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Biopreservation and Biobanking

Helmuth Haslacher, Marlene Gerner, Philipp Hofer, Andreas Jurkowitsch, Johannes Hainfellner, Renate Kain, Oswald F Wagner, Thomas Perkmann
BACKGROUND AND AIM: It is increasingly recognized that biomedical research has serious reproducibility issues, which could be overcome at least in part by standardized processing of biomaterials. Therefore, professional biobanks have emerged, positively influencing sample and data quality. However, quantitative data about a biobank's contribution to published results are still hard to find, although they could serve as valuable benchmark figures for the community. We therefore aimed to report usage data from the MedUni Wien Biobank facility regarding its prospective fluid cohorts...
October 18, 2018: Biopreservation and Biobanking
Lucie Gavin-Plagne, Loris Commin, Pierre Bruyère, Samuel Buff, Thierry Joly
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility...
October 16, 2018: Biopreservation and Biobanking
Andrey Nikolayevich Khudyakov, Larisa Georgievna Kuleshova, Oksana Olegovna Zaitseva, Marta Igorevna Sergushkina, Konstantin Aleksandrovich Vetoshkin, Tatyana Vitalyevna Polezhaeva
The ability of various pectin polysaccharides to modify the morphological structure of ice during the phase transitions from water to ice was studied. Pectins were isolated from Sosnowsky's hogweed Heracleum sosnowskyi Manden (heracleuman-N6HS), tansy Tanacetum vulgare L. (tanacetan-N7TVF), and Rauwolfia serpentina Benth callus (rauwolfian-N8RS). Pectins were isolated by multistep extraction. The effect of pectins was assessed using osmometry, thermographic analysis, and cryomicroscopy. A concentrate of leukocytes was used as the sample for the subsequent freezing step...
October 9, 2018: Biopreservation and Biobanking
Yanhong Liu, Hong Gao, Yue Hu, Jie Ding, Meiling Ge, Qing Ye
AIM: To ensure that sample quality meets the requirements of experimental research, the gynecology and obstetrics biobank of the Nanjing Drum Tower hospital designed different quality control methods for relevant types of samples. A range of quality control procedures has been formulated. METHODS: The sample types were frozen tissue, paraffin-embedded tissue, optimal cutting temperature (OCT)-embedded tissue, plasma, buffy coat, serum, blood clots, and urine. Different categories of samples from a random selection of 1% of cases were analyzed for quality control experiments: (i) frozen tissue, buffy coat, and blood clots: RNA and DNA were extracted and the concentration, purity, and integrity were determined; (ii) paraffin-embedded tissue: morphological observations were made after hematoxylin-eosin staining and immunohistochemical detection of β-actin or CD10; (iii) OCT-embedded tissue: hematoxylin-eosin staining and immunofluorescence detection of β-actin; and (iv) frozen tissue samples derived from different organs of 18 fetal autopsy specimens with different cold ischemia times (CITs), 0-12 hours, 12-18 hours, 18-24 hours, and 24-48 hours, were chosen to study RNA quality...
October 6, 2018: Biopreservation and Biobanking
Hong Gao, Yanhong Liu, Jie Ding, Jun Yang, Biao Zhang, Yue Hu, Meiling Ge, Qing Ye
BACKGROUND: The preservation of placental and fetal tissues will contribute to studying the pathogenesis of high-risk pregnancy diseases. However, few studies have focused on the effects of different preservation methods and cold ischemia time (CIT) on the quality of nucleic acids. An available quality control (QC) strategy will be beneficial to evaluate these effects for high-risk pregnancy biobanks. METHODS: We established an evaluation strategy of nucleic acid QC by analyzing total RNA and genomic DNA (gDNA)...
September 26, 2018: Biopreservation and Biobanking
Guiulfo Mamani-Mango, Milagros Moina Gonzales, Martín Ramos Hidalgo, José Mendoza Mallma, Jaime Ruiz Béjar, Victoria Rivas Palma, Edwin Mellisho Salas
In alpacas, improvement of reproductive efficiency of male camelids is limited by the small testicular size, low spermatozoa production, and low quality of semen. In this study we aim to evaluate the effect of two extenders and two freezing rates on post-thaw quality of sperm recovered from alpaca epididymis with two methods (flushing and mincing), and to evaluate the in vitro fertilization (IVF) capacity of frozen sperm selected with two different selection methods (washing and swim-up). Sperm samples were processed with Tris-egg yolk or Bioxcell® extenders and frozen with slow freezing and fast freezing...
September 26, 2018: Biopreservation and Biobanking
Sergei Amstislavsky, Valentina Mokrousova, Eugeny Brusentsev, Konstantin Okotrub, Pierre Comizzoli
Lipids are among the most abundant and essential cell components. Specifically, cytoplasmic lipid droplets (LDs) play crucial roles in cellular energy homeostasis. The foci of this review are (1) the composition and roles of lipids during oocyte maturation and early embryonic development, (2) possible causes of cryoinjuries in lipid-rich oocytes/embryos, and (3) ways to overcome these detrimental effects. Recent reports show that LDs in oocytes and embryos are not only energy depots but also are active organelles, possessing many other functions...
September 25, 2018: Biopreservation and Biobanking
Wim Ammerlaan, Johanna Trouet, Michael C Sachs, Ping Guan, Latarsha Carithers, Pauline Lambert, Sonia Frasquilho, Laurent Antunes, Olga Kofanova, Daniel Rohrer, Dana R Valley, Alex Blanski, Scott Jewell, Helen Moore, Fay Betsou
Although there are millions of formalin-fixed paraffin-embedded (FFPE) tissue blocks potentially available for scientific research, many are of questionable quality, partly due to unknown fixation conditions. We analyzed FFPE tissue biospecimens as part of the NCI Biospecimen Preanalytical Variables (BPV) program to identify microRNA (miRNA) markers for fixation time. miRNA was extracted from kidney and ovary tumor FFPE blocks (19 patients, cold ischemia ≤2 hours) with 6, 12, 24, and 72 hours fixation times, then analyzed using the WaferGen SmartChip platform (miRNA chip with 1036 miRNA targets)...
September 19, 2018: Biopreservation and Biobanking
Jessica Pereira, Xavier Ferraretto, Catherine Patrat, Anne Meddahi-Pellé
INTRODUCTION: Cryopreservation provides an efficient way to preserve cells for a broad range of medical applications, including cell therapy. In clinical practice, cells are frozen in solutions containing dimethyl sulfoxide (DMSO) cryoprotectant agents (CPAs) to reduce their damage during the cooling process. In the current cell preservation methods, polysaccharides such as dextran, a nonpenetrating CPA, are used. However, the cell viability decreases when the solution concentration in polysaccharides increases...
September 5, 2018: Biopreservation and Biobanking
Pariya Saberi, Mohsen Forouzanfar, Mohammad Hossein Nasr-Esfahani
Embryos can be produced in nonheat stress conditions and transported by air to designated destinations using hypothermic and chilled storage procedures. Therefore, improvement of efficiency of these processes may have an important impact on the dairy industry. The aim of this study was to evaluate the viability of embryos treated with ROCK inhibitor (Y-27632) during 4 days of hypothermic storage of goat blastocysts. In this study, vitrification was used as a standard model of cryopreservation. Treatment with ROCK inhibitor (Y-27632+ ) significantly improved the re-expansion rate of blastocyst postwarming compared with treatment with Y-26732- , but it was lower than the re-expansion rate in vitrified/warmed blastocysts...
August 22, 2018: Biopreservation and Biobanking
Michaela Th Mayrhofer, Irene Schlünder
With the ethical, legal, and societal issues (ELSI) Knowledge Base, we introduce a key element of the Biobanking and Biomolecular Resources Research Infrastructure-European Research Infrastructure Consortium (BBMRI-ERIC) Common Service ELSI, which provides ethical, legal, and societal support for researchers and biobankers involved in transnational research. In contrast to the customized support provided by the ELSI Helpdesk, the ELSI Knowledge Base will be available to the user on a self-serve basis. The information that is made available through a knowledge base comes from multiple sources, usually from several expert contributors who are well versed in the subject matter...
August 13, 2018: Biopreservation and Biobanking
José Carlos Ferreira-Silva, Sarah Romini Lima Basto, Marcelo Tigre Moura, Jorge Motta Rocha, Leopoldo Mayer Freitas Neto, José Pompeu Santos Filho, Manoel Lopes Silva Filho, Marcos Antonio Lemos Oliveira
The work described here aimed to verify the efficiency of different extenders for cryopreservation of equine semen using sperm motility and acrosin activity as spermatic parameters. The semen was fractioned into two equal parts and resuspended in an 11% lactose solution in a 1:1 proportion, where it remained for 20 minutes at room temperature. The semen was centrifuged at 600 g for 10 minutes, and after the second centrifugation, each pellet received the freezing extender (Merck or Zorlesco) and was loaded into 4 mL straws...
July 30, 2018: Biopreservation and Biobanking
Katherine O'Donnell, Suzanne Sharrock
Originating in Europe in the 16th century, botanic gardens are found in nearly every country in the world. Botanic gardens have had various roles during this time, including as physic gardens, as adaptation centers for commercial crops, as pleasure gardens, and more recently as conservation institutions. The role of botanic gardens in the conservation of Crop Wild Relatives is becoming increasingly important. At least 6000 taxa related to 68 crop genera are maintained by the world's botanic gardens with several gardens having specialist collections...
October 2018: Biopreservation and Biobanking
Andreas W Ebert, Logotonu M Waqainabete
This review article gives an account of the origin, domestication, and dispersal of taro, a staple food crop in many countries in the humid tropics and subtropics. Genetic diversity studies indicated that distinct gene pools exist in all the regions where taro may be naturally distributed-the Indian subcontinent, China, Southeast Asia, and in Oceania. The Asian gene pool presented the highest genetic diversity. Diploid taro is prevalent in the Pacific Islands, while both diploids and triploids are found in mainland Asia...
October 2018: Biopreservation and Biobanking
Elena Popova
No abstract text is available yet for this article.
October 2018: Biopreservation and Biobanking
Alfredo Augusto Cunha Alves, Vânia Cristina Rennó Azevedo
Brazil is one of the most biodiverse countries on Earth, holding ∼10% of the world's vascular plant species. Despite that, Brazilian agriculture is highly dependent on genetic resources originating from other countries. Embrapa (Brazilian Agricultural Research Corporation) is the governmental institution that, since 1973, has been responsible for the introduction and conservation of genetic resources in Brazil. In this article, we report on the experiences that Embrapa has faced over the past 45 years to build and improve a national network for the preservation of plant genetic resources under the coordination of Embrapa Genetic Resources & Biotechnology (CENARGEN), one of the 42 Embrapa decentralized units...
October 2018: Biopreservation and Biobanking
Noelle L Anglin, Ahmed Amri, Zakaria Kehel, Dave Ellis
Genebanks are responsible for collecting, maintaining, characterizing, documenting, and distributing plant genetic resources for research, education, and breeding purposes. The rationale for requests of plant materials varies highly from areas of anthropology, social science, small-holder farmers, the commercial sector, rehabilitation of degraded systems, all the way to crop improvement and basic research. Matching "the right" accessions to a particular request is not always a straightforward process especially when genetic resource collections are large and the user does not already know which accession or even which species they want to study...
October 2018: Biopreservation and Biobanking
Michael Halewood, Isabel Lopez Noriega, Dave Ellis, Carolina Roa, Mathieu Rouard, Ruaraidh Sackville Hamilton
This article describes how CGIAR centers and partners are using genomic sequence information to promote the conservation and sustainable use of crop genetic diversity, and to generate and share benefits derived from those uses. The article highlights combined institutional, and benefit-sharing-related challenges that need to be addressed to support expanded use of digital sequence information in agricultural research and development.
October 2018: Biopreservation and Biobanking
Katherine J Whitehouse, Olorunnisola F Owoborode, Olufemi O Adebayo, Olaniyi A Oyatomi, Amudalat B Olaniyan, Michael T Abberton, Fiona R Hay
Maximizing seed longevity is important for genebanks to efficiently manage their accessions, reducing the frequency of costly regeneration cycles and the loss of genetic integrity. Research on rice seeds has shown that subsequent longevity in air-dry storage can be improved by drying seeds, which are metabolically active at harvest (moisture contents above a critical value close to 16.5%), for an initial period at a higher temperature (40°C-60°C) than that currently recommended by the current genebank standards (5°C-20°C)...
October 2018: Biopreservation and Biobanking
Åsmund Asdal, Luigi Guarino
No abstract text is available yet for this article.
October 2018: Biopreservation and Biobanking
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