histology from frozen specimens collected before and after the power outage caused by Hurricane Sandy. To analyze nucleic acid quality, we extracted both DNA and RNA and performed capillary electrophoresis to compare the quality and concentrations of the nucleic acids...
January 3, 2018: Biopreservation and Biobanking
Wanyun Zuo, Bingyu Xie, Chenglong Li, Yuhan Yan, Yangyi Zhang, Wei Liu, Jufang Huang, Dan Chen
Endometrial mesenchymal stem cells (enMSCs) are a class of novel adult stem cells with self-renewal capacity, differentiation potential, low immunogenicity, low tumorigenicity, and other biological characteristics. Since the discovery of enMSCs, they have become a hot research topic. In recent years, research on enMSC isolation and application have made great progress. In this review, we focus on the clinical applications of this cell type. The latest research on the applications of enMSCs in the immune, gynecological, cardiovascular, digestive, nervous systems and metabolic diseases, as well as biobanking of enMSCs will be reviewed...
December 21, 2017: Biopreservation and Biobanking
Yanjuan Jia, Hui Xu, Yonghong Li, Chaojun Wei, Rui Guo, Fang Wang, Yu Wu, Jing Liu, Jing Jia, Junwen Yan, Xiaoming Qi, Yuanting Li, Xiaoling Gao
Although the Ficoll-Paque method is classically used to isolate peripheral blood mononuclear cells (PBMCs), modifications in this method are required for a more rapid and economic output for biobanks and clinical laboratories, particularly in developing countries. In this study, we addressed this issue by modifying the Ficoll-Paque method for the isolation of PBMCs or mononuclear cells from the peripheral and the umbilical cord blood of healthy and diseased (infected, anemic, and chronic obstructive pulmonary disease) adult individuals...
December 12, 2017: Biopreservation and Biobanking
Rod Ling, Amanda Rush, Candace Carter, Jane Carpenter, Peter H Watson, Jennifer A Byrne, Andrew Searles
Biobanks face increasing demands for research materials of consistent quality, which can be used in collaborative studies. Several countries and some international agencies have made formal efforts to standardize biobank operations and outputs. These include the establishment of best practice guidelines for collection management, and certification programs. Such guidelines and programs increase biobanks' opportunities for participation in high impact research and funding. However, they also impose economic and time costs, which may burden biobanks...
December 7, 2017: Biopreservation and Biobanking
Alana A Borges, Gabriela P O Lira, Lucas E Nascimento, Luiza B Queiroz Neta, Maria V O Santos, Moacir F Oliveira, Alexandre R Silva, Alexsandra F Pereira
Skin vitrification is a promising and alternative tool for the conservation of biodiversity, especially for wild mammals, such as collared peccaries. Several factors can affect the success of this procedure, such as the cryoprotectant solution used. Therefore, this study was carried out to compare the efficiency of various vitrification solutions for recovery of viable cells after in vitro culture of cryopreserved skin tissues derived from the collared peccary, aiming to study the application in biobanking, where cellular use is not immediately required...
December 7, 2017: Biopreservation and Biobanking
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