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International Journal of Oral Science

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https://www.readbyqxmd.com/read/27910940/supportive-angiogenic-and-osteogenic-differentiation-of-mesenchymal-stromal-cells-and-endothelial-cells-in-monolayer-and-co-cultures
#1
Florian Böhrnsen, Henning Schliephake
Sites of implantation with compromised biology may be unable to achieve the required level of angiogenic and osteogenic regeneration. The specific function and contribution of different cell types to the formation of prevascularized, osteogenic networks in co-culture remains unclear. To determine how bone marrow-derived mesenchymal stromal cells (BMSCs) and endothelial cells (ECs) contribute to cellular proangiogenic differentiation, we analysed the differentiation of BMSCs and ECs in standardized monolayer, Transwell and co-cultures...
December 2, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27857074/second-premolar-agenesis-is-associated-with-mandibular-form-a-geometric-morphometric-analysis-of-mandibular-cross-sections
#2
Michael H Bertl, Kristina Bertl, Manuel Wagner, André Gahleitner, Andreas Stavropoulos, Christian Ulm, Philipp Mitteroecker
The aim of this study was to compare mandibular form (i.e., size and shape) between patients with agenesis of the lower second premolar (P2) and a control group with no agenesis. Three hypotheses were tested: (H1) agenesis causes a change in mandibular morphology because of inadequate alveolar ridge development in the area of the missing tooth (mandibular plasticity); (H2) agenesis is caused by spatial limitations within the mandible (dental plasticity); and (H3) common genetic/epigenetic factors cause agenesis and affect mandibular form (pleiotropy)...
November 18, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27811847/novel-rechargeable-calcium-phosphate-nanoparticle-containing-orthodontic-cement
#3
Xian-Ju Xie, Dan Xing, Lin Wang, Han Zhou, Michael D Weir, Yu-Xing Bai, Hockin Hk Xu
White spot lesions (WSLs), due to enamel demineralization, occur frequently in orthodontic treatment. We recently developed a novel rechargeable dental composite containing nanoparticles of amorphous calcium phosphate (NACP) with long-term calcium (Ca) and phosphate (P) ion release and caries-inhibiting capability. The objectives of this study were to develop the first NACP-rechargeable orthodontic cement and investigate the effects of recharge duration and frequency on the efficacy of ion re-release. The rechargeable cement consisted of pyromellitic glycerol dimethacrylate (PMGDM) and ethoxylated bisphenol A dimethacrylate (EBPADMA)...
November 4, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27811846/primer-containing-dimethylaminododecyl-methacrylate-kills-bacteria-impregnated-in-human-dentin-blocks
#4
Chen Chen, Lei Cheng, Michael D Weir, Nancy J Lin, Sheng Lin-Gibson, Xue-Dong Zhou, Hockin Hk Xu
Antibacterial dimethylaminododecyl methacrylate (DMADDM) was recently synthesized. The objectives of this study were to: (1) investigate antibacterial activity of DMADDM-containing primer on Streptococcus mutans impregnated into dentin blocks for the first time, and (2) compare the antibacterial efficacy of DMADDM with a previous quaternary ammonium dimethacrylate (QADM). Scotchbond Multi-Purpose (SBMP) bonding agent was used. DMADDM and QADM were mixed into SBMP primer. Six primers were tested: SBMP control primer P, P+2...
November 4, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27811845/dental-pulp-stem-cells-express-tendon-markers-under-mechanical-loading-and-are-a-potential-cell-source-for-tissue-engineering-of-tendon-like-tissue
#5
Yu-Ying Chen, Sheng-Teng He, Fu-Hua Yan, Peng-Fei Zhou, Kai Luo, Yan-Ding Zhang, Yin Xiao, Min-Kui Lin
Postnatal mesenchymal stem cells have the capacity to differentiate into multiple cell lineages. This study explored the possibility of dental pulp stem cells (DPSCs) for potential application in tendon tissue engineering. The expression of tendon-related markers such as scleraxis, tenascin-C, tenomodulin, eye absent homologue 2, collagens I and VI was detected in dental pulp tissue. Interestingly, under mechanical stimulation, these tendon-related markers were significantly enhanced when DPSCs were seeded in aligned polyglycolic acid (PGA) fibre scaffolds...
November 4, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27767053/globoside-accelerates-the-differentiation-of-dental-epithelial-cells-into-ameloblasts
#6
Takashi Nakamura, Yuta Chiba, Masahiro Naruse, Kan Saito, Hidemitsu Harada, Satoshi Fukumoto
Tooth crown morphogenesis is tightly regulated by the proliferation and differentiation of dental epithelial cells. Globoside (Gb4), a globo-series glycosphingolipid, is highly expressed during embryogenesis as well as organogenesis, including tooth development. We previously reported that Gb4 is dominantly expressed in the neutral lipid fraction of dental epithelial cells. However, because its functional role in tooth development remains unknown, we investigated the involvement of Gb4 in dental epithelial cell differentiation...
October 21, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27740612/alanine-racemase-is-essential-for-the-growth-and-interspecies-competitiveness-of-streptococcus-mutans
#7
Yuan Wei, Wei Qiu, Xue-Dong Zhou, Xin Zheng, Ke-Ke Zhang, Shi-Da Wang, Yu-Qing Li, Lei Cheng, Ji-Yao Li, Xin Xu, Ming-Yun Li
D-alanine (D-Ala) is an essential amino acid that has a key role in bacterial cell wall synthesis. Alanine racemase (Alr) is a unique enzyme that interconverts L-alanine and D-alanine in most bacteria, making this enzyme a potential target for antimicrobial drug development. Streptococcus mutans is a major causative factor of dental caries. The factors involved in the survival, virulence and interspecies interactions of S. mutans could be exploited as potential targets for caries control. The current study aimed to investigate the physiological role of Alr in S...
October 14, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27660048/colour-stabilities-of-three-types-of-orthodontic-clear-aligners-exposed-to-staining-agents
#8
Chen-Lu Liu, Wen-Tian Sun, Wen Liao, Wen-Xin Lu, Qi-Wen Li, Yunho Jeong, Jun Liu, Zhi-He Zhao
The aim of this study was to evaluate and compare the colour stabilities of three types of orthodontic clear aligners exposed to staining agents in vitro. Sixty clear orthodontic aligners produced by three manufacturers (Invisalign, Angelalign, and Smartee) were immersed in three staining solutions (coffee, black tea, and red wine) and one control solution (distilled water). After 12-h and 7-day immersions, the aligners were washed in an ultrasonic cleaner and measured with a colourimeter. The colour changes (ΔE*) were calculated on the basis of the Commission Internationale de I'Eclairage L*a*b* colour system (CIE L*a*b*), and the results were then converted into National Bureau of Standards (NBS) units...
September 23, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27680288/cetylpyridinium-chloride-mouth-rinses-alleviate-experimental-gingivitis-by-inhibiting-dental-plaque-maturation
#9
Fei Teng, Tao He, Shi Huang, Cun-Pei Bo, Zhen Li, Jin-Lan Chang, Ji-Quan Liu, Duane Charbonneau, Jian Xu, Rui Li, Jun-Qi Ling
Oral rinses containing chemotherapeutic agents, such as cetylpyridinium chloride (CPC), can alleviate plaque-induced gingival infections, but how oral microbiota respond to these treatments in human population remains poorly understood. Via a double-blinded, randomised controlled trial of 91 subjects, the impact of CPC-containing oral rinses on supragingival plaque was investigated in experimental gingivitis, where the subjects, after a 21-day period of dental prophylaxis to achieve healthy gingivae, received either CPC rinses or water for 21 days...
September 29, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27585820/saliva-in-the-diagnosis-of-diseases
#10
REVIEW
Chen-Zi Zhang, Xing-Qun Cheng, Ji-Yao Li, Ping Zhang, Ping Yi, Xin Xu, Xue-Dong Zhou
Saliva is secreted from the salivary glands and has multiple functions, including mouth cleaning and protection, antibacterial effects and digestion. With the rapid advancement in salivaomics, saliva is well recognized as a pool of biological markers. Saliva, as a non-invasive and safe source, could be a substitute for blood in the diagnosis and prognosis of diseases. This review summarizes the latest advancements in saliva-related studies and addresses the potential value of saliva in the early diagnosis of oral diseases, such as dental caries and periodontal disease, as well as cancer, diabetes and other systemic disorders...
September 29, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27515277/oral-polymorphonuclear-neutrophil-characteristics-in-relation-to-oral-health-a-cross-sectional-observational-clinical-study
#11
Patrick Rijkschroeff, Ineke D C Jansen, Fridus A van der Weijden, Bart J F Keijser, Bruno G Loos, Elena A Nicu
Polymorphonuclear neutrophils (PMNs) have a major role in the innate immune system. However, little is known about PMN contribution in relation to oral health. The objective of this study was to investigate the numbers and functional characteristics of oral PMNs (oPMNs) compared with circulatory PMNs (cPMNs). Oral rinse and venous blood samples were obtained from 268 systemically and orally healthy volunteers in a cross-sectional observational study. PMN counts, cell cycle analysis and cellular activation state were investigated...
September 29, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27445089/effect-of-liner-and-porcelain-application-on-zirconia-surface-structure-and-composition
#12
Tariq F Alghazzawi, Gregg M Janowski
The purpose of this study was to determine if there is an effect of liner and porcelain application (layering and pressing techniques) on the surface of yttria-stabilized tetragonal zirconia polycrystals (Y-TZP), which were exposed to permutations of liner, layered porcelain, and pressed porcelain. Scanning electron microscope (SEM)/energy dispersive spectroscope (EDS) was used to identify changes in composition and microstructure after removing liner and porcelain with hydrofluoric acid. Simulated aging was also conducted to determine the effect of liner and porcelain on low-temperature degradation...
September 29, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27388806/corrosion-behaviours-of-the-dental-magnetic-keeper-complexes-made-by-different-alloys-and-methods
#13
Min-Ke Wu, Ning Song, Fei Liu, Liang Kou, Xiao-Wen Lu, Min Wang, Hang Wang, Jie-Fei Shen
The keeper and cast dowel-coping, as a primary component for a magnetic attachment, is easily subjected to corrosion in a wet environment, such as the oral cavity, which contains electrolyte-rich saliva, complex microflora and chewing behaviour and so on. The objective of this in vitro study was to examine the corrosion resistance of a dowel and coping-keeper complex fabricated by finish keeper and three alloys (cobalt-chromium, CoCr; silver-palladium-gold, PdAu; gold-platinum, AuPt) using a laser-welding process and a casting technique...
September 29, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27341388/engineered-three-dimensional-rabbit-oral-epithelial-mesenchymal-muscular-hybrid-sheets
#14
Shigeki Yamane, Kazunari Higa, Takashi Umezawa, Masamitsu Serikawa, Jun Shimazaki, Shinichi Abe
Regenerative muscles are required for swallowing and mastication, and are important for functional recovery from diseases involving oral muscular defects. Therefore, we generated three-layer hybrid sheets, similar to oral mucosal structures containing submucosal muscles, using rabbit oral mucosa epithelial, mesenchymal, and myoblastic progenitor cells, and examined the structural proteins. Each cell type was obtained from rabbit oral mucosa using enzymatic digestion. Isolated mesenchymal and myoblastic cells were multi-differentiated into osteoblasts, adipocytes, and chondrocytes or myotubes...
September 29, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27281037/one-year-water-ageing-of-calcium-phosphate-composite-containing-nano-silver-and-quaternary-ammonium-to-inhibit-biofilms
#15
Lei Cheng, Ke Zhang, Chen-Chen Zhou, Michael D Weir, Xue-Dong Zhou, Hockin H K Xu
Dental composites are commonly used restorative materials; however, secondary caries due to biofilm acids remains a major problem. The objectives of this study were (1) to develop a composite containing quaternary ammonium dimethacrylate (QADM), nanoparticles of silver (NAg), and nanoparticles of amorphous calcium phosphate (NACP), and (2) to conduct the first investigation of the mechanical properties, biofilm response and acid production vs water-ageing time from 1 day to 12 months. A 4 × 5 design was utilized, with four composites (NACP-QADM composite, NACP-NAg composite, NACP-QADM-NAg composite, and a commercial control composite), and five water-ageing time periods (1 day, and 3, 6, 9, and 12 months)...
September 29, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27341389/current-advances-in-orthodontic-pain
#16
REVIEW
Hu Long, Yan Wang, Fan Jian, Li-Na Liao, Xin Yang, Wen-Li Lai
Orthodontic pain is an inflammatory pain that is initiated by orthodontic force-induced vascular occlusion followed by a cascade of inflammatory responses, including vascular changes, the recruitment of inflammatory and immune cells, and the release of neurogenic and pro-inflammatory mediators. Ultimately, endogenous analgesic mechanisms check the inflammatory response and the sensation of pain subsides. The orthodontic pain signal, once received by periodontal sensory endings, reaches the sensory cortex for pain perception through three-order neurons: the trigeminal neuron at the trigeminal ganglia, the trigeminal nucleus caudalis at the medulla oblongata and the ventroposterior nucleus at the thalamus...
June 30, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27025259/new-strategies-against-drug-resistance-to-herpes-simplex-virus
#17
REVIEW
Yu-Chen Jiang, Hui Feng, Yu-Chun Lin, Xiu-Rong Guo
Herpes simplex virus (HSV), a member of the Herpesviridae family, is a significant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections. Acyclovir (ACV) and related nucleoside analogues can successfully treat HSV infections, but the emergence of drug resistance to ACV has created a barrier for the treatment of HSV infections, especially in immunocompromised patients. There is an urgent need to explore new and effective tactics to circumvent drug resistance to HSV. This review summarises the current strategies in the development of new targets (the DNA helicase/primase (H/P) complex), new types of molecules (nature products) and new antiviral mechanisms (lethal mutagenesis of Janus-type nucleosides) to fight the drug resistance of HSV...
March 30, 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27025267/short-term-effects-of-repetitive-transcranial-magnetic-stimulation-on-sleep-bruxism-a-pilot-study
#18
Wei-Na Zhou, Hai-Yang Fu, Yi-Fei Du, Jian-Hua Sun, Jing-Lu Zhang, Chen Wang, Peter Svensson, Ke-Lun Wang
The purpose of this study was to investigate the effects of repetitive transcranial magnetic stimulation (rTMS) on patients with sleep bruxism (SB). Twelve patients with SB were included in an open, single-intervention pilot study. rTMS at 1 Hz and an intensity of 80% of the active motor threshold was applied to the 'hot spot' of the masseter muscle representation at the primary motor cortex bilaterally for 20 min per side each day for 5 consecutive days. The jaw-closing muscle electromyographic (EMG) activity during sleep was recorded with a portable EMG recorder at baseline, during rTMS treatment and at follow-up for 5 days...
March 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27025266/a-simple-sensitive-and-non-destructive-technique-for-characterizing-bovine-dental-enamel-erosion-attenuated-total-reflection-fourier-transform-infrared-spectroscopy
#19
In-Hye Kim, Jun Sik Son, Bong Ki Min, Young Kyoung Kim, Kyo-Han Kim, Tae-Yub Kwon
Although many techniques are available to assess enamel erosion in vitro, a simple, non-destructive method with sufficient sensitivity for quantifying dental erosion is required. This study characterized the bovine dental enamel erosion induced by various acidic beverages in vitro using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Deionized water (control) and 10 acidic beverages were selected to study erosion, and the pH and neutralizable acidity were measured. Bovine anterior teeth (110) were polished with up to 1 200-grit silicon carbide paper to produce flat enamel surfaces, which were then immersed in 20 mL of the beverages for 30 min at 37 °C...
March 2016: International Journal of Oral Science
https://www.readbyqxmd.com/read/27025265/effects-of-quaternary-ammonium-chain-length-on-the-antibacterial-and-remineralizing-effects-of-a-calcium-phosphate-nanocomposite
#20
Ke Zhang, Lei Cheng, Michael D Weir, Yu-Xing Bai, Hockin H K Xu
Composites containing nanoparticles of amorphous calcium phosphate (NACP) remineralize tooth lesions and inhibit caries. A recent study synthesized quaternary ammonium methacrylates (QAMs) with chain lengths (CLs) of 3-18 and determined their effects on a bonding agent. This study aimed to incorporate these QAMs into NACP nanocomposites for the first time to simultaneously endow the material with antibacterial and remineralizing capabilities and to investigate the effects of the CL on the mechanical and biofilm properties...
March 2016: International Journal of Oral Science
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